2,539,376 results match your criteria Gene Expression Patterns [Journal]


Behavioral, molecular and physiological responses of embryo-larval zebrafish exposed to types I and II pyrethroids.

Chemosphere 2018 Dec 6;219:526-537. Epub 2018 Dec 6.

Department of Environmental Toxicology, The Institute of Environmental and Human Health, Texas Tech University, Lubbock, TX 79416, USA.

Pyrethroids are potent neurotoxicants that may elicit multiple pathways of toxicity in non-target organisms. Comparative studies on the mechanistic and developmental effects of types I and II pyrethroids against non-target aquatic species are limited. This study assessed the effects of the two pyrethroid types against embryo-larval zebrafish (Danio rerio) at environmentally relevant and laboratory concentrations. Read More

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December 2018

Developmental toxicity of kresoxim-methyl during zebrafish (Danio rerio) larval development.

Chemosphere 2018 Dec 8;219:517-525. Epub 2018 Dec 8.

Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Hangzhou, 310021, Zhejiang, China. Electronic address:

Kresoxim-methyl (KM) is a broad spectrum strobilurin fungicide that has been used widely on crops around the world. In the present study, we aimed to investigate the toxic effects of KM using various sublethal endpoints during zebrafish (Danio rerio) larval development. Results showed that the LC values of KM to zebrafish at multiple life stages (embryo, larvae, juvenile and adult) were 0. Read More

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December 2018
1 Read

Proteomic analysis for phenanthrene-elicited wheat chloroplast deformation.

Environ Int 2018 Dec 11;123:273-281. Epub 2018 Dec 11.

Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA 01003, USA.

The exposure of polycyclic aromatic hydrocarbons (PAHs) can cause wheat leaf chlorosis. Thus, we hypothesize that chloroplast inner structure damage is the reason for leaf chlorosis. This study was conducted with the wheat seedlings exposed to Hoagland nutrient solution containing 1. Read More

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December 2018
4 Reads

An update on Drug Repurposing: Re-written saga of the drug's fate.

Biomed Pharmacother 2018 Dec 12;110:700-716. Epub 2018 Dec 12.

Pharamacological Modelling and Simulation Centre, M.S.Ramaiah University of Applied Sciences, Bangalore, Karnataka, India; Department of Pharmacy Practice, Faculty of Pharmacy, M.S.Ramaiah University of Applied Sciences, Bangalore, Karnataka, India.

Drug repurposing is an unconventional drug discovery approach to explore new therapeutic benefits of existing, shelved and the drugs in clinical trials. This approach is currently emerging to overcome the bottleneck constraints faced during traditional drug discovery in grounds of financial support, timeline and resources. In this direction, several efforts were made for the construction of stratagems based on bioinformatics and computational tools to intensify the repurposing process off-late. Read More

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December 2018
1 Read

Clinicopathological characteristics of metaplastic breast cancer - analysis of the basic immunohistochemical profile and comparison with other invasive breast cancer types.

Breast 2018 Dec 7;43:135-141. Epub 2018 Dec 7.

Department of Biophysics and Human Physiology, Medical University of Warsaw, Chalubinskiego 5, 02-004 Warsaw, Poland.

Introduction: Metaplastic breast cancer (MpBC) is a rare but aggressive type of breast cancer accounting for 0.25-1% of all diagnosed invasive breast cancers. Morphologically, it is characterized by differentiation of the neoplastic epithelium into squamous cells and/or mesenchymal-looking tissue. Read More

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December 2018

Mesenchymal stromal cells isolated from human fetal liver release soluble factors with a potential role in liver tissue repair.

Differentiation 2018 Dec 5;105:14-26. Epub 2018 Dec 5.

Department of Research, IRCCS-ISMETT, Palermo, Italy.

We isolated a population of proliferating cells from cultured human fetal hepatocytes of 16-22 weeks gestational age. The cells shared a similar phenotype to that of mesenchymal stromal cells (MSCs) according to the International Society for Cellular Therapy (ISCT), including plastic adherence, antigen expression profile, and in vitro multilineage differentiation potential. Fetal liver (FL)-MSCs expressed the albumin gene, and harbored a subpopulation of CK18 cells (20-40%), which defined their hepatic origin. Read More

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December 2018
1 Read

Housefly larvae (Musca domestica) significantly accelerates degradation of monensin by altering the structure and abundance of the associated bacterial community.

Ecotoxicol Environ Saf 2018 Dec 12;170:418-426. Epub 2018 Dec 12.

College of Veterinary Medicine, China Agricultural University, No. 2 Yuan Ming Yuan West Road, Hai Dian District, Beijing 100193, China. Electronic address:

Vermicomposting of livestock manure using housefly larvae is a promising biotechnology for waste reduction and control of antibiotic pollution. Monensin (MON), an ionophore polyether antibiotic (IPA), is widely used in broiler feed to control coccidiosis. However, MON residues in litter have become a major source of pollution in the environment. Read More

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December 2018
2 Reads

Excess copper promotes photoinhibition and modulates the expression of antioxidant-related genes in Zostera muelleri.

Aquat Toxicol 2018 Dec 5;207:91-100. Epub 2018 Dec 5.

Climate Change Cluster, University of Technology Sydney, NSW, Australia.

Copper (Cu) is an essential micronutrient for plants and as such is vital to many metabolic processes. Nevertheless, when present at elevated concentrations, Cu can exert toxic effects on plants by disrupting protein functions and promoting oxidative stress. Due to their proximity to the urbanised estuaries, seagrasses are vulnerable to chemical contamination via industrial runoff, waste discharges and leachates. Read More

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December 2018

Polyphenolic profiling of roots (Vitis spp.) under grape phylloxera (D. vitifoliae Fitch) attack.

Plant Physiol Biochem 2018 Dec 4;135:174-181. Epub 2018 Dec 4.

Division of Viticulture and Pomology, Department of Crop Sciences, University of Natural Resources and Life Sciences (BOKU), Vienna, Konrad Lorenz Str. 24, 3430, Tulln, Austria.

Many plants respond to herbivore attacks by the formation of secondary metabolites, such as polyphenols. Grape phylloxera (Daktulosphaira vitifoliaeFitch) induces organoid root galls on fibrous root tips of tolerant Vitis spp. rootstocks. Read More

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December 2018
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Cladophora glomerata methanolic extract decreases oxidative stress and improves viability and mitochondrial potential in equine adipose derived mesenchymal stem cells (ASCs).

Biomed Pharmacother 2018 Dec 12;111:6-18. Epub 2018 Dec 12.

Department of Experimental Biology, Faculty of Biology and Animal Science, Wrocław University of Environmental and Life Sciences, Norwida 27B, 50-375 Wrocław, Poland; Faculty of Veterinary Medicine, Equine Clinic - Equine Surgery, Justus-Liebig-University, 35392 Gießen, Germany. Electronic address:

Reactive oxygen species (ROS) are key mediators of several cellular damage and thus associated with equine diseases such as inflammation and metabolic syndrome. This study aimed to evaluate the protective and antioxidant activities of methanolic extract prepared from Cladophora glomerata (C. glomerata) biomass, on equine adipose derived mesenchymal stem cells (EqASCs), under experimental oxidative stress induced by HO. Read More

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December 2018

Successful genetic modification of porcine spermatogonial stem cells via an electrically responsive Au nanowire injector.

Biomaterials 2018 Dec 10;193:22-29. Epub 2018 Dec 10.

Department of Animal Life Science, Kangwon National University, Chuncheon, 24341, South Korea; Department of Applied Animal Science, Kangwon National University, Chuncheon, 24341, South Korea. Electronic address:

Transgenic pigs are quite useful in many biomedical fields, such as xenotransplantation research and the production of biopharmaceutical materials. The genetic transformation of porcine spermatogonial stem cells (pSSCs) followed by differentiation into mature spermatozoa enables the effective production of transgenic pigs. Improving the transfection efficiency of pSSCs, however, has been much desired. Read More

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December 2018

Phylogenomics of Thecamoebida (Discosea, Amoebozoa) with the Description of Stratorugosa tubuloviscum gen. nov. sp. nov., a Freshwater Amoeba with a Perinuclear MTOC.

Protist 2018 Oct 16;170(1):8-20. Epub 2018 Oct 16.

Spelman College, 350 Spelman Lane Southwest, Atlanta, GA 30314, USA.

Thecamoebida Smirnov and Cavalier-Smith, 2011 (Discosea, Amoebozoa) has been molecularly understudied. The group until recently consisted of three genera containing species that live in terrestrial or aquatic environments. Here, we describe a fourth genus, Stratorugosa tubuloviscum gen. Read More

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October 2018

Changes in the Rates and Population Structure of MRSA from bloodstream infections. A Single Center Experience (2000-2015).

J Glob Antimicrob Resist 2018 Dec 12. Epub 2018 Dec 12.

First Department of Medicine, "Laikon" General Hospital, National and Kapodistrian University of Athens, 11527, Athens, Greece. Electronic address:

Objectives: We aimed to assess the MRSA Bloodstream Infection (BSI) rates and the population structure of the MRSA isolates recovered between 2000 and 2015 in a tertiary care hospital in Athens, Greece.

Methods: Non-repetitive MRSA blood isolates recovered during the study period were examined. Antimicrobial susceptibility testing was performed by Kirby-Bauer and the gradient strip methods Carriage of the PVL and mecA genes were examined by PCR. Read More

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December 2018

Molecular Study of Resistance of Staphylococcus aureus to Antiseptic Quaternary Ammonium Sulfate.

J Glob Antimicrob Resist 2018 Dec 12. Epub 2018 Dec 12.

Clinical Pathology, Helwan Faculty of Medicine, Egypt.

Objective: The goals of the present study were to i.determine the prevalence of qac and smr genes in clinical isolates of S.aureus from hospital acquired infections ii. Read More

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December 2018

Draft genome sequence of human-associated streptogramin resistant Staphylococcus aureus.

J Glob Antimicrob Resist 2018 Dec 12. Epub 2018 Dec 12.

Bacterial Epidemiology and Antimicrobial Resistance Research Unit, USDA-ARS, Athens, Georgia, USA. Electronic address:

Objectives: Staphylococcus aureus is one of the leading causes of nosocomial and community-acquired infections. Treatment of those infections with macrolide-lincosamide-streptogramin (MLS) antibiotics has led to resistance to those antibiotics through various mechanisms. S. Read More

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December 2018
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Draft genome sequence of Escherichia coli ST410 co-harboring bla, bla, bla, aac(3)-IIa, aac(6')-Ib-cr genes with gyrA and parC mutations isolated from pediatric patient in Poland.

J Glob Antimicrob Resist 2018 Dec 12. Epub 2018 Dec 12.

Department of Bacteriology and Biocontamination Control, National Institute of Public Health-National Institute of Hygiene, Warsaw, Poland.

Objectives: Escherichia coli is one of the major agents of nosocomial infections. The aim of this study was to report the first draft genome sequence of an E. coli strain (no. Read More

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December 2018
2 Reads

Bubble-free rapid microfluidic PCR.

Biosens Bioelectron 2018 Oct 11;126:725-733. Epub 2018 Oct 11.

Department of Bioengineering, University of California Berkeley, CA 94720, USA; Berkeley Sensor and Actuator Center, University of California Berkeley, CA 94720, USA; UC Berkeley and UCSF Joint Graduate Program in Bioengineering, Berkeley/San Francisco, USA; Department of Electrical Engineering and Computer Science, University of California Berkeley, CA 94720, USA; Biophysics Graduate Program, University of California Berkeley, CA 94720, USA. Electronic address:

Microfluidic polymerase chain reaction (PCR) has been of great interest owing to its ability to perform rapid and specific nucleic acid amplification and analysis on small volumes of samples. One of the major drawbacks of microfluidic PCR is bubble generation and reagent evaporation, which can cause malfunctions. Here, through theoretical modeling and characterization of bubble behavior, we propose a bubble-free microfluidic PCR device via controlled fluid transfer. Read More

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October 2018
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Key role of cyromazine in the distribution of antibiotic resistance genes and bacterial community variation in aerobic composting.

Bioresour Technol 2018 Dec 3;274:418-424. Epub 2018 Dec 3.

College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.

The risks that have not been noted so far have come from the use of non-antibiotics. In this study, non-antibiotic drug (cyromazine) was used in composting to investigate its possible effects on the distribution of ARGs and changes of bacterial community. Results showed that cyromazine increased the abundances of highly-risky ARGs (bla and bla), and heavy metal resistance genes (MRGs). Read More

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December 2018
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Characterization of vanM carrying clinical Enterococcus isolates and diversity of the suppressed vanM gene cluster.

Infect Genet Evol 2018 Dec 12. Epub 2018 Dec 12.

Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China; Key Laboratory of Microorganism Technology and Bioinformatics Research of Zhejiang Province, Hangzhou, Zhejiang Province, China. Electronic address:

Here we report the prevalence of the suppressed vanM gene cluster as a reservoir of vancomycin resistance genes. Among 1284 clinical isolates of enterococci from four hospitals in Hangzhou, China, 55 isolates of Enterococcus faecium and one isolate of Enterococcus faecalis screened positive for the vanM genotype. Antimicrobial susceptibility testing showed that 55 of the 56 vanM-positive isolates were susceptible to vancomycin and teicoplanin. Read More

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December 2018

Ergosterol reduction impairs mitochondrial DNA maintenance in S. cerevisiae.

Biochim Biophys Acta Mol Cell Biol Lipids 2018 Dec 12. Epub 2018 Dec 12.

Department of Biology and Biotechnology "Charles Darwin", La Sapienza University of Rome, Piazzale A. Moro 5, 00185 Rome, Italy.; Pasteur Institute-Cenci Bolognetti Foundation, Viale Regina Elena, 291, 00100 Rome, Italy.. Electronic address:

Sterols are essential lipids, involved in many biological processes. In Saccharomyces cerevisiae, the enzymes of the ergosterol biosynthetic pathway (Erg proteins) are localized in different cellular compartments. With the aim of studying organelle interactions, we discovered that Erg27p resides mainly in Lipid Droplets (LDs) in respiratory competent cells, while in absence of respiration, is found mostly in the ER. Read More

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December 2018

High-fat diet induces aberrant hepatic lipid secretion in blunt snout bream by activating endoplasmic reticulum stress-associated IRE1/XBP1 pathway.

Biochim Biophys Acta Mol Cell Biol Lipids 2018 Dec 12. Epub 2018 Dec 12.

Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, People's Republic of China. Electronic address:

This study was conducted to understand the effect of high-fat diet challenge on lipid transport and endoplasmic reticulum stress in blunt snout bream. Ninety fish (average weight: 41.84 ± 0. Read More

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December 2018
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Epidemiology and etiology of diarrhea in UK military personnel serving on the United Nations Mission in South Sudan in 2017: A prospective cohort study.

Travel Med Infect Dis 2018 Dec 12. Epub 2018 Dec 12.

Academic Department of Military Medicine, Medical Directorate, Defence Medical Services, Birmingham, UK.

Background: . Diarrhea is a well-established problem in travellers, with military personnel at especially high risk. This study aimed to characterise the spectrum of pathogens causing diarrhea in UK military personnel in South Sudan, and assess the utility of culture-independent testing for etiology and antimicrobial resistance in a logistically challenging and austere environment. Read More

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December 2018

Glucocorticoid receptor (NR3C1) gene polymorphisms are associated with age and blood parameters in Polish Caucasian nonagenarians and centenarians.

Exp Gerontol 2018 Dec 12. Epub 2018 Dec 12.

Mossakowski Medical Research Centre, PAS, Pawinskiego 5, 02-106 Warsaw, Poland; Medical Centre of Postgraduate Education, Marymoncka 99/103, 01-813 Warsaw, Poland. Electronic address:

Introduction: Polymorphism of the glucocorticoid receptor gene (NR3C1) may modify protein abundance or function and therefore disturb human homeostasis.

Methods: Variant frequencies of the three NR3C1 polymorphisms, rs2963154, rs10515522 and rs2918418, selected in silico as associated with longevity, was analyzed in 552 DNA samples from 95 to 106-year-old individuals and in 284 samples of cord blood DNA from newborns.

Results: Frequencies of the TT genotypes of rs2963154 and rs10515522, and of the rs291841 CC genotype, were higher in the long-lived study subjects (p = 0. Read More

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December 2018

RelA driven co-expression of CXCL13 and CXCR5 is governed by a multifaceted transcriptional program regulating breast cancer progression.

Biochim Biophys Acta Mol Basis Dis 2018 Dec 12. Epub 2018 Dec 12.

Immunology Laboratory, Department of Zoology, University of Calcutta, Kolkata 700019, India. Electronic address:

Lethal metastasis of primary breast tumors to lymph nodes has been found to be associated with the co-expression of chemokine CXCL13 and its receptor CXCR5. To date, however, the precise molecular events regulating the co-expression of CXCL13 and CXCR5 in the context of breast cancer progression have not been identified. Therefore, to extend our understanding of the drivers of breast cancer metastasis, we undertook a line of investigation in this study in which we demonstrate that the transcriptional regulation of CXCL13 is mediated by the reciprocal activity of RelA and Nrf2, while CXCR5 is transcriptionally silenced by CpG island methylation within its promoter. Read More

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December 2018

The role of the wzy gene in lipopolysaccharide biosynthesis and pathogenesis of avian pathogenic Escherichia coli.

Microb Pathog 2018 Dec 12. Epub 2018 Dec 12.

Shanghai Veterinary Research Institute, The Chinese Academy of Agricultural Sciences (CAAS), 518 Ziyue Road, Shanghai, 200241, PR China. Electronic address:

Avian pathogenic Escherichia coli (APEC) causes severe respiratory and systemic diseases in poultry. The wzy gene encodes the O-antigen polymerase (Wzy), which plays an important role in the synthesis of the lipopolysaccharide (LPS) of bacteria. However, the function of the wzy gene in APEC remains unclear. Read More

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December 2018

Defective repair capacity of variant proteins of the DNA glycosylase NTHL1 for 5-hydroxyuracil, an oxidation product of cytosine.

Free Radic Biol Med 2018 Dec 12. Epub 2018 Dec 12.

Department of Tumor Pathology, Hamamatsu University School of Medicine, Hamamatsu, Japan.

The NTHL1 gene encodes DNA glycosylase, which is involved in base excision repair, and biallelic mutations of this gene result in NTHL1-associated polyposis (NAP), a hereditary disease characterized by colorectal polyposis and multiple types of carcinomas. However, no proper functional characterization of variant NTHL1 proteins has been done so far. Herein, we report functional evaluation of variant NTHL1 proteins to aid in the accurate diagnosis of NAP. Read More

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December 2018

Effects of cellular differentiation in human primary bronchial epithelial cells: Metabolism of 4-(methylnitrosamine)-1-(3-pyridyl)-1-butanone.

Toxicol In Vitro 2018 Dec 12. Epub 2018 Dec 12.

Division of Genetic and Molecular Toxicology, Division of Biochemical Toxicology, National Center for Toxicological Research, U.S Food and Drug Administration, Jefferson, AR 72079, United States. Electronic address:

Many of the toxicants in tobacco smoke undergo biotransformation in the lungs of smokers, both to reactive and to detoxified derivatives. Human air-liquid-interface (ALI) airway tissue models have emerged as an advanced in vitro model for evaluating the toxicity of inhaled substances; however, the metabolic potential of these cultures has not been evaluated extensively. In this study, we compared the metabolic activities of an ALI tissue model to the undifferentiated normal human primary bronchial epithelial (NHBE) cells from which it was derived. Read More

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December 2018
4 Reads

Anthriscus sylvestris root extract reduces allergic lung inflammation by regulating interferon regulatory factor 4-mediated Th2 cell activation.

J Ethnopharmacol 2018 Dec 12. Epub 2018 Dec 12.

Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon, 34054, Korea; Department of Pathology, College of Korean Medicine, Sangji University, Wonju-si, Gangwon-do, 26339, Korea. Electronic address:

Ethnopharmacological Relevance: Anthriscus sylvestris L. Hoffmann (AS) is a perennial plant that grows in Asia and Eastern Europe. Its dried root is used to treat conditions such as asthma, bronchitis, and cough. Read More

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December 2018
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The RNA helicase DHX33 is required for cancer cell proliferation in human glioblastoma and confers resistance to PI3K/mTOR inhibition.

Cell Signal 2018 Dec 12. Epub 2018 Dec 12.

Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong, China. Electronic address:

Human Glioblastoma is one deadly disease; the median survival time is reported to be 13.9 months after treatment. In the present study, we discovered that DHX33 is highly expressed in 84% of all Glioblastoma multiforme (GBM). Read More

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December 2018
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A potential regulatory network among WDR86-AS1, miR-10b-3p, and LITAF is possibly involved in preeclampsia pathogenesis.

Cell Signal 2018 Dec 12. Epub 2018 Dec 12.

Department of Obstetrics and Gynecology, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, PR China.

Preeclampsia (PE), a pregnancy-specific disorder, is a leading cause of perinatal maternal and fetal mortality and morbidity. Impaired migration and invasion of trophoblastic cells and an imbalanced systemic maternal inflammatory response have been proposed as possible causes of pathogenesis of PE. Comparative analysis of PE-affected placentas and healthy placentas has uncovered differentially expressed long noncoding RNAs, microRNAs, and mRNAs. Read More

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December 2018

Identification of a novel mutation in FBN1 in a Chinese family with inherited ectopia lentis by targeted NGS.

Gene 2018 Dec 12. Epub 2018 Dec 12.

Aier School of Ophthalmology, Central South University, Tianxin District, Changsha, Hunan Province 410015, China; WuhanAier Eye Hospital, Aier Eye Hospital Group, Wuchuan District, Wuhan, Hubei Province 430063, China. Electronic address:

Aims: To diagnose a Chinese family with inherited ectopia lentis in a genetic method and analyze the genotype-phenotype correlation.

Methods: The phenotype of each family member was identified by detailed clinical examination. We used targeted next generation sequencing (NGS) to identify mutations in FBN1 in an efficient and accurate way. Read More

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December 2018

FH535 inhibits proliferation and migration of colorectal cancer cells by regulating CyclinA2 and Claudin1 gene expression.

Gene 2018 Dec 12. Epub 2018 Dec 12.

Wenzhou Medical University School of Laboratory Medicine and Life Sciences, Wenzhou 325000, China. Electronic address:

Wnt signaling pathway plays a major role in the progression of colorectal cancer (CRC). Small molecules which can cut off this signal transduction can be promising anti-cancer drugs for CRC therapy. Therefore, we aimed to investigate the mechanisms of FH535, an inhibitor of the Wnt signaling pathway, on inhibiting proliferation and migration of colorectal cancer cells DLD-1 and SW620. Read More

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December 2018
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The clinicopathologic impacts and prognostic significance of GLUT1 expression in patients with lung cancer: A meta-analysis.

Gene 2018 Dec 12. Epub 2018 Dec 12.

Department of Thoracic Oncology, First Affiliated Hospital of Xi'an Medical College, Xi'an 710032, Shaanxi Province, PR China. Electronic address:

Background: Accumulating studies have reported that GLUT1 is aberrantly expressed in lung cancer; nevertheless, the clinicopathologic significance and the prognostic role of GLUT1 still remain controversial. The aim of this meta-analysis was to identify the clinicopathologic and prognostic implications of the GLUT1 expression in lung cancer patients.

Materials And Methods: Databases with literature published in English, including Cochrane Library, Embase, Web of Science, and PubMed, and the China National Knowledge Infrastructure (CNKI) and WanFang database in Chinese were searched comprehensively for relevant studies in August 2017. Read More

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December 2018

Genome-wide identification, characterization and expression analysis of the MITF gene in Yesso scallops (Patinopecten yessoensis) with different shell colors.

Gene 2018 Dec 12. Epub 2018 Dec 12.

Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, China. Electronic address:

The microphthalmia-associated transcription factor (MITF) is the center of the regulator network of melanin synthesis in vertebrates. However, the role of MITF in shell color formation is poorly studied in mollusks. In the present study, an MITF gene, PyMITF, was first identified at the whole-genome level in Yesso scallop (Patinopecten yessoensis), an evolutionarily and economically important species, the shell color of which shows polymorphism. Read More

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December 2018

Integrational analysis of miRNAs data sets as a plausible missing linker between Epstein-Barr virus and vitamin D in relapsing remitting MS patients.

Gene 2018 Dec 12. Epub 2018 Dec 12.

Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Electronic address:

Given the multifactorial state of autoimmune complex diseases such as multiple sclerosis (MS), it is not clear if different risk factors act jointly or independently. Despite intensive studies investigating multi aspects of MS risk factors, findings with regards to potential biomarkers that may link these risk factors remained largely inconclusive. System biology or data integration utilizes different validated datasets to extract meaningful information and map the plausible biological pathways and networks. Read More

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December 2018

Characterization of HPV integration, viral gene expression and E6E7 alternative transcripts by RNA-Seq: A descriptive study in invasive cervical cancer.

Genomics 2018 Dec 12. Epub 2018 Dec 12.

Genetics Program, Instituto Nacional de Câncer (INCA), Rio de Janeiro, Brazil. Electronic address:

Scarce data are available on the expression of Papillomavirus genome and the frequency of alternatively spliced E6/E7 mRNAs in invasive cervical cancer. We carried out a comprehensive characterization of HPV expression by RNA-Seq analysis in 22 invasive cervical cancer with HPV16 or HPV18, characterizing the presence of integrated/episomal viral DNA, the integration sites in human genome and the proportion of alternative splicing products of E6 and E7 genes. The expression patterns suggested the presence of episomal and/or integrated viral DNA, with integration detected in most tumors, frequently occurring within human genes in HPV18+ and in intergenic regions in HPV16+ tumors. Read More

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December 2018

Assembling a genome for novel nitrogen-fixing bacteria with capabilities for utilization of aromatic hydrocarbons.

Genomics 2018 Dec 12. Epub 2018 Dec 12.

CSIR-National Environmental Engineering Research Institute, Nehru Marg, Nagpur 440020, India. Electronic address:

Metagenome from refinery wastewater treatment plant running under nitrogen stress was analyzed for mining of novel aromatic hydrocarbon-degrading bacteria. The sequence data were assembled using metaspade followed by binning using the Metabat tool to assemble genome; where coverage and depth were calculated using bowtie and samtools. The analysis picked a novel genome belonging to family Bradyrhizobiaceae, identified based on 16S rDNA gene which was supported by CheckM and Kraken analysis. Read More

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December 2018

Critical role of Ca3.2 T-type calcium channels in the peripheral neuropathy induced by bortezomib, a proteasome-inhibiting chemotherapeutic agent, in mice.

Toxicology 2018 Dec 12. Epub 2018 Dec 12.

Laboratory of Pharmacology and Pathophysiology, Faculty of Pharmacy, Kindai University (formerly known as Kinki University), Higashi-Osaka, 577-8802, Japan. Electronic address:

Bortezomib, a first-line agent for treatment of multiple myeloma, exhibits anticancer activity through proteasome inhibition. However, bortezomib-induced peripheral neuropathy (BIPN) is one of the most serious side effects. Since decreased proteasomal degradation of Ca3. Read More

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December 2018

Alternative splicing of the vitamin D receptor modulates target gene expression and promotes ligand-independent functions.

Toxicol Appl Pharmacol 2018 Dec 12. Epub 2018 Dec 12.

Department of Environmental and Molecular Toxicology, Oregon State University, 1007 Agriculture & Life Sciences Building, Corvallis, OR 97331; USA; LS Pharma, 884 Park St., Lebanon, OR 97355; USA.

Alternative splicing modulates gene function by creating splice variants with alternate functions or non-coding RNA activity. Naturally occurring variants of nuclear receptor (NR) genes with dominant negative or gain-of-function phenotypes have been documented, but their cellular roles, regulation, and responsiveness to environmental stress or disease remain unevaluated. Informed by observations that class I androgen and estrogen receptor variants display ligand-independent signaling in human cancer tissues, we questioned whether the function of class II NRs, like the vitamin D receptor (VDR), would also respond to alternative splicing regulation. Read More

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December 2018

Localization of Contextual and Context Removed Auditory Fear Memory within the Basolateral Amygdala Complex.

Neuroscience 2018 Dec 12. Epub 2018 Dec 12.

School of Psychology and Counselling, Queensland University of Technology, Brisbane, Australia; Institute of Health and Biomedical Innovation, Queensland University of Technology at Translational Research Institute, Brisbane, Australia; Center for the Study of Traumatic Stress, Department of Psychiatry, USU School of Medicine, Bethesda, MD, USA. Electronic address:

Debilitating and persistent fear memories can rapidly form in humans following exposure to traumatic events. Fear memories can also be generated and studied in animals via Pavlovian fear conditioning. The current study was designed to evaluate basolateral amygdala complex involvement following the formation of different fear memories (two contextual fear memories and one adjusted auditory fear memory). Read More

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December 2018

Morus alba L. Diminishes visceral adiposity, insulin resistance, behavioral alterations via regulation of gene expression of leptin, resistin and adiponectin in rats fed a high-cholesterol diet.

Physiol Behav 2018 Dec 12. Epub 2018 Dec 12.

Department of Zoology, Faculty of Science, Ain Shams University, Cairo, Egypt. Electronic address:

Ethanolic extract of leaves of Morus alba L. (M. alba), known as white mulberry, was orally administered (100 mg/kg b. Read More

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December 2018

Administration of eicosapentaenoic and docosahexaenoic acids may improve the remodeling and browning in subcutaneous white adipose tissue and thermogenic markers in brown adipose tissue in mice.

Mol Cell Endocrinol 2018 Dec 12. Epub 2018 Dec 12.

Laboratory of Morphometry, Metabolism and Cardiovascular Diseases, Biomedical Center, Institute of Biology, State University of Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address:

The role of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in browning and thermogenesis has not been fully elucidated. Thus, we meant to evaluate the effect of EPA and DHA, administered alone or combined, with the activation of browning markers in subcutaneous white adipose tissue (sWAT), and thermogenic markers in brown adipose tissue (BAT). C57BL/6 adult male mice received a control diet or a high-fructose diet (HFru) for eight weeks, but after the first three weeks, HFru was divided into new groups: HFru, HFru + EPA, HFru + DHA, and HFru-EPA + DHA. Read More

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December 2018

Bronchiolitis obliterans after allogeneic hematopoietic stem cell transplantation: the effect of NOD2/CARD15 mutations in a Tunisian population.

Hum Immunol 2018 Dec 12. Epub 2018 Dec 12.

Immunogenetic Applied to Cells Therapy Research Unit, Immuno-Haematology and HLA-Typing Department, National Blood Transfusion Centre of Tunis, Tunis, Tunisia.

Bronchiolitis obliterans (BO) is a serious lung complication that can develop after allogenic stem cell transplantation. It has been suggested that single nucleotide polymorphisms (SNPs) that affect the NOD2/CARD15 gene impair its function and result in an uncontrolled innate immune response in the recipient, thereby leading to BO. One hundred eighty-one donor-recipient pairs were analyzed for the association between NOD2 gene variants (SNP8 [Arg702Trp], SNP12 [Gly908Arg], and SNP13 [Leu1007fsinsC]) and the occurrence of BO. Read More

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December 2018

The influence of the OPRM1 (A118G) polymorphism on behavioral and neural correlates of aggression in healthy males.

Neuropharmacology 2018 Dec 12. Epub 2018 Dec 12.

Department of Psychiatry, Psychotherapy and Psychosomatics, Faculty of Medicine, RWTH Aachen University, 52074, Aachen, Germany; JARA Institute Brain Structure Function Relationship Institute for Neuroscience and Medicine (INM 10), Forschungszentrum Jülich, 52425 Jülich, Germany.

Current models of aggression suggest that in addition to personality traits and environmental factors, biological vulnerability associated with genetics substantially impacts aggressive behavior. In a functional imaging study, we investigated the influence of the single nucleotide polymorphism of the mu 1 subtype opioid receptor gene (OPRM1), implicated in sociability, on correlates of trait and state aggression to delineate the function of these influences in aggression. A key aim was further to differentiate different aspects of aggressive reactions - namely, the reaction to provocation and the decision to punish an opponent. Read More

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December 2018

Identification of a circulating miRNA signature in extracellular vesicles collected from amyotrophic lateral sclerosis patients.

Brain Res 2018 Dec 12. Epub 2018 Dec 12.

Department of Chemistry and Biochemistry, Université de Moncton, 18 Antonine-Maillet Avenue, Moncton, New Brunswick, E1A 3E9, Canada. Electronic address:

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder associated with the progressive death of motor neurons. Mean survival for a patient diagnosed with ALS is between 2 to 5 years. Early and efficient diagnosis of the various forms of ALS remains a significant challenge, resulting in a need to identify clinically-relevant biomarkers in readily accessible body fluids. Read More

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December 2018

Cardio-omentopexy Reduces Cardiac Fibrosis and Heart Failure after Experimental Pressure Overload.

Ann Thorac Surg 2018 Dec 12. Epub 2018 Dec 12.

Department of Surgery, Northwestern University Feinberg School of Medicine, Chicago, IL; Division of Cardiovascular-Thoracic Surgery, Ann & Robert H. Lurie Children's Hospital of Chicago, Chicago, IL. Electronic address:

Background: The pedicled greater omentum has been shown to offer benefit in ischemic heart disease for both animal models and human patients. The impact of cardio-omentopexy in a pressure overload model of left ventricular hypertrophy (LVH) is unknown.

Methods: LVH was created in rats by banding the ascending aorta after right thoracotomy (n=23). Read More

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December 2018

Inhibition of SIRT1 by microRNA-9, the key point in process of LPS-induced severe inflammation.

Arch Biochem Biophys 2018 Dec 12. Epub 2018 Dec 12.

Department of Burns and Cutaneous Surgery, Xijing Hospital, The Fourth Military Medical University, 127 Changle West Road, Xi'an, Shaanxi, 710032, China. Electronic address:

Severe inflammation may lead to multiple organs dysfunction syndrome, which has a high mortality. MicroRNA is found participated in this process. In this study we developed a lipopolysaccharide-induced inflammation cell model on macrophages and a lipopolysaccharide-induced inflammation mouse model. Read More

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December 2018
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Darpp-32 and t-Darpp protein products of PPP1R1B: old dogs with new tricks.

Biochem Pharmacol 2018 Dec 12. Epub 2018 Dec 12.

Department of Chemistry and Biochemistry, California State University, Los Angeles, CA, USA. Electronic address:

The PPP1R1B gene is located on chromosome 17q12 (39,626,208-39,636,626[GRCh38/hg38]), which codes for multiple transcripts and two experimentally-documented proteins Darpp-32 and t-Darpp. Darpp-32 (Dopamine and cAMP Regulated Phosphoprotein), discovered in the early 1980s, is a protein whose phosphorylation is upregulated in response to cAMP in dopamine-responsive tissues in the brain. It's phosphorylation profile modulates its ability to bind and inhibit Protein Phosphatase 1 activity, which, in turn, controls the activity of hundreds of phosphorylated proteins. Read More

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December 2018
2 Reads

Myosin Heavy Chain 10 (MYH10) Gene Silencing Reduces Cell Migration and Invasion in the Glioma Cell Lines U251, T98G, and SHG44 by Inhibiting the Wnt/β-Catenin Pathway.

Med Sci Monit 2018 Dec 15;24:9110-9119. Epub 2018 Dec 15.

Department of Neurosurgery, Suizhou Central Hospital, Suizhou, Hubei, China (mainland).

BACKGROUND The myosin heavy chain 10 or MYH10 gene encodes non-muscle myosin II B (NM IIB), and is involved in tumor cell migration, invasion, extracellular matrix (ECM) production, and epithelial-mesenchymal transition (EMT). This study aimed to investigate the effects of the MYH10 gene on normal human glial cells and glioma cell lines in vitro, by gene silencing, and to determine the signaling pathways involved. MATERIAL AND METHODS The normal human glial cell line HEB, and the glioma cell lines, U251, T98G, and SHG44 were studied. Read More

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December 2018
2 Reads

The DAHANCA 32 study: Electrochemotherapy for recurrent mucosal head and neck cancer.

Head Neck 2018 Dec 15. Epub 2018 Dec 15.

Department of Otorhinolaryngology, Head & Neck Surgery and Audiology, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark.

Background: Electrochemotherapy is an established treatment for cutaneous tumors. This study aimed at determining efficacy of electrochemotherapy in recurrent head and neck cancer.

Methods: Phase II clinical trial in patients with recurrent head and neck carcinomas with no curative treatment options. Read More

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December 2018
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