11,694 results match your criteria Gastroenteritis Viral


Group A rotavirus surveillance before vaccine introduction in Italy, September 2014 to August 2017.

Euro Surveill 2019 Apr;24(15)

The members of the RotaNet-Italy Study Group who contributed data are listed at the end of this article.

IntroductionGroup A rotaviruses (RVA) are the leading cause of acute gastroenteritis (AGE) in young children, causing ca 250,000 deaths worldwide, mainly in low-income countries. Two proteins, VP7 (glycoprotein, G genotype) and VP4 (protease-sensitive protein, P genotype), are the basis for the binary RVA nomenclature. Although 36 G types and 51 P types are presently known, most RVA infections in humans worldwide are related to five G/P combinations: G1P[8], G2P[4], G3P[8], G4P[8], G9P[8]. Read More

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http://dx.doi.org/10.2807/1560-7917.ES.2019.24.15.1800418DOI Listing

Improvement of Rotavirus Genotyping Method by Using the Semi-Nested Multiplex-PCR With New Primer Set.

Front Microbiol 2019 29;10:647. Epub 2019 Mar 29.

Laboratory of Viral Infection I, Department of Infection Control and Immunology, Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.

Rotavirus A (RVA) is a major cause of gastroenteritis in infants and young children. After vaccine introduction, RVA surveillance has become more important for monitoring changes in genotype distribution, and the semi-nested multiplex-PCR is a popular method for RVA genotyping. In particular, the VP7 primer set reported by Gouvea and colleagues in 1990 is still widely used worldwide as the recommended WHO primer set in regional and national reference RVA surveillance laboratories. Read More

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http://dx.doi.org/10.3389/fmicb.2019.00647DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449864PMC

Norovirus Detection at Oualidia Lagoon, a Moroccan Shellfish Harvesting Area, by Reverse Transcription PCR Analysis.

Food Environ Virol 2019 Apr 13. Epub 2019 Apr 13.

Marine Biotechnology and Environment Laboratory, Faculty of Sciences, University Chouaib Doukkali, El Jadida, Morocco.

Norovirus (NoV) is the leading cause of acute viral gastroenteritis outbreaks in the world. These outbreaks are frequently associated with bivalve shellfish consumption, particularly because these products are often eaten raw or only slightly cooked. In Morocco, regulations concerning the acceptable levels of enteric bacteria indicator organisms in these products have been put in place. Read More

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http://dx.doi.org/10.1007/s12560-019-09386-0DOI Listing

Epidemiology of Norovirus Outbreaks Reported to the Public Health Emergency Event Surveillance System, China, 2014⁻2017.

Viruses 2019 Apr 11;11(4). Epub 2019 Apr 11.

Division of Infectious Disease, Key Laboratory of Surveillance and Early-warning on Infectious Disease, Chinese Centre for Disease Control and Prevention, Beijing 102206, China.

We conducted a retrospective analysis of norovirus outbreaks reported to the National Public Health Emergency Event Surveillance System (PHEESS) in China from January 1, 2014 to December 31, 2017. We reviewed all acute gastroenteritis outbreaks ( = 692) submitted to PHEESS to identify the frequency, seasonality, geographic distribution, setting, and transmission mode of outbreaks due to norovirus. A total of 616 norovirus outbreaks resulting in 30,848 cases were reported. Read More

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http://dx.doi.org/10.3390/v11040342DOI Listing
April 2019
2 Reads

Whole genome characterization of feline-like G3P[8] reassortant rotavirus A strains bearing the DS-1-like backbone genes detected in Vietnam, 2016.

Infect Genet Evol 2019 Apr 9;73:1-6. Epub 2019 Apr 9.

Department of Hygiene and Molecular Epidemiology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.

While conducting rotavirus gastroenteritis surveillance in Vietnam, two G3P[8] rotavirus A specimens possessing an identical short RNA electropherotype were detected. They were RVA/Human-wt/VNM/0232/2016/G3P[8] and RVA/Human-wt/VNM/0248/2016/G3P[8], and recovered from 9 and 23 months old boys, respectively. The patients developed diarrhoea within one-week interval in March 2016 but in places >100 km apart in northern Vietnam. Read More

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http://dx.doi.org/10.1016/j.meegid.2019.04.007DOI Listing
April 2019
1 Read

Norovirus Infections and Disease in Lower-MiddleandLow-Income Countries, 1997⁻2018.

Authors:
Janet Mans

Viruses 2019 Apr 10;11(4). Epub 2019 Apr 10.

Department of Medical Virology, Faculty of Health Sciences, University of Pretoria,Pretoria 0001, South Africa.

Noroviruses are a major cause of viral gastroenteritis. The burden of the norovirus in lowresourcesettings is not well-established due to limited data. This study reviews the norovirusprevalence, epidemiology, and genotype diversity in lower-middle-income countries (LMIC) andin low-income countries (LIC). Read More

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https://www.mdpi.com/1999-4915/11/4/341
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http://dx.doi.org/10.3390/v11040341DOI Listing
April 2019
7 Reads

Histo-Blood Group Antigens in Children with Symptomatic Rotavirus Infection.

Viruses 2019 Apr 10;11(4). Epub 2019 Apr 10.

Department of Microbiology, School of Medicine, University of Valencia and Clinical Microbiology Service, Hospital Clínico Universitario de Valencia, Instituto de Investigación INCLIVA, 46010 Valencia, Spain.

Group A rotaviruses are a major cause of acute gastroenteritis in children. The diversity and unequal geographical prevalence of rotavirus genotypes have been linked to histo-blood group antigens (HBGAs) in different human populations. In order to evaluate the role of HBGAs in rotavirus infections in our population, secretor status (FUT2+), ABO blood group, and Lewis antigens were determined in children attended for rotavirus gastroenteritis in Valencia, Spain. Read More

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http://dx.doi.org/10.3390/v11040339DOI Listing
April 2019
1 Read

Characterization of Novel Reoviruses [Wad Medani virus (Orbivirus) and Kundal (Coltivirus)] collected from ticks in India during CCHF surveillance.

J Virol 2019 Apr 10. Epub 2019 Apr 10.

ICMR-National Institute of Virology, Pune, India

In 2011, ticks were collected from livestock following an outbreak of Crimean Congo Hemorrhagic fever (CCHF) in Gujarat state, India. CCHF-negative tick pools were passaged for virus isolation, and two virus isolates were obtained, designated Karyana virus (KARYV) and Kundal virus (KUNDV) respectively. Traditional RT-PCR identification of known viruses was unsuccessful, but a next-generation sequencing approach identified KARYV and KUNDV as viruses in the family, , and genera, respectively. Read More

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http://dx.doi.org/10.1128/JVI.00106-19DOI Listing
April 2019
1 Read

Cholesterol 25-hydroxylase negatively regulates porcine intestinal coronavirus replication by the production of 25-hydroxycholesterol.

Vet Microbiol 2019 Apr 6;231:129-138. Epub 2019 Mar 6.

Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China; Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address:

Cholesterol 25-hydroxylase (CH25H) has been shown lately to be a host restriction factor that encodes an enzyme, which catalyzes the oxidized form of cholesterol to 25-hydroxycholesterol (25HC). A series of studies have shown that 25HC activity in hosts plays a vital role in inhibiting viral infection. In this study, we explored the antiviral effect of CH25H and 25HC on porcine epidemic diarrhea virus (PEDV), which causes high mortality rates in newborn piglets with severe diarrhea, and considerable financial loss in the swine industry worldwide. Read More

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http://dx.doi.org/10.1016/j.vetmic.2019.03.004DOI Listing
April 2019
1 Read

A multiplex RT-PCR assay for rapid and simultaneous detection of four RNA viruses in swine.

J Virol Methods 2019 Apr 2;269:38-42. Epub 2019 Apr 2.

State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan 430072, China. Electronic address:

A multiplex reverse transcription polymerase chain rection (mRT-PCR) was developed for simultaneous detection of four RNA viruses in swine. The conserved target sequences directed to classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis coronavirus (TGEV) were selected based on alignments of genomic sequences and then specific primers were designed. The mRT-PCR assay was developed and evaluated for its specificity and sensitivity. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934193002
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http://dx.doi.org/10.1016/j.jviromet.2019.04.001DOI Listing
April 2019
5 Reads

Active Surveillance for Norovirus in a US Veterans Affairs Patient Population, Houston, Texas, 2015-2016.

Open Forum Infect Dis 2019 Apr 6;6(4):ofz115. Epub 2019 Mar 6.

National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia.

Background: Norovirus is a leading cause of acute gastroenteritis (AGE); however, few data exist on endemic norovirus disease burden among adults. Candidate norovirus vaccines are currently in development for all ages, and robust estimates of norovirus incidence among adults are needed to provide baseline data.

Methods: We conducted active surveillance for AGE among inpatients at a Veterans Affairs (VA) hospital in Houston, Texas. Read More

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http://dx.doi.org/10.1093/ofid/ofz115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441783PMC
April 2019
2 Reads

Antibiotic prescription among outpatients in a prefecture of Japan, 2012-2013: a retrospective claims database study.

BMJ Open 2019 Apr 3;9(4):e026251. Epub 2019 Apr 3.

Division of General Internal Medicine, Jichi Medical University Hospital, Shimotsuke, Tochigi, Japan.

Objectives: To investigate oral antibiotic prescribing patterns and identify factors associated with antibiotic prescriptions, with the aim of guiding future interventions to reduce inappropriate prescribing.

Design: Retrospective cohort study.

Setting: Database of public health insurance claims in Kumamoto prefecture (Japan). Read More

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http://dx.doi.org/10.1136/bmjopen-2018-026251DOI Listing
April 2019
2 Reads

Multiplex detection of six swine viruses on an integrated centrifugal disk using loop-mediated isothermal amplification.

J Vet Diagn Invest 2019 Apr 4:1040638719841096. Epub 2019 Apr 4.

Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing, China (Yuan, Deng, C Wang, Lv, Lin, Wu).

Advances in molecular testing and microfluidic technologies have opened new avenues for rapid detection of animal viruses. We used a centrifugal microfluidic disk (CMFD) to detect 6 important swine viruses, including foot-and-mouth disease virus, classical swine fever virus, porcine reproductive and respiratory swine virus-North American genotype, porcine circovirus 2, pseudorabies virus, and porcine parvovirus. Through integrating the loop-mediated isothermal amplification (LAMP) method and microfluidic chip technology, the CMFD could be successfully performed at 62℃ in 60 min. Read More

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http://dx.doi.org/10.1177/1040638719841096DOI Listing
April 2019
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No evidence of increase in the incidence of norovirus gastroenteritis hospitalizations in young children after the introduction of universal rotavirus immunization in Israel.

Hum Vaccin Immunother 2019 Apr 4. Epub 2019 Apr 4.

a Department of Epidemiology and Preventive Medicine, School of Public Health, Sackler Faculty of Medicine , Tel Aviv University , Ramat Aviv, Tel Aviv , Israel.

Following the introduction of universal immunization against rotavirus, concerns were raised regarding pathogen-replacement of rotavirus by norovirus. The study aim was to examine the incidence and characteristics and norovirus gastroenteritis before and after the introduction of universal rotavirus immunization in Israel. We studied 1179 stool samples collected between November 2007 and December 2014 for a prospective hospital-based surveillance study of children aged 0-59 months hospitalized for gastroenteritis. Read More

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https://www.tandfonline.com/doi/full/10.1080/21645515.2019.1
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http://dx.doi.org/10.1080/21645515.2019.1599522DOI Listing
April 2019
4 Reads

Hepatobiliary and pancreatic manifestations in inflammatory bowel diseases: a referral center study.

BMC Gastroenterol 2019 Apr 3;19(1):48. Epub 2019 Apr 3.

Department of Gastroenterology and Hepatology, Medical school and University of Ioannina, Ioannina, Greece.

Background: Hepatobiliary and pancreatic manifestations have been reported in patients with Crohn's disease or ulcerative colitis. Our aim was to describe the prevalence of hepatobiliary and pancreatic manifestations in inflammatory bowel disease and their association with the disease itself and the medications used.

Methods: Data were retrospectively extracted from the clinical records of patients followed up at our tertiary IBD referral Center. Read More

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http://dx.doi.org/10.1186/s12876-019-0967-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446300PMC
April 2019
2 Reads

Molecular detection of human adenovirus in urban wastewater in Egypt and among children suffering from acute gastroenteritis.

J Water Health 2019 Apr;17(2):287-294

Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, Egypt.

Incidence of enteric viruses in sewage, the efficacy of wastewater treatment plants to remove these viruses, and health effects from their release into the surface water are very important environmental issues in the microbiology field. One of the most pathogenic enteric viruses is adenovirus which can cause a serious disease such as gastroenteritis with low grade fever and mild dehydration in humans. In this study we performed qualitative polymerase chain reaction (PCR) analysis of HAdV on 60 stool samples from children with acute gastroenteritis admitted to Abu-Rish hospital and 96 environmental samples (32 raw sewage, 32 treated sewage, 32 sewage sludge) collected from Zenin wastewater treatment plant (WWTP). Read More

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http://dx.doi.org/10.2166/wh.2019.303DOI Listing

[Acute Viral Gastroenteritis: Viruses Other Than Norovirus].

Authors:
Andrea Zbinden

Praxis (Bern 1994) 2019 ;108(5):335-339

1 Institut für Medizinische Virologie, Universität Zürich.

Acute Viral Gastroenteritis: Viruses Other Than Norovirus Abstract. Norovirus is the leading cause of acute viral gastroenteritis. Norovirus is highly contagious, thus outbreaks of norovirus in hospitals and long-term care facilities are feared. Read More

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http://dx.doi.org/10.1024/1661-8157/a003182DOI Listing
January 2019
1 Read

The Effect of Rotavirus Vaccine on Socioeconomic Differentials of Paediatric Care Due to Gastroenteritis in Swedish Infants.

Int J Environ Res Public Health 2019 Mar 27;16(7). Epub 2019 Mar 27.

Sachs' Children and Youth Hospital, South General Hospital, 118 83 Stockholm, Sweden.

: Previous Swedish studies have shown a social gradient on paediatric care for viral gastroenteritis. : To study the effect of a free rotavirus vaccine programme on hospital care for viral gastroenteritis. : A register-based national cohort study of paediatric in- and outpatient care for viral gastroenteritis in children <2 years old in two Swedish counties in 2014⁻2017, with the rest of the country as comparison. Read More

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http://dx.doi.org/10.3390/ijerph16071095DOI Listing
March 2019
2 Reads

Discovery of Cucumis melo endornavirus by deep sequencing of human stool samples in Brazil.

Virus Genes 2019 Mar 26. Epub 2019 Mar 26.

Enteric Disease Laboratory, Virology Center, Adolfo Lutz Institute, Av. Dr Arnaldo, no 355, São Paulo, São Paulo, 01246-902, Brazil.

The nearly complete genome sequences of two Cucumis melo endornavirus (CmEV) strains were obtained using deep sequencing while investigating fecal samples for the presence of gastroenteritis viruses. The Brazilian CmEV BRA/TO-23 (aa positions 116-5027) and BRA/TO-74 (aa positions 26-5057) strains were nearly identical to the reference CmEV CL-01 (USA) and SJ1 (South Korea) strains, showing 97% and 98% of nucleotide and amino acid identity, respectively. Endornaviruses are not known to be associated with human disease and their presence may simply reflect recent dietary consumption. Read More

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http://dx.doi.org/10.1007/s11262-019-01648-0DOI Listing
March 2019
1 Read

Upregulation of CHOP participates in caspase activation and virus release in human astrovirus-infected cells.

J Gen Virol 2019 Mar 26. Epub 2019 Mar 26.

4​Laboratory of Radiation Safety, National Center for Geriatrics and Gerontology, Obu, Aichi, 474-8522, Japan.

Human astroviruses (HAstVs), non-enveloped RNA viruses with positive-sense RNA genomes, are an important cause of acute gastroenteritis in young children, although the processes that produce infectious virions are not clearly defined. To track the viral replication complex (RC) upon HAstV1 infection, the subcellular distribution of double-stranded (ds) RNA and of ORF1b, a viral RNA polymerase, was examined by immunocytochemistry. Foci that were positive for dsRNA and for ORF1b were co-localized, and both foci were also co-localized with resident proteins of the endoplasmic reticulum (ER). Read More

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http://dx.doi.org/10.1099/jgv.0.001250DOI Listing
March 2019
2 Reads
3.183 Impact Factor

Performance evaluation of gastrointestinal viral ELIte panel multiplex RT-PCR assay for the diagnosis of rotavirus, adenovirus and astrovirus infection.

J Virol Methods 2019 Jun 19;268:48-52. Epub 2019 Mar 19.

Dpt di Promozione della Salute, Materno infantile, Medicina Interna e Specialistica di eccellenza "G.D'Alessandro", Università di Palermo, Italy.

Rotavirus, adenovirus, norovirus and astrovirus are considered to be among the major causes of sporadic cases and outbreaks of acute gastroenteritis globally. Rapid and accurate identification of enteric viruses is still a challenge for the clinical laboratory. Recently, several molecular platforms for the detection of viral enteric pathogens have become available. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.03.010DOI Listing
June 2019
2 Reads

Occurrence of Salivirus in Sewage and River Water Samples in Karaj, Iran.

Food Environ Virol 2019 Mar 20. Epub 2019 Mar 20.

Department of Research and Development, Production and Research Complex, Pasteur Institute of Iran, Tehran, Iran.

Salivirus is a newly discovered virus which seems to be related to acute gastroenteritis in children. Salivirus may infect susceptible children by fecal-oral route after exposure to contaminated water. The present study aims to evaluate the occurrence and quantity of Salivirus in treated and untreated sewage water and river water samples collected in the city of Karaj, Iran by reverse transcription-quantitative PCR assay. Read More

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http://dx.doi.org/10.1007/s12560-019-09377-1DOI Listing
March 2019
2 Reads

Norovirus and rotavirus infections in children less than five years of age hospitalized with acute gastroenteritis in Indonesia.

Arch Virol 2019 Mar 18. Epub 2019 Mar 18.

Department of Microbiology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.

Rotaviruses and noroviruses are the most important viral causes of acute gastroenteritis in children. While previous studies of acute gastroenteritis in Indonesia mainly focused on rotavirus, here, we investigated the burden and epidemiology of norovirus and rotavirus disease. Children less than five years of age hospitalized with acute gastroenteritis were enrolled in this study from January to December 2015 at three participating hospitals. Read More

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http://dx.doi.org/10.1007/s00705-019-04215-yDOI Listing
March 2019
3 Reads
2.282 Impact Factor

Efficient recovery and enrichment of infectious rotavirus using separation with antibody-integrated graphite-encapsulated magnetic nanobeads produced by argon/ammonia gas plasma technology.

Int J Nanomedicine 2019 12;14:1865-1876. Epub 2019 Mar 12.

Research Institute of Electronics, Shizuoka University, Hamamatsu, Shizuoka 432-8561, Japan.

Background: Rotavirus is the representative cause of severe acute gastroenteritis in young children. A characteristic feature of rotavirus is low infectious dose and robustness of the virion, suggesting sanitation and hygiene will have little impact. Thus, development of a vaccine should be given priority. Read More

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http://dx.doi.org/10.2147/IJN.S191784DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419588PMC
March 2019
1 Read

Prevalence and genetic diversity of viral gastroenteritis viruses in children younger than 5 years of age in Guatemala, 2014-2015.

J Clin Virol 2019 Mar 9;114:6-11. Epub 2019 Mar 9.

Division of Viral Diseases, US Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address:

Background: Acute diarrhea is an important cause of morbidity and mortality in children and is associated with approximately 500,000 deaths/year globally. Rotavirus and norovirus are leading causes of acute diarrhea accounting for more than half of this burden.

Objective/study Design: To determine the prevalence and genotype distribution of acute diarrhea caused by rotavirus, norovirus, sapovirus and astrovirus among children <5 years of age at two departments in Guatemala from January 2014 to December 2015, we tested 471 stool specimens (202 samples from hospitalized children and 269 samples from children in ambulatory clinics) by real-time reverse transcription-PCR and genotyped positive samples. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.03.006DOI Listing
March 2019
2 Reads

Immune and cytokine/chemokine responses of PBMCs in rotavirus-infected rhesus infants and their significance in viral pathogenesis.

J Med Virol 2019 Mar 15. Epub 2019 Mar 15.

Institute of Medical Biology, Chinese Academy of Medical Science & Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on severe Infectious Disease, Kunming, China.

The rotavirus (RV) is the most important causative agent of severe gastroenteritis in infants and children aged less than 5 years worldwide. However, the response and the roles of peripheral blood mononuclear cell (PBMC) in RV clearance have yet to be fully elucidated. In this study, we established the neonatal rhesus monkey model of RV infection with histopathological changes in the small intestine. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jmv.25460
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http://dx.doi.org/10.1002/jmv.25460DOI Listing
March 2019
14 Reads

Monitoring telehealth vomiting calls as a potential public health early warning system for seasonal norovirus activity in Ontario, Canada.

Epidemiol Infect 2019 Jan;147:e112

Department of Population Medicine,University of Guelph,Guelph, Ontario,Canada.

Norovirus is a predominant cause of infectious gastroenteritis in countries worldwide [1-5]. It accounts for approximately 50% of acute gastroenteritis (AGE) and >90% of viral gastroenteritis outbreaks [6, 7]. The incubation period ranges between 10 and 48 h and illness duration is generally 1-3 days with self-limiting symptoms; however, this duration is often longer (e. Read More

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http://dx.doi.org/10.1017/S0950268818003357DOI Listing
January 2019
1 Read

Glycolysis Is an Intrinsic Factor for Optimal Replication of a Norovirus.

MBio 2019 03 12;10(2). Epub 2019 Mar 12.

Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA

The metabolic pathways of central carbon metabolism, glycolysis and oxidative phosphorylation (OXPHOS), are important host factors that determine the outcome of viral infections and can be manipulated by some viruses to favor infection. However, mechanisms of metabolic modulation and their effects on viral replication vary widely. Herein, we present the first metabolomics and energetic profiling of norovirus-infected cells, which revealed increases in glycolysis, OXPHOS, and the pentose phosphate pathway (PPP) during murine norovirus (MNV) infection. Read More

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http://dx.doi.org/10.1128/mBio.02175-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414699PMC
March 2019
3 Reads

The Dynamic Capsid Structures of the Noroviruses.

Viruses 2019 Mar 8;11(3). Epub 2019 Mar 8.

Department of Biochemistry and Molecular Biology, University of Texas Medical Branch at Galveston, 301 University Boulevard, Galveston, TX 77555-0645, USA.

Noroviruses are responsible for almost a fifth of all cases of gastroenteritis worldwide. New strains evolve every 2⁻4 years by escaping herd immunity and cause worldwide epidemics. In the US alone, noroviruses are responsible for ~20 million cases and more than 70,000 hospitalizations of infected children, annually. Read More

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http://dx.doi.org/10.3390/v11030235DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466125PMC
March 2019
1 Read

Genetic Susceptibility to Human Norovirus Infection: An Update.

Viruses 2019 Mar 6;11(3). Epub 2019 Mar 6.

Division of Molecular Virology, Department of Clinical and Experimental Medicine, Linköping University, 58185 Linköping, Sweden.

Noroviruses are the most common etiological agent of acute gastroenteritis worldwide. Despite their high infectivity, a subpopulation of individuals is resistant to infection and disease. This susceptibility is norovirus genotype-dependent and is largely mediated by the presence or absence of human histo-blood group antigens (HBGAs) on gut epithelial surfaces. Read More

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http://dx.doi.org/10.3390/v11030226DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466115PMC
March 2019
10 Reads

Characterization of a hepatitis C virus genotype 1 divergent isolate from an HIV-1 coinfected individual in Germany assigned to a new subtype 1o.

Virol J 2019 03 4;16(1):28. Epub 2019 Mar 4.

Division of Viral Gastroenteritis, Hepatitis Pathogens and Enteroviruses, Department of Infectious Diseases, Robert Koch Institute, 13353, Berlin, Germany.

Background: HCV exhibits a high genetic diversity and is classified into 7 genotypes which are further divided into 86 confirmed subtypes. However, there are multiple isolates with unassigned subtypes. We aimed to amplify and characterize the full-length genome sequence of an HCV genotype 1 (HCV-1) divergent isolate (DE/17-0414) in Germany. Read More

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http://dx.doi.org/10.1186/s12985-019-1135-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399946PMC
March 2019
3 Reads

Children Attending Day Care Centers are a Year-round Reservoir of Gastrointestinal Viruses.

Sci Rep 2019 Mar 1;9(1):3286. Epub 2019 Mar 1.

Statens Serum Institut, Department of Bacteria, Parasites and Fungi, Copenhagen, Denmark.

Viral gastroenteritis causes high morbidity worldwide. In this study, stool samples from 179 children aged 0-6 years attending Danish day care centers were investigated for gastrointestinal viruses. Each child was observed for one year with submission of samples and questionnaires every two months. Read More

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http://dx.doi.org/10.1038/s41598-019-40077-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6397223PMC
March 2019
1 Read

Genomics Analyses of GIV and GVI Noroviruses Reveal the Distinct Clustering of Human and Animal Viruses.

Viruses 2019 Mar 1;11(3). Epub 2019 Mar 1.

Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD 20993, USA.

Noroviruses are highly diverse viruses that are the major viral cause of acute gastroenteritis in humans. Although these viruses can infect multiple mammalian species, their potential for zoonosis is not well understood, especially within Genogroup IV (GIV), which contains viruses that infect humans, canines, and felines. The study of GIV viruses has been, in part, hindered by the limited number of complete genomes. Read More

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http://dx.doi.org/10.3390/v11030204DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466045PMC
March 2019
1 Read
3.279 Impact Factor

The coordinating role of the human norovirus minor capsid protein VP2 is essential to functional change and nuclear localization of the major capsid protein VP1.

Arch Virol 2019 Apr 27;164(4):1173-1180. Epub 2019 Feb 27.

Department of Clinical Laboratory, Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai, 201508, China.

Global outbreaks of norovirus (NOV) gastroenteritis are associated with the most prevalent genotype, GII.4. Mutations in the protruding domain 2 (P2 domain) of the norovirus major capsid protein (VP1) result in the emergence of various NOV variants, however, it is unclear whether the minor capsid protein (VP2) also affects the generation of VP1 variants. Read More

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http://dx.doi.org/10.1007/s00705-019-04192-2DOI Listing
April 2019
2 Reads

Molecular Epidemiology of Human Astrovirus in Stool Samples From Patients With Acute Gastroenteritis in Korea, 2013-2017.

Ann Lab Med 2019 Jul;39(4):367-372

Department of Laboratory Medicine, Hallym Univerisity College of Medicine, Chuncheon, Korea.

Background: Human astroviruses (HAstVs) are one of the major causes of acute gastroenteritis. Classic HAstVs can be classified into eight genotypes. We investigated the positive rate of HAstVs and the distribution of HAstV genotypes in strains isolated from patients with acute gastroenteritis in Hwaseong, Korea, in 2013-2017. Read More

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http://dx.doi.org/10.3343/alm.2019.39.4.367DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400717PMC
July 2019
1 Read

Effects of the rotavirus vaccine program across age groups in the United States: analysis of national claims data, 2001-2016.

BMC Infect Dis 2019 Feb 22;19(1):186. Epub 2019 Feb 22.

Department of Epidemiology, Rollins School of Public Health, Emory University, 1518 Clifton Road NE, Atlanta, GA, 30322, USA.

Background: The direct effectiveness of infant rotavirus vaccination implemented in 2006 in the United States has been evaluated extensively, however, understanding of population-level vaccine effectiveness (VE) is still incomplete.

Methods: We analyzed time series data on rotavirus gastroenteritis (RVGE) and all-cause acute gastroenteritis (AGE) hospitalization rates in the United States from the MarketScan® Research Databases for July 2001-June 2016. Individuals were grouped into ages 0-4, 5-9, 10-14, 15-24, 25-44, and 45-64 years. Read More

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http://dx.doi.org/10.1186/s12879-019-3816-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387516PMC
February 2019
3 Reads

[Norovirus nosocomial outbreak : an hospital experience].

Rev Med Liege 2019 Feb;74(2):86-89

Service de Microbiologie Clinique, CHU de Liège, Liège, Belgique.

Human noroviruses (NoV) are the main pathogenic agents worldwide responsible for viral sporadic and epidemic gastroenteritis worldwide. A gastroenteritis outbreak broke out in patients hospitalized in several wards located in two different floors of a hospital in Liege, Belgium. The objective was to determine whether a same NoV strain would be involved in the two different floors, and to explore how this outbreak would have spread from a floor to the other. Read More

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February 2019
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GII.4 Human Norovirus: Surveying the Antigenic Landscape.

Viruses 2019 Feb 20;11(2). Epub 2019 Feb 20.

Department of Epidemiology, Gillings School of Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

Human norovirus is the leading cause of viral acute onset gastroenteritis disease burden, with 685 million infections reported annually. Vulnerable populations, such as children under the age of 5 years, the immunocompromised, and the elderly show a need for inducible immunity, as symptomatic dehydration and malnutrition can be lethal. Extensive antigenic diversity between genotypes and within the GII. Read More

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http://dx.doi.org/10.3390/v11020177DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410000PMC
February 2019
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Targeting the Viral Polymerase of Diarrhea-Causing Viruses as a Strategy to Develop a Single Broad-Spectrum Antiviral Therapy.

Viruses 2019 Feb 20;11(2). Epub 2019 Feb 20.

KU Leuven-Laboratory of Virology and Chemotherapy, Department of Microbiology and Immunology, Rega Institute for Medical Research, University of Leuven, Leuven 3000, Belgium.

Viral gastroenteritis is an important cause of morbidity and mortality worldwide, being particularly severe for children under the age of five. The most common viral agents of gastroenteritis are noroviruses, rotaviruses, sapoviruses, astroviruses and adenoviruses, however, no specific antiviral treatment exists today against any of these pathogens. We here discuss the feasibility of developing a broad-spectrum antiviral treatment against these diarrhea-causing viruses. Read More

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http://dx.doi.org/10.3390/v11020173DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409847PMC
February 2019
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Phenotyping of Lewis and secretor HBGA from saliva and detection of new FUT2 gene SNPs from young children from the Amazon presenting acute gastroenteritis and respiratory infection.

Infect Genet Evol 2019 Jun 18;70:61-66. Epub 2019 Feb 18.

Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation (FIOCRUZ), Avenida Brasil, 4365-Manguinhos, Rio de Janeiro, RJ, Brazil.

The Histo-blood group antigens (HBGA) are host genetic factors associated with susceptibility to rotavirus (RV) and human norovirus (HuNoV), the major etiological agents of viral acute gastroenteritis (AGE) worldwide. The FUT2 gene expressing the alpha-1, 2-L- fucosyltransferase enzyme is important for gut HBGA expression, and also provides a composition of the phenotypic profile achieved through mutations occurring in populations with different evolutionary histories; as such, it can be considered a genetic population marker. In this study, Lewis and secretor HBGA phenotyping was performed using 352 saliva samples collected from children between three months and five years old born in the Amazon (Brazil, Venezuela and English Guyana) presenting AGE or acute respiratory infection (ARI), the latter considered as control samples. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15671348183091
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http://dx.doi.org/10.1016/j.meegid.2019.02.011DOI Listing
June 2019
15 Reads

[Molecular epidemiology of norovirus in children with acute gastroenteritis in Tianjin, China].

Zhongguo Dang Dai Er Ke Za Zhi 2019 Feb;21(2):139-143

Pediatric Research Institute, Tianjin Children's Hospital, Tianjin 300134, China.

Objective: To investigate the molecular epidemiological characteristics of norovirus (NoV) among children with acute gastroenteritis in Tianjin in 2017.

Methods: A total of 758 stool specimens were collected from the children with acute gastroenteritis possibly caused by viral infection in Tianjin Children's Hospital between January and December, 2017. Quantitative real-time RT-PCR was used for primary screening of NoV, and conventional RT-PCR was used for gene amplification, sequencing and genotype identification of the VP1 region of capsid protein in positive specimens. Read More

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February 2019
7 Reads

Changes in norovirus genotype diversity in gastroenteritis outbreaks in Alberta, Canada: 2012-2018.

BMC Infect Dis 2019 Feb 19;19(1):177. Epub 2019 Feb 19.

Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada.

Background: The emergence of norovirus genotype GII.4 variants has been associated with gastroenteritis pandemics worldwide, prompting molecular surveillance for early detection of novel strains. In this study, we aimed to analyze the outbreak activity of norovirus and characterize the norovirus strains circulating in Alberta between July 2012 and February 2018. Read More

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http://dx.doi.org/10.1186/s12879-019-3792-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381812PMC
February 2019
1 Read

Profiling of rotavirus 3'UTR-binding proteins reveals the ATP synthase subunit ATP5B as a host factor that supports late-stage virus replication.

J Biol Chem 2019 Apr 15;294(15):5993-6006. Epub 2019 Feb 15.

From the Department of Medicine, Division of Gastroenterology and Hepatology, and

Genome replication and virion assembly of segmented RNA viruses are highly coordinated events, tightly regulated by sequence and structural elements in the UTRs of viral RNA. This process is poorly defined and likely requires the participation of host proteins in concert with viral proteins. In this study, we employed a proteomics-based approach, named RNA-protein interaction detection (RaPID), to comprehensively screen for host proteins that bind to a conserved motif within the rotavirus (RV) 3' terminus. Read More

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http://dx.doi.org/10.1074/jbc.RA118.006004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6463704PMC
April 2019
2 Reads

Reduction of hospitalizations with diarrhea among children aged 0-5 years in Nouakchott, Mauritania, following the introduction of rotavirus vaccine.

Vaccine 2019 Mar 11;37(11):1407-1411. Epub 2019 Feb 11.

Unity of Gastroenteritis, Department of Virology, National Institute of Hygiene, Rabat, Morocco.

Introduction: Rotavirus vaccine was introduced in Mauritania in December 2014. We investigated hospitalizations with diarrhea during pre and post-vaccination periods among children aged 0-5 years in Nouakchott, the capital of Mauritania.

Methods: We conducted a retrospective review of hospital admission registries from November 1st 2012 through October 31th 2017 at all referral hospitals in Nouakchott. Read More

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http://dx.doi.org/10.1016/j.vaccine.2019.01.078DOI Listing
March 2019
4 Reads

Intrahost viral evolution during chronic sapovirus infections.

J Clin Virol 2019 Apr 7;113:1-7. Epub 2019 Feb 7.

Institute of Virology, Leipzig University, Leipzig, Germany Johannisallee 30, 04103, Leipzig, Germany.

Background: Sapovirus is a common cause of self-limiting diarrhea. In immunocompromised individuals chronic infections occur, but are incompletely investigated.

Objectives: To investigate viral evolution in immunocompromised hosts during chronic sapovirus infection. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13866532193002
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http://dx.doi.org/10.1016/j.jcv.2019.02.001DOI Listing
April 2019
8 Reads

G1P[8] Rotavirus in children with severe diarrhea in the post-vaccine introduction era in Brazil: Evidence of reassortments and structural modifications of the antigenic VP7 and VP4 regions.

Infect Genet Evol 2019 Apr 11;69:255-266. Epub 2019 Feb 11.

Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Brazil. Electronic address:

Worldwide rotaviruses A (RVA) are responsible for approximately 215,000 deaths annually among children aged <5 years. RVA G1P[8] remains associated with >50% of gastroenteritis cases in this age group. The aim of this study was to assess the genetic variability of G1P[8] strains detected in children with severe diarrhea in Belém, Pará, Brazil, during the post-rotavirus vaccine introduction era. Read More

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http://dx.doi.org/10.1016/j.meegid.2019.02.009DOI Listing

Comparative Evaluation of Seegene Allplex Gastrointestinal, Luminex xTAG Gastrointestinal Pathogen Panel, and BD MAX Enteric Assays for Detection of Gastrointestinal Pathogens in Clinical Stool Specimens.

Arch Pathol Lab Med 2019 Feb 14. Epub 2019 Feb 14.

From the Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea (Drs Yoo, J. Park, H. K. Lee, and Y.-J. Park); the Department of Laboratory Medicine, Seoul St Mary's Hospital, The Catholic University of Korea, Seoul, Korea (Mss Yu and K. G. Park and Mr G. D. Lee); and the Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge (Ms Oak).

Context.—: Infectious gastroenteritis is caused by various pathogens, including bacteria, viruses, and parasites.

Objective. Read More

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http://dx.doi.org/10.5858/arpa.2018-0002-OADOI Listing
February 2019
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Human Sapovirus among Outpatients with Acute Gastroenteritis in Spain: A One-Year Study.

Viruses 2019 Feb 8;11(2). Epub 2019 Feb 8.

Department of Microbiology and Parasitology, CIBUS-Faculty of Biology, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.

Viral agents of human gastroenteritis affect people of all ages across the globe. As a mainly self-limiting disease, it is difficult to evaluate the real prevalence of etiological agents circulating in each region. Many of the analyzed outbreaks are caused by viruses of the family Caliciviridae, especially the genus (NoV). Read More

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http://dx.doi.org/10.3390/v11020144DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409837PMC
February 2019
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Prevalence, levels and seasonal variations of human enteric viruses in six major rivers in Alberta, Canada.

Water Res 2019 Apr 1;153:349-356. Epub 2019 Feb 1.

Department of Paediatrics, University of Alberta, 1405-87 Ave, Edmonton, AB, T6G 1C9, Canada.

The prevalence and seasonal variation of 7 viruses in 6 major rivers in Alberta were assessed using a combination of qPCR, cell culture and integrated cell culture with qPCR (ICC-qPCR). Water samples were collected monthly from rivers at different sites upstream and downstream of major urban centers. Seven viruses including rotavirus, adenovirus, astrovirus, norovirus, sapovirus, JC virus and enterovirus, were detected in at least one of the water samples at each site using qPCR. Read More

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http://dx.doi.org/10.1016/j.watres.2019.01.034DOI Listing
April 2019
3 Reads

Impact of long-term storage of clinical samples collected from 1996 to 2017 on RT-PCR detection of norovirus.

J Virol Methods 2019 May 6;267:35-41. Epub 2019 Feb 6.

Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Atlanta, GA 30333, USA. Electronic address:

Noroviruses are recognized as the leading cause of acute gastroenteritis globally. With improved molecular diagnostics developed over the last two decades, archived clinical specimens are increasingly used to investigate the historic prevalence and molecular epidemiology of human norovirus. Yet the impact of long-term storage on viral integrity in clinical specimens has not been evaluated. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.02.001DOI Listing
May 2019
1 Read