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Public Health Action 2018 Dec;8(4):211-217

Boston Children's Hospital, Boston, Massachusetts, USA.

Rural Rwandan hospitals, where thermoregulation is critical yet a challenge for pre-term, low-birth-weight (LBW) or sick newborns. To assess the safety, effectiveness, and feasibility of an inexpensive, reusable, non-electric warmer to complement kangaroo mother care (KMC). Prospective single-arm, non-randomized intervention study. Read More

Am J Cardiol 2019 Feb 8. Epub 2019 Feb 8.

Section of Cardiology, Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, Louisiana. Electronic address:

A man with a history of drug abuse was found down at home and was asystolic. Following restoration of sinus rhythm, a hypothermia protocol brought his temperature to 32.5°C (90. Read More

Perfusion 2019 Feb 17:267659119830014. Epub 2019 Feb 17.

3 Department of Anaesthesiology and Intensive Medicine, Aalborg University Hospital, Aalborg, Denmark.

Introduction:: In massive pulmonary embolism (PE) with circulatory collapse or with cardiac arrest, treatment can be difficult. Often, the diagnosis is unclear, and the time to treatment is crucial. Our institution has had an out-of-hospital team intended for the treatment of accidental hypothermia with extra corporeal membrane oxygenation (ECMO) since 2004. Read More

Resuscitation 2019 Feb 13. Epub 2019 Feb 13.

Emergency Department, Lausanne University Hospital, Lausanne, Switzerland. Electronic address:

Aims: Cardiac arrest related to accidental hypothermia may occur at temperatures below 32 °C. Our goal was to describe the clinical characteristics and outcomes of patients who suffered from witnessed hypothermic cardiac arrest (CA) and assess the occurrence of hypothermic CA as a function of patient body temperature.

Methods: We conducted a systematic review of the literature on cases of hypothermic CA due to rescue collapse. Read More

Resuscitation 2019 Feb 13. Epub 2019 Feb 13.

Center for Resuscitation Science and Department of Emergency Medicine, University of Pennsylvania, Philadelphia, PA, USA. Electronic address:

Aim Of The Study: Regionalization of care for out-of-hospital cardiac arrests (OHCA) may improve patient outcomes. We evaluated inter-hospital variations in post-arrest care provision and the relation between hospital case volume and survival in Pennsylvania.

Methods: This retrospective study (2013-2017) used data from adult OHCA cases in Pennsylvania from the Cardiac Arrest Registry to Enhance Survival. Read More

Mol Metab 2019 Jan 26. Epub 2019 Jan 26.

Faculty of Pharmacy, Université Laval, Quebec City, Qc, Canada; Neurosciences Axis, CHU de Québec-Université Laval Research Center, Quebec City, Qc, Canada. Electronic address:

Objective: Old age is associated with a rise in the incidence of Alzheimer's disease (AD) but also with thermoregulatory deficits. Indicative of a link between the two, hypothermia induces tau hyperphosphorylation. The 3xTg-AD mouse model not only develops tau and amyloid pathologies in the brain but also metabolic and thermoregulatory deficits. Read More

Resuscitation 2019 Feb 12. Epub 2019 Feb 12.

Division of Cardiovascular Medicine, Department of Medicine, State University of New York at Stony Brook, Stony Brook, NY, USA.

Objective We sought to identify the impact of echocardiographic right ventricular (RV) systolic dysfunction on mortality in adults with cardiac arrest (CA). Methods The study population included 147 adults hospitalized with CA who underwent both echocardiogram and coronary angiogram at an academic tertiary medical center. The primary outcome of interest was all-cause in-hospital mortality. Read More

J Biotechnol 2019 Feb 12. Epub 2019 Feb 12.

School of Chemical and Bioprocess Engineering, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland. Electronic address:

Growth-arrested strategies (e.g. hypothermia and hyperosmolarity) have been widely employed to enhance cell-specific productivity (qP) in mammalian cell culture bioprocess. Read More

Am J Transplant 2018 Jul 31. Epub 2018 Jul 31.

Department of Immunology, University of Heidelberg, Germany.

Therapeutic hypothermia, hypothermic pulsatile machine perfusion (MP), and renal-dose dopamine administered to stable brain-dead donors have shown efficacy to reduce the dialysis requirement after kidney transplantation. In a head-to-head comparison of the three major randomized controlled trials in this field, we estimated the number-needed-to-treat for each method, evaluated costs and inquired into special features regarding long-term outcomes. The MP and hypothermia trials used any dialysis requirement during the first post-operative week, while the dopamine trial assessed >1 dialysis session as primary endpoint. Read More

Case Rep Cardiol 2019 15;2019:5845839. Epub 2019 Jan 15.

Department of Medicine, Division of Advanced Heart Failure and Transplant Cardiology, Hospital of the University of Pennsylvania, Philadelphia, PA, USA.

Osborn waves, or J waves, initially described by John Osborn in 1953 in hypothermic dog experiments, are highly sensitive and specific for hypothermia. Initially thought to be secondary to a hypothermia-induced "injury current," they have more recently been attributed to a voltage differential between epicardial and endocardial potassium (I) currents. While the exact conditions required to induce such waves have been debated, numerous clinical scenarios of environmental and iatrogenic hypothermia have been described. Read More

J Orthop Surg Res 2019 Feb 14;14(1):49. Epub 2019 Feb 14.

Department of Orthopedics, Spaarne Gasthuis, Spaarnepoort 1, 2134 TM, Hoofddorp, The Netherlands.

Background: The aim of this study was to define deep tissue temperature during cryotherapy in postoperative hip fracture patients, by using measured skin temperature as input parameter for a simple numerical model. Second, an association was investigated between pain and tissue temperature distribution, to assess cryotherapy-induced analgesia of soft tissue-derived pain.

Methods: Data from 35 participants in an ongoing trial was used. Read More

Anaesth Crit Care Pain Med 2019 Feb 11. Epub 2019 Feb 11.

Department of anaesthesia and intensive care medicine, CHRU La Cavale Blanche, Brest, France; UFR de médecine, Université de Bretagne occidentale, Brest, France; ANZIC research centre, Monash University, Melbourne, Victoria, Australia. Electronic address:

PLoS One 2019 14;14(2):e0211910. Epub 2019 Feb 14.

Department of Clinical Pharmacy, University Medical Center Utrecht, Utrecht, the Netherlands.

Objective: Morphine is a commonly used drug in encephalopathic neonates treated with therapeutic hypothermia after perinatal asphyxia. Pharmacokinetics and optimal dosing of morphine in this population are largely unknown. The objective of this study was to describe pharmacokinetics of morphine and its metabolites morphine-3-glucuronide and morphine-6-glucuronide in encephalopathic neonates treated with therapeutic hypothermia and to develop pharmacokinetics based dosing guidelines for this population. Read More

Stem Cells Dev 2019 Feb 14. Epub 2019 Feb 14.

Mossakowski Medical Research Centre, Polish Academy of Sciences, NeuroRepair Department, Warsaw, Mazowsze, Poland.

White matter damage persists in hypoxic-ischemic newborns even when treated with hypothermia. We have previously shown that intra-ventricular delivery of human glial progenitors (GPs) at the neonatal stage is capable of replacing abnormal host glia and rescuing the lifespan of dysmyelinated mice. However, such transplantation in the human brain poses significant challenges as related to high-volume ventricles and long cell migration distances. Read More

J Palliat Med 2019 Feb 13. Epub 2019 Feb 13.

4 Palliative Medicine, Stanford University School of Medicine, Stanford, California.

Background: Night sweats significantly impact the quality of life for cancer patients and are often resistant to treatment. Cannabinoids have been shown to modulate cytokine activity and produce hypothermia in animal models, suggesting that they may be a promising candidate for palliation of night sweats in patients with oncologic disease.

Objective: Assess efficacy of the oral cannabinoid, dronabinol, for palliation of night sweats in cancer patients. Read More

Am J Physiol Regul Integr Comp Physiol 2019 Feb 13. Epub 2019 Feb 13.

University of Tsukuba, Faculty of Health and Sport Sciences, Japan.

Hypothermia can occur during aquatic exercise, despite production of significant amounts of heat by the active muscles. Because the characteristics of human thermoregulatory responses to cold during exercise have not been fully elucidated, we investigated the effect of low-intensity exercise on the shivering response to core cooling in cool water. Eight healthy young men (24±3 years) were cooled through cool water immersion while resting (rest trial) and during loadless pedaling on a water cycle ergometer (exercise trial). Read More

Ther Hypothermia Temp Manag 2019 Feb 13. Epub 2019 Feb 13.

Med Biol Eng Comput 2019 Feb 12. Epub 2019 Feb 12.

National Center for International Research on Green Optoelectronics, South China Normal University, Waihuan West Road 378, Guangzhou, 510006, People's Republic of China.

Therapeutic hypothermia is a treatment method to reduce brain injuries after stroke, especially for cerebral ischemia. This study investigates in the temperature distribution of the head within selective brain cooling (SBC). Anatomically accurate geometries based on CT images of head and neck regions are used to develop the 3D geometry and physical model for the finite element modeling. Read More

Clin Chem Lab Med 2019 Feb 12. Epub 2019 Feb 12.

Department of Maternal, Fetal and Neonatal Medicine, C. Arrigo Children's Hospital, Alessandria, Italy.

Background Perinatal asphyxia is a major cause of mortality and morbidity in neonates: The aim of the present study was to investigate, by means of longitudinal assessment of urinary S100B, the effectiveness of hypothermia, in infants complicated by perinatal asphyxia and hypoxic-ischemic encephalopathy. Methods We performed a retrospective case-control study in 108 asphyxiated infants, admitted to nine tertiary departments for neonatal intensive care from January 2004 to July 2017, of whom 54 underwent hypothermia treatment and 54 did not. The concentrations of S100B protein in urine were measured using an immunoluminometric assay at first urination and 4, 8, 12, 16, 20, 24, 48, 72, 96, 108 and 120 h after birth. Read More

J Dev Behav Pediatr 2019 Feb/Mar;40(2):92-98

Division of Internal Medicine and Pediatrics, University of California, Los Angeles, CA.

Objective: To examine the medical assessment and triage of pediatric patients with anorexia nervosa (AN) initially seen in primary care.

Methods: A retrospective cohort study was conducted for all pediatric patients with AN who had eating/weight concerns and initially identified symptoms in primary care in a single health care system between January 1, 2010, and December 31, 2016. Information on presenting concern, medical assessment/laboratory tests, clinical diagnoses, treatment recommendations, and referrals were abstracted from the medical record. Read More

Clin Exp Emerg Med 2019 Feb 12. Epub 2019 Feb 12.

Department of Emergency Medicine, University of Pittsburgh, Pittsburgh, PA, USA.

A 34-year-old male was brought to the hospital with a chest gunshot wound. Pulseless upon arrival, blood pressure was absent for 10 minutes. A thoracotomy resulted in return of spontaneous circulation. Read More

Semin Neurol 2019 Feb 11;39(1):137-148. Epub 2019 Feb 11.

Division of Emergency Medicine, Department of Surgery, University of Vermont College of Medicine, Burlington, Vermont.

The study of wilderness medicine is within the scope of medical care in the austere environment and addresses medicine as practiced in the setting of delayed access to definitive medical care, hostile environment, limited equipment, and inherent risks to the patient and/or rescuers. Part of this topic includes the care of patients with neurologic illness and/or injury.We will address the five most important skills of a wilderness medicine professional: decision making, prevention, preparation, protocol development, and education by applying the principles to select common neurologic problems that occur in the extended environment: traumatic brain injury, dehydration, hyponatremia, heat illness, hypothermia, spine injury, and lightning injury. Read More

Pediatr Res 2019 Jan 30. Epub 2019 Jan 30.

The Department of Physiology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand.

J Neonatal Perinatal Med 2019 Feb 4. Epub 2019 Feb 4.

Division of Neonatal-Perinatal Medicine, Wayne State University, Children's Hospital of Michigan and Hutzel Women's Hospital, Detroit, MI, USA.

Background: Perinatal asphyxia is a prominent cause of neonatal mortality in the developing world. Growth in head circumference is associated with improved neurodevelopment. Previous studies found a positive correlation between additional dietary supplementation and growth in head circumference among newborns with perinatal brain injury. Read More

J Small Anim Pract 2019 Feb 10. Epub 2019 Feb 10.

Department of Mathematical Modelling, Statistics and Bioinformatics, Ghent University, 9000 Ghent, Belgium.

Objectives: To describe the health disorders seen in captivity in 206 pet prairie dogs.

Materials And Methods: Review of medical records of pet prairie dogs that were presented to a veterinary clinic in the period January 2002 to December 2011.

Results: The most frequent diseases were respiratory (28. Read More

Thorac Cardiovasc Surg 2019 Feb 9. Epub 2019 Feb 9.

Benha University, Benha, Egypt.

Background:  The timing of surgical repair of tetralogy of Fallot (TOF) is a key to alleviate complications and for long-term survival. Total correction was usually performed at the age of 6 months or older under the notion of decreasing the surgical risk. However, avoiding palliation with an aortopulmonary shunt and early correction of systemic hypoxia appear to be of more benefit than the inborn surgical risk in low body weight patients. Read More

Wiad Lek 2018 ;71(9):1690-1694

Higher State Educational Institution Of Ukraine «Uzhhorod National University», Uzhhorod, Ukraine.

Objective: Introduction: Hyperplasia of the thyroid gland is recetly widespread, especially among women, with pathology. At structural changes of a gland there are various types of violations of the hormonal status of the organism - from hyperthyroidism through euthyroidism to hypothermia. The aim: The article aims at investigation of the peculiarities of the influence of mineral water "Naftusia" on the immunological status of women with non-calcular cholecystitis and hyperplasia of the thyroid gland, as well as the substantiation of the possibilities of using balneotherapy in the treatment of this pathology on the basis of the obtained data. Read More

Wilderness Environ Med 2019 Feb 5. Epub 2019 Feb 5.

Laboratory for Exercise and Environmental Medicine, Faculty of Kinesiology and Recreation Management, University of Manitoba, Winnipeg, Canada; Department of Physical Therapy, College of Rehabilitation Sciences, University of Manitoba, Winnipeg, Canada. Electronic address:

Introduction: To evaluate the rewarming effectiveness of a similar amount of heat (from a charcoal heater) applied to either the head or torso in a human model for severe hypothermia in which shivering is pharmacologically inhibited in mildly hypothermic subjects.

Methods: Six male subjects were cooled on 3 different occasions, each in 8°C water for 60 min, or to a lowest core temperature of 35°C. Shivering was inhibited by intravenous meperidine (1. Read More

J Pineal Res 2019 Feb 8:e12565. Epub 2019 Feb 8.

Department of Molecular and Developmental Medicine, University of Siena.

Introduction: Neonates with hypoxic-ischemic encephalopathy (HIE) undergoing hypothermia may benefit from adjunctive therapy with melatonin. However, melatonin safety, pharmacokinetics (PK), and dosage in this sensitive population is still unknown.

Methods And Results: This study assessed the PK and safety of melatonin enteral administration to neonates with HIE undergoing hypothermia. Read More

J Exp Neurosci 2019 21;13:1179069518824852. Epub 2019 Jan 21.

Jefferson Underclassman Multidisciplinary Laboratory (JUMP Lab), Thomas Jefferson High School for Science and Technology, Alexandria, VA, USA.

Background/main Objectives: No effective strategy exists to treat the well-recognized, devastating impact of traumatic brain injury (TBI) and chronic traumatic encephalopathy (CTE), which is the brain degeneration likely caused by repeated head trauma. The goals of this project were (1) to study the effects of single and recurrent TBI (rTBI) on (a) life span, (b) response to sedatives, and (c) behavioral responses to light and gravity and (2) to determine whether therapeutic hypothermia can mitigate the deleterious effects of TBI.

Methods: Five experimental groups were created: (1) control, (2) single TBI or concussion; (3) concussion + hypothermia, (4) rTBI, and (5) rTBI + hypothermia. Read More

Pediatr Res 2019 Jan 16. Epub 2019 Jan 16.

Department of Physiology, School of Medical Sciences, University of Auckland, Auckland, New Zealand.

Infants with moderate to severe neonatal encephalopathy (NE) benefit significantly from therapeutic hypothermia, with reduced risk of death or disability. However, the need for therapeutic hypothermia for infants with milder NE remains unclear. It has been suggested that these infants should not be offered therapeutic hypothermia as they may not be at risk for adverse neurodevelopmental outcome and that the balance of risk against potential benefit is unknown. Read More

Medicine (Baltimore) 2019 Feb;98(6):e14392

Department of Anesthesiology.

Background: A majority of reports in the past decade have demonstrated that perioperative hypothermia increases susceptibility to surgical site infection (SSI). However, in recent years, an increasing number of studies did not find an association between hypothermia and the risk of SSI. These contradictory results have given rise to a conflicting issue on whether perioperative hypothermia is associated with SSI risk in surgical patients. Read More

Anaesth Crit Care Pain Med 2019 Feb 5. Epub 2019 Feb 5.

Grenoble Alps trauma centre, department of anaesthesiology and intensive care medicine, Grenoble university hospital, 38000, Grenoble, France.

Ther Hypothermia Temp Manag 2019 Feb 6. Epub 2019 Feb 6.

1 Mission Neuroscience Institute (NSI), Mission Hospital, Mission Viejo, California.

Physiology (Bethesda) 2019 Mar;34(2):101-111

Department of Cell and Developmental Biology, University of Colorado School of Medicine , Aurora, Colorado.

Suspended animation for deep-space travelers is moving out of the realm of science fiction. Two approaches are considered: the first elaborates the current medical practice of therapeutic hypothermia; the second invokes the cascade of metabolic processes naturally employed by hibernators. We explore the basis and evidence behind each approach and argue that mimicry of natural hibernation will be critical to overcome the innate limitations of human physiology for long-duration space travel. Read More

Acta Paediatr 2019 Feb 5. Epub 2019 Feb 5.

Maine Medical Center, Portland, Maine.

Aim: Concerns for infant destabilization often prohibit parental holding of infants during therapeutic hypothermia (TH). We assessed the feasibility of maternal holding during TH, as the inability to hold can impede bonding.

Methods: Vital signs were assessed in stable infants before, at two-minute intervals during and 30 minutes after a single 30-minute holding session. Read More

Pediatr Crit Care Med 2019 Feb;20(2):166-171

Department of Pediatrics, University of California at Davis, Sacramento, CA.

Objectives: Therapeutic hypothermia is standard of care in management of moderate/severe hypoxic-ischemic encephalopathy. Persistent pulmonary hypertension of the newborn is associated with hypoxic-ischemic encephalopathy and is exacerbated by hypoxemia and hypercarbia. Gas exchange is assessed by arterial blood gas analysis (with/without correction for body temperature), pulse oximetry, and end-tidal CO2. Read More

Curr Opin Pediatr 2019 Jan 31. Epub 2019 Jan 31.

Department Pediatrics, UCSF Benioff Children's Hospital.

Purpose Of Review: With the advent of therapeutic hypothermia for treatment of hypoxic ischemic encephalopathy, and improvements in neuroimaging and bedside neuromonitoring, a new era of neonatal brain-focused care has emerged in recent years. We describe the development of the first neurointensive care nursery (NICN) as a model for comanagement of neonates with identified neurologic risk factors by a multidisciplinary team constituted of neurologists, neonatologists, specialized nurses, and others with the goal of optimizing management, preventing secondary injury and maximizing long-term outcomes.

Recent Findings: Optimizing brain metabolic environment and perfusion and preventing secondary brain injury are key to neurocritical care. Read More

Neuropharmacology 2019 Feb 1;149:55-65. Epub 2019 Feb 1.

Center of Neuroscience, Surgery and Trauma, Blizard Institute, Barts and London School of Medicine and Dentistry, Queen Mary University of London, UK. Electronic address:

Neonates can develop hypoxic-ischaemic encephalopathy (HIE) due to lack of blood supply or oxygen, resulting in a major cause of death and disability among term newborns. However, current definitive treatment of therapeutic hypothermia, will only benefit one out of nine babies. Furthermore, the mechanisms of HIE and therapeutic hypothermia are not fully understood. Read More

J Matern Fetal Neonatal Med 2019 Feb 3:1-221. Epub 2019 Feb 3.

a Division of Newborn Medicine , Department of Pediatrics , McGill University , Montreal , Canada.

Aim: To study the consequences of restricting fluid and sodium intake in asphyxiated newborns treated with hypothermia.

Methods: We conducted a retrospective cohort study of asphyxiated newborns treated with hypothermia from 2009 to 2015. The fluid, parenteral nutrition and sodium as well as the urine produced per day were calculated. Read More

Paediatr Anaesth 2019 Feb 3. Epub 2019 Feb 3.

Department of Anaesthesia, Congenital Cardiac Unit, Marie Lannelongue Hospital, 133 Avenue de la Resistance, 92350, Le Plessis-Robinson.

A primary goal of improving neonatal cardiopulmonary bypass has been making the circuit smaller and reduce the blood contacting surfaces. As bypass circuit size has decreased, bloodless surgery has become possible even in neonates. Since transfusion guidelines are difficult to construct based on existing literature, these technical advances should be taken advantage of in conjunction with an individualized transfusion scheme, based on monitoring of oxygen availability to the tissues. Read More

Med Gas Res 2018 Oct-Dec;8(4):172-175. Epub 2019 Jan 9.

Department of Neurosurgery & Brain and Nerve Research Laboratory, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China.

Cerebrovascular diseases have a heavy burden on society and the family. At present, in the treatment of cerebrovascular diseases, the recognized effective treatment method is a thrombolytic therapy after cerebral infarction, but limited to the time window problem, many patients cannot benefit. Other treatments for cerebrovascular disease are still in the exploration stage. Read More

R I Med J (2013) 2019 Feb 1;102(1):28-32. Epub 2019 Feb 1.

Associate Professor, Department of Emergency Medicine, Alpert Medical School of Brown University.

[Full article available at http://rimed.org/rimedicaljournal-2019-02.asp]. Read More

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2019 Jan;31(1):55-60

Department of Emergency, Second Affiliated Hospital of Soochow University, Suzhou 215008, Jiangsu, China. Corresponding author: Liu Lijun, Email:

Objective: To investigate the effect of III-type phosphatidylinositide 3 kinase (PI3K) pathway adjusting autophagy on brain damage mechanism after cardiopulmonary resuscitation (CPR) and hypothermia treatment.

Methods: The asphyxia induce cardiac arrest-CPR model was reproduced on healthy male Sprague-Dawley (SD) rats. Sixty rats after restoration of spontaneous circulation (ROSC) were randomly divided into normothermia group, therapeutic hypothermia group and PI3K inhibitor 3-methyl adenine (3-MA) pretreatment group, differentiated by 24 hours and 48 hours after ROSC. Read More

Zhonghua Nei Ke Za Zhi 2019 Feb;58(2):108-118

Department of Critical Care Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.

To establish the experts consensus on the management of delirium in critically ill patients. A special committee was set up by 15 experts from the Chinese Critical Hypothermia-Sedation Therapy Study Group. Each statement was assessed based on the GRADE (Grading of Recommendations Assessment, Development, and Evaluation) principle. Read More

Hamostaseologie 2019 Feb 31;39(1):20-27. Epub 2019 Jan 31.

Medical ICU, University Hospital, Leipzig, Germany.

Trauma-induced coagulopathy (TIC) is a heterogeneous entity that contributes to a significant morbidity and mortality following trauma. The activated protein C system, endotheliopathy and platelet dysfunction have been implicated in the pathogenesis of TIC, although there are still controversies on the exact pathogenesis. TIC can be modified by hypoperfusion, acidosis, hypothermia, haemodilution, underlying disease conditions, pre-injury medications as well as genetic predispositions. Read More

Eur J Med Chem 2019 Jan 22;166:144-158. Epub 2019 Jan 22.

Department of Drug Technology and Pharmaceutical Biotechnology, Faculty of Pharmacy, Medical University of Warsaw, 1, Banacha Street, 02-097 Warsaw, Poland.

The study enabled obtaining a number of new derivatives of 4-aryl-pyrido[1,2-c]pyrimidine 9.1-9.27 having conformationally restricted tryptamine moiety. Read More

J Biotechnol 2019 Jan 28;294:1-13. Epub 2019 Jan 28.

Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield S1 3JD, UK. Electronic address:

Synthetic promoters are an attractive alternative for use in mammalian hosts such as CHO cells as they can be designed de novo with user-defined functionalities. In this study, we describe and validate a method for bioprocess-directed design of synthetic promoters utilizing CHO genomic sequence information. We designed promoters with two objective features, (i) constitutive high-level recombinant gene transcription, and (ii) upregulated transcription under mild hypothermia or late-stage culture. Read More

Rev Lat Am Enfermagem 2019 Jan 17;27:e3103. Epub 2019 Jan 17.

Hospital Sarah Kubitschek, Brasília, DF, Brazil.

Objective: to compare the efficacy of three active heating methods in the prevention of intraoperative hypothermia in open gastroenterological surgeries.

Method: randomized clinical trial with a sample of 75 patients, whose initial body temperature measured by a tympanic thermometer. Esophageal temperature <36ºC was considered hypothermic. Read More

J Clin Neurophysiol 2019 Jan 28. Epub 2019 Jan 28.

Departments of Clinical Neurophysiology and.

Purpose: Monitoring of somatosensory evoked potentials (SSEPs) serves as an early warning system to detect spinal cord injury and is correlated with postoperative sensory findings. It is an indirect indicator of motor function. This study aimed to evaluate the usefulness of intraoperative SSEPs monitoring as a stand-alone tool during spinal surgeries when motor evoked potentials are not available, to prevent and predict new postoperative neurologic deficits. Read More