1,563,947 results match your criteria Genetic testing and molecular biomarkers[Journal]
J Vet Sci 2018 Dec 4. Epub 2018 Dec 4.
Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Seoul, 05030, Korea.
Genome-wide association study (GWAS) is a powerful tool for identifying the genetic causes of various diseases. This study conducted to identify genomic variation in Maltese dog genomes associated with degenerative mitral valve disease (DMVD) development and evaluate the association of each biological condition with DMVD in Maltese dogs. DNA was extracted from blood samples obtained from 48 Maltese dogs (32 with DMVD and 16 controls). Read More
Tierarztl Prax Ausg K Kleintiere Heimtiere 2018 Oct 12;46(5):289-295. Epub 2018 Dec 12.
Objective: Transitional cell carcinoma (TCC) is the most common malignant tumour of the canine urinary tract. Previously, the mutation of the BRAF gene V595E was identified in approximately 85 % of canine TCC cases by DNA sequencing of TCC tumour cells, both in frozen and paraffin-embedded tissue sections, as well as in urine. The objective of this study was to establish these methods in cytological smears and to investigate the prevalence of BRAF mutation V595E in canine TCC in our cohort of patients. Read More
Neuropediatrics 2018 Dec 12. Epub 2018 Dec 12.
Department of Medical Genetics, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
X-linked myotubular myopathy (XLMTM) is a rare inherited neuromuscular disorder associated with mutations in the gene on the Xq28 region. We report a severely affected girl with XLMTM, caused by maternally inherited 661 kb Xq28 microduplication identified by chromosomal microarray analysis and confirmed also on DNA from muscle biopsy with a custom-designed X-chromosome-specific microarray. X-inactivation analysis revealed a skewed inactivation pattern on the proband's muscle biopsy. Read More
Nucleic Acids Res 2018 Dec 12. Epub 2018 Dec 12.
Molecular Biology Division, Biomedical Center, Faculty of Medicine and Center for Integrated Protein Science Munich (CIPSM), Ludwig-Maximilians-Universität Munich, 82151 Martinsried, Germany.
Transcription regulators select their genomic binding sites from a large pool of similar, non-functional sequences. Although general principles that allow such discrimination are known, the complexity of DNA elements often precludes a prediction of functional sites. The process of dosage compensation in Drosophila allows exploring the rules underlying binding site selectivity. Read More
ILAR J 2018 Dec 12. Epub 2018 Dec 12.
Translational Medicine and The Comparative Medicine Institute, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina.
For more than a century, transplantation of tissues and organs from animals into man, xenotransplantation, has been viewed as a potential way to treat disease. Ironically, interest in xenotransplantation was fueled especially by successful application of allotransplantation, that is, transplantation of human tissue and organs, as a treatment for a variety of diseases, especially organ failure because scarcity of human tissues limited allotransplantation to a fraction of those who could benefit. In principle, use of animals such as pigs as a source of transplants would allow transplantation to exert a vastly greater impact than allotransplantation on medicine and public health. Read More
Sleep 2018 Dec 12. Epub 2018 Dec 12.
Department of Pathology and Laboratory Medicine, Children's Hospital Los Angeles, University of Southern California, Los Angeles, CA.
Sleep abnormalities are common among children with neurodevelopmental disorders. The human chr16p11.2 microdeletion is associated with a range of neurological and neurobehavioral abnormalities. Read More
J Infect Dis 2018 Dec 12. Epub 2018 Dec 12.
Radboud university medical center, Department of Internal Medicine and Radboud Center of Infectious Diseases, Nijmegen, the Netherlands.
Background: Rewiring cellular metabolism is important for activation of immune cells during host defense against Mycobacterium tuberculosis. Glutamine has been implicated as an immunomodulatory nutrient, but its role in response to M. tuberculosis is unknown. Read More
J Hered 2018 Dec 12. Epub 2018 Dec 12.
School of Life Sciences, Arizona State University, Tempe, AZ.
The organisms of the Galapagos Islands played a central role in the development of the theory of evolution by Charles Darwin. Examination of the population genetics factors of many of these organisms with modern molecular methods have expanded our understanding of their evolution. Here I provide a perspective on how selection, gene flow, genetic drift, mutation, and inbreeding have contributed to the evolution of six of the iconic Galapagos species; flightless cormorant, pink iguana, marine iguana, Galapagos hawk, giant tortoises, and Darwin's finches. Read More
J Biochem 2018 Dec 12. Epub 2018 Dec 12.
Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, Japan.
Males and females share the same genetic code, but gene expression profile often displays differences between two sexes. Mouse embryonic fibroblasts (MEFs) have been used to experiment as a useful tool to test gene function. They have also been characterized by gender-based differences in expressed genes such as Y-linked Sry or X-linked Hprt. Read More
Endocr Rev 2018 Dec 12. Epub 2018 Dec 12.
Bioinformatics 2018 Dec 12. Epub 2018 Dec 12.
Department of Biosystems Science and Engineering, ETH Zurich, Basel, 4058, Switzerland SIB Swiss Institute of Bioinformatics.
Summary: Combinatorial association mapping aims to assess the statistical association of higher-order interactions between genetic markers with a phenotype of interest. This paper presents CASMAP, a software package that leverages recent advances in significant pattern mining to overcome the statistical and computational challenges that have hindered combinatorial association mapping. CASMAP can be used to perform region-based association studies and to detect higher-order epistatic interactions of genetic variants. Read More
J Natl Cancer Inst 2018 Dec 12. Epub 2018 Dec 12.
Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD.
Background: Genome-wide association studies (GWAS) identify associations of individual single-nucleotide polymorphisms (SNPs) with cancer risk but usually only explain a fraction of the inherited variability. Pathway analysis of genetic variants is a powerful tool to identify networks of susceptibility genes.
Methods: We conducted a large agnostic pathway-based meta-analysis of GWAS data using the summary-based adaptive rank truncated product method to identify gene sets and pathways associated with pancreatic ductal adenocarcinoma (PDAC) in 9040 cases and 12 496 controls. Read More
Jpn J Clin Oncol 2018 Dec 12. Epub 2018 Dec 12.
Department of Clinical Genetics, Yokohama City University Hospital, Yokohama, Japan.
Background: Application of next-generation DNA sequencing (NGS) has recently become increasingly common in the field of clinical oncology in several countries around the world. In Japan also, a system for applying NGS to routine clinical practice is gradually being established. During this process, we introduced in Japan the tumor-profiling MSK-IMPACT (Memorial Sloan Kettering-Integrated Mutation Profiling of Actionable Cancer Targets) assay. Read More
Hum Reprod Update 2018 Dec 12. Epub 2018 Dec 12.
Priority Research Centre for Reproductive Science, Discipline of Biological Sciences, University of Newcastle, Callaghan, NSW, Australia.
Background: DNA integrity and stability are critical determinants of cell viability. This is especially true in the female germline, wherein DNA integrity underpins successful conception, embryonic development, pregnancy and the production of healthy offspring. However, DNA is not inert; rather, it is subject to assault from various environment factors resulting in chemical modification and/or strand breakage. Read More
Int J Epidemiol 2018 Dec 11. Epub 2018 Dec 11.
Population Health Sciences, University of Bristol, Bristol, UK.
Background: Accumulating evidence suggests that breastfeeding benefits children's intelligence, possibly due to long-chain polyunsaturated fatty acids (LC-PUFAs) present in breast milk. Under a nutritional adequacy hypothesis, an interaction between breastfeeding and genetic variants associated with endogenous LC-PUFAs synthesis might be expected. However, the literature on this topic is controversial. Read More
Hum Mol Genet 2018 Dec 12. Epub 2018 Dec 12.
KU Leuven - Department of Neurosciences, Laboratory for Neuroimmunology, B-3000 Leuven, Belgium.
The role of somatic variants in diseases beyond cancer is increasingly being recognized, with potential roles in autoinflammatory and autoimmune diseases. However, as mutation rates and allele fractions are lower, studies in these diseases are substantially less tolerant of false positives and bio-informatics algorithms require high replication rates. We developed a pipeline combining two variant callers, MuTect2 and VarScan2, with technical filtering and prioritization. Read More
Gerontology 2018 Dec 12:1-9. Epub 2018 Dec 12.
Alzheimer's disease (AD) affects nearly 50 million people worldwide, and currently no disease-modifying treatment is available. With continuous failure of anti-amyloid-beta- or tau-based therapies, identification of new targets has become an urgent necessity for AD prevention and therapy. Recently, conventional genetic approaches and computational strategies have converged on immune-inflammatory pathways as key events in the pathogenesis of AD. Read More
Nano Lett 2018 Dec 12. Epub 2018 Dec 12.
Controlling the nonlinear optical response of nanoscale metamaterials opens new exciting applications such as frequency conversion or flat metal optical elements. To utilize the already well-developed fabrication methods, a systematic design methodology for obtaining high nonlinearities is required. In this paper we consider an optimization-based approach, combining a multiparameter genetic algorithm with 3D Finite-Difference Time Domain simulations. Read More
Ear Nose Throat J 2018 Dec;97(12):396-402
College of Medicine, Chang Gung University, Taoyuan, Taiwan.
The etiology of Ménière disease (MD) is multifactorial; genetic factors seem to play an important role. The associations between MD and human leukocyte antigen (HLA) status have been studied previously in several populations and have shown that the HLA alleles imparting susceptibility varied. In the present study, we explored HLA status in Taiwanese patients with definitive MD. Read More
Magy Onkol 2018 Dec 30;62(4):214-221. Epub 2018 Oct 30.
Patológiai Intézet, PTE Klinikai Központ, Pécs, Hungary.
Acute lymphoblastic leukemia (ALL) is the most frequent malignancy in children. In Hungary 60-70 new cases are diagnosed annually. The survival rate is 85-90% in developed countries with current treatment protocols. Read More
PLoS One 2018 12;13(12):e0208560. Epub 2018 Dec 12.
Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI, United States of America.
Using time-series transcriptomic data from poplar roots undergoing polyethylene glycol (PEG)-induced drought stress, we built a genetic network model of the involved putative molecular responses. We found that the network resembled a hierarchical structure. The highest hierarchical level in this structure is occupied by 9 genes, which we called superhubs because they were primarily connected to 18 hub genes, which are then connected to 2,934 terminal genes. Read More
PLoS One 2018 12;13(12):e0208862. Epub 2018 Dec 12.
Department of Plant Pathology, University of California-Davis, Davis, California, United States of America.
Grapevine leafroll-associated virus 3 (GLRaV-3) is the most widely prevalent and economically important of the complex of RNA viruses associated with grapevine leafroll disease (GLD). Phylogenetic studies have grouped GLRaV-3 isolates into nine different monophyletic groups and four supergroups, making GLRaV-3 a genetically highly diverse virus species. In addition, new divergent variants have been discovered recently around the world. Read More
PLoS One 2018 12;13(12):e0209034. Epub 2018 Dec 12.
Division of Experimental Hematology and Cancer Biology, Cancer and Blood Disease Institute, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, United States of America.
Genetic manipulation of NOD/SCID (NS) mice has yielded numerous sub-strains with specific traits useful for the study of human hematopoietic xenografts, each with unique characteristics. Here, we have compared the engraftment and output of umbilical cord blood (UCB) CD34+ cells in four immune-deficient strains: NS, NS with additional IL2RG knockout (NSG), NS with transgenic expression of human myeloid promoting cytokines SCF, GM-CSF, and IL-3 (NSS), and NS with both IL2RG knockout and transgenic cytokine expression (NSGS). Overall engraftment of human hematopoietic cells was highest in the IL2RG knockout strains (NSG and NSGS), while myeloid cell output was notably enhanced in the two strains with transgenic cytokine expression (NSS and NSGS). Read More
PLoS One 2018 12;13(12):e0208920. Epub 2018 Dec 12.
Department of Clinical Haematology, Oxford University Hospitals NHS Foundation Trust, Oxford, United Kingdom.
Bortezomib, a first generation proteasome inhibitor, is used in both newly diagnosed and relapsed myeloma settings. Considerable differences exist in the usage of bortezomib therapy in the clinical practice setting in comparison to clinical trial setting as well manufacturer's recommendations. These differences include route of administration (intravenous (iv) vs. Read More
PLoS One 2018 12;13(12):e0208771. Epub 2018 Dec 12.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China) in Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou, China.
Litchi (Litchi chinesis Sonn.) is the most economically significant member of Sapindaceae family, especially in sub-tropical regions. However, its tall tree body often brings many inconveniences to production management. Read More
PLoS One 2018 12;13(12):e0209026. Epub 2018 Dec 12.
Department of Pediatrics, National Jewish Health, Denver, CO, United States of America.
Cystic fibrosis (CF) is the most common life-shortening genetic disease and is caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Several current therapies aim at improving availability and/or function of the mutant CFTR proteins. The combination therapeutic lumacaftor/ivacaftor (Orkambi, luma/iva) partially corrects folding and potentiates CFTR function impaired by the F508del mutation. Read More
PLoS One 2018 12;13(12):e0208085. Epub 2018 Dec 12.
Department for Companion Animals and Horses, University Clinic for Small Animals, Small Animal Surgery, Section for Physical Therapy, University of Veterinary Medicine, Veterinärplatz, Vienna, Austria.
Current research on gait analysis mostly involves horses and dogs. Feline kinetics and kinematics are being investigated and receiving more clinical interest at present. Ground reaction forces measured on pressure-sensitive mattresses have been established in healthy Domestic Shorthair cats (DSH). Read More
PLoS One 2018 12;13(12):e0207936. Epub 2018 Dec 12.
Department of Biology, Stanford University, Stanford, California, United States of America.
Species are shifting their ranges due to climate change, many moving to cooler and higher locations. However, with elevation increase comes oxygen decline, potentially limiting a species' ability to track its environment depending on what mechanisms it has available to compensate for hypoxic stress. Pikas (Family Ochotonidae), cold-specialist small mammal species, are already undergoing elevational range shifts. Read More
PLoS One 2018 12;13(12):e0207429. Epub 2018 Dec 12.
Bureau of Nutritional Sciences, Health Products and Food Branch, Health Canada, Ottawa, ON, Canada.
The US Institute of Medicine defined serum 25-hydroxyvitamin D (25OHD) cut point values of 30 nmol/L and 40 nmol/L were used to assess the vitamin D status of South Asian and European Canadians of self-identified ancestry living in the National Capital Region of Canada. Serum 25OHD values were measured in the spring and fall of 2012 to represent status during the winter and summer months, respectively. A total of 1238 measurements were obtained from 669 participants (49% South Asian ancestry): some participants were measured only once (spring or fall). Read More
PLoS One 2018 12;13(12):e0207480. Epub 2018 Dec 12.
Federal Center for Animal Health, Vladimir, Russia.
Wide spread incidences of vaccine-like strains of lumpy skin disease virus (LSDV) have recently been reported in a Russian region with a neighboring country that actively vaccinate with a live attenuated LSD vaccine. The use of live-attenuated viruses (LAVs) as vaccines during an active outbreak, creates potential ground for coinfection of hosts and emergence of a strain combining genetic fragments of both parental vaccine and field strains. In this study, we analyse the vaccine-like strain LSDV RUSSIA/Saratov/2017 detected in Saratovskaya oblast, a region sharing border with Kazakhstan. Read More
PLoS Genet 2018 Dec 12;14(12):e1007702. Epub 2018 Dec 12.
Department of the Control of Genetic Processes, Institute of Gene Biology Russian Academy of Sciences, Moscow, Russia.
Drosophila bithorax complex (BX-C) is one of the best model systems for studying the role of boundaries (insulators) in gene regulation. Expression of three homeotic genes, Ubx, abd-A, and Abd-B, is orchestrated by nine parasegment-specific regulatory domains. These domains are flanked by boundary elements, which function to block crosstalk between adjacent domains, ensuring that they can act autonomously. Read More
PLoS One 2018 12;13(12):e0207140. Epub 2018 Dec 12.
R&D Alternative Discovery and Development, GlaxoSmithKline, Stevenage, Hertfordshire, United Kingdom.
Atonal homolog 1 (Atoh1) is a basic helix-loop-helix 9 (bHLH) transcription factor acting downstream of Notch and is required for the differentiation of sensory hair cells in the inner ear and the specification of secretory cells during the intestinal crypt cell regeneration. Motivated by the observations that the upregulation of Atoh1 gene expression, through genetic manipulation or pharmacological inhibition of Notch signaling (e.g. Read More
Rev Gastroenterol Peru 2018 Jul-Sep;38(3):265-279
Laboratorio de Genética Humana, Universidad Nacional Mayor de San Marcos. Lima, Perú; Laboratorio de Biología Molecular, Laboratorio Clínico Roe. Lima, Perú.
This review aims to present the genetic, clinical and diagnostic aspects of Lynch syndrome, as well as providing the most relevant information about genetic counseling in these patients and the current recommendations for their surveillance. Read More
Am J Surg Pathol 2018 Dec 11. Epub 2018 Dec 11.
INSERM1052, CNRS 5286, Lyon-Sud Charles Mérieux Lyon-1 Faculty, Claude Bernard University.
Gray-zone lymphoma (GZL) with features intermediate between classic Hodgkin lymphoma (cHL) and large B-cell lymphoma (LBCL) was introduced as a provisional entity into the World Health Organization classification in 2008. However, as diagnostic criteria are imprecise, reliable identification of GZL cases remains challenging. Here, we describe the histopathologic features of 139 GZL cases from a retrospective Lymphoma Study Association (LYSA) study with the goal to improve classification accuracy. Read More
Phytopathology 2018 Dec 12. Epub 2018 Dec 12.
Rutgers The State University of New Jersey, 242612, Department of Plant Biology, New Brunswick, New Jersey, United States ;
Anisogramma anomala (Peck) E. Müller, a biotrophic ascomycete in the order Diaporthales, causes eastern filbert blight (EFB) of hazelnuts (Corylus). Until recently, little has been documented on its genetic diversity and population structure. Read More
Vector Borne Zoonotic Dis 2018 Dec 12. Epub 2018 Dec 12.
1 Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University , Changsha, People's Republic of China .
Toxoplasma gondii is a ubiquitous apicomplexan parasite of warm-blooded animals and humans. However, limited information is available about T. gondii infection in wild birds. Read More
Plant Dis 2018 Dec 12:PDIS01180147RE. Epub 2018 Dec 12.
Department of Plant Pathology and Weed Research, Agricultural Research Organization (ARO), the Volcani Center, Rishon Le'Zion, 7528809 Israel.
Heart rot, caused by Alternaria alternata, is a major pomegranate disease that impacts production worldwide; most fruits in orchards are colonized by A. alternata but, nevertheless, symptoms are apparent on only a small proportion of the colonized fruits. During the years of our previous research it was noticed that within individual orchards, the incidence of pomegranate fruits exhibiting heart rot symptoms was related to the visual appearance of the trees: trees that appeared visually frail bore more diseased fruits than robust trees. Read More
Mol Biol Cell 2018 Dec 12:mbcE18060345. Epub 2018 Dec 12.
Department of Molecular Biology and Biochemistry, Centre for Cell Biology, Development and Disease, Simon Fraser University, Burnaby, V5A 1S6, Canada.
Actomyosin contractility can influence the canonical Wnt signaling pathway in processes like mesoderm differentiation and tissue stiffness during tumorigenesis. We identified that increased non-muscle myosin II activation and cellular contraction inhibited Wnt target gene transcription in developing Drosophila imaginal discs. Genetic interactions studies were used to show that this effect was due to myosin-induced accumulation of cortical F-actin resulting in clustering and accumulation of E-cadherin to the adherens junctions. Read More
Plant Signal Behav 2018 Dec 12:1-6. Epub 2018 Dec 12.
a Noble Research Institute, LLC ., Ardmore , OK , USA.
Sheath blight caused by the soil borne fungus Rhizoctonia solani AG1-IA is one of the major diseases of rice in the world. Genetic resistance in rice against this disease has not been very successful. Brachypodium distachyon is considered as a model species for several cereal crops and it has been studied in the past to identify novel sources of disease resistance against cereal crop diseases. Read More
FASEB J 2018 Dec 12:fj201801498R. Epub 2018 Dec 12.
Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Peroxisomes are essential organelles for the specialized oxidation of a wide variety of fatty acids, but they are also able to degrade fatty acids that are typically handled by mitochondria. Using a combination of pharmacological inhibition and clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 genome editing technology to simultaneously manipulate peroxisomal and mitochondrial fatty acid β-oxidation (FAO) in HEK-293 cells, we identified essential players in the metabolic crosstalk between these organelles. Depletion of carnitine palmitoyltransferase (CPT)2 activity through pharmacological inhibition or knockout (KO) uncovered a significant residual peroxisomal oxidation of lauric and palmitic acid, leading to the production of peroxisomal acylcarnitine intermediates. Read More
JAMA Surg 2018 Dec 12. Epub 2018 Dec 12.
Department of Medicine, Stanford University, Stanford, California.
JAMA Surg 2018 Dec 12. Epub 2018 Dec 12.
OasisMD Lifestyle Healthcare, San Diego, California.
JAMA Psychiatry 2018 Dec 12. Epub 2018 Dec 12.
Department of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.
Importance: Biologic systems involved in the regulation of motor activity are intricately linked with other homeostatic systems such as sleep, feeding behavior, energy, and mood. Mobile monitoring technology (eg, actigraphy and ecological momentary assessment devices) allows the assessment of these multiple systems in real time. However, most clinical studies of mental disorders that use mobile devices have not focused on the dynamic associations between these systems. Read More
Canine Genet Epidemiol 2018 29;5(Suppl 1). Epub 2018 Aug 29.
2The Royal (Dick) School for Veterinary Studies and Roslin Institute, University of Edinburgh, Easter Bush, Midlothian, UK.
J Water Health 2018 Dec;16(6):991-1006
Department of Aquatic Production, Abel Salazar Institute for the Biomedical Sciences (ICBAS), University of Porto, 4050-313 Porto, Portugal E-mail: LAQV, Requimte, Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, 4169-007 Porto, Portugal.
The Ave River in northern Portugal has a history of riverbanks and water quality degradation. The river water quality was assessed by physicochemical, biological (macroinvertebrates) and microbiological (Enterococcus spp. and Escherichia coli) parameters in six locations (A-F, point A being the nearest to the source) throughout its course during a year. Read More
Aging (Albany NY) 2018 Dec 12. Epub 2018 Dec 12.
Department of Neurology, National Neuroscience Institute, Singapore 308433, Singapore.
Individuals with mild cognitive impairment (MCI) exhibit varying serial position effect (SPE) performances. The relationship between SPE performance in word list recall and clinical, genetic, and neuroimaging features of MCI requires elucidation. 119 MCI and 68 cognitively normal (CN) participants underwent cognitive assessment, apolipoprotein E (ApoE) genotyping, and volumetric MRI brain scans processed via voxel-based morphometry. Read More
J Med Microbiol 2018 Dec 12. Epub 2018 Dec 12.
4Department of Medicine, Campus Biomedico University, Via Alvaro del Portillo 200, 00128 Rome, Italy.
Purpose: Staphylococcus aureus isolates, collected from various clinical samples, were analysed to evaluate the contribution of the genetic background of both erythromycin-resistant (ERSA) and -susceptible (ESSA) S. aureus strains to biofilm formation.
Methods: A total of 66 ESSA and 43 ERSA clinical isolates were studied for adhesiveness and biofilm formation under different atmospheres. Read More
J Med Microbiol 2018 Dec 12. Epub 2018 Dec 12.
3Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, USA.
Purpose: This study was aimed at investigating the occurrence and genetic mechanisms of resistance to ciprofloxacin, tetracycline and erythromycin in clinical isolates of Campylobacter jejuni recovered from human cases of acute gastroenteritis in Turkey.
Methodology: MIC values of each antibiotic were determined with the epsilometer test (E-test). Resistance genes/mutations were first screened by PCR and analysed by subsequent DNA sequencing. Read More
J Med Microbiol 2018 Dec 12. Epub 2018 Dec 12.
2Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK.
Purpose: Mycobacteria are common causative agents of bacterial infections in many species of freshwater and marine fish. Identification of mycobacteria to the species level based on phenotypic tests is inappropriate and time consuming. Molecular methods such as partial or entire gene sequence determination in mycobacteria have been employed to resolve these problems. Read More
Physiol Rev 2019 Jan;99(1):853-892
Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego , La Jolla, California ; and Washington University School of Medicine, St. Louis, Missouri.
The central functions fulfilled by mitochondria as both energy generators essential for tissue homeostasis and gateways to programmed apoptotic and necrotic cell death mandate tight control over the quality and quantity of these ubiquitous endosymbiotic organelles. Mitophagy, the targeted engulfment and destruction of mitochondria by the cellular autophagy apparatus, has conventionally been considered as the mechanism primarily responsible for mitochondrial quality control. However, our understanding of how, why, and under what specific conditions mitophagy is activated has grown tremendously over the past decade. Read More