5,108 results match your criteria Human Immunology [Journal]


B cells as organizers of immunity in transplantation.

Authors:
Marilia Cascalho

Hum Immunol 2019 Apr 20. Epub 2019 Apr 20.

Transplantation Biology, University of Michigan, Ann Arbor, MI, United States.

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http://dx.doi.org/10.1016/j.humimm.2019.04.010DOI Listing

Proactive treatment of angiotensin receptor antibodies in kidney transplantation with plasma exchange and/or candesartan is safe and associated with excellent graft survival at 4 years: A single centre Australian experience.

Hum Immunol 2019 Apr 20. Epub 2019 Apr 20.

South Australian Tissue Typing and Immunogenetics Service, Australian Red Cross Blood Service (ARCBS), Adelaide, South Australia, Australia; Central Northern Adelaide Renal and Transplantation Services (CNARTS), Royal Adelaide Hospital, Adelaide, South Australia, Australia.

High levels of angiotensin receptor antibodies (ATRab) are associated with acute cellular and humoral rejection, vascular occlusion, de novo human leucocyte antigen donor specific antibody (HLA DSA) and poor graft survival in kidney transplant recipients (KTR). Since 2015 we proactively managed patients "at risk" (AR) with ATRab >17 U/ml with perioperative plasma exchange (PLEX) and/or angiotensin receptor blockade (ARB). 44 patients were treated with this protocol. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.04.005DOI Listing

Antibodies against Angiotensin II Type 1 and Endothelin A Receptors: Relevance and pathogenicity.

Hum Immunol 2019 Apr 19. Epub 2019 Apr 19.

Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, United States.

Antibodies against two G-protein coupled receptors (GPCRs), angiotensin II type 1 receptor (AT1R) and endothelin A receptor (ETAR) are among a growing number of autoantibodies that are found to be associated with allograft dysfunction. AT1R antibodies (AT1Rabs) and ETAR antibodies (ETARabs) have been shown to activate their target receptors and affect signaling pathways. Multiple single center reports have shown an association between presence of these antibodies and acute or chronic rejection and graft loss in kidney, heart, liver, lung and composite tissue transplantations. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01988859193013
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http://dx.doi.org/10.1016/j.humimm.2019.04.012DOI Listing
April 2019
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The impact of non-HLA antibodies on outcomes after lung transplantation and implications for therapeutic approaches.

Authors:
Ramsey R Hachem

Hum Immunol 2019 Apr 17. Epub 2019 Apr 17.

Washington University School of Medicine, Division of Pulmonary & Critical Care, 4523 Clayton Ave., Campus Box 8052, St. Louis, MO 63110, United States. Electronic address:

The role of donor-specific antibodies (DSA) to mismatched human leukocyte antigens (HLA) in lung allograft rejection has been recognized over the past 20 years. During this time, there has been growing experience and recognition of an important role for non-HLA antibodies in lung allograft rejection. Multiple self-antigens have been identified that elicit autoimmune responses including collagen V, K-α 1 tubulin, angiotensin type 1 receptor, and endothelin type A receptor, but it is likely that other antigens elicit similar responses. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01988859193014
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http://dx.doi.org/10.1016/j.humimm.2019.04.008DOI Listing
April 2019
1 Read

Angiotensin II type I receptor antibodies in thoracic transplantation.

Hum Immunol 2019 Apr 10. Epub 2019 Apr 10.

HLA and Immunogenetics Laboratory, Comprehensive Transplant Center, Cedars-Sinai Health System, Los Angeles, CA, United States.

Angiotensin II type I receptor (AT1R) is a critical player in regulating vasoconstriction, blood pressure, sodium retention. Even though AT1R has limited polymorphism, AT1R antibodies have been detected in several diseases. The role of AT1R antibodies in transplantation is first reported in kidney transplant, and then identified in heart and lung transplants. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.04.007DOI Listing
April 2019
1 Read

Non-canonical B cell functions in transplantation.

Hum Immunol 2019 Apr 10. Epub 2019 Apr 10.

Departments of Surgery and of Microbiology & Immunology, University of Michigan, Ann Arbor, MI, United States.

B cells are differentiated to recognize antigen and respond by producing antibodies. These activities, governed by recognition of ancillary signals, defend the individual against microorganisms and the products of microorganisms and constitute the canonical function of B cells. Despite the unique differentiation (e. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01988859183108
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http://dx.doi.org/10.1016/j.humimm.2019.04.006DOI Listing
April 2019
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A reverse-engineering strategy utilizing the integration of single antigen beads and NGS HLA genotypes to detect potential antibody inducing epitopes.

Hum Immunol 2019 Apr 10. Epub 2019 Apr 10.

Histocompatibility, Immunogenetics & Disease Profiling Laboratory, Stanford Blood Center, Palo Alto, CA, USA; Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA. Electronic address:

Central to the idea of antibody recognition is some degree of foreignness of the target antigen compared to the antibody producer. Epitopes are distinct regions on an antigen to which antibody can be elicited and bound. However, for HLA antigens, there is no consensus definition of what represents the minimal functional immunogenic unit of dissimilarity. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.04.002DOI Listing
April 2019
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Deep targeted sequencing reveals the diversity of TRB-CDR3 repertoire in patients with preeclampsia.

Hum Immunol 2019 Apr 6. Epub 2019 Apr 6.

Department of Genetics, National Research Institute for Family Planning, Beijing, 100081, China; Graduate School of Peking Union Medical College, Beijing, 100730, China. Electronic address:

Preeclampsia (PE) is one of the major causes of maternal and perinatal mortality worldwide. This study aimed to determine the immunological characteristics of PE patients and normal pregnancy at the T cell receptor beta-chain (TRB) level by using high-throughput sequencing. High-throughput sequencing was performed to analyze the expression of TRB-CDR3 in circulating T cells. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.04.003DOI Listing
April 2019
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Complete nucleotide sequence characterization of DRB5 alleles reveals a homogeneous allele group that is distinct from other DRB genes.

Hum Immunol 2019 Apr 5. Epub 2019 Apr 5.

Stanford Blood Center, Stanford University School of Medicine, Palo Alto, CA 94304, USA. Electronic address:

Next Generation Sequencing allows for testing and typing of entire genes of the HLA region. A better and comprehensive sequence assessment can be achieved by the inclusion of full gene sequences of all the common alleles at a given locus. The common alleles of DRB5 are under-characterized with the full exon-intron sequence of two alleles available. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.04.001DOI Listing
April 2019
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Endoplasmic reticulum aminopeptidases as a double-faced tool to increase or decrease efficiency of antigen presentation in health and disease.

Hum Immunol 2019 Mar 27. Epub 2019 Mar 27.

National Centre for Scientific Research Demokritos, Agia Paraskevi, Athens, Greece.

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https://linkinghub.elsevier.com/retrieve/pii/S01988859193029
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http://dx.doi.org/10.1016/j.humimm.2019.03.011DOI Listing
March 2019
4 Reads

Anti-vimentin antibodies in transplant and disease.

Hum Immunol 2019 Mar 26. Epub 2019 Mar 26.

Division of Surgery, Section of Transplantation, Albany Medical Center, Albany, NY, United States. Electronic address:

Non-HLA antibodies are recognized as a potential source of antibody mediated rejection following transplantation. The epitopes which lead to production of these antibodies are a result of tissue disruption, specifically endothelium, secondary to inflammation and injury. Vimentin is a cytoskeletal protein involved in many aspects of cellular organization, signaling, and proliferation. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.03.017DOI Listing
March 2019
1 Read

Accuracy of NGS HLA typing data influenced by STR.

Hum Immunol 2019 Mar 26. Epub 2019 Mar 26.

GenDx, Yalelaan 48, 3584CM Utrecht, the Netherlands. Electronic address:

Next Generation Sequencing (NGS) has become a major technology in HLA typing. The expectations are that highly accurate and unambiguous typing results will be obtained. However, HLA typing by NGS has some limitations caused by imperfections in the PCR amplification. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.03.007DOI Listing

Influence of mode of delivery on cytokine expression in cord blood.

Hum Immunol 2019 Mar 26. Epub 2019 Mar 26.

Danone Nutricia Research, Singapore; Utrecht Institute for Pharmaceutical Sciences, Utrecht University, the Netherlands. Electronic address:

The mode of delivery is a known risk factor for immune-related disorders. Normal term vaginal delivery is an inflammatory process and several cytokines are suggested to be involved. The purpose of the study was to evaluate differences in cord blood cytokine expression between modes of delivery in term-born children. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.03.018DOI Listing
March 2019
2 Reads
2.138 Impact Factor

Angiotensin II type I receptor antibodies in pediatric solid organ transplant.

Hum Immunol 2019 Mar 26. Epub 2019 Mar 26.

University of California Los Angeles, Department of Pathology and Laboratory Medicine, United States.

Minimizing immunologic complications is critical for long-term patient survival in pediatric solid organ transplant recipients. Multiple factors distinguish pediatric from adult organ transplant recipients which may influence the risk and manifestations of immunologic responses. Angiotensin II type 1 receptor antibody (AT1R-Ab) is a non-HLA antibody that has been has been associated with poor clinical outcomes in adult kidney transplant recipients. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.03.016DOI Listing

Patients with immunological diseases or on peritoneal dialysis are prone to false positive flow cytometry crossmatch.

Hum Immunol 2019 Mar 20. Epub 2019 Mar 20.

Multi-Organ Transplant Program, London Health Sciences Centre - University Hospital, London, Ontario, Canada; Department of Medicine, Schulich School of Medicine and Dentistry, Western University, London, Ontario, Canada.

Despite implementation of virtual crossmatches, flow cytometry crossmatches (FCXM) are still used by many transplant centers to determine immunological risk before kidney transplantation. To determine if common profiles of patients prone to false positive FCXM exist, we examined the demographics and native diseases of kidney patients tested with autologous FCXM (n = 480). Improvements to FCXM and cell isolation methods significantly reduced the positive rate from 15. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.03.015DOI Listing
March 2019
3 Reads

Increased frequency and FOXP3 expression of human CD8CD25 T lymphocytes and its relation to CD4 regulatory T cells in patients with hepatocellular carcinoma.

Hum Immunol 2019 Mar 20. Epub 2019 Mar 20.

Department of Microbiology and Immunology, Faculty of Medicine, Assiut University, Egypt; Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, USA.

The mechanism of action of CD8CD25FOXP3 T cells in hepatocellular carcinoma (HCC) has not been fully understood. Herein, the role of CD8CD25FOXP3 T cells in HCC was compared with that of CD4CD25FOXP3 regulatory T cells (conventional Tregs). Thirty-five patients with HCC and twenty age and sex-matched healthy adults (controls) were enrolled. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01988859183103
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http://dx.doi.org/10.1016/j.humimm.2019.03.014DOI Listing
March 2019
10 Reads

Evaluation of Th1 and Th2 immune response in clinical and sub-clinical scrub typhus infection.

Hum Immunol 2019 Mar 20. Epub 2019 Mar 20.

Medical Entomology, Arbovirology and Rickettsial Disease Division, Indian Council of Medical Research- Regional Medical Research Centre, N.E. Region, Post box no. 105, Dibrugarh-786001, Assam, India. Electronic address:

Scrub typhus (ST), caused by a gram negative intracellular bacteria- Orientia tsutsugamushi, is one among the leading causes of febrile illness across Southeast Asia, including India. Clinical presentation can vary from asymptomatic to severely fatal. Th1-cell mediated immunity has been suggested to play an important role against ST infection in animal models. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.03.013DOI Listing
March 2019
2 Reads

The role of donor-derived exosomes in lung allograft rejection.

Hum Immunol 2019 Mar 18. Epub 2019 Mar 18.

Norton Thoracic Institute, St. Joseph's Hospital and Medical Center, Phoenix, AZ, United States. Electronic address:

Lung transplant recipients (LTxRs) with acute or chronic rejection release circulating exosomes that mostly originate from donor lung tissue and express mismatched human leucocyte antigens (HLA) and lung-associated self-antigens (SAgs), Collagen-V and K alpha 1 Tubulin. During lung transplant (LTx), donor lungs often undergo injuries that increase the antigenicity of the transplanted organ. 30% of LTxRs also have pre-transplant antibodies (Abs) to HLA and lung SAgs, which may induce conditions that increase the risk of chronic lung allograft dysfunction (CLAD). Read More

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http://dx.doi.org/10.1016/j.humimm.2019.03.012DOI Listing
March 2019
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HLA-A, -B, -C, -DRB1 and -DQB1 allele and haplotype frequencies of 1463 umbilical cord blood units typed in high resolution from Bogotá, Colombia.

Hum Immunol 2019 Mar 9. Epub 2019 Mar 9.

Cord Blood Bank, Instituto Distrital de Ciencia, Biotecnología e Innovación en Salud (IDCBIS), Cra. 32 # 12-81, Bogotá, Colombia. Electronic address:

Allele and haplotype frequencies were calculated from 1463 umbilical cord blood (UCB) units, from Bogotá (Colombia) donors, HLA-typed in high resolution. This is the first report using allele-level typed colombian samples of five HLA loci related to hematopoietic stem cell transplantation (HLA-A, -B, -C, -DRB1 and -DQB1). The most frequent haplotype found in our sample was A24:02g ∼ B35:43g ∼ C01:02g ∼ DRB104:07g ∼ DQB103:02g (4. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01988859183099
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http://dx.doi.org/10.1016/j.humimm.2019.03.006DOI Listing
March 2019
3 Reads

Killer immunoglobulin like receptor gene profiling in Western Indian population.

Hum Immunol 2019 Mar 8. Epub 2019 Mar 8.

HLA & Immunogenetics Laboratory, Department of Transfusion Medicine, Tata Memorial Hospital, Parel, Mumbai 400012, India. Electronic address:

One hundred and sixty one healthy unrelated hematopoietic stem cell transplant donors from Western Indian were enrolled in this study. The study was initiated to observe the genotypic diversity of 16 killer immunoglobulin like receptor (KIR) genes in this population. KIR genotyping was carried out using the PCR-SSP technique. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.03.010DOI Listing
March 2019
2.138 Impact Factor

PD1 and PDL1 molecules control suppressor activity of regulatory T cells in chronic Chagas cardiomyopathy patients.

Hum Immunol 2019 Mar 7. Epub 2019 Mar 7.

Laboratório de Biologia das Interações Celulares, Departamento de Morfologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address:

Introduction: Chagas disease, caused by the protozoan Trypanosoma cruzi (T. cruzi), is the fourth most important tropical disease, which affects approximately 7 million people worldwide. The mechanisms involved in the development of this disease are not completely well understood. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.03.009DOI Listing

Expanded Peripheral CD4CD28T Cells and its Association with Atherosclerotic Changes in Patients with End Stage Renal Disease on Hemodialysis.

Hum Immunol 2019 Mar 7. Epub 2019 Mar 7.

Department of Internal Medicine and Nephrology, Theodor Bilharz Research Institute, Egypt.

End-stage renal disease [ESRD] patients, including those on hemodialysis, possess a high risk for cardiovascular diseases, as the first leading cause of death among them. Traditional risk factors do not utterly elucidate this. Throughout the last two decades,CD4+CD28null T cells; an unusual subset of T lymphocytes, was detected high with excess cardiovascular (CV) mortality. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.03.008DOI Listing

New HLA alleles discovered by next generation sequencing in routine histocompatibility lab work in a medium-volume laboratory.

Hum Immunol 2019 Mar 5. Epub 2019 Mar 5.

HLA/Immunogenetics and Immunodiagnostics Laboratories, Department of Pathology, Wake Forest School of Medicine, Winston-Salem, NC 27103, United States. Electronic address:

The immunogenetics research and clinical communities are undergoing a revolution in the way that Human Leukocyte Antigens (HLA) alleles are typed, thanks to the introduction and increasing acceptance of next-generation sequencing into laboratory practice. With the ability to sequence all exons of each allele, instead of the previously routine typing of exons 2 and 3 of class I and exon 2 of class II, the sequencing of previously unsequenced areas of HLA alleles is causing a host of new alleles to be discovered through the course of routine laboratory testing. In the first 4 months of routine next generation sequencing, we have identified 10 novel alleles that have been discovered through laboratory testing for all facets of HLA typing, i. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.03.005DOI Listing
March 2019
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B cells in chronic graft-versus-host disease.

Hum Immunol 2019 Mar 5. Epub 2019 Mar 5.

Department of Medicine, Duke University, Durham, NC, USA; Division of Hematologic Malignancies and Cellular Therapy, Duke University, Durham, NC, USA; Duke Cancer Institute, Duke University, Durham, NC, USA. Electronic address:

Allogeneic hematopoietic stem cell transplantation (alloHCT) is the definitive therapy for numerous otherwise incurable hematologic malignancies and non-malignant diseases. The genetic disparity between donor and recipient both underpins therapeutic effects and confers donor immune system-mediated damage in the recipient, called graft-versus-host disease (GVHD). Chronic GVHD (cGVHD) is a major cause of late post-transplant morbidity and mortality. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.03.003DOI Listing

ERAP1 shapes just part of the immunopeptidome.

Authors:
Arie Admon

Hum Immunol 2019 Mar 5. Epub 2019 Mar 5.

Faculty of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel. Electronic address:

ERAP1 is an aminopeptidase involved in trimming long peptides to the lengths required for presentation by MHC class I. ERAP1 substrate preference is for peptides with hydrophobic or aliphatic N-terminal amino acids, with lower efficacy with charged and small hydrophilic amino acids and almost complete inefficiency with proline. Since ERAP1 efficiently trims peptides to eight amino acids or even shorter, and many MHC-I allotypes can only bind peptides that are eight or nine amino acids or longer, ERAP1 both produces and destroys potential ligands of these alleles. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.03.004DOI Listing
March 2019
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Next-generation HLA typing of 382 International Histocompatibility Working Group reference B-Lymphoblastoid cell lines: report from the 17 International HLA and Immunogenetics Workshop.

Hum Immunol 2019 Mar 4. Epub 2019 Mar 4.

Department of Pathology, Stanford University School of Medicine, Palo Alto, CA, USA; Histocompatibility, Immunogenetics and Disease Profiling Laboratory, Stanford Blood Center, Palo Alto CA, USA.

Extended molecular characterization of HLA genes in the IHWG reference B-lymphoblastoid cell lines (B-LCLs) was one of the major goals for the 17 International HLA and Immunogenetics Workshop (IHIW). Although reference B-LCLs have been examined extensively in previous workshops complete high-resolution typing was not completed for all the classical class I and class II HLA genes. To address this, we conducted a single-blind study where select panels of B-LCL genomic DNA samples were distributed to multiple laboratories for HLA genotyping by next-generation sequencing methods. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01988859193022
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http://dx.doi.org/10.1016/j.humimm.2019.03.001DOI Listing
March 2019
7 Reads
2.138 Impact Factor

The role of B cell immunity in VCA graft rejection and acceptance.

Hum Immunol 2019 Mar 2. Epub 2019 Mar 2.

Transplantation Biology, Dept Microbiology & Immunology, University of Michigan, 1150 West Medical Center Drive, Ann Arbor, MI 48109, United States.

Vascularized composite allotransplantation (VCA) has emerged as the most recent field of transplantation to offer an alternative treatment for those patients that have failed or are not suitable candidates for conventional therapy. Most of the current clinical experience in this field is with recipients of skin containing grafts such as the face, upper extremity and abdominal wall transplants. Like solid organ recipients, VCA recipients require lifelong systematic immunosuppression to maintain their grafts. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.03.002DOI Listing
March 2019
1 Read

The pattern of HLA-A, -B and -DRB1 alleles and haplotypes of four Malay sub-ethnic groups namely Kelantan, Champa, Patani and Mandailing Malays of Peninsular Malaysia.

Hum Immunol 2019 Mar 2. Epub 2019 Mar 2.

Human Identification Unit, School of Health Sciences, Universiti Sains Malaysia, Health Campus, 16150 Kubang Kerian, Kelantan, Malaysia.

"Bumiputra" or "son of the soil" is a term used to represent the Malays and other indigenous populations of Malaysia. The Malays are Austronesian speaking population and originated from different parts of the Indo-Malay Archipelago. The migration of Malay population from different parts of Indo-Malay Archipelago were mainly due to trading purposes which shaped the current Malay sub-ethnic groups with unique culture and with distinctive dialects. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.02.015DOI Listing

Regulation of ERAP1 and ERAP2 genes and their disfunction in human cancer.

Hum Immunol 2019 Feb 27. Epub 2019 Feb 27.

Paediatric Haematology/Oncology Department, Ospedale Pediatrico Bambino Gesù, 00146 Rome, Italy. Electronic address:

The endoplasmic reticulum (ER) aminopeptidases ERAP1 and ERAP2 are two multifunctional enzymes playing an important role in the biological processes requiring trimming of substrates, including the generation of major histocompatibility complex (MHC) class I binding peptides. In the absence of ERAP enzymes, the cells exhibit a different pool of peptides on their surface which can promote both NK and CD8 T cell-mediated immune responses. The expression of ERAP1 and ERAP2 is frequently altered in tumors, as compared to their normal counterparts, but how this affects tumor growth and anti-tumor immune responses has been little investigated. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.02.014DOI Listing
February 2019
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The role of ERAP1 in autoinflammation and autoimmunity.

Hum Immunol 2019 Feb 25. Epub 2019 Feb 25.

Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, United States; Department of Pediatrics, College of Osteopathic Medicine, Michigan State University, East Lansing, MI 48824, United States. Electronic address:

Autoimmune and autoinflammatory diseases affect millions worldwide. These classes of disease involve abnormal immune activation of both the innate and adaptive immune systems. While both classes of disease represent a spectrum of aberrant immune activation, excessive activation of the innate immune system has been considered causal for the inflammation and tissue damage found in autoinflammatory diseases, while excessive activation of the adaptive immune system has been thought to primarily contribute to end-organ symptoms noted in autoimmune diseases. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.02.013DOI Listing
February 2019

Prevalence of autoimmune diseases and clinical significance of autoantibody profile: Data from National Institute of Hygiene in Rabat, Morocco.

Hum Immunol 2019 Feb 23. Epub 2019 Feb 23.

Laboratory of Human Pathologies Biology, Department of Biology, Faculty of Sciences, and Genomic Center of Human Pathologies, Faculty of Medicine and Pharmacy, Mohammed V University in Rabat, Morocco.

Aim: The objective of this study was to explore the prevalence of various autoimmune diseases (AIDs) in a large cohort of patients and to characterize the autoantibody profile in the patients with and without AIDs to confirm the diagnosis and to refine the Moroccan databases.

Patients And Method: Retrospective study was conducted in the Laboratory of autoimmunity National Institute of Hygiene (NIH) of Rabat in Morocco. A total of 3182 consecutive Moroccan patients (2183 females and 999 males) whose sera were tested for 14 autoantibody profile between 2010 and 2016. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.02.012DOI Listing
February 2019
5 Reads

ERAP, KIR and HLA-C gene interaction in susceptibility to recurrent spontaneous abortion in the Polish population.

Hum Immunol 2019 Feb 21. Epub 2019 Feb 21.

Laboratory of Immunogenetics and Tissue Immunology, Department of Clinical Immunology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, ul. Rudolfa Weigla 12, 53-114 Wrocław, Poland. Electronic address:

Endoplasmic reticulum aminopeptidases ERAP1 and ERAP2 trim peptides to generate stable antigenic epitopes for their presentation by HLA class I (HLA-I) molecules to T cell receptor. By influencing the peptide repertoire of HLA-I molecules, they affect also the interactions of HLA-I with killer cell immunoglobulin-like receptors (KIRs) of natural killer (NK) cells. HLA-C is the only polymorphic HLA-I molecule present on the trophoblast. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.02.010DOI Listing
February 2019
3 Reads

Influence of ERAP1 and ERAP2 gene polymorphisms on disease susceptibility in different populations.

Hum Immunol 2019 Feb 21. Epub 2019 Feb 21.

Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, China; Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Disease, Kunming 650118, China. Electronic address:

The endoplasmic reticulum aminopeptidases (ERAPs), ERAP1 and ERAP2, makes a role in shaping the HLA class I peptidome by trimming peptides to the optimal size in MHC-class I-mediated antigen presentation and educating the immune system to differentiate between self-derived and foreign antigens. Association studies have shown that genetic variations in ERAP1 and ERAP2 genes increase susceptibility to autoimmune diseases, infectious diseases, and cancers. Both ERAP1 and ERAP2 genes exhibit diverse polymorphisms in different populations, which may influence their susceptibly to the aforementioned diseases. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.02.011DOI Listing
February 2019

ERAP1-ERAP2 haplotypes are associated with ankylosing spondylitis in Polish patients.

Hum Immunol 2019 Feb 19. Epub 2019 Feb 19.

Laboratory of Immunogenetics and Tissue Immunology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland. Electronic address:

The objective of this case-control study was to evaluate the role of four single-nucleotide polymorphisms in the ERAP1 (rs2287987, rs30187, rs27044) and ERAP2 (rs2248374) genes and their haplotypes in predicting the risk for ankylosing spondylitis (AS) on a well-defined Polish population. Our study confirmed the strong association between the HLA-B*27 allele and the disease. For all tested ERAP1 SNPs we found significant differences in the minor allele and genotype distribution between patients and controls. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.02.004DOI Listing
February 2019
2 Reads

Pretransplant HLA typing revealed loss of heterozygosity in the major histocompatibility complex in a patient with acute myeloid leukemia.

Hum Immunol 2019 Apr 19;80(4):257-262. Epub 2019 Feb 19.

Indiana University School of Medicine, Department of Medicine, Division of Hematology and Oncology, Bone Marrow and Stem Cell Transplantation Program, Indiana University, Indianapolis, IN, United States.

Introduction: Chromosomal abnormalities are frequent events in hematological malignancies. The degree of HLA compatibility between donor and recipient in hematopoietic stem cell transplantation is critical.

Purpose Of The Study: In this report, we describe an acute myeloid leukemia case with loss of heterozygosity (LOH) encompassing the entire HLA. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01988859183120
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http://dx.doi.org/10.1016/j.humimm.2019.02.009DOI Listing
April 2019
8 Reads
2.138 Impact Factor

Infection with a virus generates a polyclonal immune response with broad alloreactive potential.

Hum Immunol 2019 Feb 26;80(2):97-102. Epub 2018 Oct 26.

Department of Immunohaematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands.

Virus-specific T cells have been shown to cross-react with allogeneic HLA (allo-HLA) at a clonal level. However, the impact of a single virus on the allorepertoire has never been investigated at the polyclonal level. We made an inventory of the incidence and specificity of allo-HLA-cross-reactive-virus-specific CD8 T cells in 24 healthy individuals. Read More

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http://dx.doi.org/10.1016/j.humimm.2018.10.014DOI Listing
February 2019
1 Read

HLA-F displays highly divergent and frequent haplotype lineages associated with different mRNA expression levels.

Hum Immunol 2019 Feb 26;80(2):112-119. Epub 2018 Oct 26.

São Paulo State University (UNESP), Molecular Genetics and Bioinformatics Laboratory, Experimental Research Unit (UNIPEX), School of Medicine, Botucatu, State of São Paulo, Brazil; São Paulo State University (UNESP), Pathology Department, School of Medicine, Botucatu, State of São Paulo, Brazil. Electronic address:

HLA-F is one of the most conserved loci among the HLA gene family. The exact function of HLA-F is still under investigation. HLA-F might present tolerogenic features, participate in the stabilization of HLA molecules in open conformation, and also participate in the recycling of HLA molecules. Read More

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http://dx.doi.org/10.1016/j.humimm.2018.10.016DOI Listing
February 2019

MicroRNA layer of MHC in infectious diseases.

Hum Immunol 2019 Apr 12;80(4):243-247. Epub 2019 Feb 12.

Human and Medical Genetics Laboratory, Federal University of Pará, Brazil; Programa de Pós-Graduação em Biologia de Agentes Infecciosos e Parasitários, Brazil. Electronic address:

The Major Histocompatibility Complex (MHC) harbors key genes of the immune response that are likely useful as biomarkers for infectious diseases. However, little is known about their microRNAs and what role they play in infections. The present study aimed to describe the miRNA genes in the MHC (MHC-miRNA), their variability and associations with infectious diseases. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.02.006DOI Listing
April 2019
1 Read

IgMCD27 B cells possessed regulatory function and represented the main source of B cell-derived IL-10 in the synovial fluid of osteoarthritis patients.

Hum Immunol 2019 Apr 12;80(4):263-269. Epub 2019 Feb 12.

Department of Orthopedics, Shandong Qianfoshan Hospital, Shandong University, Jinan, Shandong, China. Electronic address:

Synovial inflammation is observed in patients with osteoathritis (OA) and likely contributed to its exacerbation. Regulatory B (Breg) cells are shown to suppress inflammation in various diseases, including rheumatoid arthritis (RA). To examine whether Breg cells also participated in OA, we examined the synovial fluid from OA patients, and compared with that in RA patients. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.02.007DOI Listing
April 2019
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A multiepitopic theoretical fusion construct based on in-silico epitope screening of known vaccine candidates for protection against wide range of enterobacterial pathogens.

Hum Immunol 2019 Feb 12. Epub 2019 Feb 12.

Department of Microbiology, Panjab University, Chandigarh, India. Electronic address:

Enterobacterial pathogens that have acquired antibiotic resistance genes are a leading cause of community and hospital acquired infections. In such a situation vaccination is considered as a better option to prevent such infections. In the current study reverse vaccinology approach has been used to select peptides from already known immunogenic proteins to design a chimeric construct. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.02.008DOI Listing
February 2019
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High-resolution characterization of allelic and haplotypic HLA frequency distribution in a Spanish population using high-throughput next-generation sequencing.

Hum Immunol 2019 Feb 11. Epub 2019 Feb 11.

Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.

Next-generation sequencing (NGS) at the HLA-A, -B, -C, -DPA1, -DPB1, -DQA1, -DQB1, -DRB1 and -DRB3/4/5 loci was performed on 282 healthy unrelated individuals from different major regions of Spain. High-resolution HLA genotypes defined by full sequencing of class I loci and extended coverage of class II loci were obtained to determine allele frequencies and also to estimate extended haplotype frequencies. HLA alleles were typed at the highest resolution level (4-field level, 4FL); with exception of a minor deviation in HLA-DPA1, no statistically significant deviations from expected Hardy Weinberg Equilibrium (HWE) proportions were observed for all other HLA loci. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.02.005DOI Listing
February 2019
2 Reads

Genotyping of Italian patients with Behçet syndrome identified two novel ERAP1 polymorphisms using sequencing-based approach.

Hum Immunol 2019 02 8. Epub 2019 Feb 8.

Rheumatology Institute of Lucania (IReL) and Rheumatology Department of Lucania, San Carlo Hospital of Potenza, via Potito Petrone, Potenza 85100, Italy; Electronic address:

The endoplasmic reticulum aminopeptidase protein 1 gene (ERAP1) is related to several human diseases, including Behçet syndrome (BS), a multisystemic disorder with unknown etiology. ERAP1 is involved in immune response and its role can be influenced by gene single nucleotide variations (SNVs). We genotyped the ERAP1 whole structure in 50 consecutive BS patients and 50 ethnically-matched healthy controls using both bioinformatics and molecular methodologies. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.02.003DOI Listing
February 2019
2 Reads

Quality control project of NGS HLA genotyping for the 17th International HLA and Immunogenetics Workshop.

Hum Immunol 2019 Apr 6;80(4):228-236. Epub 2019 Feb 6.

Histocompatibility, Immunogenetics, and Disease Profiling Laboratory, Stanford Blood Center, Palo Alto, CA, USA; Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.

The 17th International HLA and Immunogenetics Workshop (IHIW) organizers conducted a Pilot Study (PS) in which 13 laboratories (15 groups) participated to assess the performance of the various sequencing library preparation protocols, NGS platforms and software in use prior to the workshop. The organizers sent 50 cell lines to each of the 15 groups, scored the 15 independently generated sets of NGS HLA genotyping data, and generated "consensus" HLA genotypes for each of the 50 cell lines. Proficiency Testing (PT) was subsequently organized using four sets of 24 cell lines, selected from 48 of 50 PS cell lines, to validate the quality of NGS HLA typing data from the 34 participating IHIW laboratories. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.01.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446570PMC
April 2019
17 Reads
2.138 Impact Factor

Towards the identification of the relative immunogenicity of individual HLA antibody epitopes.

Hum Immunol 2019 Apr 5;80(4):218-220. Epub 2019 Feb 5.

Leiden University Medical Center, Dept. Immunohaematology and Blood Transfusion, Albinusdreef 2, 2333 ZA Leiden, the Netherlands. Electronic address:

Recent data suggest that HLA epitope matching is more predictive for the formation of donor specific antibodies (DSA) after transplantation than classical HLA antigen matching. A proper definition of the epitopes and their immunogenicity is crucial for a broad clinical application of epitope matching in solid organ transplantation. For that purpose, a novel epitope component was started during the 17th International Histocompatibility and Immunogenetics Workshop. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01988859193014
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http://dx.doi.org/10.1016/j.humimm.2019.02.002DOI Listing
April 2019
1 Read

Tools for building, analyzing and evaluating HLA haplotypes from families.

Hum Immunol 2019 Feb 5. Epub 2019 Feb 5.

Histocompatibility, Immunogenetics & Disease Profiling Laboratory, Stanford Blood Center, Palo Alto, CA, USA; Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.

The highly polymorphic classical human leukocyte antigen (HLA) genes display strong linkage disequilibrium (LD) that results in conserved multi-locus haplotypes. For unrelated individuals in defined populations, HLA haplotype frequencies can be estimated using the expectation-maximization (EM) method. Haplotypes can also be constructed using HLA allele segregation from nuclear families. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.01.010DOI Listing
February 2019
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Strategies for the measurements of expression levels and half-lives of HLA class I allotypes.

Hum Immunol 2019 Apr 5;80(4):221-227. Epub 2019 Feb 5.

Department of Laboratory Medicine and Pathology, Mayo Clinic, 5777 E. Mayo Blvd, Phoenix, AZ 85054, USA.

HLA class I molecules are highly polymorphic cell surface proteins that trigger immune responses by CD8 T cells and natural killer (NK) cells. Most humans express six different HLA class I proteins encoded by the HLA-A, HLA-B and HLA-C genes. HLA class I molecules bind to peptide antigens and present these antigens to T cell receptors (TCR) of CD8 T cells. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.02.001DOI Listing

Immunotherapy in pediatric B-cell acute lymphoblastic leukemia.

Hum Immunol 2019 Feb 1. Epub 2019 Feb 1.

Division of Pediatric Hematology/Oncology, Department of Pediatric and Adolescent Medicine, Mayo Clinic, 200 First St. SW, Rochester, MN 55905, United States; Department of Immunology, Mayo Clinic, 200 First St. SW, Rochester, MN 55905, United States. Electronic address:

Advances in multi-agent chemotherapy and supportive care have dramatically improved survival of children with B-cell acute lymphoblastic leukemia (B-ALL); however, patients with relapsed and refractory disease continue to represent a therapeutic challenge. Hematopoietic stem cell transplant was the first immunotherapeutic approach to be used in the treatment of patients with relapsed or refractory disease. However, novel therapies such as bispecific antibodies that engage T-cells and chimeric antigen receptor T-cells (CAR-T) therapy have emerged as novel FDA-approved options that have the potential to become the new standard of care for these difficult-to-treat leukemias. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01988859183118
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http://dx.doi.org/10.1016/j.humimm.2019.01.011DOI Listing
February 2019
2 Reads
2.138 Impact Factor

Molecular events contributing to successful pediatric cardiac transplantation in HLA sensitized recipients.

Hum Immunol 2019 Apr 30;80(4):248-256. Epub 2019 Jan 30.

Norton Thoracic Institute, St. Joseph's Hospital and Medical Center, Phoenix, AZ, United States. Electronic address:

Antibodies to HLA resulting in positive cytotoxicity crossmatch are generally considered a contraindication for cardiac transplantation. However, cardiac transplantations have been performed in children by reducing the Abs and modifying immunosuppression. To identify mechanisms leading to allograft acceptance in the presence of Abs to donor HLA, we analyzed priming events in endothelial cells (EC) by incubating with sera containing low levels of anti-HLA followed by saturating concentration of anti-HLA. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.01.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6426665PMC
April 2019
1 Read

Exploring the ancestry and admixture of Mexican Oaxaca Mestizos from Southeast Mexico using next-generation sequencing of 11 HLA loci.

Hum Immunol 2019 Mar 29;80(3):157-162. Epub 2019 Jan 29.

Dept. of Immunology & Immunogenetics, InDRE, Secretary of Health, Mexico City, Mexico; Fundación Comparte Vida, A.C., Mexico City, Mexico. Electronic address:

The Mestizos of Oaxaca resulted from the admixture of Zapotecan Natives with Spaniards and Africans. We selected 112 donors from Oaxaca and applied next-generation sequencing to characterize exon and intron variants in complete or extended HLA genes. Some alleles found, are unique to Mexican Natives and most likely will be absent in most major ethnicities, namely: Caucasians, Africans or Asians. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.01.004DOI Listing

Genetic diversity of HLA system in three populations from Zacatecas, Mexico: Zacatecas city, Fresnillo and rural Zacatecas.

Hum Immunol 2019 Jan 24. Epub 2019 Jan 24.

Department of Transplantation, Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán" (INCMNSZ), Mexico City, Mexico. Electronic address: http://www.innsz.mx.

We studied HLA class I (HLA-A, -B) and class II (HLA-DRB1, -DQB1) alleles by PCR-SSP based typing in 453 Mexicans from the state of Zacatecas living in Zacatecas city (N = 84), Fresnillo (N = 103) and rural communities (N = 266) to obtain information regarding allelic and haplotypic frequencies and their linkage disequilibrium. We find that the most frequent haplotypes for the state of Zacatecas include seven Native American most probable ancestry (A*02 ∼ B*39 ∼ DRB1*04 ∼ DQB1*03:02; A*02 ∼ B*35 ∼ DRB1*08 ∼ DQB1*04; A*24 ∼ B*39 ∼ DRB1*14 ∼ DQB1*03:01; A*02 ∼ B*35 ∼ DRB1*04 ∼ DQB1*03:02; A*24 ∼ B*35 ∼ DRB1*04 ∼ DQB1*03:02; A*68 ∼ B*35 ∼ DRB1*04 ∼ DQB1*03:02 and A*24 ∼ B*35 ∼ DRB1*08 ∼ DQB1*04) and two European MPA haplotypes (HLA ∼ A*01 ∼ B*08 ∼ DRB1*03:01 ∼ DQB1*02 and A*29 ∼ B*44 ∼ DRB1*07 ∼ DQB1*02). Admixture estimates revealed that the main genetic components in the state of Zacatecas are European (47. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.01.007DOI Listing
January 2019
1 Read