18,089,412 results match your criteria Human Parasitic Diseases [Journal]

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Translating the application of TGF-β1, chondroitinase-ABC, and lysyl oxidase-like 2 for mechanically robust tissue-engineered human neocartilage.

J Tissue Eng Regen Med 2018 Dec 17. Epub 2018 Dec 17.

Department of Biomedical Engineering, University of California Irvine, Irvine, CA, USA.

Strategies to overcome the limited availability of human articular chondrocytes (hACs) and their tendency to dedifferentiate during expansion are required to advance their clinical use and to engineer functional cartilage on par with native articular cartilage. This work sought to determine whether a biochemical factor, TGF-β1 (T), a biophysical agent, chondroitinase-ABC (C), and a collagen crosslinking enzyme, lysyl oxidase-like 2 (L) are efficacious in forming three-dimensional human neocartilage from expanded human articular chondrocytes. Among the treatment regimens, the combination of the three stimuli (TCL treatment) led to the most robust glycosaminoglycan (GAG) content, total collagen content, and type II collagen production. Read More

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December 2018

Supplementation of Modified Mannitol-Yolk-Polymyxin B Agar with Cefuroxime for Quantitative Detection of Bacillus cereus in Food.

J Food Sci 2018 Dec 17. Epub 2018 Dec 17.

the Konkuk Univ., Hwayang-dong, Gwangjin-gu, Seoul, The Republic of Korea.

The presence of unwanted competing flora has been the most common confounding factor in the enumeration of Bacillus cereus (B. cereus) using selective media such as mannitol-yolk-polymyxin B agar (MYPA). The objective of this study was to improve MYPA selectivity for B. Read More

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December 2018

Nodules and masses are associated with malignant pleural effusion in dogs and cats but many other intrathoracic CT features are poor predictors of the effusion type.

Vet Radiol Ultrasound 2018 Dec 17. Epub 2018 Dec 17.

Department of Clinical Studies New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square, PA, 19348.

Thoracic CT may be used in the workup of patients with pleural effusion. In humans, certain pleural features on CT aid in diagnosing an underlying cause for pleural effusion, whereas this is not well studied in veterinary medicine. This retrospective cross-sectional analytical study assessed pleural and other intrathoracic abnormalities on CT in dogs and cats with pleural effusion and explored potential discriminatory features between effusion types. Read More

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December 2018

Assessment of Exercise Capacity in Children with Type 1 Diabetes in the Cooper Running Test.

Int J Sports Med 2018 Dec 17. Epub 2018 Dec 17.

Department of Pediatrics, Diabetology, Endocrinology and Nephrology, Medical University of Lodz, Lodz, Poland.

Regular physical activity increases lifespan for those with type 1 diabetes. However, disease-related barriers may deter children from exercise and affect their fitness. This study examined the safety of the Cooper test concerning diabetes-related acute complications in children with type 1 diabetes and their fitness. Read More

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December 2018

Anthropometry, Physical and Movement Features, and Repeated-sprint Ability in Soccer Players.

Int J Sports Med 2018 Dec 17. Epub 2018 Dec 17.

Department of Biomedical and Neuromotor Science, University of Bologna, Bologna, Italy.

This study aimed to evaluate the associations of anthropometry, functional movement patterns (FMP) and physical performance characteristics with repeated-sprint ability (RSA) in male youth soccer players. Thirty six athletes (ages 16.6±0. Read More

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December 2018

Effect of Different Circuit Training on Cardiovascular Responses in Cirrhotic Patients.

Int J Sports Med 2018 Dec 17. Epub 2018 Dec 17.

Universidade Federal do Rio de Janeiro, School of Physical Education and Sports, Rio de Janeiro, Brazil.

The purpose of this study was to analyze and compare heart rate (HR), heart rate variability (HRV), and blood pressure (BP) responses during and after circuit resistance training with 1 min. (CIR1) vs. 2 min. Read More

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December 2018

Platelet-Dense Granules Worsen Pre-Infection Thrombocytopenia during Gram-Negative Pneumonia-Derived Sepsis.

J Innate Immun 2018 Dec 17:1-13. Epub 2018 Dec 17.

Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.

Platelet-dense (δ) granules are important for platelet function. Platelets contribute to host defense and vascular integrity during pneumonia and sepsis, and δ granule products, including adenosine diphosphate (ADP), can influence inflammatory responses. We therefore aimed to study the role of platelet δ granules in the host response during sepsis. Read More

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December 2018

Cubilin Single Nucleotide Polymorphism Variants are Associated with Macroangiopathy While a Matrix Metalloproteinase-9 Single Nucleotide Polymorphism Flip-Flop may Indicate Susceptibility of Diabetic Nephropathy in Type-2 Diabetic Patients.

Nephron 2018 Dec 17:1-10. Epub 2018 Dec 17.

Clinic of Nephrology and Hypertension, Diabetes and Endocrinology, Otto-von-Guericke University, Magdeburg, Germany.

Aim: Aim of this study was to investigate the association of genetic variants of functional polymorphisms of matrix metalloproteinase and Cubilin (CUBN) with diabetic nephropathy (DN), end-stage renal disease (ESRD), and risk of cardiovascular disease (CVD) in Caucasian type 2 diabetes (T2D) patients.

Methods: 472 T2D-patients were genotyped for 3 single-nucleotide polymorphisms (SNPs; MMP-2 [rs2285053], MMP-9 [rs17576] and CUBN [rs1801239]). Genotyping was carried out by allelic discrimination using TaqMan SNP-genotyping-assay. Read More

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December 2018

The Role of Toll-Like Receptors in the Production of Cytokines by Human Lung Macrophages.

J Innate Immun 2018 Dec 17:1-11. Epub 2018 Dec 17.

Département des Maladies Respiratoires, Hôpital Foch, Suresnes, France.

Background: The Toll-like receptor (TLR) family is involved in the recognition of and response to microbial infections. These receptors are expressed in leukocytes. TLR stimulation induces the production of proinflammatory cytokines and chemokines. Read More

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December 2018

Primary Human Neutrophils Exhibit a Unique HIV-Directed Antibody-Dependent Phagocytosis Profile.

J Innate Immun 2018 Dec 17:1-10. Epub 2018 Dec 17.

The only clinical HIV vaccine trial to demonstrate efficacy, RV144, correlated protection with the antibodies (Abs) mediating function via the "constant" immunoglobulin region, the crystallizable fragment (Fc). These data have supported a focus on the induction of Abs by vaccines that trigger antiviral activities by relevant leukocytes via Fc receptors (FcRs). Neutrophils are phagocytes that comprise > 50% of leukocytes and display unique FcRs. Read More

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December 2018
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Towards Understanding the Molecular Basis of Nitric Oxide-Regulated Group Behaviors in Pathogenic Bacteria.

J Innate Immun 2018 Dec 17:1-11. Epub 2018 Dec 17.

Pathogenic bacteria have many strategies for causing disease in humans. One such strategy is the ability to live both as single-celled motile organisms or as part of a community of bacteria called a biofilm. Biofilms are frequently adhered to biotic or abiotic surfaces and are extremely antibiotic resistant. Read More

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December 2018

Downregulation of Microparticle Release and Pro-Inflammatory Properties of Activated Human Polymorphonuclear Neutrophils by LMW Fucoidan.

J Innate Immun 2018 Dec 17:1-17. Epub 2018 Dec 17.

Laboratório de Farmacologia Celular e Molecular, Universidade do Estado do Rio de Janeiro, Rio de Janeiro,

Exposition of neutrophils (polymorphonuclear neutrophils, PMNs) to bacterial products triggers exacerbated activation of these cells, increasing their harmful effects on host tissues. We evaluated the possibility of interfering with the classic immune innate responses of human PMNs exposed to bacterial endotoxin (lipopolysaccharide, LPS), and further stimulated with bacterial formyl peptide (N-formyl-methionine-leucine-phenylalanine, fMLP). We showed that the low- molecular-weight fucoidan (LMW-Fuc), a polysaccharide extracted from brown algae, attenuated the exacerbated activation induced by fMLP on LPS-primed PMNs, in vitro, impairing chemotaxis, NET formation, and the pro-survival and pro-oxidative effects. Read More

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December 2018

Fluid distribution and cell integrity indicators evaluated by bioelectrical impedance in university athletes: comparison between team sports and individual sports.

Physiol Meas 2018 Dec 17. Epub 2018 Dec 17.

Research Center in Kinanthropometry and Human Performance, Federal University of Santa Catarina, Florianopolis, BRAZIL.

The objective was to compare indicators of fluid distribution and cellular integrity in university athletes. Cross-sectional study with 167 university athletes (18-35 years), of team and individual sports. The bioimpedance was used to measure body water (TBW), intracellular water (ICW), extracellular water (ECW), ECW/ICW, impedance (Z), reactance (Xc), resistance (R), phase angle, body cell mass (BCM) and ECW/BCM at frequency 50khz. Read More

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December 2018

Controllable fabrication of hydroxybutyl chitosan/oxidized chondroitin sulfate hydrogels by 3D bioprinting technique for cartilage tissue engineering.

Biomed Mater 2018 Dec 17. Epub 2018 Dec 17.

Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital Affiliated Shanghai Jiao Tong University School of Medicine, Shanghai, CHINA.

Biological regeneration of articular cartilage continues to be a challenge at present. Functional engineered implants with patient-specific sizes are difficult to achieve. The aim of this study is to fabricate a biocompatible cell-laden hydrogel with a designable structure. Read More

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December 2018

A smart nanosensor for the detection of human immunodeficiency virus and associated cardiovascular and arthritis diseases using functionalized graphene-based transistors.

Biosens Bioelectron 2018 Dec 7;126:792-799. Epub 2018 Dec 7.

DBT-National Institute of Animal Biotechnology (DBT-NIAB), Hyderabad 500032, Telangana, India. Electronic address:

Human immunodeficiency virus (HIV), which isa worldwide public health issue, is commonly associated with cardiovascular disorders (CVDs) and rheumatoid arthritis (RA). A smart nanosensor was developed for the detection of HIV and its related diseases (CVDs and RA) using graphene-based field-effect transistors (FETs). In this study, amine-functionalized graphene (afG) was conjugated with antibodies [anti-p24 for HIV, anti-cardiac troponin 1 (anti-cTn1) for CVDs, and anti-cyclic citrullinated peptide (anti-CCP) for RA] to detect various biomarkers. Read More

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December 2018

Identification of novel PI3Kδ inhibitors by docking, ADMET prediction and molecular dynamics simulations.

Comput Biol Chem 2018 Dec 7;78:190-204. Epub 2018 Dec 7.

Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, China. Electronic address:

Background: Phosphoinositide-3-kinase Delta (PI3Kδ) plays a key role in B-cell signal transduction and inhibition of PI3Kδ is confirmed to have clinical benefit in certain types of activation of B-cell malignancies. Virtual screening techniques have been used to discover new molecules for developing novel PI3Kδ inhibitors with little side effects.

Method: Computer aided drug design method were used to rapidly screen optimal PI3Kδ inhibitors from the Asinex database. Read More

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December 2018

Association between exposure to a mixture of phenols, pesticides, and phthalates and obesity: Comparison of three statistical models.

Environ Int 2018 Dec 14;123:325-336. Epub 2018 Dec 14.

State Key Laboratory of Reproductive Medicine, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China. Electronic address:

Background: The evaluation of the chemical impact on human health is usually constrained to the analysis of the health effects of exposure to a single chemical or a group of similar chemicals at one time. The effects of chemical mixtures are seldom analyzed. In this study, we applied three statistical models to assess the association between the exposure to a mixture of seven xenobiotics (three phthalate metabolites, two phenols, and two pesticides) and obesity. Read More

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December 2018

Therapeutic blockade of the interleukin-6 receptor (IL-6R) allows sIL-6R generation by proteolytic cleavage.

Cytokine 2018 Dec 14;114:1-5. Epub 2018 Dec 14.

Department of Pathology, Otto-von-Guericke-University Magdeburg, Medical Faculty, Magdeburg, Germany. Electronic address:

Blockade of the interleukin-6 receptor (IL-6R) is a successful therapeutic strategy in various inflammatory diseases. IL-6 can signal via membrane-bound (classic signaling) and soluble forms (sIL-6R, trans-signaling) of the IL-6R. Trans-signaling is causative for the pro-inflammatory properties of IL-6, and the selective inhibition of this pathway holds the promise to cause less side effects than the global blockade of IL-6 signaling. Read More

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December 2018

Parabens and triclosan in shellfish from Shenzhen coastal waters: Bioindication of pollution and human health risks.

Environ Pollut 2018 Dec 7;246:257-263. Epub 2018 Dec 7.

Institute of Environmental Pollution and Health, School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, PR China; Guangzhou Key Laboratory of Environmental Catalysis and Pollution Control, School of Environmental Science and Engineering, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou 510006, Guangdong, PR China. Electronic address:

This work aimed to determine the concentrations of parabens and triclosan (TCS) in shellfish from coastal waters of Shenzhen, South China. A method of isotope dilution with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was used to determine TCS and five paraben analogues, including methyl paraben (MeP), ethyl paraben (EtP), propyl paraben (PrP), butyl paraben (BuP), and benzyl paraben (BeP), in 186 shellfish samples covering eight species. Concentrations of parabens and TCS were 0. Read More

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December 2018

The facile synthesis of copper oxide quantum dots on chitosan with assistance of phyto-angelica for enhancing the human osteoblast activity to the application of osteoporosis.

J Photochem Photobiol B 2018 Nov 14;191:6-12. Epub 2018 Nov 14.

Department of Orthopedics, Xiaoshan Traditional Chinese Medical Hospital, 156 Yucai Road, Hangzhou, Zhejiang 311201, PR China.

Osteoblasts are an important key factor for the pathogenesis of several bone-related diseases, notably in osteoporosis. Osteoporosis is a disorder categorized based on the bone mineral density (BMD) and an alteration in the bone micro-architecture had been considered as the major determinant for increasing the fracture risk. The available medicine for curing the osteoporosis shows a minimal or no activity against the genesis or function of osteoblasts. Read More

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November 2018

Biosynthesized of reduced graphene oxide nanosheets and its loading with paclitaxel for their anti cancer effect for treatment of lung cancer.

J Photochem Photobiol B 2018 Nov 22;191:13-17. Epub 2018 Nov 22.

Department of pharmacy, Fujian Provincial Hospital& Fujian Medical University, People's Republic of China; Molecular Biology Laboratory of Chinese Traditional Medicine, Fujian Provincial Hospital, People's Republic of China. Electronic address:

Multifunctional nanocarriers are in immediate need to develop anticancer activity for the treatment of cancers. In the present research, the graphene oxide was reduced via an efficient method which reduced to RGO by using Euphorbia milii leaves extract. Thus obtained RGO nanocomposites were subsequently characterized by means UV-Vis absorption technique. Read More

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November 2018

Social housing protects against age-related working memory decline independently of physical enrichment in rats.

Neurobiol Aging 2018 Nov 23;75:117-125. Epub 2018 Nov 23.

Psychology Department, Providence College, Providence, RI, USA.

Longitudinal human studies suggest that as we age, sociality provides protective benefits against cognitive decline. However, little is known about the underlying neural mechanisms. Rodent studies, which are ideal for studying cognition, fail to examine the independent effects of social housing while controlling for physical enrichment in all groups. Read More

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November 2018

Schizophrenia patients are impaired in recognition task but more for intentionality than physical causality.

Conscious Cogn 2018 Dec 14;67:98-107. Epub 2018 Dec 14.

Laboratoire HANDIReSP (EA4047), Université de Versailles Saint-Quentin-En-Yvelines, Versailles, France; Service de Psychiatrie de l'Adulte et d'Addictologie, Centre Hospitalier de Versailles, Le Chesnay, France.

It is now largely accepted that patients with schizophrenia have a deficit to attribute mental states to others, such as intentions, needs and motivations but also to perform memory tasks. According to one hypothesis, these impairments may be due to an early visual attention deficit during the encounter of social stimuli. Another hypothesis posits a robust correlation between intention attribution and autobiographical memory that results in impaired recollection of past events making it difficult to infer others' intentions. Read More

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December 2018

Current perspectives of Australian therapists on rehabilitation and return to sport after anterior cruciate ligament reconstruction: A survey.

Phys Ther Sport 2018 Dec 10;35:139-145. Epub 2018 Dec 10.

Perth Orthopaedic and Sports Medicine Centre, West Perth, Western Australia, Australia.

Objectives: To investigate views and practices of Australian therapists on rehabilitation and return to sport (RTS) after anterior cruciate ligament reconstruction (ACLR).

Design: Survey-based study.

Setting: Online survey platform. Read More

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December 2018

Chronic stress exposure and daily stress appraisals relate to biological aging marker p16.

Psychoneuroendocrinology 2018 Dec 7;102:139-148. Epub 2018 Dec 7.

Cousins Center for Psychoneuroimmunology, Semel Institute for Neuroscience and Human Behavior, University of California, 300 Medical Plaza, Los Angeles, CA 90095, USA; Department of Psychology, University of California, 502 Portola Plaza, Los Angeles, CA 90095, USA. Electronic address:

Previous research has linked exposure to adverse social conditions with DNA damage and accelerated telomere shortening, raising the possibility that chronic stress may impact biological aging pathways, ultimately increasing risk for age-related diseases. Less clear, however, is whether these stress-related effects extend to additional hallmarks of biological aging, including cellular senescence, a stable state of cell cycle arrest. The present study aimed to investigate associations between psychosocial stress and two markers of cellular aging-leukocyte telomere length (LTL) and cellular senescence signal p16. Read More

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December 2018

Gaze allocation in face-to-face communication is affected primarily by task structure and social context, not stimulus-driven factors.

Cognition 2018 Dec 14;184:28-43. Epub 2018 Dec 14.

Experimental Psychology, Helmholtz Institute, Utrecht University, Utrecht, the Netherlands; Developmental Psychology, Utrecht University, Utrecht, the Netherlands; Brain Center Rudolf Magnus, University Medical Center, Utrecht, the Netherlands.

Gaze allocation to human faces has recently been shown to be greatly dependent on the social context. However, what has not been considered explicitly here, is how gaze allocation may be supportive of the specific task that individuals carry out. In the present study, we combined these two insights. Read More

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December 2018

Prevalence of Germline Mutations in Polyposis and Colorectal Cancer-associated Genes in Patients With Multiple Colorectal Polyps.

Clin Gastroenterol Hepatol 2018 Dec 14. Epub 2018 Dec 14.

Ambry Genetics, Aliso Viejo, CA.

Background And Aims: Guidelines recommend genetic testing of patients with 10 or more cumulative adenomatous polyps. However, little is known about the utility of these tests-especially for older patients. We aimed to determine the prevalence of pathogenic mutations in patients with multiple colorectal polyps, stratified by age. Read More

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December 2018

Maternal urinary benzophenones and infant birth size: Identifying critical windows of exposure.

Chemosphere 2018 Nov 28;219:655-661. Epub 2018 Nov 28.

Key Laboratory of Environment and Health, Ministry of Education & Ministry of Protection, and State Key Laboratory of Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. Electronic address:

Benzophenones (BPs) are widely used as ultraviolet absorbers and fragrance retention agents. Evidences from animal studies have suggested that exposure to BPs may affect fetal growth, but human data is limited and no study is concerning critical windows of BPs exposure throughout pregnancy in relation to fetal growth. We aimed to investigate the associations of prenatal exposure to BPs with birth size and examine the critical exposure windows of fetus development. Read More

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November 2018

Mechanism and kinetics studies of the atmospheric oxidation of p,p'-Dicofol by OH and NO radicals.

Chemosphere 2018 Dec 10;219:645-654. Epub 2018 Dec 10.

Environment Research Institute, Shandong University, Jinan, 250100, China.

As an effective organochlorine pesticide, Dicofol has been extensively applied in more than 30 countries for protecting over 60 different crops. Considering its large consumption and potential adverse effect on human health (endocrine disrupting and carcinogenicity), the fate of Dicofol sprayed into the air is of public concern. In this study, we conducted a comprehensive study on the reaction mechanisms of p,p'-Dicofol with OH and NO radicals using DFT method. Read More

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December 2018

Metabolic perturbation, proliferation and reactive oxygen species jointly contribute to cytotoxicity of human breast cancer cell induced by tetrabromo and tetrachloro bisphenol A.

Ecotoxicol Environ Saf 2018 Dec 14;170:495-501. Epub 2018 Dec 14.

State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, Hong Kong, China. Electronic address:

Halogenated bisphenol A analogues (X-BPA) have been widely used in industrial production, such as flame retardant. Although BPA exposure was found to result in cytotoxicity, toxicity of X-BPA and molecular mechanism remain under-explored. In this study, we employed human breast cancer cell as a model to investigate the concentration-dependent toxicity and underlying mechanisms of tetrabromo bisphenol A (TBBPA) and tetrachloro bisphenol A (TCBPA). Read More

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December 2018

The Intestinal Lymphatic System: Functions and Metabolic Implications.

Cell Mol Gastroenterol Hepatol 2018 Dec 14. Epub 2018 Dec 14.

Cardiovascular Research Center and Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06510-3221, USA; INSERM U970, Paris Cardiovascular Research Center, 75015 Paris, France.

The lymphatic system of the gut plays important roles in the transport of dietary lipids, as well as in immunosurveillance and removal of interstitial fluid. Historically, despite its crucial functions in intestinal homeostasis, the lymphatic system has been poorly studied. In the last two decades, identification of specific molecular mediators of lymphatic endothelial cells (LECs) growth together with novel genetic approaches and intravital imaging techniques, have advanced our understanding of the mechanisms regulating intestinal lymphatic physiology in health and disease. Read More

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December 2018

A mouse model for intellectual disability caused by mutations in the X-linked 2'‑O‑methyltransferase Ftsj1 gene.

Biochim Biophys Acta Mol Basis Dis 2018 Dec 14. Epub 2018 Dec 14.

Department of Functional Genomics, Interfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, Felix-Hausdorff-Strasse 8, 17489 Greifswald, Germany.

Mutations in the X chromosomal tRNA 2'‑O‑methyltransferase FTSJ1 cause intellectual disability (ID). Although the gene is ubiquitously expressed affected individuals present no consistent clinical features beyond ID. In order to study the pathological mechanism involved in the aetiology of FTSJ1 deficiency-related cognitive impairment, we generated and characterized an Ftsj1 deficient mouse line based on the gene trapped stem cell line RRD143. Read More

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December 2018

Multiple integrin ligands provide a highly adhesive and osteoinductive surface that improves selective cell retention technology.

Acta Biomater 2018 Dec 14. Epub 2018 Dec 14.

National & Regional United Engineering Lab of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China; Center of Regenerative and Reconstructive Engineering Technology in Chongqing City, Chongqing, China. Electronic address:

Among various bone tissue engineering strategies, selective cell retention (SCR) technology has been used as a practical clinical method for bone graft manufacturing in real time. The more mesenchymal stem cells (MSCs) are retained, the better the osteoinductive microenvironment provided by the scaffold, which in turn promotes the osteogenesis of the SCR-fabricated bone grafts. Integrin receptors are crucial to cell-matrix adhesion and signal transduction. Read More

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December 2018

Assessing the in vivo data on low/no-calorie sweeteners and the gut microbiota.

Food Chem Toxicol 2018 Dec 14. Epub 2018 Dec 14.

University of Reading, Department of Food and Nutritional Sciences, PO Box 226, Whiteknights, Reading, RG6 6AP, UK.

Low/no-calorie sweeteners (LNCS) are continually under the spotlight in terms of their safety and benefits; in 2014 a study was published linking LNCS to an enhanced risk of glucose intolerance through modulation of the gut microbiota. In response, an in-depth review of the literature was undertaken to evaluate the major contributors to potential changes in the gut microbiota and their corresponding sequelae, and to determine if consuming LNCS (e.g. Read More

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December 2018

Identification of the proteoforms of surface localized Rod A of Aspergillus flavus and determination of the mechanism of proteoform generation.

J Proteomics 2018 Dec 14. Epub 2018 Dec 14.

Department of Proteomics, Aravind Medical Research Foundation, Aravind Eye Care System, Dr. G. Venkataswamy Eye Research Institute, No:1 Anna Nagar, Madurai, TN, India. Electronic address:

Fungal keratitis is a serious, potentially sight-threatening corneal infection that is more prevalent in the tropical parts of the world including India, and A. flavus and Fusarium solani are the predominant etiological agents. The surface of fungal conidia is covered by hydrophobin family proteins, effectively masking the conidial antigens from immune cells. Read More

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December 2018

A multiomic approach to characterize the temporal sequence in Alzheimer's disease-related pathology.

Neurobiol Dis 2018 Dec 14. Epub 2018 Dec 14.

Institute of Biomedicine, University of Eastern Finland, Kuopio 70210, Finland. Electronic address:

No single-omic approach completely elucidates the multitude of alterations taking place in Alzheimer's disease (AD). Here, we coupled transcriptomic and phosphoproteomic approaches to determine the temporal sequence of changes in mRNA, protein, and phosphopeptide expression levels from human temporal cortical samples, with varying degree of AD-related pathology. This approach highlighted fluctuation in synaptic and mitochondrial function as the earliest pathological events in brain samples with AD-related pathology. Read More

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December 2018

MALDI-TOF MS for rapid diagnosis of Anaerobiospirillum succiniciproducens, an unusual causative agent of bacteraemia in humans. Two case reports and literature review.

Anaerobe 2018 Dec 14. Epub 2018 Dec 14.

Clinical Microbiology Department, Hospital Universitario La Paz, Madrid, Spain.

Anaerobiospirillum succiniciproducens is a gram-negative anaerobic spiral rod which is part of the normal flora of dogs and cats and can produce bacteraemia and diarrhoea in humans. In this report we describe two cases of bacteraemia caused by A. succiniciproducens which was successfully identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Read More

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December 2018

The Retrotransverse foramen of the atlas is not a modern anatomic variation.

World Neurosurg 2018 Dec 14. Epub 2018 Dec 14.

Department of Human Anatomy and Physiology, Faculty of Health Sciences, University of Johannesburg, Auckland Park, 2006, South Africa.

Background: The retrotransverse foramen (RTF) is a nonmetric variant of the atlas vertebra that consists of an abnormal accessory foramen located on the posterior root of the transverse process and it extends from the posterior root of the transverse process to the root of the posterior arch. Its presence has been related to regional variations of the venous circulation. It is currently unknown whether the RTF is a modern or an ancient anatomic variation. Read More

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December 2018

5-methylcytosine and 5-hydroxymethylcytosine in brains of patients with multiple system atrophy and patients with Parkinson's disease.

J Chem Neuroanat 2018 Dec 14. Epub 2018 Dec 14.

Department of Neurology, University of Bonn, Germany; German Center for Neurodegenerative Diseases (DZNE), University of Bonn, Germany.

Multiple system atrophy (MSA) is a sporadic neurodegenerative disorder of unknown etiology, characterized pathologically by α-synuclein aggregates preferentially found in oligodendroglial cells. DNA methylation has emerged as a mechanism of regulation of α-synuclein expression. Reduced 5-methylcytosine (5-mC) DNA methylation of α-synuclein has been found in the brains of patients with Parkinson's disease (PD). Read More

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December 2018

Extracellular vesicles enhance the targeted delivery of immunogenic oncolytic adenovirus and paclitaxel in immunocompetent mice.

J Control Release 2018 Dec 14. Epub 2018 Dec 14.

Department of Oncology and Hemato-Oncology, Center of Excellence on Neurodegenerative Diseases, University of Milan, Milan, Italy. Electronic address:

Extracellular vesicles (EVs), are naturally occurring cargo delivery tools with the potential to be used as drug vehicles of single agents or combination therapies. We previously demonstrated that human lung cancer cell-derived EVs could be used for the systemic delivery of oncolytic virus (OVs) and chemotherapy drugs such as paclitaxel (PTX), leading to enhanced anti-tumor effects in nude mice. In the current work, we evaluated the biodistribution of EVs by using bioluminescence and fluorescence imaging technologies, thus proving the ability of these EVs-formulations to specifically target the neoplasia, while leaving other body tissues unaffected. Read More

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December 2018

Enhanced intranasal insulin delivery by formulations and tumor protein-derived protein transduction domain as an absorption enhancer.

J Control Release 2018 Dec 14. Epub 2018 Dec 14.

Department of Pharmaceutics and Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.

One of the key factors for successful development of an intranasal insulin formulation is an absorption enhancer that would deliver insulin efficiently across nasal membranes without causing damage to mucosa or inducing protein aggregation under physiological conditions. In the present study, a protein transduction domain (PTD1) and its L-form with the double substitution A6L and I8A (PTD4), derived from human translationally controlled tumor protein, were used as absorption enhancers. PTD4 exhibited higher compatibility with insulin in terms of biophysical properties analyzed using μDSC, DLS, and CD. Read More

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December 2018

Detection of high-risk human papillomavirus DNA in tissue from primary cervical cancer tumor, pelvic lymph nodes and recurrent disease.

Papillomavirus Res 2018 Dec 14. Epub 2018 Dec 14.

Department of Pathology, Aarhus University Hospital, Aarhus, Denmark.

Objectives: The present study investigated Human Papillomavirus (HPV) DNA genotyping in primary tumor, pelvic lymph nodes (PLN) and recurrence in early-stage cervical cancer patients.

Methods: We conducted a hospital-based case-control study. From 2003 to 2015, 282 patients underwent surgery for cervical cancer in the Department of Gynecology, Aarhus University Hospital, Denmark. Read More

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December 2018

Organic Anion Transporting Polypeptide 2B1 - More than a Glass-Full of Drug Interactions.

Pharmacol Ther 2018 Dec 14. Epub 2018 Dec 14.

University of Washington, School of Pharmacy, Department of Pharmaceutics, Seattle, WA, USA. Electronic address:

The importance of uptake transporters in determining drug disposition is increasingly appreciated. While the focus of regulatory agencies worldwide has been on the hepatic organic anion transporting polypeptides (OATPs) 1B1 and 1B3, there is another isoform of the OATP sub-family, OATP2B1, which should be considered equally relevant. Unlike the other members of the OATP sub-family, OATP2B1 is expressed in multiple organs in humans, including in the intestine and the liver. Read More

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December 2018

Diurnal Regulation of Sphingolipids in Blood.

Biochim Biophys Acta Mol Cell Biol Lipids 2018 Dec 14. Epub 2018 Dec 14.

Department of General Pharmacology and Toxicology, Goethe University Hospital, Frankfurt am Main, Germany.

Key homeostatic functions are regulated in a diurnal manner and a miss-alignment of such rhythms is believed to contribute to the pathophysiology of several diseases. Signaling sphingolipids (SLs) in plasma such as sphingosine 1-phosphate control lymphocytic trafficking, vascular reactivity and platelet activity, physiological functions all of which display a diurnal rhythm themselves. However, the rhythmicity of SL metabolism in plasma and its potential causes have not been sufficiently investigated so far. Read More

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December 2018

Single-molecule 3D imaging of human plasma intermediate-density lipoproteins reveals a polyhedral structure.

Biochim Biophys Acta Mol Cell Biol Lipids 2018 Dec 14. Epub 2018 Dec 14.

The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Electronic address:

Intermediate-density lipoproteins (IDLs), the remnants of very-low-density lipoproteins via lipolysis, are rich in cholesteryl ester and are correlated with cardiovascular disease. Despite pharmacological interests in IDL, the three-dimensional (3D) structure of IDLs is still undetermined due to their variation in size, compositions, and dynamic structure. To explore the 3D structure of IDL, we reconstructed 3D density maps from each individual IDL particles using cryo-electron microscopy (cryo-EM) and individual-particle electron tomography (IPET, without averaging from different molecules). Read More

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December 2018

Induced Pluripotent Stem Cells: A New Strategy to Model Human Cancer.

Int J Biochem Cell Biol 2018 Dec 14. Epub 2018 Dec 14.

Laboratory for Cancer Biology, Department of Medical Oncology and Clinical Research, Cancer Institute (WIA), Chennai, India. Electronic address:

Induced pluripotent stem cells are derived from adult somatic cells by ectopic expression of stem cell factors OCT4, SOX2, MYC and KLF4. These cells have characteristic features similar to embryonic stem cells. Although there exists in vitro and in vivo models of cancer, recapitulating the earliest events in the pathogenesis remain challenging. Read More

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December 2018

Engineering Two Species of Yeast as Cell Factories for 2'-Fucosyllactose.

Metab Eng 2018 Dec 14. Epub 2018 Dec 14.

E.I. du Pont de Nemours and Company (DuPont), Industrial Biosciences, Experimental Station, Wilmington, Delaware 19803; Central Research and Development, Industrial Biosciences, Experimental Station, Wilmington, Delaware 19803.

Oligosaccharides present in human breast milk have been linked to beneficial effects on infant health. Inclusion of these human milk oligosaccharides (HMOs) in infant formula can recapitulate these health benefits. As a result, there is substantial commercial interest in a cost-effective source of HMOs as infant formula ingredients. Read More

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December 2018

The effect of dietary carotenoid increase on painted turtle spot and stripe color.

Comp Biochem Physiol B Biochem Mol Biol 2018 Dec 14. Epub 2018 Dec 14.

Department of Biology, PO Box 5000, Shepherd University, Shepherdstown, WV. USA. 25443.

Determination of how carotenoid-based color patches change with pigmentary access is important to understanding color patch function in animals. We performed a carotenoid addition experiment on male painted turtles (Chrysemys picta) to determine if an increase in dietary carotenoids led to location-specific changes in painted turtle spot and stripe color. Turtles fed large quantities of carotenoids had increased yellow chroma in chin stripes and increased red chroma in the neck and carapace stripes, as well as reduced ultraviolet (UV) chroma in the neck (orange to the human eye) and fore -limb (red to the human eye) stripes. Read More

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December 2018

A tale of two steroids: the importance of the androgens DHEA and DHEAS for early neurodevelopment.

J Steroid Biochem Mol Biol 2018 Dec 14. Epub 2018 Dec 14.

School of Health & Biomedical Sciences, RMIT University - Bundoora Campus, Melbourne, 3083, Australia. Electronic address:

DHEA and DHEAS are neuroactive neurosteroids that interact with several major receptor system in the brain, including sigma (σ), glutamate, and GABA-A receptors. It has been recognized as early as 1952, that the loss of DHEA/DHEAS in adult life is associated with neuropsychiatric disorders (eg schizophrenia, depression). However, the mechanistic role for DHEA/DHEAS in any of these domains remains speculative, not the least because the presence of these androgens in the adrenal gland and brain is largely confined to humans and only some non-human primates. Read More

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December 2018

TCF7L2 and EGR1 synergistic activation of transcription of LCN2 via an ERK1/2-dependent pathway in esophageal squamous cell carcinoma cells.

Cell Signal 2018 Dec 14. Epub 2018 Dec 14.

Department of Pathology, Shantou Central Hospital, Affiliated Shantou Hospital of Sun Yat-sen University, Shantou 515041, China; Guangdong Province Key Laboratory of Malignant Tumor Epigenetics and Genes Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China. Electronic address:

High level expression of lipocalin 2 (LCN2) usually indicates poor prognosis in esophageal squamous cell carcinoma (ESCC) and many other cancers. Our previous study showed LCN2 promotes migration and invasion of ESCC cells through a novel positive feedback loop. However, the key transcription activation protein (KTAP) in the loop had not yet been identified. Read More

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December 2018
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