2,226 results match your criteria Fungal Genetics and Biology [Journal]


Chlorophyll fluorescence imaging for monitoring effects of Heterobasidion parviporum small secreted protein induced cell death and in planta defense gene expression.

Fungal Genet Biol 2019 Feb 11. Epub 2019 Feb 11.

Faculty of Agriculture and Forestry, P. O. Box 27, Latokartanonkaari 7, 00014, University of Helsinki, Helsinki, Finland; Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland. Electronic address:

Heterobasidion parviporum Niemelä & Korhonen is a necrotrophic fungal pathogen of Norway spruce (Picea abies). H. parviporum genome encodes numerous necrotrophic small secreted proteins (SSP) which might be important for promoting and sustaining the disease development. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.02.003DOI Listing
February 2019

CRISPR/Cas9-mediated endogenous gene tagging in Fusarium oxysporum.

Fungal Genet Biol 2019 Feb 6. Epub 2019 Feb 6.

Department of Entomology and Plant Pathology, Auburn University, Auburn Alabama 36849, United States. Electronic address:

Fusarium oxysporum is an economically important pathogen that widely exists in the environment and is capable of causing serious problems in crop production and animal/human health. One important step for characterization of a fungal protein with an unknown function is to determine its subcellular localization within the cell. To facilitate the study of important functional regulators or key virulence factors, we developed a CRISPR/Cas9-mediated endogenous gene tagging (EGT) system based on two different strategies, homology-independent targeted integration (HITI) and homology-dependent recombination integration (HDRI). Read More

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http://dx.doi.org/10.1016/j.fgb.2019.02.002DOI Listing
February 2019

Core components of endosomal mRNA transport are evolutionarily conserved in fungi.

Fungal Genet Biol 2019 Feb 6. Epub 2019 Feb 6.

Heinrich Heine University Düsseldorf, Institute for Microbiology, Cluster of Excellence on Plant Sciences, 40204 Düsseldorf, Germany. Electronic address:

Active movement of mRNAs by sophisticated transport machineries determines precise spatiotemporal expression of encoded proteins. A prominent example discovered in fungi is microtubule-dependent transport via endosomes. This mode of transport was thought to be only operational in the basidiomycete Ustilago maydis. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.013DOI Listing
February 2019

Transcriptional profiling and localization of GUL-1, a COT-1 pathway component, in Neurospora crassa.

Fungal Genet Biol 2019 Feb 4;126:1-11. Epub 2019 Feb 4.

Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 761001, Israel.

Impairment of theNeurospora crassaCOT-1 kinase results in defects in hyphal polarity. Some of these effects are partially suppressed by inactivation of gul-1 (encoding an mRNA-binding protein involved in translational regulation). Here, we report on the transcriptional profiling of cot-1 inactivation and demonstrate that gul-1 affects transcript abundance of multiple genes in the COT-1 pathway, including processes such as cell wall remodeling, nitrogen and amino acid metabolism. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.010DOI Listing
February 2019

Complex epigenetic regulation of alkaloid biosynthesis and host interaction by heterochromatin protein I in a fungal endophyte-plant symbiosis.

Fungal Genet Biol 2019 Feb 4;125:71-83. Epub 2019 Feb 4.

School of Fundamental Sciences and the BioProtection Research Centre, Massey University, Private Bag 11 222, Palmerston North 4442, New Zealand. Electronic address:

Epichloë festucae forms mutualistic symbiotic interactions with grasses of the Lolium and Festuca genera. Protection from insect and mammalian herbivory are the best-documented host benefits of these associations. The two main classes of anti-mammalian alkaloids synthesized by E. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.02.001DOI Listing
February 2019
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Genomic comparisons of the laurel wilt pathogen, Raffaelea lauricola, and related tree pathogens highlight an arsenal of pathogenicity related genes.

Fungal Genet Biol 2019 Feb 1;125:84-92. Epub 2019 Feb 1.

Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO 80523, USA. Electronic address:

Raffaelea lauricola is an invasive fungal pathogen and symbiont of the redbay ambrosia beetle (Xyleborus glabratus) that has caused widespread mortality to redbay (Persea borbonia) and other Lauraceae species in the southeastern USA. We compare two genomes of R. lauricola (C2646 and RL570) to seven other related Ophiostomatales species including R. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.012DOI Listing
February 2019

Unique gene Pmhyp controlling melanization of pycnidia in Phoma medicaginis.

Fungal Genet Biol 2019 Jan 30;125:53-59. Epub 2019 Jan 30.

Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, USA.

Phoma medicaginis (syn. Ascochyta medicaginicola Qchen & L. Cai) causes spring black stem and leaf spot of alfalfa and the model legume Medicago truncatula. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.007DOI Listing
January 2019

Comparative Transcriptome Analysis Reveals Regulatory Networks and Key Genes of Microsclerotia Formation in the Cotton Vascular Wilt Pathogen.

Fungal Genet Biol 2019 Jan 30. Epub 2019 Jan 30.

Chongqing Engineering Research Center of Specialty Crop Resources and The College of Life Science, Chongqing Normal University, Chongqing 401331, China. Electronic address:

Verticillium dahliae is a soil-borne, hemibiotrophic phytopathogenic fungus that causes Verticillium wilt in a broad range of economic crops. The microsclerotia (MS), which act as the main host inoculum, can survive long-term in soil resulting in uncontrollable disease. In order to clarify the mechanism of MS formation, we sequenced the whole genome-wide expression profile of V. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.009DOI Listing
January 2019

The interplay between transport and metabolism in fungal itaconic acid production.

Fungal Genet Biol 2019 Jan 28;125:45-52. Epub 2019 Jan 28.

iAMB - Institute of Applied Microbiology, ABBt - Aachen Biology and Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany; Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich, Wilhelm-Johnen-Strasse, 52425 Jülich, Germany. Electronic address:

Besides enzymatic conversions, many eukaryotic metabolic pathways also involve transport proteins that shuttle molecules between subcellular compartments, or into the extracellular space. Fungal itaconate production involves two such transport steps, involving an itaconate transport protein (Itp), and a mitochondrial tricarboxylate transporter (Mtt). The filamentous ascomycete Aspergillus terreus and the unicellular basidiomycete Ustilago maydis both produce itaconate, but do so via very different molecular pathways, and under very different cultivation conditions. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.011DOI Listing
January 2019
1 Read

Mating-type loci of Ustilago esculenta are essential for mating and development.

Fungal Genet Biol 2019 Jan 24;125:60-70. Epub 2019 Jan 24.

Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, China. Electronic address:

Ustilago esculenta is closely related to the smut fungus Ustilago maydis and, in an endophytic-like life in the plant Zizania latifolia, only infects host stems and causes swollen stems to form edible galls called Jiaobai in China. In order to study its different modes of invasion and sites of symptom development from other smut fungi at the molecular level, we first characterized the a and b mating-type loci of U. esculenta. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.008DOI Listing
January 2019
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Expression of the Fusarium graminearum terpenome and involvement of the endoplasmic reticulum-derived toxisome.

Fungal Genet Biol 2019 Jan 18;124:78-87. Epub 2019 Jan 18.

USDA ARS Cereal Disease Laboratory, Saint Paul, MN, USA. Electronic address:

The sesquiterpenoid deoxynivalenol (DON) is an important trichothecene mycotoxin produced by the cereal pathogen Fusarium graminearum. DON is synthesized in specialized subcellular structures called toxisomes. The first step in DON synthesis is catalyzed by the sesquiterpene synthase (STS), Tri5 (trichodiene synthase), resulting in the cyclization of farnesyl diphosphate (FPP) to produce the sesquiterpene trichodiene. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.006DOI Listing
January 2019
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Influence of farming practices on the population genetics of the maize pathogen Cercospora zeina in South Africa.

Fungal Genet Biol 2019 Jan 16;125:36-44. Epub 2019 Jan 16.

Department of Plant and Soil Sciences, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, South Africa. Electronic address:

Gray leaf spot (GLS) is an important foliar disease of maize. This disease, caused by Cercospora zeina, is prevalent in both smallholder and commercial maize farms in South Africa. Notably, smallholder practices are geared towards conservation agriculture, planting diverse maize genotypes within a field and avoiding chemical control. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.005DOI Listing
January 2019

Universal plasmids to facilitate gene deletion and gene tagging in filamentous fungi.

Fungal Genet Biol 2019 Jan 11;125:28-35. Epub 2019 Jan 11.

Faculty of Health Sciences, University of Macau, Avenida da Universidade, Taipa, Macau; Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Avenida da Universidade, Taipa, Macau. Electronic address:

Gene manipulation is an important routine technique and its efficiency is often a rate-limiting step in research. To facilitate gene manipulation in filamentous fungi, we adapted the S. cerevisiae Gene Deletion and Gene Tagging plasmid collections to include additional selectable markers that make the useful resources applicable to other fungi. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.004DOI Listing
January 2019

Phosphoproteomic and transcriptomic analyses reveal multiple functions for Aspergillus nidulans MpkA independent of cell wall stress.

Fungal Genet Biol 2019 Jan 9;125:1-12. Epub 2019 Jan 9.

Department of Chemical, Biochemical, and Environmental Engineering, University of Maryland Baltimore County, Baltimore, MD 21250, United States. Electronic address:

The protein kinase MpkA plays a prominent role in the cell wall integrity signaling (CWIS) pathway, acting as the terminal MAPK activating expression of genes which encode cell wall biosynthetic enzymes and other repair functions. Numerous studies focus on MpkA function during cell wall perturbation. Here, we focus on the role MpkA plays outside of cell wall stress, during steady state growth. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.003DOI Listing
January 2019

The CysHis zinc finger protein Zfp1 regulates sexual reproduction and virulence in Cryptococcus neoformans.

Fungal Genet Biol 2019 Jan 7;124:59-72. Epub 2019 Jan 7.

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory of Microsporidia Infection and Prevention, Southwest University, Chongqing 400715, China. Electronic address:

Cryptococcus neoformans is a ubiquitous yeast pathogen that often infects the human central nervous system (CNS) to cause meningitis in immunocompromised individuals. Although numerous signaling pathways and factors important for fungal sexual reproduction and virulence have been investigated, their precise mechanism of action remains to be further elucidated. In this study, we identified and characterized a novel zinc finger protein Zfp1 that regulates fungal sexual reproduction and virulence in C. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.002DOI Listing
January 2019
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The actin motor MYO-5 effect in the intracellular organization of Neurospora crassa.

Fungal Genet Biol 2019 Jan 4;125:13-27. Epub 2019 Jan 4.

Departamento de Microbiología, Centro de Investigación Científica y de Educación Superior de Ensenada, Ensenada, Baja California, Mexico. Electronic address:

In filamentous fungi, polarized growth is the result of vesicle secretion at the hyphal apex. Motor proteins mediate vesicle transport to target destinations on the plasma membrane via actin and microtubule cytoskeletons. Myosins are motor proteins associated with actin filaments. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.11.008DOI Listing
January 2019
5 Reads

Phosphatidate phosphatase Pah1 has a role in the hyphal growth and virulence of Candida albicans.

Fungal Genet Biol 2019 Jan 4;124:47-58. Epub 2019 Jan 4.

Key Laboratory of Cell Proliferation and Regulation Biology, College of Life Sciences, Beijing Normal University, Beijing, China. Electronic address:

Phosphatidate phosphatases play essential roles in lipid metabolism by converting phosphatidic acid to diacylglycerol. Here, we have investigated the roles of a phosphatidate phosphatase, Pah1, in the fungal pathogen Candida albicans. Deleting PAH1 causes multiple phenotypes, especially severe hyphal defects, increased sensitivity to cell wall stress, and reduced virulence in mice. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.12.010DOI Listing
January 2019
5 Reads

Proteomic characterization of Aspergillus fumigatus isolated from air and surfaces of the International Space Station.

Fungal Genet Biol 2019 Jan 3;124:39-46. Epub 2019 Jan 3.

Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA, USA. Electronic address:

The on-going Microbial Observatory Experiments on the International Space Station (ISS) revealed the presence of various microorganisms that may be affected by the distinct environment of the ISS. The low-nutrient environment combined with enhanced irradiation and microgravity may trigger changes in the molecular suite of microorganisms leading to increased virulence and resistance of microbes. Proteomic characterization of two Aspergillus fumigatus strains, ISSFT-021 and IF1SW-F4, isolated from HEPA filter debris and cupola surface of the ISS, respectively, is presented, along with a comparison to well-studied clinical isolates Af293 and CEA10. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.001DOI Listing
January 2019
6 Reads

Mating type (MAT) locus and possible sexuality of the opportunistic pathogen Exophiala dermatitidis.

Fungal Genet Biol 2019 Jan 3;124:29-38. Epub 2019 Jan 3.

Division of Mycology, Department of Microbiology, Faculty of Medicine, Çukurova University, Adana, Turkey. Electronic address:

Sexual reproduction among the black yeasts is generally limited to environmental saprobic species and is rarely observed among opportunists in humans. To date, a complete sexual cycle has not been observed in Exophiala dermatitidis. In this study, we aimed to gain insight into the reproductive mode of E. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.12.011DOI Listing
January 2019
9 Reads

Nanoscale enrichment of the cytosolic enzyme trichodiene synthase near reorganized endoplasmic reticulum in Fusarium graminearum.

Fungal Genet Biol 2018 Dec 21;124:73-77. Epub 2018 Dec 21.

USDA ARS Cereal Disease Laboratory, 1551 Lindig Street, St. Paul, MN 55108, USA; Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN 55108, USA. Electronic address:

Trichothecene mycotoxin synthesis in the phytopathogen Fusarium graminearum involves primarily endoplasmic reticulum (ER)-localized enzymes of the mevalonate- and trichothecene biosynthetic pathways. Two exceptions are 3-hydroxy-3-methylglutaryl CoA synthase (Hms1) and trichodiene synthase (Tri5), which are known cytosolic enzymes. Using 3D structured illumination microscopy (3D SIM), GFP-tagged Tri5 and Hms1 were tested for preferential localization in the cytosol proximal to the ER. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.12.008DOI Listing
December 2018

Differential roles, crosstalk and response to the Antifungal Protein AfpB in the three Mitogen-Activated Protein Kinases (MAPK) pathways of the citrus postharvest pathogen Penicillium digitatum.

Fungal Genet Biol 2018 Dec 21;124:17-28. Epub 2018 Dec 21.

Department of Food Biotechnology, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Avda Agustín Escardino 7, 46980 Paterna, Valencia, Spain. Electronic address:

Fungi have three mitogen-activated protein kinases (MAPKs): Kss1/Fus3 involved in the invasive growth and virulence of pathogens, Hog1 in response to osmotic stress, and Slt2/Mpk1 in response to cell wall (CW) stress. We conducted comparative analyses of these MAPKs in the phytopathogen Penicillium digitatum and studied their role in the mode of action of the novel self-antifungal protein AfpB. The sensitivity to different stresses of Δhog1 and the reduced growth of Δkss1 coincided with previous reports. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.12.006DOI Listing
December 2018

Nutrient sensing-the key to fungal p53-like transcription factors?

Authors:
Margaret E Katz

Fungal Genet Biol 2018 Dec 21;124:8-16. Epub 2018 Dec 21.

Molecular and Cellular Biology, University of New England, Armidale, NSW 2351, Australia. Electronic address:

The mammalian tumour suppressor protein, p53, plays an important role in cell cycle control, DNA repair and apoptotic cell death. Transcription factors belonging to the "p53-like" superfamily are found exclusively in the Amorphea branch of eukaryotes, which includes animals, fungi and slime molds. Many members of the p53-like superfamily (proteins containing p53, Rel/Dorsal, T-box, STAT, Runt, Ndt80, and the CSL DNA-binding domains) are involved in development. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.12.007DOI Listing
December 2018
1 Read

CRISPR-Cas9 induces point mutation in the mucormycosis fungus Rhizopus delemar.

Fungal Genet Biol 2018 Dec 15;124:1-7. Epub 2018 Dec 15.

Department of Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center, New Orleans, LA, USA; Department of Pediatrics, Louisiana State University Health Sciences Center, New Orleans, LA, USA. Electronic address:

Rhizopus delemar causes devastating mucormycosis in immunodeficient individuals. Despite its medical importance, R. delemar remains understudied largely due to the lack of available genetic markers, the presence of multiple gene copies due to genome duplication, and mitotically unstable transformants resulting from conventional and limited genetic approaches. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.12.002DOI Listing
December 2018
1 Read

The Slt2-MAPK pathway is involved in the mechanism by which target of rapamycin regulates cell wall components in Ganoderma lucidum.

Fungal Genet Biol 2019 Feb 14;123:70-77. Epub 2018 Dec 14.

Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China. Electronic address:

The fungal cell wall is very important for cell growth and survival during stress, and the target of rapamycin (TOR) pathway plays a major role in regulating cell growth in response to environmental cues. Ganoderma lucidum is an important edible and medicinal fungus, and the function of TOR in this organism remains unclear. As shown in the present study, the TOR pathway regulates cell wall integrity (CWI) in G. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.12.005DOI Listing
February 2019
2 Reads

Diverse members of the Xylariales lack canonical mating-type regions.

Fungal Genet Biol 2019 Jan 14;122:47-52. Epub 2018 Dec 14.

Department of Biology, University of New Mexico, Albuquerque, NM 87131 USA. Electronic address:

A survey of genomes reported here for 10 isolates of Monosporascus species and an additional 25 genomes from other members of the Xylariales (representing 15 genera) available in public databases indicated that genes typically associated with MAT1-1 (mat A) or MAT1-2 (mat a) mating types are absent or have diverged greatly relative to counterparts in other Pezizomycotina. This was particularly surprising for isolates known to be homothallic, given that homothallic members of the Pezizomycotina typically possess a MAT1-1-1 (mat A-1) gene and one or both of two other closely-linked mating-type genes, MAT1-1-2 (mat A-2) and MAT1-1-3 (mat A-3), in addition to MAT1-2-1 (mat a-1). We failed to detect candidate genes for either MAT1-1-1 or MAT1-1-2 in any member of the Xylariales. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.12.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321786PMC
January 2019

COPI localizes to the early Golgi in Aspergillus nidulans.

Fungal Genet Biol 2019 Feb 11;123:78-86. Epub 2018 Dec 11.

Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas CSIC, Ramiro de Maeztu 9, Madrid 28040, Spain. Electronic address:

Coatomer-I (COPI) is a heteromeric protein coat that facilitates the budding of membranous carriers mediating Golgi-to-ER and intra-Golgi transport. While the structural features of COPI have been thoroughly investigated, its physiological role is insufficiently understood. Here we exploit the amenability of A. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.12.003DOI Listing
February 2019
6 Reads

Reference genes for accurate normalization of gene expression in wood-decomposing fungi.

Fungal Genet Biol 2019 Feb 5;123:33-40. Epub 2018 Dec 5.

Department of Plant and Microbial Biology, University of Minnesota, Saint Paul, MN, United States. Electronic address:

Wood-decomposing fungi efficiently decompose plant lignocellulose, and there is increasing interest in characterizing and perhaps harnessing the fungal gene regulation strategies that enable wood decomposition. Proper interpretation of these fungal mechanisms relies on accurate quantification of gene expression, demanding reliable internal control genes (ICGs) as references. Commonly used ICGs such as actin, however, fluctuate among wood-decomposing fungi under defined conditions. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.11.005DOI Listing
February 2019
7 Reads

The mating system of the Eucalyptus canker pathogen Chrysoporthe austroafricana and closely related species.

Fungal Genet Biol 2019 Feb 4;123:41-52. Epub 2018 Dec 4.

Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria, South Africa.

Fungi in the genus Chrysoporthe are economically important canker pathogens of commercially grown Eucalyptus species and native Myrtales. Before the current study, homothallism was widely accepted as the mating system of these species, but this hypothesis could not be fully tested. Using whole genome sequences, we characterized the MAT locus of two C. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.12.001DOI Listing
February 2019

Automated, continuous video microscopy tracking of hyphal growth.

Fungal Genet Biol 2019 Feb 30;123:25-32. Epub 2018 Nov 30.

Division of Advanced Materials, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José 2055, 78216 San Luis Potosí, Mexico; Division of Molecular Biology, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José 2055, 78216 San Luis Potosí, Mexico. Electronic address:

The growth of filamentous fungi is a complex process that involves hyphal elongation and branching. Microscopic observations provide a wealth of information on fungal growth, although often requiring laborious manual intervention to record and analyze images. Here, we introduce a novel tool for automated tracking of growth in fungal hyphae that affords quantitative analysis of growth rate and morphology. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.11.006DOI Listing
February 2019
8 Reads

Neurospora crassa family GH72 glucanosyltransferases function to crosslink cell wall glycoprotein N-linked galactomannan to cell wall lichenin.

Fungal Genet Biol 2019 Feb 29;123:60-69. Epub 2018 Nov 29.

Department of Biological Sciences, SUNY University at Buffalo, Buffalo, NY 14260, USA. Electronic address:

The formation of a glucan/chitin/glycoprotein cell wall matrix is vital for fungal survival, growth, and morphogenesis. The cell wall proteins are important cell wall components and function in adhesion, signal transduction, and as cell wall structural elements. In this report we demonstrate that Neurospora crassa GH72 glucan transferases function to crosslink cell wall glycoproteins into the cell wall. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.11.007DOI Listing
February 2019
1 Read

l-Arabinose induces d-galactose catabolism via the Leloir pathway in Aspergillus nidulans.

Fungal Genet Biol 2019 Feb 26;123:53-59. Epub 2018 Nov 26.

Department of Biochemical Engineering, Faculty of Science and Technology, University of Debrecen, Egyetem tér 1, H-4032 Debrecen, Hungary. Electronic address:

l-Arabinose and d-galactose are the principal constituents of l-arabinogalactan, and also co-occur in other hemicelluloses and pectins. In this work we hypothesized that similar to the induction of relevant glycoside hydrolases by monomers liberated from these plant heteropolymers, their respective catabolisms in saprophytic and phytopathogenic fungi may respond to the presence of the other sugar to promote synergistic use of the complex growth substrate. We showed that these two sugars are indeed consumed simultaneously by Aspergillus nidulans, while l-arabinose is utilised faster in the presence than in the absence of d-galactose. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.11.004DOI Listing
February 2019

Fusarium graminearum KP4-like proteins possess root growth-inhibiting activity against wheat and potentially contribute to fungal virulence in seedling rot.

Fungal Genet Biol 2019 Feb 19;123:1-13. Epub 2018 Nov 19.

US Department of Agriculture, Agricultural Research Service, Cereal Crops Research Unit, Fargo, ND 58102-2765, USA.

The virally encoded KP4 killer toxin protein was first identified from Ustilago maydis (Um), and its homologues are present in diverse fungi and in one species of moss. No KP4-like (KP4L) proteins have been functionally characterized. Here, we report the identification and functional analysis of four KP4L proteins from Fusarium graminearum (Fg), the primary causal pathogen of Fusarium head blight (FHB), which is also known to associate with seedling rot of wheat. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.11.002DOI Listing
February 2019
5 Reads
2.587 Impact Factor

The putative H3K36 demethylase BcKDM1 affects virulence, stress responses and photomorphogenesis in Botrytis cinerea.

Fungal Genet Biol 2019 Feb 13;123:14-24. Epub 2018 Nov 13.

Institute of Plant Biology and Biotechnology, University of Münster, Schlossplatz 8, 48143 Münster, Germany.

The Leotiomycete Botrytis cinerea is a high-impact plant pathogen causing gray mold disease in a wide range of dicotyledonous species. Besides its efficient strategies to cause disease - either by being highly aggressive leading to rapid destruction of plant tissues or by keeping hidden for certain periods before damaging the host - the fungus is well-adapted to the changing environmental conditions due to different modes of reproduction for dispersal (macroconidia), survival (sclerotia) or adaptation (ascospores formed in the apothecia). The screening of a collection of B. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.11.003DOI Listing
February 2019
3 Reads

Role of Trichoderma arundinaceum tri10 in regulation of terpene biosynthetic genes and in control of metabolic flux.

Fungal Genet Biol 2019 Jan 12;122:31-46. Epub 2018 Nov 12.

Area of Microbiology, University of León, Campus de Ponferrada, Ponferrada, Spain. Electronic address:

Production of trichothecene toxins occurs in phylogenetically diverse fungi with different lifestyles. In these fungi, most homologs of the trichothecene biosynthetic gene cluster include the transcription factor genes tri6 and tri10. Analyses of phytopathogenic species of Fusarium indicate that the TRI6 and TRI10 proteins positively regulate genes required for synthesis of trichothecenes as well as farnesyl diphosphate (FPP), a precursor of the trichothecene and other terpenoids (e. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10871845183019
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http://dx.doi.org/10.1016/j.fgb.2018.11.001DOI Listing
January 2019
10 Reads

Overexpression of Schizosaccharomyces pombe tRNA 3'-end processing enzyme Trz2 leads to an increased cellular iron level and apoptotic cell death.

Fungal Genet Biol 2019 Jan 27;122:11-20. Epub 2018 Oct 27.

Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, 1 Wen Yuan Rd, Nanjing 210023, China. Electronic address:

The fission yeast Schizosaccharomyces pombe has two tRNase Z genes (trz1 and trz2) involved in nuclear and mitochondrial tRNA 3'-end processing, respectively. Overexpression of trz2 but not trz1 is toxic to cells. In the present work, we showed that trz2 overexpression led to apoptotic cell death, as revealed by DAPI and Annexin V-FITC staining. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.10.003DOI Listing
January 2019
7 Reads
2.590 Impact Factor

Aspergillus nidulans protein kinase C forms a complex with the formin SepA that is involved in apical growth and septation.

Fungal Genet Biol 2019 Jan 2;122:21-30. Epub 2018 Nov 2.

Biochemistry and Molecular Biology Program, Rhodes College, 2000 N. Parkway, Memphis, TN 38112, USA; Department of Biology, Rhodes College, 2000 N. Parkway, Memphis, TN 38112, USA.

The Aspergillus nidulans orthologue of Protein kinase C (PkcA) and the A. nidulans formin SepA participate in polarized growth. PkcA localizes to growing hyphal apices and septation sites, and amino acid sequences within PkcA that are required for PkcA to localize to these sites of cell wall synthesis have been identified. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.10.002DOI Listing
January 2019

The acetate uptake transporter family motif "NPAPLGL(M/S)" is essential for substrate uptake.

Fungal Genet Biol 2019 Jan 16;122:1-10. Epub 2018 Oct 16.

Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, Braga 4710-057, Portugal. Electronic address:

Organic acids are recognized as one of the most prevalent compounds in ecosystems, thus the transport and assimilation of these molecules represent an adaptive advantage for organisms. The AceTr family members are associated with the active transport of organic acids, namely acetate and succinate. The phylogenetic analysis shows this family is dispersed in the tree of life. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10871845183012
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http://dx.doi.org/10.1016/j.fgb.2018.10.001DOI Listing
January 2019
12 Reads

A glucuronoxylomannan-like glycan produced by Trichosporon mucoides.

Fungal Genet Biol 2018 Dec 27;121:46-55. Epub 2018 Sep 27.

Instituto de Microbiologia Paulo de Góes (IMPG), Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; Instituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil. Electronic address:

Trichosporon asahii shares with Cryptococcus species the ability to produce glucuronoxylomannan (GXM), an immunomodulatory fungal polysaccharide. The ability of other opportunistic species of Trichosporon to produce GXM-like polysaccharides is unknown. In this study, we observed that T. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10871845183021
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http://dx.doi.org/10.1016/j.fgb.2018.09.009DOI Listing
December 2018
13 Reads

The contribution of the White Collar complex to Cryptococcus neoformans virulence is independent of its light-sensing capabilities.

Fungal Genet Biol 2018 Dec 25;121:56-64. Epub 2018 Sep 25.

School of Biological Sciences, University of Missouri-Kansas City, MO 64110, USA; School of BioSciences, The University of Melbourne, VIC 3010, Australia. Electronic address:

The White Collar complex is responsible for sensing light and transmitting that signal in many fungal species. In Cryptococcus neoformans and C. deneoformans the complex is involved in protection against damage from ultraviolet (UV) light, repression of mating in response to light, and is also required for virulence. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.09.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252129PMC
December 2018
2 Reads

The power of discussion: Support for women at the fungal Gordon Research Conference.

Fungal Genet Biol 2018 Dec 24;121:65-67. Epub 2018 Sep 24.

Department of Plant Biology, Michigan State University, East Lansing, MI 48824-1312, USA.

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https://linkinghub.elsevier.com/retrieve/pii/S10871845183020
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http://dx.doi.org/10.1016/j.fgb.2018.09.007DOI Listing
December 2018
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Mgm1 is required for maintenance of mitochondrial function and virulence in Candida albicans.

Fungal Genet Biol 2018 Nov 18;120:42-52. Epub 2018 Sep 18.

Ministry of Education Key Laboratory of Molecular Microbiology and Technology, Department of Microbiology, College of Life Science, Nankai University, Tianjin 300071, China. Electronic address:

Mitochondria are dynamic organelles, and their shapes and sizes are regulated by mitochondrial fusion and fission. The proteins essential for mitochondrial fusion in Candida albicans have not been clearly characterized. In this study, Mgm1 was explored for its roles in mitochondrial function, cell cycle, hyphal growth and virulence in this pathogen. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.09.006DOI Listing
November 2018

Functional analysis of MoSnf7 in Magnaporthe oryzae.

Fungal Genet Biol 2018 Dec 18;121:29-45. Epub 2018 Sep 18.

Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, and Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture and Rural Affairs, Nanjing 210095, China. Electronic address:

Snf7 is the core subunit protein of the yeast endosomal sorting complex required for transport (ESCRT) complex, which plays important roles in endocytosis and autophagy. In this study, we characterized MoSnf7 in Magnaporthe oryzae, a homolog of yeast Snf7, the core protein of ESCRT-III subcomplex. Like Snf7, MoSnf7 also localizes next to the vacuoles. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.09.005DOI Listing
December 2018

Catabolic repression in early-diverging anaerobic fungi is partially mediated by natural antisense transcripts.

Fungal Genet Biol 2018 Dec 15;121:1-9. Epub 2018 Sep 15.

Department of Chemical Engineering, University of California, Santa Barbara, Santa Barbara, CA 93106, USA. Electronic address:

Early-diverging anaerobic fungi (order: Neocallimastigomycota), lignocelluolytic chytrid-like fungi central to fiber degradation in the digestive tracts of large herbivores, are attractive sources of cellulases and hemicellulases for biotechnology. Enzyme expression is tightly regulated and coordinated through mechanisms that remain unelucidated to optimize hydrolytic efficiency. Our analysis of anaerobic fungal transcriptomes reveals hundreds of cis-natural antisense transcripts (cis-NATs), which we hypothesize play an integral role in this regulation. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10871845183011
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http://dx.doi.org/10.1016/j.fgb.2018.09.004DOI Listing
December 2018
13 Reads

UmTea1, a Kelch and BAR domain-containing protein, acts at the cell cortex to regulate cell morphogenesis in the dimorphic fungus Ustilago maydis.

Fungal Genet Biol 2018 Dec 8;121:10-28. Epub 2018 Sep 8.

Department of Biological Sciences, California State University, 1250 Bellflower Boulevard, Long Beach, CA 90840, United States. Electronic address:

The spatial organization of a cell is crucial for distribution of cell components and for cell morphogenesis in all organisms. Ustilago maydis, a basidiomycete fungus, has a yeast-like and a filamentous form. The former buds once per cell cycle at one of the cell poles, and can use the same site repeatedly or choose a new site at the same pole or opposite pole. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.09.002DOI Listing
December 2018
11 Reads

Role of the small GTPase Rho1 in cell wall integrity, stress response, and pathogenesis of Aspergillus fumigatus.

Fungal Genet Biol 2018 Nov 8;120:30-41. Epub 2018 Sep 8.

Institute for Disease Control and Prevention of PLA, Beijing, China. Electronic address:

Aspergillus fumigatus is a major pathogen of invasive pulmonary aspergillosis. The small GTPase, Rho1, of A. fumigatus is reported to comprise a potential regulatory subunit of β-1,3-glucan synthase and is indispensable for fungal viability; however, the role of AfRho1 on the growth, cell wall integrity, and pathogenesis of A. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.09.003DOI Listing
November 2018
4 Reads

CORT0C04210 is required for Candida orthopsilosis adhesion to human buccal cells.

Fungal Genet Biol 2018 Nov 8;120:19-29. Epub 2018 Sep 8.

Department of Biology, University of Pisa, Pisa, Italy. Electronic address:

Candida orthopsilosis is a human fungal pathogen belonging to the Candida parapsilosis sensu lato species complex. C. orthopsilosis annotated genome harbors 3 putative agglutinin-like sequence (ALS) genes named CORT0B00800, CORT0C04210 and CORT0C04220. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.09.001DOI Listing
November 2018
30 Reads

Why outcross? The abandon-ship hypothesis in a facultative outcrossing/selfing fungal species.

Fungal Genet Biol 2018 Nov 1;120:1-8. Epub 2018 Sep 1.

Institut de Systématique, Evolution, Biodiversité (ISYEB), Muséum national d'Histoire naturelle, CNRS, Sorbonne Université, EPHE. CP 75005, 57 rue Cuvier, 75005 Paris, France. Electronic address:

Most species able to reproduce both sexually and asexually (facultative sexual species) invest more in sexual reproduction in stressful environment conditions. According to the abandon-ship hypothesis, plasticity for investment in sexual reproduction may have been selected in these species, allowing unfit genotypes to generate progeny carrying new advantageous allelic combinations. We tested this hypothesis in Aspergillus nidulans, a fungus able to reproduce asexually, or sexually, by outcrossing and/or haploid selfing (i. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.08.005DOI Listing
November 2018

The epigenetic reader SntB regulates secondary metabolism, development and global histone modifications in Aspergillus flavus.

Fungal Genet Biol 2018 Nov 18;120:9-18. Epub 2018 Aug 18.

Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI, USA; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA. Electronic address:

Due to the role, both beneficial and harmful, that fungal secondary metabolites play in society, the study of their regulation is of great importance. Genes for any one secondary metabolite are contiguously arranged in a biosynthetic gene cluster (BGC) and subject to regulation through the remodeling of chromatin. Histone modifying enzymes can place or remove post translational modifications (PTM) on histone tails which influences how tight or relaxed the chromatin is, impacting transcription of BGCs. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.08.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6215504PMC
November 2018

Secreted peroxidases VmPODs play critical roles in the conidiation, HO sensitivity and pathogenicity of Valsa mali.

Fungal Genet Biol 2018 10 18;119:20-28. Epub 2018 Aug 18.

College of Plant Protection and State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address:

Apple Valsa canker, caused by the necrotrophic pathogen Valsa mali, is a devastating disease of apples and causes great financial loss in East Asia. Improving the understanding of apple - V. mali interactions will contribute to disease management. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10871845183016
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http://dx.doi.org/10.1016/j.fgb.2018.08.003DOI Listing
October 2018
3 Reads

Effect of deletion of a trichothecene toxin regulatory gene on the secondary metabolism transcriptome of the saprotrophic fungus Trichoderma arundinaceum.

Fungal Genet Biol 2018 10 17;119:29-46. Epub 2018 Aug 17.

Area of Microbiology, University of León, Campus de Ponferrada, Ponferrada, Spain. Electronic address:

Trichothecenes are terpenoid toxins produced by multiple fungal species with diverse lifestyles. In these fungi, the trichothecene biosynthetic gene (tri) cluster includes a gene encoding a CysHis Zn-finger protein (TRI6). Analyses of plant pathogenic Fusarium species indicate that tri6 regulates tri gene expression. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.08.002DOI Listing
October 2018
10 Reads