J Glob Antimicrob Resist 2021 Mar 24;25:193-198. Epub 2021 Mar 24.
Infectious Diseases Department, University Hospital of Dijon, 14 rue Paul Gaffarel, 21000, Dijon, France; Vivexia, Résidence Richelieu, 10 Boulevard Carnot, 21000, Dijon, France. Electronic address:
Objectives: As daptomycin adjunction is currently under clinical evaluation in the multicentre phase II AddaMAP study to improve the prognosis of pneumococcal meningitis, the present work aimed at evaluating the in vitro antimicrobial activity of daptomycin-based combinations against some of the most frequent species responsible for bacterial meningitis.
Methods: Clinically relevant strains of Streptococcus pneumoniae, Listeria monocytogenes, Haemophilus influenzae and Neisseria meningitidis were obtained from National Reference Centers. The antimicrobial activity of amoxicillin, cefotaxime and rifampicin, either alone or in association with daptomycin, was explored through the determination of minimum inhibitory concentration (MIC) and fractional inhibitory concentration index (FICI) as well as time-kill assay (TKA) using the broth microdilution method. Read More