18,092,229 results match your criteria Human reproduction Oxford England[Journal]

  • Page 1 of 361845

Twenty-five years of research about adipogenic adenoviruses: A systematic review.

Obes Rev 2018 Dec 18. Epub 2018 Dec 18.

Department of Nutritional Sciences, Texas Tech University, Lubbock, TX, USA.

Infectious etiology is implicated in chronic diseases such as gastric ulcer or atherosclerosis. However, "infection" is a recent term in the field of obesity. Since the first report in 1982 of obesity due to infection, several microbes have been linked to obesity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/obr.12808DOI Listing
December 2018

Intra-articular Corticosteroids for Knee Pain-What Have We Learned from the Equine Athlete and Current Best Practice.

J Knee Surg 2018 Dec 18. Epub 2018 Dec 18.

Department of Orthopaedic Surgery, Division of Sports Medicine, Brigham and Women's Hospital, Boston, Massachusetts.

The use of intra-articular corticosteroids for traumatic arthritis and osteoarthritis (OA) is common in the horse. The beneficial and deleterious effects of the principal corticosteroids used betamethasone esters (Celestone [Soluspan], methylprednisolone acetate [Depo Medrol], and triamcinolone acetonide [TA] [Vetalog or Kenalog]) have been defined for the horse. While TA has both disease-modifying as well as symptom-modifying effects, methyl prednisolone acetate has deleterious effects on the articular cartilage. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1055/s-0038-1676449DOI Listing
December 2018

Osteochondral Allograft Transplantation: Identifying the Biomechanical Impact of Using Shorter Grafts and Pulsatile Lavage on Graft Stability.

J Knee Surg 2018 Dec 18. Epub 2018 Dec 18.

Division of Sports Medicine, Department of Orthopaedic Surgery, Warren Alpert Medical School, Brown University, Providence, Rhode Island.

This study seeks to identify the ability of shorter osteochondral allografts (OCAs) to resist displacement/failure. Additionally, this study seeks to evaluate the effect of pulsatile lavage (PL) on the biomechanical stability of the OCA. Fifteen-millimeter diameter, human cadaveric, OCAs of 4, 7, and 10 mm in depth were harvested for comparison of resistance to compressive and tensile loads. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1055/s-0038-1676501DOI Listing
December 2018

Inhibitory Effect of Damulin B from Gynostemma pentaphyllum on Human Lung Cancer Cells.

Planta Med 2018 Dec 18. Epub 2018 Dec 18.

School of Pharmacy, Minzu University of China, Beijing, China.

Damulin B, a dammarane-type saponin from steamed , exhibits the strongest activity against human lung carcinoma A549 cells among the isolated active saponins. In this study, the structure-activity relationship of a series of saponin compounds was discussed. The inhibitory effect of damulin B on human lung cancer A549 and H1299 cells was investigated from apoptosis, cell cycle, and migration aspects. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1055/a-0810-7738DOI Listing
December 2018

A Bufadienolide-Enriched Fraction of Bryophyllum pinnatum Inhibits Human Myometrial Contractility In Vitro.

Planta Med 2018 Dec 18. Epub 2018 Dec 18.

Department of Obstetrics, University Hospital Zurich, Zurich, Switzerland.

has been used since the 1970s to prevent premature labour, first in anthroposophic hospitals and, more recently, also in the main Swiss perinatal centres. However, it is not known which compounds in leaves contribute to the tocolytic effect. Here we studied the effects of a flavonoid-enriched fraction, the corresponding flavonoid aglycon mixture, a bufadienolide-enriched fraction, and leaf press juice on human myometrial contractility . Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1055/a-0810-7704DOI Listing
December 2018

[50 Years ago: First Successful Treatment of Acute Allograft Rejection after a Human Heart Transplantation].

Authors:
Walter G Land

Dtsch Med Wochenschr 2018 Dec 18;143(25):1864-1865. Epub 2018 Dec 18.

The first clinical use of the "Munich antilymphocyte globulin" (ALG) at the occasion of the first successful human heart transplantation is briefly described. The cardiac transplantation was carried out by Christiaan Barnard and his team in Cape Town, South Africa, in 1968. The patient developed an acute allograft rejection which could be successfully reversed within three weeks using the intravenous administration of ALG. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1055/a-0573-5623DOI Listing
December 2018

[It can not be healthy not to be ill].

Dtsch Med Wochenschr 2018 Dec 18;143(25):1826-1831. Epub 2018 Dec 18.

This article is written in remembrance of Karl Valentin's 70th anniversary of his death. He was a comedian and song writer suffering from vegetative dystonia and depression lifelong. This hypochondriac developed extreme self observation and had anxiety to get ill permanently. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1055/a-0649-0274DOI Listing
December 2018

Garcinia Multiflora Inhibits FPR1-Mediated Neutrophil Activation and Protects Against Acute Lung Injury.

Cell Physiol Biochem 2018 Dec 12;51(6):2776-2793. Epub 2018 Dec 12.

Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan,

Background/aims: Formyl peptide receptors (FPRs) recognize different endogenous and exogenous molecular stimuli and mediate neutrophil activation. Dysregulation of excessive neutrophil activation and the resulting immune responses can induce acute lung injury (ALI) in the host. Accordingly, one promising approach to the treatment of neutrophil-dominated inflammatory diseases involves therapeutic FPR1 inhibition. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1159/000495970DOI Listing
December 2018

Long Noncoding RNA HOTTIP Promotes Mouse Hepatic Stellate Cell Activation via Downregulating miR-148a.

Cell Physiol Biochem 2018 Dec 13;51(6):2814-2828. Epub 2018 Dec 13.

Background/aims: HOTTIP is a critical modulator in human diseases including liver cancer, but its role and molecular biological mechanisms in liver fibrosis are still unclear.

Methods: The expression profile of HOTTIP during the progression of liver fibrosis was detected in human liver samples and in CCl4-treated mice using qRT-PCR. The expressing sh-HOTTIP adenoviral vector was used to reduce HOTTIP levels in vivo. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1159/000496012DOI Listing
December 2018

Loss of Tumor Suppressor Gene Function in Human Cancer: An Overview.

Cell Physiol Biochem 2018 Dec 12;51(6):2647-2693. Epub 2018 Dec 12.

Research Institute of Pharmaceutical Science, Department of Pharmacy, Seoul National University College of Pharmacy, Seoul, Republic of

Cancer is a disease caused by the accumulation of genetic and epigenetic changes in two types of genes: tumor suppressor genes (TSGs) and proto-oncogenes. Extensive research has been conducted over the last few decades to elucidate the role of TSGs in cancer development. In cancer, loss of TSG function occurs via the deletion or inactivation of two alleles, according to Knudson's two-hit model hypothesis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1159/000495956DOI Listing
December 2018

FOXD1 Promotes Cell Growth and Metastasis by Activation of Vimentin in NSCLC.

Cell Physiol Biochem 2018 Dec 12;51(6):2716-2731. Epub 2018 Dec 12.

Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.

Background/aims: Forkhead box D1 (FOXD1) has a well-established role in early embryonic development and organogenesis and functions as an oncogene in several cancers. However, the clinical significance and biological roles of FOXD1 in non-small cell lung cancer (NSCLC) remain largely unknown.

Methods: A total of 264 primary NSCLC tissue samples were collected. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1159/000495962DOI Listing
December 2018

MACROD1/LRP16 Enhances LPS-Stimulated Inflammatory Responses by Up-Regulating a Rac1-Dependent Pathway in Adipocytes.

Cell Physiol Biochem 2018 Dec 11;51(6):2591-2603. Epub 2018 Dec 11.

Department of Endocrinology, Chinese PLA General Hospital, Beijing,

Background/aims: Chronic inflammation contributes to the development of type 2 diabetes mellitus by targeting the insulin receptor substrate protein-1 (IRS-1) signaling pathway. Previous studies showed that Leukemia related protein 16 (LRP16) reduced insulin stimulated glucose uptake in adipocytes by impairing the IRS-1 signaling pathway. We explored the mechanism by which LRP16 promotes the inflammatory response. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1159/000495931DOI Listing
December 2018

Characterization and Beige Adipogenic Potential of Human Embryo White Adipose Tissue-Derived Stem Cells.

Cell Physiol Biochem 2018 Dec 14;51(6):2900-2915. Epub 2018 Dec 14.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.

Background/aims: Brown and beige adipocytes are widely recognized as potential therapeutic targets to treat obesity and related metabolic disorders, and the recruitment of brown and beige adipocytes is an essential aspect that requires attention. Although many methods of activating brown adipocytes or generating beige adipocytes have been reported, the limited number and sources are the biggest challenges. The number of white adipocytes is much greater than the number of brown adipocytes, both in human adults and fetuses. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1159/000496042DOI Listing
December 2018

lncRNA MEG3 Suppresses the Tumorigenesis of Hemangioma by Sponging miR-494 and Regulating PTEN/ PI3K/AKT Pathway.

Cell Physiol Biochem 2018 Dec 14;51(6):2872-2886. Epub 2018 Dec 14.

Department of General Surgery, Xinhua Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai,

Background/aims: Dysregulation of long noncoding RNAs (lncRNAs) is associated with the proliferation and metastasis in a variety of cancers, of which lncRNA maternally expressed gene 3 (MEG3) has been indicated as a tumor suppressor in multiple malignancies. However, the underlying mechanisms by which MEG3 contributes to human hemangiomas (HAs) remain undetermined.

Methods: qRT-PCR analysis was performed to examine the expression levels of MEG3 and VEGF in proliferating or involuting phase HAs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1159/000496040DOI Listing
December 2018

Expression of PRDX6 Correlates with Migration and Invasiveness of Colorectal Cancer Cells.

Cell Physiol Biochem 2018 Dec 11;51(6):2616-2630. Epub 2018 Dec 11.

Institute of Nursing and Department of Nursing, Chang Gung Institute of Technology Chiayi Campus, Chiayi, Taiwan.

Background/aims: Colorectal cancer (CRC) is the third most common type of cancer and the second leading cause of cancer-related deaths worldwide. PRDXs are antioxidant enzymes that play an important role in cell differentiation, proliferation and apoptosis and have diverse functions in malignancy development. However, the mechanism of aberrant overexpression of PRDX6 in CRC remains unclear. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1159/000495934DOI Listing
December 2018

Inhibition of Cyclin D1 Expression in Human Glioblastoma Cells is Associated with Increased Temozolomide Chemosensitivity.

Cell Physiol Biochem 2018 Dec 11;51(6):2496-2508. Epub 2018 Dec 11.

Department of Neurosurgery, Changzheng Hospital, Second Military Medical University, Shanghai,

Background/aims: Cyclin D1 (CCND1) is frequently overexpressed in malignant gliomas. We have previously shown ectopic overexpression of CCND1 in human malignant gliomas cell lines.

Methods: Quantitative reverse transcriptase PCR (qRT-PCR) and Western Blot (WB) was performed to investigate the expression of CCND1 in glioma tissues and cell lines. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1159/000495920DOI Listing
December 2018

Sinoporphyrin Sodium-Mediated Sonodynamic Therapy Inhibits RIP3 Expression and Induces Apoptosis in the H446 Small Cell Lung Cancer Cell Line.

Cell Physiol Biochem 2018 Dec 14;51(6):2938-2954. Epub 2018 Dec 14.

Department of Medical Oncology, Harbin Medical University Cancer Hospital, Harbin,

Background/aims: Sonodynamic therapy (SDT) is expected to be a new method to solve the clinical problems caused by advanced metastasis in patients with lung cancer. The use of ultrasound has the advantage of being noninvasive, with deep-penetration properties. This study explored the anti-tumor effect of SDT with a new sonosensitizer, sinoporphyrin sodium (DVDMS), on the human small cell lung cancer H446 cell line in vitro and in vivo. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1159/000496045DOI Listing
December 2018

Effect of Ionic Strength, pH and Chemical Displacers on the Percentage Protein Binding of Protein-Bound Uremic Toxins.

Blood Purif 2018 Dec 18:1-10. Epub 2018 Dec 18.

Background And Objectives: While trying to optimize the dialysis clearances of protein-bound uremic toxins (PBUTs), their percentage protein binding (% PB) is an important parameter. We evaluated the effects of ionic strength, pH change and chemical displacers on the dissociation of PBUTs from albumin in vitro.

Methods: PBUTs, such as 3-Carboxy-4-methyl-5-propyl-2-furan-propanoic acid (CMPF), p-cresylsulfate (PCS), indoxyl sulfate (IS) and indole-3-acetic acid (IAA), were spiked with human serum albumin (HSA) solution prepared with different Nacl concentrations and pH values or in the presence of a series of chemical displacers. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1159/000495343DOI Listing
December 2018

The FOXK1-CCDC43 Axis Promotes the Invasion and Metastasis of Colorectal Cancer Cells.

Cell Physiol Biochem 2018 Dec 11;51(6):2547-2563. Epub 2018 Dec 11.

Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou,

Background/aims: The CCDC43 gene is conserved in human, rhesus monkey, mouse and zebrafish. Bioinformatics studies have demonstrated the abnormal expression of CCDC43 gene in colorectal cancer (CRC). However, the role and molecular mechanism of CCDC43 in CRC remain unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1159/000495924DOI Listing
December 2018

Synthesis and fabrication of a keratin-conjugated insulin hydrogel for the enhancement of wound healing.

Colloids Surf B Biointerfaces 2018 Dec 11;175:436-444. Epub 2018 Dec 11.

Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China. Electronic address:

Accelerating and regulating collagen formation during wound healing repair is key issues for skin regeneration. Insulin can promote the healing of damaged skin by stimulating cellular migration and angiogenesis. Here, human hair keratin-conjugated insulin was synthesized to enhance full-thickness skin regeneration based on the excellent wound healing and hemostatic effects of keratin and the collagen deposition regulation ability of insulin. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.colsurfb.2018.12.020DOI Listing
December 2018

Relationships between the bioavailability of polybrominated diphenyl ethers in soils measured with female C57BL/6 mice and the bioaccessibility determined using five in vitro methods.

Environ Int 2018 Dec 15;123:337-344. Epub 2018 Dec 15.

Guangzhou Key Laboratory of Environmental Catalysis and Pollution Control, School of Environmental Science and Engineering, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou 510006, Guangdong, PR China. Electronic address:

Several in vitro methods for simulating human gastrointestinal digestion have been validated for predicting the bioavailability of heavy metals, but the methods for successfully predicting the bioavailability of organic pollutants are still limited. In this study, we used an adapted fasting in vitro digestion method (Fa-VDM) from the Simulator of the Human Intestinal Microbial Ecosystem and four other in vitro methods comprising In Vitro Gastrointestinal, a physiologically-based extraction test, the unified BARGE method, and Deutsches Institut für Normung e.V. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envint.2018.12.022DOI Listing
December 2018

Overt congruent facial reaction to dynamic emotional expressions in 9-10-month-old infants.

Infant Behav Dev 2018 Dec 15;54:48-56. Epub 2018 Dec 15.

The 21st Century Program, Kyushu University, Fukuoka City, Fukuoka Prefecture, Japan.

The current study aimed to extend the understanding of the early development of spontaneous facial reactions toward observed facial expressions. Forty-six 9- to 10-month-old infants observed video clips of dynamic human facial expressions that were artificially created with morphing technology. The infants' facial responses were recorded, and the movements of the facial action unit 12 (e. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.infbeh.2018.12.002DOI Listing
December 2018

Discovery of new potent hits against intracellular Trypanosoma cruzi by QSAR-based virtual screening.

Eur J Med Chem 2018 Nov 29;163:649-659. Epub 2018 Nov 29.

LabMol - Laboratory for Molecular Modeling and Drug Design, Faculdade de Farmacia, Universidade Federal de Goiás - UFG, Rua 240, Qd.87, Goiania, GO, 74605-510, Brazil. Electronic address:

Chagas disease is a neglected tropical disease (NTD) caused by the protozoan parasite Trypanosoma cruzi and is primarily transmitted to humans by the feces of infected Triatominae insects during their blood meal. The disease affects 6-8 million people, mostly in Latin America countries, and kills more people in the region each year than any other parasite-born disease, including malaria. Moreover, patient numbers are currently increasing in non-endemic, developed countries, such as Australia, Japan, Canada, and the United States. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejmech.2018.11.062DOI Listing
November 2018

Design, synthesis, in silico pharmacokinetics prediction and biological evaluation of 1,4-dihydroindeno[1,2-c]pyrazole chalcone as EGFR /Akt pathway inhibitors.

Eur J Med Chem 2018 Dec 8;163:636-648. Epub 2018 Dec 8.

Organic Synthesis and Process Chemistry Division, CSIR-Indian Institute of Chemical Technology, Hyderabad 500007, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India. Electronic address:

In an attempt to develop potent and selective anticancer agents, a series of 15 conjugates of 1,4-dihydroindeno[1,2-c]pyrazole chalcone (12a-o) were designed, synthesized and evaluated for their antiproliferative activity against MCF7, A549, MDA-MB-231, HCT116 and SKBR3 human cancer cell lines. Among them, 12h, 12l and 12m showed IC values: 3.82, 5. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejmech.2018.12.011DOI Listing
December 2018

Discovery of novel quaternary ammonium compounds based on quinuclidine-3-ol as new potential antimicrobial candidates.

Eur J Med Chem 2018 Dec 12;163:626-635. Epub 2018 Dec 12.

University of Split, Faculty of Science, Department of Chemistry, R. Boškovića 33, 21 000, Split, Croatia. Electronic address:

Quaternary ammonium compounds (QACs) are amphiphilic molecules displaying a broad-spectrum of antibacterial activity. QACs are commonly used antiseptics in industrial, home and hospital settings. Given the emergence of the QAC-resistant bacteria, there is an urgent need to design new QACs with good antimicrobial activity, able to escape the host resistance mechanism. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejmech.2018.12.023DOI Listing
December 2018

In vitro exposure to 2,2',4,4'-tetrabromodiphenyl ether (PBDE-47) impairs innate inflammatory response.

Chemosphere 2018 Dec 11;219:845-854. Epub 2018 Dec 11.

Istituto di Biomedicina e di Immunologia Molecolare del Consiglio Nazionale delle Ricerche, Palermo, Italy. Electronic address:

Polybrominated diphenyl ethers (PBDEs) are persistent organic pollutants that are added to numerous products to prevent accidental fires. PBDEs are present in the environment and they bio-accumulate in human and animal tissues. Recently, their presence has been correlated to several pathologies but little is known about their effect on the human innate immune system activity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chemosphere.2018.12.082DOI Listing
December 2018

Preparation, characterization and application of anti-human OX40 ligand (OX40L) monoclonal antibodies and establishment of a sandwich ELISA for autoimmune diseases detection.

Int Immunopharmacol 2018 Dec 15;67:260-267. Epub 2018 Dec 15.

Jiangsu Key Laboratory of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou, China. Electronic address:

OX40L (CD252, TNFSF4), a type II transmembrane protein which like other tumor necrosis factor ligands, involved in the costimulation and differentiation of T cells, functions as a positive signal in immune response. To investigate the biological function of soluble OX40L (sOX40L), three functional anti-OX40L monoclonal antibodies (mAbs) 3D2, 3F7 and 2H3 were obtained by hybridoma technology. Besides, specificity of the mAbs was further demonstrated by ELISA, Western blot and Immunofluorescence experiments. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.intimp.2018.11.053DOI Listing
December 2018

Semaphoring 4D is required for the induction of antioxidant stress and anti-inflammatory effects of dihydromyricetin in colon cancer.

Int Immunopharmacol 2018 Dec 15;67:220-230. Epub 2018 Dec 15.

Department of Oncology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China. Electronic address:

Semaphorin 4D (Sema4D) has been involved in cancer progression, the expression of which is associated with the poor clinical outcomes of some cancer patients. Dihydromyricetin (DMY) has antitumor potentials for different types of human cancer cells. However, the pharmacological effects of DMY on colon cancer (CC) or the regulatory effects of Sema4D on this process remain largely unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.intimp.2018.12.025DOI Listing
December 2018

Endometrium cytokine profiles are altered following ovarian stimulation but almost not in subsequent hormone replacement cycles.

Cytokine 2018 Dec 15;114:6-10. Epub 2018 Dec 15.

Reproductive Medicine Center of Ruijin Hospital Affilited to Medical School of Shanghai Jiaotong University, Shanghai, China.

Purpose: To investigate the impact of ovarian stimulation and hormone replacement treatment on key regulatory cytokines in endometrial secretion during endometrium implantation window.

Methods: Fifty-six patients undergoing ovarian stimulation (OS) with gonadotropin releasing hormone antagonist and frozen embryo transfer with hormone replacement treatment (HRT) were recruited. Endometrial secretion aspiration was performed repeatedly during implantation window in natural, OS and HRT cycles of every patient. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cyto.2018.11.002DOI Listing
December 2018

Coupling the dynamics of diffused gases and microbial growth in modified atmosphere packaging.

Int J Food Microbiol 2018 Dec 8;292:31-38. Epub 2018 Dec 8.

Faculty of Health Sciences, Department of Food Sciences and Nutrition, University of Malta, Msida MSD 2080, Malta; Centre of Molecular Medicine and Biobanking, University of Malta, Msida MSD 2080, Malta. Electronic address:

Coupling microbial dynamics with the complete dynamics of the packaging gases is still a challenge. In this work the microbial growth kinetic parameters for Pseudomonas and Lactic Acid Bacteria (LAB) in MAP are identified based on accurate estimation of diffusivity of gases and parameter scaled sensitivity approaches. The microbial dynamics are also compared with those estimated based on partial pressure measurement. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijfoodmicro.2018.11.027DOI Listing
December 2018

Scanningless and continuous 3D bioprinting of human tissues with decellularized extracellular matrix.

Biomaterials 2018 Dec 10;194:1-13. Epub 2018 Dec 10.

Department of NanoEngineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA; Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA; Materials Science and Engineering Program, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA; Chemical Engineering Program, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA. Electronic address:

Decellularized extracellular matrices (dECMs) have demonstrated excellent utility as bioscaffolds in recapitulating the complex biochemical microenvironment, however, their use as bioinks in 3D bioprinting to generate functional biomimetic tissues has been limited by their printability and lack of tunable physical properties. Here, we describe a method to produce photocrosslinkable tissue-specific dECM bioinks for fabricating patient-specific tissues with high control over complex microarchitecture and mechanical properties using a digital light processing (DLP)-based scanningless and continuous 3D bioprinter. We demonstrated that tissue-matched dECM bioinks provided a conducive environment for maintaining high viability and maturation of human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes and hepatocytes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biomaterials.2018.12.009DOI Listing
December 2018

Next-generation sequencing reveals new insights about gene usage and CDR-H3 composition in the horse antibody repertoire.

Mol Immunol 2018 Dec 15;105:251-259. Epub 2018 Dec 15.

Laboratory of Synthetic Biology and Biomimetics, Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas - ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. Electronic address:

Horse serum antibodies have been used for greater than a century for the treatment and prophylaxis of infectious diseases and envenomations. Little is known, however, about the immunogenetic diversity that produces horse serum antibodies. Here, we employed next-generation sequencing for a first-in-kind comprehensive analysis of the equine B-cell repertoire. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.molimm.2018.11.017DOI Listing
December 2018

Breast cancer detection using targeted plasma metabolomics.

J Chromatogr B Analyt Technol Biomed Life Sci 2018 Dec 3;1105:26-37. Epub 2018 Dec 3.

Arizona Metabolomics Laboratory, College of Health Solutions, Arizona State University, Scottsdale, AZ 85259, USA. Electronic address:

Breast cancer (BC) is a major cause of human morbidity and mortality, especially among women. Despite the important role of metabolism in the molecular pathogenesis of cancer, robust metabolic markers to enable enhanced screening and disease monitoring of BC are still critically needed. In this study, a targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) metabolic profiling approach is presented for the identification of metabolic marker candidates that could enable highly sensitive and specific detection of all-stage as well as early-stage BC. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jchromb.2018.11.029DOI Listing
December 2018

NGF Increases FGF2 Expression and Promotes Endothelial Cell Migration and Tube Formation Through PI3K/Akt and ERK/MAPK Pathways in Human Chondrocytes.

Osteoarthritis Cartilage 2018 Dec 15. Epub 2018 Dec 15.

Department of Orthopaedic Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Jiefang Road 88#, Hangzhou 310009, China; Orthopaedics Research Institute, Zhejiang University, Jiefang Road 88#, Hangzhou, 310009, China. Electronic address:

Objective: Vascular invasion is observed at the osteochondral junction in osteoarthritis (OA). Nerve growth factor (NGF) as an angiogenic factor is expressed in OA. This study is to investigate the effects of NGF on angiogenesis in vitro in human chondrocytes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.joca.2018.12.007DOI Listing
December 2018

Characterization of the thickness of the tear film lipid layer using high resolution microscopy.

Ocul Surf 2018 Dec 15. Epub 2018 Dec 15.

The University of Alabama at Birmingham, School of Optometry, Department of Optometry & Vision Science, 1716 University Blvd, Birmingham, AL, 35223, USA. Electronic address:

Interferometry is an optical technique that have been used to quantify the lipid layer of the precorneal tear film, and to investigate the relationship between lipid layer thickness and tear film evaporation. However, the relationship between lipid layer thickness and the rate of evaporation is far from consistent. One possible reason is the inherent limit of contemporary interferometric systems, which employ objectives with relatively long depth of focus (DOF) (>15 μm or more), which tend to collect excessive extra-planar noise. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jtos.2018.12.003DOI Listing
December 2018

Potential effects of hepatitis E virus infection in swine on public health in China.

Infect Genet Evol 2018 Dec 15. Epub 2018 Dec 15.

State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, PR China. Electronic address:

Hepatitis E virus (HEV), a zoonotic pathogen, is the main cause of acute hepatitis worldwide. Swine serves as the main reservoir, and its infection is mainly transmitted via fecal-oral route. Due to huge consumption of pork in China, close human-swine interactions at pig farms likely contribute to high risk in zoonotic transmission of HEV. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.meegid.2018.12.017DOI Listing
December 2018

Fear but not social behaviour is affected by a polymorphism in the 5'-flanking region of the serotonin transporter (5-HTT) gene in adult hens.

Behav Brain Res 2018 Dec 15. Epub 2018 Dec 15.

Institute of Animal Welfare and Animal Husbandry, Friedrich-Loeffler-Institut, Dörnbergstraße 25/27, 29223 Celle, Germany. Electronic address:

The serotonin transporter gene (5-HTT) is involved in the regulation of the neural serotonin. Polymorphisms in the 5-HTT gene have been described in many species to be involved in physiological processes and emotions. A functional polymorphism in the 5´-flanking region of the 5-HTT gene is known from chickens, with a deletion-allele (D), which is associated with an increased 5-HTT expression, in comparison to the wild-type-allele (W). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbr.2018.12.029DOI Listing
December 2018

Food vs money? Effects of hunger on mood and behavioral reactivity to reward in anorexia nervosa.

Appetite 2018 Dec 15. Epub 2018 Dec 15.

Unit of Clinical and Health Psychology, University of Fribourg, Fribourg, Switzerland.

Background: Previous studies using neuroimaging and behavioral measures reported altered reward processing in anorexia nervosa (AN). In addition, anhedonia states are frequently reported in AN, potentially due to the physiological stress produced by the permanent starvation. We investigated the effect of fasting and satiety on mood and time reactions to monetary rewards in AN patients and healthy controls. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.appet.2018.12.017DOI Listing
December 2018

Downregulation of TIMP2 attenuates sepsis-induced AKI through the NF-κb pathway.

Biochim Biophys Acta Mol Basis Dis 2018 Dec 15. Epub 2018 Dec 15.

Dept. of Critical Care Medicine, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei, China; Center of Critical Care Nephrology, Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA. Electronic address:

Acute kidney injury (AKI) is a frequent complication of sepsis and contributes to increased morbidity and mortality. Urinary tissue inhibitor of metalloproteinases-2 (TIMP2) has been recently recognized as an early biomarker to predict AKI in critically ill patients. However, the biological functions of TIMP2 remain largely unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbadis.2018.10.041DOI Listing
December 2018

Pharmacokinetics of intra-articular vitamin D analogue calcipotriol in sheep and metabolism in human synovial and mesenchymal stromal cells.

J Steroid Biochem Mol Biol 2018 Dec 15. Epub 2018 Dec 15.

Cancer Research and Translational Medicine Research Unit, Medical Research Center Oulu, Oulu University Hospital and University of Oulu, P.O.Box 8000 FI-90014, University of Oulu, Oulu, Finland; Rheumatology Unit, Department of Medicine, Oulu University Hospital and University of Oulu, MRC, Oulu, P.O. Box 10, 90029 OYS, Finland. Electronic address:

Calcipotriol (MC903) is a side chain analogue of the biologically active 1,25-dihydroxyvitamin D [1,25(OH)D]. Due to its anti-inflammatory and anti-proliferative effects on stromal cells, calcipotriol is a promising candidate for the local treatment of arthritis. In this preliminary work, we studied the pharmacokinetics and safety of calcipotriol after an IV (0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jsbmb.2018.12.006DOI Listing
December 2018

How reliable are in vitro IC values? Values vary with cytotoxicity assays in human glioblastoma cells.

Toxicol Lett 2018 Dec 15. Epub 2018 Dec 15.

School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen, UK. Electronic address:

Increasing evidence shows that discrepancies exist among in vitro cytotoxicity methods resulting in unreliable drug toxicity profiles. This is particularly criticial for cell lines such as gliomas which are histologically and genetically heterogeneous. The high level of variation in these cells makes comparative analysis difficult and is a severe limitation for the usefulness of high-throughput screening methods. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.toxlet.2018.12.004DOI Listing
December 2018

Parathyroid hormone-stimulation of Runx2 during osteoblast differentiation via the regulation of lnc-SUPT3H-1:16 (RUNX2-AS1:32) and miR-6797-5p.

Biochimie 2018 Dec 15. Epub 2018 Dec 15.

Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India. Electronic address:

Parathyroid hormone (PTH) acts as a regulator of calcium homeostasis and bone remodeling. Runx2, an essential transcription factor in bone, is required for osteoblast differentiation. Noncoding RNAs such as long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) play crucial roles in regulating gene expression in osteoblasts. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2018.12.006DOI Listing
December 2018
1 Read

Behavioral, cognitive and histological changes following neonatal anoxia: male and female rats' differences at adolescent age.

Int J Dev Neurosci 2018 Dec 15. Epub 2018 Dec 15.

Neurosciences Laboratory, Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Av. Professor Lineu Prestes, 2415, 05508-900, São Paulo, SP, Brazil. Electronic address:

Neonatal anoxia induces long-term brain injury that may underlie neurobehavioral deficits at adolescence. Neonatal anoxia, induced by exposure of 30-hour old pups to 100% nitrogen, represents a non-invasive and global stimulus, which simulates clinical conditions of human pre-term babies (around 6 gestational months). Previous studies showed that neonatal anoxia induced impairments of spatial memory and altered anxiety-like behaviors in male rats tested at adult age. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijdevneu.2018.12.002DOI Listing
December 2018

Genotoxic effects of the cyanobacterial pentapeptide nodularin in HepG2 cells.

Food Chem Toxicol 2018 Dec 15. Epub 2018 Dec 15.

National Institute of Biology, Department of Genetic Toxicology and Cancer Biology, Večna pot 111, 1000, Ljubljana, Slovenia. Electronic address:

The cyanobacterial pentapeptide nodularin (NOD), mainly produced by genus Nodularia, is a potent inhibitor of protein phosphatases PPA1 and PPA2, and causes animal mortality. The few studies available indicate that NOD is a potential non-genotoxic carcinogen. In the present study we evaluated NOD (0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.fct.2018.12.019DOI Listing
December 2018

Ketone body modulation of ligand-gated ion channels.

Neuropharmacology 2018 Dec 15. Epub 2018 Dec 15.

Department of Neuroscience, Division of Pharmacology and Toxicology, Waggoner Center for Alcohol and Addiction Research, Institutes for Neuroscience and Cell and Molecular Biology, University of Texas at Austin, Austin, TX, 78712, USA. Electronic address:

Ketogenesis is a metabolic process wherein ketone bodies are produced from the breakdown of fatty acids. In humans, fatty acid catabolism results in the production of acetyl-CoA which can then be used to synthesize three ketone bodies: acetoacetate, acetone, and β-hydroxybutyrate. Ketogenesis occurs at a higher rate in situations of low blood glucose, such as during fasting, heavy alcohol consumption, and in situations of low insulin, as well as in individuals who follow a 'ketogenic diet' consisting of low carbohydrate and high fat intake. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.neuropharm.2018.12.013DOI Listing
December 2018

Decoding systems biology of plant stress for sustainable agriculture development and optimized food production.

Prog Biophys Mol Biol 2018 Dec 15. Epub 2018 Dec 15.

National Centre for Biological Sciences (TIFR), GKVK Campus, Bangalore, 560065, India. Electronic address:

Plants are essential facilitators of human life on planet earth. Plants play a critical functional role in mediating the quality of air, availability of food and the sustainability of agricultural resources. However, plants are in constant interaction with its environment and often hampered by various types of stresses like biotic and abiotic ones. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.pbiomolbio.2018.12.002DOI Listing
December 2018

Monkey Memory: Rehearsal Emerges for Novel Images When Familiarity Cues Fade.

Authors:
Michael J Beran

Curr Biol 2018 Dec;28(24):R1399-R1400

Department of Psychology and Language Research Center, Georgia State University, Atlanta, GA 30302, USA. Electronic address:

It was thought that, when monkeys use familiarity cues to aid recognition memory, they do not engage working memory. A new study shows that, when the value of those familiarity cues is attenuated, monkeys rehearse novel images like familiar ones, a striking parallel with human working memory. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cub.2018.10.061DOI Listing
December 2018

Drosophila: Where the Wild Flies Are.

Curr Biol 2018 Dec;28(24):R1382-R1384

Department of Integrative Biology, University of California, Berkeley, 3040 Valley Life Sciences Building, Berkeley, CA 94720, USA. Electronic address:

Drosophila melanogaster is a human commensal and dietary generalist. A new study in its ancestral range in Africa finds that wild Drosophila melanogaster are specialists on marula fruit - fruits cached in caves by Pleistocene humans. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cub.2018.11.034DOI Listing
December 2018

Stroking modulates noxious-evoked brain activity in human infants.

Curr Biol 2018 Dec;28(24):R1380-R1381

Department of Paediatrics, University of Oxford, Oxford, OX3 9DU, UK. Electronic address:

A subclass of C fibre sensory neurons found in hairy skin are activated by gentle touch [1] and respond optimally to stroking at ∼1-10 cm/s, serving a protective function by promoting affiliative behaviours. In adult humans, stimulation of these C-tactile (CT) afferents is pleasant, and can reduce pain perception [2]. Touch-based techniques, such as infant massage and kangaroo care, are designed to comfort infants during procedures, and a modest reduction in pain-related behavioural and physiological responses has been observed in some studies [3]. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cub.2018.11.014DOI Listing
December 2018

Metacognitive Failure as a Feature of Those Holding Radical Beliefs.

Curr Biol 2018 Dec;28(24):4014-4021.e8

Wellcome Centre for Human Neuroimaging, University College London, London WC1N 3BG, UK; Max Planck University College London Centre for Computational Psychiatry and Ageing Research, London WC1B 5EH, UK. Electronic address:

Widening polarization about political, religious, and scientific issues threatens open societies, leading to entrenchment of beliefs, reduced mutual understanding, and a pervasive negativity surrounding the very idea of consensus [1, 2]. Such radicalization has been linked to systematic differences in the certainty with which people adhere to particular beliefs [3-6]. However, the drivers of unjustified certainty in radicals are rarely considered from the perspective of models of metacognition, and it remains unknown whether radicals show alterations in confidence bias (a tendency to publicly espouse higher confidence), metacognitive sensitivity (insight into the correctness of one's beliefs), or both [7]. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cub.2018.10.053DOI Listing
December 2018
  • Page 1 of 361845