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    3145 results match your criteria Human Herpesvirus 6

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    Risk factors of human herpesvirus 6 encephalitis/myelitis after allogeneic hematopoietic stem cell transplantation.
    Transpl Infect Dis 2017 Feb 16. Epub 2017 Feb 16.
    Department of Hematology, Hokkaido University Graduate School of Medicine, Hokkaido, Japan.
    Background: Human herpesvirus 6 (HHV-6) encephalitis/myelitis is now a well-known complication after allogeneic stem cell transplantation (allo-HSCT), particularly after cord blood transplantation (CBT). In this study, we evaluated the risk factors of HHV-6 encephalitis/myelitis.

    Methods: We evaluated 253 patients who received allo-HSCT from 2007 to 2015 at our institute. Read More

    Investigations in GABAA receptor antibody-associated encephalitis.
    Neurology 2017 Feb 15. Epub 2017 Feb 15.
    From the Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) (M.S., M.P.-P., T.A., M.R.R., F.G., J.D.), University of Barcelona, Spain; University of Lausanne (UNIL) (M.S.), Switzerland; Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER) (M.P.-P., T.A., M.R.R., J.D., F.G.), Madrid, Spain; Neurology Service (T.A., J.D.), Hospital Sant Joan de Déu, Barcelona, Spain; Neurology Division, Hospital das Clínicas (M.M.S.), São Paulo University, Brazil; Hospital de Base (F.J.C.), Brasília, Brazil; Service of Neurology (M.I.B.A.) and Service of Immunology (M.R.J.B.), University Hospital of Son Espases, Mallorca, Spain; Department of Neurology (L.B., M.G.), Children's Hospital, Boston, MA; Pediatric Neurology Section (A.F.), Vall d'Hebron University Hospital, Barcelona, Spain; Hospital Nacional Edgardo Rebagliati Martins (R.L.C.O.), Lima, Peru; Department of Neurology (M.R.R., J.D.), University of Pennsylvania, Philadelphia; Service of Neurology (F.G., J.D.), Hospital Clínic; and Catalan Institution for Research and Advanced Studies (ICREA) (J.D.), Barcelona, Spain.
    Objective: To report the clinical features, comorbidities, receptor subunit targets, and outcome in patients with anti-GABAA receptor (GABAAR) encephalitis.

    Methods: Clinical study of 26 patients, including 17 new (April 2013-January 2016) and 9 previously reported patients. Antibodies to α1, β3, and γ2 subunits of the GABAAR were determined using reported techniques. Read More

    Rev Med Interne 2017 Feb 1. Epub 2017 Feb 1.
    Service de réanimation médicale, groupe hospitalier Pitié-Salpétrière, 47-83, boulevard de l'Hôpital, 75013 Paris, France.
    Acute myocarditis must be considered in patients with recent onset of cardiac failure or arrhythmia. Fulminant myocarditis is a distinct entity characterized by sudden onset of severe congestive heart failure or cardiogenic shock, usually following a flu-like illness, parvovirus B19, human herpesvirus 6, coxsackie virus and adenovirus being the most frequently viruses responsible for the disease. In this setting, early recognition of patients rapidly progressing to refractory cardiac failure and their immediate transfer to a medical-surgical center experienced in mechanical circulatory support is warranted. Read More

    Bizarre cell dysplasia of the cervix.
    J Obstet Gynaecol Res 2017 Feb;43(2):345-351
    Šikl's Department of Pathology, Medical Faculty Hospital, Charles University, Pilsen, Czech Republic.
    Aim: The aim of this study was the characterization of a new subtype of high-grade cervical squamous intraepithelial lesion (HSIL) with enlarged cells containing bizarre nuclei: so-called bizarre cell dysplasia (BCD).

    Methods: A total of 29 cervical cone biopsy samples of this type of dysplasia were studied. Multi-target polymerase chain reaction and in situ hybridization human papillomavirus (HPV) detection was performed in all cases. Read More

    Acute liver dysfunction not resulting from hepatitis viruses in immunocompetent children.
    Pediatr Int 2017 Jan 30. Epub 2017 Jan 30.
    Department of Pediatric Hepatology and Gastroenterology, Eastern Yokohama Hospital,, Kanagawa, Japan.
    Background: The objective of the present study was to clarify the roles of cytomegalovirus (CMV), Epstein-Barr virus (EBV), and human herpesvirus 6 (HHV-6) in immunocompetent children with acute liver dysfunction not resulting from hepatitis viruses.

    Methods: Sixty-eight children (median age 3 years) hospitalized as a result of acute liver dysfunction were enrolled in this study. Hepatitis A, B, and C were excluded. Read More

    Structural and Ultrastructural Alterations in Human Olfactory Pathways and Possible Associations with Herpesvirus 6 Infection.
    PLoS One 2017 10;12(1):e0170071. Epub 2017 Jan 10.
    A. Kirchenstein Institute of Microbiology and Virology, Riga Stradins University, Riga, Latvia.
    Structural and ultrastructural alterations in human olfactory pathways and putative associations with human herpesvirus 6 (HHV-6) infection were studied. The olfactory bulb/tract samples from 20 subjects with an unspecified encephalopathy determined by pathomorphological examination of the brain autopsy, 17 healthy age-matched and 16 younger controls were used. HHV-6 DNA was detected in 60, 29, and 19% of cases in these groups, respectively. Read More

    Divergent tropism of HHV-6AGS and HHV-6BPL1 in T cells expressing different CD46 isoform patterns.
    Virology 2017 Feb 2;502:160-170. Epub 2017 Jan 2.
    Department of Biomedicine, Aarhus University, Bartholins Allé 6, DK-8000 Aarhus, Denmark. Electronic address:
    CD46 is a receptor for HHV-6A, but its role as a receptor for HHV-6B is controversial. The significance of CD46 isoforms for HHV-6A and HHV-6B tropism is unknown. HHV-6AGS was able to initiate transcription of the viral genes U7 and U23 in the CD46(+)CD134(-) T-cell lines Peer, Jurkat, Molt3, and SupT1, whereas HHV-6BPL1 was only able to do so in Molt3 and SupT1, which expressed a CD46 isoform pattern different from Peer and Jurkat. Read More

    Association between human herpesvirus & human endogenous retrovirus and MS onset & progression.
    J Neurol Sci 2017 Jan 24;372:239-249. Epub 2016 Nov 24.
    Menzies Institute for Medical Research, University of Tasmania, Australia. Electronic address:
    This review discusses the role of Epstein-Barr virus (EBV), human herpesvirus 6 (HHV6) and human endogenous retroviruses (HERVs) in the onset and progression of multiple sclerosis (MS). Although EBV has been established as one of the causal factors in MS onset, its role in MS progression is still uncertain. Moreover, interactions between EBV and other risk factor on MS development still need more investment. Read More

    Human herpesvirus 6 (HHV-6B) lymphadenitis in DRESS syndrome: a lymphoma mimic.
    Histopathology 2016 Dec 23. Epub 2016 Dec 23.
    Departments of Pathology and Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut, USA.
    Aims: Lymphadenopathy, hematologic abnormalities, and constitutional symptoms are among the non-specific manifestations seen in drug rash with eosinophilia and systemic symptoms (DRESS), an uncommon but potentially fatal cutaneous adverse drug reaction. The ubiquitous human herpesvirus 6 (HHV-6) plays a unique role in the pathogenesis of DRESS, with emerging data suggesting that reactivation occurs in most cases and contributes to the clinical manifestations, including lymphadenopathy. Further, in the appropriate clinical context, demonstration of HHV-6 reactivation may lend support to a diagnosis of DRESS. Read More

    Human Herpesvirus 6 Infection Presenting as an Acute Febrile Illness Associated with Thrombocytopenia and Leukopenia.
    Case Rep Pediatr 2016 17;2016:2483183. Epub 2016 Nov 17.
    Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloška 4, SI-1000 Ljubljana, Slovenia.
    We present an infant with acute fever, thrombocytopenia, and leukopenia, coming from an endemic region for tick-borne encephalitis, human granulocytic anaplasmosis, and hantavirus infection. The primary human herpesvirus 6 infection was diagnosed by seroconversion of specific IgM and IgG and by identification of viral DNA in the acute patient's serum. The patient did not show skin rash suggestive of exanthema subitum during the course of illness. Read More

    High Prevalence of Infectious Adeno-associated Virus (AAV) in Human Peripheral Blood Mononuclear Cells Indicative of T Lymphocytes as Sites of AAV Persistence.
    J Virol 2017 Feb 31;91(4). Epub 2017 Jan 31.
    Institute of Virology, Campus Benjamin Franklin, Charité Medical School, Berlin, Germany
    Seroepidemiology shows that infections with adeno-associated virus (AAV) are widespread, but diverse AAV serotypes isolated from humans or nonhuman primates have so far not been proven to be causes of human disease. In view of the increasing success of AAV-derived vectors in human gene therapy, definition of the in vivo sites of wild-type AAV persistence and the clinical consequences of its reactivation is becoming increasingly urgent. Here, we identify the presumed cell type for AAV persistence in the human host by highly sensitive AAV PCRs developed for the full spectrum of human AAV serotypes. Read More

    The plasma virome of febrile adult Kenyans shows frequent parvovirus B19 infections and a novel arbovirus (Kadipiro virus).
    J Gen Virol 2016 Dec 24;97(12):3359-3367. Epub 2016 Oct 24.
    8​Department of Laboratory Medicine, University of California at San Francisco, CA, USA 2​Blood Systems Research Institute, San Francisco, CA, USA.
    Viral nucleic acids present in the plasma of 498 Kenyan adults with unexplained fever were characterized by metagenomics analysis of 51 sample pools. The highest to lowest fraction of plasma pools was positive for parvovirus B19 (75 %), pegivirus C (GBV-C) (67 %), alpha anellovirus (59 %), gamma anellovirus (55 %), beta anellovirus (41 %), dengue virus genotype 2 (DENV-2) (16 %), human immunodeficiency virus type 1 (6 %), human herpesvirus 6 (6 %), HBV (4 %), rotavirus (4 %), hepatitis B virus (4 %), rhinovirus C (2 %), Merkel cell polyomavirus (MCPyV; 2 %) and Kadipiro virus (2 %). Ranking by overall percentage of viral reads yielded similar results. Read More

    Cytokine and cellular responses to human herpesvirus-6B in patients with B-acute lymphoblastic leukemia.
    Microbiol Immunol 2016 Nov;60(11):770-777
    Laboratory of Transmissible Diseases and Biological Active Substances, LR99ES27, Faculty of Pharmacy, University of Monastir, Street Avicenne 5000, Monastir, Tunisia.
    Primary infection with human herpesvirus-6 (HHV-6), is followed by its lifelong persistence in the host. Most T-cell responses to HHV-6 have been characterized using peripheral blood from healthy adults; however, the role of HHV-6 infection in immune modulation has not been elucidated for some diseases. Therefore, in this study the immune response to HHV-6 infection in patients with B-acute lymphoblastic leukemia (B-ALL) was analyzed. Read More

    Transmission of chromosomally integrated human herpesvirus 6 via cord blood transplantation.
    Transpl Infect Dis 2017 Feb 25;19(1). Epub 2016 Dec 25.
    Children's Cancer Center, National Center for Child Health and Development, Tokyo, Japan.
    Chromosomally integrated human herpesvirus 6 (ciHHV-6) can be transmitted via allogeneic hematopoietic cell transplantation. To date, only a few cases have been reported. Here, we report a case identified as transmission of ciHHV-6 via cord blood transplantation. Read More

    Progress in Quantitative Viral Load Testing: Variability and Impact of the WHO Quantitative International Standards.
    J Clin Microbiol 2017 Feb 16;55(2):423-430. Epub 2016 Nov 16.
    Department of Medicine, Alpert Medical School of Brown University, Providence, Rhode Island, USA.
    It has been hoped that the recent availability of WHO quantitative standards would improve interlaboratory agreement for viral load testing; however, insufficient data are available to evaluate whether this has been the case. Results from 554 laboratories participating in proficiency testing surveys for quantitative PCR assays of cytomegalovirus (CMV), Epstein-Barr virus (EBV), BK virus (BKV), adenovirus (ADV), and human herpesvirus 6 (HHV6) were evaluated to determine overall result variability and then were stratified by assay manufacturer. The impact of calibration to international units/ml (CMV and EBV) on variability was also determined. Read More

    [Molecular tests in diagnosis of Cytomegalovirus (CMV), human herpesvirus 6 (HHV-6) and Epstein-Barr virus (EBV) using real-time PCR in HIV positive and HIV-negative pregnant women in Ouagadougou, Burkina Faso].
    Pan Afr Med J 2016 12;24:223. Epub 2016 Jul 12.
    Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), LABIOGENE UFR/SVT, Université de Ouagadougou BP 364 Ouagadougou, Burkina Faso.
    Introduction: Herpesvirus EBV, CMV and HHV-6 are viruses that evolve based on pandemic modeling and are responsible for congenital infections causing severe sequelae in infants. This study aims to determine the prevalence of CMV, EBV and HHV-6 among HIV (+) and HIV (-) pregnant women in Ouagadougou.

    Methods: In this study 200 blood plasma samples taken from pregnant women, of whom 100 with HIV(+) and 100 with HIV(-), were analyzed using multiplex real-time PCR which detected three infections (EBV, CMV and HHV-6). Read More

    HHV-6 and seizure: A systematic review and meta-analysis.
    J Med Virol 2017 Jan 9;89(1):161-169. Epub 2016 Aug 9.
    Division of Epilepsy and Clinical Neurophysiology, Department of Neurology, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts.
    Human herpesvirus-6 (HHV-6) is a common infection in childhood. This systematic review aims to assess the relationship between HHV-6 infection and seizure disorders based on available literature. MEDLINE database was searched using a search strategy. Read More

    Inherited chromosomally-integrated human herpesvirus 6 and breast cancer.
    Cancer Epidemiol Biomarkers Prev 2016 Oct 24. Epub 2016 Oct 24.
    Microbiology-infectious disease and immunology, Universite Laval
    Background Inherited chromosomally-integrated human herpesvirus 6 (iciHHV-6) is a condition observed in approximately 1% of the population. Whether such a genetic alteration predisposes to cancer development in currently unknown. Two studies were conducted to determine whether iciHHV-6 is associated with cancer development. Read More

    Human Herpesvirus 6 Infection Following Haploidentical Transplantation: Immune Recovery and Outcome.
    Biol Blood Marrow Transplant 2016 Dec 30;22(12):2250-2255. Epub 2016 Sep 30.
    Unit of Hematology and Bone Marrow Transplantation, IRCCS San Raffaele Scientific Institute, Milan, Italy; Vita-Salute San Raffaele University, Milan, Italy. Electronic address:
    Human herpesvirus 6 (HHV-6) is increasingly recognized as a potentially life-threatening pathogen in allogeneic hematopoietic stem cell transplantation (alloSCT). We retrospectively evaluated 54 adult patients who developed positivity to HHV-6 after alloSCT. The median time from alloSCT to HHV-6 reactivation was 34 days. Read More

    Distribution and effects of amino acid changes in drug-resistant α and β herpesviruses DNA polymerase.
    Nucleic Acids Res 2016 Nov 29;44(20):9530-9554. Epub 2016 Sep 29.
    Rega Institute for Medical Research, Department Microbiology and Immunology, KU Leuven, Minderbroedersstraat 10, 3000, Leuven, Belgium
    Emergence of drug-resistance to all FDA-approved antiherpesvirus agents is an increasing concern in immunocompromised patients. Herpesvirus DNA polymerase (DNApol) is currently the target of nucleos(t)ide analogue-based therapy. Mutations in DNApol that confer resistance arose in immunocompromised patients infected with herpes simplex virus 1 (HSV-1) and human cytomegalovirus (HCMV), and to lesser extent in herpes simplex virus 2 (HSV-2), varicella zoster virus (VZV) and human herpesvirus 6 (HHV-6). Read More

    Association of active human herpesvirus-6 (HHV-6) infection with autoimmune thyroid gland diseases.
    Clin Microbiol Infect 2017 Jan 30;23(1):50.e1-50.e5. Epub 2016 Sep 30.
    Riga Stradiņš University, A. Kirchenstein Institute of Microbiology and Virology, Latvia.
    Objectives: Viral infections frequently have been cited as important environmental factors implicated in the onset of autoimmune thyroiditis (AIT). The aim of this study was to determine the involvement of HHV-6 infection in the development of autoimmune thyroiditis.

    Methods: This study included 45 patients (42 female and 3 male; median age 47. Read More

    Human Herpesvirus 6 replication predicts Cytomegalovirus reactivation after allogeneic stem cell transplantation from haploidentical donor.
    J Clin Virol 2016 Nov 20;84:24-26. Epub 2016 Sep 20.
    Molecular Biology Section, Clinical Investigation Laboratory, Istituto Clinico Humanitas, Rozzano 20089, Italy. Electronic address:
    Background And Objectives: Since HHV-6 reactivation after transplant has been reported to increase the risk of CMV infection, we tested this hypothesis in the HLA-haploidentical hematopoietic stem cell transplantation setting.

    Study Design: From February 2011 to October 2015, 75 patients received hematopoietic stem cell transplantation using a T-cell replete graft from a HLA-haploidentical donor at our Institution.

    Results: Interestingly, 87% of HHV-6 reactivations were followed by a CMV reactivation, at a median of 15days between the two viruses. Read More

    Central nervous system infection following allogeneic hematopoietic stem cell transplantation.
    Hematol Oncol Stem Cell Ther 2016 Sep 17. Epub 2016 Sep 17.
    Hematology Division, Tokyo Metropolitan Cancer and Infectious Diseases Center, Komagome Hospital, Tokyo, Japan. Electronic address:
    Objective/background: Here, we described the clinical characteristics and outcomes of central nervous system (CNS) infections occurring after allogeneic hematopoietic stem cell transplantation (allo-HSCT) in a single institution over the previous 6 years.

    Methods: Charts of 353 consecutive allogeneic transplant recipients were retrospectively reviewed for CNS infection.

    Results: A total of 17 cases of CNS infection were identified at a median of 38 days (range, 10-1028 days) after allo-HSCT. Read More

    Identification of Viruses in Cases of Pediatric Acute Encephalitis and Encephalopathy Using Next-Generation Sequencing.
    Sci Rep 2016 Sep 14;6:33452. Epub 2016 Sep 14.
    Departments of Pediatrics, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
    Acute encephalitis/encephalopathy is a severe neurological syndrome that is occasionally associated with viral infection. Comprehensive virus detection assays are desirable because viral pathogens have not been identified in many cases. We evaluated the utility of next-generation sequencing (NGS) for detecting viruses in clinical samples of encephalitis/encephalopathy patients. Read More

    [Human herpes virus 6 infection in an inmunocompetent patient with carbamazepine-induced DRESS syndrome].
    Rev Chilena Infectol 2016 Jun;33(3):340-5
    Departamento de Dermatología, Facultad de Medicina, Pontificia Universidad Católica de Chile, Chile,
    DRESS syndrome (drug reaction with eosinophilia and systemic symptoms) is an adverse life-threatening drug reaction characterized by a polymorphous rash associated with fever, lymphadenopathy and multiorgan involvement with eosinophilia. We present the case of an immunocompetent man with DRESS syndrome secondary to carbamazepine, that developed concomitantly meningoencephalitis caused by human herpes virus 6 (HHV-6), and a review of literature. The pathogenic role of HHV-6 in DRESS syndrome remains controversial. Read More

    Hippocampal signal abnormality on the first day of illness in acute encephalopathy with biphasic seizures and late reduced diffusion caused by HHV-6 infection.
    Brain Dev 2016 Nov 29;38(10):943-946. Epub 2016 Aug 29.
    Department of Pediatrics, Osaka Medical College, Takatsuki, Osaka, Japan.
    We report a 13-month-old girl who developed acute encephalopathy with biphasic seizures and late reduced diffusion (AESD) with transient reduced diffusion in the hippocampus and anterior commissure on diffusion-weighted imaging (DWI), which was performed on the first day after febrile status epilepticus (FSE) as the initial neurological symptom of AESD. DWI just after late seizures showed high signal intensity lesions in both left hippocampus and anterior commissure, and left extratemporal and occipital subcortical white matter. HHV-6 DNA was positive in both blood and cerebrospinal fluid samples. Read More

    Eosinophilic drug reactions detected by a prospective pharmacovigilance programme in a tertiary hospital.
    Br J Clin Pharmacol 2017 Feb 12;83(2):400-415. Epub 2016 Oct 12.
    Department of Clinical Pharmacology, La Paz University Hospital-Carlos III, IdiPAZ, School of Medicine, Autonomous University of Madrid, Madrid, Spain.
    Aim: We conducted a prospective evaluation of all eosinophilic drug reactions (EDRs) through the Prospective Pharmacovigilance Program from Laboratory Signals at Hospital to find out the incidence and distribution of these entities in our hospital, their causative drugs, and predictors.

    Methods: All peripheral eosinophilia >700 × 10(6)  cells l(-1) detected at admission or during hospitalisation, were prospectively monitored over 42 months. The spectrum of the localised or systemic manifestation of EDR, the incidence, the distribution of causative drugs, and the predictors were analysed. Read More

    Roseolovirus-associated encephalitis in immunocompetent and immunocompromised individuals.
    J Neurovirol 2016 Aug 18. Epub 2016 Aug 18.
    Department of Medical Oncology and Hematology, Oita University Hospital, Hasama-machi, Yufu City, 879-5593, Japan.
    The roseoloviruses, human herpesvirus (HHV)-6A, HHV-6B, and HHV-7, can cause severe encephalitis or encephalopathy. In immunocompetent children, primary HHV-6B infection is occasionally accompanied by diverse clinical forms of encephalitis. Roseolovirus coinfections with heterologous viruses and delayed primary HHV-7 infection in immunocompetent adults result in very severe neurological and generalized symptoms. Read More

    Human Herpesvirus 6B Downregulates Expression of Activating Ligands during Lytic Infection To Escape Elimination by Natural Killer Cells.
    J Virol 2016 Nov 14;90(21):9608-9617. Epub 2016 Oct 14.
    Lautenberg Center for General and Tumor Immunology, BioMedical Research Institute Israel-Canada, Faculty of Medicine, The Hebrew University Hadassah Medical School, Jerusalem, Israel
    The Herpesviridae family consists of eight viruses, most of which infect a majority of the human population. One of the less-studied members is human herpesvirus 6 (HHV-6) (Roseolovirus), which causes a mild, well-characterized childhood disease. Primary HHV-6 infection is followed by lifelong latency. Read More

    Comparative Outcomes after Haploidentical or Unrelated Donor Bone Marrow or Blood Stem Cell Transplantation in Adult Patients with Hematological Malignancies.
    Biol Blood Marrow Transplant 2016 Nov 10;22(11):2047-2055. Epub 2016 Aug 10.
    John Theurer Cancer Center, Hackensack University Medical Center, Hackensack, New Jersey.
    Most patients eligible for allogeneic hematopoietic stem cell transplantation will require identification of an alternate (unrelated or mismatched related) donor. We explored the transplantation outcomes for a sequential series of 54 patients undergoing haploidentical donor transplantation (HAPLO) compared to those from a control group of patients receiving cells from matched or mismatched unrelated donors (URD) selected by diagnosis and stem cell source. Patients undergoing HAPLO transplantations received graft-versus-host disease (GVHD) prophylaxis with post-transplantation cyclophosphamide (Cy). Read More

    Pityriasis Rosea: An Update on Etiopathogenesis and Management of Difficult Aspects.
    Indian J Dermatol 2016 Jul-Aug;61(4):375-84
    Maulana Azad Medical College, New Delhi, India.
    Pityriasis rosea (PR) is a benign papulosquamous disorder seen commonly in clinical practice. Despite its prevalence and benign nature, there are still times when this common disorder presents in an uncommon way or course posing diagnostic or management problems for the treating physician. The etiopathogenesis of PR has always been a dilemma, and extensive research is going on to elicit the exact cause. Read More

    Herpesvirus Respiratory Infections in Immunocompromised Patients: Epidemiology, Management, and Outcomes.
    Semin Respir Crit Care Med 2016 Aug 3;37(4):603-30. Epub 2016 Aug 3.
    Division of Infectious Diseases, Department of Medicine, Loyola University, Maywood, Illinois.
    Among immunocompromised individuals, members of the human Herpesviridae family are frequently encountered pathogens. Cytomegalovirus, herpes simplex virus 1 and 2, varicella zoster virus, Epstein-Barr virus, and human herpesvirus-6, -7, and -8 all establish latency after infection and can reactivate during periods of immunosuppression, leading to both direct and indirect adverse effects on the host including severe organ dysfunction as well as allograft rejection and loss after transplantation. While not all herpesviruses are primary respiratory pathogens, many of their manifestations include involvement of the respiratory tract. Read More

    Human herpesvirus multiplex ddPCR detection in brain tissue from low- and high-grade astrocytoma cases and controls.
    Infect Agent Cancer 2016 26;11:32. Epub 2016 Jul 26.
    The National Institute of Neurological Disorders and Stroke, National Institutes of Health, BG 10 RM 5C103 10 Center Dr., Bethesda, MD 20892 USA.
    Background: Glioblastoma (GBM) is a fatal CNS malignancy, representing 50 % of all gliomas with approximately 12-18 months survival time after initial diagnosis. Recently, the human herpesvirus cytomegalovirus (CMV) has been suggested to have an oncogenic role, yet this association remains controversial. In addition, human herpesvirus 6 (HHV-6) and Epstein-Barr virus (EBV) have also been associated with low-grade gliomas, but few studies have examined HHV-6 and EBV in glioblastomas. Read More

    Viral load and ganciclovir (GCV) concentration in cerebrospinal fluid of patients successfully treated with GCV or valGCV for human herpesvirus 6 encephalitis/myelitis following umbilical cord blood transplantation.
    Transpl Infect Dis 2016 Oct 7;18(5):773-776. Epub 2016 Sep 7.
    Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan.
    We describe successful treatment of 3 cases of human herpesvirus 6 (HHV-6) encephalitis/myelitis following cord blood transplantation (CBT). Ganciclovir (GCV) (10 mg/kg/day) reduced HHV-6 load to undetectable levels in cerebrospinal fluid (CSF). Early dose reduction in the presence of HHV-6 detectable in CSF resulted in an increased HHV-6 load. Read More

    Non-paraneoplastic limbic encephalitis and central nervous HHV-6B reactivation: Causality or coincidence?
    Neuropathology 2016 Aug 15;36(4):376-80. Epub 2016 Jan 15.
    Department of Neurology / Pathology, Oslo University Hospital, Oslo, Norway.
    Autoantibody-related encephalopathies represent an important differential diagnosis in adult onset epilepsy. Here, we report the case of a 25-year-old patient with new-onset epilepsy and psychotic syndrome, who underwent biopsy resection for etiological classification. MRI analysis and neuropathological examination showed a T-lymphocytic dominated encephalitis with involvement of the limbic system. Read More

    A comprehensive next generation sequencing-based virome assessment in brain tissue suggests no major virus - tumor association.
    Acta Neuropathol Commun 2016 Jul 11;4(1):71. Epub 2016 Jul 11.
    Department of Pathology, Tulane University, New Orleans, LA, 70112, USA.
    Next generation sequencing (NGS) can globally interrogate the genetic composition of biological samples in an unbiased yet sensitive manner. The objective of this study was to utilize the capabilities of NGS to investigate the reported association between glioblastoma multiforme (GBM) and human cytomegalovirus (HCMV). A large-scale comprehensive virome assessment was performed on publicly available sequencing datasets from the Cancer Genome Atlas (TCGA), including RNA-seq datasets from primary GBM (n = 157), recurrent GBM (n = 13), low-grade gliomas (n = 514), recurrent low-grade gliomas (n = 17), and normal brain (n = 5), and whole genome sequencing (WGS) datasets from primary GBM (n = 51), recurrent GBM (n = 10), and normal matched blood samples (n = 20). Read More

    Human herpesvirus 6 and 7 are biomarkers for fatigue, which distinguish between physiological fatigue and pathological fatigue.
    Biochem Biophys Res Commun 2016 Sep 7;478(1):424-30. Epub 2016 Jul 7.
    Department of Psychiatry, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan.
    Fatigue reduces productivity and is a risk factor for lifestyle diseases and mental disorders. Everyone experiences physiological fatigue and recovers with rest. Pathological fatigue, however, greatly reduces quality of life and requires therapeutic interventions. Read More

    Virus reactivations after autologous hematopoietic stem cell transplantation detected by multiplex PCR assay.
    J Med Virol 2017 Feb 27;89(2):358-362. Epub 2016 Jul 27.
    Department of Pediatrics, Sapporo Medical University School of Medicine, Sapporo, Japan.
    Several studies have indicated that viral reactivations following allogeneic hematopoietic stem cell transplantation (allo-HSCT) are frequent, but viral reactivations after autologous HSCT (auto-HSCT) have not been investigated in detail. We performed multiplex polymerase chain reaction (PCR) assay to examine multiple viral reactivations simultaneously in 24 patients undergoing auto-HSCT between September 2010 and December 2012. Weekly whole blood samples were collected from pre- to 42 days post-HSCT, and tested for the following 13 viruses; herpes simplex virus 1 (HSV-1), HSV-2, varicella-zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), human herpesvirus 6 (HHV-6), HHV-7, HHV-8, adeno virus (ADV), BK virus (BKV), JC virus (JCV), parvovirus B19 (B19V), and hepatitis B virus (HBV). Read More

    Relapsing drug-induced hypersensitivity syndrome.
    Curr Opin Allergy Clin Immunol 2016 Aug;16(4):333-8
    Department of Rheumatology, Allergy and Immunology, Tan Tock Seng Hospital, Singapore, Singapore.
    Purpose Of Review: Drug-induced hypersensitivity syndrome (DIHS) is a serious adverse drug reaction with potential morbidity and mortality. 'Flare up' or relapses in DIHS is a rare but important feature. These relapses may occur within days to several weeks, even after discontinuation of the offending drug. Read More

    Transcriptome sequencing of neurologic diseases associated genes in HHV-6A infected human astrocyte.
    Oncotarget 2016 Jul;7(30):48070-48080
    Department of Immunology, Nanjing Medical University, Nanjing, Jiangsu Province, People's Republic of China.
    Human Herpesvirus 6 (HHV-6) has been involved in the development of several central nervous system (CNS) diseases, such as Alzheimer's disease, multiple sclerosis and glioma. In order to identify the pathogenic mechanism of HHV-6A infection, we carried out mRNA-seq study of human astrocyte HA1800 cell with HHV-6A GS infection. Using mRNA-seq analysis of HA1800-control cells with HA1800-HHV-6A GS cells, we identified 249 differentially expressed genes. Read More

    Multicenter Evaluation of BioFire FilmArray Meningitis/Encephalitis Panel for Detection of Bacteria, Viruses, and Yeast in Cerebrospinal Fluid Specimens.
    J Clin Microbiol 2016 Sep 22;54(9):2251-61. Epub 2016 Jun 22.
    BioFire Diagnostics, LLC, Salt Lake City, Utah, USA.
    Rapid diagnosis and treatment of infectious meningitis and encephalitis are critical to minimize morbidity and mortality. Comprehensive testing of cerebrospinal fluid (CSF) often includes Gram stain, culture, antigen detection, and molecular methods, paired with chemical and cellular analyses. These methods may lack sensitivity or specificity, can take several days, and require significant volume for complete analysis. Read More

    Cytomegalovirus and Langerhans Cell Histiocytosis: Is There a Link?
    Iran J Pediatr 2016 Apr 27;26(2):e673. Epub 2016 Feb 27.
    Department of Community Oral Health, Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, IR Iran.
    Background: Langerhans cell histiocytosis is a rare proliferative histiocytic disease of unknown etiology. Histologically, it is characterized by granuloma-like proliferation of Langerhans-type dendritic cells derived from bone marrow. Many investigators have suggested the possible role of viruses such as Epstein-Barr virus, human herpesvirus-6 (HHV-6), herpes simplex virus (HSV) types 1 and 2, and Cytomegalovirus in the pathogenesis of Langerhans cell histiocytosis. Read More

    Co-existence of histopathological features is characteristic in drug reaction with eosinophilia and systemic symptoms and correlates with high grades of cutaneous abnormalities.
    J Eur Acad Dermatol Venereol 2016 Dec 26;30(12):2077-2084. Epub 2016 May 26.
    Department of Dermatology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
    Background: Only few studies had investigated the histopathological presentations of drug reaction with eosinophilia with systemic symptoms (DRESS). The results of these studies were diverse and not conclusive. A characteristic histopathological feature is still lacking. Read More

    Caregiver burden and fatigue in caregivers of people with dementia: Measuring human herpesvirus (HHV)-6 and -7 DNA levels in saliva.
    Arch Gerontol Geriatr 2016 Sep-Oct;66:42-8. Epub 2016 May 7.
    Kobe Gakuin University Faculty of Rehabilitation, 518 Arise, Ikawadani-cho, Nishi-ku, Kobe, Hyogo 651-2180, Japan. Electronic address:
    Purpose: We examined chronic fatigue, which has not been investigated in detail, in family caregivers for people with dementia.

    Methods And Materials: Forty-four community-dwelling family caregivers (the caregiver group: CG) and 50 elderly control participants (the non-caregiver group: NCG) participated in this study. We measured salivary human herpesvirus (HHV)-6 and -7 DNA levels and the Chalder fatigue scale (CFS) to assess levels of fatigue; we also measured the Center for Epidemiologic Studies-Depression Scale, Physical Activity Scale for the Elderly, Zarit Caregiver Burden Interview, Mini-Mental State Examination, Assessment of Motor and Process Skills, and Dementia Behavior Disturbance Scale. Read More

    The putative U94 integrase is dispensable for human herpesvirus 6 (HHV-6) chromosomal integration.
    J Gen Virol 2016 Aug 11;97(8):1899-903. Epub 2016 May 11.
    1​ Institut für Virologie, Freie Universität Berlin, Robert von Ostertag-Straße 7-13, 14163 Berlin, Germany.
    Human herpesvirus 6 (HHV-6) can integrate its genome into the telomeres of host chromosomes and is present in the germline of about 1 % of the human population. HHV-6 encodes a putative integrase U94 that possesses all molecular functions required for recombination including DNA-binding, ATPase, helicase and nuclease activity, and was hypothesized by many researchers to facilitate integration ever since the discovery of HHV-6 integration. However, analysis of U94 in the virus context has been hampered by the lack of reverse-genetic systems and efficient integration assays. Read More

    Virological testing of cerebrospinal fluid in children aged less than 14 years with a suspected central nervous system infection: A retrospective study on 304 consecutive children from January 2012 to May 2015.
    Eur J Paediatr Neurol 2016 Jul 16;20(4):588-96. Epub 2016 Apr 16.
    Department of Molecular Medicine, University of Padova, Via Gabelli 63, 35100 Padova, Italy; Microbiology and Virology Unit, Padova University Hospital, Indirizzo: Via Giustiniani, 2, 35128 Padova, Italy.
    Objective: The study aimed to describe the prevalence of HSV DNA, VZV DNA, Enterovirus RNA, Parechovirus RNA, CMV DNA, EBV DNA, adenovirus DNA, HHV-6 DNA, HHV-7 DNA, HHV-8 DNA and Parvovirus B19DNA in children aged less 14 years with a suspected viral infection of the central nervous system in a clinical practice setting.

    Methods: Between January 2012 and May 2015, cerebrospinal fluids from 304 children were tested with an in-house real-time PCR method.

    Results: A positive PCR was detected in 64 subjects (21%): the mean number of tests performed in patients who showed a viral infection was 7. Read More

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