89,560 results match your criteria Gram-Negative Folliculitis


Physicochemical responses and microbiological changes of bream (Megalobrama ambycephala) to pectin based coatings enriched with clove essential oil during refrigeration.

Int J Biol Macromol 2018 Dec 3. Epub 2018 Dec 3.

College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710062, PR China. Electronic address:

The effectiveness of pectin coatings enriched with clove essential oil (CEO), as new edible coatings were investigated to preserve bream (Megalobrama ambycephala) fillets during refrigeration over a period of 15 days. All samples were analyzed for physicochemical (pH, PV, TBA and TVB-N), microbiological (Total viable count, Psychrophilic bacteria, Lactic acid bacteria, Enterobacteriaceae, Pseudomonas spp., HS producing bacteria) and organoleptic attributes. Read More

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December 2018

The novel cationic cell-penetrating peptide PEP-NJSM is highly active against Staphylococcus epidermidis biofilm.

Int J Biol Macromol 2018 Dec 3. Epub 2018 Dec 3.

Laboratory of Molecular and Cellular Screening Processes, Centre of Biotechnology of Sfax, University of Sfax, Road Sidi Mansour km 6, PO BOX 1177, 3018 Sfax, Tunisia.

A cationic cell-penetrating peptide PEP-NJSM was identified in human virus proteomes by a screening of charge clusters in protein sequences generating cell penetrating peptides (CPP). PEP-NJSM was selectively active against Gram positive Staphylococcus epidermidis as antibacterial agent with MIC value of 128 μM compared to the Gram-negative Pseudomonas aeruginosa strain with MIC value exceeded 512 μM. The selected peptide exhibited an important anti-biofilm activity even at sub-MIC levels. Read More

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December 2018

Evaluation of the automated BD Phoenix CPO Detect panel in combination with the β-CARBA assay for detection and classification of carbapenemase-producing Enterobacterales.

J Microbiol Methods 2018 Dec 3. Epub 2018 Dec 3.

Institute of Clinical Microbiology and Hygiene, University Hospital of Regensburg, Franz-Josef-Strauß-Allee 11, D-93053 Regensburg, Germany.

Recently, the CPO Detect panel for the detection of carbapenemase-producing, Gram-negative bacteria was introduced for the Phoenix semi-automated antimicrobial susceptibility testing system. The CPO Detect assay aims to detect carbapenemase activity (P/N test) and to type carbapenemase producers according to the Ambler classification (Ambler test). The P/N test-based detection of carbapenemase producers was 100% sensitive and 55. Read More

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December 2018
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Molecular optimization of autotransporter-based tyrosinase surface display.

Biochim Biophys Acta Biomembr 2018 Dec 3. Epub 2018 Dec 3.

Department of Industrial Biotechnology, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, AlbaNova University Center, SE 10691 Stockholm, Sweden. Electronic address:

Display of recombinant enzymes on the cell surface of Gram-negative bacteria is a desirable feature with applications in whole-cell biocatalysis, affinity screening and degradation of environmental pollutants. One common technique for recombinant protein display on the Escherichia coli surface is autotransport. Successful autotransport of an enzyme largely depends on the following: (1) the size, sequence and structure of the displayed protein, (2) the cultivation conditions, and (3) the choice of the autotransporter expression system. Read More

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December 2018

Acquisition and transfer of antibiotic resistance genes in association with conjugative plasmid or class 1 integrons of Acinetobacter baumannii.

PLoS One 2018 6;13(12):e0208468. Epub 2018 Dec 6.

Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand.

Conjugation is a type of horizontal gene transfer (HGT) that serves as the primary mechanism responsible for accelerating the spread of antibiotic resistance genes in Gram-negative bacteria. The present study aimed to elucidate the mechanisms underlying the conjugation-mediated gene transfer from the extensively drug-resistant Acinetobacter baumannii (XDR-AB) and New Delhi Metallo-beta-lactamase-1-producing Acinetobacter baumannii (NDM-AB) to environmental isolates of Acinetobacter spp. Conjugation experiments demonstrated that resistance to ticarcillin and kanamycin could be transferred from four donors to two sodium azide-resistant A. Read More

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December 2018

Bacterial microbiome of the chigger mite Leptotrombidium imphalum varies by life stage and infection with the scrub typhus pathogen Orientia tsutsugamushi.

PLoS One 2018 6;13(12):e0208327. Epub 2018 Dec 6.

Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, North Carolina, United States of America.

Scrub typhus is a mites-borne rickettsiosis caused by the obligate intracellular Gram-negative bacterium Orientia tsutsugamushi. The disease is potentially life threatening and is prevalent in tropical Asia, islands of the western Pacific Ocean and northern Australia where an estimated one million cases occur annually. Orientia tsutsugamushi is transmitted by the bite of larval mites in the genus Leptotrombidium. Read More

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December 2018

The influence of different composite mixtures (PLA/HA) manufactured with additive laser technology on the ability of S. aureus and P. aeruginosa to form biofilms.

Acta Bioeng Biomech 2018 ;20(4):101-106

Wrocław University of Science and Technology, Wrocław, Poland.

Purpose: Staphylococcus aureus (Gram-positive coccus) and Pseudomonas aeruginosa (Gram-negative bacterium) are the leading etiologic agents of biofilm-related, life-threatening infections in patients after orthopaedic implantations. The aim of the present paper is to estimate the ability of these two bacterial strains to form a biofilm on bioresorbable composites manufactured from polylactide (PLA) and hydroxyapatite (HA) with the use of Selective Laser Sintering (SLS) method.

Methods: Microbiological tests were conducted on two variants of a solid specimen made with additive laser technology. Read More

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January 2018

Synthesis, Characterization, Antibacterial and Antioxidant Potency of N-Substituted-2-Sulfanylidene-1,3-Thiazolidin-4-one Derivatives and QSAR Study.

Med Chem 2018 Dec 5. Epub 2018 Dec 5.

Faculty of Agrobiotehnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, Osijek. Croatia.

Background: Rhodanine is known for its potential and important role in the medicinal chemistry since its derivatives exhibit a wide range of pharmacological activities such as antibacterial, antifungal, antidiabetic, antitubercular, anti-HIV, antiparasitic, antioxidant, anticancer, anti-proliferative and anthelmintic agents.

Objectives: Since N-substituted rhodanine synthons are rarely commercially available, it is desirable to develop a straight forward synthetic approach for the synthesis of these key building blocks. The objective was to synthesize a series of rhodanine derivatives and to investigate their antimicrobial and antioxidant activity. Read More

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December 2018

Winogradskyella algae sp. nov., a marine bacterium isolated from the brown alga.

Antonie Van Leeuwenhoek 2018 Dec 5. Epub 2018 Dec 5.

G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, Vladivostok, Russia, 690022.

An aerobic, Gram-negative, yellow-pigmented non-motile rod-shaped bacterium Kr9-9 was isolated from a brown alga specimen collected near the Kuril Islands. Based on the 16S rRNA gene sequence analysis strain Kr9-9 was assigned to the genus Winogradskyella, and its close phylogenetic neighbors were found to be Winogradskyella damuponensis KCTC 23552, Winogradskyella sediminis LMG 28075, and Winogradskyella rapida CCUG 59098 showing high similarities of 98.1%, 97. Read More

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December 2018
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Zinc Blockade of SOS Response Inhibits Horizontal Transfer of Antibiotic Resistance Genes in Enteric Bacteria.

Front Cell Infect Microbiol 2018 21;8:410. Epub 2018 Nov 21.

Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, United States.

The SOS response is a conserved response to DNA damage that is found in Gram-negative and Gram-positive bacteria. When DNA damage is sustained and severe, activation of error-prone DNA polymerases can induce a higher mutation rate than is normally observed, which is called the SOS mutator phenotype or hypermutation. We previously showed that zinc blocked the hypermutation response induced by quinolone antibiotics and mitomycin C in and . Read More

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November 2018
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Draft genomes of strains isolated from dried spices bring unique insights into the diversity of plant-associated strains.

Stand Genomic Sci 2018 29;13:35. Epub 2018 Nov 29.

1Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration, 8301 Muirkirk Road, Laurel, MD 20708 USA.

is a Gram-negative opportunistic pathogen that causes life- threatening infantile infections, such as meningitis, septicemia, and necrotizing enterocolitis, as well as pneumonia, septicemia, and urinary tract and wound infections in adults. Here, we report 26 draft genome sequences of , which were obtained from dried spices from the USA, the Middle East, China, and the Republic of Korea. The average genome size of the genomes was 4393 kb, with an average of 4055 protein coding genes, and an average genome G + C content of 56. Read More

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November 2018
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Opportunistic Bacteria Dominate the Soil Microbiome Response to Phenanthrene in a Microcosm-Based Study.

Front Microbiol 2018 21;9:2815. Epub 2018 Nov 21.

Microbiology, School of Natural Sciences, Ryan Institute, National University of Ireland, Galway, Ireland.

Bioremediation offers a sustainable approach for removal of polycyclic aromatic hydrocarbons (PAHs) from the environment; however, information regarding the microbial communities involved remains limited. In this study, microbial community dynamics and the abundance of the key gene (PAH-RHDα) encoding a ring hydroxylating dioxygenase involved in PAH degradation were examined during degradation of phenanthrene in a podzolic soil from the site of a former timber treatment facility. The 10,000-fold greater abundance of this gene associated with Gram-positive bacteria found in phenanthrene-amended soil compared to unamended soil indicated the likely role of Gram-positive bacteria in PAH degradation. Read More

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November 2018

SuperPolymyxin™ Medium for the Screening of Colistin-Resistant Gram-Negative Bacteria in Stool Samples.

Front Microbiol 2018 21;9:2809. Epub 2018 Nov 21.

Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.

Colistin is one of the last resort antimicrobials for the treatment of infections caused by multidrug-resistant Gram-negative bacteria. After the emergence of transferable colistin resistance genes (-1-5), a reliable culture-based screening method to detect colonization with colistin-resistant Gram-negative bacteria (CRGN) is needed. The objective of this study was to test the performance of SuperPolymyxin™ medium to screen for CRGN in stool samples and to compare different methods for the confirmation of colistin resistance (e. Read More

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November 2018

The Outer Membrane Vesicles of ATCC 7966: A Proteomic Analysis and Effect on Host Cells.

Front Microbiol 2018 16;9:2765. Epub 2018 Nov 16.

Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico.

Gram-negative bacteria release outer membrane vesicles (OMVs) into the extracellular environment. OMVs have been studied extensively in bacterial pathogens, however, information related with the composition of OMVs is missing. In this study we analyzed the composition of purified OMVs from ATCC 7966 by proteomics. Read More

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November 2018
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A case of ulceroglandular tularemia presenting with lymphadenopathy and an ulcer on a linear morphoea lesion surrounded by erysipelas.

Int Med Case Rep J 2018 12;11:313-318. Epub 2018 Nov 12.

Department of Infectious Diseases, Regional Hospital of Bellinzona e Valli, 6500 Bellinzona, Ticino, Switzerland.

Tularemia is a zoonosis caused by the infection of (a gram-negative aerobic bacterium). Transmission to other animals or humans usually occurs through insect or tick bites, direct contact with a contaminated environment (mud or water), infected animals - mainly lagomorphs - or by ingesting undercooked meat or inhaling contaminated dust (hay or soil). This paper discusses the case of a 32-year-old man, who came to our Emergency Room presenting with persistent fever, inguinal lymphadenopathy, and an ulcer on his left lower limb on a linear morphoea lesion that had been there for some time. Read More

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November 2018

Distribution and drug resistance of pathogenic bacteria in ventilator-associated pneumonia at a local hospital of North-eastern China.

Infect Drug Resist 2018 13;11:2249-2255. Epub 2018 Nov 13.

Department of Emergency, Shengjing Hospital Affiliated to China Medical University, Heping District, Shenyang, Liaoning Province 110004, People's Republic of China,

Purpose: To study the distribution of pathogenic bacteria in ventilator-associated pneumonia (VAP), and epidemiological characteristics of extended-spectrum β lactamase (ESBL)-producing bacteria.

Patients And Methods: Lower respiratory tract secretions from 156 patients with mechanical ventilation were collected using a protective specimen brush (PSB), with quantitative bacterial culture carried out and antibiotic sensitivity measured. ESBLs produced by Gram-negative bacilli were detected using the double disk diffusion method and monitored by plasmid profiles. Read More

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November 2018

Emergence of tigecycline resistance in co-producing MCR-1 and NDM-5 during tigecycline salvage treatment.

Infect Drug Resist 2018 13;11:2241-2248. Epub 2018 Nov 13.

Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, China,

Objective: Here, we report a case of severe infection caused by that harbored , , and acquired resistance to tigecycline during tigecycline salvage therapy.

Methods: Antimicrobial susceptibility testing, Southern blot hybridization, and complete genome sequence of the strains were carried out. The genetic characteristics of the and plasmids were analyzed. Read More

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November 2018

Inhibition of inflammasome activation by a clinical strain of Klebsiella pneumoniae impairs efferocytosis and leads to bacterial dissemination.

Cell Death Dis 2018 Dec 5;9(12):1182. Epub 2018 Dec 5.

Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University, Araraquara, São Paulo, Brazil.

Klebsiella pneumoniae is a Gram-negative bacterium responsible for severe cases of nosocomial pneumonia. During the infectious process, both neutrophils and monocytes migrate to the site of infection, where they carry out their effector functions and can be affected by different patterns of cell death. Our data show that clinical strains of K. Read More

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December 2018

A systematic review of the association between delayed appropriate therapy and mortality among patients hospitalized with infections due to Klebsiella pneumoniae or Escherichia coli: how long is too long?

BMC Infect Dis 2018 Dec 5;18(1):625. Epub 2018 Dec 5.

Meta Research, Evidera, Bethesda, MD, USA.

Background: Temporal relationships between the time to appropriate antibiotic therapy and outcomes are not well described.

Methods: A systematic literature review and meta-analysis was performed to examine this relationship in patients hospitalized with Klebsiella pneumoniae or Escherichia coli infections.

Results: Twenty identified studies contained data for patients who received delayed appropriate therapy (DAT) versus appropriate antibiotic therapy for these pathogens. Read More

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December 2018

The Yersinia pseudotuberculosis Cpx envelope stress system contributes to transcriptional activation of rovM.

Virulence 2018 Dec 5. Epub 2018 Dec 5.

a Department of Molecular Biology , Umeå University , Umeå , Sweden.

The Gram-negative enteropathogen Yersinia pseudotuberculosis possesses a number of regulatory systems that detect cell envelope damage caused by noxious extracytoplasmic stresses. The CpxA sensor kinase and CpxR response regulator two-component regulatory system is one such pathway. Active Cpx signalling upregulates various factors designed to repair and restore cell envelope integrity. Read More

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December 2018
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Antibacterial and anti-biofilm activity, and mechanism of action of pleurocidin against drug resistant Staphylococcus aureus.

Microb Pathog 2018 Nov 30. Epub 2018 Nov 30.

Department of Biomedical Science, Chosun University, Gwangju, 61452, South Korea; Research Center for Proteineous Materials, Chosun University, Gwangju, 61452, South Korea. Electronic address:

The abuse of antibiotics has resulted in the emergence of multi-drug-resistant bacteria. Staphylococcus aureus is a frequent cause of infections, and antibiotic-resistant S. aureus has become a serious problem. Read More

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November 2018

Comparative analysis of the antibacterial and drug-modulatory effect of d-limonene alone and complexed with β-cyclodextrin.

Eur J Pharm Sci 2018 Nov 30. Epub 2018 Nov 30.

Department of Biological Chemistry, Laboratory of Microbiology and Molecular Biology - LMBM, Regional University of Cariri, Crato, Ceará, Brazil. Electronic address:

With the increase in bacterial resistance to antibiotics, many studies have been directed towards finding new agents with antibacterial activity, such as studies with natural products. These products can have antibacterial activity such as d-limonene as described in the literature. The aim of this study was to evaluate the antibacterial activity of d-limonene, isolated and complexed with β-cyclodextrin, and to evaluate its potentiating activity of different antibiotic classes. Read More

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November 2018

Performances of the Rapid Polymyxin Acinetobacter and Pseudomonas Tests for Colistin Susceptibility Testing.

Microb Drug Resist 2018 Dec 1. Epub 2018 Dec 1.

1 Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, Faculty of Science and Medicine, University of Fribourg , Fribourg, Switzerland .

Objectives: Owing to the emergence of colistin resistance in nonfermenting Gram negative bacteria, reliable and rapid techniques for testing colistin susceptibility are needed. We evaluated the performances of the Rapid Polymyxin Acinetobacter and Pseudomonas tests using a collection of Acinetobacter baumannii and Pseudomonas aeruginosa clinical isolates.

Methods: Colistin susceptibility of A. Read More

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December 2018

A novel cfr-carrying Tn7 transposon derivative characterized in Morganella morganii of swine origin in China.

J Antimicrob Chemother 2018 Nov 30. Epub 2018 Nov 30.

Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan Province, People's Republic of China.

Objectives: To characterize the presence and genetic environment of the multiresistance gene cfr in bacterial isolates from a swine farm.

Methods: A total of 97 bacterial isolates, recovered from 32 faecal swabs obtained on one farm, were tested for the presence of the cfr gene by PCR. Species identification of the one cfr-positive strain was conducted using the BD PhoenixTM 100 Automated Microbiology System. Read More

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November 2018

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment.

J Vis Exp 2018 Nov 17(141). Epub 2018 Nov 17.

The Peter Gorer Department of Immunobiology, King's College London; The Francis Crick Institute;

The gut microbiota has a central influence on human health. Microbial dysbiosis is associated with many common immunopathologies such as inflammatory bowel disease, asthma and arthritis. Thus, understanding the mechanisms underlying microbiota-immune system crosstalk is of crucial importance. Read More

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November 2018

Bacterial sepsis triggers an antiviral response that causes translation shutdown.

J Clin Invest 2018 Dec 3. Epub 2018 Dec 3.

Department of Medicine.

In response to viral pathogens, the host upregulates antiviral genes that suppress translation of viral mRNAs. However, induction of such antiviral responses may not be exclusive to viruses, as the pathways lie at the intersection of broad inflammatory networks that can also be induced by bacterial pathogens. Using a model of Gram-negative sepsis, we show that propagation of kidney damage initiated by a bacterial origin ultimately involves antiviral responses that result in host translation shutdown. Read More

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December 2018

Induction Effects of Bacteroides fragilis Derived Outer Membrane Vesicles on Toll Like Receptor 2, Toll Like Receptor 4 Genes Expression and Cytokines Concentration in Human Intestinal Epithelial Cells.

Cell J 2019 Apr 18;21(1):57-61. Epub 2018 Nov 18.

Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.

Objective: Gastrointestinal (GI) tract, like other mucosal surface, is colonized with a microbial population known as gut microbiota. Outer membrane vesicles (OMVs) which are produced by gram negative bacteria could be sensed by Toll like receptors (TLRs). The interaction between gut microbiota and TLRs affects homeostasis and immune responses. Read More

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Detection of Mycoplasma Contamination of Cell Culture by A Loop-Mediated Isothermal Amplification Method.

Cell J 2019 Apr 18;21(1):43-48. Epub 2018 Nov 18.

Tasnim Biotechnology Research Center (TBRC), Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran.

Objective: Mycoplasmas are major contaminants of cell culture and affect in vitro biological and diagnostic tests. Mycoplasma detection is conducted using culture and molecular methods. These methods vary in terms of accuracy, reliably and sensitivity. Read More

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April 2019
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Synthesis and biological profile of substituted benzimidazoles.

Chem Cent J 2018 Dec 1;12(1):125. Epub 2018 Dec 1.

Department of Pharmacology and Toxicology, College of Pharmacy, Qassim University, Buraidah, 51452, Kingdom of Saudi Arabia.

Background: A series of benzimidazole derivatives was developed and its chemical scaffolds were authenticated by NMR, IR, elemental analyses and physicochemical properties. The synthesized compounds were screened for their antimicrobial and antiproliferative activities.

Results And Discussion: The synthesized benzimidazole compounds were evaluated for their antimicrobial activity using the tube dilution method and were found to exhibit good antimicrobial potential against selected Gram negative and positive bacterial and fungal species. Read More

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December 2018
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Cloning and Expression of Lipopolysaccharide Elimination Protein (LEP) in Lactic Acid Bacteria.

Methods Mol Biol 2019 ;1887:175-187

Department of Food Management, Miyagi University, Sendai, Miyagi, Japan.

Lipopolysaccharide (LPS) is related to human inflammation. Therefore, in the probiotics research field, controlling the mechanisms of LPS neutralization and elimination of inflammation of human intestines are important. This chapter presents a description of the identification of LPS elimination protein (LEP) in lactic acid bacteria (LAB), cloning of its protein, and its expression. Read More

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January 2019

Eliminating Lipopolysaccharide (LPS) Using Lactic Acid Bacteria (LAB) and a Fraction of its LPS-Elimination Protein.

Methods Mol Biol 2019 ;1887:167-174

Department of Food Management, Miyagi University, Sendai, Miyagi, Japan.

Lactic acid bacteria (LAB) can improve human intraintestinal conditions. One reason is that ingestion of LAB prevents bacterial diarrhea. Furthermore, inflammation of human intestines can be caused by a lipopolysaccharide (LPS) component in the cell walls of gram-negative bacteria. Read More

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January 2019

A presentation of culture-positive corneal donors and the effect on clinical outcomes.

Graefes Arch Clin Exp Ophthalmol 2018 Nov 30. Epub 2018 Nov 30.

Department of Ophthalmology, Rigshospitalet-Glostrup, University of Copenhagen, Glostrup, Denmark.

Purpose: Donor-to-host transmission of infectious agents is a rare but well-recognised complication of corneal transplantation and may carry a grave visual prognosis. In this case series, we describe the clinical features and risk factors of using culture-positive donor corneas for transplantation.

Methods: Retrospective chart review of a series of patients who underwent either penetrating keratoplasty (PK) or Descemet's stripping automated endothelial keratoplasty (DSAEK) with positive microbiology cultivation during routine assessment of donor corneal tissue obtained at the time of surgery. Read More

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November 2018

Pulmonary Valve Prosthesis Endocarditis Caused By .

Thorac Cardiovasc Surg Rep 2018 Jan 29;7(1):e47-e49. Epub 2018 Nov 29.

Center of Infectious Diseases, Heidelberg University Hospital, Heidelberg, Germany.

  is a gram-negative bacterium assigned to the family of . Less than 1% of Q-fever infection leads to infective endocarditis (IE). Cases of reported pulmonary valve (PV) prosthesis endocarditis are scarce. Read More

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January 2018

High-quality-draft genome sequence of the heavy metal resistant and exopolysaccharides producing bacterium TBZ30.

Stand Genomic Sci 2018 28;13:34. Epub 2018 Nov 28.

State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070 People's Republic of China.

TBZ30 (= CCTCC AB 2015301 = KCTC 42833) is a Gram- negative, rod-shaped, non-motile and non-spore-forming bacterium isolated from a heavy metal contaminated paddy field. It shows resistance to multiple heavy metals and can adsorb/remove Zn and Cd during cultivation. In addition, strain TBZ30 produces exopolysaccharides (EPS). Read More

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November 2018

The epidemiology of nonviral microbial keratitis in a tertiary care center in Muscat, Oman.

Oman J Ophthalmol 2018 Sep-Dec;11(3):213-219

Department of Ophthalmology, Al-Nahdah Hospital, Muscat, Oman.

Objective: The objective of this study is to describe the epidemiology, risk factors, etiology, and outcome of microbial keratitis in a tertiary care center in Muscat, Oman.

Methods: The electronic records of all patients diagnosed with keratitis or corneal ulcer between January 2013 and January 2016 in Al-Nahdha Hospital were retrospectively reviewed. Patients who presented with a corneal ulcer requiring admission and who underwent culture and sensitivity studies were included in the study. Read More

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December 2018

Administration of antibiotics contributes to cholestasis in pediatric patients with intestinal failure via the alteration of FXR signaling.

Exp Mol Med 2018 Nov 30;50(12):155. Epub 2018 Nov 30.

Department of Pediatric Surgery, Xin Hua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

The link between antibiotic treatment and IF-associated liver disease (IFALD) is unclear. Here, we study the effect of antibiotic treatment on bile acid (BA) metabolism and investigate the involved mechanisms. The results showed that pediatric IF patients with cholestasis had a significantly lower abundance of BA-biotransforming bacteria than patients without cholestasis. Read More

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November 2018

Isolation of a Capnophilic and an Extended-Spectrum-β-Lactamase-Producing Proteus mirabilis Isolate with Inability to Grow under pH-Indicator Dyes from the Urine of an Octogenarian Male-Patient with Acute Pyelonephritis.

Jpn J Infect Dis 2018 Nov 30. Epub 2018 Nov 30.

Department of Health and Medical Sciences, Shinshu University Graduate School of Medicine.

A capnophilic Gram-negative rod was recovered from the urine of an octogenarian male patient with acute pyelonephritis. The isolate also demonstrated to produce CTX-M-2-type- extended-spectrum-β-lactamase. Interestingly, the isolate failed to grow on modified Drigalski (BTB) and MacConkey agars even in the CO-enriched atmosphere. Read More

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November 2018

virulence factors, including Listeriolysin O, are secreted in biologically active Extracellular Vesicles.

J Biol Chem 2018 Nov 30. Epub 2018 Nov 30.

Johns Hopkins University, United States.

Outer membrane vesicles produced by Gram-negative bacteria have been studied for half a century but the possibility that Gram-positive bacteria secreted extracellular vesicles (EVs) was not pursued until recently due to the assumption that the thick peptidoglycan cell wall would prevent their release to the environment. However, following their discovery in fungi, which also have cell walls, EVs have now been described for a variety of Gram-positive bacteria. EVs purified from Gram-positive bacteria are implicated in virulence, toxin release and transference to host cells, eliciting immune responses, and spread of antibiotic resistance. Read More

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November 2018
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Attributable Clinical and Economic Burden of Carbapenem-Nonsusceptible Gram-Negative Infections in Patients Hospitalized With Complicated Urinary Tract Infections.

J Hosp Infect 2018 Nov 29. Epub 2018 Nov 29.

Merck & Co., Inc., Kenilworth, NJ, USA. Electronic address:

Background: Gram-negative complicated urinary tract infections (cUTIs) can have serious consequences for patients and hospitals.

Aim: We examined the clinical and economic burden attributable to Gram-negative carbapenem-nonsusceptible (C-NS; resistant/intermediate) versus carbapenem-susceptible (C-S) infections in 78 United States (US) hospitals.

Methods: All nonduplicate C-NS and C-S urine source isolates were analyzed. Read More

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November 2018

MS2-Affinity Purification Coupled With RNA Sequencing Approach in the Human Pathogen Staphylococcus aureus.

Methods Enzymol 2018 25;612:393-411. Epub 2018 Sep 25.

Architecture et Réactivité de l'ARN, Université de Strasbourg, IBMC-CNRS, Strasbourg, France. Electronic address:

Staphylococcus aureus is a Gram-positive major human pathogen involved in a wide range of human infectious diseases (from minor skin infections to septicemia, endocarditis or toxic shock syndrome). The treatment of S. aureus infections is very challenging due to the emergence of multiple antibiotic-resistant isolates. Read More

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September 2018

Analysis of enzyme activity, antibacterial activity, antiparasitic activity and physico-chemical stability of skin mucus derived from Amphiprion clarkii.

Fish Shellfish Immunol 2018 Nov 28. Epub 2018 Nov 28.

State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, Fujian Province, China; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, 361102, Fujian Province, China; State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, Fujian Province, China. Electronic address:

Recently, mucosal surfaces, especially fish skin and its secreted mucus, have attracted significant interest from immunologists. Amphiprion clarkii, a member of the family Pomacentridae, lives symbiosis with sea anemones and has a good resistance to common seawater bacterial diseases and parasites owing to the protection from its abundant skin mucus. In the present work, the activity of immune-related enzymes (lysozyme, protease, antiprotease, cathepsin B, alkaline phosphatase and peroxidase), the antibacterial activity against two Gram-positive bacteria and five Gram-negative bacteria, the antiparasitic activity against the pathogen of marine white spot disease (Cryptocaryon irritans theronts) and the physico-chemical stability (to pH and heat) of the skin mucus of A. Read More

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November 2018

Hybrid Antibacterial Nanocomposites Based on the Vermiculite/Zinc Oxide-Chlorhexidine.

J Nanosci Nanotechnol 2019 May;19(5):3041-3048

Nanotechnology Centre, VŠ B-Technical University of Ostrava, 708 33 Ostrava-Poruba, Czech Republic Š.

The hybrid nanocomposite materials based on the vermiculite/zinc oxide-chlorhexidine were prepared in two steps. In the first step the vermiculite/zinc oxide nanocomposite was prepared by the mechanochemical method followed by a heat treatment at 650 °C for 90 min. In the second step the chlorhexidine dihydrochloride was intercalated to the vermiculite/zinc oxide nanocomposite in weight ratio 1:1, 1:2, 1:4, 2:1 and 4:1 (wt%) thereby vermiculite/zinc oxide-chlorhexidine nanocomposites were prepared. Read More

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Enhanced Antibacterial Activity and Photo-Catalytic Properties of ZnO Nanoparticles: Plant Extract-Assisted Synthesis.

J Nanosci Nanotechnol 2019 May;19(5):2888-2894

Department of Chemistry, Urumu Dhanalakshmi College, Tiruchirappalli 620019, Tamil Nadu, India.

Semiconductor ZnO samples with two different morphologies such as nanoparticles (ZnO-NPs) and nano-sheets (ZnO-NSs) was successfully prepared by microwave heating (MHM) and modified sol-gel (SGM) method, respectively using plant extract as the bio-reducing agent. Structural, purity and morphology of the samples was examined by X-ray powder diffraction (XRD), scanning electron microscopy (SEM) with energy dispersive X-ray (EDX) analysis. Optical property analysis and band gap calculation were carried out by UV-Visible diffuse reflectance (DRS) and photoluminescence (PL) spectroscopy techniques. Read More

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Antioxidant and Photocatalytic Activity of Aqueous Leaf Extract Mediated Green Synthesis of Silver Nanoparticles Using .

J Nanosci Nanotechnol 2019 May;19(5):2640-2648

UNESCO-UNISA Africa Chair in Nanosciences/Nanotechnology Laboratories, College of Graduate Studies, University of South Africa (UNISA), Muckleneuk Ridge, P. O. Box 392, Pretoria 001, South Africa.

The purpose of this study is to minimize the negative impacts of synthetic procedures and to develop environmentally benign procedures for the synthesis of metallic nanoparticles. In the present study, () aqueous leaf extract mediated green synthesis of silver nanoparticles are described. The synthesized silver nanoparticles were characterized by UV-Vis Spectroscopy, Fluorescence Spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD), Scanning Electron Microscopy (SEM) Transmission Electron Microscopy (TEM) and Cyclic Voltammetry (CV). Read More

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Polyethylene/Organo-Inorgano Vermiculites and Their Antimicrobial Properties.

J Nanosci Nanotechnol 2019 May;19(5):2599-2605

SPUR a.s., těr. T. Bati 299, 764 22 Zlín, Czech Republic.

Clay mineral vermiculite was treated with silver and copper nitrate solutions and samples were subsequently modified with organic compound (dodecylamine) via solid-solid melt intercalation. Prepared organo-inorgano vermiculites were used as nanofillers to the polyethylene matrix. Mixtures of polyethylene with vermiculite nanofillers, prepared by melt compounding technique, were pressed into thin plates. Read More

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Routine Diagnostic Tests for Periprosthetic Joint Infection Demonstrate a High False-Negative Rate and Are Influenced by the Infecting Organism.

J Bone Joint Surg Am 2018 Dec;100(23):2057-2065

The Rothman Institute at Thomas Jefferson University, Philadelphia, Pennsylvania.

Background: Current guidelines recommend serum erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) as the first-line testing for evaluation of suspected periprosthetic joint infection, in addition to synovial white blood-cell (WBC) count and polymorphonuclear percentage. However, the sensitivity and other diagnostic measures of these tests using a standardized definition of periprosthetic joint infection and the influence of organisms on these inflammatory markers remain inadequately investigated.

Methods: A retrospective review of an institutional database of 549 periprosthetic joint infection cases and 653 aseptic total joint arthroplasty revisions was performed. Read More

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December 2018
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Etiology of Childhood Otorrhea in Luanda, Angola, and a Review of Otitis Media in African Children.

Pediatr Infect Dis J 2018 Dec 3. Epub 2018 Dec 3.

Children's Hospital, Helsinki University Hospital, Helsinki, Finland and University of Helsinki, Helsinki, Finland.

Background: In resource-poor settings, otorrhea causes a significant burden of disease in children. Etiologic studies and structured data on otorrhea and chronic otitis media among African children remain scarce.

Methods: Here, we reviewed 678 bacteriologically analyzed otorrhea samples from Luanda Children's Hospital from children ≤15 years of age between 2008 and 2015. Read More

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December 2018
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Susceptibility of Tc-Ciprofloxacin for Common Infection Causing Bacterial Strains Isolated from Clinical Samples: an In Vitro and In Vivo Study.

Appl Biochem Biotechnol 2018 Dec 5. Epub 2018 Dec 5.

Department of Chemistry, Government College University, New Campus, Faisalabad, 38000, Pakistan.

Tc-ciprofloxacin scintigraphy is useful in the detection of gram-positive and gram-negative bacterial infections and also for differentiating the infection from aseptic inflammation. However, due to growing bacterial resistance to antibiotics, the Tc-ciprofloxacin no longer can be effective in broad-spectrum infection imaging as it is gradually losing specificity. In this study, we are presenting our findings regarding the in vitro and in vivo susceptibility of Tc-ciprofloxacin for multi-drug-resistant Staphylococcus aurous, Escherichia coli, and Pseudomonas aeruginosa bacterial strains which were isolated from clinical samples. Read More

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December 2018

Purification and Characterization of Glutathione Binding Protein GsiB from .

Biomed Res Int 2018 1;2018:3429569. Epub 2018 Nov 1.

Department of Gynecology, Central Hospital of Xuzhou, Affiliated Hospital of Southeast University, Xuzhou, China.

Objectives: To purify and characterize the glutathione binding protein GsiB of glutathione importer (GSI) in .

Results: The coding sequence of GsiB was cloned from MG1655 and expressed in BL21(DE3). GsiB protein was expressed and purified to homogeneity using Ni-affinity and gel filtration chromatography. Read More

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November 2018

Isolation of from Gastric Biopsy of Dyspeptic Patients in Ghana and Preliminary Assessment of the Effect of Extract on Its Growth.

J Trop Med 2018 31;2018:8071081. Epub 2018 Oct 31.

Department of Molecular Biology and Biotechnology, School of Biological Sciences, College of Agriculture and Natural Sciences, University of Cape Coast, Cape Coast, Ghana.

() is a gram-negative bacterium that colonizes the human stomach. Infection with this microaerophilic bacterium causes gastric and duodenal ulcer. This study sought to isolate , from gastric biopsy samples of dyspeptic patients in Ghana using a 2,3,5-triphenyltetrazolium chloride (TTC) dye incorporated medium method. Read More

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October 2018