7,054 results match your criteria Hanging Injuries and Strangulation


Anatomy and ultrastructure of the tropical sponge Cladocroce caelum (Haplosclerida, Demospongiae).

J Morphol 2018 Dec;279(12):1872-1886

Instituto de Biologia, Universidade Federal da Bahia. Rua Barão de Jeremoabo, Salvador, Brazil.

The main characteristic of sponges (Porifera) is the presence of the aquiferous system-a system formed by canals and choanocyte chambers, in which the sponges carry out most of their physiological functions. Despite of the importance for the biology of the group, the knowledge about this structure is still incipient, even when morphological investigations are taken in account. Here, we investigated the anatomy and ultrastructure of the tropical demosponge Cladocroce caelum (Haplosclerida, Demospongiae) using light and electron microscopy. Read More

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December 2018

A reproducible scaffold-free 3D organoid model to study neoplastic progression in breast cancer.

J Cell Commun Signal 2018 Dec 4. Epub 2018 Dec 4.

Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA.

While 3D cellular models are useful to study biological processes, gel-embedded organoids have large variability. This paper describes high-yield production of large (~1 mm diameter), scaffold-free, highly-spherical organoids in a one drop-one organoid format using MCF10A cells, a non-tumorigenic breast cell line. These organoids display a hollow lumen and secondary acini, and express mammary gland-specific and progenitor markers, resembling normal human breast acini. Read More

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December 2018

Correlation of height and maximum overhead reach of a person in the forensic scenario: A study among the North Indian population.

Med Sci Law 2018 Dec 4:25802418816283. Epub 2018 Dec 4.

Department of Forensic Medicine, ESIC Medical College, India.

This study formulated a regression equation to estimate the maximum overhead reach of a person using his/her height. Height and maximum overhead reach of participants aged 21-30 years were measured. A regression equation was formulated to find the relation between height and maximum overhead reach. Read More

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December 2018

Peritoneal fluid stimulates neoplastic transformation of normal HEK 293 cells by high expression of pluripotent genes.

Pol J Pathol 2018 ;69(3):399-310

Gynecological cancers constitute a serious problem in the world. Their advanced stages are often characterized by the accumulation of ascites, which leads to spreading of cancer cells outside their primary focus. Despite progress in the treatment, prognoses are still not satisfactory. Read More

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January 2018

Hydroxysafflor yellow A promotes α-synuclein clearance via regulating autophagy in rotenone-induced Parkinson's disease mice.

Folia Neuropathol 2018 ;56(2):133-140

Autophagy is an intracellular degradation process that is involved in α-synuclein (α-syn) homeostasis and Parkinson's disease (PD). The purpose of this study was to investigate whether hydroxysafflor yellow A (HSYA) could promote α-syn clearance via regulating autophagy in PD mice. Male C57BL/6 mice were intraperitoneally treated with HSYA. Read More

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January 2018

Severe whipworm (Trichuris spp.) infection in the hamadryas baboon (Papio hamadryas).

J Vet Med Sci 2018 Nov 21. Epub 2018 Nov 21.

College of Veterinary Medicine, Kyungpook National University.

A 3-year-old male hamadryas baboon (Papio hamadryas) at the Seoul Zoo, Korea, died without any previous symptoms. Necropsy revealed severe whipworm infection in the large intestine. The animal weighed 2. Read More

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November 2018
5 Reads

Arbutin attenuates behavioral impairment and oxidative stress in an animal model of Parkinson's disease.

Avicenna J Phytomed 2018 Nov-Dec;8(6):533-542

Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

Objective: Arbutin has been shown to have antioxidant and free-radical scavenging properties. The aim of this study was to investigate the effects of arbutin administration on behavioral impairment, and oxidative and nitrosative stress in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced animal model of Parkinson's disease (PD).

Materials And Methods: PD model was developed by 4 intraperitoneal (i. Read More

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November 2018
2 Reads

Tension Pneumoperitoneum after Hanging.

Am Surg 2018 Mar;84(3):e108-e109

Division of Trauma and Critical Care, Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, California, USA.

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Development of Alumina⁻Mesoporous Organosilica Hybrid Materials for Carbon Dioxide Adsorption at 25 °C.

Materials (Basel) 2018 Nov 16;11(11). Epub 2018 Nov 16.

Department of Chemistry and Biochemistry, Kent State University, Kent, OH 44242, USA.

Two series of alumina (Al₂O₃)⁻mesoporous organosilica (Al⁻MO) hybrid materials were synthesized using the co-condensation method in the presence of Pluronic 123 triblock copolymer. The first series of Al⁻MO samples was prepared using aluminum nitrate nanahydrate (Al⁻NN) and aluminum isopropoxide (Al⁻IP) as alumina precursors, and organosilanes with three different bridging groups, namely tris[3-(trimethoxysilyl)propyl]isocyanurate, 1,4-bis(triethoxysilyl)benzene, and bis(triethoxysilyl)ethane. The second series was obtained using the aforementioned precursors in the presence of an amine-containing 3-aminopropyltriethoxysilane to introduce, also, hanging groups. Read More

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November 2018
2 Reads

Pneumocephalus after lumbar epidural.

Med J Armed Forces India 2018 Oct 28;74(4):397-399. Epub 2017 Nov 28.

Resident (Anaesthesia), Command Hospital (Western Command), Chandimandir, India.

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October 2018

Crystal structures of a llama VHH antibody BCD090-M2 targeting human ErbB3 receptor.

F1000Res 2018 16;7:57. Epub 2018 Jan 16.

St. Petersburg National Research Academic University RAS, St. Petersburg, 194021, Russian Federation.

: The ability of ErbB3 receptor to functionally complement ErbB1-2 and induce tumor resistance to their inhibitors makes it a unique target in cancer therapy by monoclonal antibodies. Here we report the expression, purification and structural analysis of a new anti-ErbB3 single-chain antibody. : The VHH fragment of the antibody was expressed in cells as a SUMO fusion, cleaved by TEV protease and purified to homogeneity. Read More

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January 2018
7 Reads

Near-Hangings: Epidemiology, Injuries, and Investigations.

J Trauma Acute Care Surg 2018 Nov 15. Epub 2018 Nov 15.

Division of Trauma and Surgical Critical Care, LAC+USC Medical Center, University of Southern California, Los Angeles, CA.

Background: Near-hangings are an infrequent cause of trauma and the optimal work-up for these patients is unclear. The study objectives were to define the epidemiology, injury patterns, and use of investigations, including CTA neck, after near-hangings.

Methods: All patients presenting to LAC+USC Medical Center (2008-2015) after near-hanging (ICD-9 E913. Read More

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November 2018
2 Reads

Risk Factors in Pediatric Blunt Cervical Vascular Injury and Significance of Seatbelt Sign.

West J Emerg Med 2018 Nov 18;19(6):961-969. Epub 2018 Oct 18.

McGovern Medical School at The University of Texas Health Sciences Center, Department of Diagnostic and Interventional Radiology, Houston, Texas.

Introduction: Computed tomography angiography (CTA) is used to screen patients for cerebrovascular injury after blunt trauma, but risk factors are not clearly defined in children. This modality has inherent radiation exposure. We set out to better delineate the risk factors associated with blunt cervical vascular injury (BCVI) in children with attention to the predictive value of seatbelt sign of the neck. Read More

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November 2018
3 Reads

Death by hanging: a retrospective case-control investigation of the intervertebral disc vacuum phenomenon on PMCT.

Forensic Sci Med Pathol 2018 12 13;14(4):484-496. Epub 2018 Nov 13.

Department of Forensic Medicine and Imaging, Institute of Forensic Medicine, University of Zurich, Winterthurerstrasse 190/52, CH-8057, Zurich, Switzerland.

During hanging gravitational forces affect the spine. Intervertebral vacuum phenomenon (VP) implies that gas accumulations in the discs are caused by degeneration of the spine and trauma. It was hypothesized that VP detected on postmortem computed tomography (PMCT) has a higher incidence in hanging deaths, which can be correlated to age, degenerative spinal changes and type of hanging (complete-incomplete). Read More

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December 2018
7 Reads

Dual Sacrificial Molding: Fabricating 3D Microchannels with Overhang and Helical Features.

Micromachines (Basel) 2018 Oct 16;9(10). Epub 2018 Oct 16.

Engineering Product Development, Singapore University of Technology & Design, Singapore 487372, Singapore.

Fused deposition modeling (FDM) has become an indispensable tool for 3D printing of molds used for sacrificial molding to fabricate microfluidic devices. The freedom of design of a mold is, however, restricted to the capabilities of the 3D printer and associated materials. Although FDM has been used to create a sacrificial mold made with polyvinyl alcohol (PVA) to produce 3D microchannels, microchannels with free-hanging geometries are still difficult to achieve. Read More

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October 2018

Robotic Repeat Right Hepatectomy for Recurrent Colorectal Liver Metastasis.

Ann Surg Oncol 2018 Nov 9. Epub 2018 Nov 9.

Nove de Julho Hospital, São Paulo, Brazil.

Background: Repeat hepatectomy often is required for primary and metastatic tumors. The purpose of this video was to present a robotic repeat hepatectomy for recurrent colorectal metastasis after multiple previous open surgeries.

Patient: A 64-year-old man underwent open left colectomy complicated by anastomotic leak. Read More

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November 2018

Nano-Sized Lipidated Dendrimers as Potent and Broad-Spectrum Antibacterial Agents.

Macromol Rapid Commun 2018 Nov 8:e1800622. Epub 2018 Nov 8.

M. Gide, Dr. A. Nimmagadda, M. Su, M. Wang, Dr. P. Teng, C. Li, R. Gao, Dr. J. Cai, Department of Chemistry, University of South Florida, 4202 E. Fowler Ave, Tampa, FL, 33620, USA.

There is considerable interest in the development of antimicrobial polymers including dendrimers due to the ease of synthesis and low manufacturing cost compared to host defense peptides (HDPs). Herein, a new class of nanomaterials-lipidated amphiphilic dendrimers-is presented that mimic the antibacterial mechanism of HDPs by compromising bacterial cell membranes. Unlike conventional dendrimers that are prepared generation by generation symmetrically with molecular weight distribution, these lipidated dendrimers are prepared on the solid phase with a hanging lipid tail and precisely controlled structure. Read More

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November 2018
2 Reads
4.940 Impact Factor

Expansion and Characterization of Mesenchymal Stromal Cells from Peritoneal Dialysis Effluent in a Human Protein Medium.

Stem Cells Int 2018 3;2018:5868745. Epub 2018 Oct 3.

Department of Urologic Sciences, University of British Columbia, Vancouver, BC, Canada.

The therapeutic potential of mesenchymal stromal cells (MSCs) from various tissue origins have extensively been explored in both experimental and clinical studies, and peritoneal dialysis effluent-derived MSC (pMSC) may be an easily obtainable MSC source for clinical applications. In this study, we expanded and characterized the pMSCs after expansion in a human protein culture medium. The pMSCs were expanded in plastic dishes with the human protein medium. Read More

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October 2018
6 Reads

Note: Grid cork and septal cork syringe adapters: An effective tool for efficient greasing of 24-well crystallization trays.

Authors:
Naveen Vankadari

Rev Sci Instrum 2018 Oct;89(10):106103

Monash Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia.

Applying grease to seal the well surface of the crystallization plate through traditional approaches in the hanging drop vapor diffusion method is a laborious process and known to cause air bubble formation. Here we report a simple design of adapters to the regular syringes for applying grease to the 24-well crystallization tray. This newly developed tool overcomes the difficulties faced with the traditional greasing methods, such as uneven distribution and excess of grease on the wells. Read More

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October 2018

How best to expose the entire surgical anal canal in the operative field during transanal pull-through for Hirschsprung's disease: a crucial step that determines success.

Pediatr Surg Int 2018 Nov 1. Epub 2018 Nov 1.

Department of Pediatric General and Urogenital Surgery, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.

Purpose: During transanal pull-through (TAPT) for Hirschsprung's disease (HD), exposing the entire surgical anal canal (SAC) including the squamo-columnar junction, or anorectal line (ARL) is a crucial step for minimizing problematic postoperative bowel function. We present a hint for exposing the entire SAC.

Method: Histologically, the ARL represents the junction of proximal unilayer columnar colorectal mucosa with distal stratified squamous epithelium and is the proximal limit of the SAC. Read More

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November 2018
3 Reads

Testing the Kundera Hypothesis: Does Every Woman (But Not Every Man) Prefer Her Child to Her Mate?

Evol Psychol 2018 Oct-Dec;16(4):1474704918808864

1 Departamento de Psicología, Universitat Jaume I, Castellón de la Plana, Spain.

The context of a famous novel by Milan Kundera ( Immortality) suggests that when faced with a life-or-death situation, every woman would prefer to save her child than her husband, left hanging whether every man would do the same. We labeled this as the Kundera hypothesis, and the purpose of this study was to test it empirically as we believe it raises a thought-provoking question in evolutionary terms. Specifically, 197 college students (92 women) were presented a questionnaire where they had to make different decisions about four dilemmas about who to save (their mate or their offspring) in two hypothetical life-or-death situations: a home fire and a car crash. Read More

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November 2018

Always stay cheerful - health information in the 1920s.

Tidsskr Nor Laegeforen 2018 10 30;138(17). Epub 2018 Oct 30.

Background: The interwar period was a time of comprehensive preventive health programmes in Norway. Physical exercise, nutritious diets, strict sleep regimens and better hygiene were at the centre of these efforts. A massive mobilisation of volunteers and professionals took place. Read More

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October 2018
3 Reads

Predictors of Futility in Near-Hanging and Therapeutic Hypothermia.

Ther Hypothermia Temp Manag 2018 Oct 30. Epub 2018 Oct 30.

WPP General Surgery/Trauma, WakeMed Health and Hospitals , Raleigh, North Carolina.

Favorable neurologic outcomes have been reported in near-hanging (NH) victims treated with therapeutic hypothermia (TH), but variable methods and small samples sizes limit interpretability. This study examines the relationship between clinical predictors, TH, and outcomes in NH patients. A risk profile was created by examining relationships between variables. Read More

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October 2018
1 Read

Single-drop microextraction combined with gas chromatography-electron capture detection for the determination of acrylamide in food samples.

Food Chem 2019 Feb 25;274:55-60. Epub 2018 Aug 25.

Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.

A single-drop microextraction method followed by gas chromatography-electron capture detection was developed to determine acrylamide in food samples. Acrylamide was extracted by water and derivatized by hydrobromic acid in the presence of ammonium peroxydisulfate. The derivatization was carried out at 45 °C in 15 min using 46 µL of hydrobromic acid and 98 mg of ammonium peroxydisulfate. Read More

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February 2019

Citrus Tangeretin Improves Skeletal Muscle Mitochondrial Biogenesis via Activating the AMPK-PGC1-α Pathway In Vitro and In Vivo: A Possible Mechanism for Its Beneficial Effect on Physical Performance.

J Agric Food Chem 2018 Nov 6;66(45):11917-11925. Epub 2018 Nov 6.

Key Lab of Physical Fitness Evaluation and Motor Functional Monitoring , General Administration of Sport of China-Southwest University , Chongqing 400715 , China.

Mitochondrial biogenesis is a key factor, which influences the function of skeletal muscle. Increasingly, flavonoids are reported to have the potential ability of regulating mitochondrial biogenesis. In this study, we investigated the effects of tangeretin, a polymethoxylated flavonoid isolated from mandarin fruits, on mitochondrial biogenesis and its underlying mechanisms. Read More

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November 2018
10 Reads

Chemogenetic activation of glutamatergic neurons in the motor cortex promotes functional recovery after ischemic stroke in rats.

Behav Brain Res 2018 Oct 23;359:81-88. Epub 2018 Oct 23.

Department of Rehabilitation Medicine, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400038, China. Electronic address:

Ischemic stroke is a major cause of disability and mortality worldwide, while no unequivocally efficacious drug is currently available to treat post-stroke functional impairments. Animal and clinical investigations suggest that the motor cortex stimulation constitutes a particularly promising approach for promoting function recovery after stroke. However, the cell types and mechanisms involved in stimulation-induced recovery are not well understood. Read More

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October 2018
2 Reads

Laparoscopic Right Hepatectomy Via an Anterior Approach for Hepatocellular Carcinoma.

JSLS 2018 Jan-Mar;22(1)

Department of Liver Surgery, The First People's Hospital of Foshan, Guang Dong, The People's Republic of China.

Background And Objectives: In the past, right hepatectomy via the anterior approach has been regarded as one of the many standard approaches for hepatectomy. However, total laparoscopic right hepatectomy from the anterior approach has been regarded as technically challenging. We report our experience in using the anterior approach in total laparoscopic right hepatectomy for hepatocellular carcinoma (HCC). Read More

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October 2018
1 Read
0.790 Impact Factor

Adsorption of colour from dye wastewater effluent of a down-flow hanging sponge reactor on purified coconut fibre.

Environ Technol 2018 Nov 15:1-10. Epub 2018 Nov 15.

a Department of Civil and Environmental Engineering , Nagaoka University of Technology , Nagaoka , Japan.

This study was carried out to evaluate the potential application of agricultural waste coconut (Cocos nucifera) fibre in its purified form for decolorization of dye-containing effluent from a down-flow hanging sponge (DHS) reactor. Batch adsorption experiments were conducted by varying adsorbent dose, solution pH, contact time and temperature. The results showed that the adsorption percentage of colour increased with increasing in adsorbent dosage from 0. Read More

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November 2018
6 Reads

Cervical seatbelt sign is not associated with blunt cerebrovascular injury in children: A review of the national trauma databank.

Am J Surg 2018 Oct 15. Epub 2018 Oct 15.

Division of Pediatric Surgery, Duke University Medical Center, Durham, NC, USA. Electronic address:

Background: Blunt cerebrovascular injury (BCVI) is a rare consequence of blunt trauma. There appears to be benefit to an aggressive approach to screening for BCVI due to catastrophic sequelae of unrecognized injury. However, screening for BCVI carries extensive cost and oncologic risk to young patients. Read More

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October 2018

Characterization of downflow hanging sponge reactors with regard to structure, process function, and microbial community compositions.

Appl Microbiol Biotechnol 2018 Dec 20;102(24):10345-10352. Epub 2018 Oct 20.

Department of Civil and Environmental Engineering, Nagaoka University of Technology, 1603-1 Kamitomioka-machi, Nagaoka, Niigata, 940-2188, Japan.

The activated sludge (AS) process has been the most widely used process for wastewater treatment despite it has several limitations for its further application and adoption worldwide, owing to unsustainable properties such as high-energy consumption and the production of large amount of excess sludge. To overcome the drawbacks of the AS process, the downflow hanging sponge (DHS) has been developed as an energy-saving and easy-to-maintain alternative. To date, six types of different sponge configurations have been developed and their performances have been evaluated in practical- to full-scale DHS reactors. Read More

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December 2018
1 Read

Pre-validation study of alternative developmental toxicity test using mouse embryonic stem cell-derived embryoid bodies.

Food Chem Toxicol 2018 Oct 16;123:50-56. Epub 2018 Oct 16.

Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, 28644, Republic of Korea. Electronic address:

The embryoid body test (EBT) is a developmental toxicity test method that assesses the half inhibitory concentrations of substances in the area of embryoid bodies (EBs), and in the viability of mouse embryonic stem cells (ESCs) and fibroblasts (3T3 cells) following chemical exposure for three and four days, respectively. In the previous study, the EBT showed more advanced than the embryonic stem cell test (EST) from the European Centre for the Validation of Alternative Methods (ECVAM) applying cardiac differentiation of mouse ESCs, because the EBT greatly reduced the exposure time, labor, and amount of materials required, and misclassification of embryotoxic potential. This pre-validation study evaluated the predictive accuracy of the EBT using 26 coded test substances by two steps: intra-laboratory and inter-laboratory reproducibility tests. Read More

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October 2018
4 Reads

Comparison of different in vitro differentiation conditions for murine female germline stem cells.

Cell Prolif 2018 Oct 17:e12530. Epub 2018 Oct 17.

Key Laboratory for the Genetics of Developmental & Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, Shanghai, China.

Objectives: In vitro differentiation of oocytes from female germline stem cells (FGSCs) has exciting potential applications for reproductive medicine. Some researchers have attempted to reveal the in vitro differentiation capacity of FGSCs. However, no systematic comparative study of in vitro differentiation conditions has been performed for murine FGSCs (mFGSCs). Read More

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October 2018
7 Reads

Highly efficient fog harvesting on superhydrophobic microfibers through droplet oscillation and sweeping.

Soft Matter 2018 Nov 15;14(41):8276-8283. Epub 2018 Oct 15.

Applied Mechanics of Materials Laboratory, Department of Mechanical Engineering, Temple University, 1947 North 12th Street, Philadelphia, PA 19122, USA.

Water droplet transport on fibers is of great importance for achieving high water collection efficiency from fog. Here, we exploit a new droplet sliding mechanism to accelerate the droplet coalescence and collection for highly efficient fog harvesting by coating hydrophilic microfibers with superhydrophobic layers of assembled carbon nanoparticles. We find that during the initial water collection, unlike the pinned droplets having axisymmetric barrel shapes wrapped around uncoated microfibers, the hanging droplets on coated microfibers with non-wrapping clamshell shapes are highly mobile due to their lower contact hysteresis adhesion; these are observed to oscillate, coalesce, and sweep the growing droplets along the horizontally placed microfibers. Read More

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November 2018
1 Read

Parameters of Surface Electromyogram Suggest That Dry Immersion Relieves Motor Symptoms in Patients With Parkinsonism.

Front Neurosci 2018 26;12:667. Epub 2018 Sep 26.

Biosignal Analysis and Medical Imaging Group, Department of Applied Physics, Faculty of Science and Forestry, University of Eastern Finland, Kuopio, Finland.

Dry immersion (DI) is acknowledged as a reliable space flight analog condition. At DI, subject is immersed in water being wrapped in a waterproof film to imitate microgravity (μG). Microgravity is known to decrease muscle tone due to deprivation of the sensory stimuli that activate the reflexes that keep up the muscle tone. Read More

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September 2018

Diagnosis and treatment of anterior canal benign paroxysmal positional vertigo.

Int J Audiol 2018 Sep 15;57(9):673-680. Epub 2018 Oct 15.

a Peking University Aerospace School of Clinical Medicine , Beijing , PR China.

Objective: To investigate the diagnosis and treatment of anterior canal benign paroxysmal positional vertigo (AC-BPPV).

Design: Retrospective analysis of clinical data regarding the diagnosis and treatment of patients with AC-BPPV.

Study Sample: Six patients with AC-BPPV. Read More

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September 2018
1 Read

Resveratrol restores sensitivity of glioma cells to temozolamide through inhibiting the activation of Wnt signaling pathway.

J Cell Physiol 2018 Oct 14. Epub 2018 Oct 14.

Department of Neurosurgery, Henan Provincial People's Hospital (People's Hospital of Zhengzhou University), Zhengzhou, China.

Malignant gliomas are aggressive primary neoplasms that originate in the glial cells of the brain or the spine with notable resistance to standard treatment options. We carried out the study with the aim to shed light on the sensitization of resveratrol to temozolomide (TMZ) against glioma through the Wnt signaling pathway. Initially, glioma cell lines with strong resistance to TMZ were selected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Read More

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October 2018
2 Reads

Accuracy, Repeatability, and Limitations for Determination of Chemical Activities from Vapor Pressure Osmometry.

Anal Chem 2018 Nov 19;90(21):12761-12767. Epub 2018 Oct 19.

Vapor pressure osmometry presents a convenient method to measure chemical activity. The work presented here was carried out to provide confidence in using this technique for a VPO model that does not utilize the "hanging-drop" method. While validation studies are available for certain models of vapor pressure osmometers, none were located for the UIC Jupiter 833 osmometer. Read More

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November 2018
6 Reads

Porphyromonas gingivalis bypasses epithelial barrier and modulates fibroblastic inflammatory response in an in vitro 3D spheroid model.

Sci Rep 2018 Oct 8;8(1):14914. Epub 2018 Oct 8.

INSERM (French National Institute of Health and Medical Research), UMR 1260, Regenerative Nanomedicine (RNM), Fédération de Médecine Translationnelle de Strasbourg (FMTS), 11 rue Humann, Strasbourg, 67000, France.

Porphyromonas gingivalis-induced inflammatory effects are mostly investigated in monolayer cultured cells. The aim of this study was to develop a 3D spheroid model of gingiva to take into account epithelio-fibroblastic interactions. Human gingival epithelial cells (ECs) and human oral fibroblasts (FBs) were cultured by hanging drop method to generate 3D microtissue (MT) whose structure was analyzed on histological sections and the cell-to-cell interactions were observed by scanning and transmission electron microscopy (SEM and TEM). Read More

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October 2018
2 Reads

Generation of Human Mesenchymal Stem Cell 3D Spheroids Using Low-binding Plates.

Bio Protoc 2018 Aug;8(16)

Division of Neuroscience and Experimental Neurology, Faculty of Biology, Medicine, and Health. University of Manchester, Manchester, UK.

The 3D culture of human mesenchymal stem cells (hMSCs) represents a more physiological environment than classical 2D culture and has been used to enhance the MSC secretome or extend cell survival after transplantation. Here we describe a simple and affordable method to generate 3D spheroids of hMSCs by seeding them at high density in a low-binding 96-well plate. Spheroids of hMSCs cultured in low-binding 96-well plates can be used to study the basic biology of the cells and to generate conditioned media or spheroids to be used in transplantation therapeutic approaches. Read More

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August 2018
1 Read

Deaths in custody in the Irish prison service: 5-year retrospective study of drug toxicology and unnatural deaths.

BJPsych Open 2018 Sep 21;4(5):401-403. Epub 2018 Sep 21.

Senior Registrar, National Forensic Mental Health Service, Central Mental Hospital, Dundrum and Department of Psychiatry, Trinity College Dublin, Ireland.

Mental health and substance misuse disorders are associated with unnatural deaths in prisoners. Deaths in Irish prisons between 2009 and 2014 were retrospectively analysed using coroner's findings, including post-mortem toxicology. There were 69 deaths in custody, 38 of which met inclusion criteria. Read More

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September 2018
1 Read

High prevalence of Gammapapillomaviruses (Gamma-PVs) in pre-malignant cutaneous lesions of immunocompetent individuals using a new broad-spectrum primer system, and identification of HPV210, a novel Gamma-PV type.

Virology 2018 Dec 4;525:182-191. Epub 2018 Oct 4.

Grupo Virología Humana, Instituto de Biología Molecular y Celular de Rosario (CONICET), Suipacha 590, 2000 Rosario, Argentina; Área Virología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, 2000 Rosario, Argentina. Electronic address:

Genus Gammapapillomavirus (Gamma-PV) is the most diverse and largest clade within the Papillomaviridae family. A novel set of degenerate primers targeting the E1 gene was designed and further used in combination with the well-known CUT PCR assay to assess HPV prevalence and genus distribution in a variety of cutaneous samples from 448 immunocompetent individuals. General HPV, Gamma-PV and mixed infections prevalence were significantly higher in actinic keratosis with respect to benign and malignant neoplasms, respectively (p = 0. Read More

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December 2018
7 Reads