64,746 results match your criteria Glycogen Storage Disease Type IV


Novel metabolic disorders in skeletal muscle of Lipodystrophic Bscl2/Seipin deficient mice.

Mol Cell Endocrinol 2018 Dec 3. Epub 2018 Dec 3.

Department of Physiology, Medical College of Georgia at Augusta University, Augusta, GA, 30912, USA. Electronic address:

Bscl2 mice recapitulate many of the major metabolic manifestations in Berardinelli-Seip congenital lipodystrophy type 2 (BSCL2) individuals, including lipodystrophy, hepatosteatosis, muscular hypertrophy, and insulin resistance. Metabolic defects in Bscl2 mice with regard to glucose and lipid metabolism in skeletal muscle have never been investigated. Here, we identified Bscl2 mice displayed reduced intramyocellular triglyceride (IMTG) content but increased glycogen storage predominantly in oxidative type I soleus muscle (SM). Read More

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December 2018
3 Reads

Differential effects of type 1 diabetes mellitus and subsequent osteoblastic β-catenin activation on trabecular and cortical bone in a mouse model.

Exp Mol Med 2018 Dec 5;50(12):158. Epub 2018 Dec 5.

State Key Laboratory of Trauma, Burn and Combined Injury, Department of War Wound Rescue Skills Training, Base of Army Health Service Training, Army Medical University, 400038, ChongQing, China.

Type 1 diabetes mellitus (T1DM) is a pathological condition associated with osteopenia. WNT/β-catenin signaling is implicated in this process. Trabecular and cortical bone respond differently to WNT/β-catenin signaling in healthy mice. Read More

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December 2018
1 Read

Distinct metabolic adjustments arise from acclimation to constant hypoxia and intermittent hypoxia in estuarine killifish ().

J Exp Biol 2018 Dec 5;221(Pt 23). Epub 2018 Dec 5.

Department of Biology, McMaster University, Hamilton, Ontario, Canada, L8S 4K1.

Many fish experience daily cycles of hypoxia in the wild, but the physiological strategies for coping with intermittent hypoxia are poorly understood. We examined how killifish adjust O supply and demand during acute hypoxia, and how these responses are altered after prolonged acclimation to constant or intermittent patterns of hypoxia exposure. We acclimated killifish to normoxia (∼20 kPa O), constant hypoxia (2 kPa) or intermittent cycles of nocturnal hypoxia (12 h:12 h normoxia:hypoxia) for 28 days, and then compared whole-animal O consumption rates ( ) and tissue metabolites during exposure to 12 h of hypoxia followed by reoxygenation in normoxia. Read More

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December 2018

Effects of dietary methionine restriction on postnatal growth, insulin sensitivity, and glucose metabolism in intrauterine growth retardation pigs at 49 and 105 days of age.

J Anim Sci 2018 Dec 2. Epub 2018 Dec 2.

College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.

This study was conducted to investigate the effects of methionine restriction (MR) on growth performance, insulin sensitivity, and hepatic and muscle glucose metabolism in intrauterine growth retardation (IUGR) pigs at 49 and 105 days of age. At weaning (day 21), 30 female normal birth weight (NBW) piglets were fed control diets with adequate methionine (NBW-CON), whereas 60 female IUGR piglets were fed either the control diets (IUGR-CON) or MR diets which were 30% reduced in methionine (IUGR-MR) (n=6 replicates (pens) with 5 piglets/replicate). At 49 and 105 days of age, one pig with a body weight near to the mean of each replication was selected for biochemical analysis. Read More

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December 2018
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Real-time simultaneous shim and motion measurement and correction in glycoCEST MRI using double volumetric navigators (DvNavs).

Magn Reson Med 2018 Dec 2. Epub 2018 Dec 2.

UCT Medical Imaging Research Unit, Division of Biomedical Engineering, Department of Human Biology, University of Cape Town, Cape Town, South Africa.

Purpose: CEST MRI allows for indirect detection of molecules with exchangeable protons, measured as a reduction in water signal because of continuous transfer of saturated protons. CEST requires saturation pulses on the order of a second, as well as repeated acquisitions at different offset frequencies. The resulting extended scan time makes CEST susceptible to subject motion, which introduces field inhomogeneity, shifting offset frequencies and causing distortions in CEST spectra that resemble true CEST effects. Read More

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December 2018

Label-Free Quantitative Proteomics of Lysine Acetylome Identifies Substrates of Gcn5 in Magnaporthe oryzae Autophagy and Epigenetic Regulation.

mSystems 2018 Nov-Dec;3(6). Epub 2018 Nov 20.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China.

The rice blast fungus Magnaporthe oryzae poses a great threat to global food security. During its conidiation (asexual spore formation) and appressorium (infecting structure) formation, autophagy is induced, serving glycogen breakdown or programmed cell death function, both essential for pathogenicity. Recently, we identified an M. Read More

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November 2018

Data on differentially expressed proteins in retinal emmetropization process in guinea pig using integrated SWATH-based and targeted-based proteomics.

Data Brief 2018 Dec 31;21:1750-1755. Epub 2018 Aug 31.

Laboratory of Experimental Optometry, Centre for Myopia Research, School of Optometry, the Hong Kong Polytechnic University, Kowloon, Hong Kong.

Myopia is generally regarded as a failure of normal emmetropization process, however, its underlying molecular mechanisms are unclear. Retinal protein profile changes using integrated SWATH and MRM-HR MS were studied in guinea pigs at 3- and 21-days of age, where the axial elongation was significantly detected. Differential proteins expressions were identified, and related to pathways which are important in postnatal development in retina, proliferation, breakdown of glycogen-energy and visual phototransduction. Read More

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December 2018

Epidermal growth factor-like domain multiple 7 (EGFL7): Expression and possible effect on biliary epithelium growth in cholangiocarcinoma.

Eur J Histochem 2018 Nov 30;62(4). Epub 2018 Nov 30.

Sapienza University of Rome, Department of Anatomical, Histological, Forensic Medicine and Orthopedics Sciences.

Cholangiocarcinoma (CCA) is an aggressive biliary tract malignancy with limited treatment options and low survival rates. The intrahepatic subtype comprises two forms: mucin-iCCA and mixed-iCCA. Epidermal growth factor-like domain multiple (EGFL7) is overexpressed in less differentiated liver tumors. Read More

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November 2018
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Oncolytic adenovirus Delta-24-RGD induces a widespread glioma proteotype remodeling during autophagy.

J Proteomics 2018 Nov 29. Epub 2018 Nov 29.

Clinical Neuroproteomics Group, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), Irunlarrea 3, 31008 Pamplona, Spain; IDISNA, Navarra Institute for Health Research, Pamplona, Spain; Proteored-ISCIII, Proteomics Unit, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), Irunlarrea 3, 31008 Pamplona, Spain. Electronic address:

Adenovirus Delta-24-RGD has shown a remarkable efficacy in a phase I clinical trial for glioblastoma. Delta-24-RGD induces autophagy in glioma cells, however, the molecular derangements associated with Delta-24-RGD infection remains poorly understood. Here, proteomics was applied to characterize the glioma metabolic disturbances soon after Delta-24-RGD internalization and late in infection. Read More

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November 2018

GSK3β activity alleviates epileptogenesis and limits GluA1 phosphorylation.

EBioMedicine 2018 Nov 27. Epub 2018 Nov 27.

Laboratory of Molecular and Cellular Neurobiology, International Institute of Molecular and Cell Biology, Warsaw 02-109, Poland. Electronic address:

Background: Glycogen synthase kinase-3β (GSK3β) is a key regulator of cellular homeostasis. In neurons, GSK3β contributes to the control of neuronal transmission and plasticity, but its role in epilepsy remains to be defined.

Methods: Biochemical and electrophysiological methods were used to assess the role of GSK3β in regulating neuronal transmission and epileptogenesis. Read More

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November 2018

Regulation of glucose uptake and inflammation markers by FOXO1 and FOXO3 in skeletal muscle.

Mol Metab 2018 Nov 16. Epub 2018 Nov 16.

Department of Physiology and Pharmacology, Section for Integrative Physiology, Karolinska Institutet, Stockholm, Sweden; Department of Molecular Medicine and Surgery, Section for Integrative Physiology, Karolinska Institutet, Stockholm, Sweden; Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. Electronic address:

Objective: Forkhead box class O (FOXO) transcription factors regulate whole body energy metabolism, skeletal muscle mass, and substrate switching. FOXO1 and FOXO3 are highly abundant transcription factors, but their precise role in skeletal muscle metabolism has not been fully elucidated.

Methods: To elucidate the role of FOXO in skeletal muscle, dominant negative (dn) constructs for FOXO1 (FOXO1dn) or FOXO3 (FOXO3dn) were transfected by electroporation into mouse tibialis anterior muscle and glucose uptake, signal transduction, and gene expression profiles were assessed after an oral glucose tolerance test. Read More

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November 2018

Transient receptor potential ion channels mediate adherens junctions dysfunction in a toluene diisocyanate-induced murine asthma model.

Toxicol Sci 2018 Dec 4. Epub 2018 Dec 4.

State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.

Disruption of epithelial cell-cell junctions is essential for the initiation and perpetuation of airway inflammation in asthma. We've previously reported compromised epithelial barrier integrity in a toluene diisocyanate (TDI)-induced occupational asthma model. This study is aimed to explore the role of transient receptor potential vanilloid 4 (TRPV4) and transient receptor potential ankyrin 1 (TRPA1) in the dysfunction of adherens junctions in TDI-induced asthma. Read More

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December 2018
2 Reads

Diallyl disulphide suppresses the cannonical Wnt signaling pathway and reverses the fibronectin-induced epithelial mesenchymal transition of A549 lung cancer cells.

Food Funct 2018 Dec 5. Epub 2018 Dec 5.

Receptor Biology and Tumor Metastasis, Chittaranjan National Cancer Institute, Kolkata 700026, India.

Globally, non-small cell lung cancer is a leading cause of cancer-related mortality and about 40% of these cancers are detected in the metastatic stage. Epithelial mesenchymal transition (EMT) plays a critical role during malignant transformation, and the extracellular matrix component, fibronectin (FN), is a known inducer of invasion and metastasis. Diallyl disulphide (DADS), a bioactive component of garlic, exhibits a wide spectrum of biological activities including the inhibition of cancer cell migration and invasion. Read More

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December 2018

Heart structure in the Amazonian teleost Arapaima gigas (Osteoglossiformes, Arapaimidae).

J Anat 2018 Dec 4. Epub 2018 Dec 4.

Department of Biological Sciences, School of Sciences, São Paulo State University 'Júlio de Mesquita Filho' (UNESP), Bauru, SP, Brazil.

The fish heart ventricle has varied morphology and may have a specific morpho-functional design in species adapted to extreme environmental conditions. In general, the Amazonian ichthyofauna undergoes constant variations in water temperature, pH and oxygen saturation, which makes these species useful for investigations of cardiac morphology. Arapaima gigas, a member of the ancient teleost group Osteoglossomorpha, is one of the largest freshwater fish in the world. Read More

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December 2018

GSK3 suppression upregulates β-catenin and c-Myc to abrogate KRas-dependent tumors.

Nat Commun 2018 Dec 4;9(1):5154. Epub 2018 Dec 4.

Drug Discovery Department, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, 33612, USA.

Mutant KRas is a significant driver of human oncogenesis and confers resistance to therapy, underscoring the need to develop approaches that disable mutant KRas-driven tumors. Because targeting KRas directly has proven difficult, identifying vulnerabilities specific for mutant KRas tumors is an important alternative approach. Here we show that glycogen synthase kinase 3 (GSK3) is required for the in vitro and in vivo growth and survival of human mutant KRas-dependent tumors but is dispensable for mutant KRas-independent tumors. Read More

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December 2018

Tetraspanin 1 promotes epithelial-to-mesenchymal transition and metastasis of cholangiocarcinoma via PI3K/AKT signaling.

J Exp Clin Cancer Res 2018 Dec 4;37(1):300. Epub 2018 Dec 4.

Department of Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Harbin, Heilongjiang, China.

Background: Numerous studies have demonstrated that tetraspanin 1 (TSPAN1), a transmembrane protein, functions as an oncoprotein in many cancer types. However, its role and underlying molecular mechanism in cholangiocarcinoma (CCA) progression remain unclear.

Methods: In the present study, the expression of TSPAN1 in human CCA and adjacent nontumor tissues was examined using real-time PCR, western blot and immunohistochemistry. Read More

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December 2018

Genome-wide characterization and phylogenetic analysis of GSK gene family in three species of cotton: evidence for a role of some GSKs in fiber development and responses to stress.

BMC Plant Biol 2018 Dec 4;18(1):330. Epub 2018 Dec 4.

State Key Laboratory of Cotton Biology, Key Laboratory of Biological and Genetic Breeding of Cotton, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China.

Background: The glycogen synthase kinase 3/shaggy kinase (GSK3) is a serine/threonine kinase with important roles in animals. Although GSK3 genes have been studied for more than 30 years, plant GSK genes have been studied only since the last decade. Previous research has confirmed that plant GSK genes are involved in diverse processes, including floral development, brassinosteroid signaling, and responses to abiotic stresses. Read More

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December 2018

Sublethal Effects of Fruit Extract and Its Pure Glycoalkaloids on the Physiology of (Mealworm).

Toxins (Basel) 2018 Dec 1;10(12). Epub 2018 Dec 1.

Department of Animal Physiology and Development, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University in Poznań, ul. Umultowska 89, 61-614 Poznań, Poland.

Background: plants produce glycoalkaloids (GAs) that affect various physiological processes of herbivorous insects and they are being tested as potential alternatives for synthetic pesticides. They cause lethal and sublethal effects. Nevertheless, their mode of action remains unclear. Read More

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December 2018

Drosophila insulin-like peptide dilp1 increases lifespan and glucagon-like Akh expression epistatic to dilp2.

Aging Cell 2018 Dec 3:e12863. Epub 2018 Dec 3.

Department of Ecology and Evolutionary Biology, Brown University, Providence, Rhode Island.

Insulin/IGF signaling (IIS) regulates essential processes including development, metabolism, and aging. The Drosophila genome encodes eight insulin/IGF-like peptide (dilp) paralogs, including tandem-encoded dilp1 and dilp2. Many reports show that longevity is increased by manipulations that decrease DILP2 levels. Read More

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December 2018
2 Reads

GRK5 influences the phosphorylation of tau via GSK3β and contributes to Alzheimer's disease.

J Cell Physiol 2018 Dec 3. Epub 2018 Dec 3.

Guangdong Key Laboratory of Age-Related Cardiac and Cerebral Diseases, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.

G protein-coupled receptor kinase 5 (GRK5) is a serine/threonine kinase whose dysfunction results in cognitive impairment and Alzheimer-like pathology, including tau hyperphosphorylation. However, the mechanisms whereby GRK5 influences tau phosphorylation remain incompletely understood. In the current study, we showed that GRK5 influenced the phosphorylation of tau via glycogen synthase kinase 3β (GSK3β). Read More

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December 2018
2 Reads

Antifatigue and antiaging effects of Chinese rice wine in mice.

Food Sci Nutr 2018 Nov 18;6(8):2386-2394. Epub 2018 Oct 18.

Key Laboratory of Animal Resistance Biology of Shandong Province School of Life Science Shandong Normal University Jinan China.

Chinese rice wine (CRW) is widely known for keeping good health and commonly used in the traditional Chinese medicine prescription guiding drug. This study assesses the effects of CRW on antifatigue and antiaging activities in mice models. Mice were randomly divided into four groups performing with 0. Read More

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November 2018
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Clinicopathological characteristics of breast sebaceous adenocarcinoma.

Pol J Pathol 2018 ;69(3):226-233

Two middle-aged females presented with a mass located in the lateral quadrant of the breast. Both patients received modified radical surgery for breast cancer, radiotherapy, and chemotherapy and have been living without evidence of disease for more than one year. Under the microscope, we observed that the tumour cells were organised in a solid nest-like or leafy distribution and comprised sebaceous gland cells and oval or fusiform cells. Read More

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January 2018
2 Reads

Asiatic acid protests against myocardial ischemia/reperfusion injury via modulation of glycometabolism in rat cardiomyocyte.

Drug Des Devel Ther 2018 25;12:3573-3582. Epub 2018 Oct 25.

Institute of Translational Medicine, Nanchang University, Nanchang 330031, China,

Background: Asiatic acid is a reported glycogen phosphorylase inhibitor derived from the tropical medicinal plant and exhibits myocardial protection both in vivo and in vitro. The purpose of this study was to evaluate the effects of asiatic acid on myocardial ischemia/reperfusion (MI/R) injury and investigate the underlying mechanisms associated with the modulation of glycometabolism in cardiomyocyte.

Materials And Methods: The rats were subjected to MI/R with or without asiatic acid pretreatment. Read More

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October 2018

Retaining mTeSR1 Medium during Hepatic Differentiation Facilitates Hepatocyte-Like Cell Survival by Decreasing Apoptosis.

Cell Physiol Biochem 2018 Nov 29;51(4):1533-1543. Epub 2018 Nov 29.

Department of Cardiac Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou,

Background/aims: Hepatocyte-like cells derived from human pluripotent stem cells could be an important cell source for hepatocyte transplantation. The present study investigated the effect of retaining mTeSR1 medium during hepatic differentiation on hepatocyte-like cells in vitro.

Methods: Human embryonic stem cell line H1 were treated with activin A and bone morphogenetic protein 4 (BMP4) for definitive endoderm (DE) cell induction and subsequently treated with BMP2 and fibroblast growth factor 4 (FGF4) for early hepatic cell induction. Read More

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November 2018

The flavonoid baicalin improves glucose metabolism by targeting the PH domain of AKT and activating AKT/GSK3β phosphorylation.

FEBS Lett 2018 Nov 29. Epub 2018 Nov 29.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, China.

Baicalin is one of the main flavonoids of the dried root of Scutellaria baicalensis Georgi and is reported to exert beneficial effects on the regulation of glucose/lipid metabolism. However, understanding its specific target and unique mechanism for improving glucose utilization is a challenge. In this paper, target fishing with a baicalin probe reveals that baicalin interacts with AKT. Read More

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November 2018
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No evidence of direct association between GLUT4 and glycogen in human skeletal muscle.

Physiol Rep 2018 Nov;6(22):e13917

Department of Physiology, The University of Melbourne, Melbourne, Australia.

Previous studies have demonstrated that exercise increases whole body and skeletal muscle insulin sensitivity that is linked with increased GLUT4 at the plasma membrane following insulin stimulation and associated with muscle glycogen depletion. To assess the potential direct association between muscle glycogen and GLUT4, seven untrained, male subjects exercised for 60 min at ~75% VO peak, with muscle samples obtained by percutaneous needle biopsy immediately before and after exercise. Exercise reduced muscle glycogen content by ~43%. Read More

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November 2018
2 Reads

Community-Level Physiological Profiling for Microbial Community Function in Broiler Ceca.

Curr Microbiol 2018 Nov 28. Epub 2018 Nov 28.

Poultry Microbiological Safety and Processing Research Unit, U.S. National Poultry Research Center, Agricultural Research Service, U.S. Department of Agriculture, 950 College Station Road, Athens, GA, 30605-2720, USA.

Poultry production is a major agricultural output worldwide. It is known that the gut health of broilers is essential for their growth and for providing wholesome products for human consumption. Previously, the microbial diversity of broiler ceca was studied at the genetic level. Read More

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November 2018
1 Read

Anti-fatigue and anti-oxidant activities of oyster (Ostrea rivularis) hydrolysate prepared by compound protease.

Food Funct 2018 Nov 29. Epub 2018 Nov 29.

College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, People's Republic of China.

Oyster, which is rich in protein and widely used as a marine traditional Chinese medicine, was believed to have good curative effects in health care and on chronic diseases. This study was designed to evaluate the anti-fatigue and anti-oxidant effects of oyster hydrolysate. Oyster meat (OM) was hydrolyzed with a complex protease, and oyster hydrolysate (OH) was separated by a 6 kDa ultrafiltration membrane into two fractions, OH-I (<6 kDa) and OH-II (≥6 kDa). Read More

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November 2018

Blood-Based Kinase Assessments in Alzheimer's Disease.

Front Aging Neurosci 2018 14;10:338. Epub 2018 Nov 14.

Center of Cognitive Neurology, Lariboisiere Fernand-Widal Hospital, APHP, University Paris Diderot, Paris, France.

Alzheimer's disease (AD) is marked by memory disturbances followed by aphasia, apraxia and agnosia. Brain lesions include the accumulation of the amyloid peptide in extracellular plaques, neurofibrillary tangles with abnormally phosphorylated tau protein and synaptic and neuronal loss. New findings have suggested that brain lesions could occur one or two decades before the first clinical signs. Read More

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November 2018

[Differentiated hypoglycemic effects of baicalin, berberine and puerarin on insulin-resistance HepG2 cells].

Zhongguo Zhong Yao Za Zhi 2018 Oct;43(20):4097-4103

School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.

To investigate the hypoglycemic effects of baicalin, berberine, puerarin and liquiritin on the insulin resistance (IR) cells. The IR model of HepG2 cells was established by treatment with insulin and dexamethasone for 48 h. Glucose uptake, glycogen content and cell viability were detected with different concentrations of baicalin, berberine, puerarin, liquiritin in IR-HepG2 cells. Read More

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October 2018

An Improved Genome-Scale Metabolic Model of C1 (AK888) and Its Application in Glycogen Overproduction.

Metabolites 2018 Nov 26;8(4). Epub 2018 Nov 26.

Department of Chemical Engineering, Faculty of Engineering, King Mongkut's University of Technology Thonburi, Bangkok 10140, Thailand.

Glycogen-enriched biomass of has increasingly gained attention as a source for bioethanol production. To study the metabolic capabilities of glycogen production in C1, a genome-scale metabolic model (GEM) could be a useful tool for predicting cellular behavior and suggesting strategies for glycogen overproduction. New experimentally validated GEM of C1 namely AK888, which has improved metabolic coverage and functionality was employed in this research. Read More

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November 2018

[Genes and molecular mechanisms affecting the correlation between liver cancer and diabetes mellitus].

Zhonghua Gan Zang Bing Za Zhi 2018 Sep;26(9):718-720

Key Laboratory of Hepatobiliary Surgery and Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.

Hepatocellular carcinoma (HCC) is a common and high incidence disease in China. It is presently thought that diabetes is one of the independent risk factor for HCC. Diabetes and liver cancer are closely related, but the relationship and mechanism of diabetes and liver cancer are quite complex and controversial. Read More

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September 2018
1 Read

Cellular physiology controls photoautotrophic production of 1,2-propanediol from pools of CO and glycogen.

Biotechnol Bioeng 2018 Nov 27. Epub 2018 Nov 27.

Department Solar Materials, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany.

Synechocystis sp. PCC 6803 PG is a cyanobacterial strain capable of synthesizing 1,2-propanediol from carbon dioxide via a heterologous three-step pathway and a methylglyoxal synthase originating from E. coli as initial enzyme. Read More

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November 2018

Effect of diets supplemented with linseed alone or combined with vitamin E and selenium or with plant extracts, on transcriptome in growing-finishing Italian Large White pigs.

J Anim Sci Biotechnol 2018 20;9:81. Epub 2018 Nov 20.

1Interdepartmental Centre for Industrial Agrifood Research (CIRI- AGRO), University of Bologna, Via Quinto Bucci 336, I-47521 Cesena, Italy.

Background: Supplementing farm animals diet with functional ingredients may improve the nutritional quality of meat products. Diet composition has been also demonstrated to influence the gene expression with effect on biological processes and pathways. However, the knowledge on the effect of nutrients at the molecular level is scant. Read More

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November 2018
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Polyphosphate-accumulating organisms in full-scale tropical wastewater treatment plants use diverse carbon sources.

Water Res 2018 Nov 9;149:496-510. Epub 2018 Nov 9.

Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, 637551, Singapore; Department of Civil and Environmental Engineering, One Shields Avenue, University of California, Davis, CA, 95616, USA; School of Civil and Environmental Engineering, Nanyang Technological University, Singapore, 639798, Singapore. Electronic address:

Enhanced biological phosphorus removal (EBPR) is considered challenging in the tropics, based on a great number of laboratory-based studies showing that the polyphosphate-accumulating organism (PAO) Candidatus Accumulibacter does not compete well with glycogen accumulating organisms (GAOs) at temperatures above 25 °C. Yet limited information is available on the PAO community and the metabolic capabilities in full-scale EBPR systems operating at high temperature. We studied the composition of the key functional PAO communities in three full-scale wastewater treatment plants (WWTPs) with high in-situ EBPR activity in Singapore, their EBPR-associated carbon usage characteristics, and the relationship between carbon usage and community composition. Read More

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November 2018

Genomic insights into the metabolism of 'Candidatus Defluviicoccus seviourii', a member of Defluviicoccus cluster III abundant in industrial activated sludge.

FEMS Microbiol Ecol 2018 Nov 26. Epub 2018 Nov 26.

Department of Wine & Food Science, University of Adelaide.

Filamentous cluster III Defluviicoccus (DF3) are known to proliferate and cause bulking issues in industrial wastewater treatment plants. Members of the genus Defluviicoccus are also known to exhibit the glycogen accumulating organism (GAO) phenotype, which is suggested to be detrimental to enhanced biological phosphorus removal (EBPR). Despite the reported negative impact members of the DF3 have on activated sludge wastewater treatment systems, limited research has focused on understanding the physiological traits that allow them to compete in these environments. Read More

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November 2018
2 Reads

Device-based treatment for vaginal wellness.

Semin Cutan Med Surg 2018 Dec;37(4):226-232

Yale University School of Medicine, New Haven, Connecticut, USA.

Genitourinary syndrome of menopause (GSM), encompassing the disorders of atrophic vaginitis, urinary incontinence, and pelvic prolapse, affects the majority of postmenopausal women, as well as patients who are undergoing breast cancer treatement, post-ovarectomy, post-radiation, and breast-feeding. There is a need for better treatment options for these common conditions that adversely affect physical function and quality of life and that are often underserved by existing options. Lasers have been used to treat genitourinary tissue for over 40 years, and over the past decade, several lasers and radiofrequency devices have been developed and clinically tested for the treatment of GSM, with an accumulating body of evidence demonstrating their safety and efficacy. Read More

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December 2018
3 Reads

The neuroprotective effect of agmatine against amyloid β-induced apoptosis in primary cultured hippocampal cells involving ERK, Akt/GSK-3β, and TNF-α.

Mol Biol Rep 2018 Nov 24. Epub 2018 Nov 24.

Shiraz Nuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

β-Amyloid peptide (Aβ), the major element of senile plaques in Alzheimer's disease (AD), has been found to accumulate in brain regions critical for memory and cognition. Deposits of Aβ trigger neurotoxic events which lead to neural apoptotic death. The present study examined whether agmatine, an endogenous polyamine formed by the decarboxylation of L-arginine, possesses a neuroprotective effect against Aβ-induced toxicity. Read More

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November 2018
1 Read

Ameliorative potential of Cortex Lycii on enzymes involved in carbohydrate metabolism in streptozotocin-nicotinamide induced diabetic rats.

Pak J Pharm Sci 2018 Nov;31(6):2419-2428

Dongtai Institute of Tidal Flat, Nanjing Branch of Chinese Academy of Sciences, Dongtai, China.

Cortex Lycii (root back of Lycium chinense) has is a famous traditional Chinese medicine which displays several pharmacological activities including antioxidant and antidiabetic properties. We investigated the effect of the ethyl acetate fraction (QCL) of Cortex Lycii on the enzymes involved in the metabolism of carbohydrate in diabetic rat models. Streptozotocin-nicotinamide (110 and 65mg/kg body weight, respectively) was used to induce diabetes. Read More

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November 2018
5 Reads

Continuous zebularine treatment enhances hepatic differentiation of mesenchymal stem cells under liver-specific factors induction in vitro.

Life Sci 2018 Dec 25;215:57-63. Epub 2018 Oct 25.

Department of Radiology, The Second Xiangya Hospital, Central-South University, Changsha, Hunan 410011, China. Electronic address:

Aims: To investigate the effect of zebularine, a stable inhibitor of DNA methylation, on hepatic differentiation of bone marrow-derived mesenchymal stem cells (BM-MSCs) under liver-specific factors induction in vitro.

Main Methods: BM-MSCs were isolated from the mononuclear cell fraction of rabbit bone marrow samples. The identification of these cells was carried out by immunophenotype analysis. Read More

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December 2018

The aromatic amino acid sensor GPR142 controls metabolism through balanced regulation of pancreatic and gut hormones.

Mol Metab 2018 Nov 5. Epub 2018 Nov 5.

Section for Metabolic Receptology, Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark; Laboratory for Molecular Pharmacology, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. Electronic address:

Objectives: GPR142, which is highly expressed in pancreatic islets, has recently been deorphanized as a receptor for aromatic amino acids; however, its physiological role and pharmacological potential is unclear.

Methods And Results: We find that GPR142 is expressed not only in β- but also in α-cells of the islets as well as in enteroendocrine cells, and we confirm that GPR142 is a highly selective sensor of essential aromatic amino acids, in particular Trp and oligopeptides with N-terminal Trp. GPR142 knock-out mice displayed a very limited metabolic phenotype but demonstrated that L-Trp induced secretion of pancreatic and gut hormones is mediated through GPR142 but that the receptor is not required for protein-induced hormone secretion. Read More

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November 2018
3 Reads

Neuroinflammation and aberrant hippocampal plasticity in a mouse model of emotional stress evoked by exposure to ultrasound of alternating frequencies.

Prog Neuropsychopharmacol Biol Psychiatry 2018 Nov 22;90:104-116. Epub 2018 Nov 22.

Department of Neuroscience, Maastricht University, Universiteitssingel 40, NL 6229ER, Maastricht, Netherlands; Institute of General Pathology and Pathophysiology, Baltiiskaya str, 8, Moscow 125315, Russia; Sechenov First Moscow State Medical University, Institute of Molecular Medicine, Laboratory of Psychiatric Neurobiology and Department of Normal Physiology, Trubetskaya street 8-2, 119991, Moscow, Russia. Electronic address:

Emotional stress is a form of stress evoked by processing negative mental experience rather than an organic or physical disturbance and is a frequent cause of neuropsychiatric pathologies, including depression. Susceptibility to emotional stress is commonly regarded as a human-specific trait that is challenging to model in other species. Recently, we showed that a 3-week-long exposure to ultrasound of unpredictable alternating frequencies within the ranges of 20-25 kHz and 25-45 kHz can induce depression-like characteristics in laboratory mice and rats. Read More

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November 2018
4 Reads

Probiotic Lactobacillus gasseri SBT2055 improves insulin secretion in a diabetic rat model.

J Dairy Sci 2018 Nov 21. Epub 2018 Nov 21.

Laboratory of Nutrition Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. Electronic address:

The probiotic Lactobacillus gasseri SBT2055 (LG2055) has a protective effect against metabolic syndrome in rats and humans. Metabolic syndrome increases the risk of type 2 diabetes mellitus. In this study, Goto-Kakizaki rats were used as a diabetic model and fed diets containing LG2055-fermented or nonfermented skim milk for 4 wk. Read More

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November 2018
5 Reads

Hypoglycemic Effects of Glimepiride in Sulfonylurea Receptor Deficient Rat.

Br J Pharmacol 2018 Nov 24. Epub 2018 Nov 24.

Department of Endocrinology, Shandong Provincial Qianfoshan Hospital, Shandong University, Shandong, China.

Background And Purpose: Sulfonylureas (SUs) were suggested to have an insulin-independent blood glucose decreasing activity due to extrapancreatic effect, however, lack of adequate in vivo evidences makes this statement controversial. Here we aimed to evaluate whether glimepiride exists extrapancreatic blood glucose-lowering activity in vivo.

Experimental Approach: Sulfonylurea receptor deficient (SUR1 ) rat was created by TALEN technique. Read More

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November 2018
1 Read

Next-generation glycogen storage diseases.

J Inherit Metab Dis 2018 Nov 23. Epub 2018 Nov 23.

Medical Genetics Service, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.

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November 2018

Behavioural and physiological responses to low- and high-intensity locomotion in Chinese shrimp Fenneropenaeus chinensis.

J Comp Physiol A Neuroethol Sens Neural Behav Physiol 2018 Nov 23. Epub 2018 Nov 23.

The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, China.

We explored stroke behaviour, energy sources, and their related metabolic enzymes during multi-intensity swimming and tail-flipping at low- and high-intensity modes in Chinese shrimp Fenneropenaeus chinensis. In swimming, shrimp were encouraged to swim at velocities of 3, 6, 9 cm s for 200 min (low-intensity), and at 12, 15, 18 cm s until fatigue (high-intensity). In tail-flipping, shrimp were encouraged to tail-flip by tapping cephalothorax at frequencies of 0. Read More

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November 2018
2 Reads

Leea macrophylla root extract upregulates the mRNA expression for antioxidative enzymes and repairs the necrosis of pancreatic β-cell and kidney tissues in fructose-fed Type 2 diabetic rats.

Biomed Pharmacother 2018 Nov 19;110:74-84. Epub 2018 Nov 19.

Designated Reference Institute for Chemical Measurements (DRiCM), Bangladesh Council of Scientific & Industrial Research (BCSIR), Dr. Qudrat-i-Khuda Road, Dhanmondi, Dhaka, 1205, Bangladesh.

This research investigated the functional food effect of Leea macrophylla (Roxb.) ex Hornem root extract on pancreatic necrosis in Streptozotocin-induced type-2 diabetes. Prior to animal intervention, Leea macrophylla root extract (LMR) was subjected to GC-MS analysis. Read More

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November 2018
4 Reads