9,288 results match your criteria Glycobiology [Journal]


Extracellular polymeric substances of biofilms: Suffering from an identity crisis.

Water Res 2018 Nov 28;151:1-7. Epub 2018 Nov 28.

Department of Biotechcnology, Delft University of Technology, Delft, the Netherlands. Electronic address:

Microbial biofilms can be both cause and cure to a range of emerging societal problems including antimicrobial tolerance, water sanitation, water scarcity and pollution. The identities of extracellular polymeric substances (EPS) responsible for the establishment and function of biofilms are poorly understood. The lack of information on the chemical and physical identities of EPS limits the potential to rationally engineer biofilm processes, and impedes progress within the water and wastewater sector towards a circular economy and resource recovery. Read More

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November 2018

An Integrated Pipeline of Isotopic Labeling and Selective Enriching for Quantitative Analysis of N-Glycome by Mass Spectrometry.

Anal Chem 2018 Dec 17. Epub 2018 Dec 17.

Quantitative N-glycomics can reveal abnormal expression of N-glycan in diseases. However, mass spectrometry (MS)-based N-glycome quantitative analysis is still technically challenging. To achieve the quantitation of N-glycome with high accuracy and sensitivity, it is required to efficiently label the N-glycans with isotopic tags and selectively enrich N-glycans to avoid suppression from other substances. Read More

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December 2018

Prolactin-induced protein (PIP)-characterization and role in breast cancer progression.

Am J Cancer Res 2018 1;8(11):2150-2164. Epub 2018 Nov 1.

Department of Human Morphology, Division of Histology and Embryology, Wroclaw Medical University Wroclaw, Poland.

Prolactin-induced protein (PIP) is a small secreted glycoprotein carrying several N-linked carbohydrate chains. The expression of PIP is generally restricted to cells with apocrine properties. It was found in apocrine glands of the axilla, vulva, eyelid, ear canal, and seminal vesicle. Read More

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November 2018

Influenza Virus N-linked Glycosylation and Innate Immunity.

Biosci Rep 2018 Dec 14. Epub 2018 Dec 14.

Influenza Division, Centers for Disease Control and Prevention, Atlanta, Georgia, 30324, United States

Influenza viruses cause seasonal epidemics and sporadic pandemics in humans. The virus's ability to change its antigenic nature through mutation and recombination, and the difficulty in developing highly effective universal vaccines against it, make it a serious global public health challenge. Influenza virus's surface glycoproteins, hemagglutinin and neuraminidase, are all modified by the host cell's N-linked glycosylation pathways. Read More

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December 2018
1 Read

The serogroup B meningococcal vaccine Bexsero elicits antibodies to Neisseria gonorrhoeae.

Clin Infect Dis 2018 Dec 14. Epub 2018 Dec 14.

Institute for Glycomics, Griffith University, Gold Coast, Australia.

Background: Neisseria gonorrhoeae and Neisseria meningitidis are closely related bacteria that cause a significant global burden of disease. Control of gonorrhoea is becoming increasingly difficult due to widespread antibiotic resistance. While vaccines are routinely used for N. Read More

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December 2018
1 Read

Meeting and Course Announcements.

Authors:

Glycobiology 2019 Jan;29(1)

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January 2019

Active site complementation and hexameric arrangement in the GH family 29; a structure-function study of α-l-fucosidase isoenzyme 1 from Paenibacillus thiaminolyticus.

Glycobiology 2019 Jan;29(1):59-73

Laboratory of Structure and Function of Biomolecules, Institute of Biotechnology of the Czech Academy of Sciences, v.v.i., Biocev, Vestec, Czech Republic.

α-l-Fucosidase isoenzyme 1 from bacterium Paenibacillus thiaminolyticus is a member of the glycoside hydrolase family GH29 capable of cleaving l-fucose from nonreducing termini of oligosaccharides and glycoconjugates. Here we present the first crystal structure of this protein revealing a novel quaternary state within this family. The protein is in a unique hexameric assembly revealing the first observed case of active site complementation by a residue from an adjacent monomer in this family. Read More

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January 2019

Esculentoside H inhibits colon cancer cell migration and growth through suppression of MMP-9 gene expression via NF-kB signaling pathway.

J Cell Biochem 2018 Dec 7. Epub 2018 Dec 7.

Molecular and Cellular Glycobiology Unit, Department of Biological Sciences, SungKyunKwan University, Chunchun-Dong, Jangan-Gu, Suwon, Kyunggi-Do, Korea.

A water-soluble saponin, Esculentoside H (EsH), 3-O-(O-β-d-glucopyranosyl-(1→4)-β-d-xylopyranosyl)-28-β-d-glucopyranosylphytolaccagenin has been isolated and purified from the root extract of perennial plant Phytolacca esculenta. EsH is known to be an anticancer compound, having a capacity for TNF-α release. However, the effects of EsH on migration and growth in tumor cells have not yet been reported. Read More

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December 2018

Cryo-EM Structures of Eastern Equine Encephalitis Virus Reveal Mechanisms of Virus Disassembly and Antibody Neutralization.

Cell Rep 2018 Dec;25(11):3136-3147.e5

Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA. Electronic address:

Alphaviruses are enveloped pathogens that cause arthritis and encephalitis. Here, we report a 4.4-Å cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans. Read More

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December 2018
8 Reads

Analysis of Anti-C1q Autoantibodies by Western Blot.

Methods Mol Biol 2019 ;1901:183-189

Clinical Immunology and Transfusion Medicine, Region Skåne, Sweden.

Anti-C1q autoantibodies may be found in many conditions, most commonly in systemic lupus erythematosus (SLE) and hypocomplementemic urticarial vasculitis syndrome (HUVS), and are diagnostic markers as well as disease activity markers in lupus nephritis. Sera from patients with SLE and HUVS show partly distinct autoantibody reactivities to separated protein chains B and C of the first component of complement, C1q. These different binding specificities can be detected by Western blot analysis of the autoantibodies under reducing conditions. Read More

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January 2019

Analysis of C3 Nephritic Factors by ELISA.

Authors:
Lillemor Skattum

Methods Mol Biol 2019 ;1901:177-182

Department of Laboratory Medicine, Section of Microbiology, Immunology and Glycobiology, Lund University, Lund, Sweden.

Autoantibodies to the C3 convertase of the alternative pathway of complement, called C3 nephritic factors (C3NeF), cause persistently low C3 in the circulation and production of C3 degradation fragments due to prolonged stabilization of the C3 convertase. C3NeF are associated with glomerulopathy, acquired partial lipodystrophy, and less frequently with increased susceptibility to meningococcal infection. Analysis of C3NeF is an important part of the diagnostic workup of C3 glomerulopathy, but their identification is difficult presumably due to considerable heterogeneity. Read More

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January 2019

Chemical Synergy between Ionophore PBT2 and Zinc Reverses Antibiotic Resistance.

MBio 2018 Dec 11;9(6). Epub 2018 Dec 11.

School of Chemistry and Molecular Biosciences and Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, QLD, Australia

The World Health Organization reports that antibiotic-resistant pathogens represent an imminent global health disaster for the 21st century. Gram-positive superbugs threaten to breach last-line antibiotic treatment, and the pharmaceutical industry antibiotic development pipeline is waning. Here we report the synergy between ionophore-induced physiological stress in Gram-positive bacteria and antibiotic treatment. Read More

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December 2018

Glycoblotting of Egg White Reveals Diverse N-glycan Expression in Quail Species.

J Agric Food Chem 2018 Dec 11. Epub 2018 Dec 11.

The glycan part of glycoproteins is known to be involved in the structure and modulatory functions of glycoproteins, serving as ligands for cell to cell interactions, and as specific ligands for cell to microbe interactions. It is believed that intraspecies and interspecies variations in glycosylation exist. However, only a few information about glycan diversity is available. Read More

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December 2018
2 Reads

Mitochondrial Function Modulates Touch Signalling in Arabidopsis thaliana.

Plant J 2018 Dec 8. Epub 2018 Dec 8.

Department of Animal, Plant and Soil Science, School of Life Science, Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, 5 Ring Road, Bundoora, 3086, Victoria, Australia.

Plants respond to short- and long-term mechanical stimuli, via altered transcript abundance and growth respectively. Jasmonate, gibberellic acid and calcium have been implicated in mediating responses to mechanical stimuli. Previously it was shown that the transcript abundance for the outer mitochondrial membrane protein of 66 kDa (OM66), is induced several-fold after 30 min in response to touch. Read More

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December 2018
1 Read

New horizons in Glycobiology research.

Authors:
Sandro Sonnino

FEBS Lett 2018 Dec;592(23):3771-3772

Department of Medical Biotechnology and Translational Medicine, University of Milan, Italy.

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December 2018

Identification of Abnormal Fucosylated-Glycans Recognized by LTL in Saliva of HBV-Induced Chronic Hepatitis, Cirrhosis, and Hepatocellular Carcinoma.

Glycobiology 2018 Dec 7. Epub 2018 Dec 7.

Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, China.

The hepatitis B virus (HBV)-induced chronic liver diseases are serious health threats worldwide. There is evidence to display the alterations of salivary N-linked glycans related to the development of HBV-infected liver diseases. Here, we further investigated the alterations of fucosylated N/O-glycans recognized by LTL in saliva from 120 subjects (30 healthy volunteers (HV), 30 patients with hepatitis B (HB), 30 patients with hepatic cirrhosis (HC), and 30 patients with hepatocellular carcinoma (HCC)) using salivary microarrys and MALDI-TOF/TOF-MS. Read More

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December 2018

Improving Prediction of Protein Secondary Structure, Backbone Angles, Solvent Accessibility, and Contact Numbers by Using Predicted Contact Maps and an Ensemble of Recurrent and Residual Convolutional Neural Networks.

Bioinformatics 2018 Dec 7. Epub 2018 Dec 7.

School of Information and Communication Technology, Griffith University, Gold Coast, Australia.

Motivation: Sequence-based prediction of one dimensional structural properties of proteins has been a long-standing subproblem of protein structure prediction. Recently, prediction accuracy has been significantly improved due to the rapid expansion of protein sequence and structure libraries and advances in deep learning techniques, such as residual convolutional networks (ResNets) and Long-Short-Term Memory Cells in Bidirectional Recurrent Neural Networks (LSTM-BRNNs). Here we leverage an ensemble of LSTM-BRNN and ResNet models, together with predicted residue-residue contact maps, to continue the push towards the attainable limit of prediction for 3- and 8-state secondary structure, backbone angles (θ, τ, ϕ, and ψ), half-sphere exposure, contact numbers, and solvent accessible surface area (ASA). Read More

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December 2018
1 Read

Cross-Species Immune Recognition Between Plasmodium vivax Duffy Binding Protein Antibodies and the Plasmodium falciparum Surface Antigen VAR2CSA.

J Infect Dis 2019 Jan;219(1):110-120

School of Public Health, University of Alberta, Edmonton, Canada.

Background: In pregnancy, Plasmodium falciparum parasites express the surface antigen VAR2CSA, which mediates adherence of red blood cells to chondroitin sulfate A (CSA) in the placenta. VAR2CSA antibodies are generally acquired during infection in pregnancy and are associated with protection from placental malaria. We observed previously that men and children in Colombia also had antibodies to VAR2CSA, but the origin of these antibodies was unknown. Read More

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January 2019

Curcumin Downregulates Human GM3 Synthase (hST3Gal V) Gene Expression with Autophagy Induction in Human Colon Carcinoma HCT116 Cells.

Evid Based Complement Alternat Med 2018 11;2018:6746412. Epub 2018 Nov 11.

Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan 49315, Republic of Korea.

Our recent report showed that curcumin, polyphenolic compound isolated from the herb , upregulated the gene expression of human GD3 synthase (hST8Sia I) responsible for ganglioside GD3 synthesis with autophagy induction in human lung adenocarcinoma A549 cells. In this study, on the contrary to this finding, we demonstrated that curcumin downregulated the gene expression of human GM3 synthase (hST3Gal V) catalyzing ganglioside GM3 synthesis with autophagy induction in human colon carcinoma HCT116 cells. To clarify the mechanism leading to the downregulation of hST3Gal V gene expression in curcumin-treated HCT116 cells, we analyzed the curcumin-inducible promoter of the hST3Gal V gene by luciferase reporter assays. Read More

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November 2018
3 Reads

Closed Complete Genome Sequences of Two Nontypeable Haemophilus influenzae Strains Containing Novel Alleles from the Sputum of Patients with Chronic Obstructive Pulmonary Disease.

Microbiol Resour Announc 2018 Jul 19;7(2). Epub 2018 Jul 19.

Institute for Glycomics, Griffith University, Gold Coast, Queensland, Australia.

Nontypeable Haemophilus influenzae (NTHi) is an important bacterial pathogen that causes otitis media and exacerbations of chronic obstructive pulmonary disease (COPD). Here, we report the complete genome sequences of NTHi strains 10P129H1 and 84P36H1, isolated from COPD patients, which contain the phase-variable epigenetic regulators ModA15 and ModA18, respectively. Read More

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Lectin activity of the TcdA and TcdB toxins of .

Infect Immun 2018 Dec 10. Epub 2018 Dec 10.

Institute for Glycomics, Griffith University, Gold Coast, QLD 4222, Australia.

is a major cause of hospital-acquired antibiotic-associated diarrhoea. produces two cytotoxins, TcdA and TcdB, both toxins are multi-domain proteins that lead to cytotoxicity through the modification and inactivation of small GTPases of the Rho/Rac family. Previous studies have indicated that host glycans are targets for TcdA and TcdB, with interactions thought to be with both α- and β-linked galactose. Read More

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December 2018

Unbiased glycomics: a powerful tool in rare disease diagnosis and research.

Transl Res 2018 Nov 24. Epub 2018 Nov 24.

Department of Molecular and Human Genetics, Program in Developmental Biology, Baylor College of Medicine, Houston, Texas. Electronic address:

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November 2018

The polyphenolic-polysaccharide complex of Agrimonia eupatoria L. as an indirect thrombin inhibitor - isolation and chemical characterization.

Int J Biol Macromol 2018 Dec 3. Epub 2018 Dec 3.

Department of Organic and Pharmaceutical Technology, Faculty of Chemistry, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 29, 50-370 Wrocław, Poland; Regional Specialist Hospital in Wrocław, Research and Development Centre, Kamieńskiego 73a, 51-124 Wrocław, Poland.

The polyphenolic-polysaccharide complex was isolated from the dried aerial parts of the medicinal plant Agrimonia eupatoria L. using a multi-step process involving the degreasing of the plant material by extraction with organic solvents, followed by extraction with hot alkali, neutralization, further separation with organic solvents and dialysis. The complex was homogeneous with a molecular weight of about 55 × 10 g/mol and consisted mainly of carbohydrates and polyphenols matrix, composed of lignin-related units, with the dominance of dimethoxyphenyl structures. Read More

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December 2018
2 Reads

Persistent reduction in sialylation of cerebral glycoproteins following postnatal inflammatory exposure.

J Neuroinflammation 2018 Dec 5;15(1):336. Epub 2018 Dec 5.

Department of Paediatrics, Sainte-Justine Hospital Research Center, Université de Montréal, Montreal, H3T 1C5, QC, Canada.

Background: The extension of sepsis encompassing the preterm newborn's brain is often overlooked due to technical challenges in this highly vulnerable population, yet it leads to substantial long-term neurodevelopmental disabilities. In this study, we demonstrate how neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups results in persistent reduction in sialylation of cerebral glycoproteins. Read More

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December 2018
1 Read
5.408 Impact Factor

Transurethral resection of the prostate: Are we following the guidelines? - Outcomes from the Global Prevalence of Infections in Urology (GPIU) study.

J Chemother 2018 Dec 3:1-5. Epub 2018 Dec 3.

k Department of Urology, Paediatric Urology and Andrology , Justus-Liebig-University , Gießen , Germany.

Transurethral resection of the prostate (TURP) is one of the most common urological procedures. With the increasing rate of multiresistant infections including urosepsis, it is essential for all surgeons to adhere to the relevant international guidelines to prevent infectious complications. The aim of this prospective, multinational, multicentre study was to evaluate compliance with recommended infection control measures regarding TURP procedures. Read More

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December 2018
5 Reads

Site-Selective Functionalization of Hydroxyl Groups in Carbohydrate Derivatives.

Chem Rev 2018 Dec 3. Epub 2018 Dec 3.

Department of Chemistry , University of Toronto , 80 St. George Street , Toronto , ON M5S 3H6 , Canada.

Methods for site-selective transformations of hydroxyl groups in carbohydrate derivatives are reviewed. The construction of oligosaccharides of defined connectivity hinges on such transformations, which are also needed for the preparation of modified or non-natural sugar derivatives, the installation of naturally occurring postglycosylation modifications, the selective labeling or conjugation of carbohydrate derivatives, and the preparation of therapeutic agents or research tools for glycobiology. The review begins with a discussion of intrinsic factors and processes that can influence selectivity in reactions of unprotected or partially protected carbohydrate derivatives, followed by a description of transformations that engage two OH groups in cyclic adducts (acetals, ketals, boronic esters, and related species). Read More

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December 2018

Immobilization of glycans on solid surfaces for application in glycomics.

J Carbohydr Chem 2018 27;37(4):225-249. Epub 2018 Apr 27.

Department of Chemistry and Biochemistry, University of Missouri - St. Louis, One University Boulevard, St. Louis, Missouri, USA.

Carbohydrates are an important class of biomolecules which are involved in a multitude of cellular functions. In the field of glycomics, the structure and function of various carbohydrates, oligosaccharides, glycans and their conjugates are constantly under investigation. In the continuing quest to understand the roles of carbohydrates in their interactions with proteins, immunogens, and other cell-surface carbohydrates, scientists have developed methods for observing the effects of specific saccharide sequences on various cellular components. Read More

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[Application of Lectin from Catfish Eggs to Cancer Therapy: A Fundamental Study].

Authors:
Shigeki Sugawara

Yakugaku Zasshi 2018 ;138(12):1451-1459

Division of Cell Recognition Study, Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University.

Silurus asotus egg lectin (SAL) is an α-galactoside-binding protein, isolated from the egg of catfish. It belongs to the rhamnose-binding lectin family that binds to Gb3 glycan (Galα1-4Galβ1-4Glc). SAL has resulted in the induction of early apoptosis in the Raji cell line, which is a Burkitt's lymphoma cell line expressing Gb3. Read More

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January 2018
1 Read

Effects of Global -GlcNAcylation on Galectin Gene-expression Profiles in Human Cancer Cell Lines.

Anticancer Res 2018 Dec;38(12):6691-6697

Department of Biology, The University of Western Ontario, London, ON, Canada

Background/aim: The effects of O-linked β-N-acetyl-D-glucosamine (O-GlcNAc) transferase (OGT) and O-GlcNAcase (OGA) inhibitors on galectin gene expression profiles were examined in MCF7, HT-29, and HL-60 cancer cell lines.

Materials And Methods: Cell cultures were treated for 24 h with OGA inhibitor thiamet G or OGT inhibitor 2-acetamido-1,3,4,6-tetra-O-acetyl-2-deoxy-5-thio-α-D-glucopyranose, and global O-GlcNAc levels and expression of galectin genes were determined using an immunodot blot assay and real-time quantitative polymerase chain reaction.

Results: Two galectin genes, LGALS3 in MCF7 cells and LGALS12 in HL-60 cells, were up-regulated by O-GlcNAc, whereas other cell-specific galectins were unresponsive to changes in O-GlcNAc level. Read More

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December 2018

Death, TIR, and RHIM: Self-assembling domains involved in innate immunity and cell-death signaling.

J Leukoc Biol 2018 Dec 5. Epub 2018 Dec 5.

School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience and Australian Infectious Diseases Research Centre, University of Queensland, Brisbane, Queensland, 4072, Australia.

The innate immune system consists of pattern recognition receptors (PRRs) that detect pathogen- and endogenous danger-associated molecular patterns (PAMPs and DAMPs), initiating signaling pathways that lead to the induction of cytokine expression, processing of pro-inflammatory cytokines, and induction of cell-death responses. An emerging concept in these pathways and associated processes is signaling by cooperative assembly formation (SCAF), which involves formation of higher order oligomeric complexes, and enables rapid and strongly amplified signaling responses to minute amounts of stimulus. Many of these signalosomes assemble through homotypic interactions of members of the death-fold (DF) superfamily, Toll/IL-1 receptor (TIR) domains, or the RIP homotypic interaction motifs (RHIM). Read More

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December 2018
2 Reads

Age and Sex-Associated Changes of Complement Activity and Complement Levels in a Healthy Caucasian Population.

Front Immunol 2018 20;9:2664. Epub 2018 Nov 20.

Division of Nephrology, Department of Internal Medicine, University of Groningen, University Medical Center Groningen, Groningen, Netherlands.

The complement system is essential for an adequate immune response. Much attention has been given to the role of complement in disease. However, to better understand complement in pathology, it is crucial to first analyze this system under different physiological conditions. Read More

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November 2018
1 Read

The LPG1x family from Leishmania major is constituted of rare eukaryotic galactofuranosyltransferases with unprecedented catalytic properties.

Sci Rep 2018 Dec 4;8(1):17566. Epub 2018 Dec 4.

Institut de Chimie Organique et Analytique, UMR CNRS 7311, Université d'Orléans, Rue de Chartres, BP6759, Orléans, Cedex 02, France.

Galactofuranosyltransferases are poorly described enzymes despite their crucial role in the virulence and the pathogenicity of numerous microorganisms. These enzymes are considered as potential targets for therapeutic action. In addition to the only well-characterised prokaryotic GlfT2 from Mycobacterium tuberculosis, four putative genes in Leishmania major were previously described as potential galactofuranosyltransferases. Read More

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December 2018
1 Read

A novel derivative of the fungal antimicrobial peptide plectasin is active against Mycobacterium tuberculosis.

Tuberculosis (Edinb) 2018 Dec 30;113:231-238. Epub 2018 Oct 30.

Department of Microbiology, Immunology and Glycobiology, Institution of Laboratory Medicine, Lund University, Lund, Sweden. Electronic address:

Tuberculosis has been reaffirmed as the infectious disease causing most deaths in the world. Co-infection with HIV and the increase in multi-drug resistant Mycobacterium tuberculosis strains complicate treatment and increases mortality rates, making the development of new drugs an urgent priority. In this study we have identified a promising candidate by screening antimicrobial peptides for their capacity to inhibit mycobacterial growth. Read More

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December 2018

Protein Residue Networks from Energetic and Geometric Data: Are They Identical?

J Chem Theory Comput 2018 Nov 30. Epub 2018 Nov 30.

Center for Computational Sciences , University of Tsukuba , Tennodai 1-1-1 , Tsukuba, Ibaraki 305-8577 , Japan.

Protein residue networks (PRN) from energetic and geometric data are probably not identical. PRNs constructed from ab initio pair interaction energies are analyzed for the first time and compared to PRN based on center of mass separation. We use modern, previously unused algorithms such as global and local efficiencies to quantitatively confirm that both types of PRNs do exhibit small-world character. Read More

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November 2018

Novel Dextran-Supported Biological Probes Decorated with Disaccharide Entities for Investigating the Carbohydrate-Protein Interactions of Gal-3.

Chembiochem 2018 Nov 30. Epub 2018 Nov 30.

Johan Gadolin Process Chemistry Centre, Laboratory of Organic Chemistry, Åbo Akademi University, 20500, Turku, Finland.

The quest for novel natural-like biomolecular probes that can be used to gain information on biological recognition events is of topical interest to several scientific areas. In particular, the recognition of carbohydrates by proteins modulates a number of important biological processes. These molecular recognition events are, however, difficult to study by the use of naturally occurring oligosaccharides and polysaccharides owing to their intrinsic structural heterogeneity and to the many technical difficulties encountered during the isolation of sufficient quantities of pure material for detailed structural and biological studies. Read More

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November 2018
1 Read

Histochemical localization of N-acetylhexosamine-binding lectin HOL-18 in Halichondria okadai (Japanese black sponge), and its antimicrobial and cytotoxic anticancer effects.

Int J Biol Macromol 2018 Nov 26;124:819-827. Epub 2018 Nov 26.

Laboratory of Glycobiology and Marine Biochemistry, Department of Life and Environmental System Science, Graduate School of NanoBiosciences, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, Japan. Electronic address:

We studied localization and physiological activities of a lectin showing specific binding to N-acetylhexosamines, termed HOL-18, purified from Japanese black sponge (Halichondria okadai). Antiserum against the lectin was generated in rabbit and applied for immunohistochemical analyses. HOL-18 was expressed specifically around water pores and on spicules of sponge tissues. Read More

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November 2018
1 Read
2.858 Impact Factor

In vitro acellular method to reveal O-fucosylation on EGF-like domains.

Glycobiology 2018 Nov 29. Epub 2018 Nov 29.

Univ. Limoges, PEIRENE, EA 7500, Glycosylation and cell differentiation, F-87000 Limoges, France.

A hundred of human proteins have one or more EGF-like domains (EGF-LD) bearing the O-fucosylation consensus motif C2X4(S/T)C3 but to date, only a few of them have been shown to be O-fucosylated. The protein O-fucosyltransferase (POFUT1) specifically recognizes correctly folded EGF-LD of the human EGF (hEGF) type and transfers fucose on serine or threonine residue within the O-fucosylation motif. Here, we propose a strategy for a rapid screening for ability of any EGF-LD to be O-fucosylated, using copper-catalyzed azide-alkyne cycloaddition (CuAAC). Read More

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November 2018

Identification of Key Residues in Proteins Through Centrality Analysis and Flexibility Prediction with RINspector.

Curr Protoc Bioinformatics 2018 Nov 29:e66. Epub 2018 Nov 29.

University of Lille, CNRS UMR8576 UGSF, Institute for Structural and Functional Glycobiology, F-59000 Lille, France.

Protein structures inherently contain information that can be used to decipher their functions, but the exploitation of this knowledge is not trivial. We recently developed an app for the Cytoscape network visualization and analysis program, called RINspector, the goal of which is to integrate two different approaches that identify key residues in a protein structure or complex. The first approach consists of calculating centralities on a residue interaction network (RIN) generated from the three-dimensional structure; the second consists of predicting backbone flexibility and needs only the primary sequence. Read More

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November 2018

Isomeric Separation and Characterisation of Glycoconjugates.

Adv Exp Med Biol 2018 ;1104:77-99

Institute for Glycomics, Griffith University, Southport, QLD, Australia.

Individual monosaccharides can be linked in a variety of different combinations to form complex glycoconjugates. In contrast to DNA and proteins, glycoconjugate synthesis does not follow any template but is the consequence of the concerted action of various enzymes such as transferases and glycosidases . Thus, tools for glycoconjugate sequencing need to differentiate individual monosaccharide identity, linkage and anomericity to investigate and understand glycoconjugate function. Read More

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January 2018

MALDI Mass Spectrometry Imaging of N-Linked Glycans in Tissues.

Adv Exp Med Biol 2018 ;1104:59-76

Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, SC, USA.

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used for two decades to profile the glycan constituents of biological samples. An adaptation of the method to tissues, MALDI mass spectrometry imaging (MALDI-MSI), allows high-throughput spatial profiling of hundreds to thousands of molecules within a single thin tissue section. The ability to profile N-glycans within tissues using MALDI-MSI is a recently developed method that allows identification and localization of 40 or more N-glycans. Read More

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January 2018

Tunicamycin enhances the suppressive effects of cisplatin on lung cancer growth through PTX3 glycosylation via AKT/NF-κB signaling pathway.

Int J Oncol 2018 Nov 28. Epub 2018 Nov 28.

Department of Biochemistry and Molecular Biology, Liaoning Provincial Core Laboratory of Glycobiology and Glycoengineering, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.

Long pentraxin‑3 (PTX3) is an inflammatory molecule related to cancer proliferation, invasion, and metastasis. Many studies have highlighted the significance of glycosylated molecules in immune modulation, inflammation and cancer progression. Moreover, aberrant glycosylation of cancer cells is linked to chemoresistance. Read More

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November 2018
1 Read
3.025 Impact Factor

Development of a colorimetric PNGase activity assay.

Carbohydr Res 2018 Nov 16;472:58-64. Epub 2018 Nov 16.

Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China. Electronic address:

PNGases are crucial targets and valuable tools in analyzing asparagine-linked carbohydrate moieties (N-glycans) of glycoproteins. Activity tests of PNGases have been little improved since their discovery four decades ago, and still rely on observing deglycosylation patterns of glycoproteins or glycopeptides using SDS-PAGE or HPLC analysis. These techniques cannot be easily adapted for automated sampling and high-throughput procedures. Read More

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November 2018
4 Reads

The role of chemotaxis during Campylobacter jejuni colonisation and pathogenesis.

Authors:
Victoria Korolik

Curr Opin Microbiol 2018 Nov 23;47:32-37. Epub 2018 Nov 23.

Institute for Glycomics, Griffith University, Gold Coast, 4222, QLD, Australia. Electronic address:

Campylobacter jejuni is a ubiquitous gastrointestinal pathogen, transmitted to humans from birds and animals, where C. jejuni is part of normal intestinal flora. In C. Read More

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November 2018
3 Reads

Globo-series glycosphingolipids enhance Toll-like receptor 4-mediated inflammation and play a pathophysiological role in diabetic nephropathy.

Glycobiology 2018 Nov 22. Epub 2018 Nov 22.

Division of Glycopathology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai, Japan.

Alteration of glycosphingolipid (GSL) expression plays key roles in the pathogenesis and pathophysiology of many important human diseases, including cancer, diabetes, and glycosphingolipidosis. Inflammatory processes are involved in development and progression of diabetic nephropathy, a major complication of type 2 diabetes mellitus. GSLs are known to play roles in inflammatory responses in various diseases, and levels of renal GSLs are elevated in mouse models of diabetic nephropathy; however, little is known regarding the pathophysiological role of these GSLs in this disease process. Read More

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November 2018

Transcriptional activation of fucosyltransferase (FUT) genes using the CRISPR-dCas9-VPR technology reveals potent N-glycome alterations in colorectal cancer cells.

Glycobiology 2018 Nov 22. Epub 2018 Nov 22.

Amsterdam UMC, Vrije Universiteit Amsterdam, Department of Molecular Cell Biology and Immunology, Cancer Center Amsterdam, Amsterdam Infection & Immunity Institute, 1081 HZ Amsterdam, the Netherlands.

Aberrant fucosylation in cancer cells is considered as a signature of malignant cell transformation and it is associated with tumor progression, metastasis and resistance to chemotherapy. Specifically, in colorectal cancer cells, increased levels of the fucosylated Lewisx antigen are attributed to the deregulated expression of pertinent fucosyltransferases, like fucosyltransferase 4 (FUT4) and fucosyltransferase 9 (FUT9). However, the lack of experimental models closely mimicking cancer-specific regulation of fucosyltransferase gene expression has, so far, limited our knowledge regarding the substrate specificity of these enzymes and the impact of Lewisx synthesis on the glycome of colorectal cancer cells. Read More

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November 2018

Improvement of electrospray stability in negative ion mode for nano-PGC-LC-MS glycoanalysis via post-column make-up flow.

Glycoconj J 2018 12 23;35(6):499-509. Epub 2018 Nov 23.

Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106, Magdeburg, Germany.

Analysis of glycans via a porous graphitized carbon liquid chromatography (PGC-LC) coupled with electrospray ionization (tandem) mass spectrometry (ESI-MS(/MS)) is a powerful analytical method in the field of glycomics. Isobaric glycan structures can be identified reliably with the help of PGC-LC separation and subsequent identification by ESI-MS(/MS) in negative ion mode. In an effort to adapt PGC-LC-ESI-MS(/MS) to the nano-scale operation, spray instability along the nano-PGC-LC gradient was repeatedly observed on an LTQ Orbitrap Elite mass spectrometer equipped with a standard nano-electrospray ionization source. Read More

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December 2018

CRISPR/Cas9 and glycomics tools for glycobiology.

J Biol Chem 2018 Nov 21. Epub 2018 Nov 21.

Biochemistry & Molecular Biology, University of Georgia, United States.

Infection with the protozoan parasite is a major health risk owing to birth defects, its chronic nature, ability to reactivate to cause blindness and encephalitis, and high prevalence in human populations. Unlike most eukaryotes, propagates in intracellular parasitophorous vacuoles, but as for nearly all other eukaryotes, Toxoplasma glycosylates many cellular proteins and lipids and assembles polysaccharides. glycans resemble those of other eukaryotes but species-specific variations have prohibited deeper investigations into their roles in parasite biology and virulence. Read More

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November 2018
2 Reads

Structural enzymology reveals the molecular basis of substrate regiospecificity and processivity of an exemplar bacterial Glycoside Hydrolase Family 74 endo-xyloglucanase.

Biochem J 2018 Nov 21. Epub 2018 Nov 21.

University of British Columbia, Vancouver, Canada

produces a single multimodular enzyme containing a glycoside hydrolase (GH) family 74 module (AIQ73809). Recombinant production and characterization of the GH74 module ( GH74) revealed a highly specific, processive -xyloglucanase that is able to hydrolyze the polysaccharide backbone at both branched and unbranched positions. X-ray crystal structures obtained for the free enzyme and oligosaccharide complexes evidenced an extensive hydrophobic binding platform - the first in GH74 extending from subsites -4 to +6 - and unique mobile active-site loops. Read More

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November 2018
1 Read

A widely compatible expression system for the production of highly O-GlcNAcylated recombinant protein in Escherichia coli.

Glycobiology 2018 12;28(12):949-957

Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao, China.

O-GlcNAcylation is a ubiquitous and dynamic post-translational modification on serine/threonine residues of nucleocytoplasmic proteins in metazoa, which plays a critical role in numerous physiological and pathological processes. But the O-GlcNAcylation on most proteins is often substoichiometric, which hinders the functional study of the O-GlcNAcylation. This study aimed to improve the production of highly O-GlcNAcylated recombinant proteins in Escherichia coli (E. Read More

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December 2018
5 Reads