18,084,799 results match your criteria Human Brain Mapping[Journal]

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Optimizing denaturing HPLC as a robust technique for identification of Short Tandem Repeats (STR) in forensic medicine.

J Forensic Leg Med 2018 Dec 10;61:108-114. Epub 2018 Dec 10.

Department and Biotechnology Research Center, Semnan University of Medical Sciences, Semnan, Iran. Electronic address:

Introduction: Short Tandem Repeats (STRs) are defined as short lengths of 2-7 base pairs spreading through human genome which due to their highly diverse individually distribution are widely applied for identity detection and other forensic medicine purposes. Burdening considerable costs by the conventional methods such as capillary electrophoresis, we aimed to compare concomitant usage of multiplex PCR and denaturing high-performance liquid chromatography (DHPLC) as cheap, fast, highly accurate, and more accessible methods, with capillary electrophoresis (CE) to evaluate their potential for early screening of STRs.

Materials And Methods: The present study randomly included 20 blood samples from the subjects referred to forensic medicine of Semnan, Iran. Read More

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December 2018
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Differential exposure to organophosphate flame retardants in mother-child pairs.

Chemosphere 2018 Dec 4;219:567-573. Epub 2018 Dec 4.

Departmentof Environmental Health Sciences, Mailman School of Public Health, Columbia University, 722 W 168th St, New York, NY 10032, USA. Electronic address:

Background: Humans are ubiquitously exposed to flame retardants, including organophosphate esters (OPEs), through direct contact with consumer products or exposure through household dust. Children are at increased risk because of their proximity to dust, hand-to-mouth activity, and the importance of childhood as a critical period in neurodevelopment.

Objectives: To quantify differences in exposure levels between mothers and children (three to six years of age), we analyzed urinary metabolites of OPEs. Read More

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December 2018

Behavioral, molecular and physiological responses of embryo-larval zebrafish exposed to types I and II pyrethroids.

Chemosphere 2018 Dec 6;219:526-537. Epub 2018 Dec 6.

Department of Environmental Toxicology, The Institute of Environmental and Human Health, Texas Tech University, Lubbock, TX 79416, USA.

Pyrethroids are potent neurotoxicants that may elicit multiple pathways of toxicity in non-target organisms. Comparative studies on the mechanistic and developmental effects of types I and II pyrethroids against non-target aquatic species are limited. This study assessed the effects of the two pyrethroid types against embryo-larval zebrafish (Danio rerio) at environmentally relevant and laboratory concentrations. Read More

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December 2018

Urinary levels of bisphenol A, F and S and markers of oxidative stress among healthy adult men: Variability and association analysis.

Environ Int 2018 Dec 12;123:301-309. Epub 2018 Dec 12.

Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, State Key Laboratory of Environmental health (incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China; Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China. Electronic address:

Background: Bisphenol F (BPF) and bisphenol S (BPS) are increasingly used as alternatives to endocrine disrupting chemical bisphenol A (BPA). Evidence from in vitro and animal studies demonstrates that BPA, BPF and BPS induce oxidative stress, a proposed mechanism that is relevant to various adverse health effects. Evaluation in humans is hampered by the potentially high within-subject variability of urinary measurements. Read More

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December 2018

Checkpoint-modulating immunotherapies in tumor treatment: Targets, drugs, and mechanisms.

Int Immunopharmacol 2018 Dec 12;67:160-175. Epub 2018 Dec 12.

Wuya College of Innovation, Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China; Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Electronic address:

Tumor immunotherapy, as a new treatment of cancer, has been developing on the basis of tumor immunology. Tumor immunotherapy stimulates and enhances the function of immune system in human bodies, in order to control and kill tumor cells. It is often used as an adjuvant therapy combined with surgery, chemotherapy, radiotherapy and other conventional methods. Read More

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December 2018
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Therapeutic evaluation of acupoint stimulation with needle-scapelon on rat model of degenerative cervical intervertebral discs.

Biomed Pharmacother 2018 Dec 12;110:677-684. Epub 2018 Dec 12.

Department of Acupuncture, Qianfo Shan Hospital, Jinan, Shandong Province, 250014, China.

Cervical spondylosis (CS), which is resulted from degeneration of cervical intervertebral disc, is a common disease seriously threatening human health and quality of life. However, there is still no effective clinic strategies for the treatment of this disease. The acupoint stimulation with needle-scalpel is a widely used approach to treat orthopedic diseases. Read More

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December 2018

Clinicopathological characteristics of metaplastic breast cancer - analysis of the basic immunohistochemical profile and comparison with other invasive breast cancer types.

Breast 2018 Dec 7;43:135-141. Epub 2018 Dec 7.

Department of Biophysics and Human Physiology, Medical University of Warsaw, Chalubinskiego 5, 02-004 Warsaw, Poland.

Introduction: Metaplastic breast cancer (MpBC) is a rare but aggressive type of breast cancer accounting for 0.25-1% of all diagnosed invasive breast cancers. Morphologically, it is characterized by differentiation of the neoplastic epithelium into squamous cells and/or mesenchymal-looking tissue. Read More

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December 2018

Primary cultured neuronal networks and type 2 diabetes model mouse fatty liver tissues in aqueous liquid observed by atmospheric SEM (ASEM): Staining preferences of metal solutions.

Micron 2018 Nov 23;118:9-21. Epub 2018 Nov 23.

Biomedical Research Institute, National Institute of Industrial Science and Technology (AIST), Higashi 1-1-1, Tsukuba, Ibaraki, 305-8566, Japan. Electronic address:

Neural networking, including axon targeting and synapse formation, is the basis of various brain functions, including memory and learning. Diabetes-mellitus affects peripheral nerves and is known to cause fatty liver disease. Electron microscopy (EM) provides the resolution required to observe changes in fine subcellular structures caused by such physiological and pathological processes, but samples are observed in vacuum. Read More

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November 2018

Mesenchymal stromal cells isolated from human fetal liver release soluble factors with a potential role in liver tissue repair.

Differentiation 2018 Dec 5;105:14-26. Epub 2018 Dec 5.

Department of Research, IRCCS-ISMETT, Palermo, Italy.

We isolated a population of proliferating cells from cultured human fetal hepatocytes of 16-22 weeks gestational age. The cells shared a similar phenotype to that of mesenchymal stromal cells (MSCs) according to the International Society for Cellular Therapy (ISCT), including plastic adherence, antigen expression profile, and in vitro multilineage differentiation potential. Fetal liver (FL)-MSCs expressed the albumin gene, and harbored a subpopulation of CK18 cells (20-40%), which defined their hepatic origin. Read More

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December 2018
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Molecular basis of Tityus stigmurus alpha toxin and potassium channel kV1.2 interactions.

J Mol Graph Model 2018 Nov 28;87:197-203. Epub 2018 Nov 28.

Department of Pharmacy, Universidade Federal do Rio Grande do Norte (UFRN), Natal, 59010-180, Brazil. Electronic address:

The Tityus stigmurus scorpion is widely distributed in the Northeast of Brazil and is the main causal agent of human envenoming. The venom produced by this scorpion includes neurotoxins, which are peptides belonging to Family 2 toxins and are able to interact with ion channels. The KTx subfamily displays selectivity and affinity for Kv channel subtypes and the result of this interaction is the blockade of potassium channels, impairing vital functions. Read More

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November 2018

Influence of the T to S mutation at the STMK motif on antibiotic resistance of penicillin binding protein 1A: A comprehensive computational study.

J Mol Graph Model 2018 Dec 7;87:185-191. Epub 2018 Dec 7.

Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.

The emergence of antibiotic resistance has attracted the attention of scientists and scientific circles over the decades. β-Lactam antibiotics resistance is a worldwide therapeutic challenge in bacterial infections, mediated through several mechanisms of which mutations in Penicillin Binding Proteins (PBPs) are an important issue, making critical therapeutic problems in the human population. Accordingly, investigating the dynamic structures of mutant variants could result in a profound understanding of such a specific resistance. Read More

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December 2018

Influence of hypertension on brain amyloid deposition and Alzheimer's disease signature neurodegeneration.

Neurobiol Aging 2018 Nov 16;75:62-70. Epub 2018 Nov 16.

Department of Neuropsychiatry, Seoul National University Hospital, Seoul, Republic of Korea; Institute of Human Behavioral Medicine, Medical Research Center, Seoul National University, Seoul, Republic of Korea; Department of Psychiatry, Seoul National University College of Medicine, Seoul, Republic of Korea. Electronic address:

This study aimed to investigate the relationship of hypertension with beta-amyloid (Aβ) and neurodegeneration biomarkers of Alzheimer's disease (AD) and the modulating effect of apolipoprotein E-ε4 (APOE4). In total, 259 cognitively normal (CN) and 79 AD dementia older adults received clinical assessments including the evaluation for the presence of hypertension, [C]-Pittsburgh-compound-B-positron emission tomography, magnetic resonance imaging, and APOE genotyping. We used a clinical stage-specific approach, separately focusing on CN and AD dementia stages. Read More

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November 2018

The therapeutic potential of curcumin: A review of clinical trials.

Eur J Med Chem 2018 Dec 7;163:527-545. Epub 2018 Dec 7.

Phytochemistry Research Center, Shahid Beheshti University of Medical Sciences, Tehran, 11369, Iran; Department of Chemistry, Richardson College for the Environmental Science Complex, The University of Winnipeg, Winnipeg, MB, R3B 2G3, Canada. Electronic address:

Curcuma longa L., its derived extracts and even its major compound curcumin has a long history of use and doubtless effectiveness, reported through increasingly detailed in vitro, ex vivo, in vivo and even clinical trials. Regarding its biological effects, multiple health-promoting, disease-preventing and even treatment attributes has been remarkably highlighted. Read More

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December 2018
3 Reads

Attentional priming: recent insights and current controversies.

Curr Opin Psychol 2018 Nov 28;29:71-75. Epub 2018 Nov 28.

Icelandic Vision Laboratory, School of Humanitites and Social Sciences, University of Akureyri. Hafnarstræti 97, Akureyri IS600, Iceland.

Humans possess a primitive memory system for attention deployments that allows quick reorientation of visual attention to stimuli that are relevant to behavior at any given moment. We review recent evidence regarding such attentional priming effects from a number of different perspectives. We discuss recent findings on the time course and duration of such effects, the potential interaction of priming and top-down attentional guidance; how priming can be used to probe the nature of visual representations and attentional templates; findings on the basic nature of priming effects and recent relevant findings on so-called serial dependencies that share many characteristics with attentional priming. Read More

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November 2018
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Delivery of miR-212 by chimeric peptide-condensed supramolecular nanoparticles enhances the sensitivity of pancreatic ductal adenocarcinoma to doxorubicin.

Biomaterials 2018 Nov 30;192:590-600. Epub 2018 Nov 30.

Department of Hepatobiliary and Pancreatic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. Electronic address:

Pancreatic ductal adenocarcinoma (PDAC) is a destructive cancer with poor prognosis. Both novel therapeutic targets and approaches are needed to improve the overall survival of PDAC patients. MicroRNA-212 (miR-212) has been reported as a tumor suppressor in multiple cancers, but its definitive role and exact mechanism in the progression of pancreatic cancer is unclear. Read More

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November 2018
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Successful genetic modification of porcine spermatogonial stem cells via an electrically responsive Au nanowire injector.

Biomaterials 2018 Dec 10;193:22-29. Epub 2018 Dec 10.

Department of Animal Life Science, Kangwon National University, Chuncheon, 24341, South Korea; Department of Applied Animal Science, Kangwon National University, Chuncheon, 24341, South Korea. Electronic address:

Transgenic pigs are quite useful in many biomedical fields, such as xenotransplantation research and the production of biopharmaceutical materials. The genetic transformation of porcine spermatogonial stem cells (pSSCs) followed by differentiation into mature spermatozoa enables the effective production of transgenic pigs. Improving the transfection efficiency of pSSCs, however, has been much desired. Read More

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December 2018

Draft genome sequence of human-associated streptogramin resistant Staphylococcus aureus.

J Glob Antimicrob Resist 2018 Dec 12. Epub 2018 Dec 12.

Bacterial Epidemiology and Antimicrobial Resistance Research Unit, USDA-ARS, Athens, Georgia, USA. Electronic address:

Objectives: Staphylococcus aureus is one of the leading causes of nosocomial and community-acquired infections. Treatment of those infections with macrolide-lincosamide-streptogramin (MLS) antibiotics has led to resistance to those antibiotics through various mechanisms. S. Read More

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December 2018
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Epidemiology of Antibiotic Resistance in Lebanese Extra-Hospital Settings: an overview.

J Glob Antimicrob Resist 2018 Dec 12. Epub 2018 Dec 12.

Laboratoire Microbiologie Santé et Environnement (LMSE), Ecole Doctorale des Sciences et de Technologie, Faculté de Santé Publique, Université Libanaise, Tripoli, Lebanon. Electronic address:

Antibiotics can truly be considered the symbol of the 20th century's miracle drugs. However, excessive and inappropriate use of antibiotics in human and veterinary medicine has led to repercussions in terms of mortality, morbidity, and financial and societal costs, especially with the lack of novel drugs to combat infections caused by resistant pathogens. Unfortunately, antimicrobial resistance (AMR) has increased significantly worldwide in the past decades, particularly in developing third-world countries. Read More

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December 2018

A convenient strategy to overcome interference in LC-MS/MS analysis: Application in a microdose absolute bioavailability study.

J Pharm Biomed Anal 2018 Dec 10;165:198-206. Epub 2018 Dec 10.

Bioanalytical Sciences, Bristol-Myers Squibb, Princeton, NJ, 08543, USA.

Stable isotope labeled (SIL) compounds have been commonly used as internal standards (IS) to ensure the accuracy and quality of liquid chromatography-mass spectrometry (LC-MS) bioanalytical assays. Recently, the application of SIL drugs and LC-MS assays to microdose absolute bioavailability (BA) studies has gained increasing attention. This approach can provide significant cost and time saving, and higher data quality compared to the accelerator mass spectrometry (AMS)-based method, since it avoids the use of radioactive drug, high-cost AMS instrumentation and complex measurement processes. Read More

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December 2018
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Two-dimensional porphyrin-based covalent organic framework: A novel platform for sensitive epidermal growth factor receptor and living cancer cell detection.

Biosens Bioelectron 2018 Dec 7;126:734-742. Epub 2018 Dec 7.

College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450000, PR China. Electronic address:

A porphyrin-based covalent organic framework (denoted as p-COF) was synthesized by a simple oil-bath method and exploited as a novel sensing layer for immobilizing epidermal growth factor receptor (EGFR)-targeting aptamer strands to detect trace EGFR and living michigan cancer foundation-7 (MCF-7) cells for the first time. p-COF presented a nanosheet-like structure, large cavities, rich nitrogen-bearing groups, high electrochemical activity, excellent bioaffinity, low toxicity, and good stability in aqueous solution; the microstructural features of this material enabled strong immobilization of the aptamer strands. Interactions between the aptamer strands and EGFR significantly changed the electrochemical signals of the modified electrode due to the formation of an aptamer-EGFR complex. Read More

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December 2018

Harmonization of pipeline for preclinical multicenter plasma protein and miRNA biomarker discovery in a rat model of post-traumatic epileptogenesis.

Epilepsy Res 2018 Nov 26;149:92-101. Epub 2018 Nov 26.

A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland.

The Epilepsy Bioinformatics Study for Antiepileptogenic Therapy (EpiBioS4Rx) is an international, multicenter, multidisciplinary study aimed at preventing epileptogenesis (EpiBioS4Rx: https://epibios.loni.usc. Read More

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November 2018
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Establishing a decision-support system for eco-design of biological wastewater treatment: A case study of bioaugmented constructed wetland.

Bioresour Technol 2018 Dec 7;274:425-429. Epub 2018 Dec 7.

Section of Sanitary Engineering, Department of Water Management, Delft University of Technology, Delft 2628CN, The Netherlands; Veolia Water Technologies Techno Center Netherlands B.V., Tanthofdreef 21, 2623 EW Delft, The Netherlands.

Deep treatment is a common approach to enhance pollutant removal for biological wastewater treatment technologies (BWTTs), and life cycle assessment (LCA) holds substantial advantages to support process optimization. However, there lacks of LCA-based benchmarks that cover human-nature nexuses and stakeholder involvement, which limits the guidance and eco-design of BWTTs. This study proposed a decision-support system (DSS) by linking LCA with Water Quality Model and Conjoint Analysis. Read More

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December 2018
2 Reads

Design of a randomized controlled trial on the efficacy of a reproductive health survivorship care plan in young breast cancer survivors.

Contemp Clin Trials 2018 Dec 12. Epub 2018 Dec 12.

Moores Cancer Center, University of California, San Diego, 3855 Health Sciences Dr. La Jolla, CA 92093-090, USA; Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Diego, 9300 Campus Pointe Drive, MC 7433, La Jolla, CA 92037, USA. Electronic address:

Background: Young breast cancer survivors (YBCS) have unmet needs for managing hot flashes, fertility-related concerns, sexual health and contraception.

Purpose: Describe the design and participant characteristics of a randomized controlled trial testing the efficacy of the survivorship care plan on reproductive health (SCP-R) intervention on improving hot flashes, fertility-related concerns, sexual health, and contraception in YBCS.

Methods: SCP-R is a web-based intervention with text message support encompassing evidence- based practices on four reproductive health issues. Read More

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December 2018

Triphenyltin(IV) acylhydrazone compounds: Synthesis, structure and bioactivity.

J Inorg Biochem 2018 Nov 24;191:194-202. Epub 2018 Nov 24.

Institute of Anticancer Agents Development and Theranostic Application, The Key Laboratory of Life-Organic Analysis and Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, School of Chemistry and Chemical Engineering, Qufu Normal University, Qufu 273165, China. Electronic address:

Four new triphenyltin(IV) acylhydrazone compounds of the type PhSnCHCHCONHN=R (where Ph = phenyl; R = isopropyl, isobutyl, cyclopentyl and cyclooctyl) were synthesized and characterized by elemental analysis, infrared spectrum (IR), nuclear magnetic resonance spectrum (NMR) and mass spectrum (MS). The crystal structures were determined and showed that tin atoms were four-coordinated and adopted a pseudo-tetrahedron configuration. Tin(IV) compounds show excellent bovine serum albumin (BSA) binding properties, and can oxidize nicotinamide-adenine dinucleotid (NADH) to generate reactive oxygen species (ROS), which inducing apoptosis effectively. Read More

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November 2018

Parent discipline in Thailand: Corporal punishment use and associations with myths and psychological outcomes.

Child Abuse Negl 2018 Dec 12;88:298-306. Epub 2018 Dec 12.

School of Psychology, The University of Queensland, Queensland, 4072, Australia; Institute for Teaching and Learning Innovation, The University of Queensland, Queensland, 4072, Australia. Electronic address:

A basic human right of all children is protection from physical punishment in all settings. Yet, corporal punishment remains common place within families, at home, at school, and elsewhere. In Thailand, cultural beliefs and values might preserve its use. Read More

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December 2018

Synthesis and characterization of nanostructured lipid-poloxamer organogels for enhanced skin local anesthesia.

Eur J Pharm Sci 2018 Dec 12. Epub 2018 Dec 12.

Human and Natural Sciences Center, Federal University of ABC, Santo André, SP, Brazil. Electronic address:

The aim of this study was to synthesize a novel drug delivery system using organogels (ORGs) and characterize its physicochemical properties, in vitro and ex vivo permeation abilities, cytotoxicity and in vivo local anesthetic effects. The ORG formulations contained a mixture of oleic acid-lanolin (OA-LAN), poloxamer (PL407), and the commonly used local anesthetic lidocaine (LDC). The main focus was to evaluate the impact of LAN and PL407 concentrations on the ORG structural features and their biopharmaceutical performance. Read More

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December 2018
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Anti-inflammatory action of HO-1/CO in human bronchial epithelium in response to cationic polypeptide challenge.

Mol Immunol 2018 Dec 12;105:205-212. Epub 2018 Dec 12.

School of Biomedical Sciences, The Chinese University of Hong Kong, China. Electronic address:

Carbon monoxide (CO) is an anti-inflammatory gaseous molecule produced endogenously by heme oxygenases (HOs) HO-1 and HO-2. However, the mechanisms underlying the anti-inflammatory effects of CO in the human bronchial epithelium are still not fully understood. In this study, the cationic peptide poly-l-arginine (PLA) was utilized to induce bronchial epithelial damage and subsequent pro-inflammatory cytokine release in the human bronchial epithelial cell line 16HBE14o-. Read More

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December 2018

Automatic labeling of cortical sulci for the human fetal brain based on spatio-temporal information of gyrification.

Neuroimage 2018 Dec 12. Epub 2018 Dec 12.

Fetal Neonatal Neuroimaging and Developmental Science Center, Boston Children's Hospital, Harvard Medical School, Boston, MA, 02115, USA; Division of Newborn Medicine, Boston Children's Hospital, Harvard Medical School, Boston, MA, 02115, USA. Electronic address:

Accurate parcellation and labeling of primary cortical sulci in the human fetal brain is useful for regional analysis of brain development. However, human fetal brains show large spatio-temporal changes in brain size, cortical folding patterns, and relative position/size of cortical regions, making accurate automatic sulcal labeling challenging. Here, we introduce a novel sulcal labeling method for the fetal brain using spatio-temporal gyrification information from multiple fetal templates. Read More

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December 2018

Variety matters: Diverse functions of monocyte subtypes in vascular inflammation and atherogenesis.

Vascul Pharmacol 2018 Dec 12. Epub 2018 Dec 12.

Cardiology and Angiology, Philipps-University Marburg, Marburg, Germany. Electronic address:

Monocytes are important mediators of the innate immunity by recognizing and attacking especially bacterial pathogens but also play crucial roles in various inflammatory diseases, including vascular inflammation and atherosclerosis. Maturation, differentiation and function of monocytes have been intensively explored for a long time in innumerable experimental and clinical studies. Monocytes do not represent a uniform cell type but could be further subdivided into subpopulations with distinct features and functions. Read More

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December 2018

Glucocorticoid receptor (NR3C1) gene polymorphisms are associated with age and blood parameters in Polish Caucasian nonagenarians and centenarians.

Exp Gerontol 2018 Dec 12. Epub 2018 Dec 12.

Mossakowski Medical Research Centre, PAS, Pawinskiego 5, 02-106 Warsaw, Poland; Medical Centre of Postgraduate Education, Marymoncka 99/103, 01-813 Warsaw, Poland. Electronic address:

Introduction: Polymorphism of the glucocorticoid receptor gene (NR3C1) may modify protein abundance or function and therefore disturb human homeostasis.

Methods: Variant frequencies of the three NR3C1 polymorphisms, rs2963154, rs10515522 and rs2918418, selected in silico as associated with longevity, was analyzed in 552 DNA samples from 95 to 106-year-old individuals and in 284 samples of cord blood DNA from newborns.

Results: Frequencies of the TT genotypes of rs2963154 and rs10515522, and of the rs291841 CC genotype, were higher in the long-lived study subjects (p = 0. Read More

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December 2018

Alpha-synuclein is highly prone to distribution in the hippocampus and midbrain in tree shrews, and its fibrils seed Lewy body-like pathology in primary neurons.

Exp Gerontol 2018 Dec 12. Epub 2018 Dec 12.

Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118, China; Medical Primate Research Center & Neuroscience Center, Chinese Academy of Medical Sciences, Beijing 100005, China; Yunnan Key Laboratory of Vaccine Research Development on Severe Infectious Diseases, Kunming 650118, China. Electronic address:

The Chinese tree shrew (TS) has many unique advantages that make it suitable for use as an experimental animal model for human disease including moderate body size, low cost of feeding, short reproductive cycle and lifespan, and close phylogenetic relationship to primates. Our previous studies have shown that TS treated with the mitochondrial inhibitor MPTP displayed classic Parkinsonian symptoms. Additionally, the structure of TS alpha-synuclein (α-syn) is highly homologous to that found in humans. Read More

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December 2018

Effects of acute psychosocial stress on the neural correlates of episodic encoding: Item versus associative memory.

Neurobiol Learn Mem 2018 Dec 12. Epub 2018 Dec 12.

Saarland University.

Acute stress is known to modulate episodic memory, but little is known about the extent to, and the circumstances under, which stress affects encoding of item vs. inter-item associative information for words of different valences. Furthermore, the precise neuro-cognitive mechanisms underlying stress effects on episodic encoding in humans are largely unknown. Read More

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December 2018

Revival of a Potent Therapeutic Maytansinoid Agent using a Strategy that Combines Covalent Drug Conjugation with Sequential Nanoparticle Assembly.

Int J Pharm 2018 Dec 12. Epub 2018 Dec 12.

The First Affiliated Hospital, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Key Laboratory of Combined Multi-Organ Transplantation, Ministry of Public Health, School of Medicine, Zhejiang University, Hangzhou, 310003, PR China. Electronic address:

Maytansine and its related analogues are a class of highly potent anti-proliferation agents that have failed to be exploited as clinical drugs for human therapy due to unacceptable systemic toxicity. Here, we delineate a novel strategy that combines rational drug conjugation with subsequent nanoparticle assembly to systemically deliver this highly potent and toxic drug. To demonstrate this concept, we covalently coupled the thiolated maytansine derivative, the DM1 agent, to amphiphilic block co-polymers, polyethylene glycol (PEG)-block-polylactide (PLA), in varying molecular weights to generate two prodrug constructs (i. Read More

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December 2018
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P-glycoprotein (MDR1/ABCB1) and Breast Cancer Resistance Protein (BCRP/ABCG2) limit brain accumulation of the FLT3 inhibitor quizartinib in mice.

Int J Pharm 2018 Dec 12. Epub 2018 Dec 12.

The Netherlands Cancer Institute, Division of Pharmacology, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands. Electronic address:

Quizartinib, a second-generation FLT3 inhibitor, is in clinical development for the treatment of acute myeloid leukemia. We studied its pharmacokinetic interactions with the multidrug efflux transporters ABCB1 and ABCG2 and the multidrug metabolizing enzyme CYP3A, using in vitro transport assays and knockout and transgenic mouse models. Quizartinib was transported by human ABCB1 in vitro, and by mouse (m)Abcb1 and mAbcg2 in vivo. Read More

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December 2018

Human meibum chain branching variability with age, gender and meibomian gland dysfunction.

Ocul Surf 2018 Dec 12. Epub 2018 Dec 12.

Department of Ophthalmology and Visual Sciences, University of Louisville, Louisville, KY, 40202, USA.

NMR spectroscopy was used to measure hydrocarbon chain branching variability in meibum with age, gender and Meibomian gland dysfuction (MGD). A cohort of 65 meibum donors without dry eye and 31 donors with MGD was studied. Heteronuclear 2D NMR was used to confirm CH resonance assignments allowing us to positively identify 23 of the 27 proton resonances of cholesteryl ester and H resonances due to straight chain, iso-branched and anteiso-branched hydrocarbons. Read More

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December 2018

Defective repair capacity of variant proteins of the DNA glycosylase NTHL1 for 5-hydroxyuracil, an oxidation product of cytosine.

Free Radic Biol Med 2018 Dec 12. Epub 2018 Dec 12.

Department of Tumor Pathology, Hamamatsu University School of Medicine, Hamamatsu, Japan.

The NTHL1 gene encodes DNA glycosylase, which is involved in base excision repair, and biallelic mutations of this gene result in NTHL1-associated polyposis (NAP), a hereditary disease characterized by colorectal polyposis and multiple types of carcinomas. However, no proper functional characterization of variant NTHL1 proteins has been done so far. Herein, we report functional evaluation of variant NTHL1 proteins to aid in the accurate diagnosis of NAP. Read More

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December 2018

Detection of engraftment of donor-derived antibody producing cells in a lung transplant recipient by anti-cytomegalovirus IgG avidity test.

Transpl Immunol 2018 Dec 12. Epub 2018 Dec 12.

Department of Microbiology, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima, Fukushima 960-1295, Japan.

Transplant recipients become immunocompromised through the use of immunosuppressive therapy to prevent allograft rejection. These recipients readily experience human cytomegalovirus (CMV) infection or reactivation. Therefore, CMV represents a life-threatening pathogen in transplant recipients. Read More

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December 2018

Novel predators and anthropogenic disturbance influence spatio-temporal distribution of forest antelope species.

Behav Processes 2018 Dec 12. Epub 2018 Dec 12.

School of Life Sciences, University of KwaZulu-Natal, Private Bag X01, Scottsville, Pietermaritzburg 3209, South Africa. Electronic address:

Understanding the effects of anthropogenic disturbance on species' behaviour is crucial for conservation planning, considering the extent of habitat loss. We investigated the influence of anthropogenic disturbances including agriculture, urbanisation, protected areas, and the presence of novel predators, on the temporal and spatial behaviour of sympatric forest antelope (Tragelaphus scriptus, Philantomba monticola, Sylvicapra grimmia, and Cephalophus natalensis) in an anthropogenic matrix containing forest fragments in the Indian Ocean Coastal Belt of South Africa. We integrated land-use types with camera-trap data and compared activity patterns using circular statistics and occupancy modelling. Read More

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December 2018

Effects of cellular differentiation in human primary bronchial epithelial cells: Metabolism of 4-(methylnitrosamine)-1-(3-pyridyl)-1-butanone.

Toxicol In Vitro 2018 Dec 12. Epub 2018 Dec 12.

Division of Genetic and Molecular Toxicology, Division of Biochemical Toxicology, National Center for Toxicological Research, U.S Food and Drug Administration, Jefferson, AR 72079, United States. Electronic address:

Many of the toxicants in tobacco smoke undergo biotransformation in the lungs of smokers, both to reactive and to detoxified derivatives. Human air-liquid-interface (ALI) airway tissue models have emerged as an advanced in vitro model for evaluating the toxicity of inhaled substances; however, the metabolic potential of these cultures has not been evaluated extensively. In this study, we compared the metabolic activities of an ALI tissue model to the undifferentiated normal human primary bronchial epithelial (NHBE) cells from which it was derived. Read More

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December 2018
2 Reads

The RNA helicase DHX33 is required for cancer cell proliferation in human glioblastoma and confers resistance to PI3K/mTOR inhibition.

Cell Signal 2018 Dec 12. Epub 2018 Dec 12.

Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong, China. Electronic address:

Human Glioblastoma is one deadly disease; the median survival time is reported to be 13.9 months after treatment. In the present study, we discovered that DHX33 is highly expressed in 84% of all Glioblastoma multiforme (GBM). Read More

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December 2018

A potential regulatory network among WDR86-AS1, miR-10b-3p, and LITAF is possibly involved in preeclampsia pathogenesis.

Cell Signal 2018 Dec 12. Epub 2018 Dec 12.

Department of Obstetrics and Gynecology, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, PR China.

Preeclampsia (PE), a pregnancy-specific disorder, is a leading cause of perinatal maternal and fetal mortality and morbidity. Impaired migration and invasion of trophoblastic cells and an imbalanced systemic maternal inflammatory response have been proposed as possible causes of pathogenesis of PE. Comparative analysis of PE-affected placentas and healthy placentas has uncovered differentially expressed long noncoding RNAs, microRNAs, and mRNAs. Read More

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December 2018

FH535 inhibits proliferation and migration of colorectal cancer cells by regulating CyclinA2 and Claudin1 gene expression.

Gene 2018 Dec 12. Epub 2018 Dec 12.

Wenzhou Medical University School of Laboratory Medicine and Life Sciences, Wenzhou 325000, China. Electronic address:

Wnt signaling pathway plays a major role in the progression of colorectal cancer (CRC). Small molecules which can cut off this signal transduction can be promising anti-cancer drugs for CRC therapy. Therefore, we aimed to investigate the mechanisms of FH535, an inhibitor of the Wnt signaling pathway, on inhibiting proliferation and migration of colorectal cancer cells DLD-1 and SW620. Read More

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December 2018
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Characterization of HPV integration, viral gene expression and E6E7 alternative transcripts by RNA-Seq: A descriptive study in invasive cervical cancer.

Genomics 2018 Dec 12. Epub 2018 Dec 12.

Genetics Program, Instituto Nacional de Câncer (INCA), Rio de Janeiro, Brazil. Electronic address:

Scarce data are available on the expression of Papillomavirus genome and the frequency of alternatively spliced E6/E7 mRNAs in invasive cervical cancer. We carried out a comprehensive characterization of HPV expression by RNA-Seq analysis in 22 invasive cervical cancer with HPV16 or HPV18, characterizing the presence of integrated/episomal viral DNA, the integration sites in human genome and the proportion of alternative splicing products of E6 and E7 genes. The expression patterns suggested the presence of episomal and/or integrated viral DNA, with integration detected in most tumors, frequently occurring within human genes in HPV18+ and in intergenic regions in HPV16+ tumors. Read More

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December 2018

Ultra-high field magnetic resonance imaging parameter mapping in the posterior horn of ex vivo human menisci.

Osteoarthritis Cartilage 2018 Dec 12. Epub 2018 Dec 12.

Medical Radiation Physics, Department of Translational Medicine, Lund University, Malmö, Sweden; Medical Imaging and Physiology, Skåne University Hospital, Lund, Sweden. Electronic address:

Objective: To investigate the relationship between meniscus magnetic resonance (MR) relaxation parameters and meniscus degradation through quantitative imaging of ex vivo posterior horns of menisci from subjects with and without knee osteoarthritis (OA).

Design: We sampled medial and lateral menisci from ten medial compartment knee OA patients (mean age 63 years) undergoing total knee replacement and from ten deceased donors (references, mean age 51 years). MR relaxation parameters T2*, T2 and T1 of the posterior horn were measured at a 9. Read More

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December 2018

Osteoarthritis Year in Review 2018: biomarkers (biochemical markers).

Osteoarthritis Cartilage 2018 Dec 12. Epub 2018 Dec 12.

Biomarker and research, Nordic Bioscience, Herlev, Denmark. Electronic address:

Objective: The aim of this narrative review is to summarize important findings from biochemical marker studies relevant to osteoarthritis (OA) in the context of new discoveries and clinical and scientific need.

Design: We conducted a systematic search of electronic medical databases (Embase, Medline, Web of Science, Cochrane central) between 01-03-2017 and 31-03-2018. The search was restricted to human studies, English language and full text available publications while reviews were excluded. Read More

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December 2018

Protocol for evaluation of topical ophthalmic drug products in different compartments of fresh eye tissues in a rabbit model.

J Pharmacol Toxicol Methods 2018 Dec 12. Epub 2018 Dec 12.

Office of Translational Science, Office of Clinical Pharmacology, Division of Applied Regulatory Science, U.S. Food and Drug Administration, Center for Drug Evaluation and Research, White Oak Federal Research Center, 10903 New Hampshire Ave, Silver Spring, MD 20993, USA. Electronic address:

Topical ophthalmic drugs are the most commonly used dosage form to treat diseases of the anterior segment of the eye. Although this dosage form has the advantages of ease of application, small volume dose, and rapid action and is largely devoid of systemic adverse effects, the bioavailability is low due to pre-corneal anatomical barriers and the nature of the drug formulation itself. Some complex generic formulations (suspensions, ointments, gels) for topical ophthalmic products face impediments to rapid regulatory approval because of the complex nature of the formulations and difficulties in determining bioequivalence with the innovator product. Read More

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December 2018

Effects of sex and menstrual cycle phase on cardiac response and alpha- amylase levels in psychosocial stress.

Biol Psychol 2018 Dec 12. Epub 2018 Dec 12.

Laboratory of Social Cognitive Neuroscience, IDOCAL, Department of Psychobiology, University of Valencia, 46010 Valencia, Spain.

The impact of sex and the menstrual cycle phase on the autonomic response to psychosocial stress remains controversial. This study explored autonomic nervous system activity through salivary alpha-amylase, heart rate, and heart rate variability responses to the Trier Social Stress Test (TSST) in healthy young people. The sample was composed of 25 men, 26 women in the luteal phase, and 25 women in the follicular phase, from 18 to 25 years of age. Read More

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December 2018

The testosterone metabolite 3α-androstanediol inhibits oxidative stress-induced ERK phosphorylation and neurotoxicity in SH-SY5Y cells through an MKP3/DUSP6-dependent mechanism.

Neurosci Lett 2018 Dec 12. Epub 2018 Dec 12.

Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G 2W1, Canada. Electronic address:

Testosterone exerts neuroprotective effects on the brain, but the mechanisms by which these effects are exerted appear to be different in males and females. While in females they involve local conversion to estradiol, in males they may be androgen receptor-dependent, or mediated through metabolism to neurosteroids such as 5α-androstane-3α,17β-diol (3α-diol), which acts through different mechanisms than testosterone itself. Recently, we demonstrated that 3α-diol can protect neurons and neuronal-like cells against oxidative stress-induced neurotoxicity associated with prolonged phosphorylation of the extracellular signal-regulated kinase (ERK). Read More

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December 2018

Human olfactory stem cells: As a promising source of dopaminergic neuron-like cells for treatment of Parkinson's disease.

Neurosci Lett 2018 Dec 12. Epub 2018 Dec 12.

Applied Biophotonics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran. Electronic address:

The production of dopaminergic (DA) neurons from stem cells holds a great promise for future clinical treatment of neurodegenerative diseases, such as Parkinson's disease (PD). Olfactory ecto-mesenchymal stem cells (OE-MSCs) derived from the adult human olfactory mucosa can be easily isolated and expanded in culture while maintaining their immense plasticity. In addition to reduced ethical concerns, OE-MSCs could be transplanted across allogeneic barriers, making them desirable stem cells for clinical applications. Read More

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December 2018

Alternative splicing of the vitamin D receptor modulates target gene expression and promotes ligand-independent functions.

Toxicol Appl Pharmacol 2018 Dec 12. Epub 2018 Dec 12.

Department of Environmental and Molecular Toxicology, Oregon State University, 1007 Agriculture & Life Sciences Building, Corvallis, OR 97331; USA; LS Pharma, 884 Park St., Lebanon, OR 97355; USA.

Alternative splicing modulates gene function by creating splice variants with alternate functions or non-coding RNA activity. Naturally occurring variants of nuclear receptor (NR) genes with dominant negative or gain-of-function phenotypes have been documented, but their cellular roles, regulation, and responsiveness to environmental stress or disease remain unevaluated. Informed by observations that class I androgen and estrogen receptor variants display ligand-independent signaling in human cancer tissues, we questioned whether the function of class II NRs, like the vitamin D receptor (VDR), would also respond to alternative splicing regulation. Read More

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December 2018
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