12,726 results match your criteria Gonococcus


How actively should we screen for chlamydia and gonorrhoea in MSM and other high-ST-prevalence populations as we enter the era of increasingly untreatable infections? A viewpoint.

Authors:
Chris Kenyon

J Med Microbiol 2018 Dec 6. Epub 2018 Dec 6.

2​Division of Infectious Diseases and HIV Medicine, University of Cape Town, Anzio Road, Observatory 7700, South Africa.

A number of national and international organizations are advocating more intensive screening for Chlamydia trachomatis and Neisseria gonorrhoeae in high-prevalence populations as a way to reduce the prevalence of these infections. In this article, we review the available evidence and conclude that there is a paucity of evidence to support this approach. We further hypothesize that increasing screening intensity in high-prevalence populations will result in a considerable risk for the emergence of antimicrobial resistance in Neisseria gonorrhoeae and other pathobionts. Read More

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December 2018

Contraceptive use and the risk of sexually transmitted infection: systematic review and current perspectives.

Open Access J Contracept 2018 12;9:91-112. Epub 2018 Nov 12.

Global Health, Population and Nutrition, FHI 360, Durham, NC, USA,

Purpose: Evidence on the association between contraceptive use and risk of sexually transmitted infections (STIs) and bacterial vaginosis (BV) is lacking, with few prospective studies. We systematically reviewed the last 10 years' evidence on the association between contraception and STI/BV, building on the most recent systematic reviews published in 2006 and 2009.

Methods: We searched the MEDLINE and POPLINE databases for peer-reviewed articles p ublished between January 1, 2008 and January 31, 2018 reporting prospective studies that assessed the association between contraceptive use and incident STI and/or incident or recurrent BV. Read More

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November 2018

Adult conjunctivitis secondary to dual infection with and - A case report.

Am J Ophthalmol Case Rep 2019 Mar 14;13:6-8. Epub 2018 Nov 14.

Manchester Royal Eye Hospital, Oxford Road, Manchester, M13 9WL, UK.

Purpose: Although and are the commonest sexually transmitted infections in England, reports of ocular co-infection in the literature are limited. We report such a case which responded well to treatment, and discuss the literature and evidence currently available with regards to management of these cases.

Observations: The patient is a 48-year-old bisexual gentleman who presented to the eye clinic of a UK hospital with redness, discharge and blurred vision in his left eye for one week. Read More

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Prediction of epitopes of Neisseria Gonorrhoeae against USA human leukocyte antigen background: An immunoinformatic approach towards development of future vaccines for USA population.

Mol Cell Probes 2018 Nov 28. Epub 2018 Nov 28.

Department of Biochemistry and Biotechnology, University of Gujrat, P. O Box No, 50700, Pakistan. Electronic address:

Gonorrheal infections are the second most prevalent sexually transmitted diseases STDs in the USA populations after Chlamydia. These infections have now become an urgent problem to address because Neisseria gonorrhoeae is capable of developing resistance to multiple antibiotic classes. Infection with these antibiotic-resistant strains has become the major public health concern. Read More

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November 2018

Prevalence of Ureaplasma urealyticum, Chlamydia trachomatis, Neisseria gonorrhoeae and herpes simplex virus in Beijing, China.

Epidemiol Infect 2018 Dec 3:1-5. Epub 2018 Dec 3.

Department of Infection Control,Affiliated Hospital of Academy of Military Medical Sciences,Beijing,China.

The prevalence of sexually transmitted infection (STI) pathogens in Beijing, China, is rarely reported. In this study, 34 911 symptomatic outpatients with suspected genital infections who attended outpatient clinics in a tertiary care hospital were included to investigate the updated prevalence of Ureaplasma urealyticum (UU), Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG) and herpes simplex virus (HSV) from 1 January 2013 to 31 December 2016 in Beijing, China. Results indicated that a decrease trend (UU, CT, NG and HSV) in male and an increase trend (UU, CT and NG) in female were observed during the period. Read More

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December 2018

The MtrCDE Efflux Pump Contributes to Survival of From Human Neutrophils and Their Antimicrobial Components.

Front Microbiol 2018 20;9:2688. Epub 2018 Nov 20.

Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, United States.

The mucosal inflammatory response to (Gc) is characterized by recruitment of neutrophils to the site of infection. Gc survives exposure to neutrophils by limiting the ability of neutrophils to make antimicrobial products and by expressing factors that defend against these products. The multiple transferable resistance (Mtr) system is a tripartite efflux pump, comprised of the inner membrane MtrD, the periplasmic attachment protein MtrC, and the outer membrane channel MtrE. Read More

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November 2018

Antimicrobial resistance prediction and phylogenetic analysis of Neisseria gonorrhoeae isolates using the Oxford Nanopore MinION sequencer.

Sci Rep 2018 Dec 4;8(1):17596. Epub 2018 Dec 4.

WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections, Department of Laboratory Medicine, Clinical Microbiology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.

Antimicrobial resistance (AMR) in Neisseria gonorrhoeae is common, compromising gonorrhoea treatment internationally. Rapid characterisation of AMR strains could ensure appropriate and personalised treatment, and support identification and investigation of gonorrhoea outbreaks in nearly real-time. Whole-genome sequencing is ideal for investigation of emergence and dissemination of AMR determinants, predicting AMR, in the gonococcal population and spread of AMR strains in the human population. Read More

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December 2018

Gonorrhea and syphilis co-infection and related risk factors in HIV patients from Shiraz, South of Iran.

Caspian J Intern Med 2018 ;9(4):397-402

Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Background: and as an obligate human pathogen are two common causes of sexually transmitted diseases (STDs). The present study aimed to determine the prevalence of and among human immunodeficiency virus (HIV) patients in the southwest Iran.

Methods: This retrospective study was performed from 2004 to 2013, on HIV patients who were tested for detection of gonorrhea and syphilis infection at Shiraz HIV/AIDS Research Center. Read More

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January 2018
1 Read

Genome-wide discovery of epistatic loci affecting antibiotic resistance in Neisseria gonorrhoeae using evolutionary couplings.

Nat Microbiol 2018 Dec 3. Epub 2018 Dec 3.

Department of Systems Biology, Harvard Medical School, Boston, MA, USA.

Genome analysis should allow the discovery of interdependent loci that together cause antibiotic resistance. In practice, however, the vast number of possible epistatic interactions erodes statistical power. Here, we extend an approach that has been successfully used to identify epistatic residues in proteins to infer genomic loci that are strongly coupled. Read More

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December 2018
1 Read

The role of gonococcal Neisserial surface protein A (NspA) in serum resistance and comparison of its factor H binding properties with that of its meningococcal counterpart.

Infect Immun 2018 Dec 3. Epub 2018 Dec 3.

Department of Medicine, Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, MA 01605.

the causative agent of gonorrhea, has evolved several mechanisms to subvert complement, including binding of the complement inhibitor factor H (FH). We previously reported FH binding to independently of lipooligosaccharide (LOS) sialylation. Here we report that Factor H-like protein 1 (FHL-1), which contains FH domains 1 through 7 and possesses complement inhibitory activity, also binds to The ligand for both FH and FHL-1 was identified as Neisserial surface protein A (NspA), which has previously been identified as a ligand for these molecules on As with -NspA, NspA bound FH/FHL-1 through FH domains 6 and 7. Read More

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December 2018

PBP3 and PBP4 facilitate NOD1 agonist peptidoglycan fragment release and survival in stationary phase.

Infect Immun 2018 Dec 3. Epub 2018 Dec 3.

Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, Wisconsin, USA

releases peptidoglycan fragments during growth, and these molecules induce an inflammatory response in the human host. The proinflammatory molecules include peptidoglycan monomers, peptidoglycan dimers, and free peptides. These molecules can be released by the actions of lytic transglycosylases or an amidase. Read More

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December 2018

Stably high azithromycin resistance and decreasing ceftriaxone susceptibility in Neisseria gonorrhoeae in 25 European countries, 2016.

BMC Infect Dis 2018 Dec 3;18(1):609. Epub 2018 Dec 3.

WHO Collaborating Centre for Gonorrhea and other STIs, Örebro University, Örebro, Sweden.

Background: The European Gonococcal Antimicrobial Surveillance Programme (Euro-GASP) performs annual sentinel surveillance of Neisseria gonorrhoeae susceptibility to therapeutically relevant antimicrobials across the European Union/European Economic Area (EU/EEA). We present the Euro-GASP results from 2016 (25 countries), linked to patient epidemiological data, and compared with data from previous years.

Methods: Agar dilution and minimum inhibitory concentration (MIC) gradient strip methodologies were used to determine the antimicrobial susceptibility (using EUCAST breakpoints) of 2660 N. Read More

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December 2018

Bacterial Vaginosis: An insight into the prevalence, alternative regimen treatments and it's associated resistance patterns.

Microb Pathog 2018 Nov 28;127:21-30. Epub 2018 Nov 28.

Atta-Ur-Rahman School of Applied Bio-Sciences, Department of Healthcare Biotechnology, National University of Sciences and Technology, Islamabad, 44000, Pakistan. Electronic address:

Bacterial Vaginosis (BV) is a complex polymicrobial infection of vagina that shifts the paradigms of vaginal flora from lactobacilli to opportunistic pathogens. BV is catagorized by greyish white discharge, pH greater than 4.5. Read More

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November 2018
5 Reads

Adverse pregnancy and neonatal outcomes associated with a systematic review and meta-analysis protocol.

BMJ Open 2018 Nov 28;8(11):e024175. Epub 2018 Nov 28.

Public Health Interventions Research Group, Kirby Institute, University of New South Wales, Sydney, New South Wales, Australia.

Introduction: Several bacterial sexually transmitted and genital mycoplasma infections during pregnancy have been associated with poor pregnancy and perinatal outcomes. Comprehensive and systematic information about associations between sexually transmitted infections (STI) and genital infections in pregnancy and adverse perinatal outcomes is needed to improve understanding about the evidence for causal associations between these infections and adverse pregnancy and neonatal outcomes. Our primary objective is to systematically review the literature about associations between: (1) in pregnancy and preterm birth; (2) in pregnancy and preterm birth; (3) and/or in pregnancy and preterm birth. Read More

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November 2018
1 Read

Neonatal conjunctivitis in the New Zealand Midland region.

N Z Med J 2018 Nov 30;131(1486):9-17. Epub 2018 Nov 30.

Ophthalmologist, Clinical Senior Lecturer, Ophthalmology Department, Department of Medicine, Hauora Tairawhiti, University of Otago, Gisborne.

Aims: To assess the incidence of chlamydial and gonorrhoeal neonatal conjunctivitis (CON and GON), across six district health boards in the greater Midland region of New Zealand.

Methods: All positive nucleic acid amplification test (NAAT) eye swabs for Chlamydia trachomatis and NAAT and bacterial eye swabs for Neisseria gonorrhoeae in infants under one year of age were retrieved from three laboratories from 2013-2016. Incidence density rates were calculated using births information from Statistics New Zealand. Read More

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November 2018
3 Reads

Things Are Not Always What They Seem: Two Cases of Child Maltreatment Presenting With Common Pediatric Chief Complaints.

Pediatr Emerg Care 2018 Nov 26. Epub 2018 Nov 26.

Hospital Medicine, Department of Pediatrics, and.

We describe 2 cases of child maltreatment who presented as common pediatric conditions: preseptal cellulitis and gastroenteritis. The first case is an 8-year-old girl who presented with progressive right eye pain, swelling, and discharge. She was initially treated for preseptal cellulitis, but eye cultures ultimately grew Neisseria gonorrhoeae. Read More

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November 2018

Molecular diagnosis of genital tract infections among HIV-positive women in Iran.

Iran J Microbiol 2018 Aug;10(4):233-241

Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

Background And Objectives: Human immunodeficiency virus (HIV)-infected women are usually at a higher risk of sexually transmitted infections (STIs) than others. The objective of this study was to characterize the prevalence of human papilloma virus (HPV), herpes simplex virus (HSV), (CT), and (NG), and associated risk factors among HIV-infected women in Fars province, Iran.

Materials And Methods: In this cross-sectional study, cervical swab samples were collected from 71 HIV-infected women, aged 17-45 years (mean ± standard deviation: 31. Read More

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Mechanistic Basis for Decreased Antimicrobial Susceptibility in a Clinical Isolate of Neisseria gonorrhoeae Possessing a Mosaic-Like Efflux Pump Locus.

MBio 2018 Nov 27;9(6). Epub 2018 Nov 27.

Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia, USA

Recent reports suggest that mosaic-like sequences within the (ultiple ransferable esistance) efflux pump locus of , likely originating from commensal sp. by transformation, can increase the ability of gonococci to resist structurally diverse antimicrobials. Thus, acquisition of numerous nucleotide changes within the gene encoding the transcriptional repressor (MtrR) of the efflux pump-encoding operon or overlapping promoter region for both along with those that cause amino acid changes in the MtrD transporter protein were recently reported to decrease gonococcal susceptibility to numerous antimicrobials, including azithromycin (Azi) (C. Read More

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November 2018

Multidrug-resistant isolate, belonging to the internationally spreading Japanese FC428 clone, with ceftriaxone resistance and intermediate resistance to azithromycin, Ireland, August 2018.

Euro Surveill 2018 Nov;23(47)

Authors contributed equally to the work and share joint authorship.

We describe a multidrug-resistant urethritis case with ceftriaxone resistance and azithromycin intermediate resistance in a heterosexual man in Ireland, August 2018. Whole-genome sequencing showed that the isolate IR72 belongs to the internationally spreading multidrug-resistant ceftriaxone-resistant FC428 clade, initially described in Japan in 2015. IR72 was assigned MSLT ST1903, NG-MAST ST17842 and NG-STAR type 1133, including the ceftriaxone resistance-mediating 60. Read More

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November 2018

The prevalence of bacteriospermia in infertile men and association with semen quality in Southwestern Iran.

Infect Disord Drug Targets 2018 Nov 23. Epub 2018 Nov 23.

Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz. Iran.

Introduction: Infertility considered as a social and public health issue and estimated that the most of these infertile couple are residents of developing countries. Infectious diseases including the history of sexually transmitted infections (STIs) may impact on male reproductive function. Therefore, the present study aimed to investigate the prevalence of bacterial contaminants of semen and probable association with sperm quality of infertile men in Iranian population. Read More

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November 2018
3 Reads

Multiplex TaqMan real-time PCR platform for detection of Neisseria gonorrhoeae with decreased susceptibility to ceftriaxone.

Diagn Microbiol Infect Dis 2018 Oct 31. Epub 2018 Oct 31.

Department of Clinical Laboratory, Tai'an Central Hospital, Tai'an 271000, China. Electronic address:

A multiplex TaqMan real-time PCR platform was developed in this study for combined detection of opa and/or porA genes (identification of N. gonorrhoeae) and the key mutations (Ala501Val/Thr/Pro, and/or Gly545Ser) in penicillin-binding protein 2 (PBP2) associated with decreased susceptibility to extended-spectrum cephalosporins (ESCs). Firstly, the specificities of the TaqMan probes/primers for the multiplex TaqMan real time PCR platform were confirmed by Basic Local Alignment Search Tool (BLAST) analysis. Read More

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October 2018

Caution Is Required When Using Non-Food and Drug Administration-Cleared Assays to Diagnose Sexually Transmitted Infections in Children.

J Pediatr 2018 Nov 19. Epub 2018 Nov 19.

Department of Pediatrics, Division of Infectious Diseases, State University of New York, Downstate Medical Center, Brooklyn, NY.

Nucleic acid amplification testing is the gold-standard for Chlamydia trachomatis and Neisseria gonorrhoeae testing in adults. We present 3 pediatric cases in which testing resulted in probable false-positive results. Clinicians should avoid tests without clearance from a regulatory agency and should maintain consistent communication with laboratories. Read More

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November 2018
1 Read

Culture- and PCR-based detection of BV associated microbiological profile of the removed IUDs and correlation with the time period of IUD in place and the presence of the symptoms of genital tract infection.

Ann Clin Microbiol Antimicrob 2018 Nov 22;17(1):40. Epub 2018 Nov 22.

Institute of Clinical Microbiology, Albert Szent-Györgyi Clinical Center, Faculty of Medicine, University of Szeged, Semmelweis St. 6, Szeged, 6725, Hungary.

Objectives: The long-term use of intrauterine devices (IUDs) may lead to biofilm formation on the surface. The aim of this study was to perform the culture- and PCR-based detection of bacteria/fungi from the biofilm of the removed IUDs with different time periods in place.

Methods: For a 2-year period, 100 IUD users were involved in the study. Read More

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November 2018
4 Reads

In vitro activity of ceftazidime-avibactam, ceftolozane-tazobactam, and other comparable agents against clinically important Gram-negative bacilli: results from the 2017 Surveillance of Multicenter Antimicrobial Resistance in Taiwan (SMART).

Infect Drug Resist 2018 26;11:1983-1992. Epub 2018 Oct 26.

Department of Laboratory Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan.

Objectives: We investigated the in vitro antimicrobial susceptibilities of clinically important Gram-negative bacteria (GNB) from 16 major teaching hospitals in Taiwan in 2017.

Materials And Methods: (n=686) and bloodstream isolates (n=673), non-typhoid (NTS; n=221) from various sources, species (n=21) from fecal samples, and (n=129) from the genitourinary tract were collected. Antibiotic minimum inhibitory concentrations (MICs) were determined using the broth microdilution method. Read More

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October 2018
5 Reads

Neonatal Conjunctivitis Caused by Neisseria cinerea: A Case of Mistaken Identity.

J Pediatric Infect Dis Soc 2018 Nov 21. Epub 2018 Nov 21.

Division of Pediatric Infectious Diseases, Department of Pediatrics, NYU Winthrop Hospital, Mineola, New York.

We report a case of a 3-day-old boy with Neisseria cinerea conjunctivitis, originally misidentified as Neisseria gonorrhoeae conjunctivitis. Neonates are at increased risk for disseminated gonococcal infection, and physicians should be cognizant of N cinerea and its potential to be mistaken for N gonorrhoeae. Read More

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November 2018
2 Reads

Establishment of a gonococcal antimicrobial surveillance programme (GASP), in accordance with WHO standards, in Côte d'Ivoire, Western Africa, 2014-2017.

Sex Transm Dis 2018 Nov 19. Epub 2018 Nov 19.

World Health Organization Collaborating Centre for Gonorrhoea and other STIs, Department of Laboratory Medicine, Microbiology, Örebro University Hospital, Örebro, Sweden.

Background: Antimicrobial resistance (AMR) in Neisseria gonorrhoeae is compromising the treatment of gonorrhea globally. Recent AMR data are extremely limited in Africa, and mainly totally lacking in Western Africa including Côte d'Ivoire. This study i) established a quality-assured gonococcal antimicrobial surveillance programme (GASP), according to WHO quality criteria, ii) investigated the AMR to eight therapeutic antimicrobials in gonococcal isolates from 2014 to 2017, and iii) provided evidence for updating the national STD syndromic management guidelines in Côte d'Ivoire. Read More

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November 2018
6 Reads

Meningococcal B vaccine: The journey from research to real world experience.

Expert Rev Vaccines 2018 Nov 20. Epub 2018 Nov 20.

d Vaccine Development Leader , Research and Development Centre. GSK , 14200 Shady Grove Road, Rockville , USA.

Introduction: Neisseria meningitidis serogroup B (MenB) is the most common cause of bacterial meningitis in many industrialized countries and occurs at any age. The highest incidence is in infants aged <1 year, followed by children and adolescents. Four-component MenB vaccine (4CMenB, Bexsero) is the only MenB vaccine authorized for use in all age-groups. Read More

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November 2018
2 Reads

WHO laboratory validation of Xpert CT/NG and Xpert TV on the GeneXpert system verifies high performances.

APMIS 2018 Dec;126(12):907-912

WHO Collaborating Centre for Gonorrhoea and Other Sexually Transmitted Infections, National Reference Laboratory for Sexually Transmitted Infections, Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.

Effective tests for diagnosis of sexually transmitted infections (STIs), used point of care to inform treatment and management decisions, are urgently needed. We evaluated the analytical sensitivity and specificity of the Xpert CT/NG and Xpert TV tests, examining 339 samples spiked with phenotypically and/or genetically diverse strains of Neisseria gonorrhoeae, Chlamydia trachomatis, and Trichomonas vaginalis, and other related species that may cross-react. The APTIMA Combo 2 test and APTIMA TV test were used as reference tests. Read More

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December 2018
2 Reads

Association between intensity of STI screening and development of antimicrobial resistance in in 12 cities in the USA: An ecological study.

Authors:
Chris R Kenyon

F1000Res 2018 10;7:1237. Epub 2018 Aug 10.

Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, 2000, Belgium.

In this study, we assessed if there was a city-level association between sexually transmitted infection (STI) screening intensity in men who have sex with men and antimicrobial sensitivity in in the United States, 2007 to 2013.  We found positive associations between STI screening intensity and increases in minimum inhibitory concentrations for cefixime and azithromycin, but not ceftriaxone. Read More

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August 2018
1 Read

Antimicrobial resistance and molecular characterization of Neisseria gonorrhoeae isolates in Fukuoka, Japan, from 1996 to 2016.

J Glob Antimicrob Resist 2018 Nov 15. Epub 2018 Nov 15.

Department of Infection Control and Prevention, Faculty of Nursing, Toho University, Tokyo, Japan.

Objectives: We examined the antimicrobial resistance and molecular characteristics of Neisseria gonorrhoeae isolates obtained from 1996 -2005 (n=200) and 2008-2016 (n=200) in Fukuoka, Japan.

Methods: MICs were determined by agar dilution and sequence types (STs) were examined using N. gonorrhoeae multi-antigen sequence typing (NG-MAST). Read More

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November 2018
3 Reads

The demographic and clinical profiles of women presenting with vaginal discharge syndrome at primary care facilities in South Africa: Associations with age and implications for management.

S Afr Med J 2018 Oct 2;108(10):876-880. Epub 2018 Oct 2.

Centre for HIV and STIs, National Institute for Communicable Diseases, Johannesburg, South Africa; School of Public Health, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.

Background: Current South African guidelines for the management of vaginal discharge syndrome (VDS) do not recommend treatment for sexually transmitted infection (STI) pathogens for women aged ≥35 years whose partners do not have male urethritis syndrome. The guideline assumes that older women are unlikely to have an STI and that their partners do not have asymptomatic infections.

Objectives: To describe the demographic, behavioural and clinical characteristics of women with VDS, comparing older women (≥35 years) with younger women, and to determine the performance of age alone as a criterion for predicting the presence of STI. Read More

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October 2018
1 Read

Disseminated gonococcal infections in patients receiving eculizumab: a case series.

Clin Infect Dis 2018 Nov 12. Epub 2018 Nov 12.

Division of Pharmacovigilance, Office of Surveillance and Epidemiology, Center for Drug Evaluation and Research, US Food and Drug Administration, Silver Spring, MD, USA.

Background: Gonorrhea is the second most commonly reported notifiable condition in the United States (U.S.). Read More

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November 2018
7 Reads

Take three, test one: a cross-sectional study to evaluate the molecular detection of Chlamydia trachomatis and Neisseria gonorrhoeae in pooled pharyngeal, anorectal and urine samples versus single-site testing among men who have sex with men in Belgium.

Acta Clin Belg 2018 Nov 12:1-5. Epub 2018 Nov 12.

a HIV/STI Reference Laboratory, Department of Clinical Sciences , Institute of Tropical Medicine , Antwerp , Belgium.

Objectives: To investigate the efficacy of performing a pooling strategy of triple-anatomical site samples (pharyngeal, anorectal and urine samples) for simultaneous Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) nucleic acid amplification detection.

Methods: A total of 117 specimen sets (pharyngeal, anorectal and urine) were collected from 98 men between 2014 and 2016. Double sampling of pharyngeal, anorectal and urine samples allowed for pooled and unpooled analyses using a multiplex Abbott Real Time CT/NG assay, together with confirmatory PCR testing in case of CT/NG positivity. Read More

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November 2018

Antimicrobial resistance and molecular characteristics of Neisseria gonorrhoeae isolates from MSM.

Int J Infect Dis 2018 Nov 6. Epub 2018 Nov 6.

Global Health and Tropical Medicine (GHTM) Tropical Clinic/Institute of Hygiene and Tropical Medicine, New University of Lisbon (UNL), Lisbon, Portugal.

Objectives: To analyze the susceptibility of Neisseria gonorrhoeae isolates to penicillin (Pen), cefixime (Cfm), ceftriaxone (Cro), tetracycline (Tet), ciprofloxacin (Cip), azithromycin (Azm) and spectinomycin (Spt). To verify the presence of mutations in resistant genes.

Methods: Antibiotic susceptibility testing was carried out by Etest method in 30N. Read More

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November 2018

The first year of the global Enhanced Gonococcal Antimicrobial Surveillance Programme (EGASP) in Bangkok, Thailand, 2015-2016.

PLoS One 2018 9;13(11):e0206419. Epub 2018 Nov 9.

Thailand Ministry of Public Health-U.S. Centers for Disease Control and Prevention Collaboration, Nonthaburi, Thailand.

Antimicrobial-resistant Neisseria gonorrhoeae (NG) infection is a global public health threat, and there is a critical need to monitor patterns of resistance and risk factors. In collaboration with the World Health Organization (WHO), the U.S. Read More

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November 2018
2 Reads

Pili mediated intercellular forces shape heterogeneous bacterial microcolonies prior to multicellular differentiation.

Sci Rep 2018 Nov 8;8(1):16567. Epub 2018 Nov 8.

Brooklyn College of CUNY, Department of Biology, Brooklyn, USA.

Microcolonies are aggregates of a few dozen to a few thousand cells exhibited by many bacteria. The formation of microcolonies is a crucial step towards the formation of more mature bacterial communities known as biofilms, but also marks a significant change in bacterial physiology. Within a microcolony, bacteria forgo a single cell lifestyle for a communal lifestyle hallmarked by high cell density and physical interactions between cells potentially altering their behaviour. Read More

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November 2018
6 Reads

Single-Dose Zoliflodacin (ETX0914) for Treatment of Urogenital Gonorrhea.

N Engl J Med 2018 11;379(19):1835-1845

From the Louisiana State University Health Sciences Center, New Orleans (S.N.T.); University of Alabama at Birmingham, Birmingham (J. Marrazzo, E.W.H.); University of Washington, Seattle (J. Marrazzo); Indiana University, Indianapolis (B.E.B.); University of North Carolina, Chapel Hill (A.C.S.), and FHI 360, Durham (S.M.J.) - both in North Carolina; Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Disease, Bethesda (J.L.), and Emmes, Rockville (M.R.W., H.K.) - both in Maryland; and Entasis Therapeutics, Waltham, MA (K.L., J. Mueller).

Background: Antibiotic-resistant Neisseria gonorrhoeae has prompted the development of new therapies. Zoliflodacin is a new antibiotic that inhibits DNA biosynthesis. In this multicenter, phase 2 trial, zoliflodacin was evaluated for the treatment of uncomplicated gonorrhea. Read More

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November 2018
20 Reads

Neisseria gonorrhoeae - Rising Infection Rates, Dwindling Treatment Options.

N Engl J Med 2018 Nov;379(19):1795-1797

From the Division of Disease Control, New York City Department of Health and Mental Hygiene, New York (S.B., D.C.D.); and the Division of STD Prevention, National Center for HIV, Viral Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta (S.B.).

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November 2018
6 Reads

Urogenital pathogens, associated with Trichomonas vaginalis, among pregnant women in Kilifi, Kenya: a nested case-control study.

BMC Infect Dis 2018 Nov 6;18(1):549. Epub 2018 Nov 6.

Laboratory Bacteriology Research, Faculty of Medicine and Health Sciences, Ghent University, De Pintelaan, 185, Ghent, Belgium.

Background: Screening of curable sexually transmitted infections is frequently oriented towards the diagnosis of chlamydia, gonorrhea, syphilis and trichomoniasis, whereas other pathogens, sometimes associated with similar urogenital syndromes, remain undiagnosed and/or untreated. Some of these pathogens are associated with adverse pregnancy outcomes.

Methods: In a nested case-control study, vaginal swabs from 79 pregnant women, i. Read More

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November 2018
9 Reads

Home Self-Collection by Mail to Test for Human Papillomavirus and Sexually Transmitted Infections.

Obstet Gynecol 2018 Dec;132(6):1412-1420

Departments of Epidemiology and Health Behavior, Gillings School of Global Public Health, the School of Medicine, and the Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina; Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China; the Department of Biological and Biomedical Sciences, College of Arts and Science, North Carolina Central University, Durham, North Carolina; and the American Sexual Health Association, Durham, North Carolina.

Objective: To evaluate the validity and acceptability of at-home self-collection to test for high-risk human papillomavirus (HPV) and sexually transmitted infections among women overdue for cervical cancer screening by national guidelines.

Methods: Low-income, infrequently screened women were recruited from the general population in North Carolina to participate in an observational study. Participants provided two self-collected cervicovaginal samples (one at home and one in the clinic) and a clinician-collected cervical sample. Read More

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December 2018
4 Reads

Whole-Genome Sequencing of Russian Neisseria Gonorrhoeae Isolates Related to ST 1407 Genogroup.

Acta Naturae 2018 Jul-Sep;10(3):68-76

State Research Centre of Dermatovenerology and Cosmetology, Korolenko Str., 3/6, Moscow, 107076 , Russia.

The whole-genome sequencing data of three strains isolated in the Russian Federation in 2015 are presented. According to the NG-MAST protocol, these strains are related to the globally spread ST 1407 genogroup. The analysis of their resistomes showed the absence of genes and the presence of wild-type alleles of and genes, and these patterns explain the susceptibility of the sequenced strains to aminocyclitols (spectinomycin) and macrolides (azithromycin). Read More

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November 2018

Acceptability and efficacy of partner notification for curable sexually transmitted infections in sub-Saharan Africa: A systematic review.

Int J STD AIDS 2018 Nov 5:956462418803983. Epub 2018 Nov 5.

1 Department of Infectious Disease, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.

Comprehensive case management of sexually transmitted infections (STIs) includes partner notification. We reviewed the recent literature evaluating the acceptability and efficacy of partner notification strategies (i.e. Read More

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November 2018
2 Reads

Use of a Rapid Diagnostic for Chlamydia trachomatis and Neisseria gonorrhoeae for Women in the Emergency Department Can Improve Clinical Management: Report of a Randomized Clinical Trial.

Ann Emerg Med 2018 Nov 1. Epub 2018 Nov 1.

Department of Emergency Medicine, Johns Hopkins University, Baltimore, MD.

Study Objective: In emergency departments (EDs), diagnosis and treatment of Chlamydia trachomatis and Neisseria gonorrhoeae are challenging. We conducted a randomized clinical trial to assess rapid C trachomatis and N gonorrhoeae testing on overtreatment and undertreatment of women evaluated for C trachomatis and N gonorrhoeae.

Methods: Women undergoing pelvic examinations and C trachomatis and N gonorrhoeae testing (n=254) were randomized to control or rapid test groups. Read More

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November 2018

Complete ciprofloxacin resistance in gonococcal isolates in an urban Ugandan clinic: findings from a cross-sectional study.

Int J STD AIDS 2018 Nov 4:956462418799017. Epub 2018 Nov 4.

3 Infectious Disease Institute, Makerere University College of Health Sciences, Kampala, Uganda.

Antimicrobial resistance (AMR) to gonorrhoea is a threat to global health security. There have been concerns expressed that countries with high rates of disease have poor surveillance. The objectives of the study were to determine the AMR patterns of Neisseria gonorrhoeae clinical isolates to antimicrobial agents in patients with HIV or high risk of HIV acquisition, to compare the concordance of disk diffusion and agar dilution as methods for determining AMR to N. Read More

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November 2018
1 Read

Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real-time PCR Kits in the detection of six sexually transmitted disease pathogens.

J Clin Lab Anal 2018 Nov 2:e22703. Epub 2018 Nov 2.

Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.

Background: The DiaPlexQ™ STI6 Detection Kit (DiaPlexQ; Solgent Co., Ltd., Daejeon, South Korea) is a multiplex real-time PCR assay for the detection of the following sexually transmitted disease (STD) pathogens: Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis, Trichomonas vaginalis, Ureaplasma urealyticum, and Mycoplasma genitalium. Read More

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November 2018
5 Reads

[Current diagnosis and treatment of sexually transmitted infections].

Urologe A 2018 Dec;57(12):1436-1444

Klinik für Urologie, Kinderurologie und Andrologie, Justus-Liebig-Universität Gießen, Rudolf-Buchheim-Str. 7, 35392, Gießen, Deutschland.

Background: In recent years, there has been an increasing incidence of sexually transmitted infections (STI), which in turn play a major role in urological clinical routine.

Objectives: Which STIs are relevant in urological clinical routine? What kind of recommendations for diagnosis and treatment exist?

Materials And Methods: Review of evidence-based recommendations from the literature, current guidelines of the European Association of Urology and the Robert-Koch-Institute.

Results: STIs can be differentiated into those that cause genital, anal, perianal or oral ulcers from STIs that cause urethritis, cervicitis, or urethral or vaginal discharge. Read More

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December 2018
1 Read

Neisseria gonorrhoeae 23S rRNA A2059G mutation is the only determinant necessary for high-level azithromycin resistance and improves in vivo biological fitness.

J Antimicrob Chemother 2018 Oct 29. Epub 2018 Oct 29.

Department of Microbiology and Parasitology, School of Medicine, Zhejiang University, Hangzhou, China.

Objectives: The global emergence of Neisseria gonorrhoeae isolates displaying high-level azithromycin resistance is a major concern for the currently recommended azithromycin/ceftriaxone dual therapy. N. gonorrhoeae high-level azithromycin resistance has been associated with an A2059G mutation in 23S rRNA. Read More

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October 2018
5 Reads

Does gonorrhoea screening intensity play a role in the early selection of antimicrobial resistance in men who have sex with men (MSM)? A comparative study of Belgium and the United Kingdom.

F1000Res 2018 10;7:569. Epub 2018 May 10.

HIV/STI Reference Laboratory, Institute of Tropical Medicine, Antwerp, Belgium.

It is unclear why antimicrobial resistance in in the United Kingdom (UK) and the United States has tended to first appear in men who have sex with men (MSM). We hypothesize that increased exposure to antimicrobials from intensive STI screening programmes plays a role. We assess if there is a difference in the distribution of azithromycin, cefixime and ceftriaxone minimum inhibitory concentrations (MICs) between MSM and women in the United Kingdom (UK) where 70% of MSM report STI screening in the past year vs. Read More

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May 2018
1 Read

Prevalence, risk factors, and medical costs of Chlamydia trachomatis infections in Shandong Province, China: a population-based, cross-sectional study.

BMC Infect Dis 2018 Oct 26;18(1):534. Epub 2018 Oct 26.

Shandong Provincial Institute of Dermatology and Venereology, Shandong Academy of Medical Sciences, 27397 Jingshi Road, Jinan, 250022, China.

Background: A population-based study of Chlamydia trachomatis (CT) infections is essential in designing a specific control program; however, no large investigation of CT infections among the general population in mainland China has been conducted since 2000. We aimed to determine the prevalence, risk factors, and associated medical costs of CT among residents, 18-49 years of age, in Shandong, China.

Methods: From May to August 2016, a multistage probability sampling survey involving 8074 individuals was distributed. Read More

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October 2018
3 Reads
2.610 Impact Factor

PROVISION OF STI SERVICES IN A MOBILE CLINIC REVEALS HIGH UNMET NEED IN REMOTE AREAS OF SOUTH AFRICA: A CROSS-SECTIONAL STUDY.

Sex Transm Dis 2018 Oct 22. Epub 2018 Oct 22.

Anova Health Institute, Johannesburg, South Africa.

Background: The burden of sexually transmitted infections (STIs) in areas of sub-Saharan Africa with poor access to healthcare services is not well documented. In remote areas of South Africa we investigated the prevalence of STIs and approaches to providing STI services through a mobile clinic.

Methods: We recruited 251 adult women visiting a mobile clinic that normally provides general health education and screening services, but not STI care. Read More

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October 2018
4 Reads