154 results match your criteria Granulosa-Theca Cell Tumors

Inhibin-A and inhibin-B in cyclic and pregnant mares, and mares with granulosa-theca cell tumors: Physiological and diagnostic implications.

Theriogenology 2018 Mar 7;108:192-200. Epub 2017 Dec 7.

Ansh Labs LLC, Webster, TX, USA.

Studies in mares have examined serum inhibin concentrations using immuno-assays unable to distinguish dimeric inhibin-A from inhibin-B isoforms. Inhibin-A and inhibin-B immuno-assays were used to investigate concentrations in cyclic mares, young and old (6 vs 19 years old, respectively) mares following hemi-ovariectomy, mares during pregnancy and in mares with confirmed granulosa cell tumors (GCTs). Mares with inter-ovulatory intervals of 26 days had ovulatory peaks of inhibin-A averaging 80 pg/mL with a mid-cycle nadir of 5 pg/mL. Read More

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March 2018
4 Reads

FOXL2 Mutation Status in Granulosa Theca Cell Tumors of the Ovary.

Int J Gynecol Pathol 2017 Nov;36(6):568-574

Department of Pathology, University of California San Francisco, San Francisco (A.N., N.M.J., J.R., C.Z., K.G.) Department of Pathology, El Camino Hospital, Mountain View (A.R.S.), CA.

Ovarian sex-cord stromal tumors that have between 10% and 50% granulosa cells in a prominent fibrothecomatous background have been referred to as granulosa theca cell tumors or mixed granulosa theca cell tumors. The classification and prognosis of these tumors is not clear. Most adult granulosa cell tumors of the ovary harbor a mutation in the FOXL2 gene, whereas fibromas and thecomas lack this mutation. Read More

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November 2017
40 Reads

Evaluation of diagnostic utility, safety considerations, and effect on fertility of transvaginal ultrasound-guided ovarian biopsy in mares.

Theriogenology 2016 Apr 24;85(6):1030-6. Epub 2015 Nov 24.

Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.

Ultrasound-guided biopsies of corpora lutea have been previously used for research purposes in the mare and cow. However, the health effects and fertility after transvaginal luteal biopsies (TVLB) or transvaginal ovarian biopsies (TVOB) obtained for diagnostic purposes in cases of suspected ovarian tumors have not been previously evaluated in the horse. The aim of this study was to determine the effects on health and fertility of TVLB and TVOB in mares; 53 mares were included in the study (11 control non-biopsied mares, 37 TVLB mares biopsied on one or more of the following Days 8, 10, 12, 15, 21, and 5 TVOB mares with ovarian abnormalities), resulting in a total of 108 TVLB and TVOB cycles and 183 procedures. Read More

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April 2016
7 Reads

Expression of angiotensin II receptors in the caprine ovary and improvement of follicular viability in vitro.

Zygote 2016 Aug 14;24(4):568-77. Epub 2015 Oct 14.

Faculty of Veterinary Medicine,LAMOFOPA,PPGCV,State University of Ceara,Fortaleza-CE,Brazil.

This study aimed to evaluate mRNA levels of angiotensin II (ANG II) receptors (AGTR1 and AGTR2) in caprine follicles and to investigate the influence of ANG II on the viability and in vitro growth of preantral follicles. Real-time polymerase chain reaction (PCR) was used to quantify AGTR1 and AGTR2 mRNA levels in the different follicular stages. For culture, caprine ovaries were collected, cut into 13 fragments and then either directly fixed for histological and ultrastructural analysis (fresh control) or placed in culture for 1 or 7 days in α-minumum essential medium plus (α-MEM+) with 0, 1, 5, 10, 50 or 100 ng/ml ANG II. Read More

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August 2016
2 Reads

Management of Ovarian Cancer In Younger Women.

Rev Recent Clin Trials 2015 ;10(4):263-9

Department of Gynaec Oncology, Kidwai Memorial Institute of Oncology, Bangalore, Karnataka State, India.

Background: Ovarian Cancer is a broad spectrum of diseases comprising several subtypes. Three major categories in younger women are germ cell tumor, sex cord stromal tumor and epithelial ovarian neoplasia.

Objective: literature search was for an update on management of ovarian cancer in young women. Read More

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August 2016
19 Reads

Reproductive hormones affect follicular cells and ooplasm of Stage I and II oocytes in zebrafish.

Reprod Fertil Dev 2016 Oct;28(12):1945-1952

CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, CIMAR Associated Laboratory, UPorto - University of Porto, Laboratory of Cellular, Molecular and Analytical Studies, Rua dos Bragas 289, 4050-123 Porto, Portugal.

The basic pathway of oocyte development and its regulation is evolutionarily conserved among vertebrates; however, little is known about the role of hormones at the first stages (Stages I and II) of follicle development in fish. In the present study, zebrafish follicles at Stages I and II were exposed in vitro to the reproductive hormones 17β-oestradiol (E), 11-ketotestosterone (11KT), 17,20β-dihydroxy-4-pregnen-3-one (DHP) and to the secondary messenger dibutyryl cyclic adenosine monophosphate (db-cAMP) at a concentration of 1µM for a 48-h period. Morphological alterations of the ooplasm were assessed by transmission electron microscopy and of the granulosa cell layer by quantitative stereology. Read More

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October 2016

Spontaneous lactogenesis and galactopoeisis in a virgin dairy heifer with evidence of a granulosa theca cell tumour.

A J Gunn J Reader

N Z Vet J 2015 Nov 10;63(6):345-6. Epub 2015 Jul 10.

a School of Animal and Veterinary Science, and Graham Centre for Agricultural Innovation , Charles Sturt University , NSW 2678 , Australia.

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November 2015
5 Reads

Evidence of Spontaneous Recovery of Granulosa-Theca Cell Tumour in a Heifer: A Retrospective Report.

Reprod Domest Anim 2015 Aug 12;50(4):696-703. Epub 2015 Jun 12.

Laboratory of Theriogenology, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.

A 14-month-old Japanese Black heifer was evaluated on Day 0 (D 0) for enlargement of the right ovary (RO). Transrectal ultrasonography (TRUS) revealed that the RO was markedly enlarged and multicystic, while the left ovary (LO) was small and inactive. The presumptive diagnosis was granulosa-theca cell tumour (GTCT), which was confirmed by markedly elevated plasma anti-Müllerian hormone (AMH) of 4. Read More

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August 2015
8 Reads

Differential gene expression and immunolocalization of platelet-derived growth factors and their receptors in caprine ovaries.

Domest Anim Endocrinol 2015 Apr 20;51:46-55. Epub 2014 Oct 20.

Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil.

This study evaluated the messenger RNA (mRNA) expression and immunolocalization of all members of the platelet-derived growth factor (PDGF) family in caprine ovaries by quantitative PCR and immunohistochemistry, respectively. Detectable levels of PDGF-A mRNA were not observed in primordial follicles. Higher levels of PDGF-B mRNA were observed in primary follicles than in primordial follicles (P < 0. Read More

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April 2015
2 Reads

Molecular pathogenesis in granulosa cell tumor is not only due to somatic FOXL2 mutation.

J Ovarian Res 2014 Sep 6;7:88. Epub 2014 Sep 6.

Granulosa cell tumors are rare ovarian malignancies. Their characteristics include unpredictable late recurrent and malignant behavior. Recent molecular studies have characterized the FOXL2 402C > G mutation in adult-type granulosa cell tumor. Read More

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September 2014
12 Reads

Theriogenology question of the month. Granulosa-theca cell tumor.

J Am Vet Med Assoc 2014 Jul;245(2):179-82

Loomis Basin Equine Medical Center, 2973 Penryn Rd, Penryn, CA 95663.

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July 2014
6 Reads

Premature ovarian insufficiency - fertility challenge.

J H Check

Minerva Ginecol 2014 Apr;66(2):133-53

Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology Cooper Medical School of Rowan University, Camden, NJ, USA -

Premature ovarian insufficiency, defined as amenorrhea with estrogen deficiency in a woman younger than 40 associated with a serum follicle stimulating hormone (FSH) >35 mIU/mL, can be temporarily reversed with ovulation achieved resulting in live delivered pregnancies. Though this may occur spontaneously the frequency of ovulation can be considerably increased by various techniques of lowering the elevated serum FSH level and thus up-regulate down-regulated FSH receptors in the granulosa-theca cells. This can be accomplished by either suppressing FSH release from the pituitary by negative feedback through high dose estrogen or by suppressing FSH production by inhibiting the gonadotropin releasing hormone (GnRH) by either using GnRH agonists or antagonists. Read More

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April 2014
4 Reads

Presence of growth hormone receptor (GH-R) mRNA and protein in goat ovarian follicles and improvement of in vitro preantral follicle survival and development with GH.

Theriogenology 2014 Jul 2;82(1):27-35. Epub 2014 Mar 2.

Laboratory of Manipulation of Oocytes Enclosed in Preantral Follicles (LAMOFOPA), Veterinary Faculty, State University of Ceará, Fortaleza, Brazil.

This study aimed to demonstrate the expression of growth hormone receptor (GH-R) mRNA and protein in goat ovarian follicles in order to investigate the effects of GH on the survival and development of preantral follicles. The ovaries were processed for the isolation of follicles to study GH-R mRNA expression or to localization of GH-R by immunohistochemical analysis. Pieces of ovarian cortex were cultured for 7 days in minimum essential medium(+) (MEM(+)) in the presence or absence of GH at different concentrations (1, 10, 50, 100, and 200 ng/mL). Read More

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July 2014
1 Read

Plasma anti-Müllerian hormone as a biomarker for bovine granulosa-theca cell tumors: comparison with immunoreactive inhibin and ovarian steroid concentrations.

Theriogenology 2013 Nov 24;80(8):940-9. Epub 2013 Aug 24.

Laboratory of Theriogenology, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan; Department of Theriogenology, Faculty of Veterinary Medicine, University of Mansoura, Mansoura 35516, Egypt.

Granulosa-theca cell tumors (GTCTs) are the most frequently reported ovarian tumors in cattle. Clinically, GTCTs could be confused with other ovarian abnormalities; therefore, the only definitive diagnosis for such tumors is histopathology of a biopsy from the affected ovary. However, this is an invasive technique and unsuitable for farm conditions. Read More

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November 2013
6 Reads

Long-term outcome for two heifers with a granulosa-theca cell tumour.

Vet Rec 2013 Jun 1;172(22):581. Epub 2013 May 1.

School of Veterinary Science, University of Liverpool, Leahurst, Neston, Cheshire CH64 7TE, UK.

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June 2013
3 Reads

Letrozole increases ovarian growth and Cyp17a1 gene expression in the rat ovary.

Fertil Steril 2013 Mar 30;99(3):889-96. Epub 2012 Nov 30.

Department of Obstetrics and Gynecology, School of Medicine, University of California, Davis, California, IVI-Madrid, Madrid, Spain.

Objective: To evaluate the effects of letrozole on ovarian size and steroidogenesis in vivo, as well as on proliferation and steroidogenesis of theca-interstitial cells alone and in coculture with granulosa cells using an in vitro model.

Design: In vivo and in vitro studies.

Setting: Research laboratory. Read More

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March 2013
1 Read

Developmental programming: impact of prenatal testosterone excess on ovarian cell proliferation and apoptotic factors in sheep.

Biol Reprod 2012 Jul 26;87(1):22, 1-10. Epub 2012 Jul 26.

Department of Morphological Sciences, Faculty of Veterinary Sciences, National University of Litoral, Esperanza, Argentina.

Prenatal testosterone (T) excess leads to reproductive dysfunctions in sheep, which include increased ovarian follicular recruitment and persistence. To test the hypothesis that follicular disruptions in T sheep stem from changes in the developmental ontogeny of ovarian proliferation and apoptotic factors, pregnant Suffolk sheep were injected twice weekly with T propionate or dihydrotestosterone propionate (DHT; a nonaromatizable androgen) from Days 30 to 90 of gestation. Changes in developmental expression of proliferating cell nuclear antigen (PCNA), BCL2, BAX, activated CASP3, and FAS/FASLG were determined at Fetal Days 90 and 140, 22 wk, 10 mo, and 21 mo of age by immunocytochemisty. Read More

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July 2012
1 Read

BMPRIB and BMPRII mRNA expression levels in goat ovarian follicles and the in vitro effects of BMP-15 on preantral follicle development.

Cell Tissue Res 2012 Apr 2;348(1):225-38. Epub 2012 Mar 2.

Faculty of Veterinary, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil.

This study evaluated the levels of bone morphogenetic protein receptors BMPRIB and BMPRII mRNA in goat follicles and the effects of bone morphogenetic protein-15 (BMP-15) on the in vitro development of cultured preantral follicles. Real-time polymerase chain reaction (PCR) was used to analyze the levels of BMPRIB and BMPRII mRNA in caprine preantral follicles and in small and large antral follicles. Preantral follicles (≥150 μm) were also isolated from goat ovaries and cultured for 18 days in α-MEM(+) supplemented with or without BMP-15 (10, 50, or 100 ng/ml). Read More

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April 2012
5 Reads

DAN (NBL1) specifically antagonizes BMP2 and BMP4 and modulates the actions of GDF9, BMP2, and BMP4 in the rat ovary.

Biol Reprod 2012 May 31;86(5):158, 1-9. Epub 2012 May 31.

Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Taipei, Taiwan.

Although differential screening-selected gene aberrative in neuroblastoma (DAN, official symbol NBL1) is the founding member of the DAN subfamily of bone morphogenetic protein (BMP) antagonists, its antagonizing targets, gene regulation, and physiological functions remain unclear. Using diverse cell expression systems, we found that the generation of bioactive DAN is likely to be cell type specific. Unlike other phylogenetically close members, which are covalently linked homodimers, DAN forms a noncovalently linked homodimer during folding. Read More

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Granulosa theca cell tumour in a pregnant mare: concentrations of inhibin and testosterone in serum before and after surgery.

N Z Vet J 2012 Mar;60(2):160-3

Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Private Bag 11222, Palmerston North 4442, New Zealand.

Case History: A 14-year-old Thoroughbred mare was presented for evaluation of a rapidly enlarging ovary at 73 days gestation. The mare's owner reported behavioural changes in the mare since she returned from stud, notably aggression towards other horses.

Clinical Findings: Transrectal palpation and ultrasonography of the reproductive tract revealed a viable pregnancy, an enlarged, multiloculated right ovary, and a normal-sized left ovary with a corpus luteum present. Read More

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March 2012
5 Reads

Expression levels of mRNA-encoding PDGF receptors in goat ovaries and the influence of PDGF on the in vitro development of caprine pre-antral follicles.

Reprod Domest Anim 2012 Oct 3;47(5):695-703. Epub 2011 Dec 3.

Faculty of Veterinary, Laboratory of Manipulation of Oocytes and Preantral Follicles, PPGCV, State University of Ceará, Fortaleza, CE, Brazil.

The aims of this study were to investigate the expression levels of mRNA for platelet-derived growth factor (PDGF) receptors (PDGFR-α and -β) in caprine follicles at different developmental stages and to evaluate the influence of PDGF on the in vitro development of pre-antral follicles. For this, goat primordial, primary and secondary follicles, as well as small (1-3 mm) and large (3-6 mm) antral follicles, were obtained, and PDGFR-α and -β mRNA levels were quantified by real-time PCR. Furthermore, pre-antral follicles (≥ 200 μm) were isolated from goat ovaries and cultured for 18 days in α- minimum essential medium supplemented with PDGF at 50 or 100 ng/ml, containing or not FSH. Read More

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October 2012
1 Read

Anti-Müllerian hormone profiles as a novel biomarker to diagnose granulosa-theca cell tumors in cattle.

J Reprod Dev 2012 4;58(1):98-104. Epub 2011 Nov 4.

Laboratory of Theriogenology, University of Miyazaki, Miyazaki 889-2192, Japan.

This study was carried out to evaluate the blood profile and tissue expression of Anti-Müllerian hormone (AMH) as a biomarker for granulosa-theca cell tumors (GTCTs) in cattle. Five cases with unilateral ovarian GTCTs (GTCT group) were investigated in comparison to other groups of Japanese Black cows, which had either cystic ovarian disease (COD group, n=5), a functional corpus luteum on Days 9 to 11 of the estrous cycle (Day 0=estrus; CL group, n=13) or received superovulation treatment (SOT group, n=13). We used transrectal ultrasonography and measured plasma AMH, estradiol-17β (E(2)), progesterone (P(4)) and testosterone (T) levels. Read More

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July 2012
6 Reads

Levels of BMP-6 mRNA in goat ovarian follicles and in vitro effects of BMP-6 on secondary follicle development.

Zygote 2013 Aug 6;21(3):270-8. Epub 2011 Oct 6.

Biotechnology Nucleus of Sobral - NUBIS, Federal University of Ceara, Sobral, CE, Brazil.

Expression of BMP-6 mRNA was quantified by real-time polymerase chain reaction (PCR) and the BMP-6 protein was demonstrated by immunohistochemistry in the primordial, primary, secondary, small and large antral follicles of goat. Furthermore, the influence of BMP-6 on increase in diameter, antrum formation and expression of BMP-6 and FSH-R in in vitro cultured secondary follicles was studied. Therefore, goat primordial, primary and secondary follicles, as well as small and large antral follicles were obtained and the mRNA levels of BMP-6 were quantified by PCR in real time. Read More

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August 2013
1 Read

Expression patterns of poliovirus receptor, erythrocyte protein band 4.1-like 3, regulator of g-protein signaling 11, and oxytocin receptor in mouse ovarian cells during follicle growth and early luteinization in vitro and in vivo.

Biol Reprod 2012 Jan 16;86(1):1-11. Epub 2012 Jan 16.

Follicle Biology Laboratory, Vrije Universiteit Brussel, Brussels, Belgium.

Poliovirus receptor (Pvr), erythrocyte protein band 4.1-like 3 (Epb4.1l3), regulator of G-protein signaling 11 (Rgs11), and oxytocin receptor (Oxtr) expression were quantified in in vitro- and in vivo-grown mouse follicles. Read More

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January 2012
1 Read

Two atypical cases of granulosa cell tumours in Belgian Blue heifers.

Reprod Domest Anim 2011 Aug 18;46(4):746-9. Epub 2010 Nov 18.

Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.

This paper describes two atypical cases of granulosa cell tumours (GCTs) in Belgian Blue heifers. In the first case, an 18-month-old heifer died after ovariectomy, and a granulosa-theca cell tumour was diagnosed post-mortem. The second heifer (31 months of age) was near term and was successfully treated surgically for a malignant granulosa cell tumour. Read More

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August 2011
7 Reads

Neonatal exposure to bisphenol A or diethylstilbestrol alters the ovarian follicular dynamics in the lamb.

Reprod Toxicol 2011 Nov 21;32(3):304-12. Epub 2011 Jun 21.

School of Agricultural Sciences, Universidad Nacional de Lomas de Zamora, Buenos Aires, Argentina.

We hypothesized that neonatal xenoestrogen exposure affects the ovarian follicular dynamics in lambs. Female lambs were exposed from postnatal day (PND) 1-14 to low doses of diethylstilbestrol (DES) or bisphenol A (BPA). At PND 30, the follicular dynamics and ovarian biomarkers (ERα, ERβ, AR, Ki67, p27) were evaluated. Read More

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November 2011
1 Read

Gene expression and immunolocalization of fibroblast growth factor 2 in the ovary and its effect on the in vitro culture of caprine preantral ovarian follicles.

Reprod Domest Anim 2012 Feb 23;47(1):20-5. Epub 2011 Apr 23.

Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.

This study quantified Fibroblast growth factor 2 (FGF-2) mRNA and localized FGF-2 protein in different categories of follicles isolated from goat ovaries. In addition, we verified the effects of this factor on the in vitro culture of preantral follicles isolated from goats. For mRNA quantification, we performed real-time PCR using primordial, primary and secondary follicles, as well as cumulus-oocyte complexes (COCs) and mural granulosa and theca cells of small and large antral follicles. Read More

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February 2012
1 Read

Steady-state level of epidermal growth factor (EGF) mRNA and effect of EGF on in vitro culture of caprine preantral follicles.

Cell Tissue Res 2011 Jun 20;344(3):539-50. Epub 2011 Apr 20.

Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE, Brazil.

Our aim was to verify the steady-state level of epidermal growth factor (EGF) mRNA in goat follicles at various developmental stages and to investigate the influence of EGF on the survival, antrum formation and growth of secondary follicles cultured for 6 days. Primordial, primary and secondary goat follicles and small and large antral follicles were obtained to quantify EGF mRNA by real-time reverse transcription with the polymerase chain reaction. The influence of EGF and the presence or absence of follicle-stimulating hormone (FSH) on the development of secondary follicles and on mRNA expression for EGF and FSH receptor (FSH-R) was determined after 6 days of culture. Read More

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June 2011
7 Reads

Granulosa theca cell tumor with erythrocytosis in a llama.

Can Vet J 2010 Oct;51(10):1157-60

Department of Veterinary Clinical Sciences, The Ohio State University, Columbus, 43210, USA.

A 2.5-year-old, female llama with weight loss and lethargy had a packed cell volume (PCV) of 45% which increased to 57% over 3 wk. Transrectal ultrasonography revealed a mass of mixed echogenicity involving the right ovary, which was removed. Read More

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October 2010
3 Reads

Progestin signaling through mPRα in Atlantic croaker granulosa/theca cell cocultures and its involvement in progestin inhibition of apoptosis.

Endocrinology 2010 Dec 20;151(12):5916-26. Epub 2010 Oct 20.

University of Texas at Austin Marine Science Institute, Port Aransas, Texas 78373, USA.

Although there is substantial evidence that membrane progestin receptors (mPRs) perform a critical physiological role in meiotic maturation of fish oocytes, it is unknown whether they are also intermediaries in progestin signaling in the surrounding follicular cells. Here, we show that mPRα protein is located on the plasma membranes of both granulosa and theca cells (G/T cells) isolated from Atlantic croaker ovaries and is associated with the presence of a single high affinity, limited capacity, pertussis toxin-sensitive, specific progestin [17,20β,21-trihydroxy-4-pregnen-3-one (20β-S)] membrane binding site with the characteristics of mPRα. Treatment of G/T cells with 20β-S caused rapid G protein activation and a transient, pertussis toxin-sensitive, decrease in cAMP levels, whereas the selective nuclear progesterone receptor agonist, R5020, did not cause G protein activation, consistent with previous reports on mPRα signaling. Read More

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December 2010
1 Read

Cellular localization of androgen synthesis in equine granulosa-theca cell tumors: immunohistochemical expression of 17alpha-hydroxylase/17,20-lyase cytochrome P450.

Theriogenology 2010 Aug 22;74(3):393-401. Epub 2010 Apr 22.

Department of Population Health & Reproduction, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.

Elevated blood testosterone concentrations, often accompanied by male-typical behaviors, is a common signalment of mares with granulosa-theca cell tumors (GCTCs), but no definitive information exists regarding the cellular differentiation of tumors associated with androgen secretion. This study was conducted to localize and thereby define the cellular expression of 17alpha-hydroxylase/17,20-lyase cytochrome P450 (P450c17), the enzyme most directly responsible for androgen synthesis, in 30 GTCTs and control tissues (gonads and adrenal glands) using immuno-histochemistry (IHC). Immuno-reactivity for P450c17 was evident in approximately half of 30 specimens examined, was most consistent in the interstitial cells surrounding existing or developing cysts, and was less intense in cells within cysts in the smaller proportion of specimens where this was observed. Read More

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August 2010
5 Reads

Evaluation of a motorized morcellator for laparoscopic removal of granulosa-theca cell tumors in standing mares.

Vet Surg 2010 Jul 25;39(5):649-53. Epub 2010 Mar 25.

Equine Department, Section Surgery, Vetsuisse-Faculty, University of Zurich, CH-8057 Zurich, Switzerland.

Objective: To describe a motorized morcellator technique for laparoscopic removal of granulosa-theca cell tumors (GCT) in standing mares and to evaluate long-term outcome.

Study Design: Case series.

Animals: Mares (n=7) aged 4-15 years, with unilateral GCT. Read More

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July 2010
5 Reads

The concept and treatment methodology for inducing ovulation in women in apparent premature menopause.

J H Check

Clin Exp Obstet Gynecol 2009 ;36(2):70-3

Purpose: To provide the concept and details of the methodology of inducing ovulation in women in apparent menopause.

Methods: A recent case is discussed and other previous publications described illustrating how to induce ovulation and achieve pregnancies despite what appears to be menopause. The various methods of lowering serum follicle stimulating hormone (FSH) and restoring down-regulated FSH receptors in granulosa theca cells of the follicle are described. Read More

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October 2009
2 Reads

Oocyte-granulosa-theca cell interactions during preantral follicular development.

J Ovarian Res 2009 Jul 9;2(1). Epub 2009 Jul 9.

Department of Obstetrics & Gynecology, University of Fukui, Matsuoka, Fukui, 910-1193, Japan.

The preantral-early antral follicle transition is the penultimate stage of follicular development in terms of gonadotropin dependence and follicle destiny (growth versus atresia). Follicular growth during this period is tightly regulated by oocyte-granulosa-theca cell interactions. Formation of the theca cell layer is a key event that occurs during this transitional stage. Read More

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Compilation of classical and contemporary terminology used to describe morphological aspects of ovarian dynamics in cattle.

Theriogenology 2009 Jun 1;71(9):1343-57. Epub 2009 Apr 1.

Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA.

Veterinarians and scientists involved in applied and basic research in cattle require a lexicon of terms that is used uniformly so that diagnoses and inference of results between and among studies can be correctly interpreted and substantiated or negated and therapy and hypotheses can be formulated without unnecessary confusion and redundancy in treatments and experiments. This review provides a compilation of many of the classical and contemporary terms used in association with ovarian dynamics primarily during the estrous cycle in cattle, which can also apply to other reproductive states. While many classical terms used to describe healthy and diseased conditions associated with follicles and corpora lutea are still applicable today, there are some that have become antiquated (e. Read More

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June 2009
1 Read

Prenatal diagnosis of juvenile granulosa cell tumor of the testis.

J Pediatr Urol 2008 Dec 28;4(6):472-4. Epub 2008 Aug 28.

Doernbecher Children's Hospital, Oregon Health and Science University, Division of Urology, Department of Surgery, 3303 Southwest Bond Avenue, 10th Floor, Portland, OR 97239, USA.

Juvenile granulosa cell tumor is a rare benign neoplasm of the testicular stroma that accounts for 1-5% of all prepubertal testis tumors [Metcalfe PD, Farivar-Mohseni H, Farhat W, McLorie G, Khoury A, Bagli DJ. Pediatric testicular tumors: contemporary incidence and efficacy of testicular preserving surgery. J Urol 2003;170:2412-2416; Ross JH, Rybicki L, Kay R. Read More

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December 2008
8 Reads

Increased androgen response to follicle-stimulating hormone administration in women with polycystic ovary syndrome.

J Clin Endocrinol Metab 2008 May 19;93(5):1827-33. Epub 2008 Feb 19.

Department of Reproductive Medicine, School of Medicine, University of California-San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0633, USA.

Context: In women with polycystic ovary syndrome (PCOS), excess ovarian androgen production is driven by increased LH secretion. Studies conducted in animals suggest that the granulosa cell may influence LH-stimulated theca cell androgen production.

Objective: The objective of this study was to determine whether FSH enhances androgen production in women with PCOS compared with that of normal women. Read More

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May 2008
5 Reads

Seasonal relationships between dopamine D1 and D2 receptor and equine FSH receptor mRNA in equine ovarian epithelium.

Anim Reprod Sci 2008 Oct 22;108(1-2):259-66. Epub 2007 Aug 22.

Department of Animal Science, Food and Nutrition, Southern Illinois University Carbondale, Mailcode 4417, 1205 Lincoln Drive, Carbondale, IL 62901, USA.

Dopamine (DA) blockade during anestrus or early spring transition can facilitate ovarian recrudescence and advance the timing of the first ovulation of the season. Some laboratories have reported variable results using DA antagonists to stimulate follicular growth during the mid-portion of the anestrual period. Differences in DA antagonist efficacy may be due to the FSH secretory status of the anestrous mare and the presence or absence of functional ovarian FSH receptors. Read More

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October 2008
1 Read

Bilateral occurrence of granulosa-theca cell tumors in an Arabian mare.

Can Vet J 2007 May;48(5):502-5

Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, North Carolina 27606, USA.

An Arabian mare was referred for right granulosa-theca cell tumor (GTCT) evaluation. The mare was presented 4.5 years later for a left GTCT, after successfully conceiving and delivering a normal foal in the interim. Read More

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May 2007
14 Reads

A granulosa-theca cell tumour in a 15-month-old Thoroughbred filly.

Aust Vet J 2007 Mar;85(3):124-5

Goulburn Valley Equine Hospital, Congupna VIC 3633, Australia.

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March 2007
4 Reads

Identification of a novel chondroitin-sulfated collagen in the membrane separating theca and granulosa cells in chicken ovarian follicles: the granulosa-theca cell interface is not a bona fide basement membrane.

J Biol Chem 2007 Mar 3;282(11):8011-8. Epub 2007 Jan 3.

Department of Medical Biochemistry, Max F. Perutz Laboratories, Medical University of Vienna, Dr. Bohr Gasse 9/2, A-1030 Vienna, Austria.

The membranous structure separating the granulosa from theca cells in the developing ovarian follicles of birds is generally perceived as a genuine basement membrane (BM). Previously, we suggested that this membrane is unusual in that it lacks several typical BM components, e.g. Read More

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March 2007
3 Reads

Occurrence of both breast and ovarian cancer in a woman is a marker for the BRCA gene mutations: a population-based study from western Sweden.

Fam Cancer 2007 ;6(1):35-41

Department of Oncology, Sahlgrenska University Hospital, S413 45 Göteborg, Sweden.

Aim: This study aimed to analyze whether the occurrence of both breast and ovarian cancer in a woman serves as a marker for BRCA gene mutations.

Material And Methods: This population-based study included 256 women in western Sweden who developed both invasive breast and ovarian tumors between 1958 and 1999. Archival paraffin tissue blocks of their tumors were retrieved for DNA-extraction to analyze the founder mutation, BRCA1 c. Read More

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September 2007
7 Reads

Gonadotropin secretion and pituitary responsiveness to GnRH in mares with granulosa-theca cell tumor.

Theriogenology 2006 Sep 2;66(5):1210-8. Epub 2006 May 2.

Department of Pathology, Diagnostic and Veterinary Clinic, Faculty of Veterinary Medicine, via S. Costanzo 4, 06126 Perugia, Italy.

Granulosa-theca cell tumors (GTCTs) are able to secrete variable amounts of sex steroids and immunoreactive inhibin (ir-INH). Although the pituitary appears to be affected by the presence of a GTCT, pituitary responsiveness to exogenous GnRH has not been examined. The aims of the present study were to: (i) assess the plasma hormone concentrations of ir-INH, gonadotropins and sex steroids in eight mares with GTCT and (ii) assess the responsiveness of pituitary gonadotroph cells to exogenous GnRH stimulus both before and after tumor removal. Read More

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September 2006
9 Reads

Diagnosis and surgical removal of a granulosa-theca cell tumor in a mare.

Kathy T Maurice

Can Vet J 2005 Jul;46(7):644-6

Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1.

An 8-year-old mare showed stallion-like behavior and aggressiveness. A granulosa-theca cell tumor was tentatively diagnosed based on history, transrectal palpation, and ultrasonography, and surgically removed. Serological levels of testosterone and inhibin were elevated. Read More

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July 2005
6 Reads

Granulosa theca cell tumor with luteoma in the ovary of a bonnet monkey (Macaca radiata).

J Med Primatol 2005 Aug;34(4):219-23

Primate Research center, National Institute of Immunology, New Delhi, India.

A mass was identified on the left caudal region of the abdomen in a 13-year-old bonnet monkey (Macaca radiata). The mass was excised and diagnosed as granulosa theca cell tumor accompanied with luteoma based on the microscopic findings. Morphologically it appeared pink, round, firm multilobulated measured approximately 5 x 3 x 2. Read More

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August 2005
8 Reads

Metabolic changes in pelvic lesions: findings at proton MR spectroscopic imaging.

Gynecol Obstet Invest 2005 25;60(3):121-7. Epub 2005 May 25.

Department of Obstetrics and Gynecology, Inonu University Medical Faculty, Malatya, Turkey.

Objective: The purpose of this study is to investigate the in vivo magnetic resonance spectroscopic (MRS) features of pelvic lesions using long echo time and to characterize the spectral patterns of various pathological entities.

Materials And Methods: 17 patients with surgically and histopathologically confirmed pelvic lesions underwent long echo-time MRS, and the results obtained were analyzed. Before laparotomy, choline (Cho), lactate, lipid and creatine (Cr) levels of all lesions were measured by single voxel MRS (point-resolved spectroscopy technique, TE 136 ms). Read More

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May 2006
7 Reads

Dopamine receptors in equine ovarian tissues.

Domest Anim Endocrinol 2005 May;28(4):405-15

Department of Animal Science, Food and Nutrition, Southern Illinois University, 1205 Lincoln Drive, MC 4417, Carbondale, IL 62901, USA.

Dopamine (DA) agonist and antagonist treatments can affect ovarian reproductive events in the mare. To support our theory that DA produces these effects by acting directly on the ovary, we analyzed equine ovarian tissues for the presence of dopamine receptor-1 (D1r) and dopamine receptor-2 (D2r) mRNA by reverse transcription polymerase chain reaction (RT-PCR) and D1r and D2r proteins by Western blot and immunohistochemistry (IHC). RT-PCR was performed on RNA isolated from ovarian cortex, medulla, granulosa/theca or corpus luteum (CL) tissues and from pituitary (D2r control) and renal artery (D1r control). Read More

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May 2005
1 Read

Inhibin alpha and beta expression in ovarian stromal tumors and their histological equivalences.

Acta Obstet Gynecol Scand 2004 May;83(5):491-6

Department of Pathology, Ege University Medical Faculty, 35100 Bornova, Izmir, Turkey.

Inhibin is a heterodimeric protein hormone that appears to be a sensitive immunohistochemical marker of sex cord-stromal tumors. Although sex cord-stromal tumors can usually be distinguished from ovarian epithelial tumors or their metastases by morphology or by using antibodies against intermediate filaments, the diagnosis remains difficult in rare situations in such cases as sarcomatoid granulosa-theca cell tumors, ovarian small cell carcinomas, or soft-tissue sarcomas. The purposes of this study were to examine inhibin alpha and beta immunoreactivity in a wide range of gonadal stromal neoplasms and to assess its value in the differential diagnosis of problematic tumors. Read More

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May 2004
7 Reads

Differential expression of interleukins IL-13 and IL-15 in normal ovarian tissue and ovarian carcinomas.

Gynecol Oncol 2004 Mar;92(3):761-8

Department of Obstetrics and Gynecology, University of Florida College of Medicine, Gainesville, FL 32610-0294, USA.

Objective: To determine the temporal and spatial expression of interleukins (IL)-13 and IL-15 in ovarian carcinoma compared to normal ovarian tissue.

Methods: Quantitative RT-PCR, ELISA and immunohistochemistry.

Results: Q-RT-PCR, ELISA and immunohistochemical analysis indicates that IL-13 and IL-15 mRNA and protein are expressed in normal ovary at various phases of the menstrual cycle with immunoreactive proteins detected in granulosa/theca and luteal cells and to a lesser extent in stromal cells and surface epithelial cells. Read More

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March 2004
4 Reads