21,445 results match your criteria Gene[Journal]


Single and multi-locus association study of TCF7L2 gene variants with susceptibility to type 2 diabetes mellitus in an Iranian population.

Gene 2019 Feb 15. Epub 2019 Feb 15.

Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.

Prior studies indicated that some of transcription factor 7-like 2 (TCF7L2) gene variants such as rs7903146, rs12255372 and rs11255372 are constantly associated with Type 2 diabetes mellitus (T2DM) in various populations and ethnic groups. The purpose of this study was to assess the association between TCF7L2 variants (rs7903146, rs11196205, and rs11255372) and T2DM by TaqMan assay. Statistical analysis was performed through SNPAlyze and SPSS. Read More

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http://dx.doi.org/10.1016/j.gene.2019.01.040DOI Listing
February 2019

High-throughput transcriptome-Seq and small RNA-Seq reveal novel functional genes and microRNAs for early embryonic arrest in humans.

Gene 2019 Feb 15. Epub 2019 Feb 15.

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Guangxi Medical University, No.6 Shuangyong Road, Nanning, Guangxi, China. Electronic address:

Early Embryonic Arrest (EEA) is one of the major causes of female infertility. Genetic factors including specific genes and miRNAs may play pivotal roles on EEA. However, it is not well defined what genes and micro RNAs participate the pathophysiological alterations of EEA. Read More

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http://dx.doi.org/10.1016/j.gene.2018.12.084DOI Listing
February 2019

Mortalin is a distinct bio-marker and prognostic factor in serous ovarian carcinoma.

Gene 2019 Feb 15. Epub 2019 Feb 15.

Department of Pathology, Cancer Research Center, Yanbian University Medical College, Yanji 133002, China; Key Laboratory of the Science and Technology Department of Jilin Province, Yanji 133002, China. Electronic address:

This study focused on mortalin expression and its relevance to the prognosis in serous ovarian carcinoma, mortalin modulated cell malignant proliferation and EMT progression via Wnt/β-Catenin signaling pathway. In this study, data obtained from Oncomine database, Cancer Cell Line Encyclopedia (CCLE) analysis and Immunohistochemical (IHC) staining was used to assess the expression of mortalin in serous ovarian carcinoma. Using Kaplan-Meier plotter database and Kaplan-Meier analyzed the prognostic value of mortalin. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03781119193015
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http://dx.doi.org/10.1016/j.gene.2019.02.033DOI Listing
February 2019
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Construction of Parkinson's disease marker-based weighted protein-protein interaction network for prioritization of co-expressed genes.

Gene 2019 Feb 15. Epub 2019 Feb 15.

Karunya Institute of Technology and Sciences, Coimbatore, Tamil Nadu 641114, India; Zilkha Neurogenetic Institute, University of Southern California, Los Angeles, CA 90033, USA. Electronic address:

Background: Parkinson's disease (PD) is a complex neurodegenerative movement disorder that primarily results due to the loss of dopaminergic neurons in the substantia nigra region. Studying gene expression in the substantia nigra region would potentially unravel disease-relevant protein-protein interactions.

Methods: In this study we have used network science approach to prioritize candidate genes by focussing on differentially expressed genes (DEGs) that interact with established PD associated-genes (PDAG). Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.026DOI Listing
February 2019
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BrSKS13, a multiple-allele-inherited male sterility-related gene in Chinese cabbage (Brassica rapa L. ssp. pekinensis), affects pollen development and pollination/fertilization process.

Gene 2019 Feb 15. Epub 2019 Feb 15.

Department of Horticulture, Shenyang Agricultural University, Shenyang 110866, China. Electronic address:

Multiple-allele-inherited male sterility (MAMS) is important in Chinese cabbage (Brassica rapa L. ssp. pekinensis) breeding, but the molecular mechanisms leading to male sterility are poorly understood. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.031DOI Listing
February 2019

Identification and characterization of ABC transporters for carbohydrate uptake in Thermus thermophilus HB8.

Gene 2019 Feb 15. Epub 2019 Feb 15.

Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India. Electronic address:

Organisms use a variety of carbohydrates and metabolic pathways in order to capitalize in their specific environments. Depending upon their habitat, organism employs different types of transporters to maintain the cellular nutritional balance via central metabolism. A major contributor in this process in bacteria is a carbohydrate ABC transporter. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.035DOI Listing
February 2019

Genome-wide identification and characterization of HD-ZIP genes in potato.

Gene 2019 Feb 15. Epub 2019 Feb 15.

College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China. Electronic address:

HD-ZIP (Homeodomain leucine zipper) transcription factors play an important regulatory role in stress resistance in plants. The purpose of this study was to analyze the characteristics of the HD-ZIP genes/proteins and to study their expression profiles under high and low temperature conditions in potato (Solanum tuberosum L.). Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.024DOI Listing
February 2019

Curation and bioinformatic analysis of strabismus genes supports functional heterogeneity and proposes candidate genes with connections to RASopathies.

Gene 2019 Feb 14. Epub 2019 Feb 14.

Centre for Molecular Medicine and Therapeutics, BC Children's Hospital Research Institute, University of British Columbia, 950 West 28th Avenue, Vancouver, BC V5Z 4H4, Canada; Department of Medical Genetics, University of British Columbia, Vancouver, BC, Canada. Electronic address:

Strabismus refers to the misalignment of the eyes and occurs in 2-4% of individuals. The low-resolution label "strabismus" covers a range of heterogeneous defects, which makes it challenging to unravel this condition. Consequently a coherent understanding of the causes is lacking. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.020DOI Listing
February 2019

Overexpression of DNMT3A promotes proliferation and inhibits differentiation of porcine intramuscular preadipocytes by methylating p21 and PPARg promoters.

Gene 2019 Feb 14. Epub 2019 Feb 14.

Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address:

Intramuscular fat (IMF), which is modulated by the number and size of intramuscular preadipocytes, plays a key role in pork quality. DNA (cytosine-5)-methyltransferase 3A (DNMT3A), an enzyme that catalyzes the transfer of methyl groups to specific CpG structures in DNA, is involved in the management of diverse intracellular processes. However, the physiological functions of DNMT3A in proliferation and differentiation of porcine intramuscular preadipocytes have not been clearly established. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.029DOI Listing
February 2019

Neuroprotective effect of FMS-like tyrosine kinase-3 silence on cerebral ischemia/reperfusion injury in a SH-SY5Y cell line.

Gene 2019 Feb 14. Epub 2019 Feb 14.

Department of Neurology, Cangzhou Central Hospital, Cangzhou City, Hebei Province 061001, China.

Neuron damage contributes to ischemic brain injury. Although FMS-like tyrosine kinase-3 (FLT3) plays a critical role in neuron survival, its function and molecular mechanism in cerebral ischemia/reperfusion injury is unclear. In the present study, we exposed SH-SY5Y cells to oxygen and glucose deprivation/reoxygenation (OGD/R) to mimic ischemia/reperfusion injury. Read More

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http://dx.doi.org/10.1016/j.gene.2019.01.043DOI Listing
February 2019

Copy-number variation in goat genome sequence: A comparative analysis of the different litter size trait groups.

Gene 2019 Feb 14. Epub 2019 Feb 14.

College of Animal Science and Technology, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, Qingdao Agricultural University, Qingdao 266109, China; College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China. Electronic address:

Copy number variation (CNV), as an important component of genomic structural variation (SV), plays essential roles in phenotypic variability, disease susceptibility and species evolution. To investigate whether critical CNVs exist in dairy goats with differing fecundity, we performed genome-wide sequencing of two populations of Laoshan dairy goats with large differences in litter size. After reference genome aligning, CNV calling, and annotation, we screened identified CNVs in the high-fecundity (HF) and low-fecundity (LF) groups to identify discrepant CNVs and their distribution within the genome. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.027DOI Listing
February 2019

In vitro osteogenic differentiation potential of the human induced pluripotent stem cells augment when grown on graphene oxide-modified nanofibers.

Gene 2019 Feb 14. Epub 2019 Feb 14.

Department of Tissue engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address:

Due to the several limitations that surgeons are faced during bone tissue implantation there are daily increases in introducing new cell-co-polymer composites for use in bone tissue engineering approaches. In this study tried to develop a suitable nanostructured bio-composite for enhancing osteogenic differentiation of the human induced pluripotent stem cells (iPSCs). Polyvinylidene fluoride-Graphene oxide (PVDF-GO) nanofibers was fabricated by electrospinning and then characterized using scanning electron microscope, tensile and viability assays. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.028DOI Listing
February 2019

The inhibition of microRNA-203 on ischemic reperfusion injury after total knee arthroplasty via suppressing MYD88-mdiated toll-like receptor signaling pathway.

Gene 2019 Feb 14. Epub 2019 Feb 14.

Department of Orthopedics, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar 161000, PR China.

Ischemia reperfusion injury (IRI), a complex phenomenon often encountered in surgery, can lead to local and distant tissue destruction and sometimes even death. microRNA-203 (miR-203) has been reported to negatively regulate ischemia induced microglia activation with a feedback to myeloid differentiation primary-response gene 88 (MYD88). Accordingly, our study is to verify the effect of miR-203 and MYD88 on mice in IRI after total knee arthroplasty (TKA). Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.030DOI Listing
February 2019

RNA-binding protein RBM6 as a tumor suppressor gene represses the growth and progression in laryngocarcinoma.

Gene 2019 Feb 14. Epub 2019 Feb 14.

Department of Head and Neck Surgery, Hunan Cancer Hospital, No. 283, Tongzipo Road, Changsha City, Hunan Province, China. Electronic address:

Aberrant expression of RBM6 has been implicated in the development of human malignancies. However, the bio-function of RBM6 in laryngocarcinoma is still almost blank. Here we identified that RBM6 was downregulated in laryngocarcinoma tissues, as well as laryngocarcinoma cell lines. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.025DOI Listing
February 2019

Corrigendum to "Mir-202-3p regulates interleukin-1β-induced expression of matrix metalloproteinase 1 in human nucleus pulposus" [Gene 687 (2019) 156-165].

Gene 2019 Feb 13. Epub 2019 Feb 13.

Department of Bioengineering, University of Illinois at Chicago, IL, USA; Jesse Brown Veterans Affairs Medical Center (JBVAMC) at Chicago, IL, USA.

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http://dx.doi.org/10.1016/j.gene.2019.01.030DOI Listing
February 2019

Up-regulation of C5orf34 promotes lung adenocarcinoma migration and is correlated with worse prognosis.

Gene 2019 Feb 13. Epub 2019 Feb 13.

Department of Thoracic Surgery, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100021, China. Electronic address:

Objective: This study aimed to investigate the biological role of C5orf34 in Lung adenocarcinoma (LAD) and the mechanism of such role.

Methods: The mRNA expression of C5orf34 was analyzed using student's t-test based on the data obtained from TCGA database. Kaplan-Meier analysis was performed to evaluate the prognosis value of C5orf34. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.019DOI Listing
February 2019

Whole exome sequencing revealed mutations in FBXL4, UNC80, and ADKin Thai patients with severe intellectual disabilities.

Gene 2019 Feb 13. Epub 2019 Feb 13.

Center of Excellence for Medical Genomics, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand; Excellence Center for Medical Genetics, King Chulalongkorn Memorial Hospital, The Thai Red Cross Society, Bangkok 10330, Thailand.

Intellectual disabilities (ID) are etiologically heterogeneous. Advanced molecular techniques could be helpful in identification of the underlying genetic defects. We aimed to characterize clinical and molecular features of three Thai patients with ID. Read More

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http://dx.doi.org/10.1016/j.gene.2019.01.049DOI Listing
February 2019

Overexpression of TtNRAMP6 enhances the accumulation of Cd in Arabidopsis.

Gene 2019 Feb 12. Epub 2019 Feb 12.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China; Joint International Research Laboratory of Crop Resources and Genetic Improvement, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China. Electronic address:

The uptake and translocation of non-essential heavy metals in plant are always through metal transporters for essential micronutrient transport, such as NRAMP (Natural Resistance-Associated Macrophage Protein). NRAMPs from different species exhibit different biological functions, although their sequences are highly identical. In the present study, a NRAMP6 was isolated from Ailanmai (Triticum turgidum L. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03781119193011
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http://dx.doi.org/10.1016/j.gene.2019.02.008DOI Listing
February 2019
5 Reads

Exploring the involvement of Tac2 in the mouse hippocampal stress response through gene networking.

Gene 2019 Feb 12. Epub 2019 Feb 12.

Department of Genetics, Genomics and Informatics, University of Tennessee Health Science Center, Memphis, TN 38163, USA; Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China. Electronic address:

Tac2 is expressed in a number of areas throughout the brain, including the hippocampus. However, knowledge about its function has been only well explored in the hypothalamus in the context of reproductive health. In this study, we identified and validated increased hippocampal Tac2 mRNA expression in response to chronic mild stress in mice. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.013DOI Listing
February 2019

miR-3099 promotes neurogenesis and inhibits astrogliogenesis during murine neural development.

Gene 2019 Feb 12. Epub 2019 Feb 12.

Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. Electronic address:

MicroRNA-3099 is highly expressed during neuronal differentiation and development of the central nervous system. Here we characterised the role of miR-3099 during neural differentiation and embryonic brain development using a stable and regulatable mouse embryonic stem cell culture system for miR-3099 expression and in utero electroporation of miR-3099 expression construct into E15.5 embryonic mouse brains. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.014DOI Listing
February 2019

Chicken ZNF764L gene: mRNA expression profile, alternative splicing analysis and association analysis between first exon indel mutation and economic traits.

Gene 2019 Feb 12. Epub 2019 Feb 12.

College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China. Electronic address:

Zinc finger proteins are a class of transcription factors with finger-like domains and have diverse uses in biological processes, including development, differentiation, and metabolism. In this study, we identified the absence of the 24 bp sequence in the third exon of the zinc finger protein 764-like (ZNF764L) gene that lead to the production of two new transcripts, ZNF764L-SV1 and ZNF764L-SV2, and the sum of the expression levels of the two transcripts is approximately equal the total RNA expression level. Temporal and spatial expression showed that ZNF764L had higher expression during the embryonic stage. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.010DOI Listing
February 2019

Comparison in transcriptome and cytokine profiles of mesenchymal stem cells from human umbilical cord and cord blood.

Gene 2019 Feb 12. Epub 2019 Feb 12.

State key laboratory of bioelectronics, School of biological science & medical engineering, Southeast University, Nanjing, 210096, China. Electronic address:

Human umbilical cord (UC) and cord blood (CB) provide attractive sources of mesenchymal stem cells (MSCs) for cell therapy. Both UCMSCs and CBMSCs have been demonstrated to play prominent roles in clinical therapy. However, little is known about their functional differences in clinical application. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.017DOI Listing
February 2019
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Identifying mutation positions in all segments of influenza genome enables better differentiation between pandemic and seasonal strains.

Gene 2019 Feb 12. Epub 2019 Feb 12.

Department of Computer Science and Engineering, School of Electrical Engineering and Computer, Shiraz University, Shiraz, Iran; School of Animal and Veterinary Sciences, The University of Adelaide, Adelaide, Australia; Adelaide Medical School, Faculty of Health and Medical Sciences, The University of Adelaide, Adelaide, Australia; School of Information Technology and Mathematical Sciences, Division of Information Technology Engineering & Environment, University of South Australia, Adelaide, Australia; School of Biological Sciences, Faculty of Science and Engineering, Flinders University, Adelaide, Australia. Electronic address:

Influenza has a negative sense, single-stranded, segmented RNA. In the context of pandemic influenza research, most studies have focused on variations in the surface proteins (Hemagglutinin and Neuraminidase). However, new findings suggest that all internal and external proteins of influenza viruses can contribute in pandemic emergence, pathogenicity and increasing host range. Read More

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http://dx.doi.org/10.1016/j.gene.2019.01.014DOI Listing
February 2019

Association between polymorphisms of Xeroderma pigmentosum complementation group C gene (XPC) and susceptibility to schizophrenia.

Gene 2019 Feb 12. Epub 2019 Feb 12.

Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran. Electronic address:

Previous studies revealed that polymorphisms in several DNA repair genes are associated with the risk of schizophrenia. The relationship between three polymorphisms (Ala499Val, PAT, and Lys939Gln) of the XPC (MIM: 613208) and the risk of schizophrenia is investigated. A total of 361 schizophrenia cases and 360 healthy controls were included in the study. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.018DOI Listing
February 2019

Identification of a novel miR-206-Notch3 pathway regulating mouse myoblasts proliferation.

Gene 2019 Feb 11;695:57-64. Epub 2019 Feb 11.

Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:

MicroRNAs (miRNAs) are endogenous short non-coding RNAs and exert their function by targeting mRNAs of genes. miRNA-206 (miR-206) is exclusively expressed in adult skeletal muscles and plays an important role in myogenesis. However, the regulatory mechanisms of miR-206 in myoblasts proliferation and differentiation are still limited. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03781119193012
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http://dx.doi.org/10.1016/j.gene.2019.01.045DOI Listing
February 2019
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Genomic structural diversity in Criollo Argentino horses: Analysis of copy number variations.

Gene 2019 Feb 11;695:26-31. Epub 2019 Feb 11.

IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N Dulout" (UNLP CONICET LA PLATA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, 60 y 118 S/N, 1900 La Plata, Argentina. Electronic address:

Copy number variation (CNV) has been proved to be widespread in human, animal and plant genomes. Together with single nucleotide polymorphisms (SNPs), CVNs play a key role in genetic diversity. In this study, genome-wide detection of CNVs was performed based on SNP data from 24 Criollo Argentino horses genotyped with the GGP Equine70k array. Read More

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http://dx.doi.org/10.1016/j.gene.2018.12.067DOI Listing
February 2019

Adeno-associated virus 9-mediated RNA interference targeting SOCS3 alleviates diastolic heart failure in rats.

Gene 2019 Feb 11. Epub 2019 Feb 11.

Department of Cardiac Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China. Electronic address:

Objective: To explore the effect of adeno-associated virus 9-mediated RNA interference targeting SOCS3 (AAV9-SOCS3 siRNA) on the treatment of diastolic heart failure (DHF).

Method: A rat DHF model was established, and cardiac function and hemodynamic changes were measured. HE, Sirius red and TUNEL staining were applied to observe the pathological changes in the myocardium. Read More

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http://dx.doi.org/10.1016/j.gene.2019.01.044DOI Listing
February 2019

Long-term and high dose dexamethasone injection decreases the expression of Immunoglobulin Heavy (Light) Chain Variable Region Genes (IGH(L)Vs) in the mouse spleen.

Gene 2019 Feb 11;695:42-50. Epub 2019 Feb 11.

The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, The College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China. Electronic address:

Glucocorticoid hormones have been widely used in clinical practice as potent anti-inflammatory and immunosuppressive agents. However, the underlying mechanisms of how they work remain unaddressed. Here, we used RNA-set to profile spleen gene expression in adult mice after consistent intraperitoneal injection of dexamethasone. Read More

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http://dx.doi.org/10.1016/j.gene.2019.01.047DOI Listing
February 2019

MiRNA as biomarker for uveitis - A systematic review of the literature.

Gene 2019 Feb 11. Epub 2019 Feb 11.

Eye Hospital, University Medical Centre Ljubljana, Ljubljana, Slovenia; Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia. Electronic address:

Aim: A systematic review of miRNA profiling studies in uveitis.

Methods: Literature search strategy - Pubmed central central database, using miRNA/microRNA and intraocular inflammation/uveitis as keywords.

Results: We found twenty publications regarding the experimental and clinical use of miRNA in uveitis, published between 2011 and 2018. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03781119193010
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http://dx.doi.org/10.1016/j.gene.2019.02.004DOI Listing
February 2019
1 Read

Mutational analysis of CFTR in the Ecuadorian population using next-generation sequencing.

Gene 2019 Feb 11. Epub 2019 Feb 11.

Centro de Investigación Genética y Genómica, Facultad de Ciencias de la Salud, Universidad Tecnológica Equinoccial, Quito, Ecuador.

The frequency distributions of cystic fibrosis variants are heterogeneous in Ecuador because of the genetic admixture of its population. The aim of this study was to identify disease-causing variants among Ecuadorian cystic fibrosis (CF) patients by next-generation sequencing (NGS) of the entire cystic fibrosis transmembrane conductance regulator (CFTR) gene. The results showed an approximation of the frequencies of pathogenic variants in the population under study and an optimal mutation panel for routine first-line CF molecular diagnosis. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.015DOI Listing
February 2019
2.138 Impact Factor

Molecular characterization, cellular localization, and light-enhanced expression of Beta-Na/H Exchanger-like in the whitish inner mantle of the giant clam, Tridacna squamosa, denote its role in light-enhanced shell formation.

Gene 2019 Feb 11. Epub 2019 Feb 11.

Department of Biological Sciences, National University of Singapore, Kent Ridge, Singapore 117543, Republic of Singapore; The Tropical Marine Science Institute, National University of Singapore, Kent Ridge, Singapore 119227, Republic of Singapore. Electronic address:

The fluted giant clam, Tridacna squamosa, lives in symbiosis with photosynthetic zooxanthellae, and can engage in light-enhanced growth and shell formation. Light-enhanced shell formation necessitates the elimination of excess H from the extrapallial fluid adjacent to the shell. This study aimed to clone Na/HExchanger (NHE) from the whitish inner mantle adjacent to the extrapallial fluid of T. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.009DOI Listing
February 2019
2.138 Impact Factor

Characterization of a mutation in the zona pellucida module of Endoglin that causes hereditary hemorrhagic telangiectasia.

Gene 2019 Feb 11. Epub 2019 Feb 11.

Molecular Medicine Department, General Biology and Medical Genetics Unit, University of Pavia, Via Forlanini 14, 27100 Pavia, Italy. Electronic address:

Hereditary hemorrhagic telangiectasia (HHT) is a vascular rare disease characterized by nose and gastrointestinal bleeding, skin and mucosa telangiectasias, and arteriovenous malformations in internal organs. HHT shows an autosomal dominant inheritance and a worldwide prevalence of approximately 1:5000 individuals. In >80% of patients, HHT is caused by mutations in either ENG (HHT1) or ACVRL1 (HHT2) genes, which code for the membrane proteins Endoglin and Activin A Receptor Type II-Like Kinase 1 (ALK1), respectively, both belonging to the TGF-β/BMP signaling pathway. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03781119193012
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http://dx.doi.org/10.1016/j.gene.2019.02.016DOI Listing
February 2019
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Stable G-quadruplex enabling sequences are selected against by the context-dependent codon bias.

Gene 2019 Feb 9. Epub 2019 Feb 9.

Department of Chemistry and Biochemistry, Kent State University, Kent, OH 44242, United States of America. Electronic address:

The distributions of secondary structural elements appear to differ between coding regions (CDS) of mRNAs compared to the untranslated regions (UTRs), presumably as a mechanism to fine-tune gene expression, including efficiency of translation. However, a systematic and comprehensive analysis of secondary structure avoidance because of potential bias in codon usage is difficult as some of the common secondary structures, such as, hairpins can be formed by numerous sequence combinations. Using G-quadruplex (GQ) as the model secondary structure we studied the impact of codon bias on GQs within the CDS. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.006DOI Listing
February 2019
2 Reads

Molecular cloning and functional characterization of elongase (elovl5) and fatty acyl desaturase (fads2) in sciaenid, Nibea diacanthus (Lacepède, 1802).

Gene 2019 Feb 10;695:1-11. Epub 2019 Feb 10.

Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou 515063, PR China. Electronic address:

In the present paper, we investigated the molecular cloning and functional characterization of elongase of very long chain fatty acid (elovl) and fatty acyl desaturase (fads) genes in a marine teleost, Nibea diacanthus. The elongase cDNA sequence encoded a polypeptide of 294 amino acids exhibiting Elovl5 activity, which effectively elongated both C18 (18:2n-6, 18:3n-3 and 18:3n-6) and C20 (20:4n-6 and 20:5n-3) polyunsaturated fatty acids. The desaturase cDNA sequence specified a polypeptide of 445 amino acids indicating Δ6 desaturation activity, which coul converted C18:2n-6 and C18:3n-3 to C18:3n-6 and C18:4n-3, respectively. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03781119193009
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http://dx.doi.org/10.1016/j.gene.2019.01.033DOI Listing
February 2019
1 Read
2.138 Impact Factor

Spectrum of clinical heterogeneity of β-tubulin TUBB5 gene mutations.

Gene 2019 Feb 7;695:12-17. Epub 2019 Feb 7.

Biochemistry and Molecular Genetics Department, Hospital Clínic and IDIBAPS, Barcelona, Spain; Centre for Biomedical Research on Rare Diseases (CIBERER), ISCIII, Barcelona, Spain.

Microcephaly is a rare condition in which the occipitofrontal circumference in a child is more than two standard deviations below the mean of children of the same age and gender. It is mainly caused by genetic abnormalities that interfere with the growth of the cerebral cortex during early months of fetal development. We present a case of a 12 years old patient with microcephaly. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03781119193010
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http://dx.doi.org/10.1016/j.gene.2019.02.002DOI Listing
February 2019
1 Read

The fitness consequences of synonymous mutations in Escherichia coli: Experimental evidence for a pleiotropic effect of translational selection.

Gene 2019 Feb 8;694:111-120. Epub 2019 Feb 8.

Department of Biology, American University, 4400 Massachusetts Avenue, NW, Washington, DC, 20016, United States of America. Electronic address:

Codon usage bias (CUB) is a universal feature of genomes, and in most species CUB of protein coding genes is positively correlated with expression level and degree of evolutionary conservation. There is mounting experimental evidence that CUB is due in part to selection for translational efficiency and/or accuracy, i.e. Read More

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http://dx.doi.org/10.1016/j.gene.2019.01.031DOI Listing
February 2019

Highly conserved epitopes of DENV structural and non-structural proteins: Candidates for universal vaccine targets.

Gene 2019 Feb 8;695:18-25. Epub 2019 Feb 8.

Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110007, India.

Dengue is a severe emerging arthropod borne viral disease occurring globally. Around two fifths of the world's population, or up to 3.9 billion people, are at a risk of dengue infection. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.001DOI Listing
February 2019

Chromosomal and molecular characterization of 5S rRNA genes in the North American abalones Haliotis rufescens Swainson (red abalone) and H. fulgens Philippi (blue abalone).

Gene 2019 Feb 8;695:65-74. Epub 2019 Feb 8.

Aquaculture Genetics & Breeding Laboratory, Centro de Investigaciones Biológicas del Noroeste, Av. Instituto Politécnico Nacional 195, Col. Playa Palo de Sta. Rita, La Paz, BCS 23096, Mexico. Electronic address:

Abalone is an extremely valuable food source derived from cultured and wild animals, the later from populations under intense fishing exploitation and of high conservation value. As part of a long-term study to characterize genes from abalone that can be used as markers for hybrids certification, we characterised 5S ribosomal DNA (5S rDNA) in red abalone (Haliotis rufescens) and blue abalone (H. fulgens). Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.003DOI Listing
February 2019

Two ICE isoforms showing differential transcriptional regulation by cold and hormones participate in Brassica juncea cold stress signaling.

Gene 2019 Feb 8;695:32-41. Epub 2019 Feb 8.

Molecular Plant Physiology and Proteomics Laboratory, Department of Botany, University of Delhi, India. Electronic address:

C-repeat binding factor (CBF) dependent cold stress signaling cascade is well studied in the model plant arabidopsis but is relatively lesser studied in the crop plants. In the present study, two novel isoforms of an upstream regulator of CBF, Inducer of CBF expression (ICE), BjICE46 (1314 bp, accession number HQ446510) and BjICE53 (1494 bp, accession number HQ857208) were isolated from Brassica juncea seedlings. Genomic clones of both the isoforms (accession numbers HQ433510 and JX571043) showed three introns, out of which one intron was spanning the bHLH (basic helix-loop-helix) domain. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.005DOI Listing
February 2019

Association and interaction of genetic variants with occurrence of ischemic stroke among Brazilian patients.

Gene 2019 Feb 7. Epub 2019 Feb 7.

University of Region of Joinville, UNIVILLE, Joinville Stroke Registry, Joinville, Brazil.

Ischemic Stroke (IS) is a severe and complex disorder of high morbidity and mortality rates associated with clinical, environmental, and genetic predisposing factors. Despite previous studies have associated genetic variants to stroke, inconsistent results from different populations pointed to the genetic heterogeneity for IS. Therefore, we may hypothesize that an interaction effect among genetic variants could contribute to IS occurrence rather than genetic variants independently. Read More

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http://dx.doi.org/10.1016/j.gene.2019.01.041DOI Listing
February 2019

Single nucleotide polymorphism in PTEN-Long gene: A risk factor in chronic myeloid leukemia.

Gene 2019 Feb 7;694:71-75. Epub 2019 Feb 7.

Laboratorio de Genética Hematológica, IMEX, CONICET-Academia Nacional de Medicina, Buenos Aires, Argentina.

The BCR-ABL1 oncogene is associated with chronic myeloid leukemia (CML) pathogenesis, but the molecular mechanisms that initiate leukemogenesis are still unclear. Cancer pathogenesis has been associated with genetic alterations that may lead to inactivation of tumor suppressor genes. Phosphatase and tensin homolog (PTEN) is frequently deleted or inactivated in various tumors. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03781119193009
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http://dx.doi.org/10.1016/j.gene.2019.01.038DOI Listing
February 2019
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Maternal stress during pregnancy and small for gestational age birthweight are not associated with telomere length at 11 years of age.

Gene 2019 Feb 7;694:97-101. Epub 2019 Feb 7.

Department of Medicine, University of Auckland, Auckland 1142, New Zealand.

Background: Previous studies indicate that low birth weight and exposure to maternal stress during pregnancy may result in shortened telomeres in infants. Shorter telomere length has in turn been linked with accelerated ageing and with age-related diseases. This study aimed to investigate the association between pregnancy and birth factors and relative telomere length in offspring at 11 years of age. Read More

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http://dx.doi.org/10.1016/j.gene.2019.01.017DOI Listing
February 2019

Polymorphisms of IGF-IR gene and their association with economic traits in two indigenous Chinese dairy goat breeds.

Gene 2019 Feb 7;695:51-56. Epub 2019 Feb 7.

State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China. Electronic address:

The insulin like growth factor 1 receptor (IGF-IR) plays an important role in regulating growth and development. To investigate the effects of IGF-IR polymorphisms on the economic traits of dairy goats, polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing methods were used to screen single nucleotide polymorphisms (SNPs) within 9 IGF-IR fragments in Xinong Saanen dairy goat (XS, n = 268) and Guanzhong dairy goat (GZ, n = 440). Consequently, two SNPs, including NC_007319: g. Read More

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http://dx.doi.org/10.1016/j.gene.2019.01.039DOI Listing
February 2019

Expression of the lncRNA ZFAS1 in cervical cancer and its correlation with prognosis and chemosensitivity.

Gene 2019 Feb 7. Epub 2019 Feb 7.

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University, Suzhou 215100, Jiangsu, China. Electronic address:

Objective: To investigate the expression of the lncRNA ZFAS1 in cervical cancer and its relationship with patient prognosis and cervical cancer cell chemosensitivity.

Methods: The expression of ZFAS1 in cervical cancer tissues and cell lines was detected by qRT-PCR. The cervical cancer CaSki and the HeLa cell lines were transfected to be divided into Blank, siR-Control, and siR-ZFAS1 groups. Read More

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http://dx.doi.org/10.1016/j.gene.2019.01.025DOI Listing
February 2019

Large-scale mitochondrial gene rearrangements in the hermit crab Pagurus nigrofascia and phylogenetic analysis of the Anomura.

Gene 2019 Feb 6. Epub 2019 Feb 6.

National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Zhejiang Ocean University, 316022 Zhoushan, China; National Engineering Research Center for Facilitated Marine Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, 316022 Zhoushan, China.

Complete mitochondrial genome (mitogenome) provides important information for better understanding of gene rearrangement, molecular evolution and phylogenetic analysis. Currently, only a few Paguridae mitogenomes have been reported. Herein, we described the complete mitogenome of hermit crab Pagurus nigrofascia. Read More

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http://dx.doi.org/10.1016/j.gene.2019.01.035DOI Listing
February 2019

Genetic variants as risk factors for cigarette smoking at an early age and relapse to smoking cessation treatment: A pilot study.

Gene 2019 Feb 6;694:93-96. Epub 2019 Feb 6.

HLA Laboratory, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, Mexico. Electronic address:

Objectives: Tobacco smoking is a complex and multifactorial disease involving both environmental and genetic factors. In the Mexican mestizo population, single-nucleotide polymorphisms (SNPs) associated with cigarette smoking and a greater degree of nicotine addiction have been identified; however, no possible roles have been explored in regard to the age of onset of smoking or in the success of quitting.

Methods: In this study, 151 Mexican mestizo, who smoke cigarettes, were included. Read More

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http://dx.doi.org/10.1016/j.gene.2019.01.036DOI Listing
February 2019
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Long non-coding antisense RNA GAS6-AS1 supports gastric cancer progression via increasing GAS6 expression.

Gene 2019 Feb 5. Epub 2019 Feb 5.

Department of Gastrointestinal Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang Province, PR China. Electronic address:

Objective: As one broader class of non-coding RNAs (lncRNAs), non-coding antisense (AS) transcripts are functionally characterized to play pivotal roles in various pathophysiological processes, including tumor biology.

Methods: In this study, the exact biological functions and regulation mechanisms of GAS6-AS1 in gastric cancer (GC) was examined.

Results: The expression of GAS6-AS1 was markedly upregulated in GC tissues and is associated with advanced stage (III + IV) of GC patients. Read More

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http://dx.doi.org/10.1016/j.gene.2018.12.079DOI Listing
February 2019
2.138 Impact Factor

Bladder smooth muscle cell differentiation of the human induced pluripotent stem cells on electrospun Poly(lactide-co-glycolide) nanofibrous structure.

Gene 2019 Feb 6;694:26-32. Epub 2019 Feb 6.

Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address:

Smooth muscle cell (SMC) regeneration plays an important role in retrieving the bladder-wall functionality and it can be achieved by a proper cell-co-polymer constructed by tissue engineering. Human induced pluripotent stem cells (iPSCs), which can be specifically prepared for the patient, was considered as cells in this study, and Poly(lactide-co-glycolide) (PLGA) as a most interesting polymer in biomedical applications was applied to the scaffold fabrication by electrospinning. After scaffold characterization, SMC differentiation potential of the human iPSCs was investigated while cultured on the PLGA nanofibrous scaffold by evaluation of the SMC related important gene and protein markers. Read More

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http://dx.doi.org/10.1016/j.gene.2019.01.037DOI Listing
February 2019

Circulating leptin concentration, LEP gene variants and haplotypes, and polycystic ovary syndrome in Bahraini and Tunisian Arab women.

Gene 2019 Feb 2;694:19-25. Epub 2019 Feb 2.

Faculty of Sciences, El Manar University, Tunis, Tunisia; School of Medicine, Nazarbayev University, Astana, Kazakhstan. Electronic address:

Background And Aim: Epidemiological studies suggested that ethnic/racial background influences the associations of altered leptin secretion and leptin gene (LEP) polymorphisms with polycystic ovary syndrome (PCOS). We investigated the association between LEP variants and plasma leptin levels with PCOS in Tunisian and Bahraini Arab women.

Subjects And Methods: Retrospective case-control study, involving 255 PCOS cases and 253 control subjects from Bahrain, and 320 women PCOS cases and 447 controls from Tunisia. Read More

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http://dx.doi.org/10.1016/j.gene.2019.01.032DOI Listing
February 2019
1 Read
2.138 Impact Factor

MicroRNA-185 inhibits proliferation, migration and invasion in human osteosarcoma MG63 cells by targeting vesicle-associated membrane protein 2.

Gene 2019 Feb 2. Epub 2019 Feb 2.

Department of Orthopedics, Zhoukou Cetral Hospital, Zhoukou City, Henan Province, 466000, PR China.

MicroRNAs (miRNAs) play an essential role in cancer development. Several studies have indicated that miRNAs mediate tumorigenesis processes, such as inflammation, proliferation, apoptosis and invasion. In the present study, we aimed to investigate the role of the microRNA-185 (miR-185) on the proliferation, migration and invasion of osteosarcoma (OS). Read More

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http://dx.doi.org/10.1016/j.gene.2019.01.034DOI Listing
February 2019
1 Read