1,605 results match your criteria Future Microbiology[Journal]


Posaconazole oral suspension as salvage therapy for invasive fungal disease in patients with hematological diseases.

Future Microbiol 2019 Mar 15. Epub 2019 Mar 15.

Hematopoietic Stem Cell Transplantation Center, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China.

Aim: Posaconazole (POS) is an extended-spectrum triazole approved for treatment of refractory invasive fungal disease. Outcome analysis of POS salvage therapy is of great clinical significance.

Methods: We retrospectively reviewed data for 113 patients who received POS oral suspension as salvage therapy between December 2013 and May 2018. Read More

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http://dx.doi.org/10.2217/fmb-2018-0344DOI Listing

Merkel cell polyomavirus DNA sequences in the blood of healthy population of Pakistan.

Future Microbiol 2019 Mar 13. Epub 2019 Mar 13.

School of Biological Sciences, University of the Punjab, Lahore, Pakistan.

Aim: This study aimed at detecting and quantifying Merkel cell polyomavirus (MCPyV) viral loads in the peripheral blood of healthy Pakistani individuals.

Patients & Methods: A total of 221 whole blood samples obtained from healthy individuals were examined by qPCR.

Results & Conclusion: MCPyV was detected in the peripheral blood of 31. Read More

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http://dx.doi.org/10.2217/fmb-2018-0314DOI Listing

Virological surveillance of human respiratory syncytial virus A and B at a tertiary hospital in Catalonia (Spain) during five consecutive seasons (2013-2018).

Future Microbiol 2019 Mar 12. Epub 2019 Mar 12.

Respiratory Virus Unit, Microbiology Department, Hospital Universitari Vall d'Hebron, Vall d'Hebron Research Institute, Universitat Autònoma de Barcelona, Barcelona, Spain.

Aim: Human respiratory syncytial virus (HRSV) is the main cause of respiratory tract infections among infants.

Materials & Methods: In the present study, the molecular epidemiology of HRSV detected from 2013 to 2017 has been described.

Results: A 10% of collected samples were laboratory confirmed for HRSV. Read More

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https://www.futuremedicine.com/doi/10.2217/fmb-2018-0261
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http://dx.doi.org/10.2217/fmb-2018-0261DOI Listing
March 2019
1 Read

First fungemia case due to environmental yeast Wickerhamomyces myanmarensis: detection by multiplex qPCR and antifungal susceptibility.

Future Microbiol 2019 Mar 12. Epub 2019 Mar 12.

Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands.

Aim: Presenting the first clinical case of Wickerhamomyces myanmarensis.

Patients & Methods: Yeast cells were isolated from blood and central venous catheter of a 5.5-year-old male subject. Read More

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http://dx.doi.org/10.2217/fmb-2018-0253DOI Listing

Challenges in Vibrio parahaemolyticus infections caused by the pandemic clone.

Future Microbiol 2019 Mar 11. Epub 2019 Mar 11.

National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Beijing, People's Republic of China.

Vibrio Parahaemolyticus infections caused by the pandemic clone have become a global public health issue. The pandemic clone includes over ten sequence types and 49 serotypes. Several markers such as toxRS/new, orf8 and genomic islands were considered specific for pandemic strains, but subsequent studies later confirmed a lack of specificity. Read More

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https://www.futuremedicine.com/doi/10.2217/fmb-2018-0308
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http://dx.doi.org/10.2217/fmb-2018-0308DOI Listing
March 2019
5 Reads

Asymmetric cell division during Bacillus subtilis sporulation.

Future Microbiol 2019 Mar 11. Epub 2019 Mar 11.

Department of Microbial Genetics, Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia.

Bacillus subtilis is a rod-shaped bacterium which divides precisely at mid-cell during vegetative growth. Unlike Escherichia coli, another model organism used for studying cell division, B. subtilis can also divide asymmetrically during sporulation, the simplest cell differentiation process. Read More

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http://dx.doi.org/10.2217/fmb-2018-0338DOI Listing

Tropheryma whipplei intestinal colonization in Italian and migrant population: a retrospective observational study.

Future Microbiol 2019 Mar 11. Epub 2019 Mar 11.

Centre for Tropical Diseases, IRCCS Sacro Cuore - Don Calabria Hospital, Via Sempreboni 5, 37024 Negrar, Italy.

Aim:  To obtain the first molecular epidemiological survey of T. whipplei intestinal colonization in Italy. Materials & methods: Retrospective, observational study to assess the prevalence of Tropheryma whipplei, the causative agent of Whipple's disease, in stool samples (real-time PCR) of patients attending the Centre for Tropical Diseases (Italy) and risk factors associated. Read More

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http://dx.doi.org/10.2217/fmb-2018-0347DOI Listing

Mucoadhesive S-protected thiolated cyclodextrin-iodine complexes: A promising strategy to prolong mucosal residence time of iodine.

Future Microbiol 2019 Mar 11. Epub 2019 Mar 11.

Department of Pharmaceutical Technology, Center for Chemistry & Biomedicine, Institute of Pharmacy, University of Innsbruck, Innrain 80/82, Innsbruck 6020, Austria.

Aim: The purpose of this study was to develop S-protected thiolated α-cyclodextrin-iodine complexes providing prolonged mucosal residence time and sustained release of the antimicrobial agent.

Materials & Methods: First of all, L-cysteine was conjugated with 2-mercaptonicotinic acid to generate cysteine-2-mercaptonicotinic acid (Cys-MNA). Subsequently, α-CD was oxidized with NaIO and Cys-MNA was bound to the resulting aldehyde groups via reductive amination. Read More

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http://dx.doi.org/10.2217/fmb-2018-0288DOI Listing

Identification of potential tobramycin-resistant mutagenesis of Escherichia coli strains after spaceflight.

Future Microbiol 2019 Mar 11. Epub 2019 Mar 11.

Nanlou Respiratory Diseases Department, Chinese PLA General Hospital, Beijing, China.

Aim: This study aimed to explore potential tobramycin-resistant mutagenesis of Escherichia coli strains after spaceflight.

Materials & Methods: A spaceflight-induced mutagenesis of multidrug resistant E. coli strain (T1_13) on the outer space for 64 days (ST5), and a ground laboratory with the same conditions (GT5) were conducted. Read More

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http://dx.doi.org/10.2217/fmb-2018-0273DOI Listing

Analysis of some immunogenic properties of the recombinant Sporothrix schenckii Gp70 expressed in Escherichia coli.

Future Microbiol 2019 Mar 11. Epub 2019 Mar 11.

Departamento de Biología, División de Ciencias Naturales y Exactas, Campus Guanajuato, Universidad de Guanajuato, Noria Alta s/n, col. Noria Alta, C.P. 36050, Guanajuato, Gto., México.

Aim: Sporothrix schenckii is the causative agent of sporotrichosis. A 70-kDa glycoprotein, Gp70, is a candidate for the development of prophylactic alternatives to control the disease, and its gene (GP70) is predicted to encode for a protein of 43 kDa, contrasting with the molecular weight of the native protein.

Materials & Methods: The GP70 was expressed in bacteria, the recombinant protein purified, used in immunoassays and injected to Galleria mellonella. Read More

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http://dx.doi.org/10.2217/fmb-2018-0295DOI Listing

ZTI-01 (fosfomycin for injection) in the treatment of hospitalized patients with complicated urinary tract infections.

Future Microbiol 2019 Mar 11. Epub 2019 Mar 11.

Department of Pharmacy Practice, College of Pharmacy, University of Illinois at Chicago, Chicago, IL, 60612, USA.

Fosfomycin is a bactericidal antibiotic available since the 1970s whose intravenous formulation has been available in many countries outside the USA. Given the rise in drug resistant bacteria, its introduction into the US market has become a necessity for addressing these organisms. This review provides an overview of the microbiology, clinical pharmacology and initial clinical experiences of the intravenous fosfomycin product (ZTI-01) that is undergoing clinical development in the USA for the treatment of complicated urinary tract infections and acute pyelonephritis. Read More

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http://dx.doi.org/10.2217/fmb-2018-0303DOI Listing

Could targeting neighboring bacterial populations help combat bacterial vaginosis?

Authors:
Nuno Cerca

Future Microbiol 2019 Mar 11. Epub 2019 Mar 11.

Centre of Biological Engineering (CEB), Laboratory of Research in Biofilms Rosário Oliveira, University of Minho, Campus de Gualtar, Braga, Portugal.

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http://dx.doi.org/10.2217/fmb-2019-0045DOI Listing

Septic patients in the intensive care unit present different nasal microbiotas.

Future Microbiol 2019 Feb 26. Epub 2019 Feb 26.

Department of Environmental Health, School of Public Health, Southern Medical University, Guangzhou, PR China.

Aim: The primary objective of this study was to evaluate correlations among mortality, intensive care unit (ICU) length of stay and airway microbiotas in septic patients.

Materials & Methods: A deep-sequencing analysis of the 16S rRNA gene V4 region was performed.

Results: The nasal microbiota in septic patients was dominated by three nasal bacterial types (Corynebacterium, Staphylococcus and Acinetobacter). Read More

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http://dx.doi.org/10.2217/fmb-2018-0349DOI Listing
February 2019
2 Reads

The gut microbiome and the potentials of probiotics: an interview with Simon Gaisford.

Authors:
Simon Gaisford

Future Microbiol 2019 Feb 13. Epub 2019 Feb 13.

Research Department of Pharmaceutics, School of Pharmacy, University College London, London, UK.

In this exclusive interview, Simon Gaisford discusses his current research into gut dysbiosis and its possible relevance to disease states, as well as the efficacy of probiotic supplementation and its possible use as treatment. This interview was conducted by Ellen Colvin, Commissioning Editor of Future Microbiology. Professor Simon Gaisford is the Head of Department at UCL School of Pharmacy, University College London. Read More

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http://dx.doi.org/10.2217/fmb-2019-0016DOI Listing
February 2019
1 Read

Characterization of membrane vesicles released by Mycobacterium avium in response to environment mimicking the macrophage phagosome.

Future Microbiol 2019 Feb 13. Epub 2019 Feb 13.

Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA.

Aim: To investigate the formation of Mycobacterium avium membrane vesicles (MVs) within macrophage phagosomes.

Materials & Methods: A phagosome model was utilized to characterize proteomics and lipidomics of MVs. A click chemistry-based enrichment assay was employed to examine the presence of MV proteins in the cytosol of host cells. Read More

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http://dx.doi.org/10.2217/fmb-2018-0249DOI Listing
February 2019
1 Read

Eugenol and derivatives activity against Mycobacterium tuberculosis, nontuberculous mycobacteria and other bacteria.

Future Microbiol 2019 Feb 13. Epub 2019 Feb 13.

Postgraduate Program in Bioscience & Physiopathology, Department of Clinical Analysis & Biomedicine, State University of Maringa, Parana, Brazil.

Aim:  To evaluate (i) the in vitro activity of eugenol (EUG) and three derivatives against Mycobacterium tuberculosis (Mtb), nontuberculous mycobacteria (NTM) and other bacteria, (ii) the EUG and antituberculosis drugs combinatory effect and (iii) the EUG and its derivatives cytotoxicity.

Materials & Methods:  Minimum inhibitory concentration of the compounds were determined by resazurin microtiter or broth microdilution assay and the drug interaction between EUG and antituberculosis drugs by resazurin drug combination microtiter. The cytotoxicity was carried out in macrophages, HeLa and VERO cells. Read More

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http://dx.doi.org/10.2217/fmb-2018-0333DOI Listing
February 2019
1 Read

Tape mount immunostaining: a versatile method for immunofluorescence analysis of fungi.

Future Microbiol 2019 Feb 13. Epub 2019 Feb 13.

Department of Veterinary Sciences, Institute for Infectious Diseases & Zoonoses, Bacteriology & Mycology, LMU Munich, Germany.

Aim: Immunofluorescence microscopy is a powerful technique to detect surface antigens and study their distribution. Analysis of fungi is often hampered by their weak adherence to glass. We therefore established a novel immunofluorescence staining method to overcome this problem. Read More

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http://dx.doi.org/10.2217/fmb-2018-0283DOI Listing
February 2019

Pathogen-associated acute encephalitis syndrome: therapeutics and management.

Future Microbiol 2019 Feb 13. Epub 2019 Feb 13.

Center for Advanced Research (CFAR) - Stem Cell/Cell Culture Unit, Faculty of Medicine, King George's Medical University (KGMU), Lucknow 226003, India.

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http://dx.doi.org/10.2217/fmb-2018-0334DOI Listing
February 2019
4 Reads

Welcome to the 14 volume of Future Microbiology.

Authors:
Ellen Colvin

Future Microbiol 2019 01;14:1-3

Future Science Group, Unitec House 2 Albert Place, Albert Pl, London N3 1QB, UK.

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http://dx.doi.org/10.2217/fmb-2018-0331DOI Listing
January 2019

Reporting elevated vancomycin minimum inhibitory concentration in methicillin-resistant Staphylococcus aureus: consensus by an International Working Group.

Future Microbiol 2019 Feb 6. Epub 2019 Feb 6.

Department of Clinical Microbiology, Royal University Hospital, Saskatchewan, Canada.

Methicillin-resistant Staphylococcus aureus (MRSA) remains an important cause of serious infection, for which vancomycin is often recommended as the first-choice antibiotic treatment. Appropriate vancomycin prescribing requires accurate measurement of minimum inhibitory concentrations (MICs) to avoid treatment failure, and yet determination can be challenging due to methodological difficulties associated with susceptibility testing. An International Working Group of infectious disease specialists and clinical/medical microbiologists reached a consensus that empirical MRSA infection therapies should be chosen regardless of the suspected origin of the infecting strain (e. Read More

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http://dx.doi.org/10.2217/fmb-2018-0346DOI Listing
February 2019
1 Read

Genetic diversity and evolutionary features of type VI secretion systems in Salmonella.

Future Microbiol 2019 Jan 23;14:139-154. Epub 2019 Jan 23.

Systemomics Center, College of Pharmacy & Genomics Research Center, Harbin Medical University, Harbin, PR China.

Aim: Type VI secretion systems (T6SS) play key roles in bacterial pathogenesis, but their evolutionary features remain largely unclear. In this study, we conducted systematic comparisons among the documented T6SSs in Salmonella and determined their structural diversity, phylogenetic distribution and lineage-specific properties.

Materials & Methods: We screened 295 Salmonella genomes for 13 T6SS core components by hidden Markov models and identified 363 T6SS clusters covering types i1, i2, i3 and i4a. Read More

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http://dx.doi.org/10.2217/fmb-2018-0260DOI Listing
January 2019
3 Reads

Effects of tamoxifen on periodontal disease and Candida albicans of patients with breast cancer and other pathologies.

Future Microbiol 2019 Jan 23;14:129-137. Epub 2019 Jan 23.

Cátedra de Farmacología, Facultad de Odontología, Universidad de Buenos Aires, MT de Alvear 2142, Buenos Aires, Argentina.

Candida albicans is involved in periodontal disease, which is influenced by sex hormones.

Aim: To study the effects of the estrogen antagonist tamoxifen (TAM) on periodontal disease of oncological patients; clinical oral strains of C. albicans. Read More

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http://dx.doi.org/10.2217/fmb-2018-0272DOI Listing
January 2019
3 Reads

Real-time PCR followed by high-resolution melting analysis - a new robust approach to evaluate SCCmec typing of methicillin-resistant Staphylococcus aureus.

Future Microbiol 2019 Jan 22;14:155-164. Epub 2019 Jan 22.

Department of Pathobiology, School of Public Health, and Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran.

Aim: We designed a novel approach based on real-time PCR followed by high-resolution melting (HRM) analysis to determine the Staphylococcal cassette chromosome mec (SCCmec) types of methicillin-resistant Staphylococcus aureus  strains, which we compared against the results of conventional multiplex PCR SCCmec typing.

Methods: Multiplex PCR (for ccr and mec gene complexes) was carried out as conventional method. The HRM analysis was then designed using standard strains of each SCCmec type. Read More

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http://dx.doi.org/10.2217/fmb-2018-0193DOI Listing
January 2019

New 4-methoxy-naphthalene derivatives as promisor antifungal agents for paracoccidioidomycosis treatment.

Future Microbiol 2019 Feb 21;14:235-245. Epub 2019 Jan 21.

Department of Chemistry, State University of Maringá, Maringá, PR, Brazil.

Aim: Novel 4-methoxy-naphthalene derivatives were synthesized based on hits structures in order to evaluate the antifungal activity against Paracoccidioides spp.

Methods: Antifungal activity of compounds was evaluated against P. brasiliensis and most promising compounds 2 and 3 were tested against eight clinically important fungal species. Read More

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http://dx.doi.org/10.2217/fmb-2018-0276DOI Listing
February 2019
3 Reads

Accuracy of rapid test for diagnosis of hepatitis A with different infection rate settings and with predictive modeling.

Future Microbiol 2019 Feb 21;14:247-258. Epub 2019 Jan 21.

Laboratory of Molecular Virology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.

Aim: We evaluated the accuracy of a commercial rapid immunochromatographic test (rapid test [RT]) for hepatitis A (HA) diagnosis and epidemiological studies.

Materials & Methods: The accuracy of a RT was evaluated in laboratory and in field conditions. Predictive modeling estimated the test performance in a hypothetical population. Read More

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http://dx.doi.org/10.2217/fmb-2018-0211DOI Listing
February 2019
2 Reads

Quantitative detection of a cocktail of mycobacterial MPT64 and PstS1 in tuberculosis patients by real-time immuno-PCR.

Future Microbiol 2019 Feb 21;14:223-233. Epub 2019 Jan 21.

Centre for Biotechnology, Maharshi Dayanand University, Rohtak-124001, India.

Aim: There is an urgent need to design a reliable diagnostic test for tuberculosis (TB).

Methods: Real-time immuno-PCR (RT-I-PCR) assay was devised for the quantitative detection of a cocktail of mycobacterial MPT64 (Rv1980c) and PstS1 (Rv0934) in TB patients.

Results: A broad dynamic range of 0. Read More

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http://dx.doi.org/10.2217/fmb-2018-0284DOI Listing
February 2019
4 Reads
4.275 Impact Factor

Utilization of the ComRS system for the rapid markerless deletion of chromosomal genes in Streptococcus suis.

Future Microbiol 2019 Feb 21;14:207-222. Epub 2019 Jan 21.

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.

Aim: To develop a markerless gene deletion strategy in Streptococcus suis to solve the problem that several serotypes against electrotransformation of foreign DNA.

Materials & Methods: Bioinformatics retrieval was performed to identified ComRS systems functioning for natural transformation. A sacB-spc cassette with the upper and lower homologous fragments was amplification by fusion-PCR for spectinomycin-positive and sucrose-negative selection during gene deletion. Read More

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http://dx.doi.org/10.2217/fmb-2018-0279DOI Listing
February 2019
2 Reads

The fecal and mucosal microbiome in acute appendicitis patients: an observational study.

Future Microbiol 2019 Jan 21;14:111-127. Epub 2019 Jan 21.

Department of Infectious Diseases & Immunity, Jessa Hospital, Hasselt, Belgium.

Aim: We aimed to study the mucosal microbiota of the appendix in a prospective appendicitis cohort and to compare the fecal microbiota of patients and controls. We hypothesized that the microbiota may be associated with susceptibility to appendicitis.

Patients & Methods: The fecal microbiota of 99 patients and 106 controls were characterized using 16S-23S intergenic spacer profiling. Read More

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http://dx.doi.org/10.2217/fmb-2018-0203DOI Listing
January 2019
3 Reads

Modulatory effects of verapamil in rifampicin activity against Mycobacterium tuberculosis.

Future Microbiol 2019 Feb 16;14:185-194. Epub 2019 Jan 16.

Department of Biological Sciences, School of Pharmaceutical Sciences, Paulista State University, Araraquara, Sao Paulo, Brazil.

Aim: To evaluate modulatory effect of verapamil (VP) in rifampicin (RIF) activity and its effect in efflux pumps (EPs) transcript levels in Mycobacterium tuberculosis.

Materials & Methods: RIF and VP minimal inhibitory concentration, combinatory effect and detection of mutations were determined in 16 isolates. EPs transcript levels were determined in four isolates by real-time PCR after exposure to drugs. Read More

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https://www.futuremedicine.com/doi/10.2217/fmb-2018-0277
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http://dx.doi.org/10.2217/fmb-2018-0277DOI Listing
February 2019
9 Reads

Plantaricins markedly enhance the effects of traditional antibiotics against Staphylococcus epidermidis.

Future Microbiol 2019 Feb 16;14:195-205. Epub 2019 Jan 16.

Faculty of Medicine & Health, School of Medical Sciences, Örebro University, Örebro, Sweden.

Aim: Bacteriocins are considered as promising alternatives to antibiotics against infections. In this study, the plantaricins (Pln) A, E, F, J and K were investigated for their antimicrobial activity against Staphylococcus epidermidis.

Materials & Methods: The effects on membrane integrity were studied using liposomes and viable bacteria, respectively. Read More

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http://dx.doi.org/10.2217/fmb-2018-0285DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393846PMC
February 2019
1 Read

Letermovir for prophylaxis of cytomegalovirus manifestations in adult allogeneic hematopoietic stem cell transplant recipients.

Future Microbiol 2019 Feb 15;14:175-184. Epub 2019 Jan 15.

Department of Hematology, University Hospitals Leuven, Leuven, Belgium.

Cytomegalovirus (CMV) manifestations remain important complications after allogeneic hematopoietic stem cell transplantation (HSCT), even in the current era. Unfortunately, available anti-CMV agents, mainly viral polymerase inhibitors, have a substantial risk of myelosuppression and nephrotoxicity. Letermovir, a novel anti-CMV drug that targets the viral terminase complex, has recently been approved for the prevention of clinically significant CMV infection in adult CMV seropositive hematopoietic stem cell transplantation recipients. Read More

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https://www.futuremedicine.com/doi/10.2217/fmb-2018-0250
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http://dx.doi.org/10.2217/fmb-2018-0250DOI Listing
February 2019
13 Reads

Temocillin: is this the right momentum for its global use?

Future Microbiol 2019 Jan 15;14:81-83. Epub 2019 Jan 15.

Department of Microbiology, Medical School, University of Athens, Athens, Greece.

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http://dx.doi.org/10.2217/fmb-2018-0316DOI Listing
January 2019
1 Read

Does infection of cerebellar Purkinje neurons with human herpes virus 6A or 6B (HHV-6) increase the risk of developing mood disorders?

Future Microbiol 2019 Jan 15;14:85-88. Epub 2019 Jan 15.

Institute for Virology & Immunobiology, University of Wuerzburg, Wuerzburg, Germany.

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http://dx.doi.org/10.2217/fmb-2018-0307DOI Listing
January 2019
2 Reads

Use of procalcitonin as a tool for antibiotic stewardship.

Future Microbiol 2019 Jan 15;14:77-79. Epub 2019 Jan 15.

Department di Clinica delle Malattie Infettive, Università Cattolica S Cuore, Fondazione Policlinico A Gemelli IRCCS, Roma, Italy.

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http://dx.doi.org/10.2217/fmb-2018-0326DOI Listing
January 2019
1 Read

The impact of infectious disease in war time: a look back at WW1.

Authors:
Hugh Pennington

Future Microbiol 2019 Feb 10;14:165-168. Epub 2019 Jan 10.

Emeritus Professor of Bacteriology, University of Aberdeen, 13 Carlton Place, Aberdeen AB15 4BR, UK.

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http://dx.doi.org/10.2217/fmb-2018-0323DOI Listing
February 2019
2 Reads

Diagnostic stewardship for sepsis: the need for risk stratification to triage patients for fast microbiology workflows.

Future Microbiol 2019 Feb 10;14:169-174. Epub 2019 Jan 10.

Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.

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http://dx.doi.org/10.2217/fmb-2018-0329DOI Listing
February 2019
1 Read

Can we use human odors to diagnose malaria?

Future Microbiol 2019 01 19;14:5-9. Epub 2018 Dec 19.

Department of Environmental Systems Science, ETH Zürich, 8092 Zürich, Switzerland.

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http://dx.doi.org/10.2217/fmb-2018-0312DOI Listing
January 2019
2 Reads

The transcription of pafA, encoding the prokaryotic ubiquitin-like protein ligase, is regulated by PafBC.

Future Microbiol 2019 01 14;14:11-21. Epub 2018 Dec 14.

Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.

Aim: Mycobacterium tuberculosis possesses an intracellular tagging and degradation system, which has emerged as a target for development of anti-tuberculosis agents. In this system, PafA is the ligase that marks proteins for degradation by their covalent modification with a protein modifier. Here, we studied pafA transcriptional regulation, which remained elusive despite its importance for M. Read More

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http://dx.doi.org/10.2217/fmb-2018-0278DOI Listing
January 2019
2 Reads

The GenMark ePlex: another weapon in the syndromic arsenal for infection diagnosis.

Future Microbiol 2018 12 14;13:1697-1708. Epub 2018 Dec 14.

Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, NY 10065 USA.

As one of the most recent additions to the syndromic testing landscape, the ePlex platform by GenMark Diagnostics is a system that combines the manufacturer's signature electrochemical detection technology with updated microfluidics, providing a new option for multiplex testing that is both rapid and requires minimal hands-on steps. In this review, we detail the ePlex platform and its current/future syndromic panels, with a particular focus on the respiratory pathogen panel - the platform's first assay to undergo clinical trials and receive regulatory approval in the USA. By keeping informed of these ever-expanding laboratory options, clinicians and microbiologists can stay positioned at the forefront of infectious disease diagnosis. Read More

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https://www.futuremedicine.com/doi/10.2217/fmb-2018-0258
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http://dx.doi.org/10.2217/fmb-2018-0258DOI Listing
December 2018
10 Reads

Novel multiplex real-time quantitative PCR detecting system approach for direct detection of Candida auris and its relatives in spiked serum samples.

Future Microbiol 2019 01 12;14:33-45. Epub 2018 Dec 12.

Westerdijk Fungal Biodiversity Institute, Utrecht 3584, The Netherlands.

The multidrug-resistant opportunistic yeast species of Candida auris, Candida haemulonii, Candida duobushaemulonii and Candida pseudohaemulonii continue to endanger the healthcare settings around the globe. Due to the lack of a specific qPCR assay for detection of these species from clinical samples, we developed a multiplex qPCR assay. Analytical specificity and sensitivity showed 100% specificity and the sensitivity of up to ten genomes of target species with a high value of reproducibility (R >0. Read More

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https://www.futuremedicine.com/doi/10.2217/fmb-2018-0227
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http://dx.doi.org/10.2217/fmb-2018-0227DOI Listing
January 2019
11 Reads

Quorum quenching: role of nanoparticles as signal jammers in Gram-negative bacteria.

Future Microbiol 2019 01 12;14:61-72. Epub 2018 Dec 12.

Department of Microbiology, Government College University, Faisalabad, Pakistan.

Quorum sensing (QS) is a cell density dependent regulatory process that uses signaling molecules to manage the expression of virulence genes and biofilm formation. The study of QS inhibitors has emerged as one of the most fascinating areas of research to discover novel antimicrobial agents. Compounds that block QS have become candidates as unusual antimicrobial agents, as they are leading players in the regulation of virulence of drug-resistant pathogens. Read More

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https://www.futuremedicine.com/doi/10.2217/fmb-2018-0257
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http://dx.doi.org/10.2217/fmb-2018-0257DOI Listing
January 2019
9 Reads

Fast, economic and simultaneous identification of clinically relevant Gram-negative species with multiplex real-time PCR.

Future Microbiol 2019 01 12;14:23-32. Epub 2018 Dec 12.

Research & Development, Abbott (Alere Technologies GmbH), Jena, Germany.

Aim: A newly designed multiplex real-time PCR (rt-PCR) was validated to detect four clinically relevant Gram-negative bacteria (Escherichia coli, Acinetobacter baumannii, Klebsiella pneumoniae and Pseudomonas aeruginosa).

Materials & Methods: Serial dilutions of genomic DNA were used to determine the limit of detection. Colony PCR was performed with isolates of the four selected species and other species as negative controls. Read More

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http://dx.doi.org/10.2217/fmb-2018-0197DOI Listing
January 2019
4 Reads

Mycobacterium tuberculosis Rv1473 is a novel macrolides ABC Efflux Pump regulated by WhiB7.

Future Microbiol 2019 01 12;14:47-59. Epub 2018 Dec 12.

Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment & Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, PR China.

Aim: To characterize a novel macrolide ATP binding cassette efflux pump encoding gene Rv1473 which might be involved in antibiotic resistance.

Methods: Mycobacterium smegmatis was used as a surrogate model for pathogenic mycobacteria, drug susceptibility assays and ethidium bromide accumulation assay were harnessed to verify drug resistance. The real-time quantitative PCR was used to evaluate the transcription levels of WhiB7 and Ms3140 upon exposure to macrolides. Read More

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http://dx.doi.org/10.2217/fmb-2018-0207DOI Listing
January 2019
4 Reads

Structure and immunomodulatory activity of a recombinant mucus-binding protein of Lactobacillus acidophilus.

Future Microbiol 2018 12 11;13:1731-1743. Epub 2018 Dec 11.

Ningbo Dairy Group, Ningbo, 315211, Zhejiang, PR China.

Aim: The role of mucus-binding protein (MUB) on the adhesion activity and immunomodulatory effect of Lactobacillus acidophilus.

Materials & Methods: The current research mainly focuses on the adhesion and immune function of MUB from L. acidophilus. Read More

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http://dx.doi.org/10.2217/fmb-2018-0222DOI Listing
December 2018
2 Reads

How is fosfomycin resistance developed in Escherichia coli?

Future Microbiol 2018 12 11;13:1693-1696. Epub 2018 Dec 11.

CHU de Caen, Service de Microbiologie, Caen, France.

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http://dx.doi.org/10.2217/fmb-2018-0294DOI Listing
December 2018
3 Reads

The CRISPR-cas system promotes antimicrobial resistance in Campylobacter jejuni.

Future Microbiol 2018 12 11;13:1757-1774. Epub 2018 Dec 11.

China MOA Laboratory for Risk Assessment of Quality & Safety of Livestock & Poultry Products, Huazhong Agricultural University, Wuhan 430070, PR China.

Aim: The purpose of current study is to find out relationship between cas9 gene and antimicrobial resistance in Campylobacter jejuni NCTC11168.

Materials & Methods: The involvement of the cas9 gene in antimicrobial resistance of C. jejuni was determined by assessment of minimum inhibitory concentration, clustered regularly interspaced short palindromic repeats (CRISPR)-cas gene expression in standard strains, in vitro resistance development and transcriptome analysis of a cas9 deletion mutant and wild strains. Read More

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http://dx.doi.org/10.2217/fmb-2018-0234DOI Listing
December 2018
2 Reads
4.275 Impact Factor

Fusarium oxysporum is an onychomycosis etiopathogenic agent.

Future Microbiol 2018 12 3;13:1745-1756. Epub 2018 Dec 3.

Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá (UEM), Avenida Colombo, 5790, Maringá, PR, CEP 87020-900, Brazil.

Aim: To evaluate and characterize the etiopathogenesis of the fusarial onychomycosis in an ex vivo study through fragments of sterile human nail, without the addition of any nutritional source.

Materials & Methods: The infection and invasion of Fusarium oxysporum in the nail were evaluated by scanning electron microscopy (SEM), CFU, matrix, histopathology and Fourier Transform Infrared Spectrometer coupled to an equipment with diamond accessory (FTIR-ATR).

Results: F. Read More

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http://dx.doi.org/10.2217/fmb-2018-0245DOI Listing
December 2018
2 Reads

Antibiotic-nanomedicines: facing the challenge of effective treatment of antibiotic-resistant respiratory tract infections.

Future Microbiol 2018 Nov 30;13:1683-1692. Epub 2018 Nov 30.

Department of Medical Microbiology & Infectious Diseases, Erasmus University Medical Center Rotterdam (Erasmus MC), Rotterdam, The Netherlands.

Respiratory tract infections are one of the most frequent infections worldwide, with an increasing number being associated with (multiple) antibiotic-resistant pathogens. Improved treatment requires the development of new therapeutic strategies, including the possible development of antibiotic-nanomedicines. Antibiotic-nanomedicines comprise antibiotic molecules coupled to nanocarriers via surface adsorption, surface attachment, entrapment or conjugation and can be administered via aerosolization. Read More

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https://www.futuremedicine.com/doi/10.2217/fmb-2018-0194
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http://dx.doi.org/10.2217/fmb-2018-0194DOI Listing
November 2018
10 Reads

Comparative genomics analysis of pTEM-2262, an MDR plasmid from Citrobacter freundii, harboring two unclassified replicons.

Future Microbiol 2018 Nov 30;13:1657-1668. Epub 2018 Nov 30.

College of Life Science, Hebei Normal University, Shijiazhuang, Hebei 050024, PR China.

Aim: To genetically characterize the multidrug-resistance (MDR) plasmid pTEM-2262 that could not be classified into any known incompatibility group from the clinical Citrobacter freundii isolate 2262.

Materials & Methods: The repA or repB deletion mutants of pTEM-2262 were constructed using the scarless Cas9-assisted recombineering system. Comparative genomic analysis of pTEM-2262 and the other four previously sequenced plasmids belonging to the same incompatibility group were performed. Read More

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http://dx.doi.org/10.2217/fmb-2018-0243DOI Listing
November 2018
1 Read

Glecaprevir/pibrentasvir for the treatment of chronic hepatitis C virus infection.

Future Microbiol 2019 Jan 30;14:89-110. Epub 2018 Nov 30.

Department of Pharmacokinetics, AbbVie Inc., North Chicago, IL 60064, USA.

In recent years, management of chronic hepatitis C virus (HCV) infection has been revolutionized by the availability of oral direct-acting antivirals (DAAs), which have significantly better efficacy and safety profiles than interferon-containing regimens. Simple, short-duration DAA therapies will facilitate expansion of HCV treatment to nonspecialist providers, which will be vital to achieve the WHO target of eliminating chronic HCV as a major public health threat by 2030. Coformulated glecaprevir/pibrentasvir is the only 8-week, pan-genotypic, 2-DAA regimen recommended by international guidelines as a first-line regimen in treatment-naive, noncirrhotic HCV genotype 1-6 patients. Read More

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http://dx.doi.org/10.2217/fmb-2018-0233DOI Listing
January 2019
3 Reads