2,644 results match your criteria Histochemical Journal[Journal]


Glycosylation of the male genital ducts and spermatozeugmata formation in the clearnose skate Raja eglanteria.

Histochem J 2002 ;34(11-12):601-15

Academic Unit of Obstetrics and Gynaecology, School of Medicine, University of Manchester St Mary's Hospital, Whitworth Park, Manchester M13 0JH, UK.

Genital ducts of three male Raja eglanteria were fixed and embedded in epoxy and methacrylate resin. Epoxy resin sections from the Leydig gland, upper and lower epididymis, ductus deferens and seminal vesicle were stained with 20 labelled lectins to examine their glycosylation. The Leydig gland consisted of columnar epithelial cells expressing N-linked glycans, N-acetyl galactosamine, glucosamine and lactosamine residues and sialic acid. Read More

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June 2004
2 Reads

Expression pattern of glycoconjugates in rat retina as analysed by lectin histochemistry.

Histochem J 2002 ;34(11-12):589-600

Department of Anatomy, the Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, People's Republic of China.

The present study sought to characterize the expression and distribution of complex glycoconjugates in the rat retina by lectin histochemistry, using a panel of 21 different lectins with different carbohydrate specificities. Paraffin sections of Carnoy-fixed Sprague-Dawley rat eyes were stained with various biotinylated lectins, followed by the streptavidin-peroxidase and glucose oxidase-diaminobenzidine-nickel staining procedures. The results showed that the retinal pigment epithelium was stained intensely with LCA, Jacalin, WFA, S-WGA, PWA, DSA, UEA-I, LTA and PHA-E, suggesting that this epithelium contained glycoconjugates with alpha-Man, alpha-Glc, alpha-Gal/GalNAc, beta-GalNAc, alpha-Fuc, NeuAc and other oligosaccharide residues. Read More

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June 2004
4 Reads

Autometallographic localization of heavy metals in resin sections, cryosections and isolated cells of the mussel Mytilus galloprovincialis (L.).

Histochem J 2002 ;34(11-12):583-8

Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece.

In the present study, autometallography (AMG) has been performed on resin-embedded tissue sections, cryosections, as well as on isolated cells from the digestive gland, the gills and the haemolymph of mussels (Mytilus galloprovincialis) collected from the field. The AMG reaction was more intense in cryosections and isolated cells compared to resin sections. This intense AMG reaction found in cryosections and in isolated cells and the time effectiveness of the procedures enhances their use in a variety of studies, such as biomonitoring, even if small amount of metals are to be detected. Read More

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June 2004
4 Reads

Changes in the thymus of peripubertal rats induced by centrally applied somatostatin-28.

Histochem J 2002 ;34(11-12):573-82

Immunology Research Center Branislav Jankovic, Institute of Immunology and Virology Torlak, Belgrade, Yugoslavia.

The aim of this study was to investigate the effects of centrally applied somatostatin-28 on morphometric characteristics of the thymus, the thymocyte subpopulations, as well as, on apoptosis and phases of cell cycle in thymocytes. For this purpose, peripubertal male rats were cannulated intracerebroventriculary and treated with repeated, nanomolar concentrations of somatostatin-28 (experimental group) or saline (control group). Animals were sacrificed and their thymuses were used for the analysis of thymocyte subpopulations, cell cycle and apoptosis by flow cytometry and for the evaluation of morphometric parameters by stereological analysis. Read More

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June 2004
21 Reads

Localization of endogenous biotin-containing proteins in mouse Bergmann glial cells.

Histochem J 2002 ;34(11-12):567-72

Department of Anatomy, Faculty of Medicine, University of Yamanashi, Tamaho, Yamanashi 409-3898, Japan.

A peroxidase-conjugated avidin-biotin complex was used to detect endogenous biotin-containing proteins in mouse cerebellum. By this method, Bergmann glial cells were found to be strongly labelled in the adult mouse cerebellum. Developmentally, cells in the granular layer, probably astrocytes, appeared to be labelled around postnatal 10-day (P10). Read More

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June 2004
2 Reads

Immunolocalization of laminin-alpha1-like antigens around synapses in mouse cerebellar perineuronal nets.

Histochem J 2002 ;34(11-12):559-65

Department of Anatomy, Faculty of Medicine, University of Yamanashi, Tamaho, Yamanashi 409-3898, Japan.

The hypothesis that extracellular matrix components may be related to neuronal development in the mouse cerebellar cortex was verified with immunohistochemistry by using an antibody against laminin-alpha1, a major extracellular matrix protein in various tissues. A commercially available polyclonal antibody, raised against the carboxyl-terminal 20-amino acid peptide of laminin-alpha1 was used. Some positive immunoreaction products were localized around large GABAergic interneurons in granular layers and others were around neurons in deep cerebellar nuclei. Read More

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June 2004
28 Reads

Mast cells are present in epithelial layers of different tissues of the mollusc Anomalocardia brasiliana. In situ characterization of heparin and a correlation of heparin and histamine concentration.

Histochem J 2002 ;34(11-12):553-8

Departamento de Bioquimica, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.

Heparin, other sulphated glycosaminoglycans and histamine were extracted from various dissected organs of Anomalocardia brasiliana, a mollusc from the South Atlantic, and quantified. A good correlation between heparin and histamine content was found in the labial palp, intestine, ctenidium, mantle and foot tissues. The tissue location of metachromatic cells, putatively containing heparin, was identified histologically with Alcian Blue, Toluidine Blue, Masson trichrome, Haematoxylin-Eosin and PAS. Read More

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June 2004
3 Reads

Tyrosine phosphorylation in human lymphomas.

Histochem J 2002 ;34(11-12):545-52

Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, Oxford, UK.

In a previous study, we showed that the high level of protein tyrosine phosphorylation present in lymphomas containing an anaplastic lymphoma kinase (ALK) can be demonstrated in routinely processed paraffin tissue sections using immunolabelling techniques. In the present study we investigated whether oncogenic tyrosine kinase activation also occurs in other categories of lymphoma by staining 145 cases of lymphoma covering those tumours with a range of different subtypes including those with morphological similarity to ALK-positive anaplastic large cell lymphoma (ALCL). Twelve cases of the borderline malignant disorder lymphomatoid papulosis were also studied. Read More

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June 2004
4 Reads

Immunohistochemical localization of hemicelluloses and pectins varies during tissue development in the bamboo culm.

Histochem J 2002 ;34(11-12):535-44

Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan.

The distribution of hemicelluloses and pectins in bamboo internodes was studied immunocytochemistrically at various stages of development. The ultra-structures of bamboo cell walls have been reported previously at various stages. The internodes were identically classified into three developmental phases: primary wall stage (phase I), unlignified secondary wall stage (phase II) and lignified wall stage (phase III), using the same bamboo culm. Read More

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June 2004
4 Reads

Lipolytic and esterolytic activities in posterior lingual glands of rat: a histochemical study.

Histochem J 2002 ;34(11-12):529-33

Department of Oral Pathology, The University of Liverpool School of Dentistry, Pembroke Place, Liverpool L3 5PS, UK.

Catalytic activities of lingual lipase were investigated by enzyme histochemistry in post-mortem tongues from male rats. Sections of fresh-frozen or formalin-calcium fixed tissue were incubated with naphthol-AS-nonanoate and alpha-naphthyl acetate substrate mixtures. The effects of pH level, sodium taurocholate activator and E600 inhibitor were also examined. Read More

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June 2004
3 Reads

Route of jejunal mucosa absorption of trypsin demonstrated by immunofluorescence.

Histochem J 2002 ;34(11-12):525-8

Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.

Previous studies have demonstrated the absorption of porcine trypsin in isolated jejunal loops from male Wistar rats by open-loop perfusion. The possible routes of absorption were examined in the study reported here. Trypsin (0. Read More

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June 2004
3 Reads

Histochemical investigations on the secretory cells in the oesophagogastric tract of the Eurasian green toad, Bufo viridis.

Histochem J 2002 Oct;34(10):517-24

Department of Zoology, Laboratory of Histology and Comparative Anatomy, University of Bari, Bari, Italy.

The secretory cells of the oesophagogastric tract of the Eurasian toad, Bufo viridis, were examined using standard histochemical methods and lectin histochemistry. Two goblet cell types were found in the oesophageal epithelium, differing in their morphology and the histochemical features of the secretory granules. These contained mainly acidic glycoconjugates, both sulphated and carboxylated, and a small amount of pepsinogen. Read More

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October 2002
3 Reads

Langerhans cell-like dendritic cells in the cornea, tongue and oesophagus of the chicken (Gallus gallus).

Histochem J 2002 Oct;34(10):507-15

Departamento de Biología Celular y Tisular, Facultad de Medicina, Instituto de Fisología Celular Universidad Nacional Autónoma de México, México D.F. CP 04510.

Langerhans cells are dendritic leucocytes which reside mainly within stratified squamous epithelia of skin and mucosa. Their visualization requires the use of ATPase histochemistry, electron microscopy for identifying the unique trilaminar cytoplasmic organelles (the Langerhans cell granules or Birbeck granules), and the expression of major histocompatibility complex class II molecules. Following uptake of antigen, Langerhans cells migrate via the afferent lymphatics to the lymph nodes and undergo differentiation from an antigen-processing cell to an antigen-presenting cell. Read More

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October 2002
4 Reads

Hyperplasia of myoepithelial cells expressing calponin during atrophy of the rat parotid gland induced by duct ligation.

Histochem J 2002 Oct;34(10):499-506

Laboratory of Oral Pathology, Dentistry School, Federal University of Rio Grande do Norte, 1787-CEP-59056-000 Natal, Brazil.

The number and location of myoepithelial cells in the rat parotid gland submitted to surgical ligation of its main excretory duct were studied through immunohistochemical labelling for calponin. These cells were labelled by the streptavidin-biotin method using anti-calponin primary antibody, and their number was determined during each step of glandular atrophy, i.e. Read More

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October 2002
3 Reads

Phenotypic variations and dynamic topography of transformed cells in an experimental model of diethylstilbestrol-induced renal tumour in male Syrian hamster.

Histochem J 2002 Oct;34(10):487-97

Service d'Histologie, Faculté de Médecine et de Pharmacie, Université de Mons-Hainaut, Avenue du Champ de Mars, B-7000 Mons, Belgium.

This work explores the phenotypic changes affecting transformed cells in an experimental model of diethylstilbestrol (DES)-induced renal tumours in male Syrian hamster. This estrogen-induced neoplasm presents an important cytological pleomorphism and its origin remains largely controversial. In order to characterize phenotypic variations during tumour progression, the occurrence of seven lineage markers was analysed by a morphometric approach in kidney sections of DES-exposed hamsters (6-11 months). Read More

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October 2002
6 Reads

Quantitative analysis of type IV collagen alpha chains in the basement membrane of human urogenital epithelium.

Histochem J 2002 Oct;34(10):479-86

Department of Surgical Pathology, Kumamoto University School of Medicine, 1-1-1 Honjo, Kumamoto 860-8556, Japan.

Type IV collagen is a major component of the basement membrane (BM), which consists of six genetically distinct alpha(IV) chains. In this study the expression of these six alpha(IV) chains was demonstrated immunohistochemically. In addition, the alpha2(IV) and alpha5(IV) chains were analysed quantitatively by confocal laser scanning microscopy in human urogenital epithelial BM. Read More

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October 2002
11 Reads

Endothelin in the middle cerebral artery: a case of multiple system atrophy.

Histochem J 2002 Oct;34(10):469-77

Department of Anatomy and Developmental Biology, University College London, Gower Street, London, WC1E 6BT, UK.

In this study, we show the changes in the wall of the middle cerebral artery of a subject who suffered multiple system atrophy with autonomic failure. An electron-immunocytochemical approach was employed to reveal the presence of endothelin-1. Our results demonstrate the presence of immunoreactive endothelin-1 in the endothelial cells of the intima, vascular smooth muscle cells and macrophages of the media and neointima, and perivascular nerves/axons varicosities at the adventitial-medial border of the artery. Read More

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October 2002
2 Reads
1 Citation

A novel embedment-free immunoelectron microscopy technique reveals association of apoptosis-regulating proteins with subcellular structures.

Histochem J 2002 Aug-Sep;34(8-9):441-6

Laboratory of Cell Ultrastructure, Medical Research Centre, Polish Academy of Sciences, 5 Pawinski Street, 02 106 Warsaw, Poland.

By employing two electron microscopy techniques, postembedding double- and triple immunocytochemical gold-labelling combined with embedment-free electron microscopy (EF-EM), we have detected previously unreported nuclear and cytoplasmic complexes between different proapoptatic proteins in a human cancer cell line COLO 205 stimulated to apoptosis by nimesulide, a specific cyclooxygenase-2 inhibitor. Experiments with the use of double- and triple immunolabelling visualized the colocalization of proapoptotic proteins such as Bax with Bid, Bax with Bid and voltage-dependent anion channel protein (VDAC-1), and Bax with Bid and caspase-8, on organellar membranes and within the nucleus. Application of this technique in combination with EF-EM technique augments our knowledge on the precise identification and relationship of subcellular structures containing Bax, Bid, VDAC-1 and caspase-8. Read More

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August 2003
4 Reads

Localization of putative neurotransmitters in the mantle and siphuncle of the mollusc Nautilus pompilius L. (Cephalopoda).

Histochem J 2002 Aug-Sep;34(8-9):435-40

Institut für Allgemeine Zoologie und Enwicklungsbiologie, Justus-Liebig-Universität Giessen, Stephanstrasse 24, 35390 Giessen, Germany.

The neurotransmitter supply in the nerve endings of the mantle and the siphuncle, i.e. in organs that are responsible for the shell formation in the ectocholeate Nautilus pompilius, were investigated with electron microscopical, fluorescence-, immuno- and enzyme histochemical methods as well as with high pressure liquid chromatography (HPLC). Read More

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August 2003
2 Reads

Immunocytochemistry of glycine in small neurons of the granule cell areas of the guinea pig dorsal cochlear nucleus: a post-embedding ultrastructural study.

Authors:
Lorenzo Alibardi

Histochem J 2002 Aug-Sep;34(8-9):423-34

Dipartimento di Biologia evoluzionistica sperimentale, University of Bologna, via Selmi 3, 40126, Bologna, Italy.

The axon terminals of the acoustic nerve contact different part of the cochlear nucleus including granule cell areas. Little is known of the cell composition and neural circuits of granule cell areas present in the fusiform and upper polymorphic layers of the dorsal cochlear nucleus in the guinea pig. The present ultrastructural immunocytochemical study exploits the technique of post-embedding immunogold and silver intensification to reveal the characteristics of small neurons in granule cell areas. Read More

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August 2003
4 Reads

Evidence of a bi-phasic effect of thrombospondin-1 on angiogenesis.

Histochem J 2002 Aug-Sep;34(8-9):411-21

Unit of Cell and Molecular Biology, Dental School, University of Dundee, Dundee DD1 4HR, Scotland.

Contradictory results have been reported regarding the association between vascularity (used as an index of angiogenesis) and thrombospondin-1 (TSP-1) in human tumours. In previous studies, the reported association was based on the estimated average TSP-1 value per tumour, with a sufficient number of specimens collectively analysed per tumour type. Given the extent of intra-tumour heterogeneity, we determined the association between TSP-1 and vascularity within individual specimens, based on the average values of TSP-1 and vascularity in 10-20 pre-selected areas per tumour. Read More

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August 2003
8 Reads

Human fibroblasts from normal and malignant breast tissue grown in vitro show a distinct senescence profile and telomerase activity.

Histochem J 2002 Aug-Sep;34(8-9):403-10

Department of Oncology and Haematoncology, PF Calvi Hospital, Noale (Venice), Italy.

The telomerase activity and the senescence profile of cultured breast fibroblasts from normal human interstitial and malignant stromal tissue were studied in comparison with their proliferation and differentiation pattern. Fibroblasts were grown either in the presence or absence of a conditioned medium (CM) obtained from cultures of the oestrogen receptor-positive breast cancer MCF-7 cell line. At different passages (from the 2nd up to the 48th), fibroblasts were examined for the telomerase activity by the Telomerase Repeats Amplification Protocol (TRAP) assay, for proliferation profile by Ki-67 antigen expression, and the myofibroblast or smooth muscle cell-like differentiation pattern by immunofluorescence with monoclonal antibodies specific for smooth muscle markers. Read More

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August 2003
10 Reads

Immunohistochemical characterization of canine intestinal epithelial and mesenchymal tumours with a monoclonal antibody to hepatocyte paraffin 1 (Hep Par 1).

Histochem J 2002 Aug-Sep;34(8-9):397-401

Veterinary Medical Diagnostic Laboratory, University of Missouri, P.O. Box 6023, Columbia, Missouri 65205, USA.

Monoclonal antibody hepatocyte paraffin 1 (Hep Par 1) is reported to be highly specific and sensitive for hepatocellular tumours of humans and dogs. However, in a previous study we observed immunoreactivity for Hep Par 1 in an intestinal adenocarcinoma metastatic to the liver. In this paper, we examined normal intestine and 57 canine intestinal tumours including adenocarcinomas of the small and large intestine, colonorectal polyps, mucinous carcinomas, stromal tumours, leiomyosarcomas, fibrosarcoma, plasmacytomas, and osteosarcoma for immunoreactivity for Hep Par 1. Read More

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August 2003
3 Reads

Large quantity of ribosomal RNA exists extracellularly in mouse spleen.

Histochem J 2002 Aug-Sep;34(8-9):391-6

Department of Immunology, Peking University Health Science Center, Peking University, Beijing 100083, China.

When BALB/c mouse spleens were gently homogenized in saline, the resultant supernatant (without cells and tissue debris) contained significant amount of 28S and 18S ribosomal RNA, reaching up to 70% of the total spleen RNA. Haemoglobin assays indicated that less than 15% of the spleen cells were lysed during the homogenization process, indicating that the majority of the spleen 'supernatant RNA' was from the extracellular space of the organ rather than released by the splenocytes as a consequence of grinding. Quantitative RNA analysis showed that the ratio of spleen supernatant RNA/total RNA of BALB/c mice was inversely correlated with age (from approximately 70% at 3 weeks to 45% at 6 months), but that of BXSB mice (an animal model for systemic lupus erythematosus) remained at about 70% irrespective of age. Read More

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August 2003
2 Reads

Detection of transforming growth factor-alpha and epidermal growth factor receptor mRNA and immunohistochemical localization of their proteins in the ovine uterus during the early implantation period.

Histochem J 2002 Aug-Sep;34(8-9):383-90

Laboratory of Theriogenology, Course of Veterinary Science, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan.

Accumulated evidence suggests that growth factors of the epidermal growth factor (EGF) family play an important role in the murine implantation process. In the sheep, however, the uterine distribution of these factors and their receptor, EGF receptor (EGF-R), during implantation is not known. This study examined the presence of mRNA transcripts and immunohistochemical localization for transforming growth factor-alpha (TGF-alpha), the potent EGF-family member, and EGF-R in the ovine uterus during the early implantation period. Read More

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August 2003
6 Reads

Assembly, stability and integrity of basement membranes in vivo.

Histochem J 2002 Aug-Sep;34(8-9):369-81

Department of Histology, University of Göttingen, Kreuzbergring 36, D-37075, Göttingen, Germany.

Basement membranes are layered structures of the extracellular matrix which separate cells of various kinds from the surrounding stroma. One of the frequently recurring questions about basement membranes is how these structures are formed in vivo. Up to a few years ago, it was thought that basement membranes were formed spontaneously by a process of self-assembly of their components. Read More

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August 2003
5 Reads

In-situ analysis of cellular poly(ADP-ribose) production in scrapie-infected mouse neuroblastoma cells.

Histochem J 2002 Jun-Jul;34(6-7):357-63

School of Clinical Medical Sciences-Gerontology, Institute for Ageing and Health, University of Newcastle upon Tyne, Wolfson Research Centre, NGH, Westgate Road, Newcastle-upon-Tyne NE4 6BE, UK.

Transmissible spongiform encephalopathies (TSEs), also called prion diseases, are characterized by formation of the disease-associated isoform of prion protein (PrP(Sc)), which arises from a normal isoform termed PrP(c) by a post-translational conversion process occurring in an autocatalytic fashion. Oxidative stress has been proposed as a pathogenetic mechanism in TSEs and increased lipid peroxidation has recently been described in prion-infected cell cultures, suggesting an intrinsic link between the presence of prions and oxidative stress. We investigated if poly(ADP-ribose) formation can be detected in cultured cells upon prion infection, as this NAD+-consuming and DNA strand break-activated nuclear enzymatic reaction has the potential to cause rapid and lethal NAD+ depletion in cells under severe oxidative stress. Read More

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January 2004
5 Reads

The nitric oxide synthase-1 and nitric oxide synthase-3/nitric oxide signalling systems in the heart of wild type mice and mouse mutants.

Histochem J 2002 Jun-Jul;34(6-7):345-55

Department of Anatomy II, University Clinic Benjamin Franklin, Free University of Berlin, Königin-Luise-Str. 15, D-14195 Berlin, Germany.

Recently, we have shown that nitric oxide synthase-1 (NOS-1) and thus its product NO are present in the sarcolemma region of a subpopulation of atrial cardiomyocytes in the rat heart. In order to find out whether this newly discovered sarcolemma-associated NOS/NO system represents a general signalling mechanism in the murine rodent heart and whether its properties are comparable to those in skeletal muscle fibres, immunohistochemical and catalytic histochemical methods (including image analysis) were applied to the heart and extensor digitorum longus (EDL) and tongue muscles of wild type and mutant mice. In different strains of wild type mice and NOS-3 knockouts, urea-resistant (and therefore specific) NOS NADPH diaphorase histochemistry and NOS-1 immunohistochemistry revealed that NOS-1 activity and protein were present in the sarcolemma region of a subpopulation of atrial and ventricular working cardiomyocytes, but not in those of the impulse conducting system. Read More

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January 2004
6 Reads

Heparin-binding EGF-like growth factor is seen on the extracellular surface of uterine epithelial cells only after the initial stages of blastocyst attachment.

Histochem J 2002 Jun-Jul;34(6-7):339-43

Department of Anatomy & Histology, F13, The University of Sydney, NSW 2006, Australia.

The presence and distribution of heparin-binding epidermal growth factor in rat uterine epithelial cells was determined immunohistochemically and localized ultrastructurally. Rat uterine tissue was examined on days 1, 3, 6 and 8 of pregnancy and it was found that while presence of this growth factor was evident from day 1, spatial reorganization occurred by the time of blastocyst implantation. Strong apical staining was evident from day 6 to day 8, day 6 being the approximate time of blastocyst implantation. Read More

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January 2004
5 Reads

Expression of aquaporin 3 and its localization in normal skeletal myofibres.

Histochem J 2002 Jun-Jul;34(6-7):331-7

Department of Neurology, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-Ku, Yokohama 227-8501, Japan.

The question whether aquaporin 3 (AQP3) is expressed in normal human skeletal muscle at mRNA and protein levels has been examined, since AQP3 has been reported to be coexpressed with AQP4 in various kinds of tissues other than skeletal muscle. The gel electrophoresis of the reverse transcription polymerase chain reaction (RT-PCR) product of total RNA samples extracted from normal human muscle specimens by using the oligonucleotide primers for AQP3 contained a band of 629 base pairs which corresponded to the base pair length between two primers of AQP3. The nucleotide sequence of this RT-PCR product coincided with that of AQP3. Read More

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January 2004
4 Reads

The beta1 integrin subunit is not a specific component of the costamere domain in human myocardial cells.

Histochem J 2002 Jun-Jul;34(6-7):323-9

Department of Anatomy and Cell Biology, Experimental Cardiology Unit, University of Bergen, 5021 Bergen, Norway.

Studies on altered integrin receptor expression during cardiac hypertrophy and heart failure requires accurate knowledge of the distributional pattern of integrins in myocardial cells. At present the general consensus is that in cardiac muscle the beta1 integrin receptor is mainly localized to the same sarcolemmal domain as vinculin at Z-band levels ('costamere'). Since most previous studies have been focusing on myocardial integrin distribution in lower mammals, the myocardial localization of the beta1 integrin subunit was investigated in biopsies collected from the auricle of patients undergoing a coronary bypass operation. Read More

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January 2004
2 Reads

Immunohistochemical localization of human kallikreins 6 and 10 in pancreatic islets.

Histochem J 2002 Jun-Jul;34(6-7):313-22

Department of Pathology, Evangelismos Hospital, Athens, Greece.

Tissue kallikreins are thought to be present in the pancreatic islets of Langerhans and to aid in the conversion of proinsulin to insulin. In recent immunohistochemical studies, we observed strong staining of the newly identified human kallikreins 6 and 10 (hK6 and hK10) in the islets of Langerhans. Here, we examine hK6 and hK10 immunoexpression in different types of islet cells of the endocrine pancreas, in order to obtain clues for hK6 and hK10 function in these cells. Read More

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January 2004
3 Reads

Small heat shock proteins expression in rat kidneys treated with cyclosporine A alone and combined with melatonin.

Histochem J 2002 Jun-Jul;34(6-7):305-12

Division of Human Anatomy, Department of Biomedical Sciences and Biotechnology, University of Brescia, Viale Europa 11, 25123 Brescia, Italy.

Small heat shock proteins (sHSPs) are cytoskeletal chaperones constitutively expressed in the normal kidney but enhanced with beneficial roles during adverse stimuli. Cyclosporine A is an immunosuppressive drug with major adverse side effect such as severe nephrotoxicity. Among possible mechanisms of cyclosporine A-induced renal damage, oxidative stress and cytoskeletal damage have been suggested. Read More

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January 2004
5 Reads

Pyronin Y as a fluorescent stain for paraffin sections.

Histochem J 2002 Jun-Jul;34(6-7):299-303

Department of Immunology, Peking University Health Science Center, Peking University, Beijing 100083, China.

Pyronin Y has long been used, in combination with other dyes such as Methyl Green, as a differential stain for nucleic acids in paraffin tissue sections. It also forms fluorescent complexes with double-stranded nucleic acids, especially RNA, enabling semi-quantitative analysis of cellular RNA in flow cytometry. However, the possibility of using pyronin Y as a fluorescent stain for paraffin tissue sections has rarely been investigated. Read More

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January 2004
5 Reads

Temporal and spatial expression of fibroblast growth factor receptor 4 isoforms in murine tissues.

Histochem J 2002 Jun-Jul;34(6-7):291-7

School of Biomedical Sciences, University of Queensland, Brisbane, QLD 4072, Australia.

Fibroblast growth factor receptors (FGFRs) undergo highly regulated spatial and temporal changes of expression during development. This study describes the use of quantitative reverse transcriptase-polymerase chain reaction and immunochemistry to assess the changes in expression of FGFR4 as compared to its FGFR4-17a and -17b isoforms in mouse tissues, from early embryogenesis through to adulthood. Compared to FGFR4, the expression of the isoforms is more restricted at all developmental stages tested. Read More

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January 2004
4 Reads

Heterogeneity in olfactory neurons in mouse revealed by differential expression of glycoconjugates.

Histochem J 2002 Jun-Jul;34(6-7):281-9

Department of Anatomy and Developmental Biology, School of Biomedical Sciences, The University of Queensland, Brisbane 4072, Australia.

Cell surface glycoconjugates have been implicated in the growth and guidance of subpopulations of primary olfactory axons. While subpopulations of primary olfactory neurons have been identified by differential expression of carbohydrates in the rat there are few reports of similar subpopulations in the mouse. We have examined the spatiotemporal expression pattern of glycoconjugates recognized by the lectin from Wisteria floribunda (WFA) in the mouse olfactory system. Read More

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January 2004
2 Reads

Subcellular distribution of cadmium and its cellular ligands in mussel digestive gland cells as revealed by combined autometallography and X-ray microprobe analysis.

Histochem J 2002 Jun-Jul;34(6-7):273-80

Cell Biology and Histology Laboratory, Zoology and Animal Cell Dynamics Department, School of Sciences, University of the Basque Country, Bilbao, Basque Country.

Autometallography (AMG) and electron probe X-ray microanalysis (EPXMA) were applied in combination to determine the subcellular distribution of Cd and its subcellular ligands in the digestive gland cells of Cd-exposed mussels Mytilus galloprovincialis. Black silver deposits (BSD), which reveal the presence of metals when AMG is applied, were only localized in digestive cell lysosomes. Digestive cell cytoplasm and basophilic cells were devoid of BSD. Read More

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January 2004
5 Reads

Differential expression of connexin43 in foetal, adult and tumour-associated human brain endothelial cells.

Histochem J 2002 Jun-Jul;34(6-7):265-71

Department of Human Anatomy and Histology, University of Bari Medical School, Polyclinics, piazza Giulio Cesare, 70124 Bari, Italy.

Connexin43 (Cx43), the main protein constituting the gap junctions between astrocytes, has previously been demonstrated in endothelial cells of somatic vessels where the intercellular coupling that it provides plays a role in endothelial proliferation and migration. In this study, Cx43 expression was analysed in human brain microvascular endothelial cells of the cortical plate of 18-week foetal telencephalon, in adult cerebral cortex and glioma (astrocytomas). The study was carried out by immunocytochemistry utilizing a Cx43 monoclonal antibody and a polyclonal antibody anti-GLUT1 (glucose transporter isoform 1) to identify the endothelial cells and to localize Cx43. Read More

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January 2004
4 Reads

In situ localization and in vitro expression of osteoblast/osteocyte factor 45 mRNA during bone cell differentiation.

Histochem J 2002 May;34(5):255-63

Department of Periodontology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-8310, Japan.

The in situ localization of osteoblast/osteocyte factor 45 (OF45) mRNA during bone formation has been examined in the rat mandible from embryonic day 14 (E14) up to postnatal 90-day-old Wistar rats. Gene expression was also examined during cell culture not only in primary rat osteoblast-like cells but also in two clonal rat osteoblastic cell lines with different stages of differentiation, ROB-C26 (C26) and ROB-C20 (C20) using Northern blot analysis. The C26 cell is a potential osteoblast precursor cell line, whereas the C20 cell is a more differentiated osteoblastic cell line. Read More

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May 2002
21 Reads

Analysis of binding of mannosides in relation to Langerin (CD207) in Langerhans cells of normal and transformed epithelia.

Histochem J 2002 May;34(5):247-53

Institute of Anatomy, Department of Otorhinolaryngology, Head and Neck Surgery, 1st Faculty of Medicine, Charles University, Prague, Czech Republic.

Tandem-repeat C-type lectins (pattern-recognition receptors) with specificity for mannosides are intimately involved in antigen recognition, uptake, routing and presentation in macrophages and dendritic cells. In Langerhans cells, Langerin (CD207), a type-II transmembrane protein with a single C-type carbohydrate recognition domain attached to a heptad repeat in the neck region, which is likely to establish oligomers with an alpha-coiled-coil stalk, has been implicated in endocytosis and the formation of Birbeck granules. The structure of Langerin harbours essential motifs for Ca2+-binding and sugar accommodation. Read More

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http://link.springer.com/content/pdf/10.1023/A:1021793530802
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May 2002
22 Reads

Preproenkephalin-like immunoreactive and calcium-binding proteins-like immunoreactive double-labelled neurons in the spinal trigeminal nucleus caudalis of the rat.

Histochem J 2002 May;34(5):241-5

Department of Anatomy and K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, People's Republic of China.

Immunofluorescence histochemical double-staining for preproenkephalin (PPE) and calbindin-D28k (CB), calretinin (CR) or parvalbumin (PV) were performed in the spinal trigeminal nucleus caudalis (Vc) of the rat. Neuronal cell bodies exhibiting PPE-like immunoreactivity were present in all laminae of the Vc, with a higher concentration in lamina II. Most of the CB-, CR- and PV-like immunoreactive neurons were located in lamina II, and some of them were also found in laminae I and III of the Vc. Read More

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May 2002
3 Reads

Visualization of the uptake of high-density lipoprotein by rat aortic endothelial cells and smooth muscle cells in vitro.

Histochem J 2002 May;34(5):233-9

Department of Biology and Life Science Research Center, Tunghai University, Taichung, Taiwan, Republic of China.

High-density lipoproteins (HDL) were conjugated to Fluorescein 1,1'-dioctadecyl 3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) or colloidal gold for the investigation of ultrastructural aspects of binding and uptake of HDL by cholesterol-loaded cultured endothelial and smooth muscle cells from rat aorta. When cells were incubated for 2 h at 4 degrees C, HDL-DiI and HDL-gold conjugates were seen only on the cell surface. When cells were returned to incubation at 37 degrees C for 5 min, HDL-DiI appeared in the cytoplasm and colocalized with the fluorescent cholesteryl ester tag BODIPY-FL-C12. Read More

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May 2002
5 Reads

The expression of Ras-GTPase activating protein SH3 domain-binding proteins, G3BPs, in human breast cancers.

Histochem J 2002 May;34(5):223-31

Institute for Molecular Bioscience, University of Queensland, St. Lucia, 4072, Australia.

Ras-GTPase activating protein SH3 domain-binding proteins 1 and 2 (G3BP1 and G3BP2) have recently been reported to be encoded by two separate genes on human chromosomes 5 and 4 respectively and have been implicated in Ras signalling, NFkappaB signalling, the ubiquitin proteosome pathway and RNA processing. In addition, G3BP1 has recently been implicated in cancer biology. The transcripts for these genes have been shown to be universally expressed; however, this is not the case for the proteins which appear to be tissue and cell type specific. Read More

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May 2002
4 Reads

Improvement of supersensitive immunohistochemistry with an autostainer: a simplified catalysed signal amplification system.

Histochem J 2002 May;34(5):215-22

Second Department of Anatomy, Kagoshima University Faculty of Medicine, Kagoshima, Japan.

The ImmunoMax/catalysed signal amplification (CSA) system is a supersensitive method of paraffin immunohistochemistry. It incorporates antigen retrieval, the streptavidin-biotin complex (sABC) method, and the catalysing reporter deposition/catalysing biotinylated tyramide reaction. Strong, non-specific cytoplasmic reaction in the ImmunoMax/CSA is due to endogenous biotin unmasked in the antigen retrieval step. Read More

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May 2002
23 Reads

A role for small GTPase RhoA in regulating intracellular membrane traffic of lysosomes in invasive rat hepatoma cells.

Histochem J 2002 May;34(5):189-213

Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.

Small GTPase RhoA regulates signal transduction from receptors in the membrane to a variety of cellular events related to cell morphology, motility, cytoskeletal dynamics, cytokinesis, and tumour progression, but it is unclear how RhoA regulates intracellular membrane dynamics of lysosomes. We showed previously by confocal immunofluorescence microscopy that the transfection of dominant active RhoA in MM1 cells causes the dispersal translocation of lysosomes stained for cathepsin D throughout the cytoplasm. Y-27632, a selective inhibitor of p160ROCK, impeded the cellular redistribution of lysosomes and promoted reclustering of lysosomes toward the perinuclear region. Read More

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May 2002
27 Reads

Co-localisation of nitric oxide synthase and endothelin in the rat supraoptic nucleus.

Histochem J 2002 Mar-Apr;34(3-4):181-7

Department of Anatomy and Developmental Biology and Centre for Neuroscience, University College London, Gower Street, London WCIE 6BT, UK.

The co-localisation of neuronal nitric oxide synthase and endothelin-1 was studied in the rat supraoptic nucleus at the electron microscopy level. Double pre-embedding immunocytochemistry was performed using ExtrAvidin-horseradish peroxidase and immunogold-silver techniques. Immunoreactivities to neuronal nitric oxide synthase and endothelin-1 were co-localised in sub-populations of endocrine neurones (cell bodies) and dendrites. Read More

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May 2003
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2 Citations

Endocrine cells in gastric carcinoma and adjacent mucosa. An immunohistochemical and ultrastructural study.

Histochem J 2002 Mar-Apr;34(3-4):173-80

Department of Pathology, Medical Faculty, Trakia University, Armeiska str. 11, BG-6003, Stara Zagora, Bulgaria.

Endocrine cells are often found in human gastric carcinoma and may be recognized by the immunoreactivity of their chromogranin A, peptides and biogenic amines content. Anti-chromogranin A was used to investigate the morphology of endocrine cells using light and electron microscope immunohistochemical techniques. The hormone content of endocrine cells was examined in both tumour tissue and tumour-adjacent mucosa. Read More

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May 2003
5 Reads

Id-1 expression defines a subset of vimentin/S-100beta-positive, GFAP-negative astrocytes in the adult rat pineal gland.

Histochem J 2002 Mar-Apr;34(3-4):167-71

School of Biosciences, Cardiff University, Cardiff CF10 3US, UK.

Id proteins are dominant negative members of the helix-loop-helix (HLH) transcription factor family which are involved in the differentation of many cell types, including glia. We have recently identified the adult rat pineal gland as a major site of Id-1 and Id-3 expression. In the present study, double fluorescence immunocytochemical analysis was used to examine the co-localization of Id-1 and Id-3 with both neuronal (synaptophysin, betaIII-tubulin) and astrocytic markers (GFAP, vimentin, S-100beta) in the rat pineal. Read More

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May 2003
4 Reads

Expression of apoptosis-inducing factor during early neural differentiation in the chick embryo.

Histochem J 2002 Mar-Apr;34(3-4):161-6

Department of Physiology, University of Alberta, Edmonton, Alberta, Canada T6G 1Y6.

The distribution of apoptosis-inducing factor (AIF) immunoreactivity has been studied in the developing somites and nervous system of the chick embryo at embryonic day 4. AIF was found to be expressed primarily in the cytoplasm of cells of the ventral motor roots, at the points of their insertion into the neural tube. Co-localization of mitochondrial AIF immunoreactivity with the epitopes recognized by the monoclonal antibodies HNK-1 and 1E8 suggests that the AIF may be present in Schwann cell precursors as well as in nerve fibres. Read More

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May 2003
3 Reads