Auris Nasus Larynx 2021 Jun 17;48(3):487-495. Epub 2020 Oct 17.
Department of Nuclear Medicine, The First People's Hospital of Jingzhou City, The First Affiliated Hospital of Yangtze University, Jingzhou 434000, Hubei Province, China. Electronic address:
Objective: To investigate the sineoculis homeobox homolog 1 (SIX1) affect the epithelial mesenchymal transition (EMT) in papillary thyroid carcinoma (PTC) through regulating TGF-β/Smad2/3 signaling pathway.
Methods: The SIX1 expression in cytological specimens, tissues or PTC cell lines was detected by qRT-PCR, western blotting or immunohistochemistry. A series of vitro experiments including flow cytometry, CCK-8, wound-healing and Transwell were used to evaluate the biological characteristics in a PTC cell line (NPA cells), which were divided into Blank, Negative control (NC), SIX1, SIX1-siRNA, LY-364947 (TGF-β/Smad2/3 pathway inhibitor) and SIX1 + LY-364947 groups. Read More