64,797 results match your criteria Glycogen Storage Diseases Types I-VII


fraction inhibits the invasion and metastasis of lung cancer cells through the protein kinase B/glycogen synthase kinase 3β/β-catenin signaling pathway.

Oncol Lett 2018 Dec 27;16(6):6930-6939. Epub 2018 Sep 27.

Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou, Guangdong 510632, P.R. China.

is widely used as a traditional Chinese medicine health supplement, and is also used in the development of anticancer agents. In our previous studies, it was revealed that fraction (CMF) possessed an antitumor effect against K562 cells , induced apoptosis and caused cell cycle arrest in the S phase. The published results also demonstrated that CMF-induced apoptosis was involved in mitochondrial dysfunction. Read More

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December 2018

The combination of bFGF and CHIR99021 maintains stable self-renewal of mouse adult retinal progenitor cells.

Stem Cell Res Ther 2018 Dec 13;9(1):346. Epub 2018 Dec 13.

Department of Ophthalmology of Shanghai Tenth People's Hospital, and Tongji Eye Institute, Tongji University School of Medicine, Shanghai, 200072, China.

Background: Millions of people are affected with retinal diseases that eventually cause blindness, and retinal progenitor cell (RPC) transplantation is a promising therapeutic avenue. However, RPC expansion and the underlying regulation mechanisms remain elusive.

Methods: Adult mouse neural RPCs (mNRPCs) were isolated and amplified with the combination of basic fibroblast growth factor (bFGF) and glycogen synthase kinase 3 (GSK3) inhibitor CHIR99021. Read More

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December 2018

In Vitro and In Vivo Functional Characterization of Essence of Chicken as An Ergogenic Aid.

Nutrients 2018 Dec 7;10(12). Epub 2018 Dec 7.

Graduate Institute of Sports Science, National Taiwan Sports University, Taoyuan City 33301, Taiwan.

Essence of chicken is a popular Asian nutritional supplement that is often taken to improve metabolism and general health. Although used as a traditional remedy for combating fatigue and general health, there has been few studies investigating the ergogenic properties of chicken essence and its associated mechanism. We conducted a study to investigate the anti-fatigue and anti-oxidant properties of essence of chicken (EC) after exercise. Read More

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December 2018

Early effects of eccentric contractions on muscle glucose uptake.

J Appl Physiol (1985) 2018 Dec 13. Epub 2018 Dec 13.

Department of Public Health, University of Aarhus, Denmark.

Muscle damaging eccentric exercise impairs muscle glucose uptake several hours to days after exercise. Little, however, is known about the acute effects of eccentric exercise on contraction- and insulin-induced glucose uptake. This study compares glucose uptake rates in the first hours following eccentric, concentric, and isometric contractions with and without insulin present. Read More

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December 2018

sFRP1 exerts effects on gastric cancer cells through GSK3β/Rac1‑mediated restraint of TGFβ/Smad3 signaling.

Oncol Rep 2018 Oct 31. Epub 2018 Oct 31.

Department of Gastrointestinal Surgery, Guangzhou First People's Hospital, The Second Affiliated Hospital of South China University of Technology, Guangzhou, Guangdong 510180, P.R. China.

Secreted frizzled‑related protein 1 (sFRP1) is an inhibitor of canonical Wnt signaling; however, previous studies have determined a tumor‑promoting function of sFRP1 in a number of different cancer types. A previous study demonstrated that sFRP1 overexpression was associated with an aggressive phenotype and the activation of transforming growth factor β (TGFβ) signaling. sFRP1 overexpression and sFRP1 knockdown cell models were established. Read More

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October 2018

Serous cystadenoma of pancreas: A clinicopathologic experience of 23 cases from a major tertiary care center.

Rare Tumors 2018 5;10:2036361318809183. Epub 2018 Nov 5.

Section of Histopathology, Department of Pathology and Laboratory Medicine, Aga Khan University Hospital, Karachi, Pakistan.

Background: Serous cystadenomas of pancreas are rare benign epithelial neoplasms, which predominantly occur in the pancreatic body and tail of elderly females. Majority of these tumors have microcystic appearance. Macrocystic and solid variants have also been described. Read More

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November 2018

Ellagic acid ameliorates learning and memory impairment in APP/PS1 transgenic mice via inhibition of β-amyloid production and tau hyperphosphorylation.

Exp Ther Med 2018 Dec 15;16(6):4951-4958. Epub 2018 Oct 15.

Key Laboratory of Acupuncture Clinical Neurobiology (Encephalopathy), Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, P.R. China.

β-amyloid (Aβ) aggregation and tau hyperphosphorylation are considered to be the primary pathological hallmarks of Alzheimer's disease (AD). Targeted inhibition of these pathological processes may provide effective treatments for AD. Accumulating evidence has demonstrated that ellagic acid (EA) exerts neuroprotective effects in several diseases. Read More

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December 2018

PTEN in propofol-induced insulin resistance in mouse primary hepatocytes.

Exp Ther Med 2018 Dec 1;16(6):4831-4835. Epub 2018 Oct 1.

Department of Anesthesiology, Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Shenzhen, Guangdong 518000, P.R. China.

Propofol is the most common intravenous anesthetic agent used in clinical practice. Propofol can induce insulin resistance in mouse primary hepatocytes, however the molecular mechanism through which propofol acts remains largely unknown. Based on previous studies, it was hypothesized that phosphatase and tensin homolog (PTEN) is involved in propofol-mediated insulin resistance. Read More

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December 2018

Glycogen Synthase Kinase 3β Enhances Hepatitis C Virus Replication by Supporting miR-122.

Front Microbiol 2018 27;9:2949. Epub 2018 Nov 27.

Department of Internal Medicine 1, University Hospital Frankfurt, Frankfurt, Germany.

Hepatitis C virus (HCV) infection is associated with alterations in host lipid and insulin signaling cascades, which are partially explained by a dependence of the HCV life cycle on key molecules in these metabolic pathways. Yet, little is known on the role in the HCV life cycle of glycogen synthase kinase 3 (GSK3), one of the most important kinases in cellular metabolism. Therefore, the impact of GSK3 on the HCV life cycle was assessed in human hepatoma cell lines harboring subgenomic genotype 1b and 2a replicons or producing cell culture-derived HCV genotype 2a by exposure to synthetic GSK3 inhibitors, GSK3 gene silencing, overexpression of GSK3 constructs and immunofluorescence analyses. Read More

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November 2018

Trastuzumab induces PUMA-dependent apoptosis and inhibits tumor growth in gastric cancer.

FEBS Open Bio 2018 Dec 19;8(12):1911-1919. Epub 2018 Nov 19.

Department of Radiology Shanghai East Hospital Tongji University School of Medicine Shanghai China.

Gastric cancer (GC) is one of the most prevalent cancers worldwide. Trastuzumab has been approved for the treatment of metastatic GC, gastroesophageal junction cancer, and breast cancer. However, the mechanisms involved in trastuzumab-induced GC cell apoptosis remain largely unknown. Read More

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December 2018

Maternal Oxidative Stress, Placental Morphometry, and Fetal Growth in Diabetic Rats Exposed to Cigarette Smoke.

Reprod Sci 2018 Dec 6:1933719118815589. Epub 2018 Dec 6.

1 Laboratory of Experimental Research on Gynecology and Obstetrics, Botucatu Medical School, Sao Paulo State University (UNESP), Botucatu, São Paulo, Brazil.

The diabetic syndrome affects pregnancy, contributing to placental functional and structural disruptions and impaired fetal development, with many reports indicating tobacco-associated morbidity and perinatal mortality. In our study, an experimental rat model of diabetes and cigarette smoke exposure in pregnant rats was used to determine the impact of the combination of diabetes and exposure to cigarette smoke during pregnancy on maternal oxidative stress biomarkers and placental and fetal development. Diabetes was induced by streptozotocin, and dams were exposed to cigarette smoke by mainstream smoke generated by a mechanical smoking device and delivered into a chamber. Read More

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December 2018

Correction to: P2X7R-dependent regulation of glycogen synthase kinase 3β and claudin-18 in alveolar epithelial type I cells of mice lung.

Histochem Cell Biol 2018 Dec 11. Epub 2018 Dec 11.

Institute of Anatomy, Medical Faculty "Carl Gustav Carus", Technical University Dresden, Fetscherstr. 74, 01307, Dresden, Germany.

Correction to: Histochem Cell Biol (2016). Read More

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December 2018

Protein Kinase A and High-Osmolarity Glycerol Response Pathways Cooperatively Control Cell Wall Carbohydrate Mobilization in .

MBio 2018 Dec 11;9(6). Epub 2018 Dec 11.

Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil

mitogen-activated protein kinases (MAPKs) are involved in maintaining the normal morphology of the cell wall and providing resistance against cell wall-damaging agents. Upon cell wall stress, cell wall-related sugars need to be synthesized from carbohydrate storage compounds. Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC. Read More

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December 2018

Dexamethasone induces osteoblast apoptosis through ROS-PI3K/AKT/GSK3β signaling pathway.

Biomed Pharmacother 2018 Dec 8;110:602-608. Epub 2018 Dec 8.

Department of Orthopedics, Renmin Hospital of Wuhan University, Jiefang Road 238, Wuhan, Hubei, 430060, China. Electronic address:

Background: Osteoblasts play important roles in the process of osteogenesis and prevention of osteonecrosis. Dexamethasone (Dex), a type of glucocorticoids (GCs), induces apoptosis of osteoblasts and leads to the occurrence of non-traumatic osteonecrosis. This study aimed to explore the effects of phosphatidylinositol 3-kinase/Protein kinase 3 (PI3K/AKT) and glycogen synthase kinase 3β (GSK3β) on Dex-induced osteoblasts apoptosis. Read More

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December 2018
2 Reads
2.023 Impact Factor

Isolation and Expansion of Rat Hepatocytic Progenitor Cells.

Methods Mol Biol 2019 ;1905:29-41

Department of Tissue Development and Regeneration, Research Institute for Frontier Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan.

This protocol showed procedures to isolate and expand small hepatocytes (SHs), as hepatocytic progenitor cells, from a rat liver. SHs are identified as a subpopulation of mature hepatocytes in a healthy liver. SHs can proliferate to form colonies in serum-free medium on hyaluronic acid-coated dishes, of which cells show CD44 positivity (CD44 SHs). Read More

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January 2019

Cellular Metabolism at a Glance.

Exp Suppl 2018 ;109:3-27

Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal.

Metabolism is highly coordinated component of the cellular activity that involves sequential chemical transformations, within a so-called metabolic network. Through these coordinated actions, living organisms acquire energy and biosynthetic precursors to maintain cellular homeostasis and function. Metabolism relies on the breaking down of macromolecules to produce energy [catabolism] and/or intermediary metabolites that are then used to construct essential building blocks for macromolecule production [anabolism]. Read More

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January 2018

PI3K/Akt signaling transduction pathway, erythropoiesis and glycolysis in hypoxia (Review).

Mol Med Rep 2018 Dec 3. Epub 2018 Dec 3.

Department of Respiratory Medicine, Qinghai Provincial People's Hospital, Xining, Qinghai 810007, P.R. China.

The purpose of this review is to summarize the research progress of PI3K/Akt signaling pathway in erythropoiesis and glycolysis. Phosphatidylinositol‑4,5‑bisphosphate 3‑kinase (PI3K) is activated by numerous genes and leads to protein kinase B (Akt) binding to the cell membrane, with the help of phosphoinositide‑dependent kinase, in the PI3K/Akt signal transduction pathway. Threonine and serine phosphorylation contribute to Akt translocation from the cytoplasm to the nucleus and further mediates enzymatic biological effects, including those involved in cell proliferation, apoptosis inhibition, cell migration, vesicle transport and cell cancerous transformation. Read More

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December 2018

A potential role for GSK3beta in glucose-driven intrauterine catch-up growth in maternal obesity.

Endocrinology 2018 Dec 10. Epub 2018 Dec 10.

Department of Paediatrics and Adolescent Medicine, University Hospital of Cologne, Cologne, Germany.

Obesity and an unhealthy nutrition are on the rise and affect also women in childbearing age and hence, during pregnancy. Despite normal or even high birth weight the offspring suffers from long term metabolic risks. We hypothesized that fetal growth is disturbed during different intrauterine phases. Read More

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December 2018

Comparison of the Biochemical Composition and Nutritional Quality Between Diploid and Triploid Hong Kong Oysters, .

Front Physiol 2018 26;9:1674. Epub 2018 Nov 26.

Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.

This study is the first systematic comparison of the biochemical composition and nutritional quality between diploid and triploid Hong Kong oysters, . Results showed that in the reproductive season, the glycogen content in five tissues (gill, mantle, adductor muscle, labial palps and gonad) was significantly higher ( < 0.05) in triploids than in diploids, with odds ratios (ORs) of 96. Read More

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November 2018

IL-33 suppresses GSK-3β activation through an ST2-independent MyD88/TRAF6/RIP/PI3K/Akt pathway.

Heliyon 2018 Nov 26;4(11):e00971. Epub 2018 Nov 26.

Shanghai University of Traditional Chinese Medicine, Education College of Medicine, Osaka, 530-0047, Japan.

Aims: The present study was conducted to explore the effect of interleukin-33 (IL-33) on glycogen synthase kinase-3β (GSK-3β) activation involving Tau phosphorylation, a critical causative factor for Alzheimer's disease (AD).

Main Methods: Experiments were performed using PC-12 cells. Target proteins were knocked-down by transfecting with the siRNA for each protein. Read More

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November 2018

Biochemical Activity and Hypoglycemic Effects of L. Seeds Used in Armenian Traditional Medicine.

Biomed Res Int 2018 7;2018:4526352. Epub 2018 Nov 7.

Department of Biochemistry, Microbiology and Biotechnology, Faculty of Biology, Yerevan State University, 0025 Yerevan, Armenia.

Diabetes mellitus (DM) is a serious chronic metabolic disorder. Various diseases are being treated with medicinal plants and that is because of the less side effects of the current therapy. The diversity of plants in Armenia is due to the singularity of natural environment. Read More

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November 2018

Fine structure of the aggregate silk nodules in the orb-web spider .

Authors:
Myung-Jin Moon

Anim Cells Syst (Seoul) 2018 14;22(6):421-428. Epub 2018 Nov 14.

Department of Biological Sciences, Dankook University, Cheonan, South Korea.

Among the triad spinning units for capture thread producing system in the orb-web spiders, aqueous gluey substances are produced from two pairs of aggregate silk glands (ASG). Although biochemical analysis of glue substance is produced by way of their passage through the ASG, its structural modifications in the nodular area have been nearly neglected till now. This paper focused on identifying the fine structural characteristics of the aggregate nodules in the golden orb-web spider using both of light and transmission electron microscopes. Read More

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November 2018

Follicle-stimulating hormone inhibits cervical cancer via NF-κB pathway.

Onco Targets Ther 2018 14;11:8107-8115. Epub 2018 Nov 14.

The Institute of Audiology and Speech Science of Xuzhou Medical College, Xuzhou 221002, People's Republic of China,

Background: Follicle-stimulating hormone (FSH) has multiple biological functions. It is currently considered that FSH can inhibit cervical cancer, and our aim was to explore the underlying molecular mechanisms.

Materials And Methods: An in vivo experiment using nude mice injected with HeLa cells was performed. Read More

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November 2018

Effects of Ketone Bodies on Endurance Exercise.

Curr Sports Med Rep 2018 Dec;17(12):444-453

Unit of Endocrinology, Department of Movement, Human and Health Sciences, Università degli Studi di Roma "Foro Italico", Rome, ITALY.

Priorities for every athlete include improving endurance performance, optimizing training, nutrition, and recovery. Nutritional strategies are crucial to support athletes to perform at the highest level, and considering that muscular and hepatic glycogen stores are limited, alternative strategies to maximize fat metabolism have been suggested. A ketogenic diet has been proposed as a possible method of providing metabolic fuel during prolonged periods of exercise. Read More

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December 2018

Physiological insights into largemouth bass (Micropterus salmoides) survival during long-term exposure to high environmental ammonia.

Aquat Toxicol 2018 Nov 30;207:72-82. Epub 2018 Nov 30.

Department of Aquaculture and Fisheries, University of Arkansas at Pine Bluff, 1200 North University Drive, Pine Bluff, 71601, AR, USA. Electronic address:

Waterborne ammonia is an environmental pollutant that is toxic to all aquatic animals. However, ammonia induced toxicity as well as compensatory mechanisms to defend against high environmental ammonia (HEA) are not well documented at present for largemouth bass (Micropterus salmoides), a high value fish for culture and sport fisheries in the United States. To provide primary information on the sensitivity of this species to ammonia toxicity, a 96 h-LC test was conducted. Read More

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November 2018

Polycyclic maleimide-based derivatives as first dual modulators of neuronal calcium channels and GSK-3β for Alzheimer's disease treatment.

Eur J Med Chem 2018 Dec 3;163:394-402. Epub 2018 Dec 3.

Department of Pharmacy and Biotechnology, Alma Mater Studiorum-University of Bologna, Via Belmeloro 6, 40126, Bologna, Italy. Electronic address:

Current healthcare has significantly increased the average life expectancy, leading to a consequently greater incidence of age-related diseases, such as Alzheimer's disease. Following a multitarget approach, in this paper a series of polycyclic maleimide-based derivatives were designed and synthesized aimed at simultaneously modulate neuronal calcium channels and glycogen synthase kinase 3-beta (GSK-3β), validated targets to combat Alzheimer' disease. Different structural modifications were performed on the polycyclic scaffold in order to investigate the structure-activity relationships and compound 10 emerged as a promising non-toxic lead compound, endowed with calcium modulating brain-addressed properties and significant GSK-3β inhibitory activity. Read More

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December 2018

Hypoglycemic activity of the extracts of Belamcanda chinensis leaves (BCLE) on KK-A mice.

Biomed Pharmacother 2018 Dec 5;110:449-455. Epub 2018 Dec 5.

BIT&GS Technologies Co. Ltd., PR China. Electronic address:

The extract of Belamcanda chinensis (L.) leaves (BCLE) is a traditional Chinese herbal medicine for the treatment of hyperglycemia in Hainan province, South China. In this study, the effects of BCLE on obese diabetes were investigated using the KK-A mice. Read More

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December 2018

Benzo(a)pyrene degradation by cytochrome P450 hydroxylase and the functional metabolism network of Bacillus thuringiensis.

J Hazard Mater 2018 Dec 3;366:329-337. Epub 2018 Dec 3.

Guangdong Key Laboratory of Environmental Pollution and Health, School of Environment, Jinan University, Guangzhou, 510632, China. Electronic address:

The relationship between benzo(a)pyrene biodegradation and certain target biomolecules has been investigated. To regulate the degradation process, the associated metabolism network must be clarified. To this end, benzo(a)pyrene degradation, carbon substrate metabolism and exometabolomic mechanism of Bacillus thuringiensis were analyzed. Read More

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December 2018

Minocycline Attenuates Ethanol-induced Cell Death and Microglial Activation in the Developing Spinal Cord.

Alcohol 2018 Dec 7. Epub 2018 Dec 7.

Department of Pharmacology and Nutritional Sciences, University of Kentucky College of Medicine, Lexington, KY 40536, USA; Lexington VA Health Care System, Research & Development, 1101 Veterans Drive, Lexington, Kentucky 40502, USA. Electronic address:

Developmental exposure to ethanol may cause fetal alcohol spectrum disorders (FASD), and immature central nervous system (CNS) is particularly vulnerable to ethanol. In addition to the developing brain, we previously showed that ethanol also caused neuroapoptosis, microglial activation, and neuroinflammation in the spinal cord. Minocycline is an antibiotic that inhibits microglial activation and alleviates neuroinflammation. Read More

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December 2018

Gene expression of AMPA, kainate, and NMDA receptor subunits in the pigeon spinal cord.

J Chem Neuroanat 2018 Dec 7. Epub 2018 Dec 7.

Laboratory of Veterinary Anatomy, Faculty of Applied Biological Sciences, Gifu University, Yanagido, Gifu 501-1193, Japan.

Glutamatergic neurons are widely distributed in gray matter across the four segments of the pigeon spinal cord. Postsynaptic neurons containing ionotropic glutamate receptors (iGluRs), however, have been elucidated in only some AMPA subunits. This study examined the localization of postsynaptic neurons having three types of iGluRs in the pigeon spinal cord. Read More

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December 2018

Alcohol-induced ketonemia is associated with lowering of blood glucose, downregulation of gluconeogenic genes, and depletion of hepatic glycogen in type 2 diabetic db/db mice.

Biochem Pharmacol 2018 Dec 7. Epub 2018 Dec 7.

Center for Pharmacy and Experimental Therapeutics, University of Georgia College of Pharmacy, Augusta, Georgia, USA; Charlie Norwood VA Medical Center, Augusta, Georgia, USA; Vascular Biology Center, Department of Pharmacology and Toxicology, Georgia Regents University, Augusta, Georgia, USA; Department of Medicine, Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA. Electronic address:

Alcoholic ketoacidosis and diabetic ketoacidosis are life-threatening complications that share the characteristic features of high anion gap metabolic acidosis. Ketoacidosis is attributed in part to the massive release of ketone bodies (e.g. Read More

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December 2018

Zinc and p53 disrupt mitochondrial binding of HK2 by phosphorylating VDAC1.

Exp Cell Res 2018 Dec 4. Epub 2018 Dec 4.

Department of Pathophysiology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin, China. Electronic address:

Many cell death regulators physically or functionally interact with metabolic enzymes. These interactions provide insights into mechanisms of anticancer treatments from the perspective of tumor cell metabolism and apoptosis. Recent studies have shown that zinc and p53 not only induce tumor cell apoptosis, but also regulate tumor cell metabolism. Read More

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December 2018

Short-term and long-term ketogenic diet therapy and the addition of exercise have differential impacts on metabolic gene expression in the mouse energy-consuming organs heart and skeletal muscle.

Nutr Res 2018 Dec 19;60:77-86. Epub 2018 Sep 19.

Department of Nutrition and Food Science, Graduate School of Humanities and Sciences, Ochanomizu University, 2-1-1 Otsuka, Bunkyo, Tokyo 112-8610, Japan; The Institute for Human Life Innovation, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610, Japan. Electronic address:

Although a ketogenic diet (KD) is used to treat various metabolic diseases, the organ-specific metabolic changes that occur in response to a KD remain unclear. Therefore, we tested the hypothesis that duration of KD consumption and regular exercise in addition to KD consumption affect metabolic fuel selection at gene levels in heart and skeletal muscle. Six-week-old male C57BL/6J mice were divided into 2 groups, one fed a standard diet and the other fed a KD, and maintained for either 4 weeks (short term) or 12 weeks (long term). Read More

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December 2018

Pretreatment of rAAV-Mediated Expression of Myostatin Propeptide Lowers Type 2 Diabetes Incidence in C57BL/6 Mice on High-fat Diet.

Hum Gene Ther 2018 Dec 10. Epub 2018 Dec 10.

Division of Cardiology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei Key Laboratory of Genetics and Molecular Mechanisms of Cardiological Disorders (Huazhong University of Science and Technology) , 1095# Jiefang Ave., Wuhan 430030, P.R. China , Wuhan, China , 430030 ;

Myostatin, a negative modulator of muscle growth, has been considered as a potential target for treatment of type 2 diabetes. In our previous work, we found that myostatin inhibition by adeno-associated virus (AAV) mediated gene delivery of myostatin propeptide (MPRO) could improve muscle mass and achieve therapeutic effects on glucose regulation and lipid metabolism in db/db mice. In this study, we investigated whether pre-intervention of rAAV-mediated expression of MPRO could lower the incidence of type 2 diabetes. Read More

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December 2018

Preclinical Safety Evaluation of HIV-1 gp120 Responsive Microbicide Delivery System in C57BL/6 Female Mice.

Mol Pharm 2018 Dec 10. Epub 2018 Dec 10.

Many novel vaginal/rectal microbicides formulations failed clinically due to safety concerns, indicating the need for the early investigation of lead microbicide formulations. In this study, the preclinical safety of an HIV-1 gp120 and mannose responsive microbicide delivery system (MRP) is evaluated in C57BL/6 mice. MRP was engineered through the layer-by-layer coating of calcium carbonate (CaCO3) with Canavalia ensiformis lectin (Con A) and glycogen. Read More

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December 2018

Novel metabolic disorders in skeletal muscle of Lipodystrophic Bscl2/Seipin deficient mice.

Mol Cell Endocrinol 2018 Dec 4. Epub 2018 Dec 4.

Department of Physiology, Medical College of Georgia at Augusta University, Augusta, GA, 30912, USA. Electronic address:

Bscl2 mice recapitulate many of the major metabolic manifestations in Berardinelli-Seip congenital lipodystrophy type 2 (BSCL2) individuals, including lipodystrophy, hepatosteatosis, muscular hypertrophy, and insulin resistance. Metabolic defects in Bscl2 mice with regard to glucose and lipid metabolism in skeletal muscle have never been investigated. Here, we identified Bscl2 mice displayed reduced intramyocellular triglyceride (IMTG) content but increased glycogen storage predominantly in oxidative type I soleus muscle (SM). Read More

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December 2018
5 Reads

Differential effects of type 1 diabetes mellitus and subsequent osteoblastic β-catenin activation on trabecular and cortical bone in a mouse model.

Exp Mol Med 2018 Dec 5;50(12):158. Epub 2018 Dec 5.

State Key Laboratory of Trauma, Burn and Combined Injury, Department of War Wound Rescue Skills Training, Base of Army Health Service Training, Army Medical University, 400038, ChongQing, China.

Type 1 diabetes mellitus (T1DM) is a pathological condition associated with osteopenia. WNT/β-catenin signaling is implicated in this process. Trabecular and cortical bone respond differently to WNT/β-catenin signaling in healthy mice. Read More

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December 2018
1 Read

Distinct metabolic adjustments arise from acclimation to constant hypoxia and intermittent hypoxia in estuarine killifish ().

J Exp Biol 2018 Dec 5;221(Pt 23). Epub 2018 Dec 5.

Department of Biology, McMaster University, Hamilton, Ontario, Canada, L8S 4K1.

Many fish experience daily cycles of hypoxia in the wild, but the physiological strategies for coping with intermittent hypoxia are poorly understood. We examined how killifish adjust O supply and demand during acute hypoxia, and how these responses are altered after prolonged acclimation to constant or intermittent patterns of hypoxia exposure. We acclimated killifish to normoxia (∼20 kPa O), constant hypoxia (2 kPa) or intermittent cycles of nocturnal hypoxia (12 h:12 h normoxia:hypoxia) for 28 days, and then compared whole-animal O consumption rates ( ) and tissue metabolites during exposure to 12 h of hypoxia followed by reoxygenation in normoxia. Read More

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December 2018
1 Read

Effects of dietary methionine restriction on postnatal growth, insulin sensitivity, and glucose metabolism in intrauterine growth retardation pigs at 49 and 105 days of age.

J Anim Sci 2018 Dec 2. Epub 2018 Dec 2.

College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.

This study was conducted to investigate the effects of methionine restriction (MR) on growth performance, insulin sensitivity, and hepatic and muscle glucose metabolism in intrauterine growth retardation (IUGR) pigs at 49 and 105 days of age. At weaning (day 21), 30 female normal birth weight (NBW) piglets were fed control diets with adequate methionine (NBW-CON), whereas 60 female IUGR piglets were fed either the control diets (IUGR-CON) or MR diets which were 30% reduced in methionine (IUGR-MR) (n=6 replicates (pens) with 5 piglets/replicate). At 49 and 105 days of age, one pig with a body weight near to the mean of each replication was selected for biochemical analysis. Read More

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December 2018
2 Reads

Real-time simultaneous shim and motion measurement and correction in glycoCEST MRI using double volumetric navigators (DvNavs).

Magn Reson Med 2018 Dec 2. Epub 2018 Dec 2.

UCT Medical Imaging Research Unit, Division of Biomedical Engineering, Department of Human Biology, University of Cape Town, Cape Town, South Africa.

Purpose: CEST MRI allows for indirect detection of molecules with exchangeable protons, measured as a reduction in water signal because of continuous transfer of saturated protons. CEST requires saturation pulses on the order of a second, as well as repeated acquisitions at different offset frequencies. The resulting extended scan time makes CEST susceptible to subject motion, which introduces field inhomogeneity, shifting offset frequencies and causing distortions in CEST spectra that resemble true CEST effects. Read More

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December 2018
1 Read

Label-Free Quantitative Proteomics of Lysine Acetylome Identifies Substrates of Gcn5 in Magnaporthe oryzae Autophagy and Epigenetic Regulation.

mSystems 2018 Nov-Dec;3(6). Epub 2018 Nov 20.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China.

The rice blast fungus Magnaporthe oryzae poses a great threat to global food security. During its conidiation (asexual spore formation) and appressorium (infecting structure) formation, autophagy is induced, serving glycogen breakdown or programmed cell death function, both essential for pathogenicity. Recently, we identified an M. Read More

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November 2018
2 Reads

Data on differentially expressed proteins in retinal emmetropization process in guinea pig using integrated SWATH-based and targeted-based proteomics.

Data Brief 2018 Dec 31;21:1750-1755. Epub 2018 Aug 31.

Laboratory of Experimental Optometry, Centre for Myopia Research, School of Optometry, the Hong Kong Polytechnic University, Kowloon, Hong Kong.

Myopia is generally regarded as a failure of normal emmetropization process, however, its underlying molecular mechanisms are unclear. Retinal protein profile changes using integrated SWATH and MRM-HR MS were studied in guinea pigs at 3- and 21-days of age, where the axial elongation was significantly detected. Differential proteins expressions were identified, and related to pathways which are important in postnatal development in retina, proliferation, breakdown of glycogen-energy and visual phototransduction. Read More

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December 2018
1 Read

Epidermal growth factor-like domain multiple 7 (EGFL7): Expression and possible effect on biliary epithelium growth in cholangiocarcinoma.

Eur J Histochem 2018 Nov 30;62(4). Epub 2018 Nov 30.

Sapienza University of Rome, Department of Anatomical, Histological, Forensic Medicine and Orthopedics Sciences.

Cholangiocarcinoma (CCA) is an aggressive biliary tract malignancy with limited treatment options and low survival rates. The intrahepatic subtype comprises two forms: mucin-iCCA and mixed-iCCA. Epidermal growth factor-like domain multiple (EGFL7) is overexpressed in less differentiated liver tumors. Read More

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November 2018
4 Reads

Oncolytic adenovirus Delta-24-RGD induces a widespread glioma proteotype remodeling during autophagy.

J Proteomics 2018 Nov 29. Epub 2018 Nov 29.

Clinical Neuroproteomics Group, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), Irunlarrea 3, 31008 Pamplona, Spain; IDISNA, Navarra Institute for Health Research, Pamplona, Spain; Proteored-ISCIII, Proteomics Unit, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), Irunlarrea 3, 31008 Pamplona, Spain. Electronic address:

Adenovirus Delta-24-RGD has shown a remarkable efficacy in a phase I clinical trial for glioblastoma. Delta-24-RGD induces autophagy in glioma cells, however, the molecular derangements associated with Delta-24-RGD infection remains poorly understood. Here, proteomics was applied to characterize the glioma metabolic disturbances soon after Delta-24-RGD internalization and late in infection. Read More

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November 2018
1 Read

GSK3β activity alleviates epileptogenesis and limits GluA1 phosphorylation.

EBioMedicine 2018 Nov 27. Epub 2018 Nov 27.

Laboratory of Molecular and Cellular Neurobiology, International Institute of Molecular and Cell Biology, Warsaw 02-109, Poland. Electronic address:

Background: Glycogen synthase kinase-3β (GSK3β) is a key regulator of cellular homeostasis. In neurons, GSK3β contributes to the control of neuronal transmission and plasticity, but its role in epilepsy remains to be defined.

Methods: Biochemical and electrophysiological methods were used to assess the role of GSK3β in regulating neuronal transmission and epileptogenesis. Read More

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November 2018
1 Read

Regulation of glucose uptake and inflammation markers by FOXO1 and FOXO3 in skeletal muscle.

Mol Metab 2018 Nov 16. Epub 2018 Nov 16.

Department of Physiology and Pharmacology, Section for Integrative Physiology, Karolinska Institutet, Stockholm, Sweden; Department of Molecular Medicine and Surgery, Section for Integrative Physiology, Karolinska Institutet, Stockholm, Sweden; Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. Electronic address:

Objective: Forkhead box class O (FOXO) transcription factors regulate whole body energy metabolism, skeletal muscle mass, and substrate switching. FOXO1 and FOXO3 are highly abundant transcription factors, but their precise role in skeletal muscle metabolism has not been fully elucidated.

Methods: To elucidate the role of FOXO in skeletal muscle, dominant negative (dn) constructs for FOXO1 (FOXO1dn) or FOXO3 (FOXO3dn) were transfected by electroporation into mouse tibialis anterior muscle and glucose uptake, signal transduction, and gene expression profiles were assessed after an oral glucose tolerance test. Read More

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November 2018
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Transient receptor potential ion channels mediate adherens junctions dysfunction in a toluene diisocyanate-induced murine asthma model.

Toxicol Sci 2018 Dec 4. Epub 2018 Dec 4.

State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.

Disruption of epithelial cell-cell junctions is essential for the initiation and perpetuation of airway inflammation in asthma. We've previously reported compromised epithelial barrier integrity in a toluene diisocyanate (TDI)-induced occupational asthma model. This study is aimed to explore the role of transient receptor potential vanilloid 4 (TRPV4) and transient receptor potential ankyrin 1 (TRPA1) in the dysfunction of adherens junctions in TDI-induced asthma. Read More

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December 2018
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Diallyl disulphide suppresses the cannonical Wnt signaling pathway and reverses the fibronectin-induced epithelial mesenchymal transition of A549 lung cancer cells.

Food Funct 2018 Dec 5. Epub 2018 Dec 5.

Receptor Biology and Tumor Metastasis, Chittaranjan National Cancer Institute, Kolkata 700026, India.

Globally, non-small cell lung cancer is a leading cause of cancer-related mortality and about 40% of these cancers are detected in the metastatic stage. Epithelial mesenchymal transition (EMT) plays a critical role during malignant transformation, and the extracellular matrix component, fibronectin (FN), is a known inducer of invasion and metastasis. Diallyl disulphide (DADS), a bioactive component of garlic, exhibits a wide spectrum of biological activities including the inhibition of cancer cell migration and invasion. Read More

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December 2018
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