475,216 results match your criteria Histochemistry and cell biology[Journal]


Age- and sex-related changes in cortical and striatal nitric oxide synthase in the Q175 mouse model of Huntington's disease.

Nitric Oxide 2018 Dec 5. Epub 2018 Dec 5.

Department of Neuroscience, The Chicago Medical School at Rosalind Franklin University of Medicine and Science, North Chicago, IL, USA.

In Huntington's disease (HD), corticostriatal and striatopallidal projection neurons preferentially degenerate as a result of mutant huntingtin expression. Pathological deficits in nitric oxide (NO) signaling have also been reported in corticostriatal circuits in HD, however, the impact of age and sex on nitrergic transmission is not well characterized. Thus, we utilized NADPH-diaphorase (NADPH-d) histochemistry and qPCR assays to assess neuronal NO synthase (nNOS) activity/expression in aged male and female Q175 heterozygous mice. Read More

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December 2018

CD5 and CD43 Expression are Associate with Poor Prognosis in DLBCL Patients.

Open Med (Wars) 2018 27;13:605-609. Epub 2018 Nov 27.

Department of Internal Medicine 5th Division Jiangxi Cancer Center, Jiangxi Cancer Hospital, Nanchang University, Nanchang, 330029 P.R. China.

Objective: To investigate the expression and clinical significance of CD5 and CD43 in diffuse large B cell lymphoma (DLBCL) (unspecified).

Methods: Sixty - five patients with diagnosed DLBCL were enrolled. The expressions of CD5, CD43, CD10, Bcl-6 and Mun-1 were detected by immuno histochemistry. Read More

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November 2018
1 Read

Increased Hyaluronan and TSG-6 in Association with Neuropathologic Changes of Alzheimer's Disease.

J Alzheimers Dis 2018 Nov 29. Epub 2018 Nov 29.

Department of Pathology, Division of Neuropathology, University of Washington, Seattle, WA, USA.

Little is known about the extracellular matrix (ECM) during progression of AD pathology. Brain ECM is abundant in hyaluronan (HA), a non-sulfated glycosaminoglycan synthesized by HA synthases (HAS) 1-3 in a high molecular weight (MW) form that is degraded into lower MW fragments. We hypothesized that pathologic severity of AD is associated with increases in HA and HA-associated ECM molecules. Read More

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November 2018
1 Read

Endogenous Nitric Oxide Inhibits, Whereas Awakening Stimuli Increase, the Activity of a Subset of Orexin Neurons.

Biol Pharm Bull 2018 ;41(12):1859-1865

Department of Chemico-Pharmacological Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University.

The lateral hypothalamic area contains neurons expressing neuronal nitric oxide synthase (nNOS), in addition to orexin neurons. Here we examined whether the activity of orexin neurons was regulated by endogenous nitric oxide (NO) in male C57BL/6 mice. Caffeine (30 mg/kg, intraperitoneally (i. Read More

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January 2018

Keratinization and mucogenesis in the epidermis of an angler catfish Chaca chaca (Siluriformes, Chacidae): A Histochemical and fluorescence microscope investigation.

Zoology (Jena) 2018 Dec 11;131:10-19. Epub 2018 Oct 11.

Skin Physiology Laboratory, Centre of Advanced Study, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi 221 005, Uttar Pradesh, India. Electronic address:

The present study describes keratinization and mucogenesis in the epidermis of an angler catfish Chaca chaca, using a series of immunochemical, fluorescence and histochemical methods. The epidermis is primarily mucogenic and shows characteristic specialised structures at irregular intervals. These structures are identified keratinized in nature. Read More

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December 2018

Diagnostic histochemistry in non-neoplastic skin diseases.

Semin Diagn Pathol 2018 Nov 16;35(6):390-398. Epub 2018 Oct 16.

PRW Laboratories, Charlottesville, VA, USA.

Non-neoplastic skin lesions comprise a sizable group of disorders with variable etiologies and clinical manifestations. They can be grouped into vesiculopustular dermatitides; spongiotic and psoriasiform diseases; lichenoid dermatitides; lymphoid infiltrates of the dermis; granulomatous processes; bullous disorders; vasculopathies; panniculitides; deposition disorders; and defects in maintenance of dermal connective tissue. The use of histochemical methods continues to be an indispensable adjunct to conventional microscopy in the further characterization of such lesions. Read More

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November 2018
2 Reads

Chondroitin sulfate isolated from the secretion of the venom-producing parotoid gland of Brazilian bufonid.

Int J Biol Macromol 2018 Nov 27;124:548-556. Epub 2018 Nov 27.

Laboratório de Histologia Integrativa, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil. Electronic address:

The parotoid gland of bufonids is characterized as a specialized integument region, formed by different gland types. The secretion elaborated by the largest glandular alveoli has been related to animal chemical defense and is constituted by granular protein content, associated with a basophilic and alcianophilic material with features of glycoconjugates. This study aimed to identify and characterize the glycoconjugates in the secretion of the largest granular gland of the parotoid gland of Rinella icterica by histochemical and immunohistochemical techniques at light microscopy, biochemical methods, and nuclear magnetic resonance spectroscopy. Read More

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November 2018

The distribution of T-cell subsets and the expression of immune checkpoint receptors and ligands in patients with newly diagnosed and relapsed acute myeloid leukemia.

Cancer 2018 Nov 30. Epub 2018 Nov 30.

Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, Texas.

Background: Phenotypic characterization of immune cells in the bone marrow (BM) of patients with acute myeloid leukemia (AML) is lacking.

Methods: T-cell infiltration was quantified on BM biopsies from 13 patients with AML, and flow cytometry was performed on BM aspirates (BMAs) from 107 patients with AML who received treatment at The University of Texas MD Anderson Cancer Center. The authors evaluated the expression of inhibitory receptors (programmed cell death protein 1 [PD1], cytotoxic T-lymphocyte antigen 4 [CTLA4], lymphocyte-activation gene 3 [LAG3], T-cell immunoglobulin and mucin-domain containing-3 [TIM3]) and stimulatory receptors (glucocorticoid-induced tumor necrosis factor receptor-related protein [GITR], OX40, 41BB [a type 2 transmembrane glycoprotein receptor], inducible T-cell costimulatory [ICOS]) on T-cell subsets and the expression of their ligands (41BBL, B7-1, B7-2, ICOSL, PD-L1, PD-L2, and OX40L) on AML blasts. Read More

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November 2018

CXCL12 regulates differentiation of human immature melanocyte precursors as well as their migration.

Arch Dermatol Res 2018 Nov 27. Epub 2018 Nov 27.

Department of Applied Cell and Regenerative Medicine, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukakecho, Toyoake, Aichi, Japan.

Melanocyte stem cells (McSCs) are localized in the bulge region of hair follicles and supply melanocytes, which determine hair color by synthesizing melanin. Ectopic differentiation of McSCs, which are usually undifferentiated in the bulge region, causes depletion of McSCs and results in hair graying. Therefore, to prevent hair graying, it is essential to maintain McSCs in the bulge region, but the mechanism of McSC maintenance remains unclear. Read More

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November 2018
1 Read

Glial Cells and Retinal Nerve Fibers Morphology in the Optic Nerves of Streptozotocin-induced Hyperglycemic Rats.

J Ophthalmic Vis Res 2018 Oct-Dec;13(4):433-438

Department of Morphology, University of Las Palmas de Gran Canaria, Spain.

Purpose: This study analyzes the structures of optic nerve elements, i.e., glial cells and nerve fibers, in an STZ-induced hyperglycemic animal model. Read More

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November 2018
1 Read

Micro-osteoperforations accelerate orthodontic tooth movement by stimulating periodontal ligament cell cycles.

Am J Orthod Dentofacial Orthop 2018 Dec;154(6):788-796

Department of Orthodontics, School of Dentistry, Nihon University, Matsudo, Chiba, Japan.

Introduction: The aim of this study was to investigate the mechanism of how micro-osteoperforations (MOPs) accelerate tooth movement. We focused on inflammation, cell proliferation, and apoptosis of periodontal ligament cells and performed immunostaining of MOPs exposed to tumor necrosis factor-alpha (TNF-α), proliferating cell nuclear antigen (PCNA), and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) during experimental tooth movement.

Methods: Eleven-week-old male Wistar rats were divided into 2 groups: (1) 10 g of orthodontic force applied to the maxillary first molar (TM) and (2) force application plus 3 small perforations of the cortical plate (TM + MOPs). Read More

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December 2018
6 Reads

Diagnostic histochemistry in neuropathology.

Semin Diagn Pathol 2018 Oct 16. Epub 2018 Oct 16.

Division of Neuropathology, Department of Pathology, University of Virginia Medical Center, Charlottesville, VA, United States. Electronic address:

Neuropathologists have a long and storied history of utilizing histochemistry in the characterization and diagnosis of neurological diseases. This review considers stains that are used for the identification of neurons, neuritic processes and axons, myelin sheaths, neuroglial cells, and connective tissue in the nervous system. Rapid histochemical-smear methods for intraoperative diagnosis are also discussed, along with possible roles for lectin-histochemistry in diagnostic neuropathology. Read More

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October 2018

Papillae revisited and the nature of the adhesive secreting collocytes.

Dev Biol 2018 Nov 22. Epub 2018 Nov 22.

Evolutionary Developmental Biology, Institute of Zoology, University Innsbruck, Technikerstr. 25, A-6020 Innsbruck, Austria; Division of Histology and Embryology, Innsbruck Medical University, Muellerstrasse 59, A-6020 Innsbruck, Austria. Electronic address:

Ascidian papillae (palps) constitute a transient sensory adhesive organ that assures larval settlement and the onset of metamorphosis to the filterfeeding adult. Despite the importance of papillae for the ascidian development, their cellular composition is only roughly described. For Ciona intestinalis/robusta, a clear definition of cell numbers and discriminative molecular markers for the different cell types is missing. Read More

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November 2018
1 Read

Slide-free virtual histochemistry (Part II): detection of field cancerization.

Biomed Opt Express 2018 Nov 5;9(11):5253-5268. Epub 2018 Oct 5.

Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Tumor-adjacent "normal" tissue constitutes a peri-tumoral field that affects early cancer detection, risk assessment, surgical decision, and postoperative surveillance. Modern genetic analysis has revealed valuable information from this field, but without the spatial resolution of optical microscopy to understand the vital microenvironments that surround individual cells. Rapidly advanced optical imaging techniques free of labor-intensive sample preparation, despite great promise to perform slide-free imaging of cell and shift the histology-centered cancer diagnostic paradigm, have lacked compatible and complementary histochemical imaging of cell or to interrogate the peri-tumoral field. Read More

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November 2018
2 Reads

Slide-free virtual histochemistry (Part I): development via nonlinear optics.

Biomed Opt Express 2018 Nov 5;9(11):5240-5252. Epub 2018 Oct 5.

Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Histochemistry is a microscopy-based technology widely used to visualize the molecular distribution in biological tissue. Recent developments in label-free optical imaging has demonstrated the potential to replace the conventional histochemical labels/markers (fluorescent antibodies, organic dyes, nucleic acid probes, and other contrast agents) with diverse optical interactions to generate histochemical contrasts, allowing "virtual" histochemistry in three spatial dimensions without preparing a microscope slide (i.e. Read More

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November 2018
2 Reads

Auditory Midbrain Hypoplasia and Dysmorphology after Prenatal Valproic Acid Exposure.

Neuroscience 2018 Nov 17;396:79-93. Epub 2018 Nov 17.

Department of Anatomy, Lake Erie College of Osteopathic Medicine, Erie, PA, United States. Electronic address:

Prenatal exposure to the antiepileptic valproic acid (VPA) is associated with an increased risk of autism spectrum disorder (ASD) in humans and is used as an animal model of ASD. The majority of individuals with ASD exhibit adverse reactions to sensory stimuli and auditory dysfunction. Previous studies of animals exposed to VPA reveal abnormal neuronal responses to sound and mapping of sound frequency in the cerebral cortex and hyperactivation, hypoplasia and abnormal neuronal morphology in the cochlear nuclei (CN) and superior olivary complex (SOC). Read More

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November 2018
2 Reads

Autotaxin and vascular endothelial growth factor receptor-2 and -3 are related to vascular development during the progression of chronic viral hepatitis C.

APMIS 2018 Dec;126(12):913-921

Organized Center of Clinical Medicine, International University of Health and Welfare, Tokyo, Japan.

Vascular endothelial growth factor (VEGF) and autotaxin (ATX) play important roles in embryonic vasculogenesis and cancer progression. This study examines whether these two angiogenic factors cooperate in the mechanism that regulates vascular development during the progression of chronic viral hepatitis C (CVH-C) (Inuyama classification, F1-F4). First, surgical wedge biopsy specimens and needle biopsy specimens were obtained. Read More

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December 2018
4 Reads

Involvement of HDAC6 in ischaemia and reperfusion-induced rat retinal injury.

BMC Ophthalmol 2018 Nov 20;18(1):300. Epub 2018 Nov 20.

Laboratory of Oral Microbiota and Systemic Diseases, Shanghai Research Institute of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 115 Jin Zun Road, Shanghai, 200125, China.

Background: The role of histone deacetylases 6 (HDAC6) has been elucidated in various neurodegenerative diseases. However, the effect of HDAC6 on retinal degenerative processes remains unknown. The aim of this study was to elucidate the potential role of HDAC6 in the retinal ischaemia and reperfusion (I/R) injury model. Read More

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November 2018
2 Reads

A Proteomic Approach for Understanding the Mechanisms of Delayed Corneal Wound Healing in Diabetic Keratopathy Using Diabetic Model Rat.

Int J Mol Sci 2018 Nov 18;19(11). Epub 2018 Nov 18.

Faculty of Pharmacy, Kindai University, Higashi-Osaka 577-8502, Japan.

Diabetes mellitus is a widespread metabolic disorder, and long-term hyperglycemia in diabetics leads to diabetic keratopathy. In the present study, we used a shotgun liquid chromatography/mass spectrometry-based global proteomic approach using the cornea of streptozotocin-induced diabetic (STZ) rats to examine the mechanisms of delayed corneal wound healing in diabetic keratopathy. Applying a label-free quantitation method based on spectral counting, we identified 188 proteins that showed expression changes of >2. Read More

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November 2018
1 Read

"Man in Istanbul" Lesions of the Urinary Tract (Known Entities in an Unusual Context): Melanoma, Carcinoid Tumors, Epithelioid Angiosarcoma.

Surg Pathol Clin 2018 Dec;11(4):825-836

Department of Pathology, The Johns Hopkins Medical Institutions, Johns Hopkins Hospital, Weinberg 2242, 401 North Broadway, Baltimore, MD 21231-2410, USA; Department of Urology, The Johns Hopkins Medical Institutions, Johns Hopkins Hospital, Weinberg 2242, 401 North Broadway, Baltimore, MD 21231-2410, USA; Department of Oncology, The Johns Hopkins Medical Institutions, Johns Hopkins Hospital, Weinberg 2242, 401 North Broadway, Baltimore, MD 21231-2410, USA. Electronic address:

Certain tumors are more difficult to recognize when they present in an unusual location. Within the urinary tract, primary melanomas, carcinoid tumors, or epithelioid angiosarcoma could present diagnostic challenges due to their infrequent occurrence. This article emphasizes the clinical and histopathologic features of these entities and their differential diagnoses including the immunophenotype and their prognoses. Read More

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December 2018
3 Reads

Contemporary Grading and Staging of Urothelial Neoplasms of the Urinary Bladder: New Concepts and Approaches to Challenging Scenarios.

Surg Pathol Clin 2018 Dec;11(4):775-795

Department of Pathology, University of Chicago, 5841 South Maryland Avenue, AMB S626-MC6101, Chicago, IL 60637, USA; Department of Surgery, Section of Urology, University of Chicago, 5841 South Maryland Avenue, AMB S626-MC6101, Chicago, IL 60637, USA. Electronic address:

Grading and staging of urothelial neoplasm are the most crucial factors in risk stratification and management; both necessitate optimal accuracy and consistency. Several updates and recommendations have been provided though recent publications of the 4th edition of the World Health Organization classification, the 8th edition of the American Joint Committee on Cancer staging system, and the International Consultation on Urological Diseases-European Association of Urology updates on bladder cancer. Updates and recent studies have provided better insights into and approaches to the challenging scenarios in grading and staging of urothelial neoplasm; however, there remain aspects that need further investigation and refinement. Read More

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December 2018
1 Read

A Contemporary Review of Common Adult Non-germ Cell Tumors of the Testis and Paratestis.

Surg Pathol Clin 2018 Dec 17;11(4):739-758. Epub 2018 Oct 17.

Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, L235, Stanford, CA 94305, USA. Electronic address:

This article provides a comprehensive review of non-germ cell tumors of the testis and paratestis in adults, incorporating the latest 2016 World Health Organization updates. Clinical features, gross pathologic findings, key morphologic details, immunohistochemical profiles, and differential diagnoses are covered, with an emphasis on how to resolve commonly encountered, and sometimes difficult, differential diagnoses. Read More

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December 2018
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Recognizing Hereditary Renal Cancers Through the Microscope: A Pathology Update.

Surg Pathol Clin 2018 Dec 17;11(4):725-737. Epub 2018 Oct 17.

Department of Pathology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA. Electronic address:

A heightened understanding of hereditary renal cancer syndromes and their molecular basis has led to an increased awareness and recognition of these renal neoplasms by pathologists. Because a diagnosis of hereditary renal cell carcinoma has a profound impact on the patient and family members, when and how to raise such a suspicion via pathologic assessment has become an important yet very challenging task. This review discusses key clinicopathologic, immunohistochemical, and genetic characteristics of hereditary renal cancer syndromes, and important differential diagnostic challenges, emphasizing recent pathologic and molecular advances. Read More

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December 2018
4 Reads

How the activity of natural enemies changes the structure and metabolism of the nutritive tissue in galls? Evidence from the Palaeomystella oligophaga (Lepidoptera) -Macairea radula (Metastomataceae) system.

Protoplasma 2018 Nov 16. Epub 2018 Nov 16.

Laboratório de Anatomia, Desenvolvimento Vegetal e Interações (LADEVI); Instituto de Biologia, Universidade Federal de Uberlândia-UFU, Uberlândia, Brazil.

Insect-induced galls usually develop nutritional cells, which they induce and consume directly, and any metabolic modification of those cells may reflect changes of the insect's own metabolism. The system Palaeomystella oligophaga (Lepidoptera)-Macairea radula (Melastomataceae) presents a series of natural enemies, including parasitoids and cecidophages that can function as a natural experiment, respectively removing the specific galling feeding stimulus and providing a nonspecific one. Considering that the process of induction and maintenance of gall tissues strictly depends on the constant specific stimulus of galling, question I:What kind of metabolic changes these different groups of natural enemies can promote in chemical and structural composition of these galls? II: How the specialized tissues are metabolically dependent on the constant specific stimulus of galling in latter stages of gall development? Galls without natural enemies, with parasitoids or cecidophages in larvae or pupae stages were analyzed through histochemistry and cytological profiles and all compared to galls in natural senescence state. Read More

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November 2018
2 Reads

Reducing the Radiotherapy Dose Prescription for Elective Treatment Areas in Human Papillomavirus (HPV)-Associated Oropharyngeal Carcinoma (OPC) Being Treated with Primary Chemoradiotherapy at XXXXX.

Pract Radiat Oncol 2018 Nov 13. Epub 2018 Nov 13.

Department of Radiation Oncology, Memorial Sloan Kettering Cancer Center, New York, New York. Electronic address:

Data on the biology and radiosensitivity of HPV-related cancers has led many expert radiation oncologists to decrease the radiation dose to target areas in selected situations. At XXXXX, we have altered our radiation guidelines to allow substantial dose reduction to elective treatment; regions and to omit radiation completely in other regions that have historically been included in; the elective treatment volume. Here we summarize our current dose prescriptions and briefly explain the rationale behind these choices, which we anticipate will change when additional data matures. Read More

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November 2018

Intraoperative diagnosis of the head and neck lesions, thyroid and parathyroid gland, bone and soft tissue, and genitourinary tract.

Cesk Patol 2018 ;54(2):72-80

In this article, indications and pitfalls in frozen section diagnosis in selected organs and systems are discussed. The main indications for frozen section examination of head and neck and genitourinary system lesions are to evaluate the resection margin and the metastatic involvement of lymph nodes. Recently, intraoperative consultation has been introduced for identification of patients who might benefit from testis-sparing surgery. Read More

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January 2018
3 Reads

Effects of cyclosporin, nifedipine and phenytoin on gingival myofibroblast transdifferentiation in monkeys.

J Appl Oral Sci 2018 Nov 8;27:e20180135. Epub 2018 Nov 8.

Univ. Estadual Paulista, Faculdade de Odontologia, Departamento de Patologia e Propedêutica Clínica, Araçatuba, São Paulo, Brasil.

Objective: Myofibroblasts have been associated with the development of several pathologic fibrotic conditions. This longitudinal study aims to assess the proliferative and antiapoptotic effects of cyclosporin, nifedipine and phenytoin on gingival connective tissue cells of nonhuman primate, as well as to analyze a possible role of myofibroblasts in gingival overgrowth.

Materials And Methods: Gingival samples from the right superior canine area were obtained from 12 male monkeys ( Sapajus spp ) to comprise the control group. Read More

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November 2018
2 Reads

Phosphoinositide-specific phospholipase C in normal human liver and in alcohol abuse.

J Cell Biochem 2018 Nov 13. Epub 2018 Nov 13.

Department of Sensory Organs, Sapienza University of Rome, Rome, Italy.

The phosphoinositide (PI) signal transduction pathway participates in liver metabolism. Abnormal activity or expression of PI-specific phospholipase C (PLC) enzymes has been described in different liver diseases. We resume the role of the PI metabolism in liver and PLC abnormalities in different liver diseases. Read More

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November 2018
4 Reads

Ultrastructural histochemistry in biomedical research: Alive and kicking.

Eur J Histochem 2018 Nov 7;62(4). Epub 2018 Nov 7.

University of Verona, Department of Neurosciences, Biomedicine and Movement Sciences.

The high-resolution images provided by the electron microscopy has constituted a limitless source of information in any research field of life and materials science since the early Thirties of the last century. Browsing the scientific literature, electron microscopy was especially popular from the 1970's to 80's, whereas during the 90's, with the advent of innovative molecular techniques, electron microscopy seemed to be downgraded to a subordinate role, as a merely descriptive technique. Ultrastructural histochemistry was crucial to promote the Renaissance of electron microscopy, when it became evident that a precise localization of molecules in the biological environment was necessary to fully understand their functional role. Read More

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November 2018

LACE-Bio: Validation of Predictive and/or Prognostic Immunohistochemistry/Histochemistry-based Biomarkers in Resected Non-small-cell Lung Cancer.

Clin Lung Cancer 2018 Oct 11. Epub 2018 Oct 11.

Departments of Medical Oncology and Hematology (FAS) and Pathology (M-ST), University Health Network, Princess Margaret Cancer Centre, and the University of Toronto, Toronto, Ontario, Canada.

Background: Complete resection of non-small-cell lung cancer (NSCLC) offers the potential for cure after surgery and adjuvant chemotherapy. Patients may not benefit and may experience severe toxicity. There are no validated molecular tools to allow better patient selection. Read More

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October 2018
3 Reads

Total Bioavailable Organic Selenium in Fishmeal-Based Diet Influences Growth and Physiology of Juvenile Cobia Rachycentron canadum (Linnaeus, 1766).

Biol Trace Elem Res 2018 Nov 9. Epub 2018 Nov 9.

School of Molecular and Life Sciences, Curtin University, Bentley, WA, 6102, Australia.

The study examined the effects of supplemental organic selenium (Se) extracted from selenoyeast on the growth performance, glutathione peroxidase (GPx) activity, biochemical status and liver histochemistry of juvenile cobia Rachycentron canadum. Six experimental diets were prepared supplemented with Se with total concentration of 1.52 (Se-1. Read More

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November 2018
3 Reads

Prediction model of lymphovascular invasion based on clinicopathological factors in Chinese patients with invasive breast cancer.

Medicine (Baltimore) 2018 Oct;97(43):e12973

Thoracic Surgery, The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, Qingyuan.

The aim of this study was to evaluate the correlation between lymphovascular invasion (LVI) and tumor size, histological grade, and the expression statuses of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor-2 (HER-2), Ki67, epidermal growth factor receptor (EGFR), vascular endothelial growth factor (VEGF), E-cadherin, and P53 in invasive breast cancer, then establish a prediction model of LVI based on the associated clinicopathological factors.A total of 392 patients with primary invasive breast cancers were enrolled, and their paraffin-embedded tissues were manufactured into the tissue microarray. We evaluated the expression statuses of ER, PR, HER-2, Ki67, EGFR, VEGF, E-cadherin, and P53 based on immunohistochemistry, histological grade and LVI based on the hematoxylin and eosin stain, and tumor size. Read More

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October 2018
3 Reads

Clinicopathologic features of endometrial cancer in Chinese patients younger than 50 years with a family history of cancer.

Medicine (Baltimore) 2018 Oct;97(43):e12968

Obstetrics and Gynecology Hospital of Fudan University.

Genetic factors play an important role in shaping the biologic characteristics of malignant tumors, especially in young patients. We aimed to determine the clinicopathologic features of endometrial cancer (EC) in patients younger than 50 years with a family history of cancer.Overall, 229 patients with EC, including 40 with a positive family history of cancer (PFH) and 189 with a negative family history of cancer (NFH), were enrolled in this case-control study. Read More

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October 2018
4 Reads

Correlation of tumor uptake on breast-specific gamma imaging and fluorodeoxyglucose PET/CT with molecular subtypes of breast cancer.

Medicine (Baltimore) 2018 Oct;97(43):e12840

Department of Nuclear Medicine.

Mechanisms of technetium-99m sesta-methoxyisobutylisonitrile (sestamibi) and F-fluorodeoxyglucose (FDG) uptake by tumor are different. The purpose of this study was to investigate the association between the tumor uptake of these 2 tracers in invasive ductal carcinoma and to examine thecorrelation of uptake of each tracer with prognostic factors and tumor molecular subtypes.A total of 96 patients with invasive ductal carcinoma who underwent preoperative breast-specific gamma imaging and FDG positron-emission tomography/computed tomography were retrospectively enrolled. Read More

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October 2018
3 Reads

Diagnostic histochemistry in hepatic pathology.

Authors:
Julia C Iezzoni

Semin Diagn Pathol 2018 Nov 16;35(6):381-389. Epub 2018 Oct 16.

Division of Surgical Pathology and Cytopathology, Department of Pathology, University of Virginia Health System, P.O. Box 800214, Charlottesville, VA 22908, United States. Electronic address:

Histochemistry has an important, continuing role in the current assessment of hepatic biopsies and resection specimens. The evaluation of connective tissue elements in the liver can be accomplished with such methods as the Masson trichrome, Snook reticulin, Vierhoff van Gieson, orcein, and Victoria blue stains. The results contribute to the diagnosis of acute and chronic hepatitis, submassive necrosis, venous outflow obstruction, steatohepatitis, and cirrhosis. Read More

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November 2018
6 Reads

Glomus tumor of the stomach: A case report.

Medicine (Baltimore) 2018 Nov;97(45):e13132

Department of Gastric and Colorectal Surgery, The First Hospital of Jilin University.

Rationale: Glomus tumors (GTs) are a rare disorder originating from the glomus of the anastomoses of small arteries, usually occurring in the subungual region of the fingertips or toes and seldom occurring in the stomach. We unintentionally found a case of a gastric glomus tumor (GGT) without any upper abdominal discomfort. The diagnosis of this disease was mainly by immunohistochemistry. Read More

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November 2018
4 Reads

The use of decellularised animal tissue to study disseminating cancer cells.

J Cell Sci 2018 Dec 10;132(4). Epub 2018 Dec 10.

Department of Biochemistry, School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK

Since the establishment of cell culture, common practice has been to grow adherent cells in 2D monolayers. Although cells behave completely differently when grown under these artificial conditions, the ease of 2D culturing has meant that this practice still prevails, and adopting conditions that more closely reflect the natural microenvironment has been met with substantial inertia. The alternative, animal models that mimic natural human physiology, are less accessible, strictly regulated and require licences and expensive facilities. Read More

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December 2018
3 Reads

Quantitative proteomic analysis of aqueous humor from patients with drusen and reticular pseudodrusen in age-related macular degeneration.

BMC Ophthalmol 2018 Nov 7;18(1):289. Epub 2018 Nov 7.

Department of Ophthalmology, Konkuk University School of Medicine, Konkuk University Medical Center, 120-1 Neungdong-ro, Gwangjin-gu, Seoul, Republic of Korea.

Background: To identify novel biomarkers related to the pathogenesis of dry age-related macular degeneration (AMD), we adopted a human retinal pigment epithelial (RPE) cell culture model that mimics some features of dry AMD including the accumulation of intra- and sub-RPE deposits. Then, we investigated the aqueous humor (AH) proteome using a data-independent acquisition method (sequential window acquisition of all theoretical fragment ion mass spectrometry) for dry AMD patients and controls.

Methods: After uniformly pigmented polarized monolayers of human fetal primary RPE (hfRPE) cells were established, the cells were exposed to 4-hydroxy-2-nonenal (4-HNE), followed by Western blotting, immunofluorescence analysis and ELISA of cells or conditioned media for several proteins of interest. Read More

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November 2018
2 Reads

Amyloid from a histochemical perspective. A review of the structure, properties and types of amyloid, and a proposed staining mechanism for Congo red staining.

Authors:
Richard W Dapson

Biotech Histochem 2018 7;93(8):543-556. Epub 2018 Nov 7.

a Dapson and Dapson LLC , Richland , Michigan , USA.

Amyloid is a diverse group of unrelated peptides or proteins that have positive functionality or are associated with various pathologies. Despite vast differences, all amyloids share several features that together uniquely define the group. 1) All amyloids possess a characteristic cross-ß pattern with X-ray diffraction typical of ß-sheet secondary protein structures. Read More

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November 2018
2 Reads

Significance of M2 macrophage in tubulointerstitial disease secondary to primary Sjogren's disease.

Ren Fail 2018 Nov;40(1):634-639

c Department of Nephrology , Clinical Medical College, Yangzhou University , Yangzhou , China.

Objective: M2 Macrophages could improve tubulointerstitial disease in animal models. HIF-1αpromotes macrophage polarization and is involved in tubular injury. The study aims to observe the clinicopathologic significance of M2 macrophage and HIF-1α in tubulointerstitial injury secondary to primary Sjogren's disease. Read More

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November 2018
7 Reads
0.775 Impact Factor

Very rare localization of a retroperitoneal hemangiopericytoma revealed by lumbosciatalgia: A case report.

Int J Surg Case Rep 2018 Oct 30;53:127-131. Epub 2018 Oct 30.

Department of Surgery, Sahloul Hospital, Sousse, Tunisia. Electronic address:

Introduction: Hemangiopericytoma is a rare vascular tumor representing about 1% of all vascular tumors and approximatively 5% of all soft tissues sarcomas that arises from the pericytes of Zimmerman surrounding capillaries and postcapillaries vessels. Retroperitoneal hemangiopericytomas are rare among the localisations as it usually occurs in lower and upper extremities.

Presentation Of Case: We present a case of 31-year-old man with a well-defined retro peritoneal mass measuring about 105 × 73 × 83 mm at right lower quadrant of the abdomen anterior to right psoas muscle. Read More

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October 2018
3 Reads

The renaissance of human skin organ culture: A critical reappraisal.

Differentiation 2018 Nov - Dec;104:22-35. Epub 2018 Oct 16.

Department of Dermatology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. Electronic address:

Human skin organ culture (hSOC) is a simple but highly instructive and clinically relevant skin research method. It has been used for decades to study the development, differentiation, and function as well as the response to wounding or test agents of intact human skin in the presence of its appendages and all resident cell populations. hSOC has also proven useful in toxicological and oncological studies and studies of skin aging (both chronological aging and photoaging), skin energy metabolism, skin immunology, pigmentation biology, and cutaneous (neuro-)endocrinology and neurobiology. Read More

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October 2018
4 Reads

Activography reveals aberrant proteolysis in desquamating diseases of differing backgrounds.

Exp Dermatol 2018 Nov 3. Epub 2018 Nov 3.

Department of Pharmacy, School of Health Sciences, University of Patras, Rion-Patras, 265 04, Greece.

The role of epidermal proteolysis in overdesquamation was revealed in Netherton syndrome, a rare ichthyosis due to genetic deficiency of the LEKTI inhibitor of serine proteases. Recently, we developed activography, a new histochemical method, to spatially localize and semi-quantitatively assess proteolytic activities using activity-based probes. Activography provides specificity and versatility compared to in situ zymography, the only available method to determine enzymatic activities in tissue biopsies. Read More

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November 2018

Expression of estrogen receptor, progesterone receptor, human epidermal growth factor receptor 2, and Ki-67 in ductal carcinoma in situ (DCIS) and DCIS with microinvasion.

Medicine (Baltimore) 2018 Nov;97(44):e13055

Department of Pathology, Guangdong Women and Children Hospital.

Ductal carcinoma in situ (DCIS) represents a heterogeneous disease in its histologic appearance and biological potential. Some women treated for DCIS subsequently develop invasive breast cancer. DCIS with microinvasion is considered as the interim stage in the progression from DCIS to invasive breast cancer. Read More

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November 2018
1 Read

Correlation between tripartite motif-containing protein 44 protein expression and the prognosis of postoperative patients exhibiting skin squamous cell carcinoma.

Medicine (Baltimore) 2018 Nov;97(44):e13021

College of pharmacy, Jiangsu Vocational College Medicine, Yancheng, Jiangsu, China.

Tripartite motif-containing protein 44 (TRIM44) plays an important role in the development and progression of some human cancers; however, its role in skin squamous cell carcinoma (SCC) remains unknown. The aim of the present study was to investigate TRIM44 expression and clinicopathological significance of TRIM44 in SCC.Immunohistochemistry (IHC) technique, reverse transcriptase-polymerase chain reaction (RT-PCR) and western blot were performed to evaluate differences in TRIM44 protein expression in SCC and normal skin tissues. Read More

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November 2018
5.720 Impact Factor

Purinergic Signaling Modulates Survival/Proliferation of Human Dental Pulp Stem Cells.

J Dent Res 2018 Nov 1:22034518807920. Epub 2018 Nov 1.

1 Department of Dentistry, Eastman Institute for Oral Health, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA.

Human dental pulp stem cells (hDPSCs) reside in postnatal dental pulp and exhibit the potential to differentiate into odontoblasts as well as neurons. However, the intercellular signaling niches necessary for hDPSC survival and self-renewal remain largely unknown. The objective of this study is to demonstrate the existence of intercellular purinergic signaling in hDPSCs and to assess the impact of purinergic signaling on hDPSC survival and proliferation. Read More

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November 2018
8 Reads

Different Glycoconjugate Content in Mucus Secreting Cells of the Rat Fundic Gastric Glands.

Anat Rec (Hoboken) 2018 Dec 1;301(12):2128-2144. Epub 2018 Nov 1.

Department of Cell Biology and Histology, Faculty of Medicine and Nursing, University of the Basque Country UPV/EHU, Leioa, Vizcaya, Spain.

The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands. They produce mucins, highly glycosylated proteins. Very little is known about the glycan composition of these mucins and of gastric secretion in general. Read More

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December 2018
3 Reads

Phenotypic Modulation of Skeletal Muscle Fibers in LPIN1-Deficient Lipodystrophic ( fld) Mice.

Vet Pathol 2018 Oct 31:300985818809126. Epub 2018 Oct 31.

3 Department of Medicine (Endocrinology), Albert Einstein College of Medicine, Bronx, NY, USA.

Lipin-1 ( Lpin1)-deficient lipodystrophic mice have scant and immature adipocytes and develop transient fatty liver early in life. Unlike normal mice, these mice cannot rely on stored triglycerides to generate adenosine triphosphate (ATP) from the β-oxidation of fatty acids during periods of fasting. To compensate, these mice store much higher amounts of glycogen in skeletal muscle and liver than wild-type mice in order to support energy needs during periods of fasting. Read More

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October 2018
15 Reads

Immunohistochemical expression of RANKL in oral giant cell lesions is predictive of aggressiveness.

Braz Oral Res 2018 Oct 29;32:e115. Epub 2018 Oct 29.

Universidade Federal de Santa Catarina - UFSC, Department of Pathology, Florianópolis, Santa Catarina, Brazil.

The aim of this study was to evaluate the immunohistochemical expression of receptor activator of nuclear factor kappa-B ligand (RANKL) and of osteoprotegerin (OPG), important proteins correlated with osteoclastogenesis, in central giant cell lesions (CGCL) and peripheral giant cell lesions (PGCL) and to compare their expression with the histological and clinical parameters for quantification of multinucleated giant cells (MGC) and their nuclei, lesion size, and recurrences. Twenty cases of each lesion type were selected to quantify the number of MGCs and nuclei/mm2 of connective tissue. The immunoreactivity of RANKL and OPG was expressed as a percentage of the marked area in the stroma. Read More

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October 2018

The effects of smoking on the expression of gelatinases in chronic periodontitis: a cross-sectional study.

Braz Oral Res 2018 Oct 25;32:e114. Epub 2018 Oct 25.

Baskent University, Faculty of Medicine, Department of Pathology, Bahçelievler, Ankara, Turkey.

Smokers have a risk of developing periodontal disease. Matrix metalloproteinases (MMP) play a significant role in periodontal tissue destruction. In this study possible relationship between smoking and gingival tissue expression of gelatinases in chronic periodontitis patients relative to periodontally healthy subjects was investigated. Read More

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October 2018
1 Read