6,955 results match your criteria Glucose-6-Phosphatase Deficiency


Effects of dietary methionine restriction on postnatal growth, insulin sensitivity, and glucose metabolism in intrauterine growth retardation pigs at 49 and 105 days of age.

J Anim Sci 2018 Dec 2. Epub 2018 Dec 2.

College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.

This study was conducted to investigate the effects of methionine restriction (MR) on growth performance, insulin sensitivity, and hepatic and muscle glucose metabolism in intrauterine growth retardation (IUGR) pigs at 49 and 105 days of age. At weaning (day 21), 30 female normal birth weight (NBW) piglets were fed control diets with adequate methionine (NBW-CON), whereas 60 female IUGR piglets were fed either the control diets (IUGR-CON) or MR diets which were 30% reduced in methionine (IUGR-MR) (n=6 replicates (pens) with 5 piglets/replicate). At 49 and 105 days of age, one pig with a body weight near to the mean of each replication was selected for biochemical analysis. Read More

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December 2018
1 Read

Pulicaria crispa mitigates gastric ulcer induced by ethanol in rats: Role of treatment and auto healing.

Biomarkers 2018 Dec 4:1-32. Epub 2018 Dec 4.

d Clinical Pathology Laboratory , Cairo Specialized Hospital , Cairo , Egypt.

Context: Stomach ulcers are the common gastrointestinal disorders worldwide.

Objective: This study aimed to investigate the therapeutic impact of Pulicaria crispa aerial part ethanol extract against gastric ulcer in rats.

Materials And Methods: Ulcer was induced by one oral dose of ethanol (0. Read More

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December 2018
2 Reads

Effect of sodium-glucose cotransporter 2 inhibitor, empagliflozin, and α-glucosidase inhibitor, voglibose, on hepatic steatosis in an animal model of type 2 diabetes.

J Cell Biochem 2018 Nov 26. Epub 2018 Nov 26.

Division of Endocrinology and Metabolism, Department of Internal Medicine, St. Vincent's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Objective: We investigated the effects of sodium-glucose cotransporter 2 inhibitor, empagliflozin, and α-glucosidase inhibitor, voglibose, on hepatic steatosis in an animal model of type 2 diabetes (T2DM).

Methods: Empagliflozin (OLETF-EMPA) or voglibose (OLETF-VOG) was administered to Otsuka Long-Evans Tokushima fatty (OLETF) rats once daily for 12 weeks. Control Long-Evans Tokushima Otsuka (LETO) and OLETF (OLETF-C) rats received saline. Read More

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November 2018
3 Reads

Ameliorative potential of Cortex Lycii on enzymes involved in carbohydrate metabolism in streptozotocin-nicotinamide induced diabetic rats.

Pak J Pharm Sci 2018 Nov;31(6):2419-2428

Dongtai Institute of Tidal Flat, Nanjing Branch of Chinese Academy of Sciences, Dongtai, China.

Cortex Lycii (root back of Lycium chinense) has is a famous traditional Chinese medicine which displays several pharmacological activities including antioxidant and antidiabetic properties. We investigated the effect of the ethyl acetate fraction (QCL) of Cortex Lycii on the enzymes involved in the metabolism of carbohydrate in diabetic rat models. Streptozotocin-nicotinamide (110 and 65mg/kg body weight, respectively) was used to induce diabetes. Read More

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November 2018
5 Reads

Dietary administration of Bacillus amyloliquefaciens R8 reduces hepatic oxidative stress and enhances nutrient metabolism and immunity against Aeromonas hydrophila and Streptococcus agalactiae in zebrafish (Danio rerio).

Fish Shellfish Immunol 2018 Nov 21. Epub 2018 Nov 21.

Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung, 912, Taiwan; Research Center for Animal Biologics, National Pingtung University of Science and Technology, Pingtung, 912, Taiwan. Electronic address:

Zebrafish (Danio rerio) are an excellent model for assessing the beneficial effects of probiotics before applying them in aquaculture. This study evaluated the effects on zebrafish of dietary supplementation with the probiotic Bacillus amyloliquefaciens R8, which heterologously expresses xylanase from rumen fungi. Nutrient metabolism, hepatic oxidative stress, and innate immunity against pathogen infections were investigated. Read More

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November 2018
3 Reads

Hypoglycemic mechanisms of Ganoderma lucidum polysaccharides F31 in db/db mice via RNA-seq and iTRAQ.

Food Funct 2018 Nov 23. Epub 2018 Nov 23.

State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou 510070, China.

Our team has previously demonstrated that Ganoderma lucidum polysaccharides F31 have hypoglycemic effects on diabetic mice. This study provides insight into the system-level hypoglycemic mechanisms of F31 by the integrative analysis of transcriptomics and proteomics data. To explore the omics perspective for the mechanisms of action, the protein and gene expression in the liver from the normal control (NC), diabetic db/db control mice (DC) and F31-treated db/db mice (F31) were analyzed by iTRAQ and RNA-Seq. Read More

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November 2018
2 Reads

Scutellariae Radix and Coptidis Rhizoma Improve Glucose and Lipid Metabolism in T2DM Rats via Regulation of the Metabolic Profiling and MAPK/PI3K/Akt Signaling Pathway.

Int J Mol Sci 2018 Nov 18;19(11). Epub 2018 Nov 18.

Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, 138 Xianlin Road, Nanjing 210023, China.

Scutellariae Radix (SR) and Coptidis Rhizoma (CR) have often been combined to cure type 2 diabetes mellitus (T2DM) in the clinical practice for over thousands of years, but their compatibility mechanism is not clear. Mitogen-activated protein kinase (MAPK) signaling pathway has been suggested to play a critical role during the process of inflammation, insulin resistance, and T2DM. This study was designed to investigate their compatibility effects on T2DM rats and explore the underlying mechanisms by analyzing the metabolic profiling and MAPK/PI3K/Akt signaling pathway. Read More

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November 2018
6 Reads

Structural characterization, hypoglycemic effects and mechanism of a novel polysaccharide from Tetrastigma hemsleyanum Diels et Gilg.

Int J Biol Macromol 2018 Nov 14;123:775-783. Epub 2018 Nov 14.

School of Biotechnology and Health Science, Wuyi University, Jiangmen 529020, PR China; International Healthcare Innovation Institute (Jiangmen), Jiangmen 529040, PR China.

In the present study, a novel polysaccharide (THDP-3) purified from cane leaves of Tetrastigma hemsleyanum Diels et Gilg was obtained via anion exchange chromatography and the structural analyses of the compound indicated that THDP-3 is a homogenous polysaccharide with molecular weight of 77.98 kDa. Our results show that it consists of rhamnose, arabinose, mannose, glucose and galactose at molar ratio of 1. Read More

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November 2018
3 Reads

Effective Glucose Uptake by Human Astrocytes Requires Its Sequestration in the Endoplasmic Reticulum by Glucose-6-Phosphatase-β.

Curr Biol 2018 Nov 25;28(21):3481-3486.e4. Epub 2018 Oct 25.

Department of Pharmacology, University of Cambridge, Cambridge CB2 1PD, UK. Electronic address:

After its uptake into the cytosol, intracellular glucose is phosphorylated to glucose-6-phosphate (G6P), trapping it within the cell and preparing it for metabolism. In glucose-exporting tissues, like liver, G6P is transported into the ER, where it is dephosphorylated by G6Pase-α. The glucose is then returned to the cytosol for export [1, 2]. Read More

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November 2018
1 Read

Species-specific mechanisms of tumor suppression: trouble in paradigm.

Endocr Relat Cancer 2018 Oct 1. Epub 2018 Oct 1.

J Nyce, Clinical Research, ACGT Biotechnology, Collegeville, 19426-6219, United States.

A here-to-fore unknown tumor suppression mechanism reached its greatest expression in humans, having evolved to protect during the twenty-five-year lifespan of primitive humans. It depends upon high levels of circulating DHEAS and its importation into cells that have experienced inactivation of TP53, whereupon DHEAS is desulfated to DHEA, an uncompetitive inhibitor of Glucose-6-phosphate Dehydrogenase (G6PD). Uncompetitive inhibition of G6PD becomes irreversible in the presence of high levels of inhibitor and substrate (G6P). Read More

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October 2018
5 Reads

Involvement of AMPK activation in the inhibition of hepatic gluconeogenesis by Ficus carica leaf extract in diabetic mice and HepG2 cells.

Biomed Pharmacother 2018 Nov 2;109:188-194. Epub 2018 Nov 2.

Department of Pharmacy, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, 362000, People's Republic of China.

The aim of this study was to explore the possible mechanisms of Ficus carica leaf (FCL) extract in suppressing hepatic gluconeogenesis in diabetic mice. Diabetic mice (streptozotocin-induced) received 1 g/kg of FCL extract twice a day for 6 weeks. Fasting blood glucose levels were measured and a 2-h oral glucose tolerance test was conducted. Read More

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November 2018
3 Reads

IC-Tagging methodology applied to the expression of viral glycoproteins and the difficult-to-express membrane-bound IGRP autoantigen.

Sci Rep 2018 Nov 2;8(1):16286. Epub 2018 Nov 2.

Group of Molecular Virology, Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS), Departamento de Bioquímica e Bioloxía Molecular, Universidade de Santiago de Compostela, Santiago de Compostela, 15782, Spain.

We have previously developed a methodology to produce protein microspheres (MS) that can be loaded with proteins of interest in living cells through their C or N-terminal tagging with the so-called IC-Tag. The IC-Tagging method has many applications ranging from the production of immobilized enzymes for industrial use to the production of subunit vaccines due to its intrinsic adjuvancy. Here we show the adaptation of the IC-Tagging to work inside the endoplasmic reticulum and bacteria, allowing us to produce properly modified viral glycoproteins. Read More

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November 2018
6 Reads

Hexokinase-2 Expression in MET-positive FDG-negative Multiple Myeloma.

J Nucl Med 2018 Nov 2. Epub 2018 Nov 2.

University Hospital Würzburg, Germany.

Positron emission tomography (PET) with F-fluorodeoxyglucose (F-FDG) is the standard modality in nuclear medicine for imaging multiple myeloma (MM). However, viable MM as detected by magnetic resonance imaging or PET with other metabolic tracers including C-methionine (C-MET) may be missed by this technique, e.g. Read More

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November 2018
2 Reads

Functional polarization of human hepatoma HepaRG cells in response to forskolin.

Sci Rep 2018 Oct 31;8(1):16115. Epub 2018 Oct 31.

Univ Rennes, Inserm, EHESP, Irset (Institut de recherche en santé, environnement et travail) - UMR_S 1085, F-35000, Rennes, France.

HepaRG is an original human hepatoma cell line, acquiring highly differentiated hepatic features when exposed to dimethylsulfoxide (DMSO). To search alternatives to DMSO, which may exert some toxicity, we have analyzed the effects of forskolin (FSK), a cAMP-generating agent known to favor differentiation of various cell types. FSK used at 50 µM for 3 days was found to promote polarization of high density-plated HepaRG cells, i. Read More

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October 2018

Molecular and Metabolic Markers of Fructose Induced Hepatic Insulin Resistance in Developing and Adult Rats are Distinct and Aegle marmelos is an Effective Modulator.

Sci Rep 2018 Oct 29;8(1):15950. Epub 2018 Oct 29.

Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, Sec III, MB Road, New Delhi, 110017, India.

The time course of pathogenesis of fructose mediated hepatic insulin resistance (HepIR) is not well-delineated and we chronicle it here from post-weaning to adulthood stages. Weaned rats were provided for either 4 or 8 weeks, i.e. Read More

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October 2018
3 Reads

Molecular signaling mechanisms of renal gluconeogenesis in nondiabetic and diabetic conditions.

J Cell Physiol 2018 Oct 28. Epub 2018 Oct 28.

Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.

The kidneys are as involved as the liver in gluconeogenesis which can significantly contribute to hyperglycemia in the diabetic condition. Substantial evidence has demonstrated the overexpression of rate-limiting gluconeogenic enzymes, especially phosphoenolpyruvate carboxykinase and glucose 6 phosphatase, and the accelerated glucose release both in the isolated proximal tubular cells and in the kidneys of diabetic animal models and diabetic patients. The aim of this review is to provide an insight into the mechanisms that accelerate renal gluconeogenesis in the diabetic conditions and the therapeutic approaches that could affect this process in the kidney. Read More

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October 2018
1 Read

Bioactive Pentacyclic Triterpenes from the Root Bark Extract of Myrianthus arboreus, a Species Used Traditionally to Treat Type-2 Diabetes.

J Nat Prod 2018 Oct 18;81(10):2169-2176. Epub 2018 Oct 18.

Wood Chemistry Laboratory, Department of Wood Sciences , Université Laval , 1045 Québec G1 V 0A6 , Canada.

Four new Δ ursene-type pentacyclic triterpenes containing the trans-feruloyl moiety (1-4), along with ursolic acid (5), were isolated from a Myrianthus arboreus root bark ethanol extract, after bioassay-guided subfractionation of its hexane fraction. The structures of 1-4 were established on the basis of the results of standard spectroscopic analytical methods (IR, HRESIMS, GC-MS, 1D and 2D NMR). The compounds 3β- O- trans-feruloyl-2α,19α-dihydroxyurs-12-en-28-oic acid (1), 2α-acetoxy-3β- O- trans-feruloyl-19α-hydroxyurs-12-en-28-oic acid (3), and 5 were determined to decrease the activity of hepatocellular glucose-6-phosphatase (G6Pase) and to activate glycogen synthase (GS). Read More

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October 2018
11 Reads

Mogroside IIIE attenuates gestational diabetes mellitus through activating of AMPK signaling pathway in mice.

J Pharmacol Sci 2018 Sep 26. Epub 2018 Sep 26.

Heze Municipal Hospital, No. 2888, Caozhou Road, Mudan District, Heze City 274000, Shandong Province, China. Electronic address:

As one kind of complications of pregnancy, gestational diabetes mellitus (GDM) can influence the health of maternal-child in clinical practice. The C57 BL/KsJ(db/+) mice, genetic GDM model, and C57 BL/KsJ (wild-type) mice were purchased and classified into three groups: normal pregnancy (C57 BL/KsJ), GDM (C57 BL/KsJ), and GDM plus Mogroside IIIE (20.0 mg/kg) group. Read More

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September 2018
2 Reads

Hypothalamic PDE3B deficiency alters body weight and glucose homeostasis in mouse

J Endocrinol 2018 Oct 1;239(1):93-105. Epub 2018 Oct 1.

Department of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.

Pharmacological studies have suggested hypothalamic phosphodiesterase-3B to mediate leptin and insulin action in regulation of energy homeostasis. Whereas Pde3b-null mice show altered energy homeostasis, it is unknown whether this is due to ablation of Pde3b in the hypothalamus. Thus, to address the functional significance of hypothalamic phosphodiesterase-3B, we used Pde3bflox/flox and Nkx2. Read More

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October 2018

Detection of a novel, primate-specific ‘kill switch’ tumor suppression mechanism that may fundamentally control cancer risk in humans: an unexpected twist in the basic biology of TP53

Authors:
Jonathan W Nyce

Endocr Relat Cancer 2018 Nov 1;25(11):R497–R517. Epub 2018 Nov 1.

ACGT Biotechnology, Collegeville, Pennsylvania, USA

The activation of TP53 is well known to exert tumor suppressive effects. We have detected a primate-specific adrenal androgen-mediated tumor suppression system in which circulating DHEAS is converted to DHEA specifically in cells in which TP53 has been inactivated. DHEA is an uncompetitive inhibitor of glucose-6-phosphate dehydrogenase (G6PD), an enzyme indispensable for maintaining reactive oxygen species within limits survivable by the cell. Read More

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November 2018
10 Reads

Antidiabetic Activity of Triticum aestivum Seed-Based Diet on Alloxan-Induced Diabetic Rats.

J Diet Suppl 2018 Oct 4:1-17. Epub 2018 Oct 4.

a Department of Biochemistry, Faculty of Life Sciences , University of Ilorin , Nigeria.

The antidiabetic activity of Triticum aestivum seed-based diet on alloxan-induced diabetic rats was investigated. Forty-eight male and female albino rats of four groups were used for this study. Rats were sacrificed on day 28 and organs of interest were excised. Read More

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October 2018
2 Reads

Long-read sequencing identified a causal structural variant in an exome-negative case and enabled preimplantation genetic diagnosis.

Hereditas 2018 28;155:32. Epub 2018 Sep 28.

1Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, 410078 Hunan China.

Background: For a proportion of individuals judged clinically to have a recessive Mendelian disease, only one heterozygous pathogenic variant can be found from clinical whole exome sequencing (WES), posing a challenge to genetic diagnosis and genetic counseling. One possible reason is the limited ability to detect disease causal structural variants (SVs) from short reads sequencing technologies. Long reads sequencing can produce longer reads (typically 1000 bp or longer), therefore offering greatly improved ability to detect SVs that may be missed by short-read sequencing. Read More

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October 2018
3 Reads
1.120 Impact Factor

CREB-upregulated lncRNA MEG3 promotes hepatic gluconeogenesis by regulating miR-302a-3p-CRTC2 axis.

J Cell Biochem 2018 Sep 27. Epub 2018 Sep 27.

Department of Endocrinology, The First Affiliated Hospital of University of Science and Technology of China, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.

Hepatic gluconeogenesis is the major contributor to hyperglycemia in diabetes. Long noncoding RNA (lncRNA) maternally expressed gene 3 (MEG3) has been shown to promote hepatic insulin resistance; however, the underlying mechanism involving hepatic gluconeogenesis remains unclear. This study aims to investigate the potential role of MEG3 in hepatic gluconeogenesis. Read More

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September 2018
3.260 Impact Factor

Glucose 6 phosphatase dehydrogenase (G6PD) and neurodegenerative disorders: Mapping diagnostic and therapeutic opportunities.

Authors:
Manju Tiwari

Genes Dis 2017 Dec 23;4(4):196-203. Epub 2017 Sep 23.

Department of Biochemistry and Genetics, Barkatullah University, Bhopal, Madhya Pradesh, India.

Glucose 6 phosphate dehydrogenase (G6PD) is a key and rate limiting enzyme in the pentose phosphate pathway (PPP). The physiological significance of enzyme is providing reduced energy to specific cells like erythrocyte by maintaining co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). There are preponderance research findings that demonstrate the enzyme (G6PD) role in the energy balance, and it is associated with blood-related diseases and disorders, primarily the anemia resulted from G6PD deficiency. Read More

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December 2017
4 Reads

Bezafibrate induces autophagy and improves hepatic lipid metabolism in glycogen storage disease type Ia.

Hum Mol Genet 2018 Sep 25. Epub 2018 Sep 25.

Division of Medical Genetics, Department of Pediatrics, Duke University Medical Center, Durham, NC, USA.

Glucose-6-phosphatase α (G6Pase) deficiency, also known as von Gierke's Disease or GSD Ia, is characterized by decreased ability of the liver to convert glucose-6-phosphate (G6P) to glucose leading to glycogen accumulation and hepatosteatosis. Long-term complications of GSD Ia include hepatic adenomas and carcinomas, in association with the suppression of autophagy in the liver. The G6pc-/- mouse and canine models for GSD Ia were treated with the pan-peroxisomal proliferator-activated receptor (PPAR) agonist, bezafibrate, to determine the drug's effect on liver metabolism and function. Read More

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September 2018
7 Reads

Resveratrol Improves the Energy Sensing and Glycolipid Metabolism of Blunt Snout Bream Fed High-Carbohydrate Diets by Activating the AMPK-SIRT1-PGC-1α Network.

Front Physiol 2018 11;9:1258. Epub 2018 Sep 11.

Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.

This study investigated the effects of resveratrol on the growth performance, energy sensing, glycolipid metabolism and glucose and insulin load of blunt snout bream fed high-carbohydrate diets. Fish (39.44 ± 0. Read More

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September 2018
1 Read

SIRT1 activation attenuates α cell hyperplasia, hyperglucagonaemia and hyperglycaemia in STZ-diabetic mice.

Sci Rep 2018 Sep 18;8(1):13972. Epub 2018 Sep 18.

St. Michael's Hospital, Keenan Research Centre, Li Ka Shing Knowledge Institute, Toronto, M5B 1W8, Canada.

The NAD-dependent lysine deacetylase, Sirtuin 1 (SIRT1), plays a central role in metabolic regulation. With type 1 diabetes a disease that is characterised by metabolic dysregulation, we sought to assess the impact of SIRT1 activation in experimental, streptozotocin (STZ)-induced diabetes. CD1 mice with and without STZ-induced diabetes were randomized to receive the SIRT1 activating compound, SRT3025, or vehicle over 20 weeks. Read More

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September 2018
4 Reads

Tucum-do-cerrado (Bactris setosa Mart.) may enhance hepatic glucose response by suppressing gluconeogenesis and upregulating Slc2a2 via AMPK pathway, even in a moderate iron supplementation condition.

Food Res Int 2018 Nov 27;113:433-442. Epub 2018 Jul 27.

Postgraduate Program in Human Nutrition, Campus Universitário Darcy Ribeiro, Universidade de Brasília, Brasília, Distrito Federal, 70910-900, Brazil; Department of Nutrition, Campus Universitário Darcy Ribeiro, Universidade de Brasília, Brasília, Distrito Federal 70910-900, Brazil. Electronic address:

Dietary phytochemicals may improve glucose metabolism while iron excess seems to be associated to impaired glucose homeostasis and insulin responses. This study investigated the effect of tucum-do-cerrado (Bactris setosa Mart.) consumption on the carbohydrate metabolism and redox response in rats supplemented or not with dietary iron. Read More

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November 2018
6 Reads

Early-life exposure to gut microbiota from disease-protected mice does not impact disease outcome in type 1 diabetes susceptible NOD mice.

Immunol Cell Biol 2018 Sep 7. Epub 2018 Sep 7.

The University of Queensland Diamantina Institute, University of Queensland, Translational Research Institute, Brisbane, QLD, Australia.

The microbial community making up the gut microbiota can profoundly influence intestinal homeostasis and immune system development, and is believed to influence the development of complex diseases including type 1 diabetes (T1D). T1D susceptible nonobese diabetic (NOD) mice have been shown to harbor a distinct microbiota to disease-protected mice. We hypothesized that the T1D susceptible genetic background of NOD mice would be resistant to the introduction of a C57BL/6-derived microbiota. Read More

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September 2018

Glutathione S-transferase P Deficiency Induces Glucose Intolerance via JNK-dependent Enhancement of Hepatic Gluconeogenesis.

Am J Physiol Endocrinol Metab 2018 Aug 28. Epub 2018 Aug 28.

Diabetes and Obesity Center, University of Louisville, United States.

Hepatic glutathione S-transferases (GSTs) are dysregulated in human obesity, non-alcoholic fatty liver disease (NAFLD) and diabetes. The multifunctional GST Pi isoform (GSTP) catalyzes the conjugation of glutathione with acrolein and inhibits c-Jun NH2-terminal kinase (JNK) activation. Herein, we tested whether GSTP deficiency disturbs glucose homeostasis in mice. Read More

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August 2018
21 Reads

Intracellular lipids are an independent cause of liver injury and chronic kidney disease in non alcoholic fatty liver disease-like context.

Mol Metab 2018 Oct 1;16:100-115. Epub 2018 Aug 1.

Institut National de la Santé et de la Recherche Médicale, U1213, Lyon, F-69008, France; Université de Lyon, Lyon, F-69008, France; Université Lyon1, Villeurbanne, F-69622, France. Electronic address:

Objective: Ectopic lipid accumulation in the liver and kidneys is a hallmark of metabolic diseases leading to non-alcoholic fatty liver disease (NAFLD) and chronic kidney disease (CKD). Moreover, recent data have highlighted a strong correlation between NAFLD and CKD incidences. In this study, we use two mouse models of hepatic steatosis or CKD, each initiated independently of the other upon the suppression of glucose production specifically in the liver or kidneys, to elucidate the mechanisms underlying the development of CKD in the context of NAFLD-like pathology. Read More

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October 2018
1 Read

Metabolic Fate of the Glucose Taken up by the Intestine During Induced Hyperglycaemia in Dogs.

Niger J Physiol Sci 2018 Jun 30;33(1):37-49. Epub 2018 Jun 30.

Department of Physiology, College of Medicine, University of Ibadan, Ibadan.

Available data showed that the intestine increases it glucose uptake in response to hyperglycemia induced by anycause. However, what the intestine does with the glucose is not known. This study investigated the metabolic fate of theglucose taken up by the intestine during hyperglycaemia in dogs. Read More

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Conventional and Neo-antigenic Peptides Presented by β Cells Are Targeted by Circulating Naïve CD8+ T Cells in Type 1 Diabetic and Healthy Donors.

Cell Metab 2018 Dec 2;28(6):946-960.e6. Epub 2018 Aug 2.

INSERM, U1016, Cochin Institute, 75014 Paris, France; CNRS, UMR8104, Cochin Institute, 75014 Paris, France; Paris Descartes University, Sorbonne Paris Cité, 75014 Paris, France; Assistance Publique Hôpitaux de Paris, Service de Diabétologie, Cochin Hospital, 75014 Paris, France. Electronic address:

Although CD8 T-cell-mediated autoimmune β cell destruction occurs in type 1 diabetes (T1D), the target epitopes processed and presented by β cells are unknown. To identify them, we combined peptidomics and transcriptomics strategies. Inflammatory cytokines increased peptide presentation in vitro, paralleling upregulation of human leukocyte antigen (HLA) class I expression. Read More

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December 2018
13 Reads

Gene-gene interactions lead to higher risk for development of type 2 diabetes in a Chinese Han population: a prospective nested case-control study.

Lipids Health Dis 2018 Jul 28;17(1):179. Epub 2018 Jul 28.

Department of Epidemiology and Health Statistics, College of Public Health, Zhengzhou University, 100 Kexue Avenue, Zhengzhou, 450001, Henan, China.

Background: The purpose of this study was to evaluate the effect of single-nucleotide polymorphisms (SNPs) of the GCKR and G6PC2 genes on risk for type 2 diabetes and the SNP-SNP and haplotype-based interactions between these genes.

Methods: Subjects of this nested case-control study were selected from a prospective cohort residing in the rural area of Luoyang city in China. Cases (n = 538) were individually matched with controls. Read More

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July 2018
8 Reads
2.220 Impact Factor

Long-term complications of glycogen storage disease type Ia in the canine model treated with gene replacement therapy.

J Inherit Metab Dis 2018 Jul 24. Epub 2018 Jul 24.

Division of Medical Genetics, Duke University Medical Center (DUMC), Box 103856, Durham, NC, 27710, USA.

Background: Glycogen storage disease type Ia (GSD Ia) in dogs closely resembles human GSD Ia. Untreated patients with GSD Ia develop complications associated with glucose-6-phosphatase (G6Pase) deficiency. Survival of human patients on intensive nutritional management has improved; however, long-term complications persist including renal failure, nephrolithiasis, hepatocellular adenomas (HCA), and a high risk for hepatocellular carcinoma (HCC). Read More

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July 2018
9 Reads

Potential activities and mechanisms of extracellular polysaccharopeptides from fermented Trametes versicolor on regulating glucose homeostasis in insulin-resistant HepG2 cells.

PLoS One 2018 19;13(7):e0201131. Epub 2018 Jul 19.

Department of Nutritional Science, Fu Jen Catholic University, New Taipei City, Taiwan.

Polysaccharides derived from mushrooms have potential to control blood sugar, reduce insulin resistance and prevent diabetic complications. The intracellular polysaccharopeptides of Trametes versicolor (TV) have been used as immunologic and oncologic adjuvants. The aim of this study was to investigate the potential activities and mechanisms of extracellular polysaccharopeptides (ePSP) obtained from TV strain LH-1 on regulating glucose homeostasis. Read More

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July 2018
1 Read

Species-specific low plasma glucose in fish is associated with relatively high tissue glucose content and is inversely correlated with cardiac glycogen content.

J Comp Physiol B 2018 Jul 14. Epub 2018 Jul 14.

Department of Ocean Sciences, Ocean Sciences Centre, Memorial University of Newfoundland, St. John's, NL, A1C 5S7, Canada.

The relationship between plasma glucose concentration and intracellular glucose (liver, heart, brain, gill, gonad, intestine, kidney, spleen, white muscle) was determined in fish species with a range in plasma glucose (Atlantic cod, 5.06 mM; cunner, 3.8 mM; rainbow trout, 3. Read More

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Histochemistry, immunohistochemistry and cytochemistry of the anterior midgut region of the stingless bee Melipona quadrifasciata and honey bee Apis mellifera (Hymenoptera: Apidae).

Micron 2018 Oct 28;113:41-47. Epub 2018 Jun 28.

Departamento de Biologia Geral, Universidade Federal de Viçosa, 36570-900 Viçosa, Minas Gerais, Brazil. Electronic address:

The anterior midgut region of stingless bees is anatomically differentiated with tall and narrow cells, whereas in other social and solitary bees this anatomical gut region is lacking. The objective of the present study was to describe the histochemistry, immunohistochemistry and cytochemistry of the anterior midgut region of the stingless bee Melipona quadrifasciata in comparison with the honey bee Apis mellifera. The anterior midgut region of both species was evaluated for identification of the enzymes β- galactosidase, glucose-6-phosphatase, acid phosphatase, and alkaline phosphatase, the membrane transporter aquaporin, the hormone FMRF-amide, and lysosomes. Read More

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October 2018
2 Reads

Linked Hexokinase and Glucose-6-Phosphatase Activities Reflect Grade of Ovarian Malignancy.

Mol Imaging Biol 2018 Jul 9. Epub 2018 Jul 9.

Department of Nuclear Medicine, Odense University Hospital, Kløvervænget 47, 5000, Odense, Denmark.

Purpose: Malignant cells exhibit increased rates of aerobic glycolysis. Here, we tested whether the accumulation of fluoro-deoxyglucose-6-phosphate (FDG6P) in ovarian cancers of differential malignancy reflects inversely correlated elevations of hexokinase (HK) and glucose-6-phosphatase (G6Pase) activities.

Procedures: Twenty-nine women with suspected ovarian cancer had positron emission tomography (PET) prior to surgery. Read More

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July 2018
1 Read

The cardenolides strophanthidin, digoxigenin and dihydroouabain act as activators of the human RORγ/RORγT receptors.

Toxicol Lett 2018 Oct 5;295:314-324. Epub 2018 Jul 5.

Laboratory of Transcriptional Regulation, Institute of Medical Biology, Polish Academy of Sciences, 93-232 Lodz, Poland. Electronic address:

Two isoforms of a ligand-activated nuclear receptor, RORγ and RORγT, have been implicated in various physiological functions, including energy metabolism, circadian rhythm and immune system development. Using a stably transfected reporter cell line, we screened two chemical libraries and identified three cardenolides (natural, plant-derived pesticides) as activators of RORγ-dependent transcription. These compounds increased G6PC and NPAS2 expression in HepG2 cells, accompanied by increased occupancy of RORγ within the promoters of these genes. Read More

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October 2018
7 Reads

Possible ameliorative effect of aqueous extract of date (Phoenix dactylifera) pits in rats exposed to gamma radiation.

Int J Radiat Biol 2018 Sep 10;94(9):815-824. Epub 2018 Aug 10.

a Radiation Biology Department , National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority , Cairo , Egypt.

Purpose: To evaluate the radioprotective efficacy of date (Phoenix dactylifera) pits extract (DPE) against γ-irradiation-induced liver damage in the male albino rats. For this study, 30-day survival, dose reduction factor (DRF), antioxidant status and some biochemical changes in the serum and hepatic tissue were evaluated.

Materials And Methods: To calculate 30-day survival, median lethal dose of 30 days (LD50/30) and DRF, DPE was orally administered to rats for 21 days before irradiation with different doses (5, 6, 8, 10 and 12 Gy) of γ-rays and the rats were observed for 30 days post-irradiation. Read More

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September 2018

Red Pepper (Capsicum annuum L.) Seed Extract Decreased Hepatic Gluconeogenesis and Increased Muscle Glucose Uptake In Vitro.

J Med Food 2018 Jul 3;21(7):665-671. Epub 2018 Jul 3.

1 Department of Food and Nutrition, Hanyang University , Seoul, Korea.

Red pepper seed, a by-product of red pepper, has been reported to have antioxidant and antiobesity activities. However, its role in diabetes has not yet been highly investigated. Glucose homeostasis is mainly maintained by insulin, which suppresses glucose production in the liver and enhances glucose uptake in peripheral tissues. Read More

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July 2018
9 Reads

The role of kidney in the inter-organ coordination of endogenous glucose production during fasting.

Mol Metab 2018 Oct 18;16:203-212. Epub 2018 Jun 18.

Institut National de la Santé et de la Recherche Médicale, U1213, Lyon, F-69008, France; Université de Lyon, Lyon, F-69008, France; Université Lyon1, Villeurbanne, F-69622, France. Electronic address:

Objective: The respective contributions to endogenous glucose production (EGP) of the liver, kidney and intestine vary during fasting. We previously reported that the deficiency in either hepatic or intestinal gluconeogenesis modulates the repartition of EGP via glucagon secretion (humoral factor) and gut-brain-liver axis (neural factor), respectively. Considering renal gluconeogenesis reportedly accounted for approximately 50% of EGP during fasting, we examined whether a reduction in renal gluconeogenesis could promote alterations in the repartition of EGP in this situation. Read More

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October 2018
4 Reads

The SMILE transcriptional corepressor inhibits cAMP response element-binding protein (CREB)-mediated transactivation of gluconeogenic genes.

J Biol Chem 2018 Aug 27;293(34):13125-13133. Epub 2018 Jun 27.

From the National Creative Research Initiatives Center for Nuclear Receptor Signals and Hormone Research Center, School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea,

Under fasting conditions, activation of several hepatic genes sets the stage for gluconeogenesis in the liver. cAMP response element-binding protein (CREB), CREB-regulated transcription coactivator 2 (CRTC2), and peroxisome proliferator-activated receptor γ coactivator 1-alpha (PGC-1α) are essential for this transcriptional induction of gluconeogenic genes. PGC-1α induction is mediated by activation of a CREB/CRTC2 signaling complex, and recent findings have revealed that small heterodimer partner-interacting leucine zipper protein (SMILE), a member of the CREB/ATF family of basic region-leucine zipper (bZIP) transcription factors, is an insulin-inducible corepressor that decreases PGC-1α expression and abrogates its stimulatory effect on hepatic gluconeogenesis. Read More

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August 2018
1 Read

Detection of a novel, primate-specific 'kill switch' tumor suppression mechanism that may fundamentally control cancer risk in humans: an unexpected twist in the basic biology of TP53.

Authors:
Jonathan W Nyce

Endocr Relat Cancer 2018 Nov 25;25(11):R497-R517. Epub 2018 Jun 25.

ACGT BiotechnologyCollegeville, Pennsylvania, USA

The activation of TP53 is well known to exert tumor suppressive effects. We have detected a adrenal androgen-mediated tumor suppression system in which circulating DHEAS is converted to DHEA specifically in cells in which TP53 has been DHEA is an inhibitor of glucose-6-phosphate dehydrogenase (G6PD), an enzyme indispensable for maintaining reactive oxygen species within limits survivable by the cell. Uncompetitive inhibition is otherwise unknown in natural systems because it becomes in the presence of high concentrations of substrate and inhibitor. Read More

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November 2018
4 Reads

Dendrobium mixture regulates hepatic gluconeogenesis in diabetic rats via the phosphoinositide-3-kinase/protein kinase B signaling pathway.

Exp Ther Med 2018 Jul 18;16(1):204-212. Epub 2018 May 18.

Institute of Integrated Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.

The present study aimed to evaluate the impact of dendrobium mixture (DMix) on the gene and protein expression of insulin signaling pathway-associated factors in the livers of diabetic rats. The molecular mechanisms by which DMix inhibits gluconeogenesis were also investigated. A total of 47 female Wistar rats were used in the present study. Read More

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July 2018
1 Read

Kaempferol ameliorates hyperglycemia through suppressing hepatic gluconeogenesis and enhancing hepatic insulin sensitivity in diet-induced obese mice.

J Nutr Biochem 2018 Aug 1;58:90-101. Epub 2018 May 1.

Department of Human Nutrition, Foods and Exercise, College of Agricultural and Life Sciences, Virginia Tech, Blacksburg, VA, USA. Electronic address:

Obesity-associated insulin resistance (IR) is a major risk factor for developing type 2 diabetes and an array of other metabolic disorders. In particular, hepatic IR contributes to the increase in hepatic glucose production and consequently the development of fasting hyperglycemia. In this study, we explored whether kaempferol, a flavonoid isolated from Gink go biloba, is able to regulate hepatic gluconeogenesis and blood glucose homeostasis in high-fat diet-fed obese mice and further explored the underlying mechanism by which it elicits such effects. Read More

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August 2018
10 Reads

Effect of magnesium sulfate administration to improve insulin resistance in type 2 diabetes animal model: using the hyperinsulinemic-euglycemic clamp technique.

Fundam Clin Pharmacol 2018 Dec 4;32(6):603-616. Epub 2018 Jul 4.

Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

This study attempted to elucidate the possible mechanism of magnesium sulfate (MgSO ) administration on reducing insulin resistance in type 2 diabetic rats. Fifty Wistar rats were divided into five groups: NDC was fed the normal diet, CD received high-fat diet with 35 mg/kg of streptozotocin, CD-Mg animals received MgSO via drinking water, CD-Ins1, and CD-Ins2 animals treated with low or high dose of insulin. Body weight and blood glucose levels were measured weekly. Read More

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December 2018
24 Reads

Polycystic kidney features of the renal pathology in glycogen storage disease type I: possible evolution to renal neoplasia.

J Inherit Metab Dis 2018 Jun 4. Epub 2018 Jun 4.

Institut National de la Santé et de la Recherche by Inserm, U1213, 69008, Lyon, France.

Glycogen storage disease type I (GSDI) is a rare genetic pathology characterized by glucose-6 phosphatase (G6Pase) deficiency, translating in hypoglycemia during short fasts. Besides metabolic perturbations, GSDI patients develop long-term complications, especially chronic kidney disease (CKD). In GSDI patients, CKD is characterized by an accumulation of glycogen and lipids in kidneys, leading to a gradual decline in renal function. Read More

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June 2018
11 Reads