1,387,956 results match your criteria Genes Brain and Behavior [Journal]


Genome-wide association study of degenerative mitral valve disease in Maltese dogs.

J Vet Sci 2018 Dec 4. Epub 2018 Dec 4.

Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Seoul, 05030, Korea.

Genome-wide association study (GWAS) is a powerful tool for identifying the genetic causes of various diseases. This study conducted to identify genomic variation in Maltese dog genomes associated with degenerative mitral valve disease (DMVD) development and evaluate the association of each biological condition with DMVD in Maltese dogs. DNA was extracted from blood samples obtained from 48 Maltese dogs (32 with DMVD and 16 controls). Read More

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December 2018
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MAGE genes in the kidney: identification of MAGED2 as upregulated during kidney injury and in stressed tubular cells.

Nephrol Dial Transplant 2018 Dec 11. Epub 2018 Dec 11.

Nephrology, IIS-Fundacion Jimenez Diaz-Universidad Autonoma de Madrid and Fundacion Renal Iñigo Alvarez de Toledo-IRSIN, Madrid, Spain.

Background: Mutations in Melanoma Antigen-encoding Gene D2 (MAGED2) promote tubular dysfunction, suggesting that MAGE proteins may play a role in kidney pathophysiology. We have characterized the expression and regulation of MAGE genes in normal kidneys and during kidney disease.

Methods: The expression of MAGE genes and their encoded proteins was explored by systems biology multi-omics (kidney transcriptomics and proteomics) in healthy adult murine kidneys and following induction of experimental acute kidney injury (AKI) by a folic acid overdose. Read More

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December 2018

The Gly385(388)Arg Polymorphism of the FGFR4 Receptor regulates Hepatic Lipogenesis under healthy Diet.

J Clin Endocrinol Metab 2018 Dec 12. Epub 2018 Dec 12.

Department of Internal Medicine, Division of Endocrinology, Diabetology, Vascular Disease, Nephrology and Clinical Chemistry, University of Tübingen, Germany.

Context: The effect of a lifestyle intervention to reduce liver fat content in nonalcoholic fatty liver disease in humans is influenced by genetics. We hypothesized that the functionally active amino acid exchange in humans Gly388Arg (mouse homologue: Gly385Arg) in the fibroblast growth factor receptor 4 (FGFR4), which regulates bile acid, lipid and glucose metabolism, may determine the dynamics of hepatic lipid accumulation and insulin sensitivity in humans. Mechanisms of this substitution were studied in mice under normal chow and high-fat diet. Read More

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December 2018
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Expression of GHR and downstream signaling genes in human adipose tissue - relation to obesity and weight-change.

J Clin Endocrinol Metab 2018 Dec 12. Epub 2018 Dec 12.

Department of Molecular and Clinical Medicine, Institute of Medicine at the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Context: Growth hormone (GH) is a strong regulator of metabolism. In obesity, both GH secretion and adipose tissue GHR gene expression are decreased. More detailed information on the regulation of GHR, STAT3/5 and downstream-regulated genes in human adipose tissue during diet-induced weight loss and weight gain is lacking. Read More

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December 2018

Plasmid-based gap-repair recombineered transgenes reveal a central role for introns in mutually exclusive alternative splicing in Down Syndrome Cell Adhesion Molecule exon 4.

Nucleic Acids Res 2018 Dec 12. Epub 2018 Dec 12.

School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.

Alternative splicing is a key feature of human genes, yet studying its regulation is often complicated by large introns. The Down Syndrome Cell Adhesion Molecule (Dscam) gene from Drosophila is one of the most complex genes generating vast molecular diversity by mutually exclusive alternative splicing. To resolve how alternative splicing in Dscam is regulated, we first developed plasmid-based UAS reporter genes for the Dscam variable exon 4 cluster and show that its alternative splicing is recapitulated by GAL4-mediated expression in neurons. Read More

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December 2018

The role of glutamine metabolism for host defense against Mycobacterium tuberculosis infection.

J Infect Dis 2018 Dec 12. Epub 2018 Dec 12.

Radboud university medical center, Department of Internal Medicine and Radboud Center of Infectious Diseases, Nijmegen, the Netherlands.

Background: Rewiring cellular metabolism is important for activation of immune cells during host defense against Mycobacterium tuberculosis. Glutamine has been implicated as an immunomodulatory nutrient, but its role in response to M. tuberculosis is unknown. Read More

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December 2018
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Galapagos Islands Endemic Vertebrates: A Population Genetics Perspective.

Authors:
Philip W Hedrick

J Hered 2018 Dec 12. Epub 2018 Dec 12.

School of Life Sciences, Arizona State University, Tempe, AZ.

The organisms of the Galapagos Islands played a central role in the development of the theory of evolution by Charles Darwin. Examination of the population genetics factors of many of these organisms with modern molecular methods have expanded our understanding of their evolution. Here I provide a perspective on how selection, gene flow, genetic drift, mutation, and inbreeding have contributed to the evolution of six of the iconic Galapagos species; flightless cormorant, pink iguana, marine iguana, Galapagos hawk, giant tortoises, and Darwin's finches. Read More

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December 2018

KDM5D-mediated H3K4 demethylation is required for sexually dimorphic gene expression in mouse embryonic fibroblasts.

J Biochem 2018 Dec 12. Epub 2018 Dec 12.

Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, Japan.

Males and females share the same genetic code, but gene expression profile often displays differences between two sexes. Mouse embryonic fibroblasts (MEFs) have been used to experiment as a useful tool to test gene function. They have also been characterized by gender-based differences in expressed genes such as Y-linked Sry or X-linked Hprt. Read More

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December 2018

DNA demethylation facilitates the specific transcription of the mouse X-linked Tsga8 gene in round spermatids.

Biol Reprod 2018 Dec 12. Epub 2018 Dec 12.

Department of Medical Genetics and Division of Human Morbid Genomics, State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan Province, China.

Some X-linked genes necessary for spermiogenesis are specifically activated in the post-meiotic germ cells. However, the regulatory mechanism about this activation is not clearly understood. Here, we examined the potential mechanism controlling the transcriptional activation of the mouse testis specific gene A8 (Tsga8) gene in round spermatids. Read More

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December 2018

Gene expression models based on transcription factor binding events confer insight into functional cis-regulatory variants.

Bioinformatics 2018 Dec 12. Epub 2018 Dec 12.

Centre for Molecular Medicine and Therapeutics, Department of Medical Genetics, BC Children's Hospital Research Institute, University of British Columbia, Vancouver, Canada.

Motivation: Deciphering the functional roles of cis-regulatory variants is a critical challenge in genome analysis and interpretation. It has been hypothesized that altered transcription factor (TF) binding events are a central mechanism by which cis-regulatory variants impact gene expression levels. However, we lack a computational framework to understand and quantify such mechanistic contributions. Read More

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December 2018

miR172 Regulates both Vegetative and Reproductive Development in the Perennial Woody Plant Jatropha curcas.

Plant Cell Physiol 2018 Dec;59(12):2549-2563

CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Mengla, Yunnan, China.

Jatropha curcas is a promising feedstock for biofuel production because its oil is highly suitable for processing bio-jet fuels and biodiesel. However, Jatropha exhibits a long juvenile stage in subtropical areas. miR172, a conserved small non-protein-coding RNA molecule with 21 nucleotides, regulates a wide range of developmental processes. Read More

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December 2018

Agnostic Pathway/Gene Set Analysis of Genome-Wide Association Data Identifies Associations for Pancreatic Cancer.

Authors:
Naomi Walsh Han Zhang Paula L Hyland Qi Yang Evelina Mocci Mingfeng Zhang Erica J Childs Irene Collins Zhaoming Wang Alan A Arslan Laura Beane-Freeman Paige M Bracci Paul Brennan Federico Canzian Eric J Duell Steven Gallinger Graham G Giles Michael Goggins Gary E Goodman Phyllis J Goodman Rayjean J Hung Charles Kooperberg Robert C Kurtz Núria Malats Loic LeMarchand Rachel E Neale Sara H Olson Ghislaine Scelo Xiao O Shu Stephen K Van Den Eeden Kala Visvanathan Emily White Wei Zheng Demetrius Albanes Gabriella Andreotti Ana Babic William R Bamlet Sonja I Berndt Ayelet Borgida Marie-Christine Boutron-Ruault Lauren Brais Paul Brennan Bas Bueno-de-Mesquita Julie Buring Kari G Chaffee Stephen Chanock Sean Cleary Michelle Cotterchio Lenka Foretova Charles Fuchs J Michael M Gaziano Edward Giovannucci Michael Goggins Thilo Hackert Christopher Haiman Patricia Hartge Manal Hasan Kathy J Helzlsouer Joseph Herman Ivana Holcatova Elizabeth A Holly Robert Hoover Rayjean J Hung Vladimir Janout Eric A Klein Robert C Kurtz Daniel Laheru I-Min Lee Lingeng Lu Núria Malats Satu Mannisto Roger L Milne Ann L Oberg Irene Orlow Alpa V Patel Ulrike Peters Miquel Porta Francisco X Real Nathaniel Rothman Howard D Sesso Gianluca Severi Debra Silverman Oliver Strobel Malin Sund Mark D Thornquist Geoffrey S Tobias Jean Wactawski-Wende Nick Wareham Elisabete Weiderpass Nicolas Wentzensen William Wheeler Herbert Yu Anne Zeleniuch-Jacquotte Peter Kraft Donghui Li Eric J Jacobs Gloria M Petersen Brian M Wolpin Harvey A Risch Laufey T Amundadottir Kai Yu Alison P Klein Rachael Z Stolzenberg-Solomon

J Natl Cancer Inst 2018 Dec 12. Epub 2018 Dec 12.

Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD.

Background: Genome-wide association studies (GWAS) identify associations of individual single-nucleotide polymorphisms (SNPs) with cancer risk but usually only explain a fraction of the inherited variability. Pathway analysis of genetic variants is a powerful tool to identify networks of susceptibility genes.

Methods: We conducted a large agnostic pathway-based meta-analysis of GWAS data using the summary-based adaptive rank truncated product method to identify gene sets and pathways associated with pancreatic ductal adenocarcinoma (PDAC) in 9040 cases and 12 496 controls. Read More

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December 2018

CD33 in Alzheimer's Disease - Biology, Pathogenesis, and Therapeutics: A Mini-Review.

Authors:
Lingzhi Zhao

Gerontology 2018 Dec 12:1-9. Epub 2018 Dec 12.

Alzheimer's disease (AD) affects nearly 50 million people worldwide, and currently no disease-modifying treatment is available. With continuous failure of anti-amyloid-beta- or tau-based therapies, identification of new targets has become an urgent necessity for AD prevention and therapy. Recently, conventional genetic approaches and computational strategies have converged on immune-inflammatory pathways as key events in the pathogenesis of AD. Read More

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December 2018
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Comparative Study of MSCA-1 and CD146 Isolated Periosteal Cell Subpopulations.

Cell Physiol Biochem 2018 12;51(3):1193-1206. Epub 2018 Dec 12.

Department of Oral and Maxillofacial Surgery of the University Hospital of Tübingen, Tübingen,

Background/aims: Periosteal tissue is a valuable source of multipotent stem cells for bone tissue engineering. To characterize these cells in detail, we generated an immortalized human cranial periosteal cell line and observed an increased MSCA-1 and CD146 expression, as well as an earlier and stronger mineralization compared to the parental cells. Further, we detected a higher osteogenic potential of MSCA-1high compared to MSCA-1low cranial periosteal cell (CPC) fractions. Read More

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December 2018

Axon-Seq Decodes the Motor Axon Transcriptome and Its Modulation in Response to ALS.

Stem Cell Reports 2018 Dec;11(6):1565-1578

Department of Neuroscience, Karolinska Institutet, Stockholm 171 77, Sweden. Electronic address:

Spinal motor axons traverse large distances to innervate target muscles, thus requiring local control of cellular events for proper functioning. To interrogate axon-specific processes we developed Axon-seq, a refined method incorporating microfluidics, RNA sequencing (RNA-seq), and bioinformatic quality control. We show that the axonal transcriptome is distinct from that of somas and contains fewer genes. Read More

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December 2018

The antiviral mechanism of viperin and its splice variant in spring viremia of carp virus infected fathead minnow cells.

Fish Shellfish Immunol 2018 Dec 9. Epub 2018 Dec 9.

College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, 430070, China; Hubei Engineering Technology Research Center for Aquatic Animal Diseases Control and Prevention, Wuhan, 430070, China. Electronic address:

Viperin is known to play an important role in innate immune and its antiviral mechanisms are well demonstrated in mammals. Fish Viperin mediates antiviral activity against several viruses. However, little has been done to the underlying mechanism. Read More

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December 2018

Importin α5 Regulates Anxiety through MeCP2 and Sphingosine Kinase 1.

Cell Rep 2018 Dec;25(11):3169-3179.e7

Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot 76100, Israel. Electronic address:

Importins mediate transport from synapse to soma and from cytoplasm to nucleus, suggesting that perturbation of importin-dependent pathways should have significant neuronal consequences. A behavioral screen on five importin α knockout lines revealed that reduced expression of importin α5 (KPNA1) in hippocampal neurons specifically decreases anxiety in mice. Re-expression of importin α5 in ventral hippocampus of knockout animals increased anxiety behaviors to wild-type levels. Read More

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December 2018

V2-Directed Vaccine-like Antibodies from HIV-1 Infection Identify an Additional K169-Binding Light Chain Motif with Broad ADCC Activity.

Cell Rep 2018 Dec;25(11):3123-3135.e6

National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg 2131, South Africa; Faculty of Health Sciences, University of the Witwatersrand, Johannesburg 2000, South Africa; Centre for the AIDS Programme of Research in South Africa (CAPRISA), University of KwaZulu Natal, Durban 4041, South Africa. Electronic address:

Antibodies that bind residue K169 in the V2 region of the HIV-1 envelope correlated with reduced risk of infection in the RV144 vaccine trial but were restricted to two ED-motif-encoding light chain genes. Here, we identify an HIV-infected donor with high-titer V2 peptide-binding antibodies and isolate two antibody lineages (CAP228-16H/19F and CAP228-3D) that mediate potent antibody-dependent cell-mediated cytotoxicity (ADCC). Both lineages use the IGHV5-51 heavy chain germline gene, similar to the RV144 antibody CH58, but one lineage (CAP228-16H/19F) uses a light chain without the ED motif. Read More

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December 2018

TMED2 Potentiates Cellular IFN Responses to DNA Viruses by Reinforcing MITA Dimerization and Facilitating Its Trafficking.

Cell Rep 2018 Dec;25(11):3086-3098.e3

State Key Laboratory of Virology, Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan 430072, China. Electronic address:

Mediator of IRF3 activation (MITA), also known as stimulator of interferon genes (STING), plays a vital role in the innate immune responses to cytosolic dsDNA. The trafficking of MITA from the ER to perinuclear vesicles is necessary for its activation of the downstream molecules, which lead to the production of interferons and pro-inflammatory cytokines. However, the exact mechanism of MITA activation remains elusive. Read More

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December 2018
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KLF4, A Gene Regulating Prostate Stem Cell Homeostasis, Is a Barrier to Malignant Progression and Predictor of Good Prognosis in Prostate Cancer.

Cell Rep 2018 Dec;25(11):3006-3020.e7

Department of Cell Biology, NYU School of Medicine, New York, NY 10016, USA; Department of Urology, NYU School of Medicine, New York, NY 10016, USA. Electronic address:

There is a considerable need to identify those individuals with prostate cancer who have indolent disease. We propose that genes that control adult stem cell homeostasis in organs with slow turnover, such as the prostate, control cancer fate. One such gene, KLF4, overexpressed in murine prostate stem cells, regulates their homeostasis, blocks malignant transformation, and controls the self-renewal of tumor-initiating cells. Read More

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December 2018

Gene network analysis of poplar root transcriptome in response to drought stress identifies a PtaJAZ3PtaRAP2.6-centered hierarchical network.

PLoS One 2018 12;13(12):e0208560. Epub 2018 Dec 12.

Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI, United States of America.

Using time-series transcriptomic data from poplar roots undergoing polyethylene glycol (PEG)-induced drought stress, we built a genetic network model of the involved putative molecular responses. We found that the network resembled a hierarchical structure. The highest hierarchical level in this structure is occupied by 9 genes, which we called superhubs because they were primarily connected to 18 hub genes, which are then connected to 2,934 terminal genes. Read More

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December 2018

Differential gene expression between the vigorous and dwarf litchi cultivars based on RNA-Seq transcriptome analysis.

PLoS One 2018 12;13(12):e0208771. Epub 2018 Dec 12.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China) in Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou, China.

Litchi (Litchi chinesis Sonn.) is the most economically significant member of Sapindaceae family, especially in sub-tropical regions. However, its tall tree body often brings many inconveniences to production management. Read More

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December 2018

Antimicrobial resistance, plasmid, virulence, multilocus sequence typing and pulsed-field gel electrophoresis profiles of Salmonella enterica serovar Typhimurium clinical and environmental isolates from India.

PLoS One 2018 12;13(12):e0207954. Epub 2018 Dec 12.

Division of Bacteriology, Indian Council of Medical Research-National Institute of Cholera and Enteric Diseases, Kolkata, West Bengal, India.

Salmonella enterica serovar Typhimurium (S. Typhimurium) is a common serovar associated with non-typhoidal salmonellosis globally. However, there is insufficient data on molecular characterization of S. Read More

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December 2018

A transient DMSO treatment increases the differentiation potential of human pluripotent stem cells through the Rb family.

PLoS One 2018 12;13(12):e0208110. Epub 2018 Dec 12.

Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, Stanford, California, United States of America.

The propensity for differentiation varies substantially across human pluripotent stem cell (hPSC) lines, greatly restricting the use of hPSCs for cell replacement therapy or disease modeling. Here, we investigate the underlying mechanisms and demonstrate that activation of the retinoblastoma (Rb) pathway in a transient manner is important for differentiation. In prior work, we demonstrated that pre-treating hPSCs with dimethylsulfoxide (DMSO) before directed differentiation enhanced differentiation potential across all three germ layers. Read More

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December 2018
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Genome-wide identification and characterization of gene family for RWP-RK transcription factors in wheat (Triticum aestivum L.).

PLoS One 2018 12;13(12):e0208409. Epub 2018 Dec 12.

Department of Genetics and Plant Breeding, CCS University, Meerut, India.

RWP-RKs represent a small family of transcription factors (TFs) that are unique to plants and function particularly under conditions of nitrogen starvation. These RWP-RKs have been classified in two sub-families, NLPs (NIN-like proteins) and RKDs (RWP-RK domain proteins). NLPs regulate tissue-specific expression of genes involved in nitrogen use efficiency (NUE) and RKDs regulate expression of genes involved in gametogenesis/embryogenesis. Read More

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December 2018
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Gamma secretase dependent release of the CD44 cytoplasmic tail upregulates IFI16 in cd44-/- tumor cells, MEFs and macrophages.

PLoS One 2018 12;13(12):e0207358. Epub 2018 Dec 12.

Leibniz Institute on Aging, Fritz Lipmann Institute (FLI), Jena, Germany.

The adhesion molecule and co-receptor of receptor tyrosine kinases, CD44, is expressed in all cells of the immune system, but also in numerous non-immune cells. CD44 plays roles in the cellular response to different pathogens. The molecular actions of CD44 during these processes are by and large still unknown. Read More

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December 2018

Coordinated regulation of transcription by CcpA and the Staphylococcus aureus two-component system HptRS.

PLoS One 2018 12;13(12):e0207161. Epub 2018 Dec 12.

School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America.

The success of Staphylococcus aureus as a pathogen is due in part to its ability to adapt to changing environmental conditions using signal transduction pathways, such as metabolite- responsive regulators and two-component systems. S. aureus has a two-component system encoded by the gene pair sav0224 (hptS) and sav0223 (hptR) that regulate the hexose phosphate transport (uhpT) system in response to extracellular glucose-6-phosphate. Read More

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December 2018

Multiplexed CRISPR/Cas9-mediated knockout of 19 Fanconi anemia pathway genes in zebrafish revealed their roles in growth, sexual development and fertility.

PLoS Genet 2018 Dec 12;14(12):e1007821. Epub 2018 Dec 12.

Cancer Genomics Unit, Cancer Genetics and Comparative Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, United States of America.

Fanconi Anemia (FA) is a genomic instability syndrome resulting in aplastic anemia, developmental abnormalities, and predisposition to hematological and other solid organ malignancies. Mutations in genes that encode proteins of the FA pathway fail to orchestrate the repair of DNA damage caused by DNA interstrand crosslinks. Zebrafish harbor homologs for nearly all known FA genes. Read More

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December 2018
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Boundaries mediate long-distance interactions between enhancers and promoters in the Drosophila Bithorax complex.

PLoS Genet 2018 Dec 12;14(12):e1007702. Epub 2018 Dec 12.

Department of the Control of Genetic Processes, Institute of Gene Biology Russian Academy of Sciences, Moscow, Russia.

Drosophila bithorax complex (BX-C) is one of the best model systems for studying the role of boundaries (insulators) in gene regulation. Expression of three homeotic genes, Ubx, abd-A, and Abd-B, is orchestrated by nine parasegment-specific regulatory domains. These domains are flanked by boundary elements, which function to block crosstalk between adjacent domains, ensuring that they can act autonomously. Read More

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December 2018

Decomposing the subclonal structure of tumors with two-way mixture models on copy number aberrations.

PLoS One 2018 12;13(12):e0206579. Epub 2018 Dec 12.

Institute of Statistics, National Tsing Hua University, Hsinchu, Taiwan, R.O.C.

Multistage tumorigenesis is a dynamic process characterized by the accumulation of mutations. Thus, a tumor mass is composed of genetically divergent cell subclones. With the advancement of next-generation sequencing (NGS), mathematical models have been recently developed to decompose tumor subclonal architecture from a collective genome sequencing data. Read More

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December 2018

Heterochromatin delays CRISPR-Cas9 mutagenesis but does not influence the outcome of mutagenic DNA repair.

PLoS Biol 2018 Dec 12;16(12):e2005595. Epub 2018 Dec 12.

MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, United Kingdom.

Genome editing occurs in the context of chromatin, which is heterogeneous in structure and function across the genome. Chromatin heterogeneity is thought to affect genome editing efficiency, but this has been challenging to quantify due to the presence of confounding variables. Here, we develop a method that exploits the allele-specific chromatin status of imprinted genes in order to address this problem in cycling mouse embryonic stem cells (mESCs). Read More

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December 2018
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Secreted Factors From Intervertebral Disc Cells and Infiltrating Macrophages Promote Degenerated Intervertebral Disc Catabolism.

Spine (Phila Pa 1976) 2018 Dec 11. Epub 2018 Dec 11.

Department of Spine Surgery, Beijing Jishuitan Hospital, Peking University, Beijing, China.

Study Design: Rat nucleus pulposus (NP) cells or annulus fibrosus (AF) cells were stimulated with conditioned media of RAW 264.7 macrophages and vice versa under healthy culture conditions and in the presence of pro-inflammatory mediators. The gene expression of pro-inflammatory mediators, ECM-modifying enzymes, and chemokines, which play important roles in intervertebral disc degeneration (IDD), was determined. Read More

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December 2018

Decreased Fecal Bacterial Diversity and Altered Microbiome in Children Colonized With Clostridium difficile.

J Pediatr Gastroenterol Nutr 2018 Dec 11. Epub 2018 Dec 11.

Department of Medicine, Johns Hopkins University School of Medicine.

Objectives: The gut microbiome is believed to play a role in the susceptibility to and treatment of Clostridium difficile infections (CDIs). It is, however, unknown whether the gut microbiome is also affected by asymptomatic C difficile colonization. Our study aimed to evaluate the fecal microbiome of children based on C difficile colonization, and CDI risk factors, including antibiotic use and comorbid inflammatory bowel disease (IBD). Read More

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December 2018

Differential Gene Expression in Erlotinib-treated Fibroblasts.

Nurs Res 2018 Dec 12. Epub 2018 Dec 12.

Assistant Professor, University of South Carolina, College of Nursing, Columbia, SC Bioinformatics Analyst I, University of Maryland, Baltimore, School of Medicine, Institute for Genome Sciences, Baltimore, MD Professor, Chair, Department of Hematology/Oncology, Deputy Director of the Cancer Center for Clinical Research, G. Morris Dorrance Chair in Medical Oncology, Fox Chase Cancer Center, Philadelphia, PA Bioinformatics Analyst II, University of Maryland, School of Medicine, Institute for Genome Sciences, Baltimore, MD Laboratory Research Technician, University of Maryland, Baltimore, School of Nursing, Baltimore, MD Professor, Chair, Department of Pain and Translational Symptom Science, University of Maryland, Baltimore, School of Nursing, Baltimore, MD.

Background: Therapies targeting the epidermal growth factor receptor (EGFR) result in a painful rash, the most common and debilitating toxicity among patients with non-small cell lung cancer (NSCLC) who take EGFR tyrosine kinase inhibitor (TKI) therapy; however, predicting the development and the severity of the rash is difficult.

Objective: To examine how erlotinib-an EGFR TKI that NSCLC patients take to stop or slow tumor growth-altered the transcriptome of dermal fibroblasts.

Methods: Dermal fibroblasts (ATCC® PCS-201-012™) were seeded in cell culture flasks, grown under standard conditions, and transferred to cell culture dishes. Read More

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December 2018

LongShengZhi capsule reduces established atherosclerotic lesions in apoE deficient mice by ameliorating hepatic lipid metabolism and inhibiting inflammation.

J Cardiovasc Pharmacol 2018 Dec 10. Epub 2018 Dec 10.

College of Biomedical Engineering, Hefei University of Technology, Hefei, China.

Disorders of lipid metabolism and inflammation play an important role in atherosclerosis. LongShengZhi capsule (LSZ), a Chinese herbal medicine, has been used for treatment of patients with vascular diseases for many years. Herein, we determined the effect of LSZ on the progression of established atherosclerotic lesions in apoE deficient (apoE) mice. Read More

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December 2018

Gene-function studies in SLE.

Curr Opin Rheumatol 2018 Dec 11. Epub 2018 Dec 11.

Department of Immunology and Rheumatology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán.

Purpose Of Review: The aim of this review is to discuss recent developments in our understanding of how systemic lupus erythematosus (SLE)-associated genes contribute to autoimmunity.

Recent Findings: Gene-function studies have revealed mechanisms through which SLE-associated alleles of IFIH1, TNFAIP3, IRF5, and PRDM1 likely contribute to the development of autoimmunity. Novel research has identified Mac-1 (encoded by ITGAM), CaMK4, and iRhom2 as plausible therapeutic targets in lupus nephritis. Read More

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December 2018
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Identification of molecular signatures of cystic fibrosis disease status using plasma-based functional genomics

Physiol Genomics 2018 12 12. Epub 2018 Dec 12.

Department of Pediatrics, Medical College of Wisconsin, United States.

Although cystic fibrosis (CF) is attributed to dysfunction of a single gene, the relationships between the abnormal gene product and development of inflammation and progression of lung disease are not fully understood, limiting our ability to predict an individual patient's clinical course and treatment response. To better understand CF progression, we characterized the molecular signatures of CF disease status using plasma-based functional genomics. Peripheral blood mononuclear cells (PBMCs) from healthy donors were cultured with plasma samples from CF patients (n=103) and unrelated, healthy controls (n=31). Read More

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December 2018
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The Dual Effect of the Brassinosteroid Pathway on Rice Black Streaked Dwarf Virus Infection by Modulating the Peroxidase-Mediated Oxidative Burst and Plant Defense.

Mol Plant Microbe Interact 2018 Dec 12. Epub 2018 Dec 12.

Fenghua Road 818,Ningbo, China , 315211 ;

The phytohormone brassinosteroids (BR) not only played key roles in regulating plant growth development but also involved in modulating plant defense system upon pathogens attack. Our previous study found that BR pathway was susceptible to Rice Blacked-Streaked Dwarf Virus (RBSDV), a devastating pathogen in rice. However, the detailed mechanism of BR-mediated susceptibility remains unclear. Read More

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December 2018

Less is more: towards a genome-reduced Bacillus cell factory for 'difficult proteins'.

ACS Synth Biol 2018 Dec 12. Epub 2018 Dec 12.

The availability of complete genome sequences and the definition of essential gene sets were fundamental in the start of the genome engineering era. In a recent study, redundant and unnecessary genes were systematically deleted from the Gram-positive bacterium Bacillus subtilis, an industrial production host of high-value secreted proteins. This culminated in strain PG10, which lacks about 36% of the genome, thus representing the most minimal Bacillus chassis currently available. Read More

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December 2018

Antibiotic resistance of Campylobacter jejuni isolates recovered from humans with diarrhoea in Turkey.

J Med Microbiol 2018 Dec 12. Epub 2018 Dec 12.

3​Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, USA.

Purpose: This study was aimed at investigating the occurrence and genetic mechanisms of resistance to ciprofloxacin, tetracycline and erythromycin in clinical isolates of Campylobacter jejuni recovered from human cases of acute gastroenteritis in Turkey.

Methodology: MIC values of each antibiotic were determined with the epsilometer test (E-test). Resistance genes/mutations were first screened by PCR and analysed by subsequent DNA sequencing. Read More

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December 2018

Vallitalea okinawensis sp. nov., isolated from Okinawa Trough sediment and emended description of the genus Vallitalea.

Int J Syst Evol Microbiol 2018 Dec 12. Epub 2018 Dec 12.

4​RCEECAS Joint-Lab of Microbial Technology for Environmental Science, Beijing, PR China.

A polyphasic study was conducted to characterize an obligately anaerobic bacterial strain, S15, that was isolated from Okinawa Trough sediment. Strain S15 was Gram-stain-negative, non-motile and rod-shaped. Spores were not observed. Read More

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December 2018

Natural products as new antimitotic compounds for anticancer drug development.

Clinics (Sao Paulo) 2018 Dec 10;73(suppl 1):e813s. Epub 2018 Dec 10.

Laboratorio de Oncologia Experimental, Nucleo de Pesquisa e Desenvolvimento de Medicamentos (NPDM), Universidade Federal do Ceara, Fortaleza, CE, BR.

Cell cycle control genes are frequently mutated in cancer cells, which usually display higher rates of proliferation than normal cells. Dysregulated mitosis leads to genomic instability, which contributes to tumor progression and aggressiveness. Many drugs that disrupt mitosis have been studied because they induce cell cycle arrest and tumor cell death. Read More

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December 2018

Characterisation of plasmid-mediated rmtB-1 in Enterobacteriaceae clinical isolates from São Paulo, Brazil.

Mem Inst Oswaldo Cruz 2018 Dec 10;113(12):e180392. Epub 2018 Dec 10.

Universidade Federal de São Paulo, Escola Paulista de Medicina, Departamento de Medicina Interna, Divisão de Doenças Infecciosas, Laboratório Alerta, São Paulo, SP, Brasil.

OBJECTIVES The emergence of 16S rRNA methyltranferases (16 RMTAses) has jeopardised the clinical use of aminoglycosides. RmtB is one of the most frequently reported in Gram-negatives worldwide. In this study, we aimed to estimate the frequency of 16S RMTAses encoding genes in Enterobacteriaceae isolated in a three-month period from a tertiary Brazilian hospital. Read More

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December 2018

Polymorphisms in the TGFB1 and IL2RA genes are associated with clinical forms of leprosy in Brazilian population.

Mem Inst Oswaldo Cruz 2018 Dec 10;113(12):e180274. Epub 2018 Dec 10.

Instituto Lauro de Souza Lima, Bauru, SP, Brasil.

BACKGROUND Leprosy is a chronic infectious disease caused by Mycobacterium leprae, and compromises the skin and peripheral nerves. This disease has been classified as multibacillary (MB) or paucibacillary (PB) depending on the host immune response. Genetic epidemiology studies in leprosy have shown the influence of human genetic components on the disease outcomes. Read More

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December 2018
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Complete and de novo assembly of the Leishmania braziliensis (M2904) genome.

Mem Inst Oswaldo Cruz 2018 Dec 10;114:e180438. Epub 2018 Dec 10.

Universidad Autónoma de Madrid, Centro de Biología Molecular Severo Ochoa, Madrid, Spain.

Leishmania braziliensis is the etiological agent of American mucosal leishmaniasis, one of the most severe clinical forms of leishmaniasis. Here, we report the assembly of the L. braziliensis (M2904) genome into 35 continuous chromosomes. Read More

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December 2018

Adipose tissue is less responsive to food restriction anti-inflammatory effects than liver, muscle, and brain in mice.

Braz J Med Biol Res 2018 Dec 10;52(1):e8150. Epub 2018 Dec 10.

Departamento de Farmacologia e Terapêutica, Universidade Estadual de Maringá, Maringá, PR, Brasil.

High caloric intake promotes chronic inflammation, insulin resistance, and chronic diseases such as type-2 diabetes, which may be prevented by food restriction (FR). The effect of FR on expression of pro-inflammatory and anti-inflammatory genes in adipose tissue, liver, muscle, and brain was compared. Male Swiss mice were submitted to FR (FR group) or had free access to food (control group) during 56 days. Read More

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December 2018

Correction for Rosa et al., "Draft Genome Sequence of the (syn., ) Type Strain CBS 9494".

Microbiol Resour Announc 2018 Sep 13;7(10). Epub 2018 Sep 13.

ISVV, Oenology Research Unit EA 4577, USC 1366 INRA, Bordeaux INP, University of Bordeaux, Bordeaux, Villenave d'Ornon, France.

[This corrects the article DOI: 10.1128/MRA.00872-18. Read More

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September 2018

Tracking significant modules and key genes for esophageal squamous cell carcinoma based on differential modules.

J Cancer Res Ther 2018 Dec;14(Supplement):S1135-S1140

Department of General Surgery, Peking University International Hospital, Beijing 100026, China.

Background: The exact molecular mechanism of esophageal squamous cell carcinoma (ESCC) is still unknown, and the prognosis of ESCC has not been significantly improved.

Objective: To understand the molecular mechanism of ESCC, differential modules (DMs) and key genes were identified through conducting analysis on the differential co-expression network (DCN) based on the gene expression profiles of ESCC and protein-protein interaction (PPI) data.

Materials And Methods: First, gene expression profiles of ESCC and PPI data recruiting and preprocessing were conducted; then, a DCN was constructed based on the gene co-expression and gene differential expression in ESCC; in the following, candidate DMs were mined from DCN through a systemic module searching strategy, and significance analysis was performed on candidate DMs to identify DMs; moreover, significant genes contained in the DMs were analyzed to identify the underlying biomarkers for ESCC. Read More

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December 2018

Radioprotective effect of melatonin on expression of and genes in rat peripheral blood.

J Cancer Res Ther 2018 Dec;14(Supplement):S1070-S1075

Department of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Objective: Ionizing radiation is a critical threat to biomolecules, especially DNA. Various combinatorial compounds have been studied to protect this biomolecule. Melatonin has been reported as a direct and indirect free radical scavenger, but in this study, we explored the effect of melatonin on assisting in DNA repair by expression of Cdkn1a and Rad50; both of these genes are involved in DNA repair signaling, induced by radiation in rat peripheral blood. Read More

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December 2018
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Network strategy to investigate differential pathways in sporadic amyotrophic lateral sclerosis.

J Cancer Res Ther 2018 Dec;14(Supplement):S1057-S1062

Department of Internal Medicine of TCM, Zhonglu Hospital, Shandong University of TCM, Jinan, Shandong Province, China.

Objective: The objective of this paper was to investigate differential pathways in sporadic amyotrophic lateral sclerosis (SALS) based on pathway network analysis.

Materials And Methods: To achieve this goal, first, differentially expressed genes (DEGs) between SALS and normal controls were identified, and a target network was defined as DEGs correlated interactions from the search tool for the retrieval of interacting genes/proteins (STRING). Second, topological centrality analysis was conducted on the target network to identify hub genes and hub network. Read More

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December 2018
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