179,325 results match your criteria INSIGHT Newspaper [Journal]


2018 Infectious Diseases Society of America Clinical Practice Guideline for the Management of Outpatient Parenteral Antimicrobial Therapy.

Clin Infect Dis 2019 Jan;68(1):1-4

Department of Medicine, Perelman School of Medicine, University of Pennsylvania, and Center for Evidence-based Practice, University of Pennsylvania Health System, Philadelphia.

A panel of experts was convened by the Infectious Diseases Society of America to update the 2004 clinical practice guideline on outpatient parenteral antimicrobial therapy (OPAT) [1]. This guideline is intended to provide insight for healthcare professionals who prescribe and oversee the provision of OPAT. It considers various patient features, infusion catheter issues, monitoring questions, and antimicrobial stewardship concerns. Read More

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January 2019

Immunosuppression, oxidative stress, and glycometabolism disorder caused by cadmium in common carp (Cyprinus carpio L.): Application of transcriptome analysis in risk assessment of environmental contaminant cadmium.

J Hazard Mater 2018 Dec 5;366:386-394. Epub 2018 Dec 5.

College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, PR China. Electronic address:

Cadmium (Cd), a hazardous environmental contaminant with irreversible toxicity to fish, has been detected in aquatic environment of many countries. The common carp is one of the most widely distributed fish in the world, so we used common carp to assess environmental contaminant risk. In present study, we investigated effects of Cd on immune function, oxidative defense, and glycometabolism in the spleens of common carp by transcriptome analysis. Read More

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December 2018

Influence of drivers' psychological risk profiles on the effectiveness of traffic calming measures.

Accid Anal Prev 2018 Dec 11;123:243-255. Epub 2018 Dec 11.

Department of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa, Via Paradisa 2, 56126, Pisa, Italy.

Road traffic injuries represent a serious public health problem and are one of leading cause of death, injury and disability around the world. Road accidents are often caused by an accumulation of factors; however, drivers appear to be by far the most decisive one. The driver's behaviour is complex and depends on reflex (or involuntary) and voluntary driving actions. Read More

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December 2018

Comprehensive characterization of in vivo metabolic profile of Polygalae radix based on ultra-high-performance liquid chromatography-tandem mass spectrometry.

J Pharm Biomed Anal 2018 Dec 5;165:173-181. Epub 2018 Dec 5.

National Center of Mass Spectrometry in Changchun and Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry and Chemical Biology Laboratory, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China; University of Science and Technology of China, Hefei, 230026, China. Electronic address:

In this study, a novel analysis strategy for progressively targeted screening and characterization of drug ingredients from in vitro to in vivo was proposed based on ultra-high performance liquid chromatography-tandem mass spectrometry for comprehensive characterization of in vivo metabolic profile of Polygalae radix (PR). First, an in vitro chemical profile of PR was described with the assistance of UNIFI™ software. The characteristic neutral small molecule losses were summarized to distinguish different chemical structures in the PR extract. Read More

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December 2018

Signal enhanced proteomics: a biological perspective on dissecting the functional organisation of cell proteomes.

Curr Opin Chem Biol 2018 Dec 11;48:114-122. Epub 2018 Dec 11.

Laboratory of Quantitative Proteomics, Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK. Electronic address:

Proteomes are highly dynamic and can respond rapidly to environmental and cellular signals. Within cells, proteins often form distinct pools with different functions and properties. However, in quantitative proteomics studies it is common to measure averaged values for proteins that do not reflect variations that may occur between different protein isoforms, different subcellular compartments, or in cells at different cell cycle stages and so on. Read More

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December 2018

Effects of hypoxia-reoxygenation stress on mitochondrial proteome and bioenergetics of the hypoxia-tolerant marine bivalve Crassostrea gigas.

J Proteomics 2018 Dec 11. Epub 2018 Dec 11.

Department of Marine Biology, University of Rostock, Rostock, Germany; Department of Maritime Systems, Interdisciplinary Faculty, University of Rostock, Rostock, Germany.

Mitochondria are key intracellular targets of hypoxia-reoxygenation (H/R) stress due to their central role in generation of ATP and reactive oxygen species (ROS). Intertidal oysters Crassostrea gigas are adapted to frequent H/R cycles and maintain aerobic function despite frequent oxygen fluctuations. To gain insight into the molecular mechanisms of H/R tolerance, we assessed changes in mitochondrial respiration and (phospho)proteome of C. Read More

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December 2018

Dissecting the Thermodynamic Contributions of the Charged Residues in the Membrane Anchoring of Bcl-xl C-Terminal Domain.

Chem Phys Lipids 2018 Dec 11. Epub 2018 Dec 11.

Bioinformatics Centre, Bose Institute, P-1/12 C.I.T. Scheme VII M, Kolkata, 700054, India. Electronic address:

The C-terminal helix of the Bcl-xl is known to initiate the membrane insertion of the protein by anchoring into the mitochondrial outer membrane. The C-terminal charged residues of that helix, R232, and K233, are reported to have an important structural role in the process of insertion. The present work provides a quantitative understanding of the thermodynamic contribution of these residues on the membrane insertion profile from Adaptive Biasing Force based MD simulations of 2. Read More

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December 2018

Biochemical characterization of a native group III trypsin ZT from Atlantic cod (Gadus morhua).

Int J Biol Macromol 2018 Dec 11. Epub 2018 Dec 11.

Faculty of Food Science and Nutrition, School of Health Sciences, University of Iceland. University of Iceland, Sæmundargata 2, 101 Reykjavik, Iceland. Electronic address:

Atlantic cod trypsin ZT is biochemically characterized for the first time in this report in comparison to a group I trypsin (cod trypsin I). To our knowledge, trypsin ZT is the first thoroughly characterized group III trypsin. A more detailed understanding of trypsin ZT biochemistry may give insight into its physiological role as well as its potential use within the biotechnology sector. Read More

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December 2018

Novel aspects of PCSK9 and lipoprotein receptors in renal disease-related dyslipidemia.

Cell Signal 2018 Dec 11. Epub 2018 Dec 11.

Dept. of Nephrology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.

Chronic kidney disease (CKD) is a global health problem with a profound impact on quality of life. Cardiovascular disease is established as a major cause of morbidity and mortality in patients with CKD. Dyslipidemia is frequently observed in CKD patients, suggesting a causal relation between dyslipidemia and cardiovascular disease in CKD patients. Read More

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December 2018

An optimized protocol for the analysis of house dust mite (Dermatophagoides farinae)-induced neutrophil-dominant airway inflammation.

J Immunol Methods 2018 Dec 11. Epub 2018 Dec 11.

Department of Immunology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan; AMED-CREST, AMED, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan. Electronic address:

House dust mites (HDMs), Dermatophagoides sp., are one of the most widespread aeroallergens worldwide and cause various allergic diseases, including asthma. The pathophysiology of asthma has been intensively investigated using murine models of allergic airway inflammation induced by exposure to D. Read More

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December 2018

Identification and immune-related analysis of SNPs in Litopenaeus vannamei Toll3 receptor.

Immunol Lett 2018 Dec 11. Epub 2018 Dec 11.

Department of Biology and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, 515063, China. Electronic address:

Tolls and Toll-like receptors (TLRs), as innate immune-recognition receptors that recognize molecular patterns associated with microbial pathogens, play a critical role in antimicrobial immune responses. Here, we report on single nucleotide polymorphisms (SNPs) of Litopenaeus vannamei Toll3 (LvToll3). Multiple sequence alignment of the L. Read More

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December 2018

Structural and functional insight into serine hydroxymethyltransferase from Helicobacter pylori.

PLoS One 2018 14;13(12):e0208850. Epub 2018 Dec 14.

Laboratory of Optics and Biosciences, Ecole polytechnique, CNRS, INSERM, Université Paris Saclay, Palaiseau, France.

Serine hydroxymethyltransferase (SHMT), encoded by the glyA gene, is a ubiquitous pyridoxal 5'-phosphate (PLP)-dependent enzyme that catalyzes the formation of glycine from serine. The thereby generated 5,10-methylene tetrahydrofolate (MTHF) is a major source of cellular one-carbon units and a key intermediate in thymidylate biosynthesis. While in virtually all eukaryotic and many bacterial systems thymidylate synthase ThyA, SHMT and dihydrofolate reductase (DHFR) are part of the thymidylate/folate cycle, the situation is different in organisms using flavin-dependent thymidylate synthase ThyX. Read More

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December 2018

CD71+VISTA+ erythroid cells promote the development and function of regulatory T cells through TGF-β.

PLoS Biol 2018 Dec 14;16(12):e2006649. Epub 2018 Dec 14.

Department of Dentistry, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Canada.

Cell-surface transferrin receptor (CD71+) erythroid cells are abundant in newborns with immunomodulatory properties. Here, we show that neonatal CD71+ erythroid cells express significant levels of V-domain Immunoglobulin (Ig) Suppressor of T Cell Activation (VISTA) and, via constitutive production of transforming growth factor (TGF)- β, play a pivotal role in promotion of naïve CD4+ T cells into regulatory T cells (Tregs). Interestingly, we discovered that CD71+VISTA+ erythroid cells produce significantly higher levels of TGF-β compared to CD71+VISTA- erythroid cells and CD71+ erythroid cells from the VISTA knock-out (KO) mice. Read More

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December 2018

Augmented ILT3/LILRB4 Expression of Peripheral Blood Antibody Secreting Cells in the Acute-Phase of Kawasaki Disease.

Pediatr Infect Dis J 2018 Dec 13. Epub 2018 Dec 13.

Department of Pediatrics, Sendai Medical Center, 2-8-8 Miyagino, Sendai 983-8520, Japan.

Background: Kawasaki disease (KD) is an acute, systemic vasculitis syndrome that occurs in children. The clinical symptoms and epidemiological features of KD strongly suggest that KD is triggered by unidentified infectious agents in genetically predisposed patients. In addition, a number of studies have described the role of B cells in the development of KD. Read More

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December 2018

Communicating Risks of Adjuvant Chemotherapy for Breast Cancer: Getting Beyond the Laundry List.

J Oncol Pract 2018 Dec 14:JOP1800162. Epub 2018 Dec 14.

UCLA Jonsson Comprehensive Cancer Center; UCLA Anderson School of Management; UCLA Schools of Medicine and Public Health, Los Angeles; UC Davis Center for Healthcare Policy and Research, Davis, CA; and University of Texas Health Sciences Center, San Antonio, TX.

Purpose:: According to the Institute of Medicine, high-quality cancer care should include effective communication between clinicians and patients about the risks and benefits, expected response, and impact on quality of life of a recommended therapy. In the delivery of oncology care, the barriers to and facilitators of communication about potential long-term and late effects, post-treatment expectations, and transition to survivorship care have not been fully defined.

Patients And Methods:: We collected qualitative data through semistructured interviews with medical oncologists and focus groups with breast cancer survivors and applied the Theoretical Domains Framework to systematically analyze and identify the factors that may influence oncologists' communication with patients with breast cancer about the long-term and late effects of adjuvant therapy. Read More

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December 2018

[Research on immune regulation mechanism of immunotherapy for allergic rhinitis].

Authors:
Y L Jiang X H Zhu

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2018 Sep;32(18):1440-1443

Immunotherapy, among all the treatments of allergic rhinitis, is the only one which aims directly at etiological factor.Even though, subcutaneous immunotherapy is a widely used and effective therapeutic method in clinical practice, there is no definite answer in the current research about how it regulates the immune mechanism of allergic rhinitis, that is, how to predict the curative effect according to its objective indicators. In order to have an insight into its clinical role in the immune mechanism of allergic rhinitis,this article will have an overview about it, on the basis of the pertinent medical literature. Read More

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September 2018

Early Placebo Improvement Is a Marker for Subsequent Placebo Response in Long-Acting Injectable Antipsychotic Trials for Schizophrenia: Combined Analysis of 4 RCTs.

J Clin Psychiatry 2018 Dec 4;80(1). Epub 2018 Dec 4.

Department of Neuropsychiatry, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.

Objective: Placebo effects remain largely unexplored in clinical trials of long-acting injectable (LAI) antipsychotics for schizophrenia. This study aims to characterize patients showing improvements after placebo injections and to search for criteria for the prediction of subsequent response based on the magnitude of score changes after the first week of treatment.

Methods: Data from 450 patients with schizophrenia (DSM-IV) who received placebo injections in 4 double-blind randomized controlled trials evaluating efficacy of LAI paliperidone palmitate obtained through the Yale University Open Data Access (YODA) project were analyzed. Read More

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December 2018

Patients' narratives of their patient participation in the myocardial infarction pathway.

J Adv Nurs 2018 Dec 14. Epub 2018 Dec 14.

Clinic of medicine and rehabilitation, Møre og Romsdal Hospital Trust, Ålesund, Norway.

Aim: To explore how patients in areas without local percutaneous coronary intervention facilities experience patient participation in different phases of the myocardial infarction pathway.

Background: Acute treatment of myocardial infarction often involves percutaneous coronary intervention. In Norway, this treatment is centralized at certain hospitals; thus, patients often require long-distance transportation and experience frequent hospital transfers. Read More

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December 2018

Capsular polysaccharide of Streptococcus agalactiae is an essential virulence factor for infection in Nile tilapia (Oreochromis niloticus Linn.).

J Fish Dis 2018 Dec 14. Epub 2018 Dec 14.

Core Facility for Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.

Streptococcus agalactiae (Group B Streptococcus, GBS) is associated with diverse diseases in aquatic animals. The capsule polysaccharide (CPS) encoded by the cps gene cluster is the major virulence factor of S. agalactiae; however, limited information is available regarding the pathogenic role of the CPS of serotype Ia piscine GBS strains in fish. Read More

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December 2018

Biallelic CCM3 mutations cause a clonogenic survival advantage and endothelial cell stiffening.

J Cell Mol Med 2018 Dec 13. Epub 2018 Dec 13.

Department of Human Genetics, University Medicine Greifswald and Interfaculty Institute for Genetics and Functional Genomics, University of Greifswald, Greifswald, Germany.

CCM3, originally described as PDCD10, regulates blood-brain barrier integrity and vascular maturation in vivo. CCM3 loss-of-function variants predispose to cerebral cavernous malformations (CCM). Using CRISPR/Cas9 genome editing, we here present a model which mimics complete CCM3 inactivation in cavernous endothelial cells (ECs) of heterozygous mutation carriers. Read More

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December 2018

Proteomic Evidence of Biological Ageing in a Child with a Compound Heterozygous ZMPSTE24 Mutation.

Proteomics Clin Appl 2018 Dec 13:e1800135. Epub 2018 Dec 13.

A. K. Lucas-Herald, B. Mansoorian, W. Mullen, C. Delles, Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, G12 8TA, UK.

Background: Progeria-like syndromes offer a unique insight into ageing. Here we present the case of a boy affected with mandibuloacral dysplasia and compound heterozygous mutations in ZMPSTE24.

Methods: Capillary electrophoresis-mass spectroscopy was used for proteome analysis to analyse peptides previously found to be differentially regulated in chronic kidney disease (273 peptides defining the CKD273 classifier), coronary artery disease (238 peptides defining the CAD238 classifier) and ageing (116 peptides defining the AGE116 classifier). Read More

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December 2018

Extracellular Surface Potential Mapping by Scanning Ion Conductance Microscopy Revealed Transient Transmembrane Pore Formation Induced by Conjugated Polymer Nanoparticles.

Macromol Biosci 2018 Dec 13:e1800271. Epub 2018 Dec 13.

Department of Physics, Biomolecular Sciences Institute, Florida International University, FL, 33199, USA.

In-depth understanding of the biophysicochemical interactions at the nano-bio interface is important for basic cell biology and applications in nanomedicine and nanobiosensors. Here, the extracellular surface potential and topography changes of live cell membranes interacting with polymeric nanomaterials using a scanning ion conductance microscopy-based potential imaging technique are investigated. Two structurally similar amphiphilic conjugated polymer nanoparticles (CPNs) containing different functional groups (i. Read More

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December 2018

Dissociation in awareness of memory and language decline in Alzheimer's disease.

Int J Geriatr Psychiatry 2018 Dec 12. Epub 2018 Dec 12.

Center for Memory and Attention Disorders, Neurological Institute, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel.

Objectives: The aim of this study was to examine awareness of decline in memory and in language in individuals with Alzheimer's disease (AD), by comparing participant and informant ratings, as well as these ratings and actual test performance.

Methods: We analyzed data from 149 individuals with AD enrolled in the Alzheimer's Disease Neuroimaging Initiative (ADNI) who filled the Everyday Cognition questionnaire, and performed memory and language tasks.

Results: Participants provided significantly lower assessments of decline than did informants for both memory and language. Read More

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December 2018

MicroRNA-384 regulates cell proliferation and apoptosis through directly targeting WISP1 in laryngeal cancer.

J Cell Biochem 2018 Dec 12. Epub 2018 Dec 12.

Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Laryngeal cancer (LC) is an increasingly common malignant tumors of head and neck cancer. Aberrant expression of microRNA (miRNA) is closely related with LC development. In the current study, we investigated the biological function and underlying molecular mechanism of miR-384 in LC. Read More

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December 2018

Romantic Conflict Narratives in Emerging Adult Couples: Viewpoint and Gender Matter.

Fam Process 2018 Dec 13. Epub 2018 Dec 13.

Merrill Palmer Skillman Institute, Wayne State University, Detroit, MI.

Healthy romantic relationships entail understanding the needs of the self and other when interpreting conflict events. Yet how couples make meaning around specific conflicts and their capacity to reflect on their own and their partners' unmet needs is understudied. Using narratives, we examined destructive (e. Read More

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December 2018

Enhanced stability and optical absorption in the perovskite-based compounds MA$_{1-x}$Cs$_x$PbI$_{3-y}$Br$_y$.

Chemphyschem 2018 Dec 12. Epub 2018 Dec 12.

Southwest University, School of Physical Science and Technology, Tian Sheng Lu 2#, 400715, Chongqing, CHINA.

Organometal halide perovskites have been outstanding from enormous amount of functional materials thanks to their highly cost-effective processability and prominent light harvesting capacity. Unfortunately, poor long-term stability seriously hinders their further development. The recent experimental observations suggest that Cesium is a promising candidate to enhance the stability of MAPbI$_3$. Read More

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December 2018

Eosin Y-Functionalized Conjugated Organic Polymers for Visible-Light-Driven CO Reduction with H O to CO with High Efficiency.

Angew Chem Int Ed Engl 2018 Nov 22. Epub 2018 Nov 22.

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Colloid, Interface and Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.

Visible-light-driven photoreduction of CO to energy-rich chemicals in the presence of H O without any sacrifice reagent is of significance, but challenging. Herein, Eosin Y-functionalized porous polymers (PEosinY-N, N=1-3), with high surface areas up to 610 m  g , are reported. They exhibit high activity for the photocatalytic reduction of CO to CO in the presence of gaseous H O, without any photosensitizer or sacrifice reagent, and under visible-light irradiation. Read More

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November 2018

High- and Ultra-High definition of IR spectral histopathology gives an insight into chemical environment of lung metastases in breast cancer.

J Biophotonics 2018 Dec 12:e201800345. Epub 2018 Dec 12.

Faculty of Chemistry, Jagiellonian University, Poland.

Using high- (HD) and ultra-high-definition (UHD) of Fourier Transform Infrared (FTIR) spectroscopic imaging, we characterised spectrally pulmonary metastases in a murine model of breast cancer comparing them with histopathological results (H&E staining). This comparison showed excellent agreement between the methods in case of localisation of metastases with size below 1 mm and revealed that label-free HD and UHD IR spectral histopathology distinguish the type of neoplastic cells. We primary focused on differentiation between metastatic foci in the pleural cavity from cancer cells present in lung parenchyma and inflamed cells present in extracellular matrix of lungs due to growing of advanced metastases. Read More

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December 2018

A qualitative evaluation of the effects of Metacognitive Reflection and Insight Therapy: 'Living more consciously'.

Psychol Psychother 2018 Dec 12. Epub 2018 Dec 12.

Department of Clinical Psychology and Experimental Psychopathology, Faculty of Behavioral Sciences, University of Groningen, The Netherlands.

Objectives: Extensive research showed that one of the major difficulties that people with schizophrenia spectrum disorders are struggling with involves their ability to reflect on their own and others' mental activities, also defined as metacognition. Several new psychotherapies have been developed to assist patients (re)gain metacognitive capacity, including Metacognitive Reflection and Insight Therapy (MERIT). The current study investigated the client's subjective experience of psychotherapy, to determine whether service users found MERIT effective and whether these gains align with quantitative findings, which processes they considered responsible for these benefits, in which ways participants found MERIT similar or different from other interventions, and whether they experienced non-desirable factors and outcomes. Read More

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December 2018

Patterns of mercury and selenium exposure in Minnesota common loons.

Environ Toxicol Chem 2018 Dec 11. Epub 2018 Dec 11.

Minnesota Department of Natural Resources, 500 Lafayette Road, St. Paul, Minnesota 55155, USA.

Common loons (Gavia immer) are at risk of elevated dietary mercury (Hg) exposure in portions of their breeding range. To assess the level of risk among loons in Minnesota, we investigated loon blood Hg concentrations in breeding lakes across Minnesota. Loon blood Hg concentrations were regressed on predicted Hg concentrations in standardized 12-cm whole organism yellow perch (Perca flavescens), based on fish Hg records from Minnesota lakes, using the U. Read More

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December 2018

JAK2 regulates mismatch repair protein-mediated epigenetic alterations in response to oxidative damage.

Environ Mol Mutagen 2018 Dec 11. Epub 2018 Dec 11.

Medical Sciences, Indiana University School of Medicine, Bloomington, Indiana, United States of America, 47405.

At sites of chronic inflammation epithelial cells undergo aberrant DNA methylation that contributes to tumorigenesis. Inflammation is associated with an increase in reactive oxygen species (ROS) that cause oxidative DNA damage, which has also been linked to epigenetic alterations. We previously demonstrated that in response to ROS, mismatch repair (MMR) proteins MSH2 and MSH6 recruit epigenetic silencing proteins DNA methyltransferase 1 (DNMT1) and Polycomb repressive complex 2 (PRC2) members to sites of DNA damage, resulting in transcriptional repression of tumor suppressor genes (TSGs). Read More

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December 2018

TIGD1, a gene of unknown function, involves cell-cycle progression and correlates with poor prognosis in human cancer.

J Cell Biochem 2018 Dec 11. Epub 2018 Dec 11.

Laboratory of Tropical Biomedicine and Biotechnology, School of Tropical Medicine and Laboratory Medicine, Hainan Medical University, Haikou, China.

Background: Trigger transposable element-derived 1 (TIGD1), a human species-specific gene, was identified as a cell cycle-related differentially expressed gene during the formation of liver cancer. As far as we know, there are no reports about the function of TIGD1. This study aimed to explore the expression of TIGD1 in human cancers and establish whether elevated TIGD1 can be used as a prognostic cancer biomarker and its potential role in cancer. Read More

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December 2018

MicroRNA-7b attenuates ischemia/reperfusion-induced H9C2 cardiomyocyte apoptosis via the hypoxia inducible factor-1/p-p38 pathway.

J Cell Biochem 2018 Dec 11. Epub 2018 Dec 11.

Department of Cardiology, Zhongda Hospital, Medical School of Southeast University, Nanjing, China.

Objective: MicroRNAs (miRNAs) have been shown to play crucial roles in the occurrence, development, and treatment of many cardiovascular diseases. Coronary heart disease (CAD)-related miRNAs are still a growing research area. miR-7b was reported to be downregulated in acute myocardial infarction (AMI) myocardium tissues. Read More

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December 2018

Three-dimensional analysis reveals altered chromatin interaction by enhancer inhibitors harbors TCF7L2-regulated cancer gene signature.

J Cell Biochem 2018 Dec 11. Epub 2018 Dec 11.

Biomedical Health Sciences Department, University of Vermont, Burlington, VT, USA.

Distal regulatory elements influence the activity of gene promoters through chromatin looping. Chromosome conformation capture (3C) methods permit identification of chromatin contacts across different regions of the genome. However, due to limitations in the resolution of these methods, the detection of functional chromatin interactions remains a challenge. Read More

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December 2018

Increased circulating microparticles in streptozotocin-induced diabetes propagate inflammation contributing to microvascular dysfunction.

J Physiol 2018 Dec 11. Epub 2018 Dec 11.

Department of Cellular and Molecular Physiology, College of Medicine, Penn State University, Hershey, PA, 17033.

In various cardiovascular diseases, microparticles (MPs), the membrane-derived vesicles released during cell activation, are markedly increased in the circulation. These MPs have been recognized to play diverse roles in the regulation of cellular functions. However, current knowledge of MP function has been largely concluded from in vitro studies. Read More

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December 2018

Covalent Vs Non-Covalent inhibition: Tackling Drug Resistance in EGFR - A Thorough Dynamic Perspective.

Chem Biodivers 2018 Dec 11. Epub 2018 Dec 11.

University of KwaZulu-Natal, Westville, School of Health Sciences, School of Health Sciences, University of, Not Available, 4001, Durban, SOUTH AFRICA.

A persistent challenge in the treatment of non-small cell lung cancer (NSCLC) with EGFR is the emergence of drug-resistant caused by somatic mutations. The EGFR L858R/T790M double mutant (EGFRDM) was found to be the most alarming variant. Despite the development of a wide range of inhibitors, none of them could inhibit EGFRDM effectively. Read More

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December 2018

Site-specific 2D IR spectroscopy: a general approach for the characterization of protein dynamics with high spatial and temporal resolution.

Phys Chem Chem Phys 2018 Dec 14. Epub 2018 Dec 14.

Department of Chemistry, Indiana University, Bloomington, IN 47405, USA. and Indiana University School of Medicine, Indianapolis, IN 46202, USA.

The conformational heterogeneity and dynamics of protein side chains contribute to function, but investigating exactly how is hindered by experimental challenges arising from the fast timescales involved and the spatial heterogeneity of protein structures. The potential of two-dimensional infrared (2D IR) spectroscopy for measuring conformational heterogeneity and dynamics with unprecedented spatial and temporal resolution has motivated extensive effort to develop amino acids with functional groups that have frequency-resolved absorptions to serve as probes of their protein microenvironments. We demonstrate the full advantage of the approach by selective incorporation of the probe p-cyanophenylalanine at six distinct sites in a Src homology 3 domain and the application of 2D IR spectroscopy to site-specifically characterize heterogeneity and dynamics and their contribution to cognate ligand binding. Read More

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December 2018

Plasmonic parametric absorbers.

Opt Lett 2018 Dec;43(24):6013-6016

Exploiting the dynamics of plasmonic parametric resonance (PPR), we introduce the theory of plasmonic parametric absorbers (PPAs). The key insight informing the PPA idea is that in the PPR process a pump field experiences an extinction rate that strongly depends on the intensity of the pump itself, creating two distinct regimes: one of weak absorption under low intensity illumination, and one of strong absorption when the threshold of parametric resonance is met or exceeded. Due to this reverse saturable absorption behavior, PPAs are promising candidates for optical-limiting applications. Read More

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December 2018

Intra-epidemic genome variation in highly pathogenic African swine fever virus (ASFV) from the country of Georgia.

Virol J 2018 Dec 14;15(1):190. Epub 2018 Dec 14.

Laboratory of the Ministry of Agriculture, Tbilisi, Georgia.

Background: African swine fever virus (ASFV) causes an acute hemorrhagic infection in suids with a mortality rate of up to 100%. No vaccine is available and the potential for catastrophic disease in Europe remains elevated due to the ongoing ASF epidemic in Russia and Baltic countries. To date, intra-epidemic whole-genome variation for ASFV has not been reported. Read More

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December 2018

Exploring mental health practice among Traditional health practitioners: a qualitative study in rural Kenya.

BMC Complement Altern Med 2018 Dec 14;18(1):334. Epub 2018 Dec 14.

Africa Mental Health Foundation and Department of Psychiatry, University of Nairobi, Mawensi Road, Off Elgon road, Mawensi Garden, P.O. BOX 48423-00100, Nairobi, Kenya.

Background: Involvement of traditional health practitioners (THPs) in the form of collaboration with the formal health care system is suggested to improve the pathways to mental health care in Kenya, yet understanding of the current traditional practice and THPs' perspectives is lacking. The aim of this study was to explore the views of THPs with respect to their mental health practice.

Methods: This study qualitatively explored the views of THPs, using four focus group discussions (FDGs) each consisting of 8-10 traditional and faith healers, resulting in a total of 36 participants. Read More

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December 2018

Study protocol: individualized music for people with dementia - improvement of quality of life and social participation for people with dementia in institutional care.

BMC Geriatr 2018 Dec 14;18(1):313. Epub 2018 Dec 14.

Department of Counseling and Clinical Intervention, Institute of Psychology, Friedrich Schiller University of Jena, Humboldtstrasse 11, 07743, Jena, Germany.

Background: People with dementia (PwD) experience a range of negative behavioral and psychological symptoms which can lower their quality of life. Because of the increasing prevalence of dementia, interventions that maintain and enhance the quality of life for PwD are needed. Listening to individualized music constitutes a promising non-pharmacological intervention for PwD. Read More

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December 2018

Controlled Fragmentation of Single Molecules with Atomic Force Microscopy by Employing Doubly Charged States.

Phys Rev Lett 2018 Nov;121(22):226101

IBM Research-Zurich, Säumerstrasse 4, 8803 Rüschlikon, Switzerland.

By atom manipulation we performed on-surface chemical reactions of a single molecule on a multilayer insulating film using noncontact atomic force microscopy. The single-electron sensitivity of atomic force microscopy allows us to follow the addition of single electrons to the molecule and the investigation of the reaction products. By performing a novel strategy based on long-lived doubly charged states a single molecule is fragmented. Read More

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November 2018

Venture into Water's No Man's Land: Structural Transformations of Solid H_{2}O under Rapid Compression and Decompression.

Phys Rev Lett 2018 Nov;121(22):225703

Center for High Pressure Science and Technology Advanced Research, Shanghai 201203, China.

Pressure-induced formation of amorphous ices and the low-density amorphous (LDA) to high-density amorphous (HDA) transition have been believed to occur kinetically below a crossover temperature (T_{c}) above which thermodynamically driven crystalline-crystalline (e.g., ice I_{h}-to-II) transitions and crystallization of HDA and LDA are dominant. Read More

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November 2018

Insights into the mechanism of paraoxonase-1: Comparing the reactivity of the six-bladed β-propeller hydrolases.

Biochemistry 2018 Dec 14. Epub 2018 Dec 14.

The mammalian protein paraoxonase-1 (PON1) has been explored as a promising bioscavenger treatment for organophosphorus (OP) agent poisoning, but it is not active enough to protect against many agents. Engineering is limited because PON1's catalytic mechanism is poorly understood; moreover, its native activity and substrate are unknown. PON1 is a calcium-bound six-bladed β-propeller hydrolase that shares high structural homology, a conserved metal-coordinating active site, and substrate specificity overlap with other members of a superfamily that includes squid diisopropylfluorophosphatase (DFPase), bacterial drug responsive protein 35 (Drp35), and mammalian senescence marker protein 30 (SMP30). Read More

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December 2018

Kinetics of Ligand-Protein Dissociation from All-Atom Simulations: Are We There Yet?

Biochemistry 2018 Dec 14. Epub 2018 Dec 14.

Large parallel gains in the development of both computational resources as well as sampling methods have now made it possible to simulate dissociation events in ligand-protein complexes with all--atom resolution. Such encouraging progress, together with the inherent spatiotemporal resolution associated with molecular simulations, has left their use for investigating dissociation processes brimming with potential, both in rational drug design, where it can be an invaluable tool for determining the mechanistic driving forces behind dissociation rate constants, as well as in force-field development, where it can provide a catalog of transient molecular structures on which to refine force-fields. Although much progress has been made in making force-fields more accurate, reducing their error for transient structures along a transition path could yet prove to be a critical development helping to make kinetic predictions much more accurate. Read More

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December 2018

Kounis syndrome and hypersensitivity myocarditis - One and the same? Insights from cardiac magnetic resonance imaging.

J Cardiol Cases 2015 Oct 17;12(4):119-122. Epub 2015 Jul 17.

Royal Adelaide Hospital, Adelaide, Australia.

Myocarditis and acute coronary syndrome are both described in the setting of concurrent hypersensitivity reactions to a variety of allergenic triggers (hypersensitivity myocarditis and Kounis syndrome respectively). Mast cell degranulation is thought to be pivotal in the pathogenesis of both clinical entities. Cardiac magnetic resonance imaging (CMR) has assumed a key role in the assessment of chest pain syndromes, providing a useful non-invasive tool to aid clinical decision-making. Read More

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October 2015

Texture Evolution during Isothermal, Isostrain and Isobaric Loading of Polycrystalline Shape Memory NiTi.

Appl Phys Lett 2017 Jun 23;110(25). Epub 2017 Jun 23.

Advanced Materials Processing and Analysis Center (AMPAC); Materials Science and Engineering Department; Mechanical and Aerospace Engineering Department; University of Central Florida, 12760 Pegasus Drive, Orlando, FL 32816, USA.

neutron diffraction was used to provide insight into martensite variant microstructures during isothermal, isobaric, and isostrain loading in shape memory NiTi. Results show variant microstructures were equivalent for the corresponding strain and more importantly, the reversibility and equivalency was immediately evident in variant microstructures that were first formed isobarically but then reoriented to a near random self-accommodated microstructure following isothermal deformation. Variant microstructures formed isothermally were not significantly affected by a subsequent thermal cycle under constant strain. Read More

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VDAC1 at the crossroads of cell metabolism, apoptosis and cell stress.

Cell Stress 2017 Oct 1;1(1):11-36. Epub 2017 Oct 1.

Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva, 84105, Israel.

This review presents current knowledge related to VDAC1 as a multi-functional mitochondrial protein acting on both sides of the coin, regulating cell life and death, and highlighting these functions in relation to disease. It is now recognized that VDAC1 does not only play a crucial role in regulating the metabolic and energetic functions of mitochondria. The location of VDAC1 at the outer mitochondrial membrane (OMM) allows the control of metabolic cross-talk between mitochondria and the rest of the cell and also enables its interaction with proteins involved in metabolic and survival pathways. Read More

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October 2017

Scalable Inference of Ordinary Differential Equation Models of Biochemical Processes.

Methods Mol Biol 2019 ;1883:385-422

Institute of Computational Biology, Helmholtz Zentrum München, Neuherberg, Germany.

Ordinary differential equation models have become a standard tool for the mechanistic description of biochemical processes. If parameters are inferred from experimental data, such mechanistic models can provide accurate predictions about the behavior of latent variables or the process under new experimental conditions. Complementarily, inference of model structure can be used to identify the most plausible model structure from a set of candidates, and, thus, gain novel biological insight. Read More

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January 2019

Renal toxicity of heavy metals (cadmium and mercury) and their amelioration with ascorbic acid in rabbits.

Environ Sci Pollut Res Int 2018 Dec 13. Epub 2018 Dec 13.

Department of Veterinary Biomedical Sciences, PMAS Arid Agriculture University, Rawalpindi, PK-46300, Pakistan.

Cadmium and mercury are among the most toxic and dangerous environmental pollutants that may cause fatal implications. Vitamin C is an important chain-breaking antioxidant and enzyme co-factor against heavy metals. The objective of the present study was to evaluate the toxicological effects of cadmium chloride, mercuric chloride, and their co-administration on biochemical parameters of blood serum and metal bioaccumulation in kidneys and also to elucidate the protective effect of vitamin C in rabbits against these metals. Read More

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December 2018