3,267 results match your criteria Glycobiology [Journal]


POLYSIALIC ACID IS EXPRESSED IN HUMAN NAÏVE CD4+ T CELLS AND IS INVOLVED IN MODULATING ACTIVATION.

Glycobiology 2019 Apr 16. Epub 2019 Apr 16.

Departamento de Biología, Universidad de Guanajuato, Guanajuato, Gto 36050 México.

The activation of human naïve CD4+ T cells, responsible for orchestrating the immune response, has been reported to cause increased de novo sialylation and overexpression of the genes coding for polysialyltransferases ST8Sia II and ST8Sia IV, suggesting the potential of CD4+ T cells to synthesize polysialic acid (PSA), a type of glycosylation not previously described in these cells. PSA has been found as a post-translational modification in a limited number of mammalian proteins, having a very relevant role in modulating interactions due to its characteristic biophysical properties. In this work, we confirm that human CD4+ T cells express both polysialyltransferases and synthesize PSA, as assessed with the anti-PSA monoclonal antibody (mAb) 12E3. Read More

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https://academic.oup.com/glycob/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/glycob/cwz032DOI Listing
April 2019
1 Read

Glycoengineering bioconjugate vaccines, therapeutics, and diagnostics in E. coli.

Glycobiology 2019 Apr 16. Epub 2019 Apr 16.

VaxNewMo, St. Louis, MO, USA.

The first, general glycosylation pathway in bacteria, the N-linked glycosylation system of Campylobacter jejuni, was discovered two decades ago. Since then, many diverse prokaryotic glycosylation systems have been characterized, including O-linked glycosylation systems that have no homologous counterparts in eukaryotic organisms. Shortly after these discoveries, glycosylation pathways were recombinantly introduced into E. Read More

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http://dx.doi.org/10.1093/glycob/cwz031DOI Listing

Characterising the selectivity of ER α-glucosidase inhibitors.

Glycobiology 2019 Apr 12. Epub 2019 Apr 12.

School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United Kingdom.

The endoplasmic reticulum (ER) contains both α-glucosidases and α-mannosidases which process the N-linked oligosaccharides of newly synthesised glycoproteins and thereby facilitate polypeptide folding and glycoprotein quality control. By acting as structural mimetics, iminosugars can selectively inhibit these ER localised α-glycosidases, preventing N-glycan trimming and providing a molecular basis for their therapeutic applications. In this study, we investigate the effects of a panel of nine iminosugars on the actions of ER luminal α-glucosidase I and α-glucosidase II. Read More

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http://dx.doi.org/10.1093/glycob/cwz029DOI Listing

Directed evolution of bacterial polysialyltransferases.

Glycobiology 2019 Apr 12. Epub 2019 Apr 12.

Department of Chemistry and Biology, Ryerson University, Toronto, ON, Canada.

Polysialyltransferases (polySTs) are glycosyltransferases that synthesize polymers of sialic acid found in vertebrates and some bacterial pathogens. Bacterial polySTs have utility in the modification of therapeutic proteins to improve serum half-life, and the potential for tissue engineering. PolySTs are membrane-associated proteins and as recombinant proteins suffer from inherently low solubility, low expression levels and poor thermal stability. Read More

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https://academic.oup.com/glycob/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/glycob/cwz021DOI Listing
April 2019
1 Read

Structures and chitin-binding properties of two N-terminal lysin motifs (LysMs) found in a chitinase from Volvox carteri.

Glycobiology 2019 Apr 12. Epub 2019 Apr 12.

Department of Advanced Bioscience, Kindai University, 3327-204 Nakamachi, Nara Japan.

Two N-terminal lysin motifs (LysMs) found in a chitinase from the green alga Volvox carteri (VcLysM1 and VcLysM2) were produced, and their structures and chitin-binding properties were characterized. The binding affinities of VcLysM1 toward chitin oligomers determined by isothermal titration calorimetry (ITC) were higher than those of VcLysM2 by 0.8-1. Read More

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http://dx.doi.org/10.1093/glycob/cwz024DOI Listing

Sialic acid glycoengineering using N-acetylmannosamine and sialic acid analogues.

Glycobiology 2019 Mar 26. Epub 2019 Mar 26.

Radiotherapy & OncoImmunology Laboratory, Department of Radiation Oncology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Geert Grooteplein Zuid 32, Nijmegen, The Netherlands.

Sialic acids cap the glycans of cell surface glycoproteins and glycolipids. They are involved in a multitude of biological processes and aberrant sialic acid expression is associated with several pathologies. Sialic acids modulate the characteristics and functions of glycoproteins and regulate cell-cell as well as cell-extracellular matrix interactions. Read More

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http://dx.doi.org/10.1093/glycob/cwz026DOI Listing

GRITS Toolbox - a freely available software for processing, annotating, and archiving glycomics mass spectrometry data.

Glycobiology 2019 Mar 26. Epub 2019 Mar 26.

Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA.

Mass spectrometry (MS) is one of the most effective techniques for high-throughput, high-resolution characterization of glycan structures. Although many software applications have been developed over the last decades for the interpretation of MS data of glycan structures, only a few are capable of dealing with the large data sets produced by glycomics analysis. Furthermore, these applications utilize databases that can lead to redundant glycan annotations and do not support post-processing of the data within the software or by third party applications. Read More

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https://academic.oup.com/glycob/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/glycob/cwz023DOI Listing
March 2019
3 Reads

Structural basis for specific recognition of core fucosylation in N-glycans by Pholiota squarrosa lectin (PhoSL).

Glycobiology 2019 Mar 26. Epub 2019 Mar 26.

Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.

Fucosylation of the N-glycan core via the α1-6 linkage (core fucosylation) is detected in specific types of cancers and related diseases, and thereby serves for a relevant biomarker. The lectin from a mushroom Pholiota squarrosa (PhoSL) shows a clear specificity to core fucosylation, without recognizing those with other types of fucosylation, such as the H type via the α1-2 linkage or the Lewis type via the α1-3 or α1-4 linkage. Here we determined the crystal structure of the PhoSL trimer in complex with a disaccharide fucose(α1-6)N-acetylglucosamine (GlcNAc). Read More

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https://academic.oup.com/glycob/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/glycob/cwz025DOI Listing
March 2019
2 Reads

Galectin-2 suppresses nematode development by binding to the invertebrate-specific galactoseβ1-4fucose glyco-epitope.

Glycobiology 2019 Mar 15. Epub 2019 Mar 15.

Josai University, Faculty of Pharmacy and Pharmaceutical Sciences, 1-1 Keyakidai, Sakado, Saitama, Japan.

Galactoseβ1-4Fucose (GalFuc) is a unique disaccharide found in invertebrates including nematodes. A fungal galectin CGL2 suppresses nematode development by recognizing the galactoseβ1-4fucose epitope. The Caenorhabditis elegans galectin LEC-6 recognizes it as an endogenous ligand and the Glu67 residue of LEC-6 is responsible for this interaction. Read More

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http://dx.doi.org/10.1093/glycob/cwz022DOI Listing

Human Adenovirus type 5 increases host cell fucosylation and modifies Ley antigen expression.

Glycobiology 2019 Mar 14. Epub 2019 Mar 14.

Laboratorio de Glicobiología Humana y Diagnóstico Molecular; Centro de Investigación en Dinámica Celular, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos. Av. Universidad 1001, Cuernavaca, México.

Certain viral infections are known to modify the glycosylation profile of infected cells through the overexpression of specific host cell fucosyltransferases (FUTs). Infection with CMV (cytomegalovirus), HCV (hepatitis C virus), HSV-1 (herpes simplex virus type-1) and VZV (varicella-zoster virus) increase the expression of fucosylated epitopes, including antigens sLex (Siaα2-3 Galβ1-4(Fucα1-3)GlcNAcβ1-R) and Ley (Fucα1-2 Galβ1-4(Fucα1-3)GlcNAcβ1-R). The reorganization of the glycocalyx induced by viral infection may favor the spread of viral progeny, and alter diverse biological functions mediated by glycans, including recognition by the adaptive immune system. Read More

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https://academic.oup.com/glycob/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/glycob/cwz017DOI Listing
March 2019
5 Reads

Recombinant dermatan sulfate is a potent activator of heparin cofactor II-dependent inhibition of thrombin.

Glycobiology 2019 Mar 14. Epub 2019 Mar 14.

Department of Experimental Medical Science, Lund University, Sweden.

The glycosaminoglycan dermatan sulfate (DS) is a well-known activator of heparin cofactor II-dependent inactivation of thrombin. In contrast to heparin, dermatan sulfate has never been prepared recombinantly from material of non-animal origin. Here we report on the enzymatic synthesis of structurally well-defined DS with high anticoagulant activity. Read More

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http://dx.doi.org/10.1093/glycob/cwz019DOI Listing
March 2019
1 Read
3.147 Impact Factor

Establishment and characterization of Drosophila cell lines mutant for heparan sulfate modifying enzymes.

Glycobiology 2019 Mar 14. Epub 2019 Mar 14.

From the Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA.

A class of carbohydrate-modified proteins, heparan sulfate proteoglycans (HSPGs), play critical roles both in normal development and during disease. Genetic studies using a model organism, Drosophila, have been contributing to understanding the in vivo functions of HSPGs. Despite the many strengths of the Drosophila model for in vivo studies, biochemical analysis of Drosophila HS is somewhat limited, mainly due to the insufficient amount of the material obtained from the animal. Read More

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http://dx.doi.org/10.1093/glycob/cwz020DOI Listing

ProGlycProt V2.0, a repository of experimentally validated glycoproteins and protein glycosyltransferases of prokaryotes.

Glycobiology 2019 Mar 5. Epub 2019 Mar 5.

CSIR-Institute of Microbial Technology, Sector 39 A, Chandigarh, India.

Knowledge of glycosylation status and glycan-pattern of proteins are of considerable medical, academic and application interest. ProGlycProt V2.0 (www. Read More

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http://dx.doi.org/10.1093/glycob/cwz013DOI Listing

An updated view of the oligosaccharyltransferase complex in Plasmodium.

Glycobiology 2019 Mar 5. Epub 2019 Mar 5.

Bioinformatics Research Laboratory, Department of Biological Sciences, University of Cyprus, Nicosia, Cyprus.

Despite the controversy regarding the importance of protein N-linked glycosylation in species of the genus Plasmodium, genes potentially encoding core subunits of the oligosaccharyltransferase (OST) complex have already been characterized in completely sequenced genomes of malaria parasites. Nevertheless, the currently established notion is that only four out of eight subunits of the OST complex-which is considered conserved across eukaryotes-are present in Plasmodium species. In this study, we carefully conduct computational analysis to provide unequivocal evidence that all components of the OST complex, with the exception of Swp1/Ribophorin II, can be reliably identified within completely sequenced plasmodial genomes. Read More

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https://academic.oup.com/glycob/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/glycob/cwz011DOI Listing
March 2019
4 Reads

Structure of the zebrafish galectin-1-L2 and model of its interaction with the infectious hematopoietic necrosis virus (IHNV) envelope glycoprotein.

Glycobiology 2019 Mar 5. Epub 2019 Mar 5.

Department of Neurology and Johns Hopkins University School of Medicine, Baltimore, MD.

Galectins, highly conserved β-galactoside-binding lectins, have diverse regulatory roles in development and immune homeostasis and can mediate protective functions during microbial infection. In recent years, the role of galectins in viral infection has generated considerable interest. Studies on highly pathogenic viruses have provided invaluable insight into the participation of galectins in various stages of viral infection, including attachment and entry. Read More

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http://dx.doi.org/10.1093/glycob/cwz015DOI Listing
March 2019
4 Reads

Prevalence of auto-antibodies against D-ribose-glycated-hemoglobin in diabetes mellitus.

Glycobiology 2019 Mar 5. Epub 2019 Mar 5.

Department of Biosciences, Integral University, Lucknow, India.

Glycation of biological macromolecules, due to hyperglycemia, promotes the formation of advanced glycation end products (AGEs). It is accelerated in diabetic patients and is responsible for the pathophysiology and progression of diabetes. Previous reports have shown that amount of AGEs formation and glycation-induced structural damage is higher in hemoglobin (Hb) than other proteins present in blood. Read More

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http://dx.doi.org/10.1093/glycob/cwz012DOI Listing

Functional analyses of the UDP-galactose transporter SLC35A2 using the binding of bacterial Shiga toxins as a novel activity assay.

Glycobiology 2019 Mar 5. Epub 2019 Mar 5.

Division of Pharmacology & Toxicology, College of Pharmacy, Institute for Cellular & Molecular Biology, and Institute for Neuroscience, The University of Texas at Austin, Austin, TX.

SLC35A2 transports UDP-galactose from the cytosol to the lumen of the Golgi apparatus and endoplasmic reticulum for glycosylation. Mutations in SLC35A2 induce a congenital disorder of glycosylation. Despite the biomedical relevance, mechanisms of transport via SLC35A2 and the impact of disease-associated mutations on activity are unclear. Read More

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http://dx.doi.org/10.1093/glycob/cwz016DOI Listing

The influence of heteromultivalency on lectin-glycan binding behavior.

Glycobiology 2019 Mar 2. Epub 2019 Mar 2.

Department of Chemical Engineering, Texas A&M University, College Station, TX, USA.

We recently discovered that the nature of lectin multivalency and glycolipid diffusion on cell membranes could lead to the heteromultivalent binding (i.e., a single lectin simultaneously binding to different types of glycolipid ligands). Read More

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http://dx.doi.org/10.1093/glycob/cwz010DOI Listing
March 2019
3 Reads

Identification of a non-canonical chondroitin sulfate linkage region trisaccharide.

Glycobiology 2019 Mar 2. Epub 2019 Mar 2.

Department of Laboratory Medicine, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden.

It is generally accepted that the biosynthesis of chondroitin sulfate and heparan sulfate is proceeding from a common linkage region tetrasaccharide comprising GlcA-Gal-Gal-Xyl-O-. The linkage region can undergo various modifications such as sulfation, phosphorylation, and sialylation, and as the methods for studying glycosaminoglycan structure have been developed and refined, the number of discovered modifications has increased. Previous studies on the linkage region and the glycosyltransferases involved in the biosynthesis suggest that variants of the linkage region tetrasaccharide may also be possible. Read More

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http://dx.doi.org/10.1093/glycob/cwz014DOI Listing

The Minimum Information Required for a Glycomics Experiment (MIRAGE) Project: LC Guidelines.

Glycobiology 2019 Feb 19. Epub 2019 Feb 19.

Beilstein-Institut, Trakehner Str. 7-9, Frankfurt am Main, Germany.

The Minimum Information Required for A Glycomics Experiment (MIRAGE) is an initiative created by experts in the fields of glycobiology, glycoanalytics and glycoinformatics to design guidelines that improve the reporting and reproducibility of glycoanalytical methods. Previously, the MIRAGE Commission has published guidelines for describing sample preparation methods and the reporting of glycan array and mass spectrometry techniques and data collections. Here, we present the first version of guidelines that aim to improve the quality of the reporting of liquid chromatography (LC) glycan data in the scientific literature. Read More

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http://dx.doi.org/10.1093/glycob/cwz009DOI Listing
February 2019

Expanding CSDB_GT glycosyltransferase database with Escherichia coli.

Glycobiology 2019 04;29(4):285-287

N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, Moscow, Russia.

In 2017, we reported a new database on glycosyltransferase (GT) activities, CSDB_GT (http://csdb.glycoscience.ru/gt. Read More

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http://dx.doi.org/10.1093/glycob/cwz006DOI Listing

IgA1 hinge-region clustered glycan fidelity is established early during semi-ordered glycosylation by GalNAc-T2.

Glycobiology 2019 Jan 31. Epub 2019 Jan 31.

Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL.

GalNAc-type O-glycans are often added to proteins post-translationally in a clustered manner in repeat regions of proteins, such as mucins and IgA1. Observed IgA1 glycosylation patterns show that glycans occur at similar sites with similar structures. It is not clear how the sites and number of glycans added to IgA1, or other proteins, can follow a conservative process. Read More

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http://dx.doi.org/10.1093/glycob/cwz007DOI Listing
January 2019
3 Reads

A strategy for generating cancer-specific monoclonal antibodies to aberrant O-glycoproteins: identification of a novel dysadherin-Tn antibody.

Glycobiology 2019 04;29(4):307-319

Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, Copenhagen N, Denmark.

Successful application of potent antibody-based T-cell engaging immunotherapeutic strategies is currently limited mainly to hematological cancers. One major reason is the lack of well-characterized antigens on solid tumors with sufficient cancer specific expression. Aberrantly O-glycosylated proteins contain promising cancer-specific O-glycopeptide epitopes suitable for immunotherapeutic applications, but currently only few examples of such antibody epitopes have been identified. Read More

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http://dx.doi.org/10.1093/glycob/cwz004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6430981PMC
April 2019
1 Read

Mass spectrometry analysis reveals aberrant N-glycans in colorectal cancer tissues.

Glycobiology 2019 Jan 30. Epub 2019 Jan 30.

Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Science, Soochow University, 199 Ren-Ai Road, SuZhou, China.

Aberrant glycosylation is strongly correlated with the development of various cancers. Tumor-associated carbohydrate antigens, including N-glycans, are predominantly expressed on the tumor cell surface. Because the incidence of colorectal cancer is high in China, we investigated aberrant N-glycans from colorectal cancer tissues (CRC) in Chinese patients. Read More

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https://academic.oup.com/glycob/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/glycob/cwz005DOI Listing
January 2019
7 Reads

Hyaluronan histochemistry-a potential new tool to assess the progress of liver disease from simple steatosis to hepatocellular carcinoma.

Glycobiology 2019 04;29(4):298-306

School of Medicine, Institute of Biomedicine, Faculty of Health Sciences, University of Eastern Finland, Kuopio, Finland.

Nonalcoholic fatty liver disease is among the most common liver diseases worldwide and one cause of cirrhosis that can result in the development of hepatocellular carcinoma (HCC). Hyaluronan (HA) is a high-molecular-mass glycosaminoglycan with diverse functions in tissue injury and repair, for instance, in inflammation and fibrogenesis. The aim of the present study was to investigate the relationships between the HA synthesizing and degrading enzymes in a spectrum of liver pathologies. Read More

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http://dx.doi.org/10.1093/glycob/cwz002DOI Listing

Lectins as possible tools for improved urinary bladder cancer management.

Glycobiology 2019 Jan 25. Epub 2019 Jan 25.

Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.

Urinary bladder cancer is the ninth most common cancer in developed countries with poor prognosis and outcome for the patient due to the challenging diagnosis and limited treatment possibilities. Bladder cancer arises mainly from urothelial cells lining the lumen. Urothelial cells form a three- to five-layered urothelium, which maintains the blood-urine barrier. Read More

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http://dx.doi.org/10.1093/glycob/cwz001DOI Listing
January 2019
1 Read

CHARMM-GUI Glycan Modeler for modeling and simulation of carbohydrates and glycoconjugates.

Glycobiology 2019 04;29(4):320-331

Departments of Biological Sciences and Bioengineering, Lehigh University, Bethlehem, PA, USA.

Characterizing glycans and glycoconjugates in the context of three-dimensional structures is important in understanding their biological roles and developing efficient therapeutic agents. Computational modeling and molecular simulation have become an essential tool complementary to experimental methods. Here, we present a computational tool, Glycan Modeler for in silico N-/O-glycosylation of the target protein and generation of carbohydrate-only systems. Read More

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https://academic.oup.com/glycob/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/glycob/cwz003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6422236PMC
April 2019
8 Reads

Changes in the physico-chemical properties of the xanthan produced by Xanthomonas citri subsp. citri in grapefruit leaf extract.

Glycobiology 2019 03;29(3):269-278

Instituto de Ciencia y Tecnología Dr. César Milstein, Fundación Pablo Cassará, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Saladillo 2468 (C1440FFX), Ciudad de Buenos Aires, Argentina.

Xanthan is a virulence factor produced by Xanthomonas spp. We previously demonstrated that this exopolysaccharide is not only essential for pathogenicity by contributing with bacterial survival but also its pyruvate substituents interfere with some plant defense responses. Deepening our studies about xanthan properties and structure, the aim of this work was to analyze the characteristics of xanthan produced by Xanthomonas in different culture media. Read More

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http://dx.doi.org/10.1093/glycob/cwy114DOI Listing
March 2019
2 Reads

Trypanosoma cruzi 13C-labeled O-Glycan standards for mass spectrometry.

Glycobiology 2019 04;29(4):280-284

Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA.

Trypanosoma cruzi is a protozoan parasite that causes Chagas disease, a debilitating condition that affects over 10 million humans in the American continents. In addition to its traditional mode of human entry via the "kissing bug" in endemic areas, the infection can also be spread in non-endemic countries through blood transfusion, organ transplantation, eating food contaminated with the parasites, and from mother to fetus. Previous NMR-based studies established that the parasite expresses a variety of strain-specific and developmentally-regulated O-glycans that may contribute to virulence. Read More

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http://dx.doi.org/10.1093/glycob/cwy111DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6422234PMC
April 2019
2 Reads

Alterations in sialic-acid O-acetylation glycoforms during murine erythrocyte development.

Glycobiology 2019 03;29(3):222-228

Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA.

We used Casd1-deficient mice to confirm that this enzyme is responsible for 9-O-acetylation of sialic acids in vivo. We observed a complete loss of 9-O-acetylation of sialic acid on the surface of myeloid, erythroid and CD4+ T cells in Casd1-deficient mice. Although 9-O-acetylation of sialic acids on multiple hematopoietic lineages was lost, there were no obvious defects in hematopoiesis. Read More

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https://academic.oup.com/glycob/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/glycob/cwy110DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381321PMC
March 2019
11 Reads
3.147 Impact Factor

Absence of a human ortholog of rodent Kupffer cell galactose-binding receptor encoded by the CLEC4f gene.

Glycobiology 2019 04;29(4):332-345

Department of Life Sciences, Imperial College, London, UK.

The murine CLEC4f gene encodes the Kupffer cell receptor, a galactose-binding receptor containing a C-type carbohydrate-recognition domain. Orthologs have been identified in nearly 100 species. The receptors from rat and mouse have previously been characterized and data presented here show that functional CLEC4f protein is expressed in domestic cattle (Bos taurus). Read More

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https://academic.oup.com/glycob/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/glycob/cwy113DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6422238PMC
April 2019
10 Reads

Total loss of GM3 synthase activity by a normally processed enzyme in a novel variant and in all ST3GAL5 variants reported to cause a distinct congenital disorder of glycosylation.

Glycobiology 2019 03;29(3):229-241

Department of Medicine and Surgery (DMC), University of Insubria, via JH Dunant 5, Varese, Italy.

ST3GAL5-CDG is a rare syndrome which is caused by variant GM3 synthases, the enzyme involved in the biosynthesis of a-b-c-series gangliosides. Here we report a novel homozygous ST3GAL5 variant, p.Gly342Ser, in a patient suffering from failure to thrive, severe hearing, visual, motor, and cognitive impairment, and respiratory chain dysfunction. Read More

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http://dx.doi.org/10.1093/glycob/cwy112DOI Listing
March 2019
1 Read

Transcription of human β4-galactosyltransferase 3 is regulated by differential DNA binding of Sp1/Sp3 in SH-SY5Y human neuroblastoma and A549 human lung cancer cell lines.

Glycobiology 2019 03;29(3):211-221

Laboratory of Glycobiology, Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, Japan.

Poor prognosis of neuroblastoma patients has been shown to be associated with increased expression of β4-galactosyltransferase (β4GalT) 3. To address the underlying mechanism of the increased expression of β4GalT3, the transcriptional regulation of the human β4GalT3 gene was investigated in SH-SY5Y human neuroblastoma cell line comparing with A549 human lung cancer cell line, in which the β4GalT3 gene expression was the lowest among four cancer cell lines examined. The core promoter region was identified between nucleotides -69 and -6 relative to the transcriptional start site, and the same region was utilized in both cell lines. Read More

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http://dx.doi.org/10.1093/glycob/cwy109DOI Listing
March 2019
1 Read

Active site complementation and hexameric arrangement in the GH family 29; a structure-function study of α-l-fucosidase isoenzyme 1 from Paenibacillus thiaminolyticus.

Glycobiology 2019 01;29(1):59-73

Laboratory of Structure and Function of Biomolecules, Institute of Biotechnology of the Czech Academy of Sciences, v.v.i., Biocev, Vestec, Czech Republic.

α-l-Fucosidase isoenzyme 1 from bacterium Paenibacillus thiaminolyticus is a member of the glycoside hydrolase family GH29 capable of cleaving l-fucose from nonreducing termini of oligosaccharides and glycoconjugates. Here we present the first crystal structure of this protein revealing a novel quaternary state within this family. The protein is in a unique hexameric assembly revealing the first observed case of active site complementation by a residue from an adjacent monomer in this family. Read More

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http://dx.doi.org/10.1093/glycob/cwy078DOI Listing
January 2019
3 Reads

Identification of abnormal fucosylated-glycans recognized by LTL in saliva of HBV-induced chronic hepatitis, cirrhosis, and hepatocellular carcinoma.

Glycobiology 2019 03;29(3):242-259

Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, China.

The hepatitis B virus (HBV)-induced chronic liver diseases are serious health threats worldwide. There is evidence to display the alterations of salivary N-linked glycans related to the development of HBV-infected liver diseases. Here, we further investigated the alterations of fucosylated N/O-glycans recognized by LTL in saliva from 120 subjects (30 healthy volunteers (HV), 30 patients with hepatitis B (HB), 30 patients with hepatic cirrhosis (HC), and 30 patients with hepatocellular carcinoma (HCC)) using salivary microarrys and MALDI-TOF/TOF-MS. Read More

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http://dx.doi.org/10.1093/glycob/cwy108DOI Listing
March 2019
5 Reads

In vitro acellular method to reveal O-fucosylation on EGF-like domains.

Glycobiology 2018 Nov 29. Epub 2018 Nov 29.

Univ. Limoges, PEIRENE, EA 7500, Glycosylation and cell differentiation, F-87000 Limoges, France.

A hundred of human proteins have one or more EGF-like domains (EGF-LD) bearing the O-fucosylation consensus motif C2X4(S/T)C3 but to date, only a few of them have been shown to be O-fucosylated. The protein O-fucosyltransferase (POFUT1) specifically recognizes correctly folded EGF-LD of the human EGF (hEGF) type and transfers fucose on serine or threonine residue within the O-fucosylation motif. Here, we propose a strategy for a rapid screening for ability of any EGF-LD to be O-fucosylated, using copper-catalyzed azide-alkyne cycloaddition (CuAAC). Read More

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http://dx.doi.org/10.1093/glycob/cwy106DOI Listing
November 2018
2 Reads

Globo-series glycosphingolipids enhance Toll-like receptor 4-mediated inflammation and play a pathophysiological role in diabetic nephropathy.

Glycobiology 2019 03;29(3):260-268

Division of Glycopathology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai, Japan.

Alteration of glycosphingolipid (GSL) expression plays key roles in the pathogenesis and pathophysiology of many important human diseases, including cancer, diabetes and glycosphingolipidosis. Inflammatory processes are involved in development and progression of diabetic nephropathy, a major complication of type 2 diabetes mellitus. GSLs are known to play roles in inflammatory responses in various diseases, and levels of renal GSLs are elevated in mouse models of diabetic nephropathy; however, little is known regarding the pathophysiological role of these GSLs in this disease process. Read More

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http://dx.doi.org/10.1093/glycob/cwy105DOI Listing
March 2019
1 Read

Transcriptional activation of fucosyltransferase (FUT) genes using the CRISPR-dCas9-VPR technology reveals potent N-glycome alterations in colorectal cancer cells.

Glycobiology 2019 02;29(2):137-150

Amsterdam UMC, Vrije Universiteit Amsterdam, Department of Molecular Cell Biology and Immunology, Cancer Center Amsterdam, Amsterdam Infection & Immunity Institute, HZ Amsterdam, the Netherlands.

Aberrant fucosylation in cancer cells is considered as a signature of malignant cell transformation and it is associated with tumor progression, metastasis and resistance to chemotherapy. Specifically, in colorectal cancer cells, increased levels of the fucosylated Lewisx antigen are attributed to the deregulated expression of pertinent fucosyltransferases, like fucosyltransferase 4 (FUT4) and fucosyltransferase 9 (FUT9). However, the lack of experimental models closely mimicking cancer-specific regulation of fucosyltransferase gene expression has, so far, limited our knowledge regarding the substrate specificity of these enzymes and the impact of Lewisx synthesis on the glycome of colorectal cancer cells. Read More

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http://dx.doi.org/10.1093/glycob/cwy096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6330019PMC
February 2019
1 Read

A widely compatible expression system for the production of highly O-GlcNAcylated recombinant protein in Escherichia coli.

Glycobiology 2018 12;28(12):949-957

Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao, China.

O-GlcNAcylation is a ubiquitous and dynamic post-translational modification on serine/threonine residues of nucleocytoplasmic proteins in metazoa, which plays a critical role in numerous physiological and pathological processes. But the O-GlcNAcylation on most proteins is often substoichiometric, which hinders the functional study of the O-GlcNAcylation. This study aimed to improve the production of highly O-GlcNAcylated recombinant proteins in Escherichia coli (E. Read More

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http://dx.doi.org/10.1093/glycob/cwy077DOI Listing
December 2018
17 Reads

UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase is indispensable for oogenesis, oocyte-to-embryo transition, and larval development of the nematode Caenorhabditis elegans.

Glycobiology 2019 02;29(2):163-178

Department of Biology, Faculty of Sciences, Kyushu University, Fukuoka, Japan.

N-linked glycosylation of proteins is the most common post-translational modification of proteins. The enzyme UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase (DPAGT1) catalyses the first step of N-glycosylation, and DPAGT1 knockout is embryonic lethal in mice. In this study, we identified the sole orthologue (algn-7) of the human DPAGT1 in the nematode C. Read More

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http://fdslive.oup.com/www.oup.com/pdf/production_in_progres
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http://dx.doi.org/10.1093/glycob/cwy104DOI Listing
February 2019
18 Reads

An efficient use of X-ray information, homology modeling, molecular dynamics and knowledge-based docking techniques to predict protein-monosaccharide complexes.

Glycobiology 2019 02;29(2):124-136

Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Intendente Guiraldes 2160, Ciudad Autónoma de Buenos Aires, Argentina.

Unraveling the structure of lectin-carbohydrate complexes is vital for understanding key biological recognition processes and development of glycomimetic drugs. Molecular Docking application to predict them is challenging due to their low affinity, hydrophilic nature and ligand conformational diversity. In the last decade several strategies, such as the inclusion of glycan conformation specific scoring functions or our developed solvent-site biased method, have improved carbohydrate docking performance but significant challenges remain, in particular, those related to receptor conformational diversity. Read More

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https://academic.oup.com/glycob/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/glycob/cwy102DOI Listing
February 2019
30 Reads

The Lec5 glycosylation mutant links homeobox genes with cholesterol and lipid-linked oligosaccharides.

Glycobiology 2019 02;29(2):106-109

Department of Pharmacology, UT Southwestern Medical Center, 6001 Forest Park Rd., Dallas, TX, USA.

Discovered 40 years ago, the Lec5 glycosylation mutant cell line has a complex recessive genotype and is characterized by accumulation of lipid-linked oligosaccharide assembly intermediates, reduced conversion of polyprenols to dolichols, and an unusual phenotypic dependence upon cell culture conditions such as temperature, plating density and medium quality. The heritable defect in Lec5 is unknown. Here we demonstrate an unexpected epigenetic basis for Lec5, with a surprising linkage to increased expression of homeobox genes, which in turn is associated with increased transcription of cholesterol biosynthesis genes. Read More

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http://dx.doi.org/10.1093/glycob/cwy103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6330018PMC
February 2019
4 Reads
3.150 Impact Factor

Galectin-9 induces atypical ubiquitination leading to cell death in PC-3 prostate cancer cells.

Glycobiology 2019 01;29(1):22-35

Division of Research Instrument and Equipment, Life Science Research Center, Kagawa, Japan.

Galectin-9 is the most potent inducer of cell death in lymphomas and other malignant cell types among the members of the galectin family. We investigated the mechanism of galectin-9-induced cell death in PC-3 prostate cancer cells in comparison with in Jurkat T cells. Galectin-9 induced apoptotic cell death in Jurkat cells, as typically revealed by DNA ladder formation. Read More

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https://academic.oup.com/glycob/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/glycob/cwy099DOI Listing
January 2019
3 Reads

Serine-rich repeat protein adhesins from Lactobacillus reuteri display strain specific glycosylation profiles.

Glycobiology 2019 01;29(1):45-58

The Gut Microbes and Health Institute Strategic Programme, Quadram Institute Bioscience, Norwich Research Park, Norwich, UK.

Lactobacillus reuteri is a gut symbiont inhabiting the gastrointestinal tract of numerous vertebrates. The surface-exposed serine-rich repeat protein (SRRP) is a major adhesin in Gram-positive bacteria. Using lectin and sugar nucleotide profiling of wild-type or L. Read More

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https://academic.oup.com/glycob/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/glycob/cwy100DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6291802PMC
January 2019
6 Reads
3.150 Impact Factor

Elucidation of the O-antigen structure of Escherichia coli O63.

Glycobiology 2019 02;29(2):179-187

Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Stockholm, Sweden.

The structure of the O-antigen polysaccharide (PS) from the Shiga-toxin producing Escherichia coli O63 has been elucidated using a combination of bioinformatics, component analyses and NMR spectroscopy. The O-antigen is comprised of tetrasaccharide repeating units with the following structure: →2)-β-d-Quip3N(d-allo-ThrAc)-(1→2)-β-d-Ribf-(1→4)-β-d-Galp-(1→3)-α-d-GlcpNAc-(1→ in which the N-acetylated d-allo-threonine is amide-linked to position 3 of the 3-amino-3-deoxy-d-Quip sugar residue. The presence of a predicted flippase and polymerase encoded in the O63 gene cluster is consistent with the Wzx/Wzy biosynthetic pathway and consequently the biological repeating unit has likely an N-acetyl-d-glucosamine residue at its reducing end. Read More

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https://academic.oup.com/glycob/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/glycob/cwy098DOI Listing
February 2019
13 Reads

Sequence-to-structure dependence of isolated IgG Fc complex biantennary N-glycans: a molecular dynamics study.

Glycobiology 2019 01;29(1):94-103

Department of Chemistry.

Fc glycosylation of human immunoglobulins G (IgGs) is essential for their structural integrity and activity. Interestingly, the specific nature of the Fc glycoforms is known to modulate the IgG effector function and inflammatory properties. Indeed, while core-fucosylation of IgG Fc-glycans greatly affects the antibody-dependent cell-mediated cytotoxicity function, with obvious repercussions in the design of therapeutic antibodies, sialylation can reverse the antibody inflammatory response, and galactosylation levels have been linked to aging, to the onset of inflammation, and to the predisposition to rheumatoid arthritis. Read More

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http://dx.doi.org/10.1093/glycob/cwy097DOI Listing
January 2019
26 Reads

Oligosaccharyltransferase structures provide novel insight into the mechanism of asparagine-linked glycosylation in prokaryotic and eukaryotic cells.

Glycobiology 2019 04;29(4):288-297

Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA, USA.

Asparagine-linked (N-linked) glycosylation is one of the most common protein modification reactions in eukaryotic cells, occurring upon the majority of proteins that enter the secretory pathway. X-ray crystal structures of the single subunit OSTs from eubacterial and archaebacterial organisms revealed the location of donor and acceptor substrate binding sites and provided the basis for a catalytic mechanism. Cryoelectron microscopy structures of the octameric yeast OST provided substantial insight into the organization and assembly of the multisubunit oligosaccharyltransferases. Read More

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https://academic.oup.com/glycob/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/glycob/cwy093DOI Listing
April 2019
13 Reads

Society for Glycobiology Awards - 2018.

Authors:

Glycobiology 2018 12;28(12):906-909

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http://dx.doi.org/10.1093/glycob/cwy086DOI Listing
December 2018
1 Read

Helicobacter pylori induces intracellular galectin-8 aggregation around damaged lysosomes within gastric epithelial cells in a host O-glycan-dependent manner.

Glycobiology 2019 02;29(2):151-162

Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.

Galectin-8, a beta-galactoside-binding lectin, is upregulated in the gastric tissues of rhesus macaques infected with Helicobacter pylori. In this study, we found that H. pylori infection triggers intracellular galectin-8 aggregation in human-derived AGS gastric epithelial cells, and that these aggregates colocalize with lysosomes. Read More

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https://academic.oup.com/glycob/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/glycob/cwy095DOI Listing
February 2019
8 Reads