18,084,474 results match your criteria Human Pathology [Journal]

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Infection of HeLa cells with Chlamydia trachomatis inhibits protein synthesis and causes multiple changes to host cell pathways.

Cell Microbiol 2018 Dec 14:e12993. Epub 2018 Dec 14.

Institute for Microbiology and Hygiene, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Germany.

The obligate intracellular bacterium Chlamydia trachomatis replicates in a cytosolic vacuole in human epithelial cells. Infection of human cells with C. trachomatis causes substantial changes to many host cell signalling pathways but the molecular basis of such influence is not well understood. Read More

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December 2018

Predictors of mucosal healing during induction therapy in patients with acute moderate-to-severe ulcerative colitis.

J Gastroenterol Hepatol 2018 Dec 14. Epub 2018 Dec 14.

Department of Gastroenterology and Hepatology, Tokyo Medical and Dental University, Tokyo, Japan.

Background And Aim: The treat-to-target strategy has emerged in ulcerative colitis management. Mucosal healing is the best target, albeit not in induction therapy of acute diseases as clinical conditions vary over a short duration. To determine the targets during induction therapy for acute ulcerative colitis, we identified markers to predict mucosal healing at 3 and 12 months of initiating the induction therapy. Read More

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December 2018

Cardiovascular and Metabolic Risk in Women in the First Year Postpartum: Allostatic Load as a Function of Race, Ethnicity, and Poverty Status.

Am J Perinatol 2018 Dec 14. Epub 2018 Dec 14.

Department of Internal Medicine, UCLA Geffen School of Medicine, Los Angeles, California.

Objective:  Allostatic load (AL) represents multisystem physiological "wear-and-tear" reflecting emerging chronic disease risk. We assessed AL during the first year postpartum in a diverse community sample with known health disparities.

Methods:  The Shriver National Institute for Child Health and Human Development Community Child Health Network enrolled 2,448 predominantly low-income African-American, Latina, and White women immediately after delivery of liveborn infants at ≥20 weeks' gestation, following them over time with interviews, clinical measures, and biomarkers. Read More

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December 2018

Menthol cigarettes, tobacco dependence, and smoking persistence: The need to examine enhanced cognitive functioning as a neuropsychological mechanism.

Nicotine Tob Res 2018 Dec 14. Epub 2018 Dec 14.

Department of Preventive Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA.

Introduction: Despite the overall decline in the prevalence of cigarette use in the United States, menthol cigarette use among smokers is rising, and evidence shows that it may lead to more detrimental effects on public health than regular cigarette use. One of the mechanisms by which nicotine sustains tobacco use and dependence is due to its cognitive enhancing properties, and basic science literature suggests that menthol may also enhance nicotine's acute effect on cognition.

Aims And Methods: The purpose of this review is to suggest that the cognitive enhancing effects of menthol may be a potentially important neuropsychological mechanism that has yet to be examined. Read More

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December 2018

Calreticulin regulates syncytialization through control of the synthesis and transportation of E-cadherin in BeWo cells.

Endocrinology 2018 Dec 14. Epub 2018 Dec 14.

Department of Obstetrics and Gynecology, Wakayama Medical University.

During placental development, mononuclear cytotrophoblasts differentiate and fuse to syncytiotrophoblasts (STB) to form syncytia, which secrete human chorionic gonadotropin (hCG). Decreased maternal perfusion of the placenta, which leads to placental dysfunction, induces changes in trophoblast syncytialization. Our previous study showed that calreticulin (CRT), a Ca2+-binding molecular chaperone found in the endoplasmic reticulum, is expressed in the human placenta and is involved in regulating extravillous trophoblast invasion, although its role in villous trophoblasts remains unclear. Read More

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December 2018

Network localization of heterogeneous neuroimaging findings.

Brain 2018 Dec 14. Epub 2018 Dec 14.

Berenson-Allen Center for Noninvasive Brain Stimulation, Department of Neurology, Beth Israel Deaconess Medical Center, Harvard Medical Center, Boston, MA, USA.

Studies of the same disease often implicate different brain regions, contributing to a perceived reproducibility crisis in neuroimaging. Here, we leverage the normative human brain connectome to test whether seemingly heterogeneous neuroimaging findings localize to connected brain networks. We use neurodegenerative disease, and specifically Alzheimer's disease, as our example as it is one of the diseases that has been studied the most using neuroimaging. Read More

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December 2018

Staphylococcus aureus Decolonization of Mice With Monoclonal Antibody Neutralizing Protein A.

J Infect Dis 2018 Dec 15. Epub 2018 Dec 15.

Department of Microbiology, University of Chicago, Illinois.

Staphylococcusaureus persistently colonizes the nasopharynx of about one-third of the human population, a key risk factor for community- and hospital-acquired invasive infections. Current strategies for S. aureus decolonization include topical and systemic administration of antibiotics, which is associated with selection for antibiotic resistance and posttreatment recolonization. Read More

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December 2018

Lower skeletal muscle mass at admission independently predicts falls and mortality three months post-discharge in hospitalised older patients.

J Gerontol A Biol Sci Med Sci 2018 Dec 14. Epub 2018 Dec 14.

Department of Medicine and Aged Care, @AgeMelbourne, The Royal Melbourne Hospital, The University of Melbourne, Victoria, Australia.

Background: Approximately 10% of older adults is annually admitted to a hospital. Hospitalisation is associated with a higher risk of falls and mortality after discharge. This study aimed to identify predictors at admission for falls and mortality three months post-discharge in hospitalised older patients. Read More

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December 2018

Anomalous Bodily Maps of Emotions in Schizophrenia.

Schizophr Bull 2018 Dec 14. Epub 2018 Dec 14.

Department of Psychology, Vanderbilt University, Nashville, TN.

Objective: Embodied emotions arise from interoceptive and somatosensory processes, and are essential to the development of a stable sense of self. Emotional embodiment is therefore inherently interwoven with our sense of bodily self-awareness, and allows us to navigate complex social situations. Given that the core feature of schizophrenia (SZ) is characterized by the presence of bodily self-disturbances and social-emotional deficits, we hypothesized that embodiment of emotion would be disrupted in SZ. Read More

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December 2018
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CRISPR-C: circularization of genes and chromosome by CRISPR in human cells.

Nucleic Acids Res 2018 Dec;46(22):e131

Department of Biomedicine, Aarhus University, Denmark.

Extrachromosomal circular DNA (eccDNA) and ring chromosomes are genetic alterations found in humans with genetic disorders. However, there is a lack of genetic engineering tools to recapitulate and study the biogenesis of eccDNAs. Here, we created a dual-fluorescence biosensor cassette, which upon the delivery of pairs of CRISPR/Cas9 guide RNAs, CRISPR-C, allows us to study the biogenesis of a specific fluorophore expressing eccDNA in human cells. Read More

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December 2018

Workplace Exposure to Nanoparticles during Thermal Spraying of Ceramic Coatings.

Ann Work Expo Health 2018 Dec 14. Epub 2018 Dec 14.

Institute of Environmental Assessment and Water Research (IDAEA-CSIC), Barcelona, Spain.

Thermal spraying is widely used for industrial-scale application of ceramic coatings onto metallic surfaces. The particular process has implications for occupational health, as the high energy process generates high emissions of metal-bearing nanoparticles. Emissions and their impact on exposure were characterized during thermal spraying in a work environment, by monitoring size-resolved number and mass concentrations, lung-deposited surface area, particle morphology, and chemical composition. Read More

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December 2018

The serogroup B meningococcal vaccine Bexsero elicits antibodies to Neisseria gonorrhoeae.

Clin Infect Dis 2018 Dec 14. Epub 2018 Dec 14.

Institute for Glycomics, Griffith University, Gold Coast, Australia.

Background: Neisseria gonorrhoeae and Neisseria meningitidis are closely related bacteria that cause a significant global burden of disease. Control of gonorrhoea is becoming increasingly difficult due to widespread antibiotic resistance. While vaccines are routinely used for N. Read More

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December 2018

Functionality and cross-regulation of the four SprG/SprF type I toxin-antitoxin systems in Staphylococcus aureus.

Nucleic Acids Res 2018 Dec 15. Epub 2018 Dec 15.

Université de Rennes 1, Inserm, BRM (Bacterial Regulatory RNAs and Medicine) UMR_S 1230, 35000 Rennes, France.

Toxin-antitoxin (TA) systems are ubiquitous among bacteria, frequently expressed in multiple copies, and important for functions such as antibiotic resistance and persistence. Type I TA systems are composed of a stable toxic peptide whose expression is repressed by an unstable RNA antitoxin. Here, we investigated the functionalities, regulation, and possible cross-talk between three core genome copies of the pathogenicity island-encoded 'sprG1/sprF1' type I TA system in the human pathogen Staphylococcus aureus. Read More

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December 2018

Cytological and molecular aspects of the ageing sperm.

Hum Reprod 2018 Dec 14. Epub 2018 Dec 14.

Laboratory of Seminology - Sperm Bank 'Loredana Gandini' Department of Experimental Medicine, 'Sapienza' University of Rome, Italy.

Study Question: Is ageing associated with a decline in semen quality and molecular changes to human sperm?

Summary Answer: Semen quality declines with advancing age and characteristic molecular changes take place during the ageing process, including increased sperm DNA damage, altered sperm protamination and altered seminal plasma miRNA profile.

What Is Known Already: During ageing, the reproductive system is exposed to physiological changes and potentially damaging factors that may impair testicular function. Reactive oxygen species (ROS) can induce errors during DNA replication, transcription or post-transcriptional events (fragmentation, chromatin condensation abnormalities and protamine expression defects). Read More

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December 2018

Networking in eHealth research: results of the IDRC SEARCH program evaluation.

J Public Health (Oxf) 2018 Dec;40(suppl_2):ii12-ii15

Department of Health Research Methods, Evidence and Impact, McMaster University, Hamilton, Ontario, Canada.

Background: The IDRC 'Strengthening Equity through Applied Research Capacity building in eHealth' (SEARCH) funded seven research projects in Bangladesh, Burkina Faso, Ethiopia, Kenya, Lebanon, Peru and Vietnam that sought to answer questions or test solutions related to the use of Internet or mobile phone technology in strengthening health systems. The evaluation accompanied these projects over two years to answer, among others, the question how cross-grant learning interactions influenced project outcomes.

Methods: The evaluation team conducted repeated interviews and on-line questionnaire surveys with the research teams and analysed the information exchanges among researchers on a SharePoint site established by IDRC. Read More

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December 2018

Significant inhibition of photosynthesis and respiration in leaves of Cucumis sativus L. by oxybenzone, an active ingredient in sunscreen.

Chemosphere 2018 Dec 3;219:456-462. Epub 2018 Dec 3.

State Key Laboratory of Crop Biology, China; College of Life Sciences, Shandong Agricultural University, China. Electronic address:

Oxybenzone (OBZ), an active ingredient in most sunscreens, was recently shown to be toxic to humans, corals and other animals. This study is the first to demonstrate that OBZ can significantly inhibit photosynthesis and respiration in the leaves of a higher plant, cucumber. An OBZ suspension content as low as 0. Read More

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December 2018
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Biomechanical testing of a polycarbonate-urethane-based dynamic instrumentation system under physiological conditions.

Clin Biomech (Bristol, Avon) 2018 Dec 6;61:112-119. Epub 2018 Dec 6.

University of Cologne, Centre for Orthopaedic and Trauma Surgery, Germany.

Background: Posterior dynamic stabilization systems are developed to maintain the healthy biomechanics of the spine while providing stabilization. Numerous dynamic systems incorporate polycarbonate urethane with temperature- and moisture-dependent material properties. In the underlying study, a novel test rig is used to evaluate the biomechanical performance of a system containing polycarbonate urethane. Read More

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December 2018

Common genetic variants contribute to incomplete penetrance: evidence from cancer-free BRCA1 mutation carriers.

Eur J Cancer 2018 Dec 11;107:68-78. Epub 2018 Dec 11.

Department of Genetics, Cell Biology and Anatomy, College of Medicine, USA; Cancer Centre and Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Taipa, Macau, SAR, China. Electronic address:

Purpose: The presence of pathogenic germline mutation in BRCA1 gene is considered as the most penetrant genetic predisposition for breast cancer. However, a portion of BRCA1 mutation carriers never develops breast cancer throughout their lifetime. This phenomenon is called incomplete penetrance. Read More

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December 2018

Influence of drivers' psychological risk profiles on the effectiveness of traffic calming measures.

Accid Anal Prev 2018 Dec 11;123:243-255. Epub 2018 Dec 11.

Department of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa, Via Paradisa 2, 56126, Pisa, Italy.

Road traffic injuries represent a serious public health problem and are one of leading cause of death, injury and disability around the world. Road accidents are often caused by an accumulation of factors; however, drivers appear to be by far the most decisive one. The driver's behaviour is complex and depends on reflex (or involuntary) and voluntary driving actions. Read More

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December 2018

A prediction method of speed-dependent walking patterns for healthy individuals.

Gait Posture 2018 Dec 5;68:280-284. Epub 2018 Dec 5.

Federal University of ABC, Neuroscience and Biomedical Engineering Programs, São Bernardo do Campo, São Paulo, Brazil. Electronic address:

Background: Gait speed is one of the main biomechanical determinants of human movement patterns. However, in clinical gait analysis, the effect of gait speed is generally not considered, and people with disabilities are usually compared with able-bodied individuals even though disabled people tend to walk slower.

Research Questions: This study proposes a simple way to predict the gait pattern of healthy individuals at a specific speed. Read More

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December 2018

Using tDCS to facilitate motor learning in speech production: The role of timing.

Cortex 2018 Nov 19;111:274-285. Epub 2018 Nov 19.

Burke Medical Research Institute, Edith Cowan University and Weill Cornell Medicine, White Plains, NY, USA; Moss Rehabilitation Research Institute, Philadelphia, PA, USA.

There exists debate regarding the extent to which transcranial direct current stimulation (tDCS) can affect or enhance human behavior. Here, we examined a previously unexplored domain: speech motor learning. We investigated whether speech motor learning in unimpaired participants can be enhanced using a single-session tDCS experiment, and investigated whether the timing of tDCS relative to a behavioral task affected performance. Read More

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November 2018

Direct potentiation of NK cell cytotoxicity by 8-azaguanine with potential antineoplastic activity.

Int Immunopharmacol 2018 Dec 11;67:152-159. Epub 2018 Dec 11.

Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea; Microbiology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea; Stem Cell Immunomodulation Research Center (SCIRC), Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea. Electronic address:

This study identified 8-azaguanine (8-AG) as a novel immunomodulatory drug (IMiD) through a high-throughput screen of the Preswick Chemical Library in a model of human NK cell cytotoxicity against blood cancer cells. 8-AG, originally developed as an antineoplastic agent, significantly increased the cytotoxicity of NK cells and was superior in this activity to previously known IMiDs, such as fluoxetine and amphotericin B, identified from the same library. IFN-γ expression was also slightly increased by 8-AG. Read More

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December 2018

Recombinant human interleukin-1 receptor antagonist treatment protects rats from myocardial ischemia-reperfusion injury.

Biomed Pharmacother 2018 Dec 11;111:1-5. Epub 2018 Dec 11.

Department of Cardiology, Rui Jin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 20025, PR China. Electronic address:

The interleukin-1 (IL-1) signaling pathway plays a vital role in multiple mechanisms associated with myocardial ischemia-reperfusion (I/R) injury, including inflammation and apoptosis. An IL-1 receptor antagonist (IL-1Ra) can block IL-1 by competitive binding to the IL-1 receptor type I (IL-1RI) and thus may provide a cardioprotective effect. In the present investigation, we determined whether exogenous administration of recombinant human IL-1Ra (rhIL-1Ra) provides a protective role against myocardial I/R injury. Read More

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December 2018

A novel pro-apoptotic role of zinc octacarboxyphthalocyanine in melanoma me45 cancer cell's photodynamic therapy (PDT).

J Photochem Photobiol B 2018 Dec 5;190:146-153. Epub 2018 Dec 5.

Biosystems Group, Institute of Automatic Control, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland; Biotechnology Centre, Silesian University of Technology, Krzywoustego 8, 44-100 Gliwice, Poland. Electronic address:

Zn-based phthalocyanine acts as drug or photosensitizer in photodynamic therapy (PDT) for the treatment of cancer cells. The activated zinc octacarboxyphthalocyanine (ZnPcOC) reacts with oxygen, to generate reactive oxygen species for the damage of melanoma cancer cells, Me45. This in vitro study aimed at investigating the cytotoxic effects of different concentrations of ZnPcOC activated with a diode laser (λ = 685 nm) on Me45, and normal human fibroblast cells, NHDF. Read More

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December 2018

Deficits in rate of torque development are accompanied by activation failure in patients with knee osteoarthritis.

J Electromyogr Kinesiol 2018 Dec 4;44:94-100. Epub 2018 Dec 4.

Human Performance Lab, Schulthess Clinic, Zurich, Switzerland. Electronic address:

Aim of this study was to investigate if knee extensor maximal voluntary contraction (MVC) torque and rate of torque development (RTD) deficits are accompanied by neuromuscular activation failure in patients with knee osteoarthritis (KOA). Nineteen patients with unilateral KOA completed gradual MVCs, from which MVC torque, voluntary activation and maximal EMG activity were recorded, and explosive MVCs, from which RTD and rate of EMG rise were recorded. For gradual MVCs, MVC torque (-28%), voluntary activation (-6%) and maximal EMG activity (-30%) were lower on the involved than on the uninvolved side (p < 0. Read More

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December 2018

Synthesis, photodynamic activities, and cytotoxicity of new water-soluble cationic gallium(III) and zinc(II) phthalocyanines.

J Inorg Biochem 2018 Dec 5;192:7-16. Epub 2018 Dec 5.

Department of Chemistry, Graduate School of Science and Engineering, Shimane University, 1060 Nishikawatsu, Matsue 690-8504, Japan. Electronic address:

The cationic Ga(III) and Zn(II) phthalocyanines carrying N-methyl-pyridinium groups at eight peripheral β-positionshave been synthesized. These complexes are highly soluble in dimethyl sulfoxide (DMSO) and moderately soluble in water and phosphate buffered saline (PBS); both Ga(III)Cl and Zn(II) complexes have shown no aggregation in water up to 1.2 × 10 and 1. Read More

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December 2018

Near-infrared light control of bone regeneration with biodegradable photothermal osteoimplant.

Biomaterials 2018 Dec 10;193:1-11. Epub 2018 Dec 10.

Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China. Electronic address:

Mild heat stimulation can promote the restoration of bone defects but unfortunately, the delivery of exo-hyperthermy into human body is not efficient enough. In this study, mild heat-induced osteogenesis with high efficacy is demonstrated on an osteoimplant composed of black phosphorus nanosheets and poly(lactic-co-glycolic acid) (BPs@PLGA) with the participation of near-infrared (NIR) light irradiation. BPs@PLGA with only 0. Read More

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December 2018
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Antitumor effect of chondroitin AC lyase (PsPL8A) from Pedobacter saltans on melanoma and fibrosarcoma cell lines by in vitro analysis.

Pharmacol Rep 2018 Oct 25;71(1):167-174. Epub 2018 Oct 25.

Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, India. Electronic address:

Background: PGs are involved in cellular communication and cancer biology. The role of CS in melanoma and fibrosarcoma cell lines was explored by using chondroitin AC lyase (PsPL8A).

Methods: The proliferation of mouse fibroblast L929, human melanoma (SK-Mel 28) and fibrosarcoma (HT-1080) cell lines after treatment with chondroitin AC lyase (PsPL8A) was studied by MTT assay. Read More

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October 2018

An improved rat model for chronic inflammatory bowel disease.

Pharmacol Rep 2018 Oct 11;71(1):149-155. Epub 2018 Oct 11.

School of Health and Wellbeing, University of Southern Queensland, Toowoomba, QLD, Australia; Functional Foods Research Group, University of Southern Queensland, Toowoomba, QLD, Australia. Electronic address:

Background: Inflammatory bowel disease (IBD) is an important cause of chronic disability in humans.

Methods: We characterized a model of chronic IBD in young male Wistar rats by administering dextran sodium sulfate (DSS: 0%, 0.25%, 0. Read More

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October 2018

Fish antiviral tripartite motif (TRIM) proteins.

Fish Shellfish Immunol 2018 Dec 11. Epub 2018 Dec 11.

INRA, Virologie et Immunologie Moléculaires, Université Paris-Saclay, Jouy-en-Josas, France. Electronic address:

Tripartite motif (TRIM) family or RBCC proteins comprises characteristic zinc-binding domains (a RING (R), a B-box type 1 (B1) and a B-box type 2 (B2)) and coiled-coil (CC) domain followed by a C-terminus variable domain. There are about 80 different TRIM proteins in human, but more than 200 in zebrafish with several large gene expansions (ftr >70 genes; btr >30 genes; trim35 > 30 genes). Repertoires of trim genes in fish are variable across fishes, but they have been remarkably diversified independently in a number of species. Read More

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December 2018

Early-life exposure to the endocrine disruptor 17-α-ethinylestradiol induces delayed effects in adult brain, liver and ovotestis proteomes of a self-fertilizing fish.

J Proteomics 2018 Dec 11. Epub 2018 Dec 11.

Laboratory of Evolutionary and Adaptive Physiology - Institute of Life, Earth and Environment - University of Namur, 61 Rue de Bruxelles, B5000 Namur, Belgium.

Early-life represents a critically sensitive window to endocrine disrupting chemicals, potentially leading to long-term repercussions on the phenotype later in life. The mechanisms underlying this phenomenon, referred to as the Developmental Origins of Health and Disease (DOHaD), are still poorly understood. To gain molecular understanding of these effects, we exposed mangrove rivulus (Kryptolebias marmoratus) for 28 days post hatching (dph) to 4 and 120 ng/L 17-α-ethinylestradiol, a model xenoestrogen. Read More

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December 2018

Selection and characterization of protective anti-chikungunya virus single domain antibodies.

Mol Immunol 2018 Dec 11;105:190-197. Epub 2018 Dec 11.

US Naval Research Laboratory, Center for Biomolecular Science and Engineering, 4555 Overlook Ave SW, Washington, DC 20375, USA. Electronic address:

Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that causes an arthralgia febrile illness that has affected millions of people on three continents. Previously, neutralizing monoclonal antibodies that have prophylactic and therapeutic activity were found to remove virus in joint tissues, thereby reducing the severity of symptoms in mice and non-human primates. In this study, we sought to develop thermostable small recombinant antibodies against CHIKV for future diagnostic, prophylactic and therapeutic applications. Read More

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December 2018

Three monophyletic clusters in Retortamonas species isolated from vertebrates.

Parasitol Int 2018 Dec 11. Epub 2018 Dec 11.

Department of Parasitology, Graduate School of Medical Sciences, Kanazawa University, Kanazawa 920-8640, Japan. Electronic address:

Retortamonas spp. has been reported as an intestinal parasite among various host organisms, including humans; however, its intra-genus molecular diversity has not yet been elucidated. Haplotypes of the 18S small subunit ribosomal RNA locus (1836-1899 bp) of Retortamonas spp. Read More

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December 2018

Human DNA degradation assessment and male DNA detection by quantitative-PCR followed by high-resolution melting analysis.

Forensic Sci Int 2018 Nov 29;295:1-7. Epub 2018 Nov 29.

Centro de Referencia en Identificación Genética Humana de la Universidad de Buenos Aires, Cátedra de Genética Forense y Servicio de Huellas Digitales Genéticas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junin 956, CP 1113, Ciudad Autónoma de Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas-CONICET, Godoy Cruz 2290, CP 1425, Ciudad Autónoma de Buenos Aires, Argentina.

We developed a q-PCR technique that simultaneously evaluates the extent of degradation and determines the gender of a human DNA donor. QYDEG HRM is a triplex real-time PCR whose products are analysed by high-resolution melting (HRM). The system produces three amplicons: (1) transducin (beta)-like 1, Y-linked (TBL1Y) (84bp); (2) large-target sequence (DGlt) (244bp); and (3) small-target sequence (DGst) (152bp). Read More

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November 2018

Comparison and implementation of different control strategies for improving production of rHSA using Pichia pastoris.

J Biotechnol 2018 Dec 11. Epub 2018 Dec 11.

Department of Chemical Engineering, Indian Institute of Technology, Hauz Khas, New Delhi, India. Electronic address:

Recombinant protein production by various host organisms is strictly regulated, which is determined mainly by host cell genetics and maintenance of process variables within desirable limits in a reactor. Since the process dynamics typically exhibit nonlinear interactions amongst the critical process variables, the commonly employed controllers are suboptimal for control of most bioprocesses. In this article, we present the implementation of an augmented controller for improving the production of recombinant human serum albumin using Pichia pastoris. Read More

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December 2018

Research Update and Opportunity of Non-hormonal Male Contraception: Histone Demethylase KDM5B-Based Targeting.

Pharmacol Res 2018 Dec 11. Epub 2018 Dec 11.

Department of Animal Science & Technology and BET Research Institute, Chung-Ang University, Anseong, Gyeonggi-do, 17546, Republic of Korea. Electronic address:

With the continued increase in global human population, diverse contraception approaches have become increasingly essential, including non-hormonal male contraception. Non-hormonal approaches to contraception are very convenient; however, such options are limited because data regarding the identification and characterization of tissue/cell-specific targets and appropriate small molecule candidate contraceptives are lacking. Based on in-silico studies of genomics, transcriptomics, and proteomics, performed by mining datasets in PubMed, we first reviewed testis-, epididymis-, and germline cell-specific genes/proteins, with the aim of presenting evidence that many of these could become 'druggable' targets for the development of non-hormonal male contraceptives in the future. Read More

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December 2018

ER stress promotes antitumor effects in BRAFi/MEKi resistant human melanoma induced by natural compound 4-nerolidylcathecol (4-NC).

Pharmacol Res 2018 Dec 11. Epub 2018 Dec 11.

Skin Biology Group, Clinical Chemistry and Toxicology Department, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address:

Melanoma accounts for only 4% of malignant neoplasms of the skin, but is considered the most serious because it is highly deadly. Mutations in the MAPK (Ras-Raf-MEK-ERK) pathway is closely linked to the lack of control of cell proliferation. Especially in melanoma, this pathway has become a target for the development of oncogene-targeted therapies, such as the potent inhibitors of v-Raf murine sarcoma viral oncogene homolog B (BRAFi) and mitogen-activated protein kinase kinase (MEKi). Read More

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December 2018
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The T-type calcium channel antagonist, Z944, alters social behavior in Genetic Absence Epilepsy Rats from Strasbourg.

Behav Brain Res 2018 Dec 11. Epub 2018 Dec 11.

University of Saskatchewan, Department of Physiology, Saskatoon, SK, Canada.

Abnormalities in social behavior are a co-morbid symptom of idiopathic generalized epilepsies such as childhood absence epilepsy. The Genetic Absence Epilepsy Rats from Strasbourg (GAERS) model is a spontaneously occurring absence epilepsy phenotype closely correlated to that of human absence epilepsies. Similar to the human conditions, GAERS display social abnormalities. Read More

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December 2018

Carrier-free nanoparticles of cathepsin B-cleavable peptide-conjugated doxorubicin prodrug for cancer targeting therapy.

J Control Release 2018 Dec 11. Epub 2018 Dec 11.

Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea; KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, 02841, Republic of Korea. Electronic address:

Cancer nanomedicine using nanoparticle-based delivery systems has shown outstanding promise in recent decades for improving anticancer treatment. However, limited targeting efficiency, low drug loading efficiency and innate toxicity of nanoparticles have caused severe problems, leaving only a few available in the clinic. Here, we newly developed carrier-free nanoparticles of cathepsin B-cleavable peptide (Phe-Arg-Arg-Gly; FRRG)-conjugated doxorubicin (DOX) prodrug (FRRG-DOX) that formed a stable nanoparticle structure with an average diameter of 213 nm in aqueous condition. Read More

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December 2018

Evaluation of three advanced methodologies, COLD-PCR, microarray and ddPCR, for identifying the mutational status by liquid biopsies in metastatic colorectal cancer patients.

Clin Chim Acta 2018 Dec 11. Epub 2018 Dec 11.

Unit of Genomic for the Diagnosis of Human Pathologies, Division of Genetics and Cell Biology, IRCCS San Raffaele Scientific Institute, Via Olgettina 60, Milan 20132, Italy; Laboratory of Clinical Molecular Biology, IRCCS Ospedale San Raffaele, Milan, Italy; Università Vita-Salute San Raffaele, Milano, Italy.

A major effort has been focused on the detection of oncogenes' mutations in diverse types of clinical specimens including formalin-fixed and paraffin embedded tissues, presently the gold-standard samples, up to plasma, that constitute a noninvasive alternative source of tumor DNA. The reliable detection of mutations in circulating tumor DNA requires a high analytical sensitivity. Here, we applied three different highly sensitive methodologies (COLD-PCR, a microarray-based approach and the droplet digital PCR, ddPCR) to identify mutations in the plasma of 30 metastatic colorectal cancer patients previously genotyped on tissue biopsy. Read More

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December 2018

Self-Assembled GFFYK Peptide Hydrogel Enhances the Therapeutic Efficacy of Mesenchymal Stem Cells in a Mouse Hindlimb Ischemia Model.

Acta Biomater 2018 Dec 11. Epub 2018 Dec 11.

Nankai University School of Medicine, Tianjin 300071, China; The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, The College of Life Science, Tianjin 300071, China. Electronic address:

Mesenchymal stem cell (MSC) transplantation has emerged as a very promising strategy for the treatments of peripheral artery disease (PAD). However, MSC-based therapies are limited by low cell retention and survival rate in the ischemic zone. Small molecular (SM) hydrogels have shown attractive abilities to enhance the therapeutic effects of human MSCs via promoting their proliferation or maintaining their differentiation potential. Read More

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December 2018

Toll-like receptor 3 activation increases voluntary alcohol intake in C57BL/6J male mice.

Brain Behav Immun 2018 Dec 11. Epub 2018 Dec 11.

Waggoner Center for Alcoholism and Addiction Research, University of Texas at Austin, Austin, TX 78712, USA; Institute for Neuroscience, University of Texas at Austin, Austin, TX 78712, USA.

Many genes differentially expressed in brain tissue from human alcoholics and animals that have consumed large amounts of alcohol are components of the innate immune toll-like receptor (TLR) pathway. TLRs initiate inflammatory responses via two branches: [1] MyD88-dependent or [2] TRIF-dependent. All TLRs signal through MyD88 except TLR3. Read More

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December 2018

Clinico-pathological characteristics among South African women with breast cancer receiving anti-retroviral therapy for HIV.

Breast 2018 Dec 7;43:123-129. Epub 2018 Dec 7.

Non-Communicable Diseases Research Division, Wits Health Consortium (PTY) Ltd, 31 Princess of Wales Building, Johannesburg, 2193, South Africa; Department of Medicine, University of Witwatersrand Faculty of Health Sciences, 7 York Road Johannesburg, South Africa.

Purpose: Breast cancer is the most common cancer in women and a leading cause of cancer-related mortality worldwide. South Africa has the largest global burden of HIV infection and the largest anti-retroviral treatment (ART) program. This study aimed to analyse the association of HIV and ART use with breast cancer clinico-pathological characteristics. Read More

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December 2018

Tracing lake mixing and oxygenation regime using the Fe/Mn ratio in varved sediments: 2000 year-long record of human-induced changes from Lake Żabińskie (NE Poland).

Sci Total Environ 2018 Dec 6;657:585-596. Epub 2018 Dec 6.

Faculty of Oceanography and Geography, University of Gdansk, Poland.

This study explores the long-term drivers of changes in lake mixing regime and the causes of lacustrine anoxia in the 2000 year-long, varved sediment record from Lake Żabińskie in northeastern Poland. Annually resolved geochemical data (μXRF and CNS) and pollen data were used to find links between changes in catchment land use and lake mixing regime. Furthermore, we tested the applicability of the Fe/Mn ratio for the reconstruction of past water ventilation. Read More

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December 2018

Centennial records of cadmium and lead in NE China lake sediments.

Sci Total Environ 2018 Nov 28;657:548-557. Epub 2018 Nov 28.

EcoLab - Laboratoire Ecologie Fonctionnelle et Environnement, Université de Toulouse, CNRS, INPT, UPS, 31000 Toulouse, France.

Cores (15 to 40 cm of depth) were collected from 11 lakes of the Songnen Plain in northeastern China to reconstruct changes in potentially harmful trace element (PHTE) inputs as tracers of human activities. In each profile, most PHTE enrichment factors do not differ significantly from the pre-industrial values (EF < 1.5), except for Cd (EF = 2-5. Read More

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November 2018

Stromal PTEN Regulates Extracellular Matrix Organization in the Mammary Gland.

Neoplasia 2018 Dec 11;21(1):132-145. Epub 2018 Dec 11.

Department of Biomedical Engineering, College of Engineering, The Ohio State University, Columbus, OH 43210; The James Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210. Electronic address:

The organization of the extracellular matrix has a profound impact on cancer development and progression. The matrix becomes aligned throughout tumor progression, providing "highways" for tumor cell invasion. Aligned matrix is associated with breast density and is a negative prognostic factor in several cancers; however, the underlying mechanisms regulating this reorganization remain poorly understood. Read More

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December 2018

Expanding the Molecular Characterization of Thoracic Inflammatory Myofibroblastic Tumors beyond ALK gene rearrangements.

J Thorac Oncol 2018 Dec 11. Epub 2018 Dec 11.

Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY. Electronic address:

Background: Half of inflammatory myofibroblastic tumors (IMTs) regardless of anatomic location harbor ALK gene rearrangements and overexpress ALK protein. The wide application of next generation sequencing (NGS) and the clinical benefit to tyrosine kinase inhibitors have opened new opportunities for investigation of ALK-negative IMT.

Design: In this study we investigate a series of pediatric and adult thoracic IMT for abnormalities in a wide spectrum of actionable kinases, by applying a variety of molecular and NGS techniques, including FISH, targeted RNA sequencing and NanoString. Read More

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December 2018

Ribosomal protein eL42 contributes to the catalytic activity of the yeast ribosome at the elongation step of translation.

Biochimie 2018 Dec 11. Epub 2018 Dec 11.

Sorbonne Université, Campus Pierre et Marie Curie, Laboratoire "Enzymologie de l'ARN", SU-UR6; (Bâtiment B); Case courrier 60 - 4, Place Jussieu, F-75252, Paris Cedex 05, France.

The GGQ minidomain of the ribosomal protein eL42 was previously shown to contact the CCA-arm of P-site bound tRNA in human ribosome, indicating a possible involvement of the protein in the catalytic activity. Here, using Schizosaccharomyces pombe (S. pombe) cells, we demonstrate that the GGQ minidomain and neighboring region of eL42 is critical for the ribosomal function. Read More

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December 2018

TRPC channels mediated calcium entry is required for proliferation of human airway smooth muscle cells induced by nicotine-nAChR.

Biochimie 2018 Dec 11. Epub 2018 Dec 11.

Respiratory Medicine, Hunan Provincial People's Hospital, No. 61 Jiefang Xi Road, Changsha, 410219, Hunan, PR China; Institute of Respiratory Medicine, Changsha Medical College, No. 1501 Leifeng Road, Changsha, 410219, Hunan, PR China. Electronic address:

The present study was designed to explore the role of transient receptor potential canonical 3 (TRPC3) in nicotine-induced chronic obstructive pulmonary disease (COPD) and its underlying mechanism. In this study, the expression and localization of α5 nicotinic acetylcholine receptor (α5-nAchR) in lung tissues were determined by western blotting and immunohistochemistry. The quantitative real-time PCR (qRT-PCR) analysis was performed to examine the mRNA expression levels of α5-nAchR and TRPC3 in human airway smooth muscle cells (HASMCs). Read More

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December 2018

Inflammation and oxidative stress are key mediators in AKB48-induced neurotoxicity in vitro.

Toxicol In Vitro 2018 Dec 11. Epub 2018 Dec 11.

Istanbul University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Beyazıt, 34116 Istanbul, Turkey.

Synthetic cannabinoids were introduced into market in early 2000s; since these "legal highs" are dramatically popular among youth, it becomes a deadly problem. Synthetic cannabinoids have high affinity to cannabinoid receptors; leading to various clinical symptoms. AKB48 (Apinaca) has been classified as a third-generation synthetic cannabinoid for the first time in 2014. Read More

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December 2018
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