68,248 results match your criteria Glycogen Storage Diseases Types I-VII


A detailed in silico analysis of the amylolytic family GH126 and its possible relatedness to family GH76.

Carbohydr Res 2020 Jun 23;494:108082. Epub 2020 Jun 23.

Laboratory of Protein Evolution, Institute of Molecular Biology, Slovak Academy of Sciences, SK-84551, Bratislava, Slovakia; Department of Biology, Faculty of Natural Sciences, University of SS. Cyril and Methodius, SK-91701, Trnava, Slovakia. Electronic address:

The glycoside hydrolase (GH) family 126 was established based on the X-ray structure determination of the amylolytic enzyme CPF_2247 from Clostridium perfringens genome. Its original identification as a putative carbohydrate-active enzyme was based on its low, yet significant sequence identity to members of the family GH8, which are inverting endo-β-1,4-glucanases. As the family GH8 forms the clan GH-M with GH48, the CPF_2247 protein also exhibits similarities with members of the family GH48. Read More

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http://dx.doi.org/10.1016/j.carres.2020.108082DOI Listing

Renal injury and hepatic effects from the methylimidazolium ionic liquid M8OI in mouse.

Ecotoxicol Environ Saf 2020 Jul 4;202:110902. Epub 2020 Jul 4.

Health Protection Research Unit, Wolfson Building, Newcastle University, Newcastle Upon Tyne, NE2 4AA, United Kingdom; Institute Translational and Clinical Research, Level 4 Leech, Newcastle University, Newcastle Upon Tyne, NE2 4HH, United Kingdom. Electronic address:

The ionic liquid 1-octyl-3-methylimidazolium (M8OI) has been found in the environment and identified as a hazard for triggering the liver disease primary biliary cholangitis (PBC). Given limited toxicity data for M8OI and other structurally-related ionic liquids, target organs for M8OI toxicity were examined. Adult male C57Bl6 mice were acutely exposed to 0-10 mg/kg body weight M8OI via 2 intraperitoneal injections (time zero and 18 h) and effects examined at 24 h. Read More

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http://dx.doi.org/10.1016/j.ecoenv.2020.110902DOI Listing

Modulation of LPS-induced inflammatory cytokine production by a novel glycogen synthase kinase-3 inhibitor.

Eur J Pharmacol 2020 Jul 4:173340. Epub 2020 Jul 4.

Department of Chemical and Biomolecular Engineering, Ohio University, Athens, OH, 45701, USA; Biomedical Engineering Program, Ohio University, Athens, OH, 45701, USA. Electronic address:

Sepsis is a serious condition that can lead to long-term organ damage and death. At the molecular level, the hallmark of sepsis is the elevated expression of a multitude of potent cytokines, i.e. Read More

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http://dx.doi.org/10.1016/j.ejphar.2020.173340DOI Listing

Skeletal muscle dysregulation in rheumatoid arthritis: Metabolic and molecular markers in a rodent model and patients.

PLoS One 2020 7;15(7):e0235702. Epub 2020 Jul 7.

Division of Physiology, Pharmacology and Neuroscience, School of Life Science, University of Nottingham, Nottingham, England, United Kingdom.

Rheumatoid arthritis (RA) is accompanied by pain, inflammation and muscle weakness. Skeletal muscle inflammation and inactivity are independently associated with muscle insulin resistance and atrophy. Our objective was to identify early molecular and biochemical markers in muscle from a rodent model of RA relative to control and subsequently identify commonality in muscle gene expression between this model and muscle from RA patients. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0235702PLOS

MiR-129-2 weakens proliferation and promotes apoptosis of liver cancer cells by suppressing the Wnt signaling pathway.

Eur Rev Med Pharmacol Sci 2020 Jun;24(12):6665-6673

Department of Interventional Oncology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Objective: To explore the effects of micro ribonucleic acid-129-2 (miR-129-2) on proliferation and migration of liver cancer cells and its possible mechanism.

Patients And Methods: The expression level of miR-129-2 was measured in liver cancer tissues and adjacent tissues from patients with liver cancer. Its level in liver cancer HepG2 cells and normal liver cells L-02 was also detected via quantitative polymerase chain reaction (qPCR). Read More

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http://dx.doi.org/10.26355/eurrev_202006_21653DOI Listing

A negative reciprocal regulatory axis between cyclin D1 and HNF4α modulates cell cycle progression and metabolism in the liver.

Proc Natl Acad Sci U S A 2020 Jul 6. Epub 2020 Jul 6.

Gastroenterology Division, Minneapolis VA Health Care System, Minneapolis, MN 55417;

Hepatocyte nuclear factor 4α (HNF4α) is a master regulator of liver function and a tumor suppressor in hepatocellular carcinoma (HCC). In this study, we explore the reciprocal negative regulation of HNF4α and cyclin D1, a key cell cycle protein in the liver. Transcriptomic analysis of cultured hepatocyte and HCC cells found that cyclin D1 knockdown induced the expression of a large network of HNF4α-regulated genes. Read More

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http://dx.doi.org/10.1073/pnas.2002898117DOI Listing

KLF4 Acts as a wt-CFTR Suppressor through an AKT-Mediated Pathway.

Cells 2020 Jul 2;9(7). Epub 2020 Jul 2.

BioISI-Biosystems & Integrative Sciences Institute, Faculty of Sciences, University of Lisbon, 1749-016 Lisbon, Portugal.

Cystic Fibrosis (CF) is caused by >2000 mutations in the CF transmembrane conductance regulator (CFTR) gene, but one mutation-F508del-occurs in ~80% of patients worldwide. Besides its main function as an anion channel, the CFTR protein has been implicated in epithelial differentiation, tissue regeneration, and, when dysfunctional, cancer. However, the mechanisms that regulate such relationships are not fully elucidated. Read More

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http://dx.doi.org/10.3390/cells9071607DOI Listing

Fibrillar Collagen Type I Participates in the Survival and Aggregation of Primary Hepatocytes Cultured on Soft Hydrogels.

Biomimetics (Basel) 2020 Jun 25;5(2). Epub 2020 Jun 25.

Laboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia (LaNSBioDyT), Facultad de Ciencias, Universidad Nacional Autónoma de Mexico, Ciudad de México CP 04510, Mexico.

Liver is an essential organ that carries out multiple functions such as glycogen storage, the synthesis of plasma proteins, and the detoxification of xenobiotics. Hepatocytes are the parenchyma that sustain almost all the functions supported by this organ. Hepatocytes and non-parenchymal cells respond to the mechanical alterations that occur in the extracellular matrix (ECM) caused by organogenesis and regenerating processes. Read More

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http://dx.doi.org/10.3390/biomimetics5020030DOI Listing

The Presence of Seminal Plasma during Liquid Storage of Pig Spermatozoa at 17 °C Modulates Their Ability to Elicit In Vitro Capacitation and Trigger Acrosomal Exocytosis.

Int J Mol Sci 2020 Jun 25;21(12). Epub 2020 Jun 25.

Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, ES-17003 Girona, Spain.

Although seminal plasma is essential to maintain sperm integrity and function, it is diluted/removed prior to liquid storage and cryopreservation in most mammalian species. This study sought to evaluate, using the pig as a model, whether storing semen in the presence of seminal plasma affects the sperm ability to elicit in vitro capacitation and acrosomal exocytosis. Upon collection, seminal plasma was separated from sperm samples, which were diluted in a commercial extender, added with seminal plasma (15% or 30%), and stored at 17 °C for 48 or 72 h. Read More

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http://dx.doi.org/10.3390/ijms21124520DOI Listing

Insulin Resistance Does Not Impair Mechanical Overload-Stimulated Glucose Uptake, but Does Alter the Metabolic Fate of Glucose in Mouse Muscle.

Int J Mol Sci 2020 Jul 1;21(13). Epub 2020 Jul 1.

Department of Kinesiology, East Carolina University, Greenville, NC 27858, USA.

Skeletal muscle glucose uptake and glucose metabolism are impaired in insulin resistance. Mechanical overload stimulates glucose uptake into insulin-resistant muscle; yet the mechanisms underlying this beneficial effect remain poorly understood. This study examined whether a differential partitioning of glucose metabolism is part of the mechanosensitive mechanism underlying overload-stimulated glucose uptake in insulin-resistant muscle. Read More

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http://dx.doi.org/10.3390/ijms21134715DOI Listing

Tumorigenic Potential of Granulocyte Colony-Stimulating Factor Therapy-A Case Report and Review of Literature.

J Oral Maxillofac Surg 2020 Jun 10. Epub 2020 Jun 10.

Assistant Professor of Surgery and Residency Program Director, Department of Oral and Maxillofacial Surgery, Parkland/UT Southwestern, Dallas, TX.

An association between granulocyte colony-stimulating factor therapy (G-CSFT) in patients with glycogen storage disease type Ib (GSDIb) and the development of giant cell lesions of the maxillofacial complex has emerged. We have reported, to the best of our knowledge, the fourth case of giant cell granuloma (GCG) in a patient with GSDIb undergoing G-CSFT. GSDIb can present with hypoglycemia, hypertriglyceridemia, and neutropenia. Read More

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http://dx.doi.org/10.1016/j.joms.2020.06.006DOI Listing

Clash of the cascades: Release the inhibition.

J Physiol 2020 Jul 6. Epub 2020 Jul 6.

Exercise Metabolism Research Group, Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada.

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http://dx.doi.org/10.1113/JP280253DOI Listing

Nimbolide prevents myocardial damage by regulating cardiac biomarkers, antioxidant level, and apoptosis signaling against doxorubicin-induced cardiotoxicity in rats.

J Biochem Mol Toxicol 2020 Jul 6:e22543. Epub 2020 Jul 6.

ICU, Affiliated Hospital of Weifang Medical University, Weifang, Shandong, China.

The current work planned to assess the protecting properties of nimbolide against doxorubicin (DOX)-treated myocardial damage. Myocardial damage was produced with 2.5 mg/kg of DOX given on alternative days (14 days). Read More

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http://dx.doi.org/10.1002/jbt.22543DOI Listing

Starch in aquafeeds: The benefits of a high amylose to amylopectin ratio and resistant starch content in diets for the carnivorous fish, largemouth bass ().

Br J Nutr 2020 Jun 22:1-29. Epub 2020 Jun 22.

Research Centre of the Agriculture Ministry on Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai, 20136, China.

Aquafeeds for carnivorous species face a nutritional-technological conundrum: containing sufficient starch to meet specific manufacturing requirements for binding, extrusion, and expansion, but ideally containing as little starch as possible owing to their limited ability to utilise carbohydrates. The present study evaluated the effects of dietary starch with different amylose to amylopectin ratios and resistant starch contents on growth performance, hepatic glycogen accumulation, and glucose metabolism of an important cultured carnivorous finfish, largemouth bass (Micropterus salmoides). A common starch source (α-cassava starch) was tested as is or after being enzymatically de-branched at three different inclusion levels in diets for largemouth bass. Read More

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http://dx.doi.org/10.1017/S0007114520002214DOI Listing

GSK-3β inhibition by curcumin mitigates amyloidogenesis via TFEB activation and anti-oxidative Activity in human neuroblastoma cells.

Free Radic Res 2020 Jul 6:1-39. Epub 2020 Jul 6.

Department of Biological Sciences, University of Ulsan, Ulsan, 44610, Republic of Korea.

The translocation of transcription factor EB (TFEB) to the nucleus plays a pivotal role in the regulation of basic cellular processes, such as lysosome biogenesis and autophagy. Autophagy is an intracellular degradation system that delivers cytoplasmic constituents to the lysosome, which is important in maintaining cellular homeostasis during environmental stress. Furthermore, oxidative stress is a critical cause for the progression of neurodegenerative diseases. Read More

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http://dx.doi.org/10.1080/10715762.2020.1791843DOI Listing

Genetic analysis and long-term treatment monitoring of 11 children with glycogen storage disease type IIIa.

J Pediatr Endocrinol Metab 2020 Jul 5. Epub 2020 Jul 5.

Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030,China.

Objectives To investigate the clinical and genetic characteristics of children with glycogen storage disease type IIIa (GSD IIIa) and to explore the muscle involvement and manifestations of GSD IIIa patients. Methods The clinical data of 11 patients with GSD IIIa diagnosed by genetic testing from 2003 to 2019 were retrospectively analyzed. Results Twenty variants of AGL gene were detected in 11 patients, eight of which were novel variants. Read More

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http://dx.doi.org/10.1515/jpem-2019-0453DOI Listing

Circulating exosomal microRNA as potential biomarkers of hepatic injury and inflammation inGlycogen storage disease type 1a.

Dis Model Mech 2020 Jul 3. Epub 2020 Jul 3.

Laboratory of MolecularBiology, IRCCS Istituto Giannina Gaslini, Via G. Gaslini 5, 16147 Genova, Italy

Most patients affected by Glycogen storage disease type 1a (GSD-1a), an inherited metabolic disorder caused by mutations in the enzyme glucose-6-phosphatase-alpha (G6Pase-α), develop renal and liver complications, including development of hepatocellular adenoma/carcinoma. The purpose of this study was to identify potential biomarkers of the pathophysiology of the GSD1a affected liver. To this end, we utilized the plasma exosomes of a murine model of GSD-1a, LS-G6pc mice, to uncover microRNA expression modulation associated with the disease. Read More

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http://dx.doi.org/10.1242/dmm.043364DOI Listing

Investigation of spectroscopic and proteomic alterations underlying prostate carcinogenesis.

J Proteomics 2020 Jun 30;226:103888. Epub 2020 Jun 30.

iBiMED - Institute of Biomedicine, Department of Medical Sciences, University of Aveiro, Aveiro, Portugal. Electronic address:

Prostate cancer (PCa) treatment remains challenging, especially in advanced stages, where the lack of sensitivity and specificity of available biomarkers makes it difficult to establish an accurate prognosis. Therefore, it is imperative to study PCa biology to identify key molecules that can improve PCa management. In this study, eight prostate tumor tissues and paired normal tissues were analyzed using two approaches-Fourier-transform infrared (FT-IR) spectroscopy for spectroscopic profiling of biomolecules and antibody microarray for signaling proteins-with the main goal of identifying metabolic and proteomic changes that enable the distinction between normal and tumor conditions. Read More

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http://dx.doi.org/10.1016/j.jprot.2020.103888DOI Listing

Wnt Activation and Reduced Cell-Cell Contact Synergistically Induce Massive Expansion of Functional Human iPSC-Derived Cardiomyocytes.

Cell Stem Cell 2020 Jul;27(1):50-63.e5

Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address:

Modulating signaling pathways including Wnt and Hippo can induce cardiomyocyte proliferation in vivo. Applying these signaling modulators to human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) in vitro can expand CMs modestly (<5-fold). Here, we demonstrate massive expansion of hiPSC-CMs in vitro (i. Read More

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http://dx.doi.org/10.1016/j.stem.2020.06.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7334437PMC

Screening of thermosensitive autolytic mutant brewer's yeast and transcriptomic analysis of heat stress response.

Can J Microbiol 2020 Jul 3. Epub 2020 Jul 3.

Jiangnan University, 66374, Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Wuxi, Jiangsu, China.

Brewer's yeast has been widely used in the food industry and the autolysates thereof are increasingly being studied for their valuable nutritional compositions. Yeast autolysis is most affected by medium composition and temperature. In this study, a thermosensitive autolytic brewer's yeast P-510 was obtained with ARTP mutagenesis plus 5-Bromo-Chloro-3-Indolyl Phosphate screening. Read More

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http://dx.doi.org/10.1139/cjm-2019-0456DOI Listing

Investigation of the polyphosphate-accumulating organism population in the full-scale simultaneous chemical phosphorus removal system.

Environ Sci Pollut Res Int 2020 Jul 2. Epub 2020 Jul 2.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Department of Environmental Engineering, Beijing University of Technology, No.100 Pingleyuan, Chaoyang District, Beijing, 100124, China.

The simultaneous chemical phosphorus removal (SCPR) process has been widely applied in wastewater treatment plants (WWTPs) due to the high phosphorus removal efficiency through the synergy of biological and chemical phosphorus removal (BPR and CPR). However, phosphorus removal reagents could affect the bacterial community structure in the SCPR system and further affect the BPR process. The BPR phenotypes and community structures in the SCPR system, especially the population of polyphosphate-accumulating organisms (PAOs), are not completely clear. Read More

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http://dx.doi.org/10.1007/s11356-020-09912-9DOI Listing

Case report: adult-onset manifesting heterozygous glycogen storage disease type IV with dilated cardiomyopathy and absent late gadolinium enhancement on cardiac magnetic resonance imaging.

Eur Heart J Case Rep 2020 Jun 3;4(3):1-6. Epub 2020 May 3.

Department of Radiology, Jacobi Medical Center, 1400 Pelham Pkwy S, Bronx, NY 10461, USA.

Background: Glycogen storage disease type IV (GSD IV; Andersen's disease) is a rare autosomal recessive disease caused by mutation in the GBE1 gene. Presentation of GSD IV varies on a continuum of severity and symptomatology ranging from neonatal death to mild adult-onset disease with variable involvement of hepatic, muscular, neurologic, dermatologic, and cardiac systems. Cardiomyopathy seen in GSD IV is also heterogeneous and its appearance on cardiac magnetic resonance imaging (CMR) is rarely described. Read More

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http://dx.doi.org/10.1093/ehjcr/ytaa078DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7319828PMC

Overexpression of klotho suppresses growth and pulmonary metastasis of osteosarcoma in vivo.

Genet Mol Biol 2020 ;43(2):e20190229

Shanghai Fengxian District Central Hospital, Department of Orthopedics, Shanghai, China.

Klotho is originally discovered as an anti-aging gene and knock-out of klotho accelerates aging in mice. Subsequent studies support the anti-carcinogenesis role of klotho in a variety of human malignancies. The present study investigated the role of klotho on growth and metastasis of osteosarcoma cells. Read More

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http://dx.doi.org/10.1590/1678-4685-GMB-2019-0229DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263425PMC
January 2020

[From glycogen to lipid droplet: an intimate connection in the brown adipocyte].

Med Sci (Paris) 2020 Jun-Jul;36(6-7):577-579. Epub 2020 Jul 2.

Université de Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1011- Institut Européen de Génomique du Diabète (EGID), 1 rue du Pr Calmette, F-59019 Lille, France.

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http://dx.doi.org/10.1051/medsci/2020102DOI Listing

A Dual Inhibitor of DYRK1A and GSK3β for β-Cell Proliferation: Aminopyrazine Derivative GNF4877.

ChemMedChem 2020 Jul 2. Epub 2020 Jul 2.

Genomics Institute of the Novartis Research Foundation (GNF), 10675 John Jay Hopkins Drive, San Diego, CA 92121, USA.

Loss of β-cell mass and function can lead to insufficient insulin levels and ultimately to hyperglycemia and diabetes mellitus. The mainstream treatment approach involves regulation of insulin levels; however, approaches intended to increase β-cell mass are less developed. Promoting β-cell proliferation with low-molecular-weight inhibitors of dual-specificity tyrosine-regulated kinase 1A (DYRK1A) offers the potential to treat diabetes with oral therapies by restoring β-cell mass, insulin content and glycemic control. Read More

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http://dx.doi.org/10.1002/cmdc.202000183DOI Listing
July 2020
2.968 Impact Factor

Curcumin Protects Osteoblasts From Oxidative Stress-Induced Dysfunction via GSK3β-Nrf2 Signaling Pathway.

Front Bioeng Biotechnol 2020 16;8:625. Epub 2020 Jun 16.

Department of Oral and Maxillofacial Surgary/Pathology, Amsterdam UMC and Academic Centre for Dentistry Amsterdam, Amsterdam Movement Science, Vrije Universitetit Amsterdam, Amsterdam, Netherlands.

Osteoblasts dysfunction, induced by oxidative stress (OS), is one of major pathological mechanisms for osteoporosis. Curcumin (Cur), a bioactive antioxidant compound, isolated from Curcumin longa L, was regarded as a strong reactive oxygen species (ROS) scavenger. However, it remains unveiled whether Cur can prevent osteoblasts from OS-induced dysfunction. Read More

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http://dx.doi.org/10.3389/fbioe.2020.00625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7308455PMC

miR-34a Regulates the Activity of HIF-1a and P53 Signaling Pathways by Promoting GLUT1 in Genetically Improved Farmed Tilapia (GIFT, ) Under Hypoxia Stress.

Front Physiol 2020 16;11:670. Epub 2020 Jun 16.

Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.

In fish under hypoxia stress, homeostasis can become imbalanced, leading to tissue and organ damage and decreased survival. Therefore, it is useful to explore the molecular and physiological regulation mechanisms that function in fish under hypoxia stress. The microRNA miR-34a is involved in fat and glycogen metabolism, and in apoptosis. Read More

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http://dx.doi.org/10.3389/fphys.2020.00670DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7308589PMC

Molecular Evidence that Regulates the Development and Physiological Metabolism of .

Int J Mol Sci 2020 Jun 29;21(13). Epub 2020 Jun 29.

Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China.

Ashmead (Hymenoptera, Braconidae) is an endophagous parasitoid and Glover (Hemiptera, Aphididae) is a major pest in cotton. The relationship between insect host-parasitoids and their hosts involves complex physiological, biochemical and genetic interactions. This study examines changes in the development and physiological metabolism of regulated by . Read More

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http://dx.doi.org/10.3390/ijms21134610DOI Listing

Genetic loss of AMPK-glycogen binding destabilizes AMPK and disrupts metabolism.

Mol Metab 2020 Jun 28:101048. Epub 2020 Jun 28.

Exercise and Nutrition Research Program, Mary MacKillop Institute for Health Research, Australian Catholic University, Level 5, 215 Spring Street, Melbourne, Victoria, 3000, Australia. Electronic address:

Objective: Glycogen is a major energy reserve in liver and skeletal muscle. The master metabolic regulator AMP-activated protein kinase (AMPK) associates with glycogen via its regulatory β subunit carbohydrate-binding module (CBM). However, the physiological role of AMPK-glycogen binding in energy homeostasis has not been investigated in vivo. Read More

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http://dx.doi.org/10.1016/j.molmet.2020.101048DOI Listing

[Stable Nitrite Accumulation and Phosphorus Removal from High-nitrate and Municipal Wastewaters in a Combined Process of Partial Denitrification and Denitrifying Phosphorus Removal (PD-DPR)].

Huan Jing Ke Xue 2020 Mar;41(3):1384-1392

School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China.

In this study, a novel process combining partial denitrification (PD, NO-N→NO-N) and denitrifying phosphorus removal (DPR) in an anaerobic-anoxic-aerobic sequencing batch reactor (SBR) was developed. By comprehensively controlling the influent C/N ratio, anaerobic drainage ratio, and anoxic duration, the nitrite accumulation and phosphorus removal performance of a system treating high-strength nitrate and municipal wastewaters was investigated. The results showed that, after 140 days, the nitrate-to-nitrite transformation ratio (NTR) was 80. Read More

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http://dx.doi.org/10.13227/j.hjkx.201909251DOI Listing

Antifatigue and antihypoxia activities of oligosaccharides and polysaccharides from Codonopsis pilosula in mice.

Food Funct 2020 Jul 1. Epub 2020 Jul 1.

Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine; The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Shanghai R&D Center for Standardization of Chinese Medicines, 1200 Cailun Road, Shanghai 201203, China.

Codonopsis pilosula is a traditional Chinese medicine and food supplement that is widely used in China. This study aimed to investigate the antifatigue and antihypoxia activities of different extracts and fractions from C. pilosula, including ethanol extract (ETH), water extract (WAT), polysaccharides (POL), inulin (INU) and oligosaccharides (OLI). Read More

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http://dx.doi.org/10.1039/d0fo00468eDOI Listing

Increased Expression of p-GSK3β Predicts Poor Survival in T -III/IV Stage OSCC Patients.

In Vivo 2020 Jul-Aug;34(4):1805-1809

Department of Surgical Pathology, Changhua Christian Hospital, Changhua, Taiwan, R.O.C.

Background/aim: Glycogen synthase kinase 3 beta (GSK3-β) acts either as a tumor suppressor or an oncogene in various human cancers. The present study aimed to investigate the expression and activity of p-GSK3-β (Ser9) in oral cancer patients.

Materials And Methods: We investigated the levels of p-GSK3β in 152 oral cancer tissues by immunohistochemistry, and explored their prognostic impact. Read More

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http://dx.doi.org/10.21873/invivo.11975DOI Listing

Counteracting role of nuclear factor erythroid 2-related factor 2 pathway in Alzheimer's disease.

Biomed Pharmacother 2020 Jun 27;129:110373. Epub 2020 Jun 27.

Chitkara College of Pharmacy, Chitkara University, Punjab, India. Electronic address:

A salient pathological features in Alzheimer's disease includes redox impairment and neuroinflammation. Nuclear factor erythroid 2-related factor 2 (Nrf2) and Nuclear factor kappa B (NF-ҡB) are the two key transcription factors that regulate cellular responses to redox impairment and neuroinflammation respectively. An effective way to confer neuroprotection in central nervous system (CNS) is the activation of a transcription factor i. Read More

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http://dx.doi.org/10.1016/j.biopha.2020.110373DOI Listing

Paradoxical activation of AMPK by glucose drives selective EP300 activity in colorectal cancer.

PLoS Biol 2020 Jun 30;18(6):e3000732. Epub 2020 Jun 30.

Area of Physiology, Faculty of Health Sciences, University Rey Juan Carlos, Alcorcón, Madrid, Spain.

Coordination of gene expression with nutrient availability supports proliferation and homeostasis and is shaped by protein acetylation. Yet how physiological/pathological signals link acetylation to specific gene expression programs and whether such responses are cell-type-specific is unclear. AMP-activated protein kinase (AMPK) is a key energy sensor, activated by glucose limitation to resolve nutrient supply-demand imbalances, critical for diabetes and cancer. Read More

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http://dx.doi.org/10.1371/journal.pbio.3000732DOI Listing

The effects of aqueous extract of Maca on energy metabolism and immunoregulation.

Eur J Med Res 2020 Jun 29;25(1):24. Epub 2020 Jun 29.

School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, No. 11, East Road, North 3rd Ring Road, Chaoyang District, Beijing, 100029, China.

Background: In the present work, we investigated the effects of aqueous extract of Maca (AEM) on energy metabolism and immunoregulation in spleen-deficient mice.

Method: We established a cyclophosphamide-induced spleen-deficiency model with ginseng, a herb that strengthens splenic function, as a control. Sixty male Kunming mice were randomly divided among 5 groups: normal, model, ginseng control (1. Read More

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http://dx.doi.org/10.1186/s40001-020-00420-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322873PMC

Short communication: effects of dietary corticosterone on the central AMPK signaling pathway in broiler chickens.

J Anim Sci 2020 Jun 30. Epub 2020 Jun 30.

Division Animal and Human Health Engineering, Department of Biosystems, KU Leuven, Kasteelpark Arenberg, Leuven, Belgium.

Glucocorticoids (GCs) induce the activation of the central adenosine 5'-monophosphate-activated protein kinase (AMPK) signaling pathway in birds. In this study, we aimed to investigate the effects of corticosterone (CORT) supplemented in diet on the central AMPK signaling pathway in broilers. Average daily gain (ADG) was reduced by CORT treatment, and average daily feed intake (ADFI) remained unchanged. Read More

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http://dx.doi.org/10.1093/jas/skaa202DOI Listing

Sex-specific acclimation of A2 noradrenergic neuron dopamine-β-hydroxylase and estrogen receptor variant protein and 5'-AMP-Activated protein kinase reactivity to recurring hypoglycemia in rat.

J Chem Neuroanat 2020 Jun 26;109:101845. Epub 2020 Jun 26.

School of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy, University of Louisiana Monroe, Monroe, LA, 71201, United States.

Hindbrain estrogen receptors (ER) impose sex-dimorphic control of counter-regulatory hormone and hypothalamic glucoregulatory transmitter and glycogen metabolic responses to hypoglycemia. A2 noradrenergic neurons are estradiol- and metabolic-sensitive. Estradiol controls dopamine-beta-hydroxylase (DBH) protein habituation to recurrent insulin-induced hypoglycemia (RIIH) in females. Read More

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http://dx.doi.org/10.1016/j.jchemneu.2020.101845DOI Listing

Effect of low energy availability during three consecutive days of endurance training on iron metabolism in male long distance runners.

Physiol Rep 2020 Jun;8(12):e14494

Japan Institute of Sports Sciences, Kitaku, Tokyo, Japan.

We investigated the effect of low energy availability (LEA) during three consecutive days of endurance training on muscle glycogen content and iron metabolism. Six male long distance runners completed three consecutive days of endurance training under LEA or neutral energy availability (NEA) conditions. Energy availability was set at 20 kcal/kg fat-free mass (FFM)/day for LEA and 45 kcal/kg FFM/day for NEA. Read More

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http://dx.doi.org/10.14814/phy2.14494DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322269PMC

Muscle Fatigue-Alleviating Effects of a Prescription Composed of Polygonati Rhizoma and Notoginseng Radix et Rhizoma.

Biomed Res Int 2020 8;2020:3963045. Epub 2020 Jun 8.

College of Pharmaceutical Science, Yunnan University of Chinese Medicine, 1076 Yuhua Road, Kunming 650500, China.

Long-term muscle fatigue is a major cause of injury. Drugs/nutrients from herbal medicines that prevent fatigue remain a major research focus. In China, a prescription composed of Polygonati Rhizoma and Notoginseng Radix et Rhizoma has been commonly used as a herb and food nutrient, providing protection against fatigue in the clinic. Read More

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http://dx.doi.org/10.1155/2020/3963045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7301163PMC

Hydrogen: An Endogenous Regulator of Liver Homeostasis.

Front Pharmacol 2020 11;11:877. Epub 2020 Jun 11.

Department of Traditional Chinese Medicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Basic and clinical studies have shown that hydrogen (H), the lightest gas in the air, has significant biological effects of anti-oxidation, anti-inflammation, and anti-apoptosis. The mammalian cells have no abilities to produce H due to lack of the expression of hydrogenase. The endogenous H in human body is mainly produced by anaerobic bacteria, such as and , in gut and other organs through the reversible oxidation reaction of 2 H + 2 e ⇌ H. Read More

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http://dx.doi.org/10.3389/fphar.2020.00877DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7301907PMC

Inhibition of GSK3β and RIP1K Attenuates Glial Scar Formation Induced by Ischemic Stroke Reduction of Inflammatory Cytokine Production.

Front Pharmacol 2020 12;11:812. Epub 2020 Jun 12.

Jiangsu Key Laboratory of Neuropsychiatric Diseases and College of Pharmaceutical Sciences, Department of Pharmacology and Laboratory of Cerebrovascular Pharmacology, College of Pharmaceutical Science, Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, School of Public Health, Soochow University, Suzhou, China.

In the chronic phase following ischemic stroke, glial scars can prevent axonal regeneration and the intensification of inflammation. The protective effect of inhibition of glycogen synthase kinase-3β (GSK3β) or receptor-interacting protein 1 kinase (RIP1K) on ischemic stroke has been previously reported. The current study examined the effects of RIP1K and GSK3β on ischemic stroke-induced glial scar formation. Read More

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http://dx.doi.org/10.3389/fphar.2020.00812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7303311PMC

Cardiac phenotype in glycogen storage disease type XV: a rare cardiomyopathy to bear in mind.

Rev Esp Cardiol (Engl Ed) 2020 Jun 25. Epub 2020 Jun 25.

Unidad de Cardiopatías Familiares, Muerte Súbita y Mecanismos de Enfermedad (CaFaMuSMe), Instituto de Investigación Sanitaria La Fe, Valencia, Spain; Centro de Investigación Biomédica en Red Enfermedades Cardiovasculares (CIBERCV), Madrid, Spain; Servicio de Cardiología, Hospital Universitario y Politécnico La Fe, Valencia, Spain. Electronic address:

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http://dx.doi.org/10.1016/j.rec.2020.05.020DOI Listing

Impacts of salicylic acid in Mytilus galloprovincialis exposed to warming conditions.

Environ Toxicol Pharmacol 2020 Jun 25;80:103448. Epub 2020 Jun 25.

Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Italy. Electronic address:

While many studies have been conducted on drug-inducing alterations in the aquatic environment, little is known about their interaction with climate change, such as rising temperatures. To increase knowledge on this topic, Mytilus galloprovincialis mussels were exposed to two different temperatures 17 ± 1 °C (control) and 21 ± 1 °C in the absence and presence of salicylic acid (SA) (4 mg/L) for 28 days. Salicylic acid in the water and tissues was measured and its impact reported through biomarker responses including: energy metabolism (electron transport system (ETS) activity, glycogen (GLY), protein (PROT) and lipids (LIP) contents), oxidative stress markers (activity of the enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)), glutathione balance between the reduced and the oxidized forms (GSH/GSSG), and damage to membrane lipids (lipid peroxidation - LPO). Read More

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http://dx.doi.org/10.1016/j.etap.2020.103448DOI Listing

Loss of CLN3, the gene mutated in juvenile neuronal ceroid lipofuscinosis, leads to metabolic impairment and autophagy induction in retina pigment epithelium.

Biochim Biophys Acta Mol Basis Dis 2020 Jun 24:165883. Epub 2020 Jun 24.

Department of Ophthalmology and Visual Sciences, University of Kentucky, Lexington, KY, United States; Markey Cancer Center, University of Kentucky, Lexington, KY, United States. Electronic address:

Juvenile neuronal ceroid lipofuscinosis (JNCL, aka. juvenile Batten disease or CLN3 disease) is a lysosomal storage disease characterized by progressive blindness, seizures, cognitive and motor failures, and premature death. JNCL is caused by mutations in the Ceroid Lipofuscinosis, Neuronal 3 (CLN3) gene, whose function is unclear. Read More

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http://dx.doi.org/10.1016/j.bbadis.2020.165883DOI Listing

Hypothesis validation of rosiglitazone a potential inhibitor against glycogen synthase kinase-3β, for the management of multifaceted pathophysiology of the diabetic wound: An insilico study.

Med Hypotheses 2020 Jun 13;144:109990. Epub 2020 Jun 13.

Department of Pharmaceutics, Max Institute of Pharmaceutical Sciences, Khammam, Telangana 507318, India. Electronic address:

Diabetic wound (DW) is a major complication of diabetes mellitus that often ends up with amputation of the concerned organ. The current therapies for DW include glucose regulation, wound debridement, pressure off-loading, surgeries, hyperbaric oxygen therapy, maggot therapy, and so on. However, the majority are not meeting all the prerequisites of DW because of extensive pathology and cost associated with the strategies. Read More

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http://dx.doi.org/10.1016/j.mehy.2020.109990DOI Listing

Glycogen as an advantageous polymer carrier in cancer theranostics: Straightforward in vivo evidence.

Sci Rep 2020 Jun 26;10(1):10411. Epub 2020 Jun 26.

MR Unit, Department of Radiodiagnostic and Interventional Radiology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.

As a natural polysaccharide polymer, glycogen possesses suitable properties for use as a nanoparticle carrier in cancer theranostics. Not only it is inherently biocompatible, it can also be easily chemically modified with various moieties. Synthetic glycogen conjugates can passively accumulate in tumours due to enhanced permeability of tumour vessels and limited lymphatic drainage (the EPR effect). Read More

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http://dx.doi.org/10.1038/s41598-020-67277-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7320016PMC

Abscisic acid enriched fig extract promotes insulin sensitivity by decreasing systemic inflammation and activating LANCL2 in skeletal muscle.

Sci Rep 2020 Jun 26;10(1):10463. Epub 2020 Jun 26.

NIMML Institute, Blacksburg, VA, 24060, USA.

Abscisic acid is a phytohormone found in fruits and vegetables and is endogenously produced in mammals. In humans and mice, lanthionine synthetase C-like 2 (LANCL2) has been characterized as the natural receptor for ABA. Herein, we characterize the efficacy of a fig fruit extract of ABA in promoting glycemic control. Read More

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http://dx.doi.org/10.1038/s41598-020-67300-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7319979PMC

Regulation of Keap-1/Nrf2 Signaling Pathway Is Activated by Oxidative Stress in Patients with Premature Rupture of Membranes.

Med Sci Monit 2020 Jun 26;26:e921757. Epub 2020 Jun 26.

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Shandong First Medical University, Jinan, Shandong, China (mainland).

BACKGROUND The potential mechanisms underlying premature rupture of membrane (PROM) is still unknown. The aim of this study was to determine the role of Keap-1/Nrf2 signaling pathway activation by oxidative stress in patients with preterm premature rupture of membranes. MATERIAL AND METHODS Placental tissues from preterm premature rupture of membranes (PPROM) (n=20), full-term premature rupture of membranes (FPROM) (n=20), and normal-term births (n=20) were collected and amniotic tissues were separated from the placental tissues from pregnant women at Shandong Provincial Qianfoshan Hospital. Read More

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http://dx.doi.org/10.12659/MSM.921757DOI Listing

Skeletal muscle AMPK is not activated during 2 hours of moderate intensity exercise at ∼65% VO peak in endurance trained men.

J Physiol 2020 Jun 26. Epub 2020 Jun 26.

Department of Physiology, University of Melbourne, Victoria, 3010, Australia.

Key Points: AMP-activated protein kinase (AMPK) is considered a major regulator of skeletal muscle metabolism during exercise. However, we previously showed that although AMPK activity increases 8-10 fold during ∼120 min of exercise at ∼65% VO peak in untrained individuals, there is no increase in these individuals after only 10 days of exercise training (longitudinal study). In a cross-sectional study we show that there is also a lack of activation of skeletal muscle AMPK during 120 min of cycling exercise at 65% VO peak in endurance-trained individuals. Read More

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http://dx.doi.org/10.1113/JP277619DOI Listing

In silico screening of glycogen synthase kinase-3β targeted ligands against acetylcholinesterase and its probable relevance to Alzheimer's disease.

J Biomol Struct Dyn 2020 Jun 26:1-10. Epub 2020 Jun 26.

Department of Biochemistry, Centre for Research and Development, PRIST University, Vallam, Thanjavur, Tamil Nadu, India.

Alzheimer's disease (AD) is a growing global health concern that affects 10% of the population aged above 65 years. A growing body of evidence indicates that multi-targeted drugs might be useful therapeutic options owing to the heterogeneity of AD pathology. The current study exploited advanced computational biology tools to identify ligands that might display effective binding to two protein targets in the context of AD. Read More

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http://dx.doi.org/10.1080/07391102.2020.1784796DOI Listing