17,049 results match your criteria Haemophilus Influenzae Infections

Comparison of the bactericidal effects of quinolones against low-susceptible .

J Med Microbiol 2021 Jun;70(6)

Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 1920392, Japan.

The increasing incidence of with decreased susceptibility to quinolones (quinolone low-susceptible ) in Japan has raised concerns about therapeutic failure. Thus, assessment of effective antimicrobial agents is necessary to establish an effective therapeutic strategy against resulting infections. In this study, bactericidal effects of quinolones on low-susceptible strains were evaluated using time-kill curve analysis. Read More

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Phase Variation in HMW1A Controls a Phenotypic Switch in Haemophilus influenzae Associated with Pathoadaptation during Persistent Infection.

mBio 2021 Jun 22:e0078921. Epub 2021 Jun 22.

Instituto de Agrobiotecnología, Consejo Superior de Investigaciones Científicas (IdAB-CSIC)-Gobierno de Navarra, Mutilva, Spain.

Genetic variants arising from within-patient evolution shed light on bacterial adaptation during chronic infection. Contingency loci generate high levels of genetic variation in bacterial genomes, enabling adaptation to the stringent selective pressures exerted by the host. A significant gap in our understanding of phase-variable contingency loci is the extent of their contribution to natural infections. Read More

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Narrowing antibiotic spectrum of activity for trauma-associated pneumonia through the use of a disease-specific antibiogram.

Trauma Surg Acute Care Open 2021 3;6(1):e000602. Epub 2021 Jun 3.

Department of Pharmacy Services, St Joseph's Hospital and Medical Center, Phoenix, Arizona, USA.

Background: Organism susceptibilities for trauma-associated pneumonia (TAP) differ from those in other patient populations, including the critically ill. The purpose of this study was to identify common organisms and their susceptibilities in the respiratory isolates of trauma patients diagnosed with pneumonia within the first 7 days of hospital admission, and to create a TAP-specific disease-state antibiogram to guide empiric antibiotic therapy in this patient population.

Methods: This study was a retrospective review of adult trauma patients with pneumonia admitted between September 1, 2015 and August 31, 2018. Read More

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Learning from -omics strategies applied to uncover host-pathogen interactions: Current status and perspectives.

Comput Struct Biotechnol J 2021 15;19:3042-3050. Epub 2021 May 15.

Instituto de Agrobiotecnología, Consejo Superior de Investigaciones Científicas (IdAB-CSIC)-Gobierno de Navarra, Mutilva, Spain.

has contributed to key bacterial genome sequencing hallmarks, as being not only the first bacterium to be genome-sequenced, but also starring the first genome-wide analysis of chromosomes directly transformed with DNA from a divergent genotype, and pioneering Tn-seq methodologies. Over the years, the phenomenal and constantly evolving development of -omic technologies applied to a whole range of biological questions of clinical relevance in the -host interplay, has greatly moved forward our understanding of this human-adapted pathogen, responsible for multiple acute and chronic infections of the respiratory tract. In this way, essential genes, virulence factors, pathoadaptive traits, and multi-layer gene expression regulatory networks with both genomic and epigenomic complexity levels are being elucidated. Read More

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Arerugi 2021 ;70(4):310-314

Department of Pediatrics, Saitama Medical University Hospital.

A 12-year-old boy visited our hospital with complaints of chronic cough and dyspnea. Chest X-ray and CT revealed diffuse granular shadow in the bilateral lung fields and "Tree-in-bud appearance" in the peripheral airways, respectively. Sinusitis was present, and restrictive disorder was predominantly found in pulmonary function. Read More

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The interface between COVID-19 and bacterial healthcare-associated infections.

Ronan F O'Toole

Clin Microbiol Infect 2021 Jun 7. Epub 2021 Jun 7.

Department of Pharmacy and Biomedical Sciences, School of Molecular Sciences, College of Science, Health and Engineering, La Trobe University, Victoria, Australia. Electronic address:

Background: A wide range of bacterial infections occur in COVID-19 patients, particularly, in those with severe coronaviral disease. Some of these are community acquired co-infections.

Objective: To review recent data which indicate the occurrence of hospital-onset bacterial infections, including with antibiotic-resistant isolates, in COVID-19 patients. Read More

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Co-infections, secondary infections, and antimicrobial use in patients hospitalised with COVID-19 during the first pandemic wave from the ISARIC WHO CCP-UK study: a multicentre, prospective cohort study.

Lancet Microbe 2021 Jun 2. Epub 2021 Jun 2.

Medical Research Council-University of Glasgow Centre for Virus Research, University of Glasgow, Glasgow, UK.

Background: Microbiological characterisation of co-infections and secondary infections in patients with COVID-19 is lacking, and antimicrobial use is high. We aimed to describe microbiologically confirmed co-infections and secondary infections, and antimicrobial use, in patients admitted to hospital with COVID-19.

Methods: The International Severe Acute Respiratory and Emerging Infections Consortium (ISARIC) WHO Clinical Characterisation Protocol UK (CCP-UK) study is an ongoing, prospective cohort study recruiting inpatients from 260 hospitals in England, Scotland, and Wales, conducted by the ISARIC Coronavirus Clinical Characterisation Consortium. Read More

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Pyogenic lung abscess in an infectious disease unit: a 20-year retrospective study.

Ther Adv Respir Dis 2021 Jan-Dec;15:17534666211003012

Department of Infectious Diseases, AP-HP Hôpital Tenon, Sorbonne Université, Paris, France.

Background: Pyogenic lung abscesses are rare and poorly described infections. This study aimed to describe their prognostic factors.

Methods: We retrospectively included all patients hospitalized between 1 January 1998 and 1 June 2018, with an International Classification of Diseases, version 10 (IDC-10) diagnosis of pyogenic lung abscess, from the Diamm based medical records (Micro6, Nancy, France). Read More

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Invasive Haemophilus influenzae Infections after 3 Decades of Hib Protein Conjugate Vaccine Use.

Clin Microbiol Rev 2021 Jun 2;34(3):e0002821. Epub 2021 Jun 2.

Medical Sciences Division, Northern Ontario School of Medicine, Lakehead University, Thunder Bay, Ontario, Canada.

Haemophilus influenzae serotype b (Hib) was previously the most common cause of bacterial meningitis and an important etiologic agent of pneumonia in children aged <5 years. Its major virulence factor is the polyribosyl ribitol phosphate (PRP) polysaccharide capsule. In the 1980s, PRP-protein conjugate Hib vaccines were developed and are now included in almost all national immunization programs, achieving a sustained decline in invasive Hib infections. Read More

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In Vitro Time-Kill of Common Ocular Pathogens with Besifloxacin Alone and in Combination with Benzalkonium Chloride.

Pharmaceuticals (Basel) 2021 May 27;14(6). Epub 2021 May 27.

Bausch + Lomb, Rochester, NY 14609, USA.

Background: Besifloxacin ophthalmic suspension 0.6% (/%) contains benzalkonium chloride (BAK) as a preservative. We evaluated the in vitro time-kill activity of besifloxacin, alone and in combination with BAK, against common bacteria implicated in ophthalmic infections. Read More

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Pneumococcal and Influenza Vaccination Rates and Pneumococcal Invasive Disease Rates Set Geographical and Ethnic Population Susceptibility to Serious COVID-19 Cases and Deaths.

Vaccines (Basel) 2021 May 8;9(5). Epub 2021 May 8.

Department of Physiology, 567 Wilson Road, Room 1104 Biomedical and Physical Sciences Building, Michigan State University, East Lansing, MI 48824, USA.

This study examines the relationship of pneumococcal vaccination rates, influenza, measles-mumps-rubella (MMR) diphtheria-tetanus-pertussis vaccinations (DTP), polio, Haemophilus influenzae type B (Hib), and Bacillus Calmette-Guerin (tuberculosis) vaccination rates to COVID-19 case and death rates for 51 nations that have high rates of COVID-19 testing and for which nearly complete childhood, at-risk adult and elderly pneumococcal vaccination data were available. The study is unique in a large number of nations examined, the range of vaccine controls, in testing effects of combinations of vaccinations, and in examining the relationship of COVID-19 and vaccination rates to invasive pneumococcal disease (IPD). Analysis of Italian regions and the states of the United States were also performed. Read More

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SERS-based sensor for the detection of sexually transmitted pathogens in the male swab specimens: A new approach for clinical diagnosis.

Biosens Bioelectron 2021 Oct 21;189:113358. Epub 2021 May 21.

Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland. Electronic address:

The surface-enhanced Raman scattering (SERS) has been widely tested for its usefulness in microbiological studies, providing many information-rich spectra which are a kind of 'whole-organism fingerprint' and enabling identification of bacterial species. Here we show, previously not considered, the comprehensive SERS-chemometric analysis of five bacterial pathogens, namely Neisseria gonorrhoeae, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum, and Haemophilus ducreyi, all being responsible for sexually transmitted diseases (STDs). In the designed biosensor, the direct, intrinsic format of the spectroscopic analysis was adopted for the SERS-based screening of gonorrhea and chlamydiosis due to vibrational analysis of men's urethra swabs. Read More

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October 2021

Primary vaccination in adult patients after allogeneic hematopoietic stem cell transplantation - A single center retrospective efficacy analysis.

Vaccine 2021 May 25. Epub 2021 May 25.

Dept. of Internal Medicine III, University Hospital Regensburg, Franz-Joseph-Strauss-Allee 11, 93053 Regensburg, Germany. Electronic address:

Allogeneic hematopoietic stem cell transplantation (alloHSCT) results in a loss of humoral immunity and subsequent risk for severe infections. Thus, re-vaccination is required but may fail due to incomplete immune reconstitution. We retrospectively analyzed predictors of immune response to primary vaccination applied according to the EBMT (European Blood and Marrow Transplantation Group) recommendations. Read More

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Changes in the incidence of invasive disease due to Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis during the COVID-19 pandemic in 26 countries and territories in the Invasive Respiratory Infection Surveillance Initiative: a prospective analysis of surveillance data.

Lancet Digit Health 2021 06;3(6):e360-e370

Irish Meningitis and Sepsis Reference Laboratory, Children's Health Ireland at Temple Street, Dublin, Ireland; Royal College of Surgeons in Ireland, Beaumont Hospital, Dublin, Ireland.

Background: Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis, which are typically transmitted via respiratory droplets, are leading causes of invasive diseases, including bacteraemic pneumonia and meningitis, and of secondary infections subsequent to post-viral respiratory disease. The aim of this study was to investigate the incidence of invasive disease due to these pathogens during the early months of the COVID-19 pandemic.

Methods: In this prospective analysis of surveillance data, laboratories in 26 countries and territories across six continents submitted data on cases of invasive disease due to S pneumoniae, H influenzae, and N meningitidis from Jan 1, 2018, to May, 31, 2020, as part of the Invasive Respiratory Infection Surveillance (IRIS) Initiative. Read More

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COVID-19 containment measures and incidence of invasive bacterial disease.

Lancet Digit Health 2021 06;3(6):e331-e332

Epidemiology and Modelling of Antibiotic Evasion Unit, Institut Pasteur, Paris 75015, France; CESP, Université Paris-Saclay, UVSQ, Inserm, Montigny-Le-Bretonneux, France. Electronic address:

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Co-infection analysis of bacterial and viral respiratory pathogens from clinically healthy swine in Eastern China.

Vet Med Sci 2021 May 25. Epub 2021 May 25.

Institute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

Porcine respiratory disease complex (PRDC) is one of the most challenging health concerns for pig production worldwide. The aim of the present study was to determine the prevalence of pathogens associated with PRDC, including porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) and bacterial agents, such as Streptococcus suis, Haemophilus parasuis and Actinobacillus pleuropneumoniae, in clinically healthy pigs in Eastern China. Molecular detection revealed positive single-pathogen detection rates of 59. Read More

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PCV10 elicits Protein D IgG responses in Papua New Guinean children but has no impact on NTHi carriage in the first two years of life.

Vaccine 2021 Jun 21;39(26):3486-3492. Epub 2021 May 21.

Wesfarmers Centre of Vaccines and Infectious Diseases, Telethon Kids Institute, Western Australia, Australia; Centre for Child Health Research, University of Western Australia, Perth, Australia. Electronic address:

Background: Nasopharyngeal colonisation with nontypeable Haemophilus influenzae (NTHi) is associated with development of infections including pneumonia and otitis media. The 10-valent pneumococcal conjugate vaccine (PCV10) uses NTHi Protein D (PD) as a carrier. Papua New Guinean children have exceptionally early and dense NTHi carriage, and high rates of NTHi-associated disease. Read More

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Development of a rapid multiplex PCR assay for the detection of common pathogens associated with community-acquired pneumonia.

Trans R Soc Trop Med Hyg 2021 May 20. Epub 2021 May 20.

Department of Laboratory Medicine, Changi General Hospital, 2 Simei Street 3, Singapore 529889, Singapore.

Background: Community-acquired pneumonia (CAP) is one of the most common infectious diseases and is a significant cause of mortality and morbidity globally. A microbial cause was not determined in a sizable percentage of patients with CAP; there are increasing data to suggest regional differences in bacterial aetiology. We devised a multiplex real-time PCR assay for detecting four microorganisms (Streptococcus pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae and Burkholderia pseudomallei) of relevance to CAP infections in Asia. Read More

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Etiological distribution of pertussis-like syndrome in 756 children in Chengdu.

Transl Pediatr 2021 Apr;10(4):984-989

Department of Pediatric Respiratory Medicine, Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

Background: To analyze and summarize the etiological distribution of pertussis-like syndrome in children and to provide a basis for clinical treatment.

Methods: A retrospective analysis was conducted of 756 children with pertussis-like syndrome hospitalized at the Department of Pediatric Respiratory Medicine of Chengdu Women's and Children's Central Hospital, 2015 to 2019. The etiological features were classified and reviewed. Read More

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Brazilian Protocol for Sexually Transmitted Infections, 2020: infections that cause genital ulcers.

Rev Soc Bras Med Trop 2021 17;54(suppl 1):e2020663. Epub 2021 May 17.

Ministério da Saúde, Secretaria de Vigilância em Saúde, Brasília, DF, Brasil.

Infections that cause genital ulcers are one of the themes comprising the Clinical Protocol and Therapeutic Guidelines for Comprehensive Care for People with Sexually Transmitted Infections, published by the Brazilian Ministry of Health in 2020. The Protocol and Guidelines have been developed based on scientific evidence and validated in discussions with specialists. This article addresses clinical genital ulcer syndrome caused by sexually transmitted infections and its most common etiological agents: Treponema pallidum (syphilis), herpes simplex virus-2 (genital herpes) and herpes simplex virus-1 (perioral herpes), Haemophilus ducreyi (chancroid), Chlamydia trachomatis serotypes L1, L2 and L3 (lymphogranuloma venereum), and Klebsiella granulomatis (donovanosis). Read More

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Nontypeable Otitis Media: Mastoiditis and Meningitis Complicated with Central Venous Thrombosis in an Immunocompetent Child.

Case Rep Infect Dis 2021 13;2021:8845200. Epub 2021 Mar 13.

Ataşehir Memorial Hospital, Clinic of Pediatrics, Istanbul, Turkey.

Implementation of the type B (Hib) conjugate vaccine brought about a reduction in the number of cases and morbidity from type B but an increase in nontypeable strain infections. Nontypeable (NTHi) commonly colonizes children's upper respiratory tract and causes otitis media, sinusitis, and bronchitis. Invasive NTHi diseases, such as meningitis and septicemia, have rarely been reported. Read More

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Biofilm characteristics and transcriptomic analysis of Haemophilus parasuis.

Vet Microbiol 2021 Jul 29;258:109073. Epub 2021 Apr 29.

Sichuan Provincial Key Laboratory of Animal Diseases and Human Health, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. Electronic address:

Haemophilus parasuis (H. parasuis) is a conditional pathogen with the ability to form biofilms which can lead to ineffective drug treatment and severe chronic infections resulting in significant economic losses to the pig industry. Currently, knowledge of biofilm formation by H. Read More

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The lower respiratory tract microbiome of critically ill patients with COVID-19.

Sci Rep 2021 05 12;11(1):10103. Epub 2021 May 12.

Operative Unit of Clinical Microbiology, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 9 via G. Massarenti, 40138, Bologna, Italy.

COVID-19 infection may predispose to secondary bacterial infection which is associated with poor clinical outcome especially among critically ill patients. We aimed to characterize the lower respiratory tract bacterial microbiome of COVID-19 critically ill patients in comparison to COVID-19-negative patients. We performed a 16S rRNA profiling on bronchoalveolar lavage (BAL) samples collected between April and May 2020 from 24 COVID-19 critically ill subjects and 24 patients with non-COVID-19 pneumonia. Read More

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Antimicrobial susceptibility testing results from 13 hospitals in Viet Nam: VINARES 2016-2017.

Antimicrob Resist Infect Control 2021 05 10;10(1):78. Epub 2021 May 10.

Oxford University Clinical Research Unit, National Hospital for Tropical Diseases, 78 Giai Phong, Dong Da, Hanoi, Viet Nam.

Objective: To analyse data from 2016-17 from a hospital-based antimicrobial resistance surveillance with national coverage in a network of hospitals Viet Nam.

Methods: We analysed data from 13 hospitals, 3 less than the dataset from the 2012-13 period. Identification and antimicrobial susceptibility testing data from the clinical microbiology laboratories from samples sent in for routine diagnostics were used. Read More

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A Series of Surgical Infections and Review of the Literature.

Surg Infect (Larchmt) 2021 May 7. Epub 2021 May 7.

University of Maryland Shore Regional at Easton, Easton, Maryland, USA.

(HPI) is a rare and underreported pathogen. causes respiratory, soft tissue, and central nervous system (CNS) infections, and endocarditis. Little data on HPI surgical infections are available, especially for intra-abdominal infections (IAI). Read More

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Pediatric flexible bronchoscopy: A single-center report.

Pediatr Pulmonol 2021 May 10. Epub 2021 May 10.

Department of Women's and Children's Health, University of Padova, Padua, Italy.

Introduction: Pediatric flexible laryngotracheal bronchoscopy (FB) is an integral part of diagnostics and treatment at tertiary pediatric respiratory centers.

Aim: FBs performed between 2013 and 2018 at our Pediatric Allergy and Respiratory Medicine Unit of the Department of Women's and Children's Health at Padua University were examined in terms of the indications, findings, and adverse events.

Materials And Methods: The electronic medical records of pediatric patients who underwent FB at least once between 1 January 2013 and 31 December 2018 were considered. Read More

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Microbial co-infections in COVID-19: Associated microbiota and underlying mechanisms of pathogenesis.

Microb Pathog 2021 Jul 4;156:104941. Epub 2021 May 4.

Department of Microbiology, University of Dhaka, Dhaka, 1000, Bangladesh; Jashore University of Science and Technology, Jashore, 7408, Bangladesh. Electronic address:

The novel coronavirus infectious disease-2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has traumatized the whole world with the ongoing devastating pandemic. A plethora of microbial domains including viruses (other than SARS-CoV-2), bacteria, archaea and fungi have evolved together, and interact in complex molecular pathogenesis along with SARS-CoV-2. However, the involvement of other microbial co-pathogens and underlying molecular mechanisms leading to extortionate ailment in critically ill COVID-19 patients has yet not been extensively reviewed. Read More

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Extensive microbiological respiratory tract specimen characterization in critically ill COVID-19 patients.

APMIS 2021 May 5. Epub 2021 May 5.

The Regional Department of Clinical Microbiology, Zealand University Hospital, Roskilde, Denmark.

Microbial co-infections may contribute to the pulmonary deterioration in COVID-19 patients needing intensive care treatment. The present study portrays the extent of co-infections in COVID-19 ICU patients. Conventional culture, molecular detections for atypical aetiologies, QiaStat-Dx® respiratory panel V2 detecting 21 respiratory pathogens and ribosomal DNA genes 16S/18S amplicon-based microbiome analyses were performed on respiratory samples from 34 COVID-19 patients admitted to the ICU. Read More

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Bacterial agents in vulvovaginitis and vaginal discharge: a 10-year retrospective study in the Netherlands.

Eur J Clin Microbiol Infect Dis 2021 May 3. Epub 2021 May 3.

Laboratory of Clinical Microbiology and Infectious Diseases, Isala Hospital, Dr. van Heesweg 2, 8025 AB, Zwolle, The Netherlands.

Vulvovaginitis is a common problem in the GP's practice. Causes are bacterial vaginosis (BV), Candida infection and sexually transmitted infections (STIs). Only if empirical treatment fails, a vaginal swab is sent in for culture and BV detection. Read More

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Construction and Application of MALDI-TOF Mass Spectrometry for the Detection of Haemophilus parasuis.

Biomed Res Int 2021 15;2021:5588855. Epub 2021 Apr 15.

Shanghai Animal Disease Control Center, 855 Hongjin Road, Shanghai 201103, China.

To construct a protein fingerprint database of (), thus improving its clinical diagnosis efficiency. A total of 15 standard strains were collected to establish a protein fingerprint database of using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), and the effects of different culture media and culture time on the quality and identification results of the protein fingerprint were investigated. The results showed that tryptone soy agar (TSA) and tryptone soy broth (TSB) media and different incubation times had no significant effect on the characteristic peaks of the protein profiles. Read More

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