2,534,890 results match your criteria Gene Therapy & Molecular Biology [Journal]


[Genetic profiling in the diagnosis of hereditary prostate cancer: Where do we stand?]

Aktuelle Urol 2018 Dec 6;49(6):525-529. Epub 2018 Dec 6.

Johannes Gutenberg Universitat Universitatsmedizin, Klinik und Poliklinik für Urologie und Kinderurologie, Mainz.

Prostate cancer has a heterogeneous genetic profile compared with other tumour entities. Accordingly, there are also various mutations that increase the risk of prostate cancer. Some genetic variants only have a mild impact, whereas other gene mutations (BRCA1 /2; HOXB13) may increase the risk significantly. Read More

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December 2018
5 Reads

Migraine and Tension-Type Headache.

Semin Neurol 2018 Dec 6;38(6):608-618. Epub 2018 Dec 6.

Department of Neurology, Boston Medical Center, Boston, Massachusetts.

Migraine and tension-type headache (TTH) are common primary disorders that carry significant morbidity and socioeconomic effect. In this article, we will review the epidemiology, presentation, and diagnosis of these disorders. First-line acute treatment for migraine consists of analgesics, triptans, and antiemetics, while nonsteroidal anti-inflammatory drugs are the mainstay treatment for TTH. Read More

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December 2018
2 Reads

Microbial Intervention as a Novel Target in Treatment of Non-Alcoholic Fatty Liver Disease Progression.

Cell Physiol Biochem 2018 Dec 6;51(5):2123-2135. Epub 2018 Dec 6.

Department of Gastroenterology, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, the Second Affiliated Hospital of South China University of Technology, Guangzhou,

Background/aims: Emerging evidence suggests a close link between gut microbiota and non-alcoholic fatty liver disease (NAFLD). In this study, we aimed to investigate the association between gut microbiota and the DNA methylation of adiponectin (an adipocyte-specific adipocytokine) in rats, following diet-induced NAFLD.

Methods: 50 male SD rats were randomly divided into five groups with or without a high fat diet (HFD), antibiotics, and probiotics, in order to establish an imbalanced gut microbiota and probiotic treatment model in NAFLD rats. Read More

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December 2018
2 Reads

Downregulation of microRNA-376a in Gastric Cancer and Association with Poor Prognosis.

Cell Physiol Biochem 2018 Dec 6;51(5):2010-2018. Epub 2018 Dec 6.

Background/aims: MicroRNAs have a significant role in the tumorigenesis and progression of cancers, including gastric cancer (GC). Our study aimed to identify a novel biomarker to predict the prognosis of patients with GC.

Methods: The GC microarray dataset, GSE28700, was downloaded from the Gene Expression Omnibus (GEO) database and screened for differentially expressed miRNAs (DEMs). Read More

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December 2018
2 Reads

Development and Application of a New PCR Method for Detection of Blumeria graminis f. sp. tritici.

J Mol Microbiol Biotechnol 2018 Dec 6;28(3):137-146. Epub 2018 Dec 6.

Department of Biotechnology, Microbiology and Human Nutrition, University of Life Sciences in Lublin, Lublin, Poland.

We developed new PCR assays that target beta-tubulin (TUB2) and 14 alpha-demethylase (CYP51) genes and used them for the species-specific detection of Blumeria graminis f. sp. tritici (Bgt). Read More

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December 2018
3 Reads

Gastric Adenocarcinoma Predictive Long Intergenic Non-Coding RNA Promotes Tumor Occurrence and Progression in Non-Small Cell Lung Cancer via Regulation of the miR-661/eEF2K Signaling Pathway.

Cell Physiol Biochem 2018 Dec 6;51(5):2136-2147. Epub 2018 Dec 6.

Background/aims: Long non-coding RNAs (lncRNAs) play vital roles in carcinogenesis as oncogenes or tumor suppressor genes. This study explored the biological function of lncRNA gastric adenocarcinoma predictive long intergenic non-coding RNA (GAPLINC) in human non-small cell lung cancer (NSCLC).

Methods: GAPLINC expression in NSCLC specimens and cell lines was detected by qRT-PCR and Western blot. Read More

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December 2018
2 Reads

Taxifolin Inhibits Receptor Activator of NF-κB Ligand-Induced Osteoclastogenesis of Human Bone Marrow-Derived Macrophages in vitro and Prevents Lipopolysaccharide-Induced Bone Loss in vivo.

Pharmacology 2018 Dec 6;103(1-2):101-109. Epub 2018 Dec 6.

It has been reported that taxifolin inhibit osteoclastogenesis in RAW264.7 cells. In our research, the inhibition effects of taxifolin on the osteoclastogenesis of human bone marrow-derived macrophages (BMMs) induced by receptor activator of NF-κB ligand (RANKL) as well as the protection effects in lipopolysaccharide-induced bone lysis mouse model have been demonstrated. Read More

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December 2018
3 Reads

miR-202 Enhances the Anti-Tumor Effect of Cisplatin on Non-Small Cell Lung Cancer by Targeting the Ras/MAPK Pathway.

Cell Physiol Biochem 2018 Dec 6;51(5):2160-2171. Epub 2018 Dec 6.

Background/aims: KRas is usually mutated in non-small cell lung cancer (NSCLC). The mutated KRas gene is a negative prognostic indicator that promotes tumor proliferation, metastasis, and drug resistance in NSCLC, and thus has become a target for cancer therapy. This study is focused on the effects of the microRNA (miR)-202/KRas axis in regulating chemosensitivity in NSCLC. Read More

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December 2018
1 Read

Clinical Drug Response Prediction by Using a Lq Penalized Network-Constrained Logistic Regression Method.

Cell Physiol Biochem 2018 Dec 6;51(5):2073-2084. Epub 2018 Dec 6.

Faculty of Information Technology & State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China.

Background/aims: One of the most important impacts of personalized medicine is the connection between patients' genotypes and their drug responses. Despite a series of studies exploring this relationship, the predictive ability of such analyses still needs to be strengthened.

Methods: Here we present the Lq penalized network-constrained logistic regression (Lq-NLR) method to meet this need, in which the predictors are integrated into the gene expression data and biological network knowledge and are combined with a more aggressive penalty function. Read More

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December 2018
2 Reads

Impact of eutrophication on arsenic cycling in freshwaters.

Water Res 2018 Nov 21;150:191-199. Epub 2018 Nov 21.

Key Laboratory of Environmental Nanotechnology and Health Effects, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, PR China; Centre of Integrated Water-Energy-Food Studies (iWEF), School of Animal, Rural, and Environmental Sciences, Nottingham Trent University, Brackenhurst Campus, NG25 0QF, UK. Electronic address:

Many arsenic-bearing freshwaters are facing with eutrophication and consequent algae-induced anoxia/hypoxia events. However, arsenic cycling in eutrophic waters and its impact on public health are poorly understood. Laboratory simulation experiments are performed in this study to investigate the effect of algal blooms on the cycling of arsenic in a sediment-water-air system. Read More

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November 2018
4 Reads

Effects of ofloxacin on nitrogen removal and microbial community structure in constructed wetland.

Sci Total Environ 2018 Nov 27;656:503-511. Epub 2018 Nov 27.

Department of Civil and Environmental Engineering, National University of Singapore, 1 Engineering Drive 2, E1A 07-03, Singapore117576, Singapore.

Constructed wetlands (CWs) have emerged as a promising technology for the purification of micro-polluted water. However, their nitrogen removal performance can be significantly degraded by design, operational, and environmental factors. The present study investigates the effects of ofloxacin (OFL: 0. Read More

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November 2018
1 Read

Viability-based quantification of antibiotic resistance genes and human fecal markers in wastewater effluent and receiving waters.

Sci Total Environ 2018 Nov 22;656:495-502. Epub 2018 Nov 22.

Department of Civil and Environmental Engineering, Rutgers, The State University of New Jersey, 500 Bartholomew Dr, Piscataway, NJ 08854, United States. Electronic address:

Antibiotic resistance is a public health issue with links to environmental sources of antibiotic resistance genes (ARGs). ARGs from nonviable sources may pose a hazard given the potential for transformation whereas ARGs in viable sources may proliferate during host growth or conjugation. In this study, ARGs in the effluent from three municipal wastewater treatment plants (WWTPs) and the receiving surface waters were investigated using a viability-based qPCR technique (vPCR) with propidium monoazide (PMA). Read More

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November 2018
2 Reads

Er Shen Wan extract alleviates polyuria and regulates AQP 2 and AVPR 2 in a rat model of spleen-kidney Yang deficiency-induced diarrhea.

Biomed Pharmacother 2018 Dec 3;110:302-311. Epub 2018 Dec 3.

College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, PR China; Sichuan Neo-Green Pharmaceutical Technology Development Co., Ltd, Chengdu 610081, PR China. Electronic address:

Ethnopharmacological Relevance: Er Shen Wan (ESW), has been empirically used for treating spleen-kidney Yang deficiency (SKYD) syndrome in Traditional Chinese medicine (TCM) for centuries and shows a variety of activities. The medicinal formula is a mixture of two component herbs, Psoraleae Fructus (PF, Bu-Gu-Zhi in Chinese) and Myristicae Semen (MS, Rou-Dou-Kou in Chinese). The current study was designed to evaluate ESWP antidiuretic treatment of polyuria and to explore potential mechanisms of renal water metabolism in the rat model of SKYD-induced diarrhea. Read More

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December 2018
1 Read

Long non-coding RNA SNHG6 enhances cell proliferation, migration and invasion by regulating miR-26a-5p/MAPK6 in breast cancer.

Biomed Pharmacother 2018 Dec 3;110:294-301. Epub 2018 Dec 3.

Department of breast surgery, The first affiliated hospital of Zhengzhou University, Zhengzhou City, Henan Province, China.

The long non-coding RNA (lncRNA) has recently been shown to be important regulators involved in the progression of various human cancers. Small nucleolar RNA host gene 6 (SNHG6) is a recently identified cancer-related lncRNA. However, the clinical significance and biological function of SNHG6 in breast cancer (BC) are still unclear. Read More

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December 2018
2 Reads

Wuzhi capsule (Schisandra Sphenanthera extract) attenuates liver steatosis and inflammation during non-alcoholic fatty liver disease development.

Biomed Pharmacother 2018 Dec 3;110:285-293. Epub 2018 Dec 3.

Department of Organ Transplantation, ChangZheng Hospital, Second Military Medical University, Shanghai, 200003, China. Electronic address:

Objective: Wuzhi (WZ) capsule contains an ethanol extract of Schisandra sphenanthera. The efficacy of WZ in treating non-alcoholic fatty liver disease (NAFLD) has not yet been elucidated. The present study assessed the effects of WZ on NAFLD. Read More

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December 2018
2 Reads
2.023 Impact Factor

Temporal dynamics of cortisol-associated changes in mRNA expression of glucocorticoid responsive genes FKBP5, GILZ, SDPR, PER1, PER2 and PER3 in healthy humans.

Psychoneuroendocrinology 2018 Nov 30;102:63-67. Epub 2018 Nov 30.

Department of Neurobehavioral Genetics, Institute of Psychobiology, University of Trier, D-54290, Trier, Germany. Electronic address:

Secretion of the stress hormone cortisol follows a circadian rhythm and is stimulated following stress exposure. Cortisol regulates the transcription of several genes, primarily through activation of the glucocorticoid receptor (GR). Previously, we showed an upregulation of PERIOD genes PER1 and PER3 after pharmacological/glucocorticoid challenge in vivo and in vitro. Read More

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November 2018
1 Read

Epigenetics and meditation.

Authors:
Perla Kaliman

Curr Opin Psychol 2018 Nov 22;28:76-80. Epub 2018 Nov 22.

Faculty of Health Sciences, Universitat Oberta de Catalunya, Av. Tibidabo, 39-43, 08035, Barcelona, Spain; Honorary Fellow, Center for Healthy Minds, University of Wisconsin-Madison, United States. Electronic address:

In the last decade, epigenetics has taken center stage to explain the relationships between stress exposure, health and behavior. Acquired or inherited epigenetic changes modulate gene expression states without modifying the DNA sequence itself, they can be long-lasting, yet, they are potentially reversible. Several studies have explored whether meditation-based interventions can influence gene expression profiles towards healthier directions, identifying candidate genes and biological pathways that seem to be sensitive to contemplative practices. Read More

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November 2018
3 Reads

Combined juvenile polyposis syndrome and hereditary hemorrhagic telangiectasia (JPS/HHT) with MRI and endoscopic correlation.

Clin Imaging 2018 Dec 2;54:37-39. Epub 2018 Dec 2.

Department of Radiology, Beth Israel Deaconess Medical Center, 330 Brookline Ave., Boston, MA 02215, USA.

Juvenile polyposis syndrome (JPS) may coexist with hereditary hemorrhagic telangiectasia (HHT) due to implication of the SMAD4 gene in a subset of both diseases. To the best of our knowledge, we present the first case in the radiologic literature on the MRI findings in a patient with this rare combined diagnosis undergoing workup for burden of disease. Read More

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December 2018
2 Reads

Interferons Transcriptionally Up-Regulate MLKL Expression in Cancer Cells.

Neoplasia 2018 Dec 3;21(1):74-81. Epub 2018 Dec 3.

Institute for Experimental Cancer Research in Pediatrics, Goethe-University, Frankfurt, Germany; German Cancer Consortium (DKTK), Partner Site, Frankfurt, Germany; German Cancer Research Center (DKFZ), Heidelberg, Germany. Electronic address:

Interferons (IFNs) are key players in the tumor immune response and act by inducing the expression of IFN-stimulated genes (ISGs). Here, we identify the mixed-lineage kinase domain-like pseudokinase (MLKL) as an ISG in various cancer cell lines. Both type I and type II IFNs increase the expression of MLKL indicating that MLKL up-regulation is a general feature of IFN signaling. Read More

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December 2018
1 Read

An integrated quantitative proteomic and metabolomics approach to reveal the negative regulation mechanism of LamB in antibiotics resistance.

J Proteomics 2018 Dec 3. Epub 2018 Dec 3.

Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring (School of Life Sciences), Fujian Agriculture and Forestry University, Fuzhou 350002, PR China; Key Laboratory of Crop Ecology and Molecular Physiology (Fujian Agriculture and Forestry University), Fujian Province University, Fuzhou 350002, PR China. Electronic address:

Previously, a maltose-specific channel porin, LamB was found to be associate with multi-drug resistance in a lamB deleted strain, but the exact mechanisms require further elucidation. Herein, differential protein expression between the Escherichia coli mutant strain ΔlamB and the wild type strain BW25113, with and without ciprofloxacin (CFLX), was identified using iTRAQ based liquid chromatography-tandem mass spectrometry (LC-MS/MS); while differential metabolite expression was examined using gas chromatography-mass spectrometry (GC-MS). Further Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that lamB deletion leads to a decrease in several key metabolic pathways such as tricarboxylic acid (TCA) cycle, pentose phosphate pathway and glycolysis/gluconeogenesis. Read More

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December 2018

Non-GVHD ocular complications after hematopoietic cell transplantation: expert review from the Late Effects and Quality of Life Working Committee of the CIBMTR and Transplant Complications Working Party of the EBMT.

Biol Blood Marrow Transplant 2018 Dec 3. Epub 2018 Dec 3.

Department of Ophthalmology, Hospital Universitario Puerta de Hierro, Madrid, Spain.

Non-graft-versus-host disease (non-GVHD) ocular complications are generally uncommon after hematopoietic cell transplantation (HCT), but can cause prolonged morbidity affecting activities of daily living and quality of life. Here we provide an expert review of non-GVHD ocular complications in a collaboration between transplant physicians and ophthalmologists through the Late Effects and Quality of Life Working Committee of the Center for International Blood and Marrow Transplant Research and the Transplant Complications Working Party of the European Society of Blood and Marrow Transplantation. Complications discussed in this review include cataracts, glaucoma, ocular infections, ocular involvement with malignancy, ischemic microvascular retinopathy, central retinal vein occlusion, retinal hemorrhage, retinal detachment and ocular toxicities associated with medications. Read More

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December 2018
5 Reads

Loss of enzyme activity in mutated B4GALNT1 gene products in patients with hereditary spastic paraplegia results in relatively mild neurological disorders: Similarity with phenotypes of B4galnt1 knockout mice.

Neuroscience 2018 Dec 3. Epub 2018 Dec 3.

Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, Kasugai 487-8501, Japan; Department of Biochemistry II, Nagoya University Graduate School of Medicine, Nagoya 466-0065, Japan. Electronic address:

B4GALNT1 is an enzyme essential for the synthesis of complex gangliosides, whose absence leads to progressive neurodegeneration with aging in mice. Recently, eleven cases of hereditary spastic paraplegia with mutation in the coding region of B4GALNT1 were reported. However, changes in the enzymatic activity of their products have never been studied. Read More

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December 2018
2 Reads

EGFR-mutant SCLC exhibits heterogeneous phenotypes and resistance to common antineoplastic drugs.

J Thorac Oncol 2018 Dec 3. Epub 2018 Dec 3.

Department of Internal Medicine, National Taiwan University Hospital, Taiwan. Electronic address:

Introduction: Approximately 5% of patients with EGFR-activating mutations acquire EGFR-TKIs resistance through SCLC transformation. However, the reason for the poor outcome and the molecular basis of EGFR-mutant SCLC that has transformed from adenocarcinoma remain unclear.

Methods: In this study, we established 2 EGFR-mutant SCLC cell lines from lung adenocarcinoma patients after failed EGFR-TKI treatment to investigate their molecular basis and potential therapeutic strategies in the hope of improving patient outcome. Read More

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December 2018
4 Reads

Enrichment of common carp (Cyprinus carpio) fingerlings diet with Psidium guajava: The effects on cutaneous mucosal and serum immune parameters and immune related genes expression.

Fish Shellfish Immunol 2018 Dec 3. Epub 2018 Dec 3.

Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address:

The present study aimed at evaluation of possible effects of Psidium guajava as immunostimulant on mucosal and serum immunity, as well as related genes expression in common carp (Cyprinus carpio) fingerlings. In an eight weeks feeding trial, fish were fed with experimental diets containing guava leaf powder [GLP] (0, 0.25, 0. Read More

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December 2018
5 Reads

TroCCL4, a CC chemokine of Trachinotus ovatus, is involved in the antimicrobial immune response.

Fish Shellfish Immunol 2018 Dec 3. Epub 2018 Dec 3.

State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, PR China.

CC chemokines are a large subfamily of chemokines that play an important role in the innate immune system. To date, several CC chemokines have been identified in fish species; however, the activities and functions of these putative chemokines remain ambiguous in teleosts, especially in the golden pompano, Trachinotus ovatus. Here, we characterized CC chemokine ligand 4 from T. Read More

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December 2018
2 Reads

Ivabradine abrogates TNF-α-induced degradation of articular cartilage matrix.

Int Immunopharmacol 2018 Dec 3;66:347-353. Epub 2018 Dec 3.

Department of orthopedics, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, China. Electronic address:

Ivabradine is most commonly used for the treatment of worsening cardiac failure in patients who cannot tolerate the maximum dose of β-blockers or in whom treatment with β-blockers is contraindicated. While ivabradine is regarded as a highly selective "funny current" (I) inhibitor, the molecular mechanism behind the effect of this drug remains poorly understood. In the present study, we applied ivabradine in the context of osteoarthritis by treating primary human chondrocytes with tumor necrosis factor-α (TNF-α) and measuring degradation of the articular cartilage matrix as well as the expression of various enzymes and pro-inflammatory cytokines. Read More

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December 2018
5 Reads

Bipolar Disorder and 1513A>C P2RX7 polymorphism frequency.

Neurosci Lett 2018 Dec 3. Epub 2018 Dec 3.

Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, 90035-003 Porto Alegre, RS, Brazil. Electronic address:

Although the etiology of Bipolar Disorder (BD) remains unknown, a strong genetic component to the pathogenesis and risk for this disorder has been widely hypothesized. Several risk genes for BD have been identified; of these, the purinergic P2 × 7 receptor (P2 × 7R) constitutes a pro-inflammatory receptor and a potential risk gene candidate. The purpose of the present study was to assess the frequency of the 1513 A > C P2RX7 polymorphism (rs3751143; Glu496Ala), which leads to receptor loss-of-function, in 154 BD patients versus 184 control subjects. Read More

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December 2018
1 Read

Lipid-gene regulatory network reveals coregulations of triacylglycerol with phosphatidylinositol/lysophosphatidylinositol and with hexosyl-ceramide.

Biochim Biophys Acta Mol Cell Biol Lipids 2018 Dec 3. Epub 2018 Dec 3.

State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Lipid homeostasis is important for executing normal cellular functions and maintaining physiological conditions. The biophysical properties and intricate metabolic network of lipids underlie the coordinated regulation of different lipid species in lipid homeostasis. To reveal the homeostatic response among different lipids, we systematically knocked down 40 lipid metabolism genes in Drosophila S2 cells by RNAi and profiled the lipidomic changes. Read More

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December 2018
1 Read

Patatin-like lipolytic acyl hydrolases and galactolipid metabolism in marine diatoms of the genus Pseudo-nitzschia.

Biochim Biophys Acta Mol Cell Biol Lipids 2018 Dec 3. Epub 2018 Dec 3.

CNR-Istituto di Chimica Biomolecolare, Via Campi Flegrei 34, 80078, Pozzuoli, Naples, Italy. Electronic address:

Diatoms are eukaryotic microalgae that play a pivotal role in biological and geochemical marine cycles. These microorganisms are at the basis of the trophic chain and their lipids are essential components (e.g. Read More

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December 2018
1 Read

Geodorum densiflorum rhizome lectin inhibits Ehrlich ascites carcinoma cell growth by inducing apoptosis through the regulation of BAX, p53 and NF-κB genes expression.

Int J Biol Macromol 2018 Dec 4. Epub 2018 Dec 4.

Department of Biochemistry and Molecular Biology, Faculty of Science, University of Rajshahi, Rajshahi 6205, Bangladesh. Electronic address:

A lectin with a molecular mass of 12 ± 1 kDa was isolated for the first time from Geodorum densiflorum (Lam.) rhizome (GDL). The lectin exhibited hemagglutination activity both in mice and human erythrocytes which was inhibited by 4-nitrophenyl-β-D-glucopyranoside among the tested 26 sugars. Read More

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December 2018
2 Reads
2.858 Impact Factor

Study of the selenizing Codonopsis pilosula polysaccharides protects RAW264.7 cells from hydrogen peroxide-induced injury.

Int J Biol Macromol 2018 Dec 3. Epub 2018 Dec 3.

Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health in Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China. Electronic address:

Codonopsis pilosula polysaccharides (CPPS) has been shown to possess a variety of biological activities. In previous study, CPPS was successfully modified to obtain its best selenizing Codonopsis pilosula polysaccharides (sCPPS). The purpose of this study was to investigate the protective effect of the selenizing derivative of CPPS (sCPPS) from HO-induced oxidative damage in RAW264. Read More

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December 2018
3 Reads

Combined synergetic potential of metformin loaded pectin-chitosan biohybrids nanoparticle for NIDDM.

Int J Biol Macromol 2018 Dec 3. Epub 2018 Dec 3.

Department of Pharmaceutics, GITAM University, Rushikonda, Visakapatnam 530045, Andhra Pradesh, India.

Rationale: Combined therapy is a promising approach over its preference to minimize the dose, adverse effects and enhanced therapeutic efficiency in a various diseases including diabetes.

Aim: The present research work is to explore combined synergistic anti-diabetic potential of chitosan and pectin with Metformin (CPM) nano-formulation, with special emphasis on effect of Metformin when integrated with bio polymers.

Methods: The biohybrid nanoparticles (CPMNP) were formulated by ionic gelation process. Read More

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December 2018
1 Read

Draft genome analysis of lignocellulolytic enzymes producing Aspergillus terreus with structural insight of β-glucosidases through molecular docking approach.

Int J Biol Macromol 2018 Dec 3. Epub 2018 Dec 3.

Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388110, Gujarat, India.

Members of the genus Aspergillus are extensively studied ascomycetes because of their ability to synthesize high value-added compounds and enzymes of industrial interest. Precise whole genome assembly and gene annotation are significant for gene functional analyses. Here, we report the draft genome sequencing, assembly and whole genome analysis of Aspergillus terreus P14_T3, isolated from rumen sample of cattle fed with coconut-coir. Read More

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December 2018
1 Read

Identification and molecular analysis of a lncRNA-HOTAIR transcript from secondary hair follicle of cashmere goat reveal integrated regulatory network with the expression regulated potentially by its promoter methylation.

Gene 2018 Dec 3. Epub 2018 Dec 3.

College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR China. Electronic address:

The HOTAIR transcript is transcribed from the antisense strand within the HOXC gene cluster, and it is thought to play a role in regulating the inductive capacity of dermal papilla cells during the reconstruction of hair-follicle. In the current investigation, we firstly isolated and characterized a lncRNA-HOTAIR transcript from the secondary hair follicle of cashmere goat. Also, we analyzed its transcriptional pattern and methylation level of HOTAIR gene promoter in secondary hair follicle of cashmere goat during anagen and telogen stages. Read More

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December 2018
1 Read

Gene polymorphisms of angiotensin-converting enzyme and angiotensinogen and risk of idiopathic ischemic stroke.

Gene 2018 Dec 3. Epub 2018 Dec 3.

Unidad de Investigación Médica en Trombosis, Hemostasia y Aterogénesis, H.G.R. No 1. Dr. "Carlos Mac Gregor Sánchez Navarro" Instituto Mexicano del Seguro Social, Gabriel Mancera No. 222, Colonia Del Valle, CP 03100 Ciudad de México, Mexico.

Objective: The renin-angiotensin system (RAS) is a hormonal signaling mechanism implicated in the atherosclerosis and regulation of blood pressure. Angiotensin-converting enzyme (ACE) a key enzyme in the RAS, plays important roles in vascular remodeling atherosclerosis, and ischemic stroke. The aim of this study was to examine the possible contribution of the I/D in the ACE gene, M235T and T174M in the angiotensinogen (AGT) gene polymorphisms with ischemic stroke in young Mexican population. Read More

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December 2018
1 Read

HOXA5 overexpression promotes osteosarcoma cell apoptosis through the p53 and p38α MAPK pathway.

Gene 2018 Dec 3. Epub 2018 Dec 3.

Department of Traumatic Orthopedics, The Second Hospital Affiliated to Lanzhou University, Lanzhou City, Gansu Province 730000, China.

Osteosarcoma is the most common malignant bone tumor in children and adolescents. Aberrant expression of HOXA5 results in various diseases, including cancers. However, the specific function and molecular mechanism of HOXA5 in osteosarcoma is not fully understood. Read More

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December 2018
1 Read

Differentiation between persistent infection/colonization and re-infection/re-colonization of Mycobacterium abscessus isolated from patients in Northeast Thailand.

Infect Genet Evol 2018 Dec 3. Epub 2018 Dec 3.

Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand. Electronic address:

Mycobacterium abscessus can cause true infection or be present in the host as a harmless colonist. The ability of M. abscessus to cause disease and develop drug resistance is known to have a genetic basis. Read More

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December 2018
2 Reads

A network approach to quantifying radiotherapy effect on cancer: radiosensitive gene group centrality.

J Theor Biol 2018 Dec 3. Epub 2018 Dec 3.

School of Electrical, Computer and Energy Engineering, Arizona State University, Tempe, AZ 85287, USA.; Department of Physics, Arizona State University, Tempe, Arizona 85287, USA.

Radiotherapy plays a vital role in cancer treatment, for which accurate prognosis is important for guiding sequential treatment and improving the curative effect for patients. An issue of great significance in radiotherapy is to assess tumor radiosensitivity for devising the optimal treatment strategy. Previous studies focused on gene expression in cells closely associated with radiosensitivity, but factors such as the response of a cancer patient to irradiation and the patient survival time are largely ignored. Read More

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December 2018

MiR-15b is a key regulator of proliferation and apoptosis of chondrocytes from patients with condylar hyperplasia by targeting IGF1, IGF1R and BCL2.

Osteoarthritis Cartilage 2018 Dec 3. Epub 2018 Dec 3.

Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Wuhan University, Wuhan, Hubei province, China. Electronic address:

Objective: This study aimed to explore potential microRNAs (miRNAs), which participate in the pathological process of condylar hyperplasia (CH) through targeting specific proliferation- and apoptosis-related genes of chondrocytes.

Methods: Insulin-like growth factor 1 (IGF1), IGF1 receptor (IGF1R) and B-cell CLL/lymphoma 2 (BCL2) in CH cartilage were detected by real-time PCR, Western blot, immunohistochemistry and immunofluorescence. MiRanda and TargetScanS algorithms were used to predict certain miRNAs in CH chondrocytes concurrently modulating the above three genes. Read More

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December 2018
1 Read

Neuroevolution as a Tool for Microarray Gene Expression Pattern Identification in Cancer Research.

J Biomed Inform 2018 Dec 3. Epub 2018 Dec 3.

Institute of Informatics, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. Electronic address:

Microarrays are still one of the major techniques employed to study cancer biology. However, the identification of expression patterns from microarray datasets is still a significant challenge to overcome. In this work, a new approach using Neuroevolution, a machine learning field that combines neural networks and evolutionary computation, provides aid in this challenge by simultaneously classifying microarray data and selecting the subset of more relevant genes. Read More

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December 2018
2 Reads

Genetic inactivation of hypoxia inducible factor 1-alpha (HIF-1α) in adult hippocampal progenitors impairs neurogenesis and pattern discrimination learning.

Neurobiol Learn Mem 2018 Dec 3. Epub 2018 Dec 3.

Department of Neuroscience, University of New Mexico Health Sciences Center, Albuquerque, New Mexico. Electronic address:

HIF-1α is a hypoxia-inducible protein that regulates many cellular processes, including neural stem cell maintenance. Previous work demonstrated constitutive stabilization of HIF-1α in neural stem cells (NSCs) of the adult mouse subventricular zone (SVZ) and hippocampal subgranular zone (SGZ). Genetic inactivation of NSC-encoded HIF-1α in the adult SVZ results in gradual loss of NSCs, but whether HIF-1α is required for the maintenance of SGZ hippocampal progenitors and adult hippocampal neurogenesis has not been determined. Read More

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December 2018

5- lipoxygenase (ALOX5): genetic susceptibility to type 2 diabetes and vitamin D effects on monocytes.

J Steroid Biochem Mol Biol 2018 Dec 3. Epub 2018 Dec 3.

Department of Internal Medicine I, Division of Endocrinology, Diabetes and Metabolism, Goethe University Hospital, Frankfurt am Main, Germany.

The arachidonate 5-lipoxygenase (ALOX5) pathway has been implicated in chronic inflammatory disease which may be influenced by vitamin D due to vitamin D response elements (VDRE). We investigated an ALOX5 polymorphism (rs4987105) in patients with type 2 diabetes (T2D) and the in vitro effects of calcitriol (1,25(OH)D) on ALOX5 metabolism in monocytes of T2D patients and healthy controls (HC). 533 T2D and 473 HC were genotyped for the rs4987105 polymorphism. Read More

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December 2018

Engineered Viral RNA Decay Intermediates to Assess XRN1-mediated decay.

Methods 2018 Dec 3. Epub 2018 Dec 3.

Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80525, United States; Program in Cell & Molecular Biology, Colorado State University, Fort Collins, CO 80525, United States. Electronic address:

Both RNA synthesis and decay must be balanced within a cell to achieve proper gene expression. Additionally, modulation of RNA decay specifically offers the cell an opportunity to rapidly reshape the transcriptome in response to specific stimuli or cues. Therefore, it is critical to understand the underlying mechanisms through which RNA decay contribute to gene expression homeostasis. Read More

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December 2018
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Virulence properties of Vibrio vulnificus isolated from diseased zoea of freshness shrimp Macrobrachium rosenbergii.

Microb Pathog 2018 Dec 3. Epub 2018 Dec 3.

College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China. Electronic address:

Macrobrachium rosenbergii is one of the most economically important freshwater shimp, with fast growth and high nutrient content in the agricultural development of China. However, it had been suffering diseases infection, causing mass death and great economic losses. In the present study, a bacteria strain was isolated from the diseased zoea of M. Read More

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December 2018

Development and biochemical characterization of a mouse model of Parkinson's disease bearing defective glucocerebrosidase activity.

Neurobiol Dis 2018 Dec 3. Epub 2018 Dec 3.

Laboratory of Functional Neurochemistry, IRCCS Mondino Foundation, Pavia, Italy. Electronic address:

GBA1 gene encode for the lysosomal membrane protein glucocerebrosidase (GCase). GBA1 heterozygous mutations profoundly impair GCase activity and are currently recognized as an important risk factor for the development of Parkinson's disease (PD). Deficits in lysosomal degradation pathways may contribute to pathological α-synuclein accumulation, thereby favoring dopaminergic neuron degeneration and associated microglial activation. Read More

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December 2018

Mechanistic role of the CREB-regulated transcription coactivator 1 in cardiac hypertrophy.

J Mol Cell Cardiol 2018 Dec 3. Epub 2018 Dec 3.

Department of Clinical Pharmacology and Toxicology, Cardiovascular Research Center, University Medical Center Hamburg-Eppendorf, Martinistr. 52, 20246 Hamburg, Germany; DZHK (German Center for Cardiovascular Research), Partner Site Hamburg, Kiel, Lübeck, Germany; Institute of Pharmacy, University of Hamburg, Bundesstr. 45, 20146 Hamburg, Germany. Electronic address:

The sympathetic nervous system is the main stimulator of cardiac function. While acute activation of the β-adrenoceptors exerts positive inotropic and lusitropic effects by increasing cAMP and Ca, chronically enhanced sympathetic tone with changed β-adrenergic signaling leads to alterations of gene expression and remodeling. The CREB-regulated transcription coactivator 1 (CRTC1) is activated by cAMP and Ca. Read More

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December 2018
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Enzymes of glycerol-3-phosphate pathway in triacylglycerol synthesis in plants: Function, biotechnological application and evolution.

Prog Lipid Res 2018 Dec 3. Epub 2018 Dec 3.

Programa de Pós-Graduação em Genética e Biologia Molecular, Departmento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul (UFRGS), Brazil.. Electronic address:

Triacylglycerols (TAG) are the major form of energy storage in plants. TAG are primarily stored in seeds and fruits, but vegetative tissues also possess a high capacity for their synthesis and storage. These storage lipids are essential to plant development, being used in seedling growth during germination, pollen development, and sexual reproduction, for example. Read More

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December 2018
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