45,789 results match your criteria Histochemical Journal[Journal]


Effect of topical ozonetherapy on gingival wound healing in pigs: histological and immuno-histochemical analysis.

J Appl Oral Sci 2018 Dec 10;27:e20180015. Epub 2018 Dec 10.

Gazi University, Faculty of Dentistry, Department of Oral Pathology, Ankara, Turkey.

Objectives: In this study, the effects of ozonetherapy on secondary wound healing were evaluated histologically and immuno-histochemically.

Material And Methods: Material and Methods: 8 healthy pigs were used in this study. Six wounds with 10 mm in diameter were created through the punch technique on the palatinal gingiva of each pig. Read More

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December 2018

EFFECTS OF RENAL DYSFUNCTION ON HEALING OF COLONIC ANASTOMOSIS: EXPERIMENTAL STUDY IN WISTAR RATS.

Arq Bras Cir Dig 2018 Dec 6;31(4):e1398. Epub 2018 Dec 6.

Program of Post-Graduation in Surgical Clinic of the Federal University of Paraná, Curitiba, PR, Brazil.

Background: Chronic kidney disease affects more than 500 million people worldwide. In this context, the uremic toxins present are related to worsening in tissue healing.

Aim: Evaluate on healing of colonic anastomosis in uremic rats, serum and anatomopathological indicators, which may be related to the change tissue repair process. Read More

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December 2018

Histological and histochemical characterisation of glands associated with the feeding appendages of Argulus foliaceus (Linnaeus, 1758).

Parasitol Int 2018 Dec 8. Epub 2018 Dec 8.

Institute of Aquaculture, University of Stirling, Stirling, UK.

Argulus foliaceus (Linnaeus, 1758) is a member of the branchiuran family Argulidae, a group comprising parasitic "fish lice". A. foliaceus is distributed worldwide and causes major economic impacts for cultured freshwater fish globally. Read More

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December 2018
1 Read

The role of the testa during the establishment of physical dormancy in the pea seed.

Ann Bot 2018 Dec 8. Epub 2018 Dec 8.

Department of Experimental Plant Biology, Charles University, Prague, Czech Republic.

Background: A water-impermeable testa acts as a barrier to a seed's imbibition, thereby imposing dormancy. The physical and functional properties of the macrosclereids are thought to be critical determinants of dormancy; however, the mechanisms underlying the maintenance of and release from dormancy in pea are not well understood.

Methods: Seeds of six pea accessions of contrasting dormancy type were tested for their ability to imbibe and the permeability of their testa was evaluated. Read More

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December 2018

Comprehensive Review of Spinal Neurenteric Cysts with a Focus on Histopathological Findings.

Cureus 2018 Sep 28;10(9):e3379. Epub 2018 Sep 28.

Neurosurgery, Seattle Science Foundation, Seattle, USA.

Among the occult spinal dysraphisms, neurenteric cysts (NECs) are rare and are thought to arise due to a failure of the separation of the primitive endoderm and ectoderm. Patients experience various neurological symptoms depending on the location of the lesion. As the epithelial morphology of NECs share similarities with other intracranial and intraspinal cystic growths, the definitive diagnosis of NEC can be made after a histochemical analysis with endodermal markers. Read More

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September 2018

Assessment of the Inhibitory Effects of Ficin-hydrolyzed Gelatin Derived from Squid (Uroteuthis duvauceli) on Breast Cancer Cell Lines and Animal Model.

Iran J Allergy Asthma Immunol 2018 Oct 14;17(5):436-452. Epub 2018 Oct 14.

Department of Marine Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Marine novel natural products have been applied for cancer therapy. Enzyme-digested gelatin hydrolysates have proven to serve as promising sources of potent biologically active peptides. Potential anti-breast cancer properties of the extracted Ficin-digesterd gelatin hydrolysate from Indian squid (Uroteuthis duvauceli) was extensively characterized by cellular and animal models. Read More

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October 2018

Keratinization and mucogenesis in the epidermis of an angler catfish Chaca chaca (Siluriformes, Chacidae): A Histochemical and fluorescence microscope investigation.

Zoology (Jena) 2018 Dec 11;131:10-19. Epub 2018 Oct 11.

Skin Physiology Laboratory, Centre of Advanced Study, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi 221 005, Uttar Pradesh, India. Electronic address:

The present study describes keratinization and mucogenesis in the epidermis of an angler catfish Chaca chaca, using a series of immunochemical, fluorescence and histochemical methods. The epidermis is primarily mucogenic and shows characteristic specialised structures at irregular intervals. These structures are identified keratinized in nature. Read More

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December 2018

Intra-tracheal gene delivery of aerosolized SERCA2a to the lung suppresses ventricular arrhythmias in a model of pulmonary arterial hypertension.

J Mol Cell Cardiol 2018 Nov 28;127:20-30. Epub 2018 Nov 28.

Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, NY, New York, USA. Electronic address:

Background: Pulmonary arterial hypertension (PAH) results in right ventricular (RV) failure, electro-mechanical dysfunction and heightened risk of sudden cardiac death (SCD), although exact mechanisms and predisposing factors remain unclear. Because impaired chronotropic response to exercise is a strong predictor of early mortality in patients with PAH, we hypothesized that progressive elevation in heart rate can unmask ventricular tachyarrhythmias (VT) in a rodent model of monocrotaline (MCT)-induced PAH. We further hypothesized that intra-tracheal gene delivery of aerosolized AAV1. Read More

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November 2018
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Encapsulation of bone marrow-MSCs in PRP-derived fibrin microbeads and preliminary evaluation in a volumetric muscle loss injury rat model: modular muscle tissue engineering.

Artif Cells Nanomed Biotechnol 2018 Dec 4:1-12. Epub 2018 Dec 4.

a Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory , Ankara University Faculty of Science, and Ankara University Stem Cell Institute , Ankara , Turkey.

Repair of volumetric muscle loss (VML) injuries is a complicated endeavour which necessitates the collaborative use of different regenerative approaches and technologies. Herein is proposed the development of fibrin-based microbeads (FMs) alone or as a bone marrow mesenchymal stem cell (MSC) encapsulation matrix for modular muscle engineering. FMs were generated through the ionotropic gelation of alginate and fibrinogen obtained from the platelet-rich plasma of whole blood, and then removing the alginate by citrate treatment. Read More

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December 2018
1 Read

Adrenalectomy for solitary recurrent hepatocellular carcinoma five years after living donor liver transplantation: A case report.

Int J Surg Case Rep 2018 Nov 27;54:23-27. Epub 2018 Nov 27.

Diagnostic & Interventional Radiology Department, Gastrointestinal Surgery Center, College of Medicine, Mansoura University, Egypt.

Introduction: The adrenal gland is a rare site for hepatocellular carcinoma (HCC) recurrence after living-donor liver transplantation (LDLT). Solitary adrenal recurrence can be managed by surgical excision, with expected better survival outcomes. We describe a rare case of successful left adrenalectomy of solitary recurrent HCC in the left adrenal gland 5 years after LDLT. Read More

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November 2018
3 Reads

High thoracic sympathetic block improves coronary microcirculation disturbance in rats with chronic heart failure.

Microvasc Res 2018 Dec 1;122:94-100. Epub 2018 Dec 1.

Department of Cardiac Ultrasound, the First Affiliated Hospital of Harbin Medical University, Harbin, China.

Introduction: Coronary microcirculation disturbance plays an important role in chronic heart failure (CHF). High thoracic sympathetic block (HTSB) is effective to treat CHF, but its impact on coronary microcirculation is unclear.

Methods: Forty male Wistar rats were subcutaneously injected with isoproterenol (340 mg/kg) for 2 days. Read More

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December 2018
1 Read

LncRNA MALAT1 sponges miR-30 to promote osteoblast differentiation of adipose-derived mesenchymal stem cells by promotion of Runx2 expression.

Cell Tissue Res 2018 Dec 3. Epub 2018 Dec 3.

Department of Orthopaedics, Zhongshan Hospital, Fudan University, 180 Fenglin Rd, Shanghai, 200032, People's Republic of China.

Adipose-derived mesenchymal stem cells (ADSCs) are an important source of stem cells for tissue repair and regeneration but the regulatory mechanism of stem cell differentiation is still unclear. Runt-related gene 2 (Runx2) is a bone-specific transcription factor that plays an important role in promoting osteogenic differentiation. Protein levels of Runx2 are regulated by non-coding RNA. Read More

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December 2018

Progressive drought alters architectural and anatomical traits of rice roots.

Rice (N Y) 2018 Dec 4;11(1):62. Epub 2018 Dec 4.

Department of Plant Science, The Pennsylvania State University, 102 Tyson Building, University Park, University Park, PA, 16802-4200, USA.

Background: Root architectural and anatomical phenotypes are important for adaptation to drought. Many rice-growing regions face increasing water scarcity. This study describes drought responses of 11 Egyptian rice cultivars with emphasis on plastic root responses that may enhance drought adaptation. Read More

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December 2018
1 Read

Diagnostic histochemistry in non-neoplastic skin diseases.

Semin Diagn Pathol 2018 Nov 16;35(6):390-398. Epub 2018 Oct 16.

PRW Laboratories, Charlottesville, VA, USA.

Non-neoplastic skin lesions comprise a sizable group of disorders with variable etiologies and clinical manifestations. They can be grouped into vesiculopustular dermatitides; spongiotic and psoriasiform diseases; lichenoid dermatitides; lymphoid infiltrates of the dermis; granulomatous processes; bullous disorders; vasculopathies; panniculitides; deposition disorders; and defects in maintenance of dermal connective tissue. The use of histochemical methods continues to be an indispensable adjunct to conventional microscopy in the further characterization of such lesions. Read More

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November 2018
2 Reads

Divergent Features of Mitochondrial Deficiencies in LGMD2A Associated With Novel Calpain-3 Mutations.

J Neuropathol Exp Neurol 2019 Jan;78(1):88-98

Neuromuscular Diagnostic Laboratory, Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, Lebanon.

Limb girdle muscular dystrophy type 2A (LGMD2A) is an autosomal recessive disorder characterized by progressive muscle weakness and wasting. LGMD2A is caused by mutations in the calpain-3 gene (CAPN3) that encodes a Ca2+-dependent cysteine protease predominantly expressed in the skeletal muscle. Underlying pathological mechanisms have not yet been fully elucidated. Read More

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January 2019
2 Reads

Subcellular accumulation and source of O and HO in submerged plant Hydrilla verticillata (L.f.) Royle under NH-N stress condition.

Aquat Toxicol 2018 Nov 13;207:1-12. Epub 2018 Nov 13.

College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China; Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource, Nanjing Agiricultural University, Nanjing 210095, PR China.

In this study, the effects of excess NH-N on the subcellular accumulation of O and HO in submerged plant Hydrilla verticillata (L.f.) Royle were investigated using both histochemical and cytochemical methods. Read More

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November 2018
2 Reads

Chondroitin sulfate isolated from the secretion of the venom-producing parotoid gland of Brazilian bufonid.

Int J Biol Macromol 2018 Nov 27;124:548-556. Epub 2018 Nov 27.

Laboratório de Histologia Integrativa, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil. Electronic address:

The parotoid gland of bufonids is characterized as a specialized integument region, formed by different gland types. The secretion elaborated by the largest glandular alveoli has been related to animal chemical defense and is constituted by granular protein content, associated with a basophilic and alcianophilic material with features of glycoconjugates. This study aimed to identify and characterize the glycoconjugates in the secretion of the largest granular gland of the parotoid gland of Rinella icterica by histochemical and immunohistochemical techniques at light microscopy, biochemical methods, and nuclear magnetic resonance spectroscopy. Read More

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November 2018

Evaluation of Estrogen and Progesterone Receptors and Her-2 Expression with Grading in the Fine-needle Aspirates of Patients with Breast Carcinoma.

J Cytol 2018 Oct-Dec;35(4):223-228

Department of Pathology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India.

Background: There has been a limited literature with regards to comparison between the pre-operative hormonal/Her-2 neu assessment by immunostaining on fine-needle aspiration (FNA) versus core needle biopsies (CNBs) and their correlation with grading of breast carcinoma.

Materials And Methods: Two hundred fifty FNAs and 201 CNBs from 252 patients with breast carcinoma were subjected immunocytochemical/histochemical (ICC/IHC) staining along with the grading by the Robinson cytologic and modified Scarff-Bloom-Richardson scoring systems, respectively. Depending on the material adequacy, IHC was also performed on cell blocks. Read More

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December 2018

Histochemical localization of N-acetylhexosamine-binding lectin HOL-18 in Halichondria okadai (Japanese black sponge), and its antimicrobial and cytotoxic anticancer effects.

Int J Biol Macromol 2018 Nov 26;124:819-827. Epub 2018 Nov 26.

Laboratory of Glycobiology and Marine Biochemistry, Department of Life and Environmental System Science, Graduate School of NanoBiosciences, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, Japan. Electronic address:

We studied localization and physiological activities of a lectin showing specific binding to N-acetylhexosamines, termed HOL-18, purified from Japanese black sponge (Halichondria okadai). Antiserum against the lectin was generated in rabbit and applied for immunohistochemical analyses. HOL-18 was expressed specifically around water pores and on spicules of sponge tissues. Read More

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November 2018
1 Read
2.858 Impact Factor

Neuroprotection From Organophosphate-Induced Damage by Novel Phenoxyalkyl Pyridinium Oximes in Rat Brain.

Toxicol Sci 2018 Dec;166(2):420-427

Center for Environmental Health Sciences.

The nerve agents are extremely toxic organophosphates which lead to massive inhibition of acetylcholinesterase (AChE) in both the central and peripheral nervous systems. The currently approved pyridinium oxime reactivators of organophosphate-inhibited AChE (eg, 2-PAM in the United States) cannot penetrate the blood-brain barrier because of the permanent positive charge in the pyridinium ring. Therefore these current oximes cannot rescue inhibited AChE in the brain. Read More

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December 2018
2 Reads

Potential protein markers for breast cancer recurrence: a retrospective cohort study.

Cancer Causes Control 2018 Nov 28. Epub 2018 Nov 28.

Markey Cancer Center, University of Kentucky, 744 Rose Street, Combs 206, Lexington, KY, 40536, USA.

Background: We evaluated five key proteins involved in various cancer-related pathways and assessed their relation to breast cancer recurrence.

Methods: We used the Kentucky Cancer Registry to retrospectively identify primary invasive breast cancer cases (n = 475) that were diagnosed and treated at University of Kentucky Medical Center between 2000 and 2007. Breast cancer recurrence was observed in 62 cases during the 5-year follow-up after diagnosis. Read More

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November 2018
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Coryneform Bacteria in Granulomatous Lobular Mastitis: Morphological Diagnosis in Breast Biopsies.

Int J Surg Pathol 2018 Nov 29:1066896918815580. Epub 2018 Nov 29.

1 Pontificia Universidad Católica de Chile, Santiago, Chile.

Granulomatous lobular mastitis is a rare disease whose origin is still unknown and shows an increase in its frequency. Morphological, microbiological, and molecular biology studies have linked this disease to lipophilic and fastidious corynebacteria, suggesting its possible infectious etiology. This series describes and reviews in detail the distinctive morphological characteristics of the bacteria present in the granulomas of this disease, the usefulness of histochemical techniques for their identification, and our proposal for a tissue quantification score for the bacteria. Read More

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November 2018
1 Read
0.961 Impact Factor

[Cyto-inactivation instantly induced by microwave ablation on thyroid nodules].

Zhonghua Yi Xue Za Zhi 2018 Nov;98(43):3524-3527

Super-minimally Invasive Medicals, Shanghai International Medical Center, Shanghai 201210, China.

To explore the effect of microwave ablation on thyroid nodules cell activity by the reaction of key enzyme of cell activation. From November 2017 to February 2018, 104 patients with 120 thyroid nodules underwent ultrasound-guided microwave ablation at Super-minimally Invasive Medicals, Shanghai International Medical Center, aged 14-55 years, 42 males and 62 females.Twice core needle biopsy were performed before and after thermal ablation. Read More

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November 2018
4 Reads

Morphological and biochemical characterization of the cutaneous poison glands in toads ( group) from different environments.

Front Zool 2018 23;15:46. Epub 2018 Nov 23.

1Laboratory of Cell Biology, Instituto Butantan, Av. Vital Brasil 1500, São Paulo, 05503-000 Brazil.

Background: Amphibian defence against predators and microorganisms is directly related to cutaneous glands that produce a huge number of different toxins. These glands are distributed throughout the body but can form accumulations in specific regions. When grouped in low numbers, poison glands form structures similar to warts, quite common in the dorsal skin of bufonids (toads). Read More

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November 2018
2 Reads

The Adenosine Monophosphate (AMP) Analog, 5-Aminoimidazole-4-Carboxamide Ribonucleotide (AICAR) Inhibits Hepatosteatosis and Liver Tumorigenesis in a High-Fat Diet Murine Model Treated with Diethylnitrosamine (DEN).

Med Sci Monit 2018 Nov 26;24:8533-8543. Epub 2018 Nov 26.

Department of Hepatobiliary Surgery, Zhongshan Hospital Xiamen University, Xiamen, Fujian, China (mainland).

BACKGROUND The development and progression of hepatocellular carcinoma (HCC) are associated with obesity and hepatosteatosis. AMP-activated protein kinase (AMPK) regulates metabolic homeostasis. This study aimed to investigate the effects of treatment with the adenosine monophosphate (AMP) analog, 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) on hepatosteatosis in a mouse model fed a high-fat diet (HFD), and on hepatocellular carcinoma (HCC) induced by diethylnitrosamine (DEN) in the HFD mouse model. Read More

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November 2018
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Thymol action on cells and tissues of the synganglia and salivary glands of Rhipicephalus sanguineus sensu lato females (Acari: Ixodidae).

Ticks Tick Borne Dis 2018 Nov 12. Epub 2018 Nov 12.

Departamento de Biologia, Instituto de Biociências, UNESP, Av. 24 A, n° 1515, cx. Postal 199, CEP: 13506-900, Rio Claro, SP, Brazil.

Thymol is a monoterpene present in plants of the families Lamiaceae, Verbenaceae and Apiaceae. Despite its proven acaricidal activity, little is known about the mechanism of action of thymol in ticks. Thus, the aim of this study was to perform a morpho-histochemical analysis of the synganglion and salivary glands of partially engorged females of the brown dog tick, Rhipicephalus sanguineus sensu lato (s. Read More

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November 2018
5 Reads

Diagnostic histochemistry in neuropathology.

Semin Diagn Pathol 2018 Oct 16. Epub 2018 Oct 16.

Division of Neuropathology, Department of Pathology, University of Virginia Medical Center, Charlottesville, VA, United States. Electronic address:

Neuropathologists have a long and storied history of utilizing histochemistry in the characterization and diagnosis of neurological diseases. This review considers stains that are used for the identification of neurons, neuritic processes and axons, myelin sheaths, neuroglial cells, and connective tissue in the nervous system. Rapid histochemical-smear methods for intraoperative diagnosis are also discussed, along with possible roles for lectin-histochemistry in diagnostic neuropathology. Read More

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October 2018

MALDI Imaging for Proteomic Painting of Heterogeneous Tissue Structures.

Proteomics Clin Appl 2018 Nov 24:e1800045. Epub 2018 Nov 24.

Proteopath GmbH, Trier 54296, Germany.

Purpose: To present matrix-assisted laser desorption/ionization (MALDI) imaging as a powerful method to highlight various tissue compartments.

Experimental Design: Formalin-fixed paraffin-embedded (FFPE) tissue of a uterine cervix, a pancreas, a duodenum, a teratoma, and a breast cancer tissue microarray (TMA) are analyzed by MALDI imaging and by immunohistochemistry (IHC). Peptide images are visualized and analyzed using FlexImaging and SCiLS Lab software. Read More

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November 2018
2 Reads

Characterization, recellularization, and transplantation of rat decellularized testis scaffold with bone marrow-derived mesenchymal stem cells.

Stem Cell Res Ther 2018 Nov 21;9(1):324. Epub 2018 Nov 21.

Tissue Engineering Lab, Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Zand St., Shiraz, Fars, 7134845794, Iran.

Background: Regenerative medicine potentially offers the opportunity for curing male infertility. Native extracellular matrix (ECM) creates a reconstruction platform to replace the organs. In this study, we aimed to evaluate the efficiency of the testis decellularized scaffold as a proper niche for stem cell differentiation toward testis-specific cell lineages. Read More

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November 2018
2 Reads

Neuroprotective effects of vitamin D on high fat diet- and palmitic acid-induced enteric neuronal loss in mice.

BMC Gastroenterol 2018 Nov 21;18(1):175. Epub 2018 Nov 21.

Unit of Neurogastroenterology, Department of Experimental Medical Science, Lund University, Sölvegatan 19, BMC B11, 22184, Lund, Sweden.

Background: The role of vitamin D in obesity and diabetes is debated. Obese and/or diabetic patients have elevated levels of free fatty acids, increased susceptibility to gastrointestinal symptoms and are suggested to have altered vitamin D balance. The enteric nervous system is pivotal in regulating gastrointestinal activity and high fat diet (HFD) has been shown to cause loss of enteric neurons in ileum and colon. Read More

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November 2018
1 Read

Slide-free virtual histochemistry (Part II): detection of field cancerization.

Biomed Opt Express 2018 Nov 5;9(11):5253-5268. Epub 2018 Oct 5.

Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Tumor-adjacent "normal" tissue constitutes a peri-tumoral field that affects early cancer detection, risk assessment, surgical decision, and postoperative surveillance. Modern genetic analysis has revealed valuable information from this field, but without the spatial resolution of optical microscopy to understand the vital microenvironments that surround individual cells. Rapidly advanced optical imaging techniques free of labor-intensive sample preparation, despite great promise to perform slide-free imaging of cell and shift the histology-centered cancer diagnostic paradigm, have lacked compatible and complementary histochemical imaging of cell or to interrogate the peri-tumoral field. Read More

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November 2018
2 Reads

Slide-free virtual histochemistry (Part I): development via nonlinear optics.

Biomed Opt Express 2018 Nov 5;9(11):5240-5252. Epub 2018 Oct 5.

Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Histochemistry is a microscopy-based technology widely used to visualize the molecular distribution in biological tissue. Recent developments in label-free optical imaging has demonstrated the potential to replace the conventional histochemical labels/markers (fluorescent antibodies, organic dyes, nucleic acid probes, and other contrast agents) with diverse optical interactions to generate histochemical contrasts, allowing "virtual" histochemistry in three spatial dimensions without preparing a microscope slide (i.e. Read More

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November 2018
2 Reads

Expression of glial cells molecules in the optic nerve of adult dromedary camel (Camelus dromedarius): A histological and immunohistochemical analysis.

Anat Histol Embryol 2018 Nov 18. Epub 2018 Nov 18.

Jessore University of Science and Technology, Jessore, Bangladesh.

The optic nerve (ON) is an important organ in the visual system of animals, which transfers electrical impulses towards the brain from the retina. High enrichment of glial cells in ON is known to support neuron and regulate retinal homoeostasis. However, research on immunohistochemical of glial cells proteins in the camel is scanty in available literature. Read More

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November 2018

Experimental study on the carcinogenic effects of pesticides with ascertained carcinogenic activity under the conditions of its simultaneous influence on the organism of laboratory animals.

Wiad Lek 2018 ;71(7):1274-1280

L.I. Medved's Research Center Of Preventive Toxicology, Food And Chemical Safety, Moh, Ukraine, Kyiv, Ukraine.

Objective: Introduction: It is known that pesticides have both short-term and long-term effects of the action on the human body. Today, taking into account the growth rate of the agricultural crops protection means' market and the expansion of the range of pesticide mixtures and combined formulations, there is a need for a more in-depth study of its possible effects on the environment and the human body. Recently, a new fungicide containing a mixture of two active substances, benthiavalicarb isopropyl and folpet, was introduced for application in Ukraine. Read More

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January 2018
2 Reads

Elevated monocyte-specific type I interferon signalling correlates positively with cardiac healing in myocardial infarct patients but interferon alpha application deteriorates myocardial healing in rats.

Basic Res Cardiol 2018 Nov 12;114(1). Epub 2018 Nov 12.

Department of Cardiology, Amsterdam UMC, University of Amsterdam, Meibergdreef 9, Amsterdam, The Netherlands.

Monocytes are involved in adverse left ventricular (LV) remodelling following myocardial infarction (MI). To provide therapeutic opportunities we aimed to identify gene transcripts in monocytes that relate to post-MI healing and evaluated intervention with the observed gene activity in a rat MI model. In 51 MI patients treated by primary percutaneous coronary intervention (PCI), the change in LV end-diastolic volume index (EDVi) from baseline to 4-month follow-up was assessed using cardiovascular magnetic resonance imaging (CMR). Read More

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November 2018
2 Reads

Proteomic and ecophysiological responses of soybean (Glycine max L.) root nodules to Pb and hg stress.

BMC Plant Biol 2018 Nov 14;18(1):283. Epub 2018 Nov 14.

Proteomics and Bioinformatics Lab, Department of Biotechnology, Jamia Millia Islamia (A Central University), New Delhi, 110025, India.

Background: Lead (Pb) and mercury (Hg) are persistent hazardous metals in industrially polluted soils which can be toxic in low quantities. Metal toxicity can cause changes at cellular and molecular level which should be studied for better understanding of tolerance mechanism in plants. Soybean (Glycine max L. Read More

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November 2018
1 Read

Noninvasive in vivo Assessment of the Re-endothelialization Process Using Ultrasound Biomicroscopy in the Rat Carotid Artery Balloon Injury Model.

J Ultrasound Med 2018 Nov 13. Epub 2018 Nov 13.

Department of Molecular Medicine and Surgery, Karolinska Institute, Stockholm, Sweden.

Objectives: Ultrasound biomicroscopy (UBM), or ultra high-frequency ultrasound, is a technique used to assess the anatomy of small research animals. In this study, UBM was used to assess differences in intimal hyperplasia thickness as a surrogate measurement of the re-endothelialization process after carotid artery balloon injury in rats.

Methods: Ultrasound biomicroscopic data from 3 different experiments and rat strains (Sprague Dawley, Wistar, and diabetic Goto-Kakizaki) were analyzed. Read More

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November 2018
6 Reads

Functional Imaging of Neurotransmitters in Hymenolepis diminuta Treated with Senna Plant Through Light and Confocal Microscopy.

Microsc Microanal 2018 Nov 13:1-10. Epub 2018 Nov 13.

Parasitology Research Laboratory,Department of Zoology,Siksha Bhavana,Visva-Bharati University,Santiniketan 731235,West Bengal,India.

Previous studies have shown the anthelmintic efficacy of Senna alata, Senna alexandrina and Senna occidentalis on the zoonotic parasite Hymenolepis diminuta through microscopic studies on morphological structure. The present study is based on the light and confocal microscopic studies to understand if Senna extracts affect neurotransmitter activity of the parasites. A standard concentration (40 mg/mL) of the three leaf extracts and one set of 0. Read More

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November 2018

Ultrastructural histochemistry in biomedical research: Alive and kicking.

Eur J Histochem 2018 Nov 7;62(4). Epub 2018 Nov 7.

University of Verona, Department of Neurosciences, Biomedicine and Movement Sciences.

The high-resolution images provided by the electron microscopy has constituted a limitless source of information in any research field of life and materials science since the early Thirties of the last century. Browsing the scientific literature, electron microscopy was especially popular from the 1970's to 80's, whereas during the 90's, with the advent of innovative molecular techniques, electron microscopy seemed to be downgraded to a subordinate role, as a merely descriptive technique. Ultrastructural histochemistry was crucial to promote the Renaissance of electron microscopy, when it became evident that a precise localization of molecules in the biological environment was necessary to fully understand their functional role. Read More

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November 2018

Aloperine suppresses allergic airway inflammation through NF-κB, MAPK, and Nrf2/HO-1 signaling pathways in mice.

Int Immunopharmacol 2018 Dec 8;65:571-579. Epub 2018 Nov 8.

Department of Anatomy, Histology and Embryology, Yanbian University Medical College, Yanji 133002, PR China. Electronic address:

To explore the effects of aloperine (ALO) on allergic airway inflammation, we investigated whether its mechanism is related with NF-κB, MAPK, and Nrf2/HO-1 signaling pathways. Histochemical staining and inflammatory cell count were used to observe lung histopathological changes in mice. ELISA was used to detect the content of inflammatory cytokines and IgE in the mouse bronchoalveolar lavage fluid (BALF). Read More

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December 2018
5 Reads

Non-invasive Oxygen-Ozone therapy in treating digital ulcers of patients with systemic sclerosis.

Acta Reumatol Port 2018 Jul-Sep;43(3):210-216

Faculty of Medicine- Suez canal University.

Background: Digital ulcers (DUs) in Systemic sclerosis (SSc) result from recurrent Raynaud's phenomenon (RP) and microtrauma with high impact on quality of life. Medical use of ozone (triatomic oxygen) was initiated in the 19th century. Ozone has multiple therapeutic effects in wound healing due to the property of releasing nascent oxygen, which has been shown to stimulate antioxidant enzymes. Read More

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November 2018
7 Reads

Innervation of the entire internal anal sphincter in a mouse model of Hirschsprung's disease: a first report.

Pediatr Surg Int 2018 Nov 9. Epub 2018 Nov 9.

Department of Pediatric General and Urogenital Surgery, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.

Background: Impaired function of the internal anal sphincter (IAS) may be implicated in postoperative obstructed defecation (POD) that may complicate Hirschsprung's disease (HD) patients. While innervation of part of the IAS in HD has been reported, accurate details based on anatomic landmarks that can explain the clinical morbidity seen in POD are lacking, and there appear to be no studies that specifically document the innervation of the "entire" IAS in HD. We used endothelin receptor-B knockout mice to represent HD (HD-mice) and C57B6 wild mice as controls (C-mice) to investigate the innervation of the entire IAS to assess the pathophysiology of POD experimentally. Read More

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November 2018
3 Reads

Microtubule-associated protein IQ67 DOMAIN5 regulates morphogenesis of leaf pavement cells in Arabidopsis thaliana.

J Exp Bot 2018 Nov 8. Epub 2018 Nov 8.

Department of Molecular Signal Processing, Leibniz Institute of Plant Biochemistry (IPB), Halle (Saale), Germany.

Plant microtubules form a highly dynamic intracellular network with important roles for regulating cell division, cell proliferation and cell morphology. Its organization and dynamics are coordinated by various microtubule-associated proteins (MAPs) that integrate environmental and developmental stimuli to fine-tune and adjust cytoskeletal arrays. IQ67 DOMAIN (IQD) proteins recently emerged as a class of plant-specific MAPs with largely unknown functions. Read More

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November 2018

β-Pinene moderates Cr(VI) phytotoxicity by quenching reactive oxygen species and altering antioxidant machinery in maize.

Environ Sci Pollut Res Int 2018 Nov 7. Epub 2018 Nov 7.

Department of Botany, Panjab University, Chandigarh, 160014, India.

We examined the possible role of monoterpene β-pinene in providing protection against Cr(VI) toxicity in maize (Zea mays). Treatment with β-pinene (10 μM) significantly alleviated Cr(VI) accumulation and recuperated Cr(VI) caused decline in root and coleoptile growth in maize. β-Pinene addition caused a decline in Cr(VI)-induced accumulation of superoxide anion, hydroxyl ion, hydrogen peroxide and confirmed by in-situ detection of ROS using histochemical localization. Read More

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November 2018
2 Reads

Diagnosis and Treatment of Mitochondrial Myopathies.

Neurotherapeutics 2018 Oct;15(4):943-953

Wellcome Centre for Mitochondrial Research, Institute of Neuroscience, Newcastle University, Newcastle upon Tyne, UK.

Mitochondrial myopathies are progressive muscle conditions caused primarily by the impairment of oxidative phosphorylation (OXPHOS) in the mitochondria. This causes a deficit in energy production in the form of adenosine triphosphate (ATP), particularly in skeletal muscle. The diagnosis of mitochondrial myopathy is reliant on the combination of numerous techniques including traditional histochemical, immunohistochemical, and biochemical testing combined with the fast-emerging molecular genetic techniques, namely next-generation sequencing (NGS). Read More

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October 2018
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Lectin histochemical analysis of uterine natural killer cells in normal, hydatidiform molar and invasive molar pregnancy.

Oncol Lett 2018 Nov 20;16(5):6458-6464. Epub 2018 Sep 20.

Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology, Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi 330006, P.R. China.

Uterine natural killer (uNK) cells have been hypothesized to serve a role in controlling trophoblast invasion and proliferation. The aim of the present study was to identify the distribution and number of uNK cells in normal pregnancy (NP), partial mole (PM), complete mole (CM) and invasive mole (IM). uNK cells were detected using dolichos biflorus agglutinin lectin immunohistochemistry in decidual and villous tissues from early NP (n=15), late NP (n=15), PM (n=22), CM (n=20) and IM (n=10). Read More

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November 2018
3 Reads

Amyloid from a histochemical perspective. A review of the structure, properties and types of amyloid, and a proposed staining mechanism for Congo red staining.

Authors:
Richard W Dapson

Biotech Histochem 2018 7;93(8):543-556. Epub 2018 Nov 7.

a Dapson and Dapson LLC , Richland , Michigan , USA.

Amyloid is a diverse group of unrelated peptides or proteins that have positive functionality or are associated with various pathologies. Despite vast differences, all amyloids share several features that together uniquely define the group. 1) All amyloids possess a characteristic cross-ß pattern with X-ray diffraction typical of ß-sheet secondary protein structures. Read More

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November 2018
2 Reads

In vitro axenic germination and cultivation of mixotrophic Pyroloideae (Ericaceae) and their post-germination ontogenetic development.

Ann Bot 2018 Nov 7. Epub 2018 Nov 7.

Department of Experimental Plant Biology, Faculty of Science, Charles University, Viničná, Prague, Czech Republic.

Background And Aims: Pyroloids, forest sub-shrubs of the Ericaceae family, are an important model for their mixotrophic nutrition, which mixes carbon from photosynthesis and from their mycorrhizal fungi. They have medical uses but are difficult to cultivate ex situ; in particular, their dust seeds contain undifferentiated, few-celled embryos, whose germination is normally fully supported by fungal partners. Their germination and early ontogenesis thus remain elusive. Read More

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November 2018
2 Reads

Elemental characterisation of the pyramidal neuron layer within the rat and mouse hippocampus.

Metallomics 2018 Nov 6. Epub 2018 Nov 6.

Molecular and Environmental Sciences Group, Geological Sciences, University of Saskatchewan, 114 Science Place, Saskatoon, Saskatchewan S7N 5E2, Canada and Department of Chemistry, University of Saskatchewan, 110 Science Place, Saskatoon, Saskatchewan S7N 5C9, Canada.

A unique combination of sensitivity, resolution, and penetration make X-ray fluorescence imaging (XFI) ideally suited to investigate trace elemental distributions in the biological context. XFI has gained widespread use as an analytical technique in the biological sciences, and in particular enables exciting new avenues of research in the field of neuroscience. In this study, elemental mapping by XFI was applied to characterise the elemental content within neuronal cell layers of hippocampal sub-regions of mice and rats. Read More

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November 2018
8 Reads

Anti-High Mobility Group Box 1 Antibody Therapy May Prevent Cognitive Dysfunction After Traumatic Brain Injury.

World Neurosurg 2018 Nov 2. Epub 2018 Nov 2.

Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry & Pharmaceutical Sciences, Okayama, Japan. Electronic address:

Background: High mobility group box 1 (HMGB1) protein plays a key role in triggering inflammatory responses in many diseases. Our previous study showed that HMGB1 is found upstream of secondary damage in traumatic brain injury (TBI). We found that anti-HMGB1 monoclonal antibody (mAb) effectively decreased acute brain damage, including the disruption of the blood-brain barrier, brain edema, and neurologic dysfunction. Read More

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November 2018
2 Reads