J Clin Lab Anal 2022 Jun 11:e24514. Epub 2022 Jun 11.
Department of Otolaryngology Head and Neck Surgery, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
Background: Having emerged as the most abundant posttranscriptional internal mRNA modification in eukaryotes, N6-methyladenosine (m A) has attracted tremendous scientific interest in recent years. However, the functional importance of the m A methylation machinery in ferroptosis regulation in hypopharyngeal squamous cell carcinoma (HPSCC) remains unclear.
Methods: We herein performed bioinformatic analysis, cell biological analyses, transcriptome-wide m A sequencing (m A-seq, MeRIP-seq), RNA sequencing (RNA-seq), and RNA immunoprecipitation sequencing (RIP-seq), followed by m A dot blot, MeRIP-qPCR, RIP-qPCR, and dual-luciferase reporter assays. Read More