182,479 results match your criteria INSIGHT Newspaper [Journal]


Expectations and experiences of nursing students in supporting new mothers to breastfeed: A descriptive qualitative study.

J Clin Nurs 2019 Feb 20. Epub 2019 Feb 20.

Western Sydney University, School of Nursing and Midwifery, Locked Bag 1797, Penrith, NSW, 2751.

Background: Improving breastfeeding support to mothers has been the focus of several national and international health organizations. There is evidence that theoretical and clinical education improves breastfeeding knowledge and attitudes among nursing and other health professional students prior to clinical placement, to support women who are breastfeeding.

Aims And Objectives: To explore the expectations and experiences of Taiwanese nursing students in supporting breastfeeding on clinical placement. Read More

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http://dx.doi.org/10.1111/jocn.14836DOI Listing
February 2019

Moderate fire severity is best for the diversity of most of the pollinator guilds in Mediterranean pine forests.

Ecology 2019 Feb 20:e02615. Epub 2019 Feb 20.

Laboratory of Biogeography & Ecology, Department of Geography, University of the Aegean, Mytilene, 81100, Greece.

Fire, a frequent disturbance in the Mediterranean, affects pollinator communities. We explored the response of major pollinator guilds to fire severity, across a fire-severity gradient at different spatial scales. We show that the abundance of all pollinator groups responded to fire severity, and that bees and beetles showed in addition a significant species-diversity response. Read More

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http://dx.doi.org/10.1002/ecy.2615DOI Listing
February 2019

DNA Methylation Regulates Corneal Epithelial Wound Healing by Targeting miR-200a and CDKN2B.

Invest Ophthalmol Vis Sci 2019 Feb;60(2):650-660

School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China.

Purpose: DNA methylation is a key epigenetic modification involved in various biological processes and diseases. Corneal epithelial wound healing (CEWH) is essential for restoring corneal integrity and transparency after injury. However, the role of DNA methylation in CEWH remains elusive. Read More

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http://dx.doi.org/10.1167/iovs.18-25443DOI Listing
February 2019

Visual cues that predict intuitive risk perception in the case of HIV.

PLoS One 2019 20;14(2):e0211770. Epub 2019 Feb 20.

Department of Psychology, University of Konstanz, Konstanz, Baden-Württemberg, Germany.

Field studies indicate that people may form impressions about potential partners' HIV risk, yet lack insight into what underlies such intuitions. The present study examined which cues may give rise to the perception of riskiness. Towards this end, portrait pictures of persons that are representative of the kinds of images found on social media were evaluated by independent raters on two sets of data: First, sixty visible cues deemed relevant to person perception, and second, perceived HIV risk and trustworthiness, health, and attractiveness. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0211770PLOS
February 2019

Insight Regarding the Presence of Antagonism-Related Traits and Their Subsequent Impairment in Relation to Psychopathy, Narcissism, and Machiavellianism.

J Pers Disord 2019 Feb 20:1-16. Epub 2019 Feb 20.

University of Georgia, Athens, Georgia.

Clinical theory is skeptical of individuals' ability to recognize the presence, severity, and impact of clinical symptoms and pathological traits (Oltmanns & Powers, 2012); however, empirical work has found moderate self-other convergence for reports of pathological traits and for Antagonism-related personality disorder (PD) constructs (i.e., psychopathy, narcissism, and Machiavellianism), which are characterized by low insight. Read More

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http://dx.doi.org/10.1521/pedi_2019_33_422DOI Listing
February 2019

Ergonomic Benefits of an Angle Grinder With Rotatable Main Handle in a Cutting Task.

Hum Factors 2019 Feb 20:18720819827184. Epub 2019 Feb 20.

University of Tartu, Estonia.

Objective:: The aim of this study was to evaluate the ergonomic benefits of an angle grinder with a rotatable main handle in a cutting task.

Background:: Angle grinder manufacturers rarely address ergonomic features in their advertisements, and if they do, the benefits are expressed in a qualitative manner. Meanwhile, quantitative information about the effects of the device on the worker is required to make informed decisions during tool selection and cumulative trauma prevention. Read More

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http://dx.doi.org/10.1177/0018720819827184DOI Listing
February 2019

Parallel distributed networks resolved at high resolution reveal close juxtaposition of distinct regions.

J Neurophysiol 2019 Feb 20. Epub 2019 Feb 20.

Harvard.

Examination of large-scale distributed networks within the individual reveals details of cortical network organization that are absent in group-averaged studies. One recent discovery is that a distributed transmodal network, often referred to as the 'default network', is comprised of two closely interdigitated networks, only one of which is coupled to posterior parahippocampal cortex. Not all studies of individuals have identified the same networks and questions remain about the degree to which the two networks are separate, particularly within regions hypothesized to be interconnected hubs. Read More

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http://dx.doi.org/10.1152/jn.00808.2018DOI Listing
February 2019

Efficacy and Posttreatment Effects of Therapist-Delivered Cognitive Behavioral Therapy vs Supportive Psychotherapy for Adults With Body Dysmorphic Disorder: A Randomized Clinical Trial.

JAMA Psychiatry 2019 Feb 20. Epub 2019 Feb 20.

Department of Psychiatry, Harvard Medical School, Boston, Massachusetts.

Importance: Cognitive behavioral therapy (CBT), the best-studied treatment for body dysmorphic disorder (BDD), has to date not been compared with therapist-delivered supportive psychotherapy, the most commonly received psychosocial treatment for BDD.

Objective: To determine whether CBT for BDD (CBT-BDD) is superior to supportive psychotherapy in reducing BDD symptom severity and associated BDD-related insight, depressive symptoms, functional impairment, and quality of life, and whether these effects are durable.

Design, Setting, And Participants: This randomized clinical trial conducted at Massachusetts General Hospital and Rhode Island Hospital recruited adults with BDD between October 24, 2011, and July 7, 2016. Read More

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http://dx.doi.org/10.1001/jamapsychiatry.2018.4156DOI Listing
February 2019

A Model of Uric Acid Transport in the Rat Proximal Tubule.

Am J Physiol Renal Physiol 2019 Feb 20. Epub 2019 Feb 20.

Dpt of Pharmacology and Toxicology, University of Lausanne, Switzerland.

The objective of this study was to investigate theoretically the mechanisms underlying uric acid transport in the proximal tubule (PT) of rat kidneys, and their modulation by factors including Na, parathyroid hormone (PTH), Angiotensin II, and SGLT2 inhibitors. To that end, we incorporated the transport of uric acid and its conjugate anion urate in our mathematical model of water and solute transport in the rat PT. The model accounts for parallel urate reabsorption and secretion pathways on apical and basolateral membranes, and their coupling to lactate and alpha-ketoglutarate transport. Read More

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http://dx.doi.org/10.1152/ajprenal.00603.2018DOI Listing
February 2019

Adduct Fluorescence as a Tool to Decipher Sequence Impact on Frameshift Mutations Mediated by a C-Linked C8-Biphenyl-Guanine Lesion.

Chem Res Toxicol 2019 Feb 20. Epub 2019 Feb 20.

Aromatic chemicals can undergo metabolic activation to afford electrophilic species that react at the C8-site of 2'-deoxyguanosine (dG) to generate bulky C8-dG adducts as a basis of initiating carcinogenesis. These DNA lesions have served as models to understand the mechanism of frameshift mutagenesis, especially within CG-dinucleotide repeat sequences, such as NarI (5'-GGCXCC-3', where X = C8-dG adduct). However, there is still limited capacity to predict the likelihood of mutation arising within particular contexts and hence chemistry-based strategies are needed for probing relationships between nucleic acid sequence and structure with replication errors. Read More

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http://dx.doi.org/10.1021/acs.chemrestox.9b00016DOI Listing
February 2019

Thermodynamic signatures of substrate binding for three Thermobifida fusca cellulases with different modes of action.

Biochemistry 2019 Feb 20. Epub 2019 Feb 20.

The enzymatic degradation of recalcitrant polysaccharides is accomplished by synergistic enzyme cocktails of glycoside hydrolases (GHs) and accessory enzymes. Many GHs are processive meaning that they remain attached to the substrate in between subsequent hydrolytic reactions. Cellulases are GHs that catalyze the hydrolysis of cellulose (β-1,4-linked glucose (Glc)). Read More

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http://dx.doi.org/10.1021/acs.biochem.9b00014DOI Listing
February 2019

Potential Diagnostic Imaging of Alzheimer's Disease with Copper-64 Complexes That Bind to Amyloid-β Plaques.

Inorg Chem 2019 Feb 20. Epub 2019 Feb 20.

Centre for Drug Candidate Optimisation, Monash Institute of Pharmaceutical Sciences , Monash University , Parkville , Victoria , Australia , 3052.

Amyloid-β plaques, consisting of aggregated amyloid-β peptides, are one of the pathological hallmarks of Alzheimer's disease. Copper complexes formed using positron-emitting copper radionuclides that cross the blood-brain barrier and bind to specific molecular targets offer the possibility of noninvasive diagnostic imaging using positron emission tomography. New thiosemicarbazone-pyridylhydrazone based ligands that incorporate pyridyl-benzofuran functional groups designed to bind amyloid-β plaques have been synthesized. Read More

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http://dx.doi.org/10.1021/acs.inorgchem.8b03466DOI Listing
February 2019

Regulation of the cell cycle in early mammalian embryos and its clinical implications.

Int J Dev Biol 2019 Feb 6. Epub 2019 Feb 6.

Central European Institute of Technology, Department of Genetics and Reproduction, Veterinary Research Institute, Brno, Czech Republic.

Early embryonic development is characterized by a plethora of very complex and simultaneously operating processes, which are constantly changing cellular morphology and behaviour. After fertilization, blastomeres of the newly created embryo undergo global epigenetic changes and simultaneously initiate transcription from the zygotic genome and differentiation forming separate cell lineages. Some of these mechanisms were extensively studied during the last several decades and valuable insight was gained into how these processes are regulated at the molecular level. Read More

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http://dx.doi.org/10.1387/ijdb.180400maDOI Listing
February 2019

Investigating coordination flexibility of glycerophosphodiesterase (GpdQ) through interactions with mono-, di-, and triphosphoester (NPP, BNPP, GPE, and paraoxon) substrates.

Phys Chem Chem Phys 2019 Feb 20. Epub 2019 Feb 20.

Department of Chemistry, University of Miami, Coral Gables, FL 33146, USA.

In this study, interactions of the catalytically active binuclear form of glycerophosphodiesterase (GpdQ) with four chemically diverse substrates, i.e. NPP (a phosphomonoester), BNPP and GPE (both phosphodiesters), and paraoxon (a phosphotriester) have been investigated using all-atom molecular dynamics (MD) simulations. Read More

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http://dx.doi.org/10.1039/c8cp07031hDOI Listing
February 2019

Mindfulness training for addictions: has neuroscience revealed a brain hack by which awareness subverts the addictive process?

Authors:
Judson Brewer

Curr Opin Psychol 2019 Jan 28;28:198-203. Epub 2019 Jan 28.

Mindfulness Center, Brown University School of Public Health and Warren Alpern School of Medicine, 121 S Main St, Providence, RI 02903, United States. Electronic address:

Addiction is an age-old problem with desire pitted against self-control and will-power. In modern day substances (including food) and experiences (e.g. Read More

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http://dx.doi.org/10.1016/j.copsyc.2019.01.014DOI Listing
January 2019

Molecular constituents and localization of the ionotropic GABA receptor complex in vivo.

Authors:
Susumu Tomita

Curr Opin Neurobiol 2019 Feb 18;57:81-86. Epub 2019 Feb 18.

Department of Cellular and Molecular Physiology, Department of Neuroscience, Yale University School of Medicine, New Haven, CT 06510, United States. Electronic address:

The ionotropic GABA receptor (GABAR) mediates fast inhibition in the brain. The GABAR pore-forming (α, β, and non-α/β) subunits were isolated approximately 30 years ago and have since been the focus of extensive studies. As a result, many properties of GABARs, including subunit assembly and channel and pharmacological properties, have been discovered. Read More

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http://dx.doi.org/10.1016/j.conb.2019.01.017DOI Listing
February 2019

Citrus alkaline extracts prevent fibroblast senescence to ameliorate pulmonary fibrosis via activation of COX-2.

Biomed Pharmacother 2019 Feb 19;112:108669. Epub 2019 Feb 19.

Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China; Department of Respiratory Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing, 210029, China. Electronic address:

Idiopathic pulmonary fibrosis (IPF) is a progressive and ultimately fatal lung disease with a poor prognosis and limited treatment options. The incidence of IPF increases with age, and the mechanisms related to aging such as cellular senescence have been strongly implicated in disease pathology. Therefore, a better understanding of fibroblasts senescence might provide a new therapeutic strategy to prevent and treat pulmonary fibrosis. Read More

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http://dx.doi.org/10.1016/j.biopha.2019.108669DOI Listing
February 2019

Mechanistic insight into diabetic wounds: Pathogenesis, molecular targets and treatment strategies to pace wound healing.

Biomed Pharmacother 2019 Feb 19;112:108615. Epub 2019 Feb 19.

University Institute of Pharmacy, Pt. Ravishankar Shukla University, 492010, Raipur, C.G., India. Electronic address:

Wound management in diabetic patient is of an extreme clinical and social concern. The delayed and impaired healing makes it more critical for research focus. The research on impaired healing process is proceeding hastily evident by new therapeutic approaches other than conventional such as single growth factor, dual growth factor, skin substitutes, cytokine stimulators, cytokine inhibitors, matrix metalloproteinase inhibitors, gene and stem cell therapy, extracellular matrix and angiogenesis stimulators. Read More

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http://dx.doi.org/10.1016/j.biopha.2019.108615DOI Listing
February 2019

Nursing students' commitment and the mediating effect of stress.

Nurse Educ Today 2019 Feb 13;76:172-177. Epub 2019 Feb 13.

UC Business School, University of Canterbury, Private Bag 4800, Christchurch 8140, New Zealand. Electronic address:

Objectives: Knowing there is a shortage of qualified nurses throughout the healthcare industry, it is imperative that program administrators understand the factors that affect nursing students as they select and commit to their academic major. They should consider key influences on potential students as new recruiting and retention tactics are developed and employed. Thus, we develop the current study to understand factors that shape students' commitment to nursing. Read More

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http://dx.doi.org/10.1016/j.nedt.2019.01.018DOI Listing
February 2019

Insight into bovine (Bos indicus) spermatozoal whole transcriptome profile.

Theriogenology 2019 Feb 12;129:8-13. Epub 2019 Feb 12.

Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, 388001, India. Electronic address:

Mature spermatozoa harbor both coding and non-coding type of RNAs which regulates spermatogenesis, fertilization and early development. Characterization of bovine sperm transcriptome can provide more insight into the molecular mechanisms involved in these processes. Here, we have analyzed whole transcriptome profile of Bos indicus spermatozoa to access the global RNA expression. Read More

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http://dx.doi.org/10.1016/j.theriogenology.2019.01.037DOI Listing
February 2019

Effects of miR-181a-5p abnormal expression on zebrafish (Danio rerio) vascular development following triclosan exposure.

Chemosphere 2019 Feb 11;223:523-535. Epub 2019 Feb 11.

Zhejiang Provincial Key Laboratory of Medical Genetics, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China. Electronic address:

Triclosan (TCS), one of the important bactericides, is widely used in personal care products, and its chronic exposure leads to severe toxic effects on the growth and development of blood vessels in zebrafish (Danio rerio). Herein, we screened out three differentially expressed miRNAs (miR-181a-5p, miR-132-3p and miR-128-3p) by sequencing and qRT-PCR analyses of 4-96-hpf TCS-exposed zebrafish, among which miR-181a-5p was found to regulate many signaling pathways involved in fatty acid biosynthesis and phosphatidylimositol signaling systems. By O-dianisidine staining, TCS-exposure resulted in decreased distribution of red blood cells and induced blood hypercoagulable state and thrombotic effects. Read More

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http://dx.doi.org/10.1016/j.chemosphere.2019.02.038DOI Listing
February 2019

Structure, dissolution, and plant uptake of ferrous/zinc phosphates.

Chemosphere 2019 Feb 12;223:310-318. Epub 2019 Feb 12.

National Nanotechnology Center, National Science and Technology Development Agency, 111 Thailand Science Park, Phahonyothin Road, Klong Luang, Pathum Thani, 12120, Thailand; AFRS(T), The Royal Society of Thailand, Sanam Sueapa, Dusit, Bangkok, 10300, Thailand. Electronic address:

Development of slow release fertilizers by tuning dissolution kinetics can reduce the environmental impact of (micro) nutrients added to crops. Mixed metal compounds may have different dissolution kinetics and plant uptake than single metal compounds. In this study, mixed Fe(II)/Zn(II) phosphates (0-100 at% Zn) were prepared by aqueous precipitation and their structural characteristics and dissolution kinetics in a sand column were measured as model for divalent metal and phosphate release in soil. Read More

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http://dx.doi.org/10.1016/j.chemosphere.2019.02.024DOI Listing
February 2019
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The elusive podocyte crossmatch for recurrent focal segmental glomerulosclerosis.

Kidney Int 2019 Mar;95(3):498-500

Peggy and Harold Katz Family Drug Discovery Center, University of Miami, Miller School of Medicine, Miami, Florida, USA; Katz Family Division of Nephrology and Hypertension, Department of Medicine, University of Miami, Miller School of Medicine, Miami, Florida, USA. Electronic address:

Focal segmental glomerulosclerosis is a glomerular disorder with a high rate of recurrence. Although kidney biopsies remain the gold standard to diagnose recurrent focal segmental glomerulosclerosis, non-invasive prognostic assays could facilitate preventive treatments and offer insight into disease pathogenicity and heterogeneity. Srivastava et al. Read More

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http://dx.doi.org/10.1016/j.kint.2018.11.032DOI Listing

Human FOXP3 Regulatory T Cell Heterogeneity and Function in Autoimmunity and Cancer.

Immunity 2019 Feb;50(2):302-316

Laboratory of Experimental Immunology, Immunology Frontier Research Center, Osaka University, Suita 565-0871, Japan; Department of Experimental Pathology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan. Electronic address:

Regulatory T (Treg) cells expressing the transcription factor Foxp3 have a critical role in the maintenance of immune homeostasis and prevention of autoimmunity. Recent advances in single cell analyses have revealed a range of Treg cell activation and differentiation states in different human pathologies. Here we review recent progress in the understanding of human Treg cell heterogeneity and function. Read More

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http://dx.doi.org/10.1016/j.immuni.2019.01.020DOI Listing
February 2019

The Secret Life of IgE-Producing Cells.

Immunity 2019 Feb;50(2):285-287

Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, New York University School of Medicine, New York, NY 10016, USA; Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA. Electronic address:

IgE antibodies are essential mediators of allergies. In a recent study in Science, Croote et al. (2018) characterize IgE cells isolated from individuals allergic to peanuts. Read More

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http://dx.doi.org/10.1016/j.immuni.2019.01.018DOI Listing
February 2019

Effect of crystalline structure on terbuthylazine degradation by HO-assisted TiO photocatalysis under visible irradiation.

J Environ Sci (China) 2019 May 13;79:153-160. Epub 2018 Dec 13.

Shenzhen Key Laboratory of Industrial Water Conservation and Municipal Wastewater Reclamation Technology, Shenzhen Polytechnic, Guangdong 518055, China.

Various methods for shifting the optical response of TiO into the visible (Vis) range have been reported. Herein, we reported the application of a TiO/HO/Vis process and the effects of TiO crystalline structure on the degradation of terbuthylazine. The results indicated that TiO crystalline structure and HO addition had significant effects on terbuthylazine degradation: its degradation rate could be increased from 7% to 70% with HO addition after 180 min of reaction, the synergistic degradation of terbuthylazine by TiO-Fe was substantially accelerated, with the degradation rate reaching up to 100% after 20 min of reaction, and rutile TiO showed better photocatalytic activity and a more obvious synergistic effect than anatase TiO. Read More

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http://dx.doi.org/10.1016/j.jes.2018.11.020DOI Listing

Digitization of healthcare organizations: The digital health landscape and information theory.

Int J Med Inform 2019 Apr 11;124:49-57. Epub 2019 Jan 11.

IMD Business School, Lausanne, Switzerland.

Background: This paper builds on an integrated framework of digital opportunities, based on information theory that was initially proposed by Claude Shannon: 1) providers of infrastructure for transmitting information; 2) platforms, devices, and software to bundle, store and deliver others' content; 3) original content creator); 4) platforms enabling peer-to-peer exchange; and 5) connected intelligence embed in devices. We aimed to clarify the meaning of digitization of organisations in the healthcare landscape and answer 'how can healthcare organisations compete in a world that is rapidly digitizing?'.

Methods: To gain more insight into the views of different stakeholders, we invited 12 participants with a wide range of backgrounds to a workshop. Read More

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http://dx.doi.org/10.1016/j.ijmedinf.2019.01.007DOI Listing

Bone Morphogenetic Protein-6 Attenuates Type 1 Diabetes Mellitus-Associated Bone Loss.

Stem Cells Transl Med 2019 Feb 19. Epub 2019 Feb 19.

Department of Orthopedics and Rehabilitation, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Patients with type 1 diabetes mellitus (T1DM) often suffer from osteopenia or osteoporosis. Although most agree that T1DM-induced hyperglycemia is a risk factor for progressive bone loss, the mechanisms for the link between T1DM and bone loss still remain elusive. In this study, we found that bone marrow-derived mesenchymal stem cells (BMSCs) isolated from T1DM donors were less inducible for osteogenesis than those from non-T1DM donors and further identified a mechanism involving bone morphogenetic protein-6 (BMP6) that was produced significantly less in BMSCs derived from T1DM donors than that in control cells. Read More

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http://dx.doi.org/10.1002/sctm.18-0150DOI Listing
February 2019

Fact-finding survey on diagnostic procedures and therapeutic interventions for parkinsonism accompanying dementia with Lewy bodies.

Psychogeriatrics 2019 Feb 19. Epub 2019 Feb 19.

Medical Affairs, Sumitomo Dainippon Pharma Co., Ltd., Tokyo, Japan.

Background: We performed a questionnaire survey of medical doctors engaged in the management of dementia to identify the actual status of treatment for dementia with Lewy bodies (DLB) in Japan.

Methods: Among participating medical doctors, we selected neurologists (Group N) and psychiatrists (Group P) because these physicians are usually involved in the management of DLB patients. The two groups were compared based on their diagnosis and treatment of DLB and in particular, parkinsonism. Read More

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http://dx.doi.org/10.1111/psyg.12408DOI Listing
February 2019

Bacterial community pattern along the sediment seafloor of the Arctic fjorden (Kongsfjorden, Svalbard).

Antonie Van Leeuwenhoek 2019 Feb 19. Epub 2019 Feb 19.

Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, People's Republic of China.

The Arctic region has been the focus of increasing attention as an ecosystem that is highly sensitive to changes associated with global warming. Although it was assumed to be vulnerable to changes in climate, a limited number of studies have been conducted on the surface sediment bacteria of Arctic fjorden. This study assessed the diversity and distribution pattern of bacterial communities in eight marine sediments along the seafloor in a high Arctic fjorden (Kongsfjorden, Svalbard). Read More

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http://dx.doi.org/10.1007/s10482-019-01245-zDOI Listing
February 2019

Whole Genome Sequence Analysis of Burkholderia contaminans FFH2055 Strain Reveals the Presence of Putative β-Lactamases.

Curr Microbiol 2019 Feb 19. Epub 2019 Feb 19.

Department of Biological Science, California State University Fullerton, 800 N State College Blvd, Fullerton, CA, 92831, USA.

Burkholderia contaminans is a member of the Burkholderia cepacia complex (Bcc), a pathogen with increasing prevalence among cystic fibrosis (CF) patients and the cause of numerous outbreaks due to the use of contaminated commercial products. The antibiotic resistance determinants, particularly β-lactamases, have been poorly studied in this species. In this work, we explored the whole genome sequence (WGS) of a B. Read More

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http://dx.doi.org/10.1007/s00284-019-01653-4DOI Listing
February 2019

A reference high-density genetic map of greater yam (Dioscorea alata L.).

Theor Appl Genet 2019 Feb 20. Epub 2019 Feb 20.

CIRAD, UMR AGAP, 97170, Petit-Bourg, Guadeloupe, France.

Key Message: This study generated the first high-density genetic map for D. alata based on genotyping-by-sequencing and provides new insight on sex determination in yam. Greater yam (Dioscorea alata L. Read More

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http://dx.doi.org/10.1007/s00122-019-03311-6DOI Listing
February 2019

Protein S-nitrosylation in programmed cell death in plants.

Cell Mol Life Sci 2019 Feb 19. Epub 2019 Feb 19.

College of Horticulture, Gansu Agricultural University, Lanzhou, People's Republic of China.

Programmed cell death (PCD) is associated with different phases of plant life and provides resistance to different kinds of biotic or abiotic stress. The redox molecule nitric oxide (NO) is usually produced during the stress response and exerts dual effects on PCD regulation. S-nitrosylation, which NO attaches to the cysteine thiol of proteins, is a vital posttranslational modification and is considered as an essential way for NO to regulate cellular redox signaling. Read More

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http://dx.doi.org/10.1007/s00018-019-03045-0DOI Listing
February 2019

Overcoming Barriers to Cervical Screening Among Pacific Women: A Narrative Review.

Health Equity 2019 14;3(1):22-29. Epub 2019 Feb 14.

Centre for Midwifery and Women's Health Research, Faculty of Health and Environmental Sciences, Auckland University of Technology, Auckland, New Zealand.

This narrative review explores the barriers and facilitators for Pacific women accessing the cervical screening pathway. Despite organized cervical screening in New Zealand, Pacific women still face significant health disparities in regard to cervical cancer incidence and mortality and access to colposcopy services. Providing a narrative synthesis of the available literature examining Pacific women and the barriers and facilitators to the cervical screening pathway may provide some insight into the provision of primary and secondary health services for Pacific women. Read More

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http://dx.doi.org/10.1089/heq.2018.0076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379043PMC
February 2019

DLA class II risk haplotypes for autoimmune diseases in the bearded collie offer insight to autoimmunity signatures across dog breeds.

Canine Genet Epidemiol 2019 15;6. Epub 2019 Feb 15.

1Department of Animal Science, University of California, One Shields Ave, Davis, CA 95616 USA.

Background: Primary hypoadrenocorticism (Addison's disease, AD) and symmetrical lupoid onychodystrophy (SLO) are two clinical conditions with an autoimmune etiology that occur in multiple dog breeds. In man, autoimmunity is associated with polymorphisms in immune-related genes that result in a reduced threshold for, or defective regulation of, T cell activation. The major histocompatibility complex (MHC) class II genes encode molecules that participate in these functions, and polymorphisms within these genes have been associated with autoimmune conditions in dogs and humans. Read More

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http://dx.doi.org/10.1186/s40575-019-0070-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6376674PMC
February 2019

Tissue Imaging by Mass Spectrometry: A Practical Guide for the Medicinal Chemist.

ACS Med Chem Lett 2019 Feb 11;10(2):161-167. Epub 2019 Jan 11.

Discovery Chemistry and Technology, AbbVie Inc., 1 North Waukegan Road, North Chicago, Illinois 60064, United States.

Understanding the tissue distribution of therapeutic molecules is often critical for assessing their efficacy and toxicity. Unfortunately, standard methods for monitoring localized drug distribution are resource-intensive and are typically performed late in the discovery process. As a result, early development efforts often progress without detailed information on the effect that changes in structure and/or formulation have on drug localization. Read More

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http://pubs.acs.org/doi/10.1021/acsmedchemlett.8b00480
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http://dx.doi.org/10.1021/acsmedchemlett.8b00480DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6378676PMC
February 2019
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Irradiation-Dependent Helium Gas Bubble Superlattice in Tungsten.

Sci Rep 2019 Feb 19;9(1):2277. Epub 2019 Feb 19.

Nuclear Science and Technology Department, Brookhaven National Laboratory, Upton, NY, 11973, United States.

The implantation of noble gas atoms into metals at high gas concentrations can lead to the self-organization of nanobubbles into superlattices with symmetry similar to the metal host matrix. Here, we examine the influence of implantation parameters on the formation and structure of helium gas bubble superlattices within a tungsten host matrix to uncover mechanistic insight into the formation process. The determination of the size and symmetry of the gas bubbles was performed using a combination of small angle x-ray scattering and transmission electron microscopy. Read More

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http://dx.doi.org/10.1038/s41598-019-39053-0DOI Listing
February 2019

Compressing the lumbar nerve root changes the frequency-associated cerebral amplitude of fluctuations in patients with low back/leg pain.

Sci Rep 2019 Feb 19;9(1):2246. Epub 2019 Feb 19.

Department of Radiology, The First Affiliated Hospital, Nanchang University, Nanchang, 330006, People's Republic of China.

Understanding the central mechanisms responsible for lumbar nerve root compression may facilitate the development of new therapeutic strategies. In this study, our aim was to investigate the amplitude of fluctuations (AF) in five specific frequency bands and the full-frequency band realm to provide novel insight into the rhythm of the neuronal activity of low back/leg pain (LBLP) patients (n = 25). Compared with healthy controls, LBLP patients exhibited a significantly altered AF in multiple brain regions, including the right or left middle and inferior temporal gyri, bilateral precuneus, right anterior insula/frontal operculum, right or left inferior parietal lobule/postcentral gyrus, and other locations at five specific frequencies (P < 0. Read More

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http://dx.doi.org/10.1038/s41598-019-38721-5DOI Listing
February 2019

Transcriptome-derived investigation of biosynthesis of quinolizidine alkaloids in narrow-leafed lupin (Lupinus angustifolius L.) highlights candidate genes linked to iucundus locus.

Sci Rep 2019 Feb 19;9(1):2231. Epub 2019 Feb 19.

Department of Genomics, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznań, Poland.

Unravelling the biosynthetic pathway of quinolizidine alkaloids (QAs), regarded as antinutritional compounds of narrow-leafed lupin (NLL) seeds, is fundamental to best exploit NLL as food or feed. We investigated 12 candidate genes connected to QA biosynthesis, selecting them by transcriptomic and genomic approaches, from the landscape of genes differentially expressed in leaves of the high- and low-alkaloid NLL accessions. Linkage analysis enabled the assessment of the location of the candidate genes in relation to iucundus, a major locus of unknown identity, that confers reduced QA content in seeds. Read More

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http://dx.doi.org/10.1038/s41598-018-37701-5DOI Listing
February 2019

Insight of Captagon Abuse by Chemogenomics Knowledgebase-guided Systems Pharmacology Target Mapping Analyses.

Sci Rep 2019 Feb 19;9(1):2268. Epub 2019 Feb 19.

Department of Pharmaceutical Sciences and Computational Chemical Genomics Screening Center, School of Pharmacy, University of Pittsburgh, Pittsburgh, Pennsylvania, 15261, United States.

Captagon, known by its genetic name Fenethylline, is an addictive drug that complicates the War on Drugs. Captagon has a strong CNS stimulating effect than its primary metabolite, Amphetamine. However, multi-targets issues associated with the drug and metabolites as well as its underlying mechanisms have not been fully defined. Read More

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http://dx.doi.org/10.1038/s41598-018-35449-6DOI Listing
February 2019

Cysteine-rich granulin-3 rapidly promotes amyloid-β fibrils in both redox states.

Biochem J 2019 Feb 19. Epub 2019 Feb 19.

University of Southern Mississippi, 118 College Dr # 5043, Hattiesburg, Hattiesburg, Mississippi, 39406, United States

Granulins (GRNs 1 - 7) are cysteine-rich proteolytic products of progranulin (PGRN) that have recently been implicated in neurodegenerative diseases including frontotemporal dementia (FTD) and Alzheimer disease (AD). Their precise mechanism in these pathologies remains uncertain, but both inflammatory and lysosomal roles have been observed for GRNs. Among the seven GRNs, GRN‑3 is well-characterized and is implicated within the context of FTD. Read More

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http://dx.doi.org/10.1042/BCJ20180916DOI Listing
February 2019

Neuropsychiatric Genetics of African Populations-Psychosis (NeuroGAP-Psychosis): a case-control study protocol and GWAS in Ethiopia, Kenya, South Africa and Uganda.

BMJ Open 2019 Feb 19;9(2):e025469. Epub 2019 Feb 19.

Department of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA.

Introduction: Schizophrenia and bipolar disorder account for a large proportion of the global burden of disease. Despite their enormous impact, little is known about their pathophysiology. Given the high heritability of schizophrenia and bipolar disorder, unbiased genetic studies offer the opportunity to gain insight into their neurobiology. Read More

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http://dx.doi.org/10.1136/bmjopen-2018-025469DOI Listing
February 2019

Innate lymphoid cell type 3-derived interleukin-22 boosts lipocalin-2 production in intestinal epithelial cells via synergy between STAT3 and NF-kB.

J Biol Chem 2019 Feb 19. Epub 2019 Feb 19.

Laboratory Medicine (LABMED), Karolinska Institutet, Sweden.

Escherichia coli and Klebsiella pneumoniae are opportunistic pathogens that are commonly associated with infections at mucosal surfaces, such as the lung or the gut. The host response against these types of infections includes the release of epithelial-derived antimicrobial factors such as lipocalin-2 (LCN-2), a protein which specifically inhibits the iron-acquisition of Enterobacteriaceae by binding and neutralizing the bacterial iron-scavenging molecule enterobactin. Regulation of epithelial antimicrobial responses, including the release of LCN-2, has previously been shown to depend on IL-22, a cytokine produced by innate lymphoid cells type 3 (ILC3) during Enterobacteriaceae infections. Read More

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http://dx.doi.org/10.1074/jbc.RA118.007290DOI Listing
February 2019

Novel tool to quantify cell wall porosity relates wall structure to cell growth and drug uptake.

J Cell Biol 2019 Feb 19. Epub 2019 Feb 19.

College of Life Sciences, Northwest A&F University, Yangling, China

Even though cell walls have essential functions for bacteria, fungi, and plants, tools to investigate their dynamic structure in living cells have been missing. Here, it is shown that changes in the intensity of the plasma membrane dye FM4-64 in response to extracellular quenchers depend on the nano-scale porosity of cell walls. The correlation of quenching efficiency and cell wall porosity is supported by tests on various cell types, application of differently sized quenchers, and comparison of results with confocal, electron, and atomic force microscopy images. Read More

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http://dx.doi.org/10.1083/jcb.201810121DOI Listing
February 2019

Qualitative study to elicit patients' and primary care physicians' perspectives on the use of a self-management mobile health application for knee osteoarthritis.

BMJ Open 2019 Feb 1;9(1):e024016. Epub 2019 Feb 1.

Department of Community Health Sciences, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada.

Objective: To elicit perspectives of family physicians and patients with knee osteoarthritis (KOA) on KOA, its treatment/management and the use of a mobile health application (app) to help patients self-manage their KOA.

Design: A qualitative study using Cognitive Task Analysis for physician interviews and peer-to-peer semistructured interviews for patients according to the Patient and Community Engagement Research (PaCER) method.

Setting: Primary care practices and patient researchers at an academic centre in Southern Alberta. Read More

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http://dx.doi.org/10.1136/bmjopen-2018-024016DOI Listing
February 2019

A Two-Pulse Cellular Stimulation Test Elucidates Variability and Mechanisms in Signaling Pathways.

Biophys J 2019 Jan 30. Epub 2019 Jan 30.

Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan; Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan. Electronic address:

Mammalian cells respond in a variable manner when provided with physiological pulses of ligand, such as low concentrations of acetylcholine present for just tens of seconds or TNFα for just tens of minutes. For a two-pulse stimulation, some cells respond to both pulses, some do not respond, and yet others respond to only one or the other pulse. Are these different response patterns the result of the small number of ligands being able to only stochastically activate the pathway at random times or an output pattern from a deterministic algorithm responding differently to different stimulation intervals? If the response is deterministic in nature, what parameters determine whether a response is generated or skipped? To answer these questions, we developed a two-pulse test that utilizes different rest periods between stimulation pulses. Read More

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http://dx.doi.org/10.1016/j.bpj.2019.01.022DOI Listing
January 2019

Smartphone-based fluorescent lateral flow immunoassay platform for highly sensitive point-of-care detection of Zika virus nonstructural protein 1.

Anal Chim Acta 2019 May 23;1055:140-147. Epub 2018 Dec 23.

Beijing Institute of Radiation Medicine, Beijing, 100850, PR China; Beijing Key Laboratory of New Molecular Diagnosis Technologies for Infectious Diseases, Beijing, 100850, PR China. Electronic address:

Simple, inexpensive, and rapid diagnostic tests in low-resource settings with limited laboratory equipment and technical expertise are instrumental in reducing morbidity and mortality from epidemic infectious diseases. We developed a smartphone-based fluorescent lateral flow immunoassay (LFIA) platform for the highly sensitive point-of-care detection of Zika virus nonstructural protein 1 (ZIKV NS1). An attachment was designed and 3D-printed to integrate the smartphone with external optical and electrical components, enabling the miniaturization of the instrument and reduction in cost and complexity. Read More

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http://dx.doi.org/10.1016/j.aca.2018.12.043DOI Listing

"Confidence comes with frequent practice": health professionals' perceptions of using manual vacuum aspiration after a training program.

Reprod Health 2019 Feb 19;16(1):20. Epub 2019 Feb 19.

Department of Public Health and Nursing, Norwegian University of Science and Technology, Trondheim, Norway.

Background: Malawi has one of the highest maternal mortality rates in the world, with unsafe abortion as a major contributor. Curettage is most frequently used as the surgical method for treating incomplete abortions, even though it is costly for an impoverished health system and the less expensive and safe manual vacuum aspiration (MVA) method is recommended.

Methods: The aim of this 2016-17 study is to explore health worker's perception of doing MVA 1 year after an educational intervention. Read More

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http://dx.doi.org/10.1186/s12978-019-0683-zDOI Listing
February 2019

Prediction of lncRNA-disease associations by integrating diverse heterogeneous information sources with RWR algorithm and positive pointwise mutual information.

BMC Bioinformatics 2019 Feb 19;20(1):87. Epub 2019 Feb 19.

Department of Biomedical Informatics, University of Pittsburgh, 5607 Baum Blvd, Pittsburgh, PA, 15206, USA.

Background: Long non-coding RNAs play an important role in human complex diseases. Identification of lncRNA-disease associations will gain insight into disease-related lncRNAs and benefit disease diagnoses and treatment. However, using experiments to explore the lncRNA-disease associations is expensive and time consuming. Read More

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http://dx.doi.org/10.1186/s12859-019-2675-yDOI Listing
February 2019

Experiential Palliative Care Immersion: Student Nurse's Narratives Reflect Care Competencies.

West J Nurs Res 2019 Feb 19:193945919833061. Epub 2019 Feb 19.

2 University of Florida, Gainesville, FL, USA.

Many nurses report a lack of confidence providing care for patients facing a life-threatening illness. Palliative care leaders have devised primary palliative nursing care competencies (CARES [Competencies And Recommendations for Educating undergraduate nursing Students]) that all students should achieve. In this study, nursing students participated in an innovative palliative care immersion experience, the Comfort Shawl Project. Read More

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http://dx.doi.org/10.1177/0193945919833061DOI Listing
February 2019