20,812 results match your criteria Germinal Matrix Hemorrhage Imaging


DNA damage and repair in the female germline: contributions to ART.

Hum Reprod Update 2018 Dec 12. Epub 2018 Dec 12.

Priority Research Centre for Reproductive Science, Discipline of Biological Sciences, University of Newcastle, Callaghan, NSW, Australia.

Background: DNA integrity and stability are critical determinants of cell viability. This is especially true in the female germline, wherein DNA integrity underpins successful conception, embryonic development, pregnancy and the production of healthy offspring. However, DNA is not inert; rather, it is subject to assault from various environment factors resulting in chemical modification and/or strand breakage. Read More

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December 2018

Dasatinib is preferentially active in the activated B-cell subtype of diffuse large B-cell lymphoma.

J Proteome Res 2018 Dec 12. Epub 2018 Dec 12.

Diffuse large B-cell lymphoma (DLBCL) is a heterogeneous disease, with at least one-third of its patients relapsing after treatment with the current chemotherapy regimen, R-CHOP. By gene expression profiling, patients with DLBCL can be categorized into two clinically relevant subtypes: activated B-cell (ABC) and germinal center B-cell (GCB) DLBCL. Patients with the ABC subtype have a poorer prognosis than those with GCB, and are defined by chronic, overactive signaling through the B-cell receptor and NF-κB pathways. Read More

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December 2018

Do immature and mature sibling oocytes recovered from stimulated cycles have the same reproductive potential?

Reprod Biomed Online 2018 Dec 6;37(6):667-676. Epub 2018 Oct 6.

IVF Laboratory, IVIRMA-Valencia, Valencia 46015, Spain; IVI Foundation, INCLIVA, Valencia 46026, Spain. Electronic address:

Research Question: How can laboratory and clinical outcomes of spontaneously, early maturing germinal-vesicle oocytes and sibling in-vivo-matured (metaphase II [MII]) oocytes be quantified and compared?

Design: A prospective, non-randomized intra-cohort study of oocytes from women aged 38 years or younger, with six or fewer MII oocytes and four or more germinal vesicles retrieved. No indication was identified for genetic tests or oocyte or embryo cryopreservation. The study was carried out at IVIRMA-Valencia. Read More

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December 2018

Artificial Neural Network to Predict Varicocele Impact on Male Fertility through Testicular Endocannabinoid Gene Expression Profiles.

Biomed Res Int 2018 13;2018:3591086. Epub 2018 Nov 13.

Faculty of Biosciences and Technology for Food, Agriculture and Environment, University of Teramo, 64100 Teramo, Italy.

The relationship between varicocele and fertility has always been a matter of debate because of the absence of predictive clinical indicators or molecular markers able to define the severity of this disease. Even though accumulated evidence demonstrated that the endocannabinoid system (ECS) plays a central role in male reproductive biology, particularly in the testicular compartment, to date no data point to a role for ECS in the etiopathogenesis of varicocele. Therefore, the present research has been designed to investigate the relationship between testicular ECS gene expression and fertility, using a validated animal model of experimental varicocele (VAR), taking advantage of traditional statistical approaches and artificial neural network (ANN). Read More

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November 2018
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Histone demethylase LSD1 is required for germinal center formation and BCL6-driven lymphomagenesis.

Nat Immunol 2019 Jan 10;20(1):86-96. Epub 2018 Dec 10.

Department of Medicine, Division of Hematology & Medical Oncology, Weill Cornell Medicine, New York, NY, USA.

Germinal center (GC) B cells feature repression of many gene enhancers to establish their characteristic transcriptome. Here we show that conditional deletion of Lsd1 in GCs significantly impaired GC formation, associated with failure to repress immune synapse genes linked to GC exit, which are also direct targets of the transcriptional repressor BCL6. We found that BCL6 directly binds LSD1 and recruits it primarily to intergenic and intronic enhancers. Read More

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January 2019

Cumulus-oocyte interactions and programmed cell death in bovine embryos produced in vitro.

Theriogenology 2018 Nov 26;126:81-87. Epub 2018 Nov 26.

Laboratory of Molecular Biology, Faculty of Animal Science, University of Sao Paulo, São Paulo, Brazil.

This study investigated the interactions between cumulus cells (CCs) and oocytes and programmed cell death in bovine cumulus-oocyte complexes (COCs) with different morphological characteristics. DNA fragmentation was assessed in CCs at 0 and 24 h of maturation, as well as parthenogenetic developmental competence on the 9th day post-activation, blastocyst quality and BCL-2 and BAX transcript levels in matured CCs. Most immature oocytes in the COC-A group (full cumulus and several compact layers) were in the initial germinal vesicle (iGV) stage, exhibiting minimal or no DNA damage. Read More

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November 2018

Multi-type Galton-Watson Processes with Affinity-Dependent Selection Applied to Antibody Affinity Maturation.

Bull Math Biol 2018 Dec 7. Epub 2018 Dec 7.

Département d'Informatique, Ecole Normale Supérieure, 45 rue d'Ulm, 75005, Paris, France.

We analyze the interactions between division, mutation and selection in a simplified evolutionary model, assuming that the population observed can be classified into fitness levels. The construction of our mathematical framework is motivated by the modeling of antibody affinity maturation of B-cells in germinal centers during an immune response. This is a key process in adaptive immunity leading to the production of high-affinity antibodies against a presented antigen. Read More

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December 2018

Effect of Vitamin E in Prevention of Intraventricular Hemorrhage in Preterm Neonates.

Int J Prev Med 2018 5;9:97. Epub 2018 Nov 5.

Department of Pediatrics, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Background: Germinal matrix-intraventricular hemorrhage (IVH) is the most common brain hemorrhage in preterm neonates. The importance of this hemorrhage lies in its severe complications. There is no definite treatment for IVH in neonates; therefore, the prevention of IVH should be considered. Read More

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November 2018

Identification of CVID Patients With Defects in Immune Repertoire Formation or Specification.

Front Immunol 2018 23;9:2545. Epub 2018 Nov 23.

Department of Immunology, Erasmus MC University Medical Center, Rotterdam, Netherlands.

Common variable immune deficiency disorder (CVID) is the most clinically relevant cause of antibody failure. It is a highly heterogeneous disease with different underlying etiologies. CVID has been associated with a quantitative B cell defect, however, little is known about the quality of B cells present. Read More

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November 2018

Utility of Various Ultrafast Magnetic Resonance Sequences in the Detection of Fetal Intracranial Hemorrhage.

Ann Indian Acad Neurol 2018 Oct-Dec;21(4):275-279

Department of Sonography, Mediscan Systems, Chennai, Tamil Nadu, India.

Objective: The aim of this study is to compare the images obtained from standard ultrafast magnetic resonance (MR) imaging sequences with gradient (GRE) sequence images in identifying fetal intracranial hemorrhage (ICH).

Materials And Methods: MR images of fetal brains with ICH done between October 2012 and September 2015 were reviewed. The images obtained from four ultrafast MR sequences- Turbo Fast Low Angle Shot (Turbo FLASH) T1-weighted images, Half Fourier Acquisition single-shot turbo spin echo (HASTE) T2-weighted images, b0 images of diffusion-weighted imaging (DWI) and b800 images of DWI were compared with images obtained from GRE sequence in depicting fetal ICH. Read More

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December 2018
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Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches.

J Vis Exp 2018 Nov 21(141). Epub 2018 Nov 21.

Division of Hematology-Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School; Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School;

In the gut mucosa, immune cells constitute a unique immunological entity, which promotes immune tolerance while concurrently conferring immune defense against pathogens. It is well established that Peyer's patches (PPs) have an essential role in the mucosal immune network by hosting several effector T and B cell subsets. A certain fraction of these effector cells, follicular T helper (TFH) and germinal center (GC) B cells are professionalized in the regulation of humoral immunity. Read More

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November 2018

Pravastatin attenuates testicular damage induced by doxorubicin - a stereological and histopatological study.

J Basic Clin Physiol Pharmacol 2018 Dec 11. Epub 2018 Dec 11.

Cellular and Molecular Research Center, Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Background The aim of this study is to investigate the effects of pravastatin (PS) against doxorubicin (DOX)-induced testicular toxicity. Methods A total of 24 healthy male Sprague-Dawley rats were equally divided into four groups. Group I received normal saline, Group II received PS (20 mg/kg b. Read More

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December 2018

Germinal center humoral autoimmunity independently mediates progression of allograft vasculopathy.

J Autoimmun 2018 Dec 6. Epub 2018 Dec 6.

University of Cambridge, School of Clinical Medicine, Cambridge, CB2 0QQ, UK. Electronic address:

The development of humoral autoimmunity following organ transplantation is increasingly recognised, but of uncertain significance. We examine whether autoimmunity contributes independently to allograft rejection. In a MHC class II-mismatched murine model of chronic humoral rejection, we report that effector antinuclear autoantibody responses were initiated upon graft-versus-host allorecognition of recipient B cells by donor CD4 T-cells transferred within heart allografts. Read More

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December 2018

Lack of NWC protein (c11orf74 homolog) in murine spermatogenesis results in reduced sperm competitiveness and impaired ability to fertilize egg cells in vitro.

PLoS One 2018 6;13(12):e0208649. Epub 2018 Dec 6.

Laboratory of Molecular and Cellular Immunology, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.

NWC is an uncharacterised protein containing three strongly conserved domains not found in any other known protein. Previously, we reported that the NWC protein is detected in cells in the germinal layer in murine testes (strain: C57BL/6), and its knockout results in no obvious phenotype. We determined the NWC expression pattern during spermatogenesis, and found this protein in spermatocytes and round spermatids, but not in epididymal sperm. Read More

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December 2018

Comparison of GnRH agonist and hCG for priming in vitro maturation cycles in cancer patients undergoing urgent fertility preservation.

PLoS One 2018 6;13(12):e0208576. Epub 2018 Dec 6.

Department of Reproductive Medicine & Fertility Preservation, Hôpital Jean Verdier, Bondy, France.

In vitro maturation (IVM) of oocytes retrieved at germinal vesicle or Metaphase I stage, followed by vitrification of Metaphase II (MII) oocytes, has recently emerged as an option for urgent fertility preservation (FP). Priming is usually achieved with an injection of hCG, 10,000 IU, 36 hours before retrieval. This study aimed to assess a new method of priming, using GnRH agonists, and compare it to hCG, in cancer patients undergoing urgent FP. Read More

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December 2018

Assessing the role of the T-box transcription factor Eomes in B cell differentiation during either Th1 or Th2 cell-biased responses.

PLoS One 2018 6;13(12):e0208343. Epub 2018 Dec 6.

Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia.

Successful T-dependent humoral responses require the production of antibody-secreting plasmablasts, as well as the formation of germinal centers which eventually form high-affinity B cell memory. The ability of B cells to differentiate into germinal center and plasma cells, as well as the ability to tailor responses to different pathogens, is driven by transcription factors. In T cells, the T-box transcription factors T-bet and Eomesodermin (Eomes) regulate effector and memory T cell differentiation, respectively. Read More

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December 2018

Loss of ARHGEF1 causes a human primary antibody deficiency.

J Clin Invest 2018 Dec 6. Epub 2018 Dec 6.

ARHGEF1 is a RhoA-specific guanine nucleotide exchange factor expressed in hematopoietic cells. We used whole-exome sequencing to identify compound heterozygous mutations in ARHGEF1, resulting in the loss of ARHGEF1 protein expression in two primary-antibody-deficient siblings presenting with recurrent severe respiratory tract infections and bronchiectasis. Both ARHGEF1-deficient patients showed an abnormal B cell immunophenotype, with a deficiency in marginal-zone and memory B cells and an increased frequency of transitional B cells. Read More

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December 2018

Follicular Development and Reproductive Characteristics in Four Species of Brazilian Tropidurus Lizards.

Zoolog Sci 2018 Dec;35(6):553-563

4 Biology Institute, State University of Campinas. Monteiro Lobato st., 255 - Cidade Universitária, Campinas, São Paulo 13083-862, Brazil.

The follicular development and reproductive characteristics of four species of oviparous lizards in the Tropidurus torquatus group were anatomically and histologically evaluated. We measured specimens, recorded the number of follicles and eggs, and removed the right ovary of each individual, which we processed according to histological routine and photo-documented. For all species, ovaries were divided into a cortical germinal bed, where oogonia and stage I oocytes are located, and a medullar stroma, where the remaining follicular developmental stages occur. Read More

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December 2018

Ly9 (CD229) Antibody Targeting Depletes Marginal Zone and Germinal Center B Cells in Lymphoid Tissues and Reduces Salivary Gland Inflammation in a Mouse Model of Sjögren's Syndrome.

Front Immunol 2018 16;9:2661. Epub 2018 Nov 16.

Immunology Unit, Department of Biomedical Sciences, Medical School, University of Barcelona, Barcelona, Spain.

Sjögren's Syndrome (SjS) is a common chronic autoimmune disease characterized by the B cell hyperactivation, lymphocyte infiltration, and tissue damage of exocrine glands. It can also present life-threatening extraglandular manifestations, such as pulmonary and hepatic involvement, renal inflammation and marginal zone (MZ) B cell lymphoma. Several biologic agents have been tested in SjS but none has shown significant efficacy. Read More

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November 2018
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Robust adaptive immune response against infection marked by low parasitemia in a murine model of sickle cell disease.

Blood Adv 2018 Dec;2(23):3462-3478

Laboratory of Complement Biology and.

The intraerythrocytic parasite is the number 1 cause of transfusion-transmitted infection and can induce serious, often life-threatening complications in immunocompromised individuals including transfusion-dependent patients with sickle cell disease (SCD). Despite the existence of strong long-lasting immunological protection against a second infection in mouse models, little is known about the cell types or the kinetics of protective adaptive immunity mounted following infection, especially in infection-prone SCD that are thought to have an impaired immune system. Here, we show, using a mouse infection model, that infected wild-type (WT) mice mount a very strong adaptive immune response, characterized by (1) coordinated induction of a robust germinal center (GC) reaction; (2) development of follicular helper T (T) cells that comprise ∼30% of splenic CD4 T cells at peak expansion by 10 days postinfection; and (3) high levels of effector T-cell cytokines, including interleukin 21 and interferon γ, with an increase in the secretion of antigen (Ag)-specific antibodies (Abs). Read More

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December 2018

Long-Lived Plasma Cells in Mice and Men.

Front Immunol 2018 16;9:2673. Epub 2018 Nov 16.

Department of Rheumatology and Inflammation Research, Institute of Medicine, University of Gothenburg, Gothenburg, Sweden.

Even though more than 30 years have passed since the eradication of smallpox, high titers of smallpox-specific antibodies are still detected in the blood of subjects vaccinated in childhood. In fact, smallpox-specific antibody levels are maintained in serum for more than 70 years. The generation of life-long immunity against infectious diseases such as smallpox and measles has been thoroughly documented. Read More

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November 2018

Adipose tissue: A natural resource for multipotent mesenchymal stem cells with potential translation to trigerminal layers.

Indian J Plast Surg 2018 May-Aug;51(2):177-181

Manipal Institute of Regenerative Medicine, Bengaluru, Karnataka, India.

Background: The article reports basic science research that establishes that adipose tissue (AT)-derived mesenchymal stem cells (MSCs) have a potential to transgerminal translation.

Study Design: MSC confirmation was obtained by phenotypic spindle-shaped cells as well as with four positive and three negative markers. The translineage translation of adipose-derived MSCs (ADMSCs) was established. Read More

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December 2018

Intrinsic properties of human germinal center B cells set antigen affinity thresholds.

Sci Immunol 2018 Nov;3(29)

Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA.

Protective antibody responses to vaccination or infection depend on affinity maturation, a process by which high-affinity germinal center (GC) B cells are selected on the basis of their ability to bind, gather, and present antigen to T follicular helper (T) cells. Here, we show that human GC B cells have intrinsically higher-affinity thresholds for both B cell antigen receptor (BCR) signaling and antigen gathering as compared with naïve B cells and that these functions are mediated by distinct cellular structures and pathways that ultimately lead to antigen affinity- and T cell-dependent differentiation to plasma cells. GC B cells bound antigen through highly dynamic, actin- and ezrin-rich pod-like structures that concentrated BCRs. Read More

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November 2018
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Immunolocalization of RFamide-related peptide 3 in a desert rodent Gerbillus tarabuli during seminiferous epithelium cycle.

Tissue Cell 2018 Dec 20;55:1-12. Epub 2018 Sep 20.

USTHB, University of Sciences and Technology of Houari Boumediene, Biological Sciences Faculty, Aride Area Research Laboratory, Algiers, Algeria.

Gerbillus tarabuli is a nocturnal seasonal breeder desert rodent with a main breeding season in spring and summer, and sexual quiescence in winter. This species is an interesting model for studying testis function in rodents. Therefore, the present study was performed firstly to investigate the stages of seminiferous epithelium cycle of Gerbillus tarabuli with a histological, morphometric and statistical study. Read More

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December 2018

Detection of intracellular Helicobacter pylori in Candida. SPP from neonate oral swabs.

Rev Assoc Med Bras (1992) 2018 Oct;64(10):928-935

Universidad de Concepción, Víctor Lamas 1290, casilla 160 C, Concepción, Región del Biobío, Chile.

Background: There is evidence of detection of Helicobacter pylori (H. pylori) in the stool of newborns and in the yeast that colonizes the oral cavity of this age group. However, there is a lack of research to confirm it. Read More

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October 2018
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Adenosine 2a receptor signals block autoimmune arthritis by inhibiting pathogenic germinal center T follicular helper cells.

Arthritis Rheumatol 2018 Dec 5. Epub 2018 Dec 5.

Division of Rheumatic and Autoimmune Diseases, Department of Medicine and Center for Immunology, University of Minnesota Medical School, Minneapolis, MN, USA.

Objective: CD4 germinal center (GC)-T follicular helper (Tfh) cells are important in the pathogenesis of autoimmune arthritis. Previous studies have shown that adenosine 2a receptor (A2aR, Adora2a) signaling can divert CD4 T cells away from the GC-Tfh cell lineage during the primary response to foreign antigens. Therefore, we examined the effects of A2aR signaling on CD4 T cells during the recognition of a self-antigen using a mouse model of autoimmune arthritis. Read More

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December 2018

Clinicopathological and immunological features of follicular pancreatitis-a distinct disease entity characterized by Th17 activation.

Histopathology 2018 Dec 4. Epub 2018 Dec 4.

Department of Pathology and Laboratory Medicine, Kansai Medical University, Osaka, Japan.

Aim: Follicular pancreatitis is a recently recognized, distinct clinicopathological entity characterized by the presence of many intrapancreatic lymphoid follicles with reactive germinal centres. However, the clinicopathological and immunological features and causes have not yet been established. We assessed the clinicopathological and immunological profiles of patients with follicular pancreatitis who underwent surgery. Read More

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December 2018

Dysregulated miR-155 and miR-125b Are Related to Impaired B-cell Responses in Down Syndrome.

Front Immunol 2018 20;9:2683. Epub 2018 Nov 20.

B cell Pathophysiology Unit, Immunology Research Area, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.

Children with Down Syndrome (DS) suffer from immune deficiency with a severe reduction in switched memory B cells (MBCs) and poor response to vaccination. Chromosome 21 (HSA21) encodes two microRNAs (miRs), miR-125b, and miR-155, that regulate B-cell responses. We studied B- and T- cell subpopulations in tonsils of DS and age-matched healthy donors (HD) and found that the germinal center (GC) reaction was impaired in DS. Read More

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November 2018
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Antigen Specificity and Clinical Significance of IgG and IgA Autoantibodies Produced by Tumor-Infiltrating B Cells in Breast Cancer.

Front Immunol 2018 20;9:2660. Epub 2018 Nov 20.

Cancer Biomarker and Immunotherapy Group, Latvian Biomedical Research and Study Centre, Riga, Latvia.

An important role for tumor infiltrating B lymphocytes (TIL-B) in the immune response to cancer is emerging; however, very little is known about the antigen specificity of antibodies produced . The presence of IgA antibodies in the tumor microenvironment has been noted although their biological functions and clinical significance are unknown. This study used a 91-antigen microarray to examine the IgG and IgA autoantibody repertoires in breast cancer (BC). Read More

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November 2018
2 Reads

Contactin-1/F3 Regulates Neuronal Migration and Morphogenesis Through Modulating RhoA Activity.

Front Mol Neurosci 2018 20;11:422. Epub 2018 Nov 20.

Institute of Brain Science, National Yang-Ming University, Taipei, Taiwan.

During neocortical development, newborn neurons migrate along radial fibers from the germinal ventricular zone (VZ) toward the cortical plate (CP) to populate the cerebral cortex. This radial migration requires adhesion activities between neurons and radial fibers; however, past research has identified only a limited number of adhesion molecules involved in this process. Contactin-1/F3 (Cntn1), a cell adhesion molecule expressed in the developing nervous system is essential for many key developmental events including neural cell adhesion, neurite outgrowth, axon guidance and myelination. Read More

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November 2018

TET2 Deficiency Sets the Stage for B-cell Lymphoma.

Cancer Discov 2018 Dec;8(12):1515-1517

Cancer Genetics and Genomics Program, Duke Cancer Institute, Center for Genomic and Computational Biology, Duke University, Durham, North Carolina.

is a well-established tumor suppressor in the context of myeloid malignancies, but its role in lymphoma development has been less clear. In this issue of , Dominguez and colleagues report that TET2 function is critical for germinal center exit and plasma cell differentiation, and its deficiency can lead to B-cell lymphoma phenotypes.. Read More

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December 2018
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Effects of Acute Fluorene-9-Bisphenol Exposure on Mouse Oocyte in vitro Maturation and Its Possible Mechanisms.

Environ Mol Mutagen 2018 Nov 30. Epub 2018 Nov 30.

Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China.

Fluorene-9-bisphenol (BHPF), a substitute of bisphenol A (BPA) used in the production of the so-called "BPA-free" plastics, has now been shown to be released from commercial plastic bottles into drinking water and has strong anti-estrogenic activity in mice, which suggests that BHPF is also an environmental toxin. However, whether BHPF exposure has effects on mouse oocyte development is unknown. In this study, the influence of acute exposure to BHPF (50-150 μM, 12 hr) on mouse oocyte maturation and its possible mechanisms were investigated. Read More

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November 2018

The carotid body: a physiologically relevant germinal niche in the adult peripheral nervous system.

Cell Mol Life Sci 2018 Nov 29. Epub 2018 Nov 29.

Instituto de Biomedicina de Sevilla (IBiS), Laboratory 103, Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Dpto. de Fisiología Médica y Biofísica, Avda, Manuel Siurot, s/n., 41013, Sevilla, Spain.

Oxygen constitutes a vital element for the survival of every single cell in multicellular aerobic organisms like mammals. A complex homeostatic oxygen-sensing system has evolved in these organisms, including detectors and effectors, to guarantee a proper supply of the element to every cell. The carotid body represents the most important peripheral arterial chemoreceptor organ in mammals and informs about hypoxemic situations to the effectors at the brainstem cardiorespiratory centers. Read More

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November 2018

p53 expression in large B-cell lymphomas with MYC extra copies and CD99 expression in large B-cell lymphomas in relation to MYC status.

Hum Pathol 2018 Nov 26. Epub 2018 Nov 26.

Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USA. Electronic address:

p53 expression and MYC-extra copies (MYC-EC) have been reported to serve as independent adverse prognostic markers in patients with diffuse large B-cell lymphoma (DLBCL). However, the impact of p53 expression in MYC-EC lymphomas has not been delineated. Conversely, CD99 expression has been shown to have a positive impact on survival in patients with germinal center-type DLBCL, yet nothing is reported about the impact of CD99 expression and MYC status. Read More

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November 2018

Aluminum granuloma in a child secondary to DTaP-IPV vaccination: A case report.

Pediatr Dermatol 2018 Nov 28. Epub 2018 Nov 28.

Department of Dermatology, Oregon Health & Science University, Portland, Oregon.

Reports detailing the acute formation of aluminum granulomas, which can cause persistent, intensely pruritic nodules secondary to the administration of aluminum-containing vaccines, are infrequently described in medical literature. To our knowledge, this is the first report describing the development of an aluminum granuloma causing a persistent, pruritic nodule at the injection site following the administration of the DTaP-IPV vaccine. We present the case of a 6-year-old girl who developed a severely pruritic subcutaneous nodule on her anterior right thigh at the injection site three weeks after the administration of the aluminum-containing DTaP-IPV (Kinrix) vaccine. Read More

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November 2018
6 Reads

Effect of Neoadjuvant and Adjuvant Therapy of Experimental Breast Cancer on the Structure of Mesenteric Lymph Nodes.

Bull Exp Biol Med 2018 Dec 28;166(2):245-249. Epub 2018 Nov 28.

Research Institute of Experimental and Clinical Lymphology, Affiliated Branch of Federal Research Center of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.

The morphometric analysis of mesenteric lymph nodes was carried out in female Wistar rats with chemically induced breast cancer. In control rats with untreated breast cancer, the volume of the system of sinuses increased in parallel with the appearance of morphological signs of suppression of cell-mediated immunity, inhibition of humoral immunity, and macrophage reaction. Against the background of chemotherapy, we observed a decrease in the volume of paracortex and lymphoid nodules, suppression of proliferative activity of lymphoid cells in paracortical and B-cell zone, and a decrease in macrophage content. Read More

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December 2018
1 Read

Single-cell RNA sequencing unveils an IL-10-producing helper subset that sustains humoral immunity during persistent infection.

Nat Commun 2018 11 28;9(1):5037. Epub 2018 Nov 28.

Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, WI, 53213, USA.

During chronic viral infection, the inflammatory function of CD4 T-cells becomes gradually attenuated. Concurrently, Th1 cells progressively acquire the capacity to secrete the cytokine IL-10, a potent suppressor of antiviral T cell responses. To determine the transcriptional changes that underlie this adaption process, we applied a single-cell RNA-sequencing approach and assessed the heterogeneity of IL-10-expressing CD4 T-cells during chronic infection. Read More

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November 2018
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Single-Cell Transcriptomics Characterizes Cell Types in the Subventricular Zone and Uncovers Molecular Defects Impairing Adult Neurogenesis.

Cell Rep 2018 Nov;25(9):2457-2469.e8

Systems Biology of Gene Regulatory Elements, Berlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine in the Helmholtz Association, 13092 Berlin-Buch, Germany. Electronic address:

Neural stem cells (NSCs) contribute to plasticity and repair of the adult brain. Niches harboring NSCs regulate stem cell self-renewal and differentiation. We used comprehensive and untargeted single-cell RNA profiling to generate a molecular cell atlas of the largest germinal region of the adult mouse brain, the subventricular zone (SVZ). Read More

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November 2018
4 Reads

Stereological and morphological analysis of the effects of aging on spermatogenesis in rat testis 1.

Acta Cir Bras 2018 Oct;33(10):904-913

PhD, Associate Professor, Department of Urology, School of Medicine, UFPR, Curitiba-PR, Brazil. Scientific, intellectual, conception and design of the study; analysis and interpretation of data; statistical analysis; critical revision.

Purpose: To quantify, through stereological and morphometric analysis, spermatogenesis in rats undergoing the natural aging process.

Methods: Seventy-two male Wistar rats were divided into 6 equal groups according to age at the time of killing: 3, 6, 9, 12, 18, and 24 months. All the rats were subjected orchiectomy and collection of testicular parenchymal fragments for histological and morphometric analysis. Read More

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October 2018

A multi-gene panel beyond BRCA1/BRCA2 to identify new breast cancer-predisposing mutations by a picodroplet PCR followed by a next-generation sequencing strategy: a pilot study.

Anal Chim Acta 2019 Jan 18;1046:154-162. Epub 2018 Sep 18.

CEINGE-Biotecnologie Avanzate, via Gaetano Salvatore 486, 80145, Naples, Italy; Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, via Sergio Pansini 5, 80131, Naples, Italy. Electronic address:

By analyzing multiple gene panels, next-generation sequencing is more effective than conventional procedures in identifying disease-related mutations that are useful for clinical decision-making. Here, we aimed to test the efficacy of an 84 genes customized-panel in BRCA1 and BRCA2 mutation-negative patients. Twenty-four patients were enrolled in this study. Read More

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January 2019

In vitro maturation in subfertile women with polycystic ovarian syndrome undergoing assisted reproduction.

Cochrane Database Syst Rev 2018 11 15;11:CD006606. Epub 2018 Nov 15.

Assisted Reproduction Unit, 3rd Department of Obstetrics and Gynaecology, Medical School, National and Kapodistrian University of Athens, Attikon University Hospital, Rimini 1, Athens, Chaidari, Greece, 12462.

Background: Polycystic ovarian syndrome (PCOS) occurs in 4% to 7% of all women of reproductive age and 50% of women presenting with subfertility. Subfertility affects 15% to 20% of couples trying to conceive. A significant proportion of these women ultimately need assisted reproductive technology (ART). Read More

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November 2018
3 Reads

Effector B cells in cardiac allograft vasculopathy.

Authors:
Emmanuel Zorn

Curr Opin Organ Transplant 2018 Nov 21. Epub 2018 Nov 21.

Columbia Center for Translational Immunology, New York Presbyterian Hospital, Columbia University Medical Center, New York, New York, USA.

Purpose Of Review: B cells have recently emerged as important immune players in solid organ rejection, especially in cardiac allograft vasculopathy (CAV), a chronic form of rejection following heart transplantation. B cells can exert either regulatory or effector functions. This review will provide an update on effector B cells in CAV. Read More

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November 2018
6 Reads

Testicular structure and spermatogenesis of the oviparous goodeids Crenichthys baileyi (Gilbert, 1893) and Empetrichthys latos Miller, 1948 (Teleostei, Cyprinodontiformes).

J Morphol 2018 Dec 26;279(12):1787-1797. Epub 2018 Nov 26.

Division of Fishes, Department of Vertebrate Zoology, National Museum of Natural History, Smithsonian Institution, Washington, P.O. Box 37012, MRC 159, District of Columbia.

The cyprinodontiform family Goodeidae comprises some 51 species, including subspecies, of freshwater fishes all of which are at risk or are extinct in the wild. It is classified in two allopatric subfamilies: the Goodeinae, endemic to the Mexican Plateau, and the Empetrichthyinae, known only from relict taxa in Nevada and southern California. The 41 species of goodeins are all viviparous and share a set of well-documented reproductive characters. Read More

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December 2018

A CD4 T cell population expanded in lupus blood provides B cell help through interleukin-10 and succinate.

Nat Med 2018 Nov 26. Epub 2018 Nov 26.

Baylor Institute for Immunology Research, Dallas, TX, USA.

Understanding the mechanisms underlying autoantibody development will accelerate therapeutic target identification in autoimmune diseases such as systemic lupus erythematosus (SLE). Follicular helper T cells (T cells) have long been implicated in SLE pathogenesis. Yet a fraction of autoantibodies in individuals with SLE are unmutated, supporting that autoreactive B cells also differentiate outside germinal centers. Read More

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November 2018
1 Read

Hippo Pathway Kinase Mst1 Is Required for Long-Lived Humoral Immunity.

J Immunol 2018 Nov 26. Epub 2018 Nov 26.

Institut de Recherches Cliniques de Montréal, Montreal, Quebec H2W 1R7, Canada;

The protein kinase Mst1 is a key component of the evolutionarily conserved Hippo pathway that regulates cell survival, proliferation, differentiation, and migration. In humans, Mst1 deficiency causes primary immunodeficiency. Patients with MST1-null mutations show progressive loss of naive T cells but, paradoxically, mildly elevated serum Ab titers. Read More

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November 2018
4 Reads

Human SPG11 cerebral organoids reveal cortical neurogenesis impairment.

Hum Mol Genet 2018 Nov 22. Epub 2018 Nov 22.

Department of Stem Cell Biology (former IZKF junior research group III).

SPG11 linked hereditary spastic paraplegia is a complex monogenic neurodegenerative disease that in addition to spastic paraplegia is characterized by childhood onset cognitive impairment, thin corpus callosum and enlarged ventricles. We have previously shown impaired proliferation of SPG11 neural progenitor cells (NPCs). For delineation of potential defect in SPG11 brain development we employ 2D culture systems and 3D human brain organoids derived from SPG11 patients' iPSC and controls. Read More

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November 2018

Valosin-containing protein is associated with maintenance of meiotic arrest in mouse oocytes.

Biol Reprod 2018 Nov 21. Epub 2018 Nov 21.

College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, P. R. China.

Valosin-containing protein (VCP) is a member of the highly conserved AAA (ATPase associated with a variety of cellular activities) superfamily. A previous study has shown that targeted deletion of Vcp in mice results in early embryonic lethality. The aim of the present study was to analyze the expression and localization of VCP and its function in meiotic arrest of mouse oocytes. Read More

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November 2018
3 Reads

TACI Contributes to Host Resistance by Controlling T Follicular Helper Cell Response and Germinal Center Formation.

Front Immunol 2018 9;9:2612. Epub 2018 Nov 9.

US Food and Drug Administration, Division of Bacterial Allergenic and Parasitic Diseases, Center for Biologics Evaluation and Research, Silver Spring, MD, United States.

The delay in parasite-specific B cell development leaves people in malaria endemic areas vulnerable to repeated infections. Here, we investigated the role of transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI), a molecule involved in the generation of antigen-specific antibody secreting cells, in host response to non-lethal infection. We found that TACI deficiency not only resulted in higher peak parasitemia levels in challenged mice, but also led to a delay in parasite clearance and anti- Merozoite Surface Protein 1(C-terminal 19-kDa fragment [rMSP-1]) protein and anti-rMSP-1 and anti- IgG antibody development. Read More

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November 2018
2 Reads

Melatonin-induced demethylation of antioxidant genes increases antioxidant capacity through RORαin cumulus cells of prepubertal lambs.

Free Radic Biol Med 2018 Nov 22. Epub 2018 Nov 22.

Animal Husbandry and Veterinary Research Institute of Tianjin, Tianjin 300412, China. Electronic address:

Physical damage and oxidative stress may occur in prepubertal cumulus cells, due to insufficient glutathione synthesis. To determine potential epigenetic mechanisms related to antioxidant effects of melatonin on ovine prepubertal cumulus cells, 30 lambs, 4-wk-old were randomly allocated into two groups: a control (C, n=20) group and a melatonin (M, n=10) group given a subcutaneous implant containing 18mg melatonin. All lambs were superovulated (250 IU FSH and 250 IU eCG). Read More

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November 2018

Vaccine nanoparticles displaying recombinant Ebola virus glycoprotein for induction of potent antibody and polyfunctional T cell responses.

Nanomedicine 2018 Nov 22. Epub 2018 Nov 22.

Department of Pharmaceutical Sciences, University of Michigan, Ann Arbor, MI, USA; Biointerfaces Institute, University of Michigan, Ann Arbor, MI, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA. Electronic address:

The recent outbreaks of Ebolavirus (EBOV) in West Africa underscore the urgent need to develop an effective EBOV vaccine. Here, we report the development of synthetic nanoparticles as a safe and highly immunogenic platform for vaccination against EBOV. We show that a large recombinant EBOV antigen (rGP) can be incorporated in a configurational manner into lipid-based nanoparticles, termed interbilayer-crosslinked multilamellar vesicles (ICMVs). Read More

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November 2018
5 Reads