293,313 results match your criteria Hepatic Carcinoma Primary


Predictive Value of Age-Bilirubin-International Normalized Ratio-Creatinine Score in Short-Term Survival of Acute-on-Chronic Hepatitis B Liver Failure.

Cell Physiol Biochem 2018 11;51(5):2484-2495. Epub 2018 Dec 11.

Department of Hepatic Surgery, The Third Affiliated Hospital, Sun Yat-sen University, Guangdong, China.

Background/aims: The age-bilirubin-international normalized ratio-creatinine (ABIC) score, which is a predictive model commonly used for alcoholic hepatitis, has not yet been studied in acute-on-chronic hepatitis B liver failure (HBV-ACLF). We aimed to investigate the predictive value of the ABIC score in patients with HBV-ACLF.

Methods: This retrospective study involved 398 patients diagnosed with HBV-ACLF, who were divided into a training cohort of 305 patients and a validation cohort of 93 patients. Read More

View Article
December 2018

Resveratrol Ameliorates Lipid Droplet Accumulation in Liver Through a SIRT1/ ATF6-Dependent Mechanism.

Cell Physiol Biochem 2018 11;51(5):2397-2420. Epub 2018 Dec 11.

Research Center for Nutrition and Food Safety, Institute of Military Preventive Medicine, Third Military Medical University, Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing,

Background/aims: Lipid droplets (LDs) are dynamic organelles that store neutral lipids during times of energy excess, and an increased accumulation of LDs in the liver is closely linked to hepatic steatosis. Our previous studies suggested that resveratrol (RSV) supplement could improve hepatic steatosis, but the underlying mechanism, particularly which related to LD accumulation, has not yet been elucidated.

Methods: A high-fat diet (HFD) and palmitic acid were used to induce hepatic steatosis in mouse liver and hepatocytes, respectively. Read More

View Article
December 2018

GLP1 Receptor Agonist Liraglutide Is an Effective Therapeutic Option for Perioperative Glycemic Control in Type 2 Diabetes within Enhanced Recovery After Surgery (ERAS) Protocols.

Eur Surg Res 2018 Dec 11;59(5-6):349-360. Epub 2018 Dec 11.

Background: Enhanced Recovery After Surgery (ERAS) protocols are multimodal perioperative care pathways designed to achieve early recovery after surgical procedures by maintaining preoperative organ function and reducing profound stress responses following surgery [Wilmore and Kehlet: BMJ 2001; 322(7284): 473-6]. Glucagon-like peptide-1 receptor agonists (GLP-1RAgs), such as liraglutide, have recently been widely used as antidiabetic agents in patients with type 2 diabetes (T2D) because they maintain blood glucose at an ideal level throughout the day, including during postprandial periods, thereby improving hypoglycemia and body weight more than insulin therapies. Additionally, the administration of liraglutide may exert cardiovascular, renal, and cerebral protective effects in T2D patients. Read More

View Article
December 2018

In Vivo Assessment of Combined Effects of Glibenclamide and Losartan in Diabetic Rats.

Med Princ Pract 2018 Dec 10. Epub 2018 Dec 10.

Objective: Diabetic complications involve multiple pathological pathways, including hyperglycemia-induced oxidative stress and inflammation. Combination therapy is usually employed to improve treatment outcomes and to lower potential adverse effects. In this study, we evaluated the effects of antidiabetic and antihypertensive agents, glibenclamide (GLI) and losartan (LT), on diabetes-associated metabolic changes in rats. Read More

View Article
December 2018

Recruitment of macrophages and bone marrow stem cells to regenerating liver promoted by sodium phthalhydrazide in mice.

Biomed Pharmacother 2018 Dec 8;110:594-601. Epub 2018 Dec 8.

Institute of Immunopathology and Preventive Medicine, Povsetova ulica 29, 1000 Lublana, Slovenia. Electronic address:

Pharmacological interventions which could be hepatoprotective, depending on bioavailability, anti-inflammatory and macrophage-targeting potential of drugs, are still at early preclinical stages. Existing evidence from many animal models of liver injury, as well as from human data, indicate that pharmacological and/or phytochemical interventions have limited impact on liver recovery. Recent studies on stem cell therapies focused on different cell subsets involved in tissue repair, including monocytes/macrophages and bone marrow cells migrating to the injured liver. Read More

View Article
December 2018
1 Read

Novel hepatoprotective role of Leonurine hydrochloride against experimental non-alcoholic steatohepatitis mediated via AMPK/SREBP1 signaling pathway.

Biomed Pharmacother 2018 Dec 7;110:571-581. Epub 2018 Dec 7.

State Key Laboratory of Natural Medicines, China Pharmaceutical University, 210009, Nanjing, Jiangsu, China; Jiangsu Key Laboratory of Drug Discovery for Metabolic Diseases, China Pharmaceutical University, 210009, Nanjing, Jiangsu, China. Electronic address:

Background And Aims: Non-alcoholic steatohepatitis (NASH) is the hepatic manifestation of metabolic syndrome and is characterized by steatosis, inflammation, and fibrosis. We aim to characterize the hepatoprotective effects of Leonurine hydrochloride (LH) and the possible pathway in a cell and rodent model of diet-induced steatohepatitis (NASH).

Methods: For in vitro studies, Palmitic acid (PA) and free fatty acid (FFA) induced HepG2 and HL7702 steatosis cell models were used. Read More

View Article
December 2018

Immunotoxic effects of 4-nonylphenol on Clarias gariepinus: Cytopathological changes in hepatic melanomacrophages.

Aquat Toxicol 2018 Dec 3;207:83-90. Epub 2018 Dec 3.

Medical Physiology Department, Faculty of Medicine, Assiut University, Assiut, Egypt.

Melanomacrophage centres (MMCs) play a key role in the immune response in fish. They are considered sensitive bio-monitoring structures with roles in the assessment of toxicant impacts. The aim of this study was to examine the potential histopathological effect of 4-nonylphenol (4-NP) on hepatic MMCs in Clarias gariepinus. Read More

View Article
December 2018

Validation of the albumin-indocyanine green evaluation model in patients with resected hepatocellular carcinoma and comparison with the albumin-bilirubin score.

J Hepatobiliary Pancreat Sci 2018 Dec 8. Epub 2018 Dec 8.

Department of General and Oncological Surgery, Mauriziano Hospital, Turin, Italy.

Background: The albumin-indocyanine green evaluation (ALICE) model based on serum albumin and indocyanine retention rate, has been shown to be an effective method for predicting postoperative outcomes in hepatocellular carcinoma patients. Aim of the study was to validate the ALICE model in a large Western cohort of patients by comparing the Albumin-Bilirubin (ALBI) score and Child-Turcotte-Pugh (CTP) score.

Methods: A total of 400 patients who underwent hepatic resection from 01/2005 to 06/2016 at 3 centers were enrolled. Read More

View Article
December 2018

Contrast-enhanced ultrasonography is a feasible technique for quantifying hepatic microvascular perfusion in dogs with extrahepatic congenital portosystemic shunts.

Vet Radiol Ultrasound 2018 Dec 9. Epub 2018 Dec 9.

Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.

Extrahepatic-congenital portosystemic shunt is a vascular anomaly that connects the portal vein to the systemic circulation and leads to a change in hepatic microvascular perfusion. However, an assessment of hepatic microvascular perfusion is limited by conventional diagnostic modalities. The aim of this prospective, exploratory study was to assess hepatic microvascular perfusion in dogs with extrahepatic-congenital portosystemic shunt using contrast-enhanced ultrasonography (CEUS) using perfluorobutane (Sonazoid ). Read More

View Article
December 2018

Multiple receptors converge on H2-Q10 to regulate NK & γδT cell development.

Immunol Cell Biol 2018 Dec 9. Epub 2018 Dec 9.

Department of Immunology and Pathology, Central Clinical School, Monash University, Melbourne, Australia.

Class Ib MHC are an extended family of molecules which demonstrate tissue-specific expression and presentation of monomorphic antigens. These characteristics tend to imbue class Ib MHC with unique functions. H2-Q10 is potentially one such molecule in that it is overexpressed in the liver but its immunological function is not known. Read More

View Article
December 2018

Combination of Whole Grape Seed Flour and Newly Isolated Kefir Lactic Acid Bacteria Reduces High-Fat (HF)-induced Hepatic Steatosis.

Mol Nutr Food Res 2018 Dec 11:e1801040. Epub 2018 Dec 11.

J.-H. Kwon, H. G. Lee, K.-H. Seo, H. Kim, Department of Food and Nutrition, Hanyang University, Seoul.

Scope: This study aimed to determine the separate and combined effect of a prebiotic (Catechin-rich wine grape seed flour, GSF) and a probiotic (newly isolated kefir lactic acid bacteria, LAB) on hepatic steatosis of obese mice.

Methods And Results: High-fat (HF)-induced obese mice were fed HF diet containing 6% microcrystalline cellulose (MCC, control), 10% GSF, orally administrated LAB, or combination of GSF and LAB for 9 weeks. There was a significant reduction of body weight gain, and liver weights, plasma insulin concentrations, and HOMA-IR in all experimental groups compared to control. Read More

View Article
December 2018

Differential regulation of intestinal and hepatic CYP3A by 1α,25-dihydroxyvitamin D : Effects on in vivo oral absorption and disposition of buspirone in rats.

Drug Dev Res 2018 Dec 10. Epub 2018 Dec 10.

Department of Manufacturing Pharmacy, College of Pharmacy, Pusan National University, Busan, South Korea.

1α,25-Dihydroxyvitamin D (also called 1,25(OH) D or calcitriol) is the biologically active form of vitamin D, which functions as a ligand to the vitamin D receptor (VDR). It was previously reported that intestinal cytochrome P450 3A (CYP3A) expression was altered by 1,25(OH) D -mediated VDR activation. However, to clarify whether the change in CYP3A subfamily expression by VDR activation can affect metabolic function, further evidence is needed to prove the effect of 1,25(OH) D treatment on CYP3A-mediated drug metabolism and pharmacokinetics. Read More

View Article
December 2018
1 Read

Preconditioning of umbilical cord-derived mesenchymal stem cells by rapamycin increases cell migration and ameliorates liver ischaemia/reperfusion injury in mice via the CXCR4/CXCL12 axis.

Cell Prolif 2018 Dec 10:e12546. Epub 2018 Dec 10.

Department of Hepatic Surgery and Liver Transplantation Center, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Objectives: Transfusion of umbilical cord-derived mesenchymal stem cells (UC-MSCs) is a novel strategy for treatment of various liver diseases. However, the therapeutic effect of UC-MSCs is limited because only a few UC-MSCs migrate towards the damaged regions. In this study, we observed the effects of autophagy on the migration of UC-MSCs in vitro and in a model of liver ischaemia/reperfusion (I/R) injury. Read More

View Article
December 2018

Lipophagy in nonliver tissues and some related diseases: Pathogenic and therapeutic implications.

J Cell Physiol 2018 Dec 10. Epub 2018 Dec 10.

Department of Basic nursing, Nursing College, University of South China, Hengyang, China.

Lipid autophagy (lipophagy) is defined as a selective autophagy process in which some intracellular lipid droplets are selectively degraded by autophagic lysosomes pathway. The occurrence of lipophagy was first discovered in liver tissues. Additionally, abundant evidence indicated that the occurrence of hepatic lipophagy has been implicated in many liver diseases including fatty liver diseases, nonalcoholic fatty liver diseases, liver fibrosis, and liver cirrhosis. Read More

View Article
December 2018

Management and treatment of the HCV-infected kidney transplant patient.

Semin Dial 2018 Dec 9. Epub 2018 Dec 9.

Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania.

The prevalence of hepatitis C virus infection is increased in patients with end stage kidney disease compared to the general population and is an adverse outcome determinant. Direct-acting antiviral therapy for hepatitis C virus is changing the management paradigm of infected kidney transplant candidates and recipients, with potential to reduce patient morbidity and mortality. This review describes the hepatic and nonhepatic manifestations of hepatitis C virus in kidney transplant patients as well as management and treatment strategies to optimize transplant outcomes, highlighting the importance of direct-acting antivirals in this population. Read More

View Article
December 2018

CCR5 deficiency enhances hepatic innate immune cell recruitment & inflammation in a murine model of acute hepatitis B infection.

Immunol Cell Biol 2018 Dec 8. Epub 2018 Dec 8.

Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Human genetic studies demonstrate a link between the 32-bp deletion that produces a nonfunctional CCR5 receptor (CCR5∆32) and enhanced recovery from acute hepatitis B virus (HBV) infection. To investigate the role of CCR5 in immune responses to acute HBV, we intravenously infected Ccr5 (WT) and Ccr5 (KO) mice with a replication-incompetent adenovirus containing the overlapping HBV1.3 construct (AdHBV), or vector control. Read More

View Article
December 2018

Perilipin-2 promotes obesity and progressive fatty liver disease in mice through mechanistically distinct hepatocyte and extra-hepatocyte actions.

J Physiol 2018 Dec 8. Epub 2018 Dec 8.

Graduate Program in Integrated Physiology, University of Colorado School of Medicine, Aurora, CO.

Key Points: Wild type mice as well as mice with hepatocyte-specific or whole-body deletions of perilipin-2 (Plin2) were used to define hepatocyte and extra-hepatocyte effects of altered cellular lipid storage on obesity and non-alcoholic fatty liver disease (NAFLD) pathophysiology in a western-diet (WD) model of these disorders. Extra-hepatocyte actions of Plin2 are responsible for obesity, adipose inflammation, and glucose clearance abnormalities in WD-fed mice. Hepatocyte and extra-hepatic actions of Plin2 mediate fatty liver formation in WD-fed mice through distinct mechanisms. Read More

View Article
December 2018

Integrative analysis of DNA methylation and gene expression identify a six epigenetic driver signature for predicting prognosis in hepatocellular carcinoma.

J Cell Physiol 2018 Dec 7. Epub 2018 Dec 7.

Department of Surgery, Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

DNA methylation is a crucial regulator of gene transcription in the etiology and pathogenesis of hepatocellular carcinoma (HCC). Thus, it is reasonable to identify DNA methylation-related prognostic markers. Currently, we aimed to make an integrative epigenetic analysis of HCC to identify the effectiveness of epigenetic drivers in predicting prognosis for HCC patients. Read More

View Article
December 2018

Preventive effect of omega-3 fatty acids in a rat model of stress-induced liver injury.

J Cell Physiol 2018 Dec 7. Epub 2018 Dec 7.

Histology and Cell Biology Department, Faculty of Medicine, Minia University, Minia, Egypt.

Omega-3 fatty acids are gaining attention as a therapeutic agent of many diseases. Their protective effect in a variety of diseases has been demonstrated. To the best of our knowledge, this is the first study on omega-3 fatty acids related to acute cold-restraint stress (CRS) induced hepatic dysfunction in rats. Read More

View Article
December 2018

HOTAIR participates in hepatic insulin resistance via regulating SIRT1.

Eur Rev Med Pharmacol Sci 2018 Nov;22(22):7883-7890

Department of Endocrinology, The 6th Affiliated Hospital of Xinjiang Medical University, Urumqi,

Objective: The aim of this study was to investigate the effect of long non-coding RNA (lncRNA) HOTAIR on hepatic insulin resistance and to explore the possible underlying mechanism.

Patients And Methods: Liver tissues of type 2 diabetes mellitus (T2D) patients, healthy controls, C57BL/6J mice fed with a high-fat diet and db/db mice were harvested. Meanwhile, the expressions of HOTAIR and SIRT1 were detected. Read More

View Article
November 2018

Malignant Vascular Tumors of the Liver in Adults.

Semin Liver Dis 2018 Dec 7. Epub 2018 Dec 7.

Department of Radiology, BCLC Group, Hospital Clinic Barcelona, University of Barcelona, Barcelona, Spain.

Hepatic angiosarcoma and epithelioid hemangioendothelioma (EHE) might be clinically considered a spectrum since, although more frequently presenting indolent behavior, EHE occasionally evolves to high-grade neoplasms. However, in most circumstances, pathological and immunohistochemical patterns define this differential diagnosis. More recently, molecular pathways for angiosarcoma and for EHE from other organs and from soft tissue have been proved different, paving the way for future morpho-molecular assessment of their hepatic counterpart. Read More

View Article
December 2018

Efficacy and safety of lanreotide in Korean patients with metastatic, well-differentiated gastroenteropancreatic-neuroendocrine tumors: a retrospective analysis.

Invest New Drugs 2018 Dec 10. Epub 2018 Dec 10.

Department of Oncology, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul, 05505, South Korea.

Lanreotide autogel is a long-acting somatostatin analogue with proven efficacy and safety in patients with well-differentiated (WD) gastroenteropancreatic-neuroendocrine tumors (GEP-NETs) in a prior randomized phase III trial (CLARINET). However, the CLARINET study only enrolled patients with Ki-67 index <10%, and few patients of Asian ethnicity were included. We retrospectively analyzed the efficacy and safety of lanreotide in Korean patients with GEP-NETs in the daily practice setting. Read More

View Article
December 2018
1 Read

A Rodent Model for Cell Transplantation of Hepatic Progenitor Cells.

Methods Mol Biol 2019 ;1905:211-219

Department of Molecular Life Sciences, Tokai University School of Medicine, Isehara, Kanagawa, Japan.

Hepatic progenitor cells are defined as cells exhibiting potency for active proliferation and capacity for bipotential differentiation into hepatocytes and cholangiocytes. To prove the capacity of target cells for terminal differentiation and reconstitution of organs, cell transplantation models have been widely used in previous studies, including those involving the liver. Here we describe a protocol for transplantation of hepatic progenitor cells using retrorsine pretreatment and partial hepatectomy. Read More

View Article
January 2019

Analysis for Remodeling of Hepatic Tissue Structures in 3D During Regeneration.

Authors:
Kota Kaneko

Methods Mol Biol 2019 ;1905:187-198

Department of Pathology, School of Medicine, University of California, San Diego, CA, USA.

It has been demonstrated that the liver remarkably alter its tissue structures during regeneration, and various types of liver stem or progenitor cells locating in specific areas in the liver tissue contribute to regeneration. Therefore, it is important to analyze the dynamic rearrangement of the liver tissue structures in 3D for better understanding the process and mechanism of liver regeneration. Here we describe a macroscopic analysis method to visualize the whole 3D structure of the vasculatures and the biliary tree, which are dynamically remodeled during regeneration, and a microscopic analysis method to visualize detailed structures at the cellular level, which can compensate what cannot be detected in the macroscopic analysis. Read More

View Article
January 2019

Generation of Hepatic Organoids with Biliary Structures.

Methods Mol Biol 2019 ;1905:175-185

Institute of Industrial Science, The University of Tokyo, Tokyo, Japan.

Incorporation of bile drainage system into engineered liver tissue is an important issue to advance liver regenerative medicine. Our group reported that three-dimensional (3D) coculture of fetal liver cells (FLCs) and adult rat biliary epithelial cells (BECs) allows reconstruction of hepatic spheroids that possess bile ductular structures. In this chapter, we describe the detailed protocol to isolate FLCs and BECs and to generate the spheroids with bile drainage system using these two types of primary cells. Read More

View Article
January 2019

Reconstruction of Hepatic Tissue Structures Using Interstitial Flow in a Microfluidic Device.

Authors:
Ryo Sudo

Methods Mol Biol 2019 ;1905:167-174

Department of System Design Engineering, Keio University, Yokohama, Japan.

Construction of three-dimensional (3D) hepatic tissue structures is important for in vitro tissue engineering of the liver, because 3D culture of hepatocytes is critical for the maintenance of liver-specific functions. Although conventional 3D culture methods are useful for constructing 3D hepatic tissue structures, the precise control of culture microenvironments is required to construct more physiological tissues in vitro. Recent advances in microfluidics technologies have allowed us to utilize microfluidic devices for hepatic cell culture, which opened the door for creating more physiological 3D culture models of the liver. Read More

View Article
January 2019
1 Read

Generation of Hepatic Tissue Structures Using Multicellular Spheroid Culture.

Methods Mol Biol 2019 ;1905:157-165

Department of Life and Environmental System Science, Graduate School of Nanobioscience, Yokohama City University, Yokohama, Japan.

The hepatic functions of the hepatocytes in multicellular spheroid (MCS) are lower than those in the liver. One of the causes is that conventional hepatic MCSs do not reproduce liver-specific microstructures such as hepatic cord. It is necessary to design the inner structure of hepatic MCSs mimicking a structural feature of hepatic cord to represent further hepatic functions. Read More

View Article
January 2019

Culture System of Bile Duct-Like Cystic Structures Derived from Human-Inducible Pluripotent Stem Cells.

Methods Mol Biol 2019 ;1905:143-153

Department of Molecular Life Sciences, Tokai University School of Medicine, Isehara, Kanagawa, Japan.

Inducible pluripotent stem (iPS) cells are multipotent stem cells that are produced by gene transfer of reprogramming factors to somatic cells. They are thought to be an important source of regenerative medicine because of their pluripotency and self-renewal ability. Although the liver has high regeneration ability, continuous death of hepatocytes due to chronic inflammation leads to liver cirrhosis and liver carcinoma. Read More

View Article
January 2019

Induction of Functional Hepatocytes from Human iPSCs.

Methods Mol Biol 2019 ;1905:131-142

Laboratory of Cell Growth and Differentiation, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan.

Human-induced pluripotent stem cells (iPSCs) could be a useful source for production of hepatocytes. Here, we develop protocols to generate iPSC-derived liver progenitor cells, liver sinusoidal endothelial cells (LSECs), and hepatic stellate cells (HSCs). We also establish long-term two-dimensional co-culture system to induce functional hepatocytes from iPSC-derived liver cells. Read More

View Article
January 2019

Chemically Induced Liver Progenitors (CLiPs): A Novel Cell Source for Hepatocytes and Biliary Epithelial Cells.

Methods Mol Biol 2019 ;1905:117-130

Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, Tokyo, Japan.

Bipotent liver progenitor cells (LPCs) are promising cell sources for cell transplantation therapy in hepatic disorders as well as biliary dysfunctions. Using a cocktail of small molecules, we recently reported a novel approach to generate bipotent LPCs, named chemically induced liver progenitors (CLiPs), from adult rat hepatocytes. In this chapter, we describe a detailed protocol for the induction of rat CLiPs. Read More

View Article
January 2019

Analysis for the Heterogeneity of Liver Progenitor Cells.

Authors:
Kenji Kamimoto

Methods Mol Biol 2019 ;1905:71-82

Department of Developmental Biology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA.

Recent technological advances have revealed the heterogeneity of cells and tissues. Existence of heterogeneity in hepatic progenitor cells is becoming apparent by various experimental approaches, and here we describe a series of techniques to investigate the proliferative heterogeneity of these cells. We have developed a new technique by combining genetic lineage tracking and three-dimensional imaging methods. Read More

View Article
January 2019

Long-Term Culture of Mouse Fetal Hepatic Stem/Progenitor Cells.

Methods Mol Biol 2019 ;1905:3-8

Division of Gastroenterology and Hepatology, Graduate School of Medical and Dental Science, Niigata University, Niigata, Japan.

Mouse fetal liver includes abundant hepatic stem/progenitor cells (HSPCs). Easy expansion with passage of HSPCs is necessary to obtain steady data. However, it is often difficult to enrich only HSPCs, and HSPCs can die when usual trypsin is used for replating. Read More

View Article
January 2019

Essential Oil from Carpesium abrotanoides L. Induces Apoptosis via Activating Mitochondrial Pathway in Hepatocellular Carcinoma Cells.

Curr Med Sci 2018 Dec 7;38(6):1045-1053. Epub 2018 Dec 7.

School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, 430074, China.

The effects of essential oil from Carpesium abrotanoides L. (CAEO) on the proliferation and apoptosis of human hepatic cancer cells were investigated in this study. MTT assays indicated that CAEO inhibited the proliferation of HCC cells with the IC values ranging from 41. Read More

View Article
December 2018

Pharmacological activation of dimethylarginine dimethylaminohydrolase (DDAH) activity by inorganic nitrate and DDAH inhibition by N-hydroxy-L-arginine, N,N-dimethyl-L-citrulline and N,N-dimethyl-N-hydroxy-L-citrulline: results and overview.

Amino Acids 2018 Dec 8. Epub 2018 Dec 8.

Institute of Toxicology, Core Unit Proteomics, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625, Hannover, Germany.

Asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) are endogenous inhibitors of nitric oxide (NO) synthase. SDMA is excreted in the urine without major metabolization. About 10% of daily produced ADMA are excreted unchanged in the urine. Read More

View Article
December 2018

Impact of chronic kidney dysfunction on serum Sulfatides and its metabolic pathway in mice.

Glycoconj J 2018 Dec 10. Epub 2018 Dec 10.

Department of Metabolic Regulation, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano, 390-8621, Japan.

Serum sulfatides are critical glycosphingolipids that are present in lipoproteins and exert anticoagulant effects. A previous study reported decreased levels of serum sulfatides in hemodialysis patients and suggested an association with cardiovascular disease. However, the mechanism of changes in serum sulfatides in chronic kidney dysfunction has not been well investigated. Read More

View Article
December 2018

Treprostinil reduces endothelial damage in murine sinusoidal obstruction syndrome.

J Mol Med (Berl) 2018 Dec 7. Epub 2018 Dec 7.

Institute of Pharmacology and the Gaston H. Glock Research Laboratories for Exploratory Drug Development, Centre of Physiology and Pharmacology, Medical University of Vienna, Vienna, Austria.

Sinusoidal obstruction syndrome (SOS) is a major complication after hematopoietic stem cell transplantation and belongs to a group of diseases increasingly identified as transplant-related systemic endothelial disease. Administration of defibrotide affords some protection against SOS, but the effect is modest. Hence, there is unmet medical need justifying the preclinical search for alternative approaches. Read More

View Article
December 2018

Roles and mechanisms of action of HNF‑4α in the hepatic differentiation of WB‑F344 cells.

Int J Mol Med 2018 Dec 3. Epub 2018 Dec 3.

Department of General Surgery, Tongji Hospital, Tongji University Medical School, Shanghai 200065, P.R. China, P.R. China.

Hepatocyte nuclear factor 4 α (HNF‑4α) is a nuclear receptor and mediates hepatic genes. WB‑F344 liver epithelial cells can differentiate into hepatocytes. The present study aimed to examine the roles and mechanisms of action of HNF‑4α on the hepatic differentiation of WB‑F344 cells. Read More

View Article
December 2018

α‑lipoic acid protects against carbon tetrachloride‑induced liver cirrhosis through the suppression of the TGF‑β/Smad3 pathway and autophagy.

Mol Med Rep 2018 Dec 4. Epub 2018 Dec 4.

Spleen, Stomach and Hepatobiliary Department, The First Affiliated Hospital, Henan University of Chinese Medicine, Zhengzhou, Henan 450004, P.R. China.

α‑lipoic acid (ALA) is a naturally occurring antioxidant with protective effects against various hepatic injuries. The aim of the present study was to investigate the mechanisms by which ALA protects the liver from carbon tetrachloride (CCl4)‑induced liver cirrhosis. The widely used liver cirrhosis rat model was established via an intraperitoneal injection of 2 mg/kg 50% CCl4, three times/week for 8 weeks. Read More

View Article
December 2018

Repression of liver cirrhosis achieved by inhibitory effect of miR-454 on hepatic stellate cells activation and proliferation via Wnt10a.

J Biochem 2018 Dec 7. Epub 2018 Dec 7.

Department of Hepatopathy, the Second Hospital of Nanjing, Medical School, Southeast University, Nanjing, Jiangsu, China.

Background: As is known, hepatic stellate cells (HSC) activation contributes to liver cirrhosis. This study aims to find out the acting mechanisms of miR-454 inhibiting the activation and proliferation of hepatic stellate cells.

Methods: The expression of Col1A1, α-SMA and Wnt10a were determined by western blot, and the miR-454 level was determined by qRT-PCR in this study. Read More

View Article
December 2018

Identification of Abnormal Fucosylated-Glycans Recognized by LTL in Saliva of HBV-Induced Chronic Hepatitis, Cirrhosis, and Hepatocellular Carcinoma.

Glycobiology 2018 Dec 7. Epub 2018 Dec 7.

Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, China.

The hepatitis B virus (HBV)-induced chronic liver diseases are serious health threats worldwide. There is evidence to display the alterations of salivary N-linked glycans related to the development of HBV-infected liver diseases. Here, we further investigated the alterations of fucosylated N/O-glycans recognized by LTL in saliva from 120 subjects (30 healthy volunteers (HV), 30 patients with hepatitis B (HB), 30 patients with hepatic cirrhosis (HC), and 30 patients with hepatocellular carcinoma (HCC)) using salivary microarrys and MALDI-TOF/TOF-MS. Read More

View Article
December 2018

[Acute pulmonary fibrosis associated with Paraquat poisoning. Report of one case].

Rev Med Chil 2018 Jul;146(7):938-941

Centro de Pacientes Críticos, Clínica Las Condes, Santiago, Chile.

Paraquat, a non-selective bipyridyl pesticide, is one of the leading causes of death from intoxication in many parts of Asia and America. It is the second most sold herbicide worldwide, being widely used in Chile. Its ingestion generates toxicity due to the release of superoxide radicals, mainly affecting kidneys, lungs and liver. Read More

View Article

The importance of the histopathological examination in lethal acute intoxication with ethylene glycol. Case report.

Rom J Morphol Embryol 2018 ;59(3):965-969

Department of Morphological Disciplines, Faculty of Medicine and Pharmacy, University of Oradea, Romania;

Ethylene glycol is a toxic alcohol that is mainly introduced into an organism through the digestive pathway. Its priority toxic metabolites are glycolic acid and oxalic acid. We present the case of a young person, of the male persuasion, without any personal pathological history, found unconscious and presenting signs of violence. Read More

View Article
January 2018

Dietary methionine restriction reduces hepatic steatosis and oxidative stress in high-fat-fed mice by promoting HS production.

Food Funct 2018 Dec 10. Epub 2018 Dec 10.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China. and Center for Food Nutrition and Functional Food Engineering, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.

Methionine is an essential amino acid that plays important roles in mammalian metabolism. Interestingly, previous studies have consistently shown that a methionine-restricted diet (MRD) reduces hepatic lipid levels, reactive oxygen species production, and oxidative stress damage. However, the mechanisms responsible for these beneficial effects are unknown. Read More

View Article
December 2018

Betaine increases mitochondrial content and improves hepatic lipid metabolism.

Food Funct 2018 Dec 10. Epub 2018 Dec 10.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, P.R. China.

The liver plays a critical role in lipid metabolism. Hepatic dysfunction is not only the direct cause of fatty liver disease, but the main risk factor for obesity, diabetes, and other metabolic diseases. So far, therapeutic strategies against fatty liver disease are very limited. Read More

View Article
December 2018

Hypercoagulability in End-stage Liver Disease: Review of Epidemiology, Etiology, and Management.

Transplant Direct 2018 Nov 26;4(11):e403. Epub 2018 Oct 26.

Department of Anesthesiology and Perioperative Medicine, The Pennsylvania State University-Milton S. Hershey Medical Center, Hershey, PA.

In this review, we analyze the epidemiology of thromboses related to end-stage liver disease (ESLD), discuss causes of hypercoagulability, describe susceptible populations, and critically evaluate proposed prophylaxis and treatment of thromboses. Classically, ESLD has been regarded as a model for coagulopathy, and patients were deemed to be at high risk for bleeding complications. Patients with ESLD are not auto-anticoagulated, and they do not have a lower risk of portal vein thrombosis, intracardiac thrombus formation, pulmonary embolism or hepatic artery thrombosis. Read More

View Article
November 2018

A Comparative Study of Single and Dual Perfusion During End-ischemic Subnormothermic Liver Machine Preservation.

Transplant Direct 2018 Nov 23;4(11):e400. Epub 2018 Oct 23.

Division of Organ Transplantation, Department of Surgery, UMass Memorial Medical Center, University of Massachusetts, Worcester, MA.

Background: It remains controversial if arterial perfusion in addition to portal vein perfusion during machine preservation improves liver graft quality. Comparative studies using both techniques are lacking. We studied the impact of using single or dual machine perfusion of donation after circulatory death rat livers. Read More

View Article
November 2018

The Effectiveness of Hepatic Arterial Infusion Chemotherapy with 5-Fluorouracil/Cisplatin and Systemic Chemotherapy with Ramucirumab in Alpha-Fetoprotein-Producing Gastric Cancer with Multiple Liver Metastases.

Case Rep Oncol Med 2018 11;2018:5402313. Epub 2018 Nov 11.

Department of Pathology, Kurume University Hospital, Kurume, Japan.

Alpha-fetoprotein- (AFP-) producing gastric cancer (AFPGC) is characterized by a high incidence of liver and lymph node metastases and poor prognosis. Although several case reports have described successful multidisciplinary treatment, there are currently no standard therapies for AFPGC. A 57-year-old man presented with upper abdominal pain. Read More

View Article
November 2018
1 Read

Radical nephrectomy and intracaval thrombectomy for advanced renal cancer with extensive inferior vena cava involvement utilising cardiopulmonary bypass and hypothermic circulatory arrest: Is it worthwhile?

Arab J Urol 2018 Dec 30;16(4):378-385. Epub 2018 Jul 30.

Department of Urology, University Hospital of Wales, Heath Park, Cardiff, UK.

Objective: To report our long-term outcomes of surgical treatment of renal tumours with inferior vena cava (IVC) tumour thrombus above the hepatic veins, utilising cardiopulmonary bypass (CBP) and hypothermic circulatory arrest (HCA), as surgical resection remains the only effective treatment for renal cancers with extensive IVC tumour thrombus.

Patients And Methods: We retrospectively reviewed 48 consecutive patients (median age 58 years) who underwent surgical treatment for non-metastatic renal cancer with IVC tumour thrombus extending above the hepatic veins. Perioperative, histological, disease-free (DFS) and overall survival (OS) data were recorded. Read More

View Article
December 2018

The roles of lncRNA in hepatic fibrosis.

Cell Biosci 2018 6;8:63. Epub 2018 Dec 6.

1Medical College, China Three Gorges University, 8 Daxue Road, Xiling District, Yichang, 443002 China.

Increasing evidence indicates that long non-coding RNAs (lncRNAs) regulate gene or protein expression; however, their function in the progression of hepatic fibrosis remains unclear. Hepatic fibrosis is a continuous wound-healing process caused by numerous chronic hepatic diseases, and the activation of hepatic stellate cells (HSCs) is generally considered to be a pivotal step in hepatic fibrosis. In the process of hepatic fibrosis, some lncRNAs regulates diverse cellular processes. Read More

View Article
December 2018