2,922 results match your criteria Histochemistry and cell biology[Journal]


Quantitative pixel intensity- and color-based image analysis on minimally compressed files: implications for whole-slide imaging.

Histochem Cell Biol 2019 Apr 13. Epub 2019 Apr 13.

Australian Center for Microscopy and Microanalysis, The University of Sydney, Sydney, NSW, 2006, Australia.

Current best practice in the quantitative analysis of microscopy images dictates that image files should be saved in a lossless format such as TIFF. Use of lossy files, including those processed with the JPEG algorithm, is highly discouraged due to effects of compression on pixel characteristics. However, with the growing popularity of whole-slide imaging (WSI) and its attendant large file sizes, compressed image files are becoming more prevelent. Read More

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http://dx.doi.org/10.1007/s00418-019-01783-7DOI Listing

A temporal and spatial map of axons in developing mouse prostate.

Histochem Cell Biol 2019 Apr 11. Epub 2019 Apr 11.

Molecular and Environmental Toxicology Center, University of Wisconsin-Madison, Madison, WI, USA.

Prostate autonomic and sensory axons control glandular growth, fluid secretion, and smooth muscle contraction and are remodeled during cancer and inflammation. Morphogenetic signaling pathways reawakened during disease progression may drive this axon remodeling. These pathways are linked to proliferative activities in prostate cancer and benign prostate hyperplasia. Read More

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http://link.springer.com/10.1007/s00418-019-01784-6
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http://dx.doi.org/10.1007/s00418-019-01784-6DOI Listing
April 2019
3 Reads

Expression and localization of diacylglycerol kinase ζ in guinea pig cochlea and its functional implication under noise-exposure stress conditions.

Histochem Cell Biol 2019 Apr 8. Epub 2019 Apr 8.

Department of Otolaryngology, Head and Neck Surgery, Yamagata University Faculty of Medicine, 2-2-2 Iida-Nishi, Yamagata-shi, Yamagata, 990-9585, Japan.

Cochlear hair cells are essential for the mechanotransduction of hearing. Sensorineural hearing loss can be irreversible because hair cells have a minimal ability to repair or regenerate themselves once damaged. In order to develop therapeutic interventions to prevent hair cell loss, it is necessary to understand the signaling pathway operating in cochlear hair cells and its alteration upon damage. Read More

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http://dx.doi.org/10.1007/s00418-019-01781-9DOI Listing

Interleukin-6 contributes to initiation of neuronal regeneration program in the remote dorsal root ganglia neurons after sciatic nerve injury.

Histochem Cell Biol 2019 Mar 29. Epub 2019 Mar 29.

Department of Anatomy, Cellular and Molecular Research Group, Faculty of Medicine, Masaryk University, Kamenice 3, 62500, Brno, Czech Republic.

To assess the potential role of IL-6 in sciatic nerve injury-induced activation of a pro-regenerative state in remote dorsal root ganglia (DRG) neurons, we compared protein levels of SCG-10 and activated STAT3, as well as axon regeneration in IL-6 knockout (IL-6ko) mice and their wild-type (WT) counterparts. Unilateral sciatic nerve compression and transection upregulated SCG-10 protein levels and activated STAT3 in DRG neurons not only in lumbar but also in cervical segments of WT mice. A pro-regenerative state induced by prior sciatic nerve lesion in cervical DRG neurons of WT mice was also shown by testing for axon regeneration in crushed ulnar nerve. Read More

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http://dx.doi.org/10.1007/s00418-019-01779-3DOI Listing

Similarities and differences in tissue distribution of DLK1 and DLK2 during E16.5 mouse embryogenesis.

Histochem Cell Biol 2019 Mar 19. Epub 2019 Mar 19.

Department of Inorganic and Organic Chemistry and Biochemistry, Medical School, Regional Center for Biomedical Research, Unidad Asociada de Biomedicina (UCLM-CSIC), University of Castilla-La Mancha, Calle Almansa 14, 02008, Albacete, Spain.

DLK1 and DLK2 are transmembrane proteins belonging to the EGF-like repeat-containing family that function as non-canonical NOTCH inhibitory ligands. DLK1 is usually downregulated after embryo development and its distribution in some adult and embryonic tissues has been described. However, the expression and role of DLK2 in embryo and adult tissues remains unclear. Read More

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http://dx.doi.org/10.1007/s00418-019-01778-4DOI Listing

In focus in HCB.

Histochem Cell Biol 2019 Apr 16;151(4):279-281. Epub 2019 Mar 16.

University of Zurich, 8091, Zurich, Switzerland.

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http://dx.doi.org/10.1007/s00418-019-01777-5DOI Listing
April 2019
1 Read

Improved immunohistochemical detection of phosphorylated mitogen-activated protein kinases in the injured rat optic nerve head.

Histochem Cell Biol 2019 Mar 11. Epub 2019 Mar 11.

Ophthalmic Research Laboratories, Central Adelaide Local Health Network, Level 7 Adelaide Health & Medical Sciences Building, University of Adelaide, Adelaide, SA, Australia.

The activity of mitogen-activated protein kinases (MAPKs) is largely controlled by addition or removal of phosphate groups, which are carried out by kinase or phosphatase enzymes, respectively. Determining the phosphorylation status of MAPK isoenzymes, therefore, aids elucidation of the physiological and pathological roles of this enzyme. In practical terms, however, end-point procurement of appropriate experimental tissues produces conditions where MAPK phosphorylation status can rapidly alter, thus giving rise to aberrant data. Read More

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http://dx.doi.org/10.1007/s00418-019-01771-xDOI Listing
March 2019
4 Reads

In focus in HCB.

Histochem Cell Biol 2019 Mar 19;151(3):199-200. Epub 2019 Feb 19.

University of Zurich, 8091, Zurich, Switzerland.

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http://link.springer.com/10.1007/s00418-019-01773-9
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http://dx.doi.org/10.1007/s00418-019-01773-9DOI Listing
March 2019
6 Reads

High-throughput quantification of the effect of DMSO on the viability of lung and breast cancer cells using an easy-to-use spectrophotometric trypan blue-based assay.

Histochem Cell Biol 2019 Feb 16. Epub 2019 Feb 16.

Sharjah Institute for Medical Research, College of Medicine, University of Sharjah, 27272, Sharjah, UAE.

One of the main aspects investigated in potential therapeutic compounds is their effect on cells viability and proliferative ability. Although various methods have been developed to investigate these aspects, these methods present with shortcomings in terms of either cost, availability, accuracy, precision, or throughput. This study describes a simple, economic, reproducible, and high-throughput assay to quantify cell death and proliferation. Read More

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http://link.springer.com/10.1007/s00418-019-01775-7
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http://dx.doi.org/10.1007/s00418-019-01775-7DOI Listing
February 2019
3 Reads

Fluorescence microscope light source stability.

Histochem Cell Biol 2019 Apr 14;151(4):357-366. Epub 2019 Feb 14.

Department of Physiology, McGill University, Montreal, Canada.

The process of fluorescence starts with the efficient generation of light that is required for the excitation of fluorophores. As such, light sources are a crucial component of a fluorescence microscope. Choosing the right illumination tool can not only improve the quality of experimental results, but also the microscope's economic and environmental footprint. Read More

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http://link.springer.com/10.1007/s00418-019-01776-6
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http://dx.doi.org/10.1007/s00418-019-01776-6DOI Listing
April 2019
7 Reads

Regulatory effects of PRF and titanium surfaces on cellular adhesion, spread, and cytokine expressions of gingival keratinocytes.

Histochem Cell Biol 2019 Feb 14. Epub 2019 Feb 14.

Department of Periodontology, Institute of Dentistry, University of Turku, Lemminkäisenkatu 2, 20520, Turku, Finland.

Dental implant material has an impact on adhesion and spreading of oral mucosal cells on its surface. Platelet-rich fibrin (PRF), a second-generation platelet concentrate, can enhance cell proliferation and adhesion. The aim was to examine the regulatory effects of PRF and titanium surfaces on cellular adhesion, spread, and cytokine expressions of gingival keratinocytes. Read More

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http://link.springer.com/10.1007/s00418-019-01774-8
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http://dx.doi.org/10.1007/s00418-019-01774-8DOI Listing
February 2019
6 Reads

Novel methods in adrenal research: a metabolomics approach.

Histochem Cell Biol 2019 Mar 6;151(3):201-216. Epub 2019 Feb 6.

Research Unit Analytical Pathology, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Neuherberg, Germany.

Metabolic alterations have implications in a spectrum of tissue functions and disease. Aided by novel molecular biological and computational tools, our understanding of physiological and pathological processes underpinning endocrine and endocrine-related disease has significantly expanded over the last decade. Herein, we focus on novel metabolomics-related methodologies in adrenal research: in situ metabolomics by mass spectrometry imaging, steroid metabolomics by gas and liquid chromatography-mass spectrometry, energy pathway metabologenomics by liquid chromatography-mass spectrometry-based metabolomics of Krebs cycle intermediates, and cellular reprogramming to generate functional steroidogenic cells and hence to modulate the steroid metabolome. Read More

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http://dx.doi.org/10.1007/s00418-019-01772-wDOI Listing
March 2019
2 Reads

Region-specific changes in brain kisspeptin receptor expression during estrogen depletion and the estrous cycle.

Histochem Cell Biol 2019 Jan 22. Epub 2019 Jan 22.

Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School, Sendagi 1-1-5, Bunkyo-ku, Tokyo, 113-8602, Japan.

Kisspeptin acts as a potent neuropeptide regulator of reproduction through modulation of the hypothalamic-pituitary-gonadal axis. Previous studies revealed sex differences in brain expression patterns as well as regulation of expression by estrogen. Alternatively, sex differences and estrogen regulation of the kisspeptin receptor (encoded by Kiss1r) have not been examined at cellular resolution. Read More

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http://dx.doi.org/10.1007/s00418-018-01767-zDOI Listing
January 2019

Characterization of blood-brain barrier integrity in a B-cell-dependent mouse model of multiple sclerosis.

Histochem Cell Biol 2019 Jan 21. Epub 2019 Jan 21.

Department of Anatomy and Cell Biology, University of Würzburg, Würzburg, Germany.

Recent studies with B-cell-depleting antibodies have demonstrated clinical success in the treatment of multiple sclerosis (MS) patients. While these antibodies efficiently target B cells in the blood, it is unclear how effective they are in the central nervous system (CNS), especially in the context of limited blood-brain barrier (BBB) permeability and the ongoing discussion on the relevance of B-cell aggregate formation in the brains of SP-MS patients. The aim of this study was to evaluate BBB integrity in the context of B-cell-dependent neuroinflammation in a mouse model of MS. Read More

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http://dx.doi.org/10.1007/s00418-019-01768-6DOI Listing
January 2019
1 Read

Osteoclasts secrete osteopontin into resorption lacunae during bone resorption.

Histochem Cell Biol 2019 Jan 14. Epub 2019 Jan 14.

Department of Anatomy and Cell Biology, Cancer Research and Translational Medicine Research Unit, Faculty of Medicine, University of Oulu, P.O. Box 5000, Aapistie 5, 90014, Oulu, Finland.

Osteopontin (OPN) is a non-collagenous extracellular sialylated glycoprotein located in bone. It is believed to be one of the key components in osteoclast attachment to bone during resorption. In this study, we characterized OPN and other glycoproteins found in the resorption lacunae to confirm the role of osteoclasts in OPN secretion using electron microscopy and mass spectrometry. Read More

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http://link.springer.com/10.1007/s00418-019-01770-y
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http://dx.doi.org/10.1007/s00418-019-01770-yDOI Listing
January 2019
16 Reads

In focus in HCB.

Histochem Cell Biol 2019 Feb 7;151(2):97-99. Epub 2019 Jan 7.

University of Zurich, 8091, Zurich, Switzerland.

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http://dx.doi.org/10.1007/s00418-019-01769-5DOI Listing
February 2019
1 Read

Alarin in different human intestinal epithelial cell types.

Histochem Cell Biol 2019 Jan 5. Epub 2019 Jan 5.

Institute of Anatomy and Cell Biology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany.

Alarin (AL), a new member of the galanin family, has been localized in various CNS regions, mainly in rodents. Among other effects, it modulates food intake. Therefore, we analyzed the immunohistochemical distribution pattern of AL in human intestinal epithelia. Read More

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http://dx.doi.org/10.1007/s00418-018-1763-9DOI Listing
January 2019
1 Read

An update on the Golgi staining technique improving cerebellar cell type specificity.

Histochem Cell Biol 2019 Apr 3;151(4):327-341. Epub 2019 Jan 3.

Institute of Anatomy, Anatomy and Cell Biology, University of Bonn, Nussallee 10, 53115, Bonn, Germany.

The detailed morphological characterization of single cells was a major breakthrough in neuroscience during the turn of the twentieth century, enabling Ramon y Cajal to postulate the neuron doctrine. Even after 150 years, single cell analysis is an intriguing goal, newly motivated by the finding that autism might be caused by intricate and discreet changes in cerebellar morphology. Besides new single labelling technologies, the Golgi staining technique is still in use due to its whole cell labelling characteristics, its superior contrast performance over other methods and its apparent randomness of staining cells within a whole tissue block. Read More

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http://dx.doi.org/10.1007/s00418-018-01766-0DOI Listing
April 2019
1 Read

Coping with formalin banning in pathology: under vacuum long-term tissue storage with no added formalin.

Histochem Cell Biol 2019 Jan 2. Epub 2019 Jan 2.

Anatomic Pathology, Department of Surgical Sciences and Integrated Diagnostics (DISC), University of Genoa, via De Toni 14, 16132, Genoa, Italy.

Formalin is toxic and has recently been classified as carcinogenic leading to a proposed European formalin ban. But, the pathology use of formalin has however been completely overlooked, and this is proving to be a relevant issue, as no alternative, reliable, tissue fixative is available. Various systems have been proposed to reduce formalin use and exposure; long-term storage and disposal of formalin is also a problem. Read More

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http://dx.doi.org/10.1007/s00418-018-1765-7DOI Listing
January 2019
1 Read

Differential expression of PKCα and -β in the zebrafish retina.

Histochem Cell Biol 2019 Jan 2. Epub 2019 Jan 2.

Institute of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland.

The retina is a complex neural circuit, which processes and transmits visual information from light perceiving photoreceptors to projecting retinal ganglion cells. Much of the computational power of the retina rests on signal integrating interneurons, such as bipolar cells. Commercially available antibodies against bovine and human conventional protein kinase C (PKC) α and -β are frequently used as markers for retinal ON-bipolar cells in different species, despite the fact that it is not known which bipolar cell subtype(s) they actually label. Read More

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http://dx.doi.org/10.1007/s00418-018-1764-8DOI Listing
January 2019
1 Read

 In focus in HCB.

Histochem Cell Biol 2019 Jan 17;151(1):1-3. Epub 2018 Dec 17.

University of Zurich, 8091, Zurich, Switzerland.

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http://link.springer.com/10.1007/s00418-018-1762-x
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http://dx.doi.org/10.1007/s00418-018-1762-xDOI Listing
January 2019
6 Reads

Histological processing of un-/cellularized thermosensitive electrospun scaffolds.

Histochem Cell Biol 2019 Apr 17;151(4):343-356. Epub 2018 Dec 17.

Department of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University of Graz, Neue Stiftingtalstraße 6, 8010, Graz, Austria.

Histological processing of thermosensitive electrospun poly(ε-caprolactone)/poly(L-lactide) (PCL/PLA) scaffolds fails, as poly(ε-caprolactone) (PCL) is characterized by its low-melting temperature (Tm = 60 °C). Here, we present an optimized low-temperature preparation method for the histological processing of un-/cellularized thermosensitive PCL/PLA scaffolds.Our study is aimed at the establishment of an optimized dehydration and low-melting-point paraffin-embedding method of electrospun PCL/PLA scaffolds (un-/cellularized). Read More

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http://link.springer.com/10.1007/s00418-018-1757-7
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http://dx.doi.org/10.1007/s00418-018-1757-7DOI Listing
April 2019
13 Reads

Correction to: P2X7R-dependent regulation of glycogen synthase kinase 3β and claudin-18 in alveolar epithelial type I cells of mice lung.

Histochem Cell Biol 2019 Mar;151(3):277

Institute of Anatomy, Medical Faculty "Carl Gustav Carus", Technical University Dresden, Fetscherstr. 74, 01307, Dresden, Germany.

Correction to: Histochem Cell Biol (2016). Read More

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http://dx.doi.org/10.1007/s00418-018-1758-6DOI Listing
March 2019
1 Read

α-Parvin and β-parvin in the rat uterus during decidualisation and uterine receptivity.

Histochem Cell Biol 2018 Dec 5. Epub 2018 Dec 5.

Cell and Reproductive Biology Lab, Discipline of Anatomy and Histology, School of Medical Sciences, University of Sydney, Camperdown, Australia.

During early pregnancy, the uterine luminal epithelial cells (UECs) and endometrial stromal cells (ESCs) undergo morphological changes to enable blastocyst implantation. The present study investigates, for the first time, the cytoskeletal-associated proteins and α-actinin superfamily members, α-parvin and β-parvin, during early pregnancy in the rat uterus. These two PARVA proteins are involved in cell adhesion, morphological changes and regulation of other cytoskeletal proteins, through binding with proteins such as actin and integrin-linked kinase. Read More

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http://dx.doi.org/10.1007/s00418-018-1761-yDOI Listing
December 2018
2 Reads

Histone deacetylase inhibitors suppress transdifferentiation of gonadotrophs to prolactin cells and proliferation of prolactin cells induced by diethylstilbestrol in male mouse pituitary.

Histochem Cell Biol 2019 Apr 3;151(4):291-303. Epub 2018 Dec 3.

Department of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.

Diethylstilbestrol (DES), an estrogen agonist, increases prolactin (PRL) cells through transdifferentiation of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) cells to PRL cells as well as proliferation of PRL cells in adult male mouse pituitary. Since hyperacetylation of histone H3 is implicated in the regulation of activation of various genes, we examined the effect of DES on the state of histone H3 acetylation. DES significantly reduced the immunohistochemical signal for acetylated histone H3 at lysine 9 (H3K9ac) in PRL, LH and FSH cells, but not for H3K18ac or H3K23ac. Read More

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http://dx.doi.org/10.1007/s00418-018-1760-zDOI Listing
April 2019
5 Reads

Detection of herpesvirus capsids in transmission electron microscopy images using transfer learning.

Histochem Cell Biol 2019 Feb 28;151(2):101-114. Epub 2018 Nov 28.

Central Facility for Electron Microscopy, Ulm University, Ulm, Germany.

The detailed analysis of secondary envelopment of the Human betaherpesvirus 5/human cytomegalovirus (HCMV) from transmission electron microscopy (TEM) images is an important step towards understanding the mechanisms underlying the formation of infectious virions. As a step towards a software-based quantification of different stages of HCMV virion morphogenesis in TEM, we developed a transfer learning approach based on convolutional neural networks (CNNs) that automatically detects HCMV nucleocapsids in TEM images. In contrast to existing image analysis techniques that require time-consuming manual definition of structural features, our method automatically learns discriminative features from raw images without the need for extensive pre-processing. Read More

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http://dx.doi.org/10.1007/s00418-018-1759-5DOI Listing
February 2019
5 Reads

Correction to: The proliferation marker pKi-67 becomes masked to MIB-1 staining after expression of its tandem repeats.

Histochem Cell Biol 2019 Mar;151(3):275

Surgical Research Laboratory, Department of Surgery, Medical University of Lübeck, Ratzeburger Allee 160, 23538, Lübeck, Germany.

In the PubMed citation records, the author's name shows. Read More

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http://dx.doi.org/10.1007/s00418-018-1755-9DOI Listing
March 2019
4 Reads

In focus in HCB.

Histochem Cell Biol 2018 Dec 16;150(6):575-578. Epub 2018 Nov 16.

University of Zurich, 8091, Zurich, Switzerland.

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http://dx.doi.org/10.1007/s00418-018-1754-xDOI Listing
December 2018
1 Read

Molecular imaging with nanoparticles: the dwarf actors revisited 10 years later.

Histochem Cell Biol 2018 Dec 16;150(6):733-794. Epub 2018 Nov 16.

Division of Histology and Embryology, Department of Anatomy, Medical University Innsbruck, Muellerstrasse 59, 6020, Innsbruck, Austria.

We explore present-day trends and challenges in nanomedicine. Creativity in the laboratories continues: the published literature on novel nanoparticles is now vast. Nanoagents are discussed here which are composed entirely of strongly photoluminescent materials, tunable to desired optical properties and of inherently low toxicity. Read More

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http://link.springer.com/10.1007/s00418-018-1753-y
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http://dx.doi.org/10.1007/s00418-018-1753-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6267421PMC
December 2018
24 Reads

Wound healing mechanism in Mongolian gerbil skin.

Histochem Cell Biol 2019 Mar 3;151(3):229-238. Epub 2018 Nov 3.

Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemoon-Gu, Seoul, 03722, South Korea.

The skin wound healing ability of animals differs depending on the environment. The gerbil wound model showed a different wound healing mechanism than was known thus far. Many other wound healing mechanisms have been found to involve transforming growth factor-beta 1 (TGF-β1). Read More

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http://link.springer.com/10.1007/s00418-018-1752-z
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http://dx.doi.org/10.1007/s00418-018-1752-zDOI Listing
March 2019
13 Reads

The micromechanics of lung alveoli: structure and function of surfactant and tissue components.

Histochem Cell Biol 2018 Dec 2;150(6):661-676. Epub 2018 Nov 2.

Institute of Functional and Applied Anatomy, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany.

The mammalian lung´s structural design is optimized to serve its main function: gas exchange. It takes place in the alveolar region (parenchyma) where air and blood are brought in close proximity over a large surface. Air reaches the alveolar lumen via a conducting airway tree. Read More

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http://dx.doi.org/10.1007/s00418-018-1747-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6267411PMC
December 2018
2 Reads

How high resolution 3-dimensional imaging changes our understanding of postnatal lung development.

Histochem Cell Biol 2018 Dec 2;150(6):677-691. Epub 2018 Nov 2.

Institute of Anatomy, University of Bern, Baltzerstrasse 2, 3012, Bern, Switzerland.

During the last 10 + years biologically and clinically significant questions about postnatal lung development could be answered due to the application of modern cutting-edge microscopic and quantitative histological techniques. These are in particular synchrotron radiation based X-ray tomographic microscopy (SRXTM), but also Helium Magnetic Resonance Imaging, as well as the stereological estimation of the number of alveoli and the length of the free septal edge. First, the most important new finding may be the following: alveolarization of the lung does not cease after the maturation of the alveolar microvasculature but continues until young adulthood and, even more important, maybe reactivated lifelong if needed to rescue structural damages of the lungs. Read More

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http://dx.doi.org/10.1007/s00418-018-1749-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6267404PMC
December 2018
1 Read

Genes responsible for proliferation, differentiation, and junction adhesion are significantly up-regulated in human ovarian granulosa cells during a long-term primary in vitro culture.

Histochem Cell Biol 2019 Feb 31;151(2):125-143. Epub 2018 Oct 31.

Department of Anatomy, Poznan University of Medical Sciences, 6 Święcickiego St, 60-781, Poznań, Poland.

The human ovarian granulosa cells (GCs) surround the oocyte and form the proper architecture of the ovarian follicle. The ability of GCs to proliferate and differentiate in the conditions of in vitro culture has been proven. However, there is still a large field for extensive investigation of molecular basics, as well as marker genes, responsible for these processes. Read More

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http://link.springer.com/10.1007/s00418-018-1750-1
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http://dx.doi.org/10.1007/s00418-018-1750-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6394675PMC
February 2019
2 Reads

The role of autophagy in morphogenesis and stem cell maintenance.

Histochem Cell Biol 2018 Dec 31;150(6):721-732. Epub 2018 Oct 31.

Department of Anatomy and Molecular Embryology, Institute of Anatomy, Ruhr University Bochum, Universitätsstrasse 150, 44801, Bochum, Germany.

During embryonic development, cells need to undergo a number of morphological changes that are decisive for the shaping of the embryo's body, initiating organogenesis and differentiation into functional tissues. These remodeling processes are accompanied by profound changes in the cell membrane, the cytoskeleton, organelles, and extracellular matrix composition. While considerably detailed insight into the role of autophagy in stem cells biology has been gained in the recent years, information regarding the participation of autophagy in morphogenetic processes is only sparse. Read More

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http://link.springer.com/10.1007/s00418-018-1751-0
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http://dx.doi.org/10.1007/s00418-018-1751-0DOI Listing
December 2018
17 Reads

Restriction of drug transport by the tumor environment.

Histochem Cell Biol 2018 Dec 25;150(6):631-648. Epub 2018 Oct 25.

Institute of Anatomy and Cell Biology, Universität Würzburg, Koellikerstrasse 6, 97070, Würzburg, Germany.

As in the systemic treatment of any disease, it is crucial for anti-cancer drugs to reach their target at a sufficient that is a therapeutically effective dose. However, unlike normal organs, solid tumors have a tendency to be undersupplied and hypoxic. This not only leads to insufficient supply of oxygen and nutrients but also to inefficient transport of drugs into tumors. Read More

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http://dx.doi.org/10.1007/s00418-018-1744-zDOI Listing
December 2018
1 Read

Moonlighting nuclear pore proteins: tissue-specific nucleoporin function in health and disease.

Histochem Cell Biol 2018 Dec 25;150(6):593-605. Epub 2018 Oct 25.

Institute of Molecular Biology and Medicine, Université Libre de Bruxelles, Rue Profs. Jeener et Brachet 12, 6041, Gosselies, Belgium.

The nuclear pore complex is the main transportation hub for exchange between the cytoplasm and the nucleus. It is built from nucleoporins that form distinct subcomplexes to establish this huge protein complex in the nuclear envelope. Malfunctioning of nucleoporins is well known in human malignancies, such as gene fusions of NUP214 and NUP98 in hematological neoplasms and overexpression of NUP88 in a variety of human cancers. Read More

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http://link.springer.com/10.1007/s00418-018-1748-8
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http://dx.doi.org/10.1007/s00418-018-1748-8DOI Listing
December 2018
40 Reads

The membrane palmitoylated protein, MPP6, is involved in myelin formation in the mouse peripheral nervous system.

Histochem Cell Biol 2018 Oct 24. Epub 2018 Oct 24.

Health Science Division, Department of Medical Sciences, Graduate School of Medicine, Science and Technology, Shinshu University, 3-1-1 Asahi, Matsumoto City, Nagano, 390-8621, Japan.

A membrane skeletal molecular complex, protein 4.1G-membrane palmitoylated protein 6 (MPP6)-Lin7-cell adhesion molecule 4 (CADM4), is incorporated in Schwann cells, especially in Schmidt-Lanterman incisures (SLIs), in the mouse peripheral nervous system (PNS). MPP6, Lin7, and CADM4 are transported to SLIs by 4. Read More

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http://dx.doi.org/10.1007/s00418-018-1745-yDOI Listing
October 2018
2 Reads

Diversity of enteroendocrine cells investigated at cellular and subcellular levels: the need for a new classification scheme.

Histochem Cell Biol 2018 Dec 24;150(6):693-702. Epub 2018 Oct 24.

Department of Anatomy and Neuroscience, University of Melbourne, Parkville, VIC, 3010, Australia.

Enteroendocrine cells were historically classified by a letter code, each linked to a single hormone, deduced to be the only hormone produced by the cell. One type, the L cell, was recognised to store and secrete two products, peptide YY (PYY) and glucagon-related peptides. Many other exceptions to the one-cell one-hormone classifications have been reported over the last 40 years or so, and yet the one-hormone dogma has persisted. Read More

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http://dx.doi.org/10.1007/s00418-018-1746-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447040PMC
December 2018
2 Reads

Characteristics of the tissue section that influence the staining outcome in immunohistochemistry.

Histochem Cell Biol 2019 Jan 24;151(1):91-96. Epub 2018 Oct 24.

Rudbeck Laboratory, Department of Immunology, Genetics and Pathology, Uppsala University/Uppsala University Hospital, Dag Hammarskjölds väg 20, 751 85, Uppsala, Sweden.

Immunohistochemistry (IHC) is influenced by several factors such as cold ischemia time, fixative, fixation time, paraffin, storage time, antibody, antigen retrieval technique and detection systems. In the setting of post-mortem tissue, not only post-mortem delay, but also agonal state is of interest. Here, we assessed an additional variable, i. Read More

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http://link.springer.com/10.1007/s00418-018-1742-1
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http://dx.doi.org/10.1007/s00418-018-1742-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328518PMC
January 2019
12 Reads

Vascular impairment of adenosinergic system in hypertension: increased adenosine bioavailability and differential distribution of adenosine receptors and nucleoside transporters.

Histochem Cell Biol 2018 Oct 24. Epub 2018 Oct 24.

Laboratory of Pharmacology, Department of Drug Sciences, Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira nº 228, 4050-047, Porto, Portugal.

Adenosinergic system regulates vascular tonicity through the complex system of adenosine, adenosine receptors (ARs) and nucleoside transporters. This work aimed at evaluating the impact of hypertension on adenosine bioavailability and expression/distribution profile of AR subtypes (A, A, A, A) and equilibrative nucleoside transporters (ENT1, ENT2, ENT3, ENT4). Adenosine was measured in vascular tissue extracts by HPLC (fluorescence detection); immunoreactivities (ARs/ENTs) in mesenteric arteries/veins from normotensive Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHR) were analyzed by histomorphometry. Read More

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http://link.springer.com/10.1007/s00418-018-1743-0
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http://dx.doi.org/10.1007/s00418-018-1743-0DOI Listing
October 2018
3 Reads

Serum affects keratinization and tight junctions in three-dimensional cultures of the mouse keratinocyte cell line COCA through retinoic acid receptor-mediated signaling.

Histochem Cell Biol 2019 Apr 17;151(4):315-326. Epub 2018 Oct 17.

Department of Morphological Biology, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka, 814-0193, Japan.

Vitamin A, which is found in serum, is known to affect keratinocyte proliferation, epidermal differentiation, and keratinization. In mice, stratified epithelia in the oral cavity, esophagus, and forestomach are keratinized; however, these epithelia are not keratinized in humans. Several studies have reported that three-dimensional (3D) cultures of human keratinocytes in serum-containing medium could form keratinized epithelia. Read More

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http://dx.doi.org/10.1007/s00418-018-1741-2DOI Listing
April 2019
3 Reads
3.054 Impact Factor

Morphological and molecular criteria allow the identification of putative germ stem cells in a lophotrochozoan, the Pacific oyster Crassostrea gigas.

Histochem Cell Biol 2018 Oct 15. Epub 2018 Oct 15.

Normandy University, Caen, France.

While our knowledge of bivalve gametogenesis recently progressed, data on early stages of gametogenesis remain to be developed, especially when dealing with germinal stem cells (GSC) and their niche in these organisms. Here, we wish to develop a strategy to identify putative GSC in Pacific oyster Crassostrea gigas based on morphological criteria combined with vasa marker expression. A histological quantitative approach, based on stereology, allowed us to identify two types of early germ cells in the germinal epithelium, one presenting round nuclei and the other irregular ones. Read More

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http://dx.doi.org/10.1007/s00418-018-1740-3DOI Listing
October 2018
1 Read

Versican silencing in BeWo cells and its implication in gestational trophoblastic diseases.

Histochem Cell Biol 2019 Apr 8;151(4):305-313. Epub 2018 Oct 8.

Cell Biology Laboratory, Institute of Health and Biological Sciences, Federal University of Alagoas, Campus A.C. Simões. Av. Lourival Melo Mota, s/n, 57072-970, Maceio, Alagoas, Brazil.

Versican is a proteoglycan known to interact with cells to influence their ability to proliferate, differentiate, migrate, invade and assemble extracellular matrix, with all of these cell functions present during placentation. In the placenta, cytotrophoblast cells have the ability to differentiate into the syncytiotrophoblast, a mechanism that is greatly increased in gestational trophoblastic diseases (GTD). Nevertheless, the molecular signaling underlying the increased syncytiotrophoblast differentiation are still being unveiled and may result in novel therapeutic targets for GTD. Read More

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http://link.springer.com/10.1007/s00418-018-1739-9
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http://dx.doi.org/10.1007/s00418-018-1739-9DOI Listing
April 2019
17 Reads

Anisomycin, a JNK and p38 activator, suppresses cell-cell junction formation in 2D cultures of K38 mouse keratinocyte cells and reduces claudin-7 expression, with an increase of paracellular permeability in 3D cultures.

Histochem Cell Biol 2018 Oct 4. Epub 2018 Oct 4.

Department of Morphological Biology, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka, 814-0193, Japan.

Keratinocytes in the oral mucosal epithelium, which is a non-keratinized stratified epithelium, are exposed to various stimuli from the oral cavity. JNK and p38 are stress-activated mitogen-activated protein kinases (MAPKs) that are phosphorylated by various stimuli and are involved in the assembly and disassembly of tight junctions (TJs) in keratinocytes. Therefore, we investigated the effects of stress-activated MAPKs on TJs in a mouse keratinocyte cell line during cell-cell junction formation in two-dimensional (2D) cultures or stratification to form non-keratinized epithelium in 3D cultures. Read More

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http://dx.doi.org/10.1007/s00418-018-1736-zDOI Listing
October 2018
3 Reads
3.054 Impact Factor

Dynamics of WNT signaling components in the human ovary from development to adulthood.

Histochem Cell Biol 2019 Feb 4;151(2):115-123. Epub 2018 Oct 4.

Laboratory for Aging and Infertility Research, Department of Biology, Northeastern University, Boston, MA, 02115, USA.

WNT signaling has been shown to play a pivotal role in mammalian gonad development and sex differentiation; however, its role in the developing human ovary has not been investigated. We analyzed a quantitative mass spectrometry dataset to determine the expression of WNT signaling components between 47 and 137 days of development and in adult ovarian cortex tissue. WNT signaling was identified within the top ten canonical pathways of proteins detected at every developmental stage examined. Read More

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http://link.springer.com/10.1007/s00418-018-1729-y
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http://dx.doi.org/10.1007/s00418-018-1729-yDOI Listing
February 2019
2 Reads

How cancer cells attach to urinary bladder epithelium in vivo: study of the early stages of tumorigenesis in an orthotopic mouse bladder tumor model.

Histochem Cell Biol 2019 Mar 3;151(3):263-273. Epub 2018 Oct 3.

Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.

The majority of bladder cancers in humans are non-muscle-invasive cancers that recur frequently after standard treatment procedures. Mouse models are widely used to develop anti-tumor treatments. The purpose of our work was to establish an orthotopic mouse bladder tumor model and to explore early stages of implantation of cancerous MB49 cells in vivo using various labeling and microscopic techniques. Read More

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http://dx.doi.org/10.1007/s00418-018-1738-xDOI Listing
March 2019
5 Reads
3.054 Impact Factor

Air-blood barrier thickening and alterations of alveolar epithelial type 2 cells in mouse lungs with disrupted hepcidin/ferroportin regulatory system.

Histochem Cell Biol 2019 Mar 3;151(3):217-228. Epub 2018 Oct 3.

Department of Pediatric Hematology, Oncology and Immunology, University of Heidelberg, Heidelberg, Germany.

Iron accumulates in the lungs of patients with common respiratory diseases or transfusion-dependent beta-thalassemia. Based on our previous work, we hypothesized that systemic iron overload affects the alveolar region of the lung and in particular the surfactant producing alveolar epithelial type II (AE2) cells. Mice with a point mutation in the iron exporter ferroportin, a model for human hemochromatosis type 4 were compared to wildtype mice (n = 5 each). Read More

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http://dx.doi.org/10.1007/s00418-018-1737-yDOI Listing
March 2019
5 Reads

In focus in HCB.

Histochem Cell Biol 2018 Nov 29;150(5):403-405. Epub 2018 Sep 29.

University of Zurich, 8091, Zurich, Switzerland.

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http://dx.doi.org/10.1007/s00418-018-1735-0DOI Listing
November 2018
1 Read

Gephyrin: a key regulatory protein of inhibitory synapses and beyond.

Histochem Cell Biol 2018 Nov 27;150(5):489-508. Epub 2018 Sep 27.

Department of Medical Cell Biology, Institute for Anatomy and Cell Biology, Heidelberg University, Heidelberg, Germany.

Scaffolding proteins underlying postsynaptic membrane specializations are important structural and functional components of both excitatory and inhibitory synapses. At inhibitory synapses, gephyrin was identified as anchoring protein. Gephyrin self-assembles into a complex flat submembranous lattice that slows the lateral mobility of glycine and GABA receptors, thus allowing for their clustering at postsynaptic sites. Read More

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http://dx.doi.org/10.1007/s00418-018-1725-2DOI Listing
November 2018
18 Reads