1,415,422 results match your criteria Genes & Development [Journal]


The widespread increase in inter-individual variability of gene expression in the human brain with age.

Aging (Albany NY) 2019 Apr 19. Epub 2019 Apr 19.

European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, CB10 1SD, UK.

Aging is broadly defined as a time-dependent progressive decline in the functional and physiological integrity of organisms. Previous studies and evolutionary theories of aging suggest that aging is not a programmed process but reflects dynamic stochastic events. In this study, we test whether transcriptional noise shows an increase with age, which would be expected from stochastic theories. Read More

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http://dx.doi.org/10.18632/aging.101912DOI Listing

Effect of plant-based diets with varying ratios of ω6 to ω3 fatty acids on growth performance, tissue composition, fatty acid biosynthesis and lipid-related gene expression in Atlantic salmon (Salmo salar).

Comp Biochem Physiol Part D Genomics Proteomics 2019 Mar 16;30:290-304. Epub 2019 Mar 16.

Department of Ocean Sciences, Memorial University of Newfoundland, St. John's A1C 5S7, NL. Canada. Electronic address:

Little is known about how variation in omega-6 to omega-3 (ω6:ω3) fatty acid (FA) ratios affects lipid metabolism and eicosanoid synthesis in salmon, and the potential underlying molecular mechanisms. The current study examined the impact of five plant-based diets (12-week exposure) with varying ω6:ω3 (0.3-2. Read More

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http://dx.doi.org/10.1016/j.cbd.2019.03.004DOI Listing

Chicken viperin inhibits Newcastle disease virus infection in vitro: A possible interaction with the viral matrix protein.

Cytokine 2019 Apr 16;120:28-40. Epub 2019 Apr 16.

Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India. Electronic address:

Viperin is an interferon-inducible protein that helps in protecting mammals against various virus infections. Viperin is a highly conserved member of the interferon-stimulated genes (ISG) family in many species. Viperin has been shown to play a pivotal role in the innate immunity of chicken; however, its role has not been explored in its antiviral potential. Read More

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http://dx.doi.org/10.1016/j.cyto.2019.04.007DOI Listing

Co-assembled Ca Alginate-Sulfate Nanoparticles for Intracellular Plasmid DNA Delivery.

Mol Ther Nucleic Acids 2019 Mar 28;16:378-390. Epub 2019 Mar 28.

Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel; Regenerative Medicine and Stem Cell (RMSC) Research Center, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel; The Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel. Electronic address:

Successful gene therapy requires the development of suitable carriers for the selective and efficient delivery of genes to specific target cells, with minimal toxicity. In this work, we present a non-viral vector for gene delivery composed of biocompatible materials, CaCl, plasmid DNA and the semi-synthetic anionic biopolymer alginate sulfate (AlgS), which spontaneously co-assembled to form nanoparticles (NPs). The NPs were characterized with a slightly anionic surface charge (Zeta potential [ζ] = -14 mV), an average size of 270 nm, and their suspension was stable for several days with no aggregation. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S21622531193006
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http://dx.doi.org/10.1016/j.omtn.2019.03.006DOI Listing
March 2019
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Comparative study of Cu uptake and early transcriptome responses in the green microalga Chlamydomonas reinhardtii and the macrophyte Elodea nuttallii.

Environ Pollut 2019 Apr 10;250:331-337. Epub 2019 Apr 10.

Department F.-A. Forel for Environmental and Aquatic Sciences, Earth and Environmental Sciences, Faculty of Sciences, University of Geneva, 66, Boulevard Carl-Vogt, CH-1211, Geneva 4, Switzerland. Electronic address:

Microalgae are widely used as representative primary producers in ecotoxicology, while macrophytes are much less studied. Here we compared the bioavailability and cellular toxicity pathways of 2 h-exposure to 10 mol L Cu in the macrophyte Elodea nuttallii and the green microalga Chlamydomonas reinhardtii. Uptake rate was similar but faster in the algae than in the macrophyte, while RNA-Sequencing revealed a similar number of regulated genes. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S02697491183557
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http://dx.doi.org/10.1016/j.envpol.2019.04.032DOI Listing
April 2019
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PCB52 induces hepatotoxicity in male offspring through aggravating loss of clearance capacity and activating the apoptosis: Sex-biased effects on rats.

Chemosphere 2019 Apr 12;227:389-400. Epub 2019 Apr 12.

Department of Forensic Pathology, School of Forensic Medicine, Southern Medical University, No. 1838 North Guangzhou Road, 510515, Guangzhou, China. Electronic address:

Polychlorinated biphenyls (PCBs), a kind of persistent organic pollutant, can induce hepatotoxicity in mammals. However, PCB-induced hepatotoxicity in offspring and the underlying mechanisms have been rarely studied. In the present study, Wistar rats were administered with corn oil or PCB52 (1 mg/kg body weight/day, by gavage) from gestational day 7 to postnatal day 21. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00456535193072
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http://dx.doi.org/10.1016/j.chemosphere.2019.04.077DOI Listing
April 2019
2 Reads

Virus survival and fitness when multiple genotypes and subtypes of influenza A viruses exist and circulate in swine.

Virology 2019 Apr 9;532:30-38. Epub 2019 Apr 9.

Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS, USA. Electronic address:

We performed swine influenza virus (SIV) surveillance in Midwest USA and isolated 100 SIVs including endemic and reassortant H1 and H3 viruses with 2009 pandemic H1N1 genes. To determine virus evolution when different genotypes and subtypes of influenza A viruses circulating in the same swine herd, a virus survival experiment was conducted in pigs mimicking field situations. Five different SIVs were used to infect five pigs individually, then two groups of sentinel pigs were introduced to investigate virus transmission. Read More

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http://dx.doi.org/10.1016/j.virol.2019.03.016DOI Listing

Electroconvulsive therapy "corrects" the neural architecture of visuospatial memory: Implications for typical cognitive-affective functioning.

Neuroimage Clin 2019 Apr 5;23:101816. Epub 2019 Apr 5.

Rotman Research Institute, University of Toronto, Canada. Electronic address:

Although electroconvulsive therapy (ECT) is a widely used and effective treatment for refractory depression, the neural underpinnings of its therapeutic effects remain poorly understood. To address this issue, here, we focused on a core cognitive deficit associated with depression, which tends to be reliably ameliorated through ECT, specifically, the ability to learn visuospatial information. Thus, we pursued three goals. Read More

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http://dx.doi.org/10.1016/j.nicl.2019.101816DOI Listing
April 2019
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Age-dependent variations in the expression of myosin isoforms and myogenic factors during the involution of the proximal sesamoidean ligament of sheep.

Res Vet Sci 2019 Apr 11;124:270-279. Epub 2019 Apr 11.

Department of Comparative Biomedicine and Food Science, Università di Padova, Italy. Electronic address:

In ungulates the stability of the fetlock joint is dependent on several muscles, which are exposed to high stress and strain. Among those muscles, the proximal sesamoidean ligament or PSL (also known as the suspensory ligament or Ruini's elasto-tendinous organ) is organized at birth in layers of muscle fibres alternated with abundant tendinous tissue that, during the postnatal development, becomes the predominant tissue. In this study we analysed the PSL of the sheep at the age of 1, 30 and 180 days and determined the expression of several genes which either (a) are markers of muscle fibre growth and maturation, or (b) play a role as signal molecules. Read More

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http://dx.doi.org/10.1016/j.rvsc.2019.04.006DOI Listing

Genetic factors and therapy outcomes in persistent developmental stuttering.

J Commun Disord 2019 Apr 5;80:11-17. Epub 2019 Apr 5.

National Institute on Deafness and Other Communication Disorders, National Institutes of Health, 35A Convent Drive, Bethesda, MD 20892, USA. Electronic address:

Purpose: We investigated whether outcomes of therapy for persistent developmental stuttering differ in individuals who carry a mutation in one of the known genes associated with stuttering compared to individuals without such mutations.

Method: We studied outcomes of an intensive fluency shaping-based therapy program in individuals with persistent developmental stuttering. We evaluated a cohort of 51 stuttering individuals with who carried a mutation in either the GNPTAB, GNPTG, NAGPA, or AP4E1 gene. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00219924183016
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http://dx.doi.org/10.1016/j.jcomdis.2019.03.007DOI Listing
April 2019
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Natural Variations at TIG1 Encoding a TCP Transcription Factor Contribute to Plant Architecture Domestication in Rice.

Mol Plant 2019 Apr 16. Epub 2019 Apr 16.

State Key Laboratory of Plant Physiology and Biochemistry, China Agricultural University, Beijing, 100193, China. Electronic address:

The modification of plant architecture was a crucial target in rice domestication and modern genetic improvement. Although a fraction of genes regulating rice plant architecture have been characterized, the molecular mechanisms underlying rice plant architecture domestication has not been elucidated. Here we show that the phenotype of inclined growth tiller in wild rice is controlled by a single dominant gene, TILLER INCLINED GROWTH 1 (TIG1), which is on chromosome 8 and encodes a TCP transcriptional activator. Read More

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http://dx.doi.org/10.1016/j.molp.2019.04.005DOI Listing

PM2.5 induces EMT and promotes CSC properties by activating Notch pathway in vivo and vitro.

Ecotoxicol Environ Saf 2019 Apr 16;178:159-167. Epub 2019 Apr 16.

Department of Respiratory and Critical Care Medicine, Peking University First Hospital, Beijing, China. Electronic address:

Fine particulate matter (PM2.5) has been closely linked to increased morbidity and mortality of lung cancer worldwide. However, the role of PM2. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01476513193035
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http://dx.doi.org/10.1016/j.ecoenv.2019.03.086DOI Listing
April 2019
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Comparative transcriptome analyses reveal changes of gene expression in fresh and cryopreserved yellow catfish (Pelteobagrus fulvidraco) sperm and the effects of Cryoprotectant MeSO.

Int J Biol Macromol 2019 Apr 16. Epub 2019 Apr 16.

School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong, China. Electronic address:

This study, for the first time in fish, compared the transcriptome of fresh and frozen-thawed sperm, and would help to better understand the effect of cryopreservation on fish sperm and then better preserve the aquatic germplasm resources. Here, we employed high-throughput sequencing technology to obtain the transcriptome of yellow catfish from fresh sperm, cryopreserved sperm with and without cryoprotectant. When cryoprotectant (MeSO) was excluded, down-regulated genes were significantly enriched into calcium ion binding, cytoskeletal protein binding, microfilament motor activity and carnitine O-acyltransferase activity, which affected Ca regulation, cellular morphology, motility and metabolism. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2019.04.050DOI Listing

Broad domains of histone 3 lysine 4 trimethylation are associated with transcriptional activation in CA1 neurons of the hippocampus during memory formation.

Neurobiol Learn Mem 2019 Apr 16. Epub 2019 Apr 16.

Vanderbilt Brain Institute, Vanderbilt University, Nashville, TN, USA. Electronic address:

Transcriptional changes in the hippocampus are required for memory formation, and these changes are regulated by numerous post-translational modifications of chromatin-associated proteins. One of the epigenetic marks that has been implicated in memory formation is histone 3 lysine 4 trimethylation (H3K4me3), and this modification is found at the promoters of actively transcribed genes. The total levels of H3K4me3 are increased in the CA1 region of the hippocampus during memory formation, and genetic perturbation of the K4 methyltransferases and demethylases interferes with forming memories. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10747427193007
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http://dx.doi.org/10.1016/j.nlm.2019.04.009DOI Listing
April 2019
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The association between alcohol metabolism and genetic variants of ADH1A, SRPRB, and PGM1 in Korea.

Alcohol 2019 Apr 16. Epub 2019 Apr 16.

Division of Endocrine and Metabolic Disease, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju, Chungbuk, 28159, South Korea.

Background: Excessive alcohol consumption is a major public health problem in East Asian countries. Alcohol use leads to a cascade of problems including increased chances of risky behavior and wide range of negative health consequences from alcoholic liver disease to upper gastric and liver cancer. These alcohol effects are known to be influenced by ethnic variability and genetics. Read More

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http://dx.doi.org/10.1016/j.alcohol.2019.03.004DOI Listing

Phylogenomics of the longitarsal Colossendeidae: The evolutionary history of an Antarctic sea spider radiation.

Mol Phylogenet Evol 2019 Apr 16. Epub 2019 Apr 16.

Zoological Research Museum Alexander Koenig, Statistical Phylogenetics and Phylogenomics, Adenauerallee 160, D-53113 Bonn, Germany; Faculty of Mathematics and Natural Sciences, University of Bonn, D-53012 Bonn, Germany.

Sea spiders (Pycnogonida) constitute a group of marine benthic arthropods that has a particularly high species diversity in the Southern Ocean. The "longitarsal" group of the sea spider family Colossendeidae is especially abundant in this region. However, this group also includes some representatives from other oceans, which raises the question where the group originates from. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10557903183051
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http://dx.doi.org/10.1016/j.ympev.2019.04.017DOI Listing
April 2019
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Immunity of Fleas (Order Siphonaptera).

Authors:
Lisa D Brown

Dev Comp Immunol 2019 Apr 16. Epub 2019 Apr 16.

Department of Biology, Georgia Southern University, P.O. Box 8042-1, Statesboro, GA, 30460, USA. Electronic address:

The immune response of arthropod vectors plays a key role in the spread and transmission of vector-borne diseases. Although fleas transmit several human pathogens (e.g. Read More

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http://dx.doi.org/10.1016/j.dci.2019.03.019DOI Listing

The RAG2 gene of yellow catfish (Tachysurus fulvidraco) and its immune response against Edwardsiella ictaluri infection.

Dev Comp Immunol 2019 Apr 16. Epub 2019 Apr 16.

Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, 510225, China. Electronic address:

Recombination-activating gene 2 (rag 2) allies with recombination-activating gene 1 (rag 1) and regulates the V(D)J recombination of immunoglobulin (Ig) and T-cell receptor (TCR) genes. Being a key player in the adaptive immune response of vertebrates, functional characterization of rag 2 from yellow catfish is beneficial for understanding the biological response towards the pathogens. In this report, we have cloned and characterized the rag 2 gene of yellow catfish, and a particular pattern of expression was analysed in the major tissues of yellow catfish. Read More

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http://dx.doi.org/10.1016/j.dci.2019.04.006DOI Listing

Metabolic and physiological perturbations of Escherichia coli W3100 by bacterial small RNA RyhB.

Biochimie 2019 Apr 16. Epub 2019 Apr 16.

Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, 230027, People's Republic of China. Electronic address:

RyhB is a key regulator of iron level in Escherichia coli (E. coli), which assists in conserving iron for life-sustaining cellular functions when cytoplasmic levels of the ferrous form of iron is limited. RyhB affects glucose metabolism. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.04.016DOI Listing

Effect of estradiol on hepatopancreatic lipid metabolism in the swimming crab, Portunus trituberculatus.

Gen Comp Endocrinol 2019 Apr 16. Epub 2019 Apr 16.

Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China; Centre for Research on Environmental Ecology and Fish Nutrition of Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; National Demonstration Centre for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China. Electronic address:

Estradiol is an important sex steroid hormone that involved in regulation of animal lipid metabolism. However, the effect of estradiol on lipid metabolism in swimming crab (Portunus trituberculatus) is unclear. The present study investigated the effect of four concentrations of exogenous estradiol (0, 0. Read More

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http://dx.doi.org/10.1016/j.ygcen.2019.04.019DOI Listing

Functional analysis of miR-21-3p, miR-30b-5p and miR-150-5p shuttled by extracellular vesicles from diabetic subjects reveals their association with diabetic retinopathy.

Exp Eye Res 2019 Apr 16. Epub 2019 Apr 16.

Dept of Medical Sciences, University of Turin, Italy. Electronic address:

Microvascular dysfunctions due to altered interactions between endothelial cells (ECs) and pericytes are key-events in the pathogenesis of diabetic retinopathy. Extracellular vesicles (EVs) derived from mesenchymal stem cells cultured in diabetic-like conditions enter pericytes, cause their detachment and migration, and stimulate angiogenesis. We recently showed that EVs from diabetic patients with retinopathy have different miRNA profiling patterns from healthy controls, and determine features of retinopathy in in vitro models of retinal microvasculature. Read More

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http://dx.doi.org/10.1016/j.exer.2019.04.015DOI Listing

Activation of P-TEFb by RBM7: To Live or Let Die.

Mol Cell 2019 Apr;74(2):223-224

IGBMC (CNRS, INSERM, UdS), 1 rue Laurent Fries, 67400 Illkirch Graffenstaden, France. Electronic address:

In this issue of Molecular Cell, Bugai et al. (2019) unveil that a key step of the pro-survival cellular response to a genotoxic attack is the activation of P-TEFb by RBM7. This crucial step triggers RNA polymerase II release from promoter-proximal pausing and expression of DNA damage response genes. Read More

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http://dx.doi.org/10.1016/j.molcel.2019.03.039DOI Listing

Protein Sequence Editing of SKN-1A/Nrf1 by Peptide:N-Glycanase Controls Proteasome Gene Expression.

Cell 2019 Apr;177(3):737-750.e15

Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA; Department of Genetics, Harvard Medical School, Boston, MA 02115, USA. Electronic address:

The proteasome mediates selective protein degradation and is dynamically regulated in response to proteotoxic challenges. SKN-1A/Nrf1, an endoplasmic reticulum (ER)-associated transcription factor that undergoes N-linked glycosylation, serves as a sensor of proteasome dysfunction and triggers compensatory upregulation of proteasome subunit genes. Here, we show that the PNG-1/NGLY1 peptide:N-glycanase edits the sequence of SKN-1A protein by converting particular N-glycosylated asparagine residues to aspartic acid. Read More

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http://dx.doi.org/10.1016/j.cell.2019.03.035DOI Listing

Core catalysis of the reductive glycine pathway demonstrated in yeast.

ACS Synth Biol 2019 Apr 19. Epub 2019 Apr 19.

One-carbon (C1) compounds are attractive microbial feedstocks as they can be efficiently produced from widely available resources. Formate, in particular, represents a promising growth substrate, as it can be generated from electrochemical reduction of CO2 and fed to microorganisms in a soluble form. We previously identified the synthetic reductive glycine pathway as the most efficient route for aerobic growth on formate. Read More

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http://pubs.acs.org/doi/10.1021/acssynbio.8b00464
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http://dx.doi.org/10.1021/acssynbio.8b00464DOI Listing
April 2019
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Semen quality is affected by HLA class I alleles together with sexually transmitted diseases.

Andrology 2019 Apr 19. Epub 2019 Apr 19.

Instituto de Investigação e Inovação em Saúde, Universidade do Porto (i3S), Porto, Portugal.

Background: The human leukocyte antigen (HLA) locus includes several genes with key roles in antigen presentation and immune response, some of them inclusively found to be associated with non-obstructive azoospermia. Still, HLA connections to other infertility phenotypes such as semen hyperviscosity (SHV), asthenozoospermia (AST), and oligozoospermia (OLI) have been often neglected.

Objectives: In this work, we aimed to evaluate the association of HLA class I and II genes with SHV, AST, and OLI phenotypes while exploring a possible role in an adaptive immune response to sexually transmitted diseases (STD). Read More

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http://dx.doi.org/10.1111/andr.12625DOI Listing

Niche-specific metabolic adaptation in biotrophic and necrotrophic oomycetes is manifested in differential use of nutrients, variation in gene content, and enzyme evolution.

PLoS Pathog 2019 Apr 19;15(4):e1007729. Epub 2019 Apr 19.

Department of Microbiology and Plant Pathology, University of California, Riverside United States of America.

The use of host nutrients to support pathogen growth is central to disease. We addressed the relationship between metabolism and trophic behavior by comparing metabolic gene expression during potato tuber colonization by two oomycetes, the hemibiotroph Phytophthora infestans and the necrotroph Pythium ultimum. Genes for several pathways including amino acid, nucleotide, and cofactor biosynthesis were expressed more by Ph. Read More

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http://dx.doi.org/10.1371/journal.ppat.1007729DOI Listing

Single-cell correlations of mRNA and protein content in a human monocytic cell line after LPS stimulation.

PLoS One 2019 19;14(4):e0215602. Epub 2019 Apr 19.

Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico, United States of America.

The heterogeneity of mRNA and protein expression at the single-cell level can reveal fundamental information about cellular response to external stimuli, including the sensitivity, timing, and regulatory interactions of genes. Here we describe a fully automated system to digitally count the intron, mRNA, and protein content of up to five genes of interest simultaneously in single-cells. Full system automation of 3D microscope scans and custom image analysis routines allows hundreds of individual cells to be automatically segmented and the mRNA-protein content to be digitally counted. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215602PLOS

Preclinical evaluation of EPHX2 inhibition as a novel treatment for inflammatory bowel disease.

PLoS One 2019 19;14(4):e0215033. Epub 2019 Apr 19.

Computational Biology, GlaxoSmithKline, Collegeville, Pennsylvania, United States of America.

Epoxyeicosatrienoic acids (EETs) are signaling lipids produced by cytochrome P450 epoxygenation of arachidonic acid, which are metabolized by EPHX2 (epoxide hydrolase 2, alias soluble epoxide hydrolase or sEH). EETs have pleiotropic effects, including anti-inflammatory activity. Using a Connectivity Map (CMAP) approach, we identified an inverse-correlation between an exemplar EPHX2 inhibitor (EPHX2i) compound response and an inflammatory bowel disease patient-derived signature. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215033PLOS

Identification of cotton MOTHER OF FT AND TFL1 homologs, GhMFT1 and GhMFT2, involved in seed germination.

PLoS One 2019 19;14(4):e0215771. Epub 2019 Apr 19.

Special Plant Genomics Laboratory, College of Life Sciences, Shihezi University, Shihezi, Xinjiang, China.

Plant phosphatidylethanolamine-binding protein (PEBP) is comprised of three clades: FLOWERING LOCUS T (FT), TERMINAL FLOWER1 (TFL1) and MOTHER OF FT AND TFL1 (MFT). FT/TFL1-like clades regulate identities of the determinate and indeterminate meristems, and ultimately affect flowering time and plant architecture. MFT is generally considered to be the ancestor of FT/TFL1, but its function is not well understood. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215771PLOS

Epigenetic regulator G9a provides glucose as a sweet key to stress resistance.

PLoS Biol 2019 Apr 19;17(4):e3000236. Epub 2019 Apr 19.

Centre for Molecular Medicine and Therapeutics, BC Children's Hospital Research Institute, Department of Medical Genetics, The University of British Columbia, Vancouver, British Columbia, Canada.

The ability to adapt to acute and chronic stress is important for organisms to thrive in evolutionary niches and for cells to survive in adverse conditions. The regulatory networks that control stress responses are evolutionarily conserved, and many factors that selectively activate stress responses have been identified. Less well understood are mechanisms that guard against unnecessary induction of cytoprotective factors and that connect stress responses with cellular metabolism to control energy expenditure during stress. Read More

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http://dx.doi.org/10.1371/journal.pbio.3000236DOI Listing

Identification and Evaluation of Reference Genes for qRT-PCR Normalization in Sparassis latifolia (Agaricomycetes).

Int J Med Mushrooms 2019 ;21(3):301-309

Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, China; National and Local Joint Engineering Research Center for Breeding and Cultivation of Featured Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, China.

Real-time quantitative polymerase chain reaction (qRT-PCR) has emerged as a powerful and popular tool for quantitating differences in transcriptional gene expression levels between samples. Validation of the stability of reference genes is a fundamental step before initiating qRT-PCR assays. Sparassis latifolia is an edible and medicinal fungus containing a remarkably high concentration of β-glucan, which has many biological and pharmacologic activities. Read More

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http://dx.doi.org/10.1615/IntJMedMushrooms.2019030106DOI Listing
January 2019

Gene Delivery Using Lipoplexes and Polyplexes: Principles, Limitations and Solutions.

Crit Rev Eukaryot Gene Expr 2019 ;29(1):29-36

Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.

Gene therapy has attracted considerable attention for the treatment of genetic and acquired diseases. Successful gene therapy occurs when the therapeutic genes penetrate targeted cells and become available to the intracellular active site. Currently, a promising approach in gene delivery is the use of nonviral gene delivery vectors that lack immunogenicity but have low toxicity and potential tissue specificity. Read More

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http://dx.doi.org/10.1615/CritRevEukaryotGeneExpr.2018025132DOI Listing
January 2019

Arrdc2 and Arrdc3 Elicit Divergent Changes in Gene Expression in Skeletal Muscle following Anabolic and Catabolic Stimuli.

Physiol Genomics 2019 Apr 19. Epub 2019 Apr 19.

Rancho BioSciences, United States.

Skeletal muscle is a highly plastic organ regulating various processes in the body. As such, loss of skeletal muscle underlies the increased morbidity and mortality risk that is associated with numerous conditions. However, no therapies are available to combat the loss of muscle mass during atrophic conditions, which is due in part to the incomplete understanding of the molecular networks altered by anabolic and catabolic stimuli. Read More

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https://www.physiology.org/doi/10.1152/physiolgenomics.00007
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http://dx.doi.org/10.1152/physiolgenomics.00007.2019DOI Listing
April 2019
2 Reads

Advanced Epithelial Ovarian Cancer: Do More Options Mean Greater Benefits?

J Clin Oncol 2019 Apr 19:JCO1900500. Epub 2019 Apr 19.

1 Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA.

A healthy 51-year-old woman presented with increasing abdominal and pelvic pain. Computed tomography imaging of the abdomen and pelvis showed an 11.6-cm pelvic mass, retroperitoneal lymphadenopathy, right hydronephrosis, and mesenteric tumor deposits ( Fig 1A ). Read More

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http://dx.doi.org/10.1200/JCO.19.00500DOI Listing

The circadian clock gene is required to control the timing of retinal neurogenesis and lamination of Müller glia in the mouse retina.

FASEB J 2019 Apr 19:fj201801832RR. Epub 2019 Apr 19.

Department of Ophthalmic Research, Cole Eye Institute, Cleveland Clinic, Cleveland, Ohio, USA.

A single pool of multipotent retinal progenitor cells give rise to the diverse cell types within the mammalian retina. Such cellular diversity is due to precise control of various cellular processes like cell specification, proliferation, differentiation, and maturation. Circadian clock genes can control the expression of key regulators of cell cycle progression and therefore can synchronize the cell cycle state of a heterogeneous population of cells. Read More

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http://dx.doi.org/10.1096/fj.201801832RRDOI Listing

Upregulation of MicroRNA-21 promotes tumorigenesis of prostate cancer cells by targeting KLF5.

Cancer Biol Ther 2019 Apr 19:1-13. Epub 2019 Apr 19.

a Key Laboratory of Laboratory Medicine, Ministry of Education of China, Zhejiang Provincial Key Laboratory of Medical Genetics, School of Laboratory Medicine and Life Sciences , Wenzhou Medical University , Wenzhou , Zhejiang , China.

Prostate cancer (PCa) is the second frequently newly diagnosed cancer in men. Androgen deprivation therapy has been widely used to inhibit PCa growth but eventually fails in many patients. Androgen receptor and its downstream molecules like microRNAs could be promising therapeutic targets. Read More

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http://dx.doi.org/10.1080/15384047.2019.1599659DOI Listing

Colonic dilation and altered ex vivo gastrointestinal motility in the neuroligin-3 knockout mouse.

Autism Res 2019 Apr 19. Epub 2019 Apr 19.

Department of Physiology, University of Melbourne, Melbourne, Victoria, Australia.

Gastrointestinal (GI) dysfunction is commonly reported by people diagnosed with autism spectrum disorder (ASD; autism) but the cause is unknown. Mutations in genes encoding synaptic proteins including Neuroligin-3 are associated with autism. Mice lacking Neuroligin-3 (Nlgn3 ) have altered brain function, but whether the enteric nervous system (ENS) is altered remains unknown. Read More

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http://dx.doi.org/10.1002/aur.2109DOI Listing

Free fatty acid receptor GPR120 and GPR40 are essential for oil-induced GIP secretion.

J Diabetes Investig 2019 Apr 19. Epub 2019 Apr 19.

Department of Diabetes, Endocrinology and Nutrition, Graduate School of Medicine, Kyoto University.

Aims/introduction: Incretin hormone glucose-dependent insulinotropic polypeptide / gastric inhibitory polypeptide (GIP) plays a key role in high-fat diet-induced obesity and insulin resistance. GIP is strongly secreted from enteroendocrine K cells by oil ingestion. GPR120 and GPR40 are two major receptors for long chain fatty acids and are expressed in enteroendocrine K cells. Read More

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http://dx.doi.org/10.1111/jdi.13059DOI Listing

The Cystathionine beta-lyase is crucial for embryo patterning and the maintenance of root stem cell niche in Arabidopsis.

Plant J 2019 Apr 19. Epub 2019 Apr 19.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, China.

The growth and development of roots in plants depends on the specification and maintenance of the root apical meristem. Here, we report the identification of CBL, a gene required for embryo and root development in Arabidopsis, and encodes cystathionine beta-lyase (CBL) that catalyzes the penultimate step in methionine (Met) biosynthesis, which also led to the elucidation of a previous unknown but crucial metabolic contribution by the Met biosynthesis pathway. CBL is expressed in embryos and showing quiescent center (QC)-enriched expression pattern in the root. Read More

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http://dx.doi.org/10.1111/tpj.14343DOI Listing

Enzymatic basis for C-lignin monomer biosynthesis in the seed coat of Cleome hassleriana.

Plant J 2019 Apr 19. Epub 2019 Apr 19.

BioDiscovery Institute, University of North Texas, Denton, TX.

C-lignin is a linear polymer of caffeyl alcohol, found in the seed coats of several exotic plant species, with promising properties for generation of carbon fibers and high value chemicals. In the ornamental plant Cleome hassleriana, guaiacyl (G) lignin is deposited in the seed coat for the first 6-12 days after pollination, after which G lignin deposition ceases and C-lignin accumulates, providing an excellent model system to study C-lignin biosynthesis. We performed RNA sequencing of seed coats harvested at 2-day intervals throughout development. Read More

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http://dx.doi.org/10.1111/tpj.14340DOI Listing

CRISPR/Cas9-mediated functional recovery of the recessive rc allele to develop red rice.

Plant Biotechnol J 2019 Apr 19. Epub 2019 Apr 19.

Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, China.

Red rice contains high levels of proanthocyanidins and anthocyanins, which have been recognized as health-promoting nutrients. The red colouration of rice grains is controlled by two complementary genes, Rc and Rd. The RcRd genotype produces red pericarp in wild species Oryza rufipogon, whereas most cultivated rice varieties produce white grains resulted from a 14-bp frame-shift deletion in the seventh exon of the Rc gene. Read More

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http://dx.doi.org/10.1111/pbi.13125DOI Listing

Nitrogen utilization and metabolism in maize (Zea mays L.) plants under different rates of biochar addition and nitrogen input conditions.

Plant Biol (Stuttg) 2019 Apr 19. Epub 2019 Apr 19.

Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning Province, China.

Biochar (BC) application could improve plant nitrogen (N) utilization and potentially reduce N fertilizer requirements. However, the fate of N in crop-soil systems and the metabolic responses of crops under the condition of BC coapplied with reduced N are not well understood. The urea- N isotope and pot experiments with three BC rates (0%, 5% and 15%; w/w) combined with three N fertilizer levels (100%-N, 85%-N and 55%-N) were conducted for maize. Read More

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http://dx.doi.org/10.1111/plb.12997DOI Listing

Antimicrobial activity of Mimosa caesalpiniifolia Benth and its interaction with antibiotics against Staphylococcus aureus strains overexpressing efflux pump genes.

Lett Appl Microbiol 2019 Apr 19. Epub 2019 Apr 19.

Laboratory of Research in Microbiology, Federal University of Piauí, Teresina, PI, Brazil.

This study aimed to evaluate the antimicrobial activity of the dichloromethane fraction (DCMF) from the stem bark of Mimosa caesalpiniifolia and its effect on the activity of conventional antibiotics against S. aureus strains overexpressing specific efflux pump genes. DCMF showed activity against S. Read More

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http://dx.doi.org/10.1111/lam.13163DOI Listing

Regulation of the Unfolded Protein Response in Yeast by Oxidative Stress.

FEBS Lett 2019 Apr 19. Epub 2019 Apr 19.

Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, United States of America.

In the unfolded protein response (UPR), Ire1 activates Hac1 to coordinate the transcription of hundreds of genes to mitigate ER stress. Recent work in C. elegans suggests that oxidative stress inhibits this canonical Ire1 signaling pathway, activating instead an anti-oxidant stress response. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/1873-3468.13
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http://dx.doi.org/10.1002/1873-3468.13389DOI Listing
April 2019
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Single‑cell intratumoral stemness analysis reveals the involvement of cell cycle and DNA damage repair in two different types of esophageal cancer.

Oncol Rep 2019 Apr 15. Epub 2019 Apr 15.

Cancer Research Institute, Hangzhou Cancer Hospital, Hangzhou, Zhejiang 320000, P.R. China.

Intratumoral heterogeneity, particularly the potential cancer stemness of single cancer cells, has not yet been fully elucidated in human esophageal cancer. Single‑cell transcriptome sequencing of two types of esophageal adenocarcinoma (EAC) and two types of esophageal squamous cell carcinoma (ESCC) tissues was performed, and the intratumoral cancer stemness of the types of esophageal cancer were characterized at the single‑cell level in the present study. By comparing the transcriptomic profiles of single cancer cells with high and low stemness in individual patients, it was revealed that the overexpression of cell cycle‑associated genes in EAC cells was highly correlated with stemness, whereas overexpression of genes involved in the signaling pathways of DNA replication and DNA damage repair was significantly correlated with stemness in ESCC. Read More

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http://www.spandidos-publications.com/10.3892/or.2019.7117
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http://dx.doi.org/10.3892/or.2019.7117DOI Listing
April 2019
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A 19‑miRNA Support Vector Machine classifier and a 6‑miRNA risk score system designed for ovarian cancer patients.

Oncol Rep 2019 Apr 10. Epub 2019 Apr 10.

Department of Anesthesiology, Beijing Obstetrics and Gynecology Hospital, Dongcheng, Beijing 100001, P.R. China.

Ovarian cancer (OC) is the most common gynecologic malignancy with high incidence and mortality. The present study aimed to develop approaches for determining the recurrence type and identify potential miRNA markers for OC prognosis. The miRNA expression profile of OC (the training set, including 390 samples with recurrence information) was downloaded from The Cancer Genome Atlas database. Read More

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http://dx.doi.org/10.3892/or.2019.7108DOI Listing

Methylation of the promoter region of the MTRR gene in childhood acute lymphoblastic leukemia.

Oncol Rep 2019 Apr 12. Epub 2019 Apr 12.

Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Lanzhou, Gansu 730030, P.R. China.

Epigenetic analysis of the association between the methylation status of the promoter region of the MTRR (5‑methyltetrahydrofolate‑homocysteine methyltransferase reductase) gene and the risk of acute lymphoblastic leukemia (ALL) in children plays an important role in the early diagnosis, assessment of the malignant degree, treatment and evaluation of the risk of relapse and prognosis of the disease. In the present study, RT‑qPCR was used to detect the mRNA levels of the MTRR and MTHFR (methylenetetrahydrofolate reductase) genes in the bone marrow of 20 ALL patients and 20 age‑ and sex‑matched controls with normal bone marrow. The methylation pattern of the MTRR promoter region in eligible DNA samples was quantitatively analyzed using MALDI‑TOF MS. Read More

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http://www.spandidos-publications.com/10.3892/or.2019.7114
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http://dx.doi.org/10.3892/or.2019.7114DOI Listing
April 2019
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DNA methylation of metallothionein genes is associated with the clinical features of renal cell carcinoma.

Oncol Rep 2019 Apr 10. Epub 2019 Apr 10.

Institute of Biosciences, Life Sciences Center, Vilnius University, LT‑10257 Vilnius, Lithuania.

Metallothioneins are low‑weight cysteine‑rich proteins responsible for metal ion homeostasis in a cell and, thus, capable of regulating cell proliferation and differentiation. Deregulation of metallothionein genes has been reported in various human tumors. However, their role in renal cell carcinoma (RCC) has been poorly investigated. Read More

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http://dx.doi.org/10.3892/or.2019.7109DOI Listing

The use of aminoglycosides in animals within the EU: development of resistance in animals and possible impact on human and animal health: a review.

J Antimicrob Chemother 2019 Apr 19. Epub 2019 Apr 19.

Veterinary Medicines Directorate, Addlestone, UK.

Aminoglycosides (AGs) are important antibacterial agents for the treatment of various infections in humans and animals. Following extensive use of AGs in humans, food-producing animals and companion animals, acquired resistance among human and animal pathogens and commensal bacteria has emerged. Acquired resistance occurs through several mechanisms, but enzymatic inactivation of AGs is the most common one. Read More

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http://dx.doi.org/10.1093/jac/dkz161DOI Listing

Longitudinal Muscle and Myocellular Changes in Community-Dwelling Men Over Two Decades of Successful Aging-The ULSAM Cohort Revisited.

J Gerontol A Biol Sci Med Sci 2019 Apr 19. Epub 2019 Apr 19.

Department of Laboratory Medicine, Division of Clinical Physiology, Karolinska Institutet, and Unit of Clinical Physiology, Karolinska University Hospital, Stockholm, Sweden.

Participants of the population-based Uppsala longitudinal study of adult men (ULSAM) cohort reaching more than 88 years of age (survivors, S) were investigated at age 70, 82, and 88-90 and compared at 70 years with non-survivors (NS) not reaching 82 years. Body composition, muscle mass and muscle histology were remarkably stable over 18 years of advanced aging in S. Analysis of genes involved in muscle remodeling showed that S had higher mRNA levels of myogenic differentiation factors (Myogenin, MyoD), embryonic myosin (eMyHC), enzymes involved in regulated breakdown of myofibrillar proteins (Smad2, Trim32, MuRF1,) and NCAM compared with healthy adult men (n = 8). Read More

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http://dx.doi.org/10.1093/gerona/glz068DOI Listing
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