Direct detection of Anaplasma phagocytophilum by polymerase chain reaction followed by electrospray ionization mass spectrometry from human blood.
- Heimo Lagler,
- Nicole Harrison,
- Manuel Kussmann,
- Markus Obermüller,
- Heinz Burgmann,
- Athanasios Makristathis,
- Michael Ramharter
Int J Infect Dis 2017 May 16. Epub 2017 May 16.
Department of Medicine 1, Division of Infectious Diseases and Tropical Medicine, Medical University of Vienna, Vienna, Austria; Institut für Tropenmedizin, Eberhard Karls Universität Tübingen, Tübingen, Germany. Electronic address:
Bacterial pathogens not detectable in commercial blood culture assays constitute an important challenge for infectious disease physicians, in particular if clinical symptoms of the illness are unspecific. In this report Anaplasma phagocytophilum was detected directly from a peripheral blood sample of a febrile patient reporting a tick bite by a commercial system based on polymerase chain reaction followed by electrospray ionization mass spectrometry. Therefore, the diagnosis of human granulocytic anaplasmosis infection was established by this diagnostic methodology for the first time. Read More