2,250 results match your criteria Fungal Genetics and Biology [Journal]


CRISPR-Cas9-mediated disruption of the HMG-CoA reductase genes of Mucor circinelloides and subcellular localization of the encoded enzymes.

Fungal Genet Biol 2019 Apr 13. Epub 2019 Apr 13.

MTA-SZTE "Lendület" Fungal Pathogenicity Mechanisms Research Group, Közép fasor 52., 6726 Szeged, Hungary; Interdisciplinary Excellence Centre, Department of Microbiology, University of Szeged, Közép fasor 52., 6726 Szeged, Hungary; Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52., 6726 Szeged, Hungary. Electronic address:

Terpenoid compounds, such as sterols, carotenoids or the prenyl groups of various proteins are synthesized via the mevalonate pathway. A rate-limiting step of this pathway is the conversion of 3-methylglutaryl-CoA (HMG-CoA) to mevalonic acid catalyzed by the HMG-CoA reductase. Activity of this enzyme may affect several biological processes, from the synthesis of terpenoid metabolites to the adaptation to various environmental conditions. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.04.008DOI Listing
April 2019
4 Reads

Chromatin modification factors in plant pathogenic fungi: insights from Ustilago maydis.

Fungal Genet Biol 2019 Apr 10. Epub 2019 Apr 10.

Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide, de Sevilla-Consejo Superior de Investigaciones Científicas-Junta de Andalucía, Sevilla, Spain.

Adaptation to the environment is a requirement for the survival of every organism. For pathogenic fungi this also implies coping with the different conditions that occur during the infection cycle. After detecting changes to external media, organisms must modify their gene expression patterns in order to accommodate the new circumstances. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.04.006DOI Listing

Mutations in the Phosphatase Domain of the 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase Result in the Transcriptional Activation of the Alternative Oxidase and Gluconeogenic Pathways in Podospora anserina.

Fungal Genet Biol 2019 Apr 10. Epub 2019 Apr 10.

I2BC, UMR 9198, Université Paris-Saclay, Gif-sur-Yvette, France.

By screening suppressors of a respiratory mutant lacking a functional cytochrome pathway in the filamentous fungus Podospora anserina, we isolated a mutation located in the phosphatase domain of the bi-functional enzyme 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase (PFK-2/FBPase-2). We show that the inactivation of the phosphatase but not of the kinase domain is responsible for the suppressor effect that results from the activation of the RSEs transcription factors that control expression of AOX, an alternative oxidase able to bypass the mitochondria cytochrome pathway of respiration. Remarkably, activation of the RSEs also stimulates the expression of the gluconeogenic enzymes, fructose-1,6 bi-phosphatase (FBPase-1) and phosphoenolpyruvate carboxykinase (PCK-1). Read More

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http://dx.doi.org/10.1016/j.fgb.2019.04.005DOI Listing

Identification of the gene cluster for bistropolone-humulene meroterpenoid biosynthesis in Phoma sp.

Fungal Genet Biol 2019 Apr 10;129:7-15. Epub 2019 Apr 10.

State Key Laboratory of Toxicology & Medical Countermeasures, Beijing Institute of Pharmacology & Toxicology, Beijing 100850, China; Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China. Electronic address:

Eupenifeldin, a bistropolone meroterpenoid, was first discovered as an antitumor agent from the fungus Eupenicillium brefeldianum. We also isolated this compound and a new congener from a strain of Phoma sp. (CGMCC 10481), and evaluated their antitumor effects. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.04.004DOI Listing

The role of fungi in C. difficile infection: An underappreciated transkingdom interaction.

Fungal Genet Biol 2019 Apr 10;129:1-6. Epub 2019 Apr 10.

Department of Molecular Biology and Microbiology, Tufts University, Boston, MA 02111, USA. Electronic address:

Novel culture independent technologies have further elucidated the composition of the human mycobiome, though the role of fungi in human health and disease remains largely unknown. Recent studies have suggested conflicting roles for fungi in the gastrointestinal tract, underscoring the complexity of the interactions between the mycobiome, its bacterial counterpart, and the host. One key example is the observation that fungal taxa are overrepresented in patients with Clostridioides difficile infection (CDI), suggesting a role for fungi in this disease. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10871845183033
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http://dx.doi.org/10.1016/j.fgb.2019.04.007DOI Listing
April 2019
1 Read

The role of GYP-3 in cellular morphogenesis of Neurospora crassa: Analyzing its relationship with the polarisome.

Fungal Genet Biol 2019 Apr 5;128:49-59. Epub 2019 Apr 5.

Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada, Ensenada, BC 22860, Mexico. Electronic address:

In fungal hyphae multiple protein complexes assemble at sites of apical growth to maintain cell polarity. The polarisome, which in Saccharomyces cerevisiae consists of Spa2, Pea2, Bud6 and Bni1 is described as a small network of functionally related proteins that regulate polarized growth. In yeast Msb3 and Msb4 are considered polarisome components since both proteins interact directly with Spa2 and are involved in Bni1-nucleated actin assembly in vivo. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.04.003DOI Listing
April 2019
2 Reads

Genome-wide analysis of Fusarium verticillioides reveals inter-kingdom contribution of horizontal gene transfer to the expansion of metabolism.

Fungal Genet Biol 2019 Apr 3;128:60-73. Epub 2019 Apr 3.

USDA, ARS, US National Poultry Research Center, Toxicology & Mycotoxin Research Unit, Athens, GA, USA. Electronic address:

Horizontal gene transfer (HGT) is believed to shape genomes by facilitating the rapid acquisition of adaptive traits. We hypothesized that the economically important fungus Fusarium verticillioides is an excellent candidate for investigating the potential impact of HGT on the expansion of metabolic activities given its soilborne nature and versatile lifestyle as both a symptomless endophyte as well as a maize pathogen. To test this hypothesis, we used a phylogenomic pipeline followed by manual curation to perform a genome-wide identification of inter-kingdom derived HGT events. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.04.002DOI Listing
April 2019
1 Read

Shedding light on the mechanisms underlying the environmental regulation of secondary metabolite ganoderic acid in Ganoderma lucidum using physiological and genetic methods.

Fungal Genet Biol 2019 Apr 2;128:43-48. Epub 2019 Apr 2.

Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing 210095, Jiangsu, PR China. Electronic address:

The secondary metabolites of fungi are often produced at very low concentrations, and until recently the regulatory mechanisms of secondary metabolite biosynthesis have been unclear. Ganoderma lucidum is a macrofungus that is widely used as a traditional Chinese medicine or medicinal mushroom: ganoderic acid (GA) is one of the main active ingredients. Here, we review research from the last decade on which and how environmental factors regulate GA biosynthesis. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.03.009DOI Listing

Rapid discovery and functional characterization of diterpene synthases from basidiomycete fungi by genome mining.

Fungal Genet Biol 2019 Mar 21;128:36-42. Epub 2019 Mar 21.

School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, People's Republic of China; School of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, People's Republic of China. Electronic address:

Basidiomycete fungi are a rich source of bioactive diterpenoid secondary metabolites. However, compared with the large number of diterpene synthases (di-TPSs) identified in plants and ascomycete fungi, only three di-TPSs have been described from basidiomycete fungi. Large scale genome sequencing projects combined with the development of synthetic biology techniques now has enabled the rapidly discovery and characterization of di-TPSs from basidiomycete fungi. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10871845183029
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http://dx.doi.org/10.1016/j.fgb.2019.03.007DOI Listing
March 2019
11 Reads
2.587 Impact Factor

Breastmilk and NICU surfaces are potential sources of fungi for infant mycobiomes.

Fungal Genet Biol 2019 Mar 21;128:29-35. Epub 2019 Mar 21.

University of Minnesota, Department of Pediatrics, MMC319, 420 Delaware Street S.E., Minneapolis, MN 55455, USA. Electronic address:

Surfaces within the neonatal intensive care unit (NICU), especially those handled frequently by hospital staff, provide sources of gut-colonizing bacteria for hospitalized infants, in addition to those acquired perinatally from maternal sources such as breastmilk. In comparison to bacteria, very little is known about potential sources of colonizing fungi in the NICU setting. Thus, the objective of this study was to characterize fungal communities (mycobiomes) of potential colonization sources for neonates hospitalized in a large university NICU. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10871845183027
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http://dx.doi.org/10.1016/j.fgb.2019.03.008DOI Listing
March 2019
5 Reads

Aspergillus fumigatus Mnn9 is responsible for mannan synthesis and required for covalent linkage of mannoprotein to the cell wall.

Fungal Genet Biol 2019 Mar 20;128:20-28. Epub 2019 Mar 20.

State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Guangxi Academy of Sciences, Nanning 530007, Guangxi, China. Electronic address:

Owing to the essential role in protection of the Aspergillus fumigatus cell against human defense reactions, its cell wall has long been taken as a promising antifungal target. The cell wall of A. fumigatus composed of chitin, glucan and galactomannan and mannoproteins. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.03.006DOI Listing

The mitogen-activated protein kinase gene CcPmk1 is required for fungal growth, cell wall integrity and pathogenicity in Cytospora chrysosperma.

Fungal Genet Biol 2019 Mar 13;128:1-13. Epub 2019 Mar 13.

College of Forestry, Beijing Forestry University, Beijing, China. Electronic address:

Cytospora chrysosperma, the causal agent of canker disease in a wide range of woody plants, results in significant annual economic and ecological losses. Mitogen-activated protein kinase (MAPK) cascades are highly conserved signal transduction pathways that play a crucial role in mediating cellular responses to environmental and host signals in plant pathogenic fungi. In this study, we identified an ortholog of the Fus3/Kss1-related MAPK gene, CcPmk1, and characterized its functions in C. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.03.005DOI Listing
March 2019
1 Read

Rapid loss of virulence during submergence of Z. tritici asexual spores.

Fungal Genet Biol 2019 Mar 12;128:14-19. Epub 2019 Mar 12.

Geoffrey Pope Building, University of Exeter, Stocker Road, Exeter EX4 4QD, UK. Electronic address:

Zymoseptoria tritici, the causal agent of Septoria tritici blotch, is a notable pathogen of temperate-grown wheat. To better understand the mechanisms underpinning pathogenicity, leaf infection assays are commonly used to compare either the virulence of Z. tritici wildtype or mutant strains, or the susceptibility of wheat cultivars. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.03.004DOI Listing

Colonization of legumes by an endophytic Fusarium solani strain FsK reveals common features to symbionts or pathogens.

Fungal Genet Biol 2019 Mar 11;127:60-74. Epub 2019 Mar 11.

Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, Larissa 41500, Greece. Electronic address:

Plant cellular responses to endophytic filamentous fungi are scarcely reported, with the majority of described colonization processes in plant-fungal interactions referring to either pathogens or true symbionts. Fusarium solani strain K (FsK) is a root endophyte of Solanum lycopersicum, which protects against root and foliar pathogens. Here, we investigate the association of FsK with two legumes (Lotus japonicus and Medicago truncatula) and report on colonization patterns and plant responses during the establishment of the interaction. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10871845183025
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http://dx.doi.org/10.1016/j.fgb.2019.03.003DOI Listing
March 2019
4 Reads

SUMOlock reveals a more complete Aspergillus nidulans SUMOylome.

Fungal Genet Biol 2019 Mar 5;127:50-59. Epub 2019 Mar 5.

Department of Molecular Biosciences, The University of Kansas, 1200 Sunnyside Ave., Lawrence, KS 66045, USA.

SUMOylation, covalent attachment of the small ubiquitin-like modifier protein SUMO to proteins, regulates protein interactions and activity and plays a crucial role in the regulation of many key cellular processes. Understanding the roles of SUMO in these processes ultimately requires identification of the proteins that are SUMOylated in the organism under study. The filamentous fungus Aspergillus nidulans serves as an excellent model for many aspects of fungal biology, and it would be of great value to determine the proteins that are SUMOylated in this organism (i. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10871845193004
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http://dx.doi.org/10.1016/j.fgb.2019.03.002DOI Listing
March 2019
2 Reads

Profound mycobiome differences between segregated mouse colonies do not influence Th17 responses to a newly introduced gut fungal commensal.

Fungal Genet Biol 2019 Mar 5;127:45-49. Epub 2019 Mar 5.

Gastroenterology and Hepatology Division, Joan and Sanford I. Weill Department of Medicine, Weill Cornell Medicine, Cornell University, New York, NY 10021, USA; The Jill Roberts Institute for Research in Inflammatory Bowel Disease, Weill Cornell Medicine, Cornell University, New York, NY 10021, USA; Department of Microbiology and Immunology, Weill Cornell Medicine, Cornell University, New York, NY 10065, USA; Immunology and Microbial Pathogenesis Program, Weill Cornell Graduate School of Medical Sciences, Weill Cornell Medicine, Cornell University, New York, NY 10065, USA. Electronic address:

Gut mycobiota dysbiosis can negatively impact the outcome of several diseases of inflammatory origin, suggesting a role of the mycobiota in influencing the host immunity. However, it is unknown whether the gut mycobiota composition can create an immune environment that would influence the immune response to a newly introduced intestinal fungus. Using ITS1 deep sequencing, we evaluated the mycobiome structure of C57BL/6J mice acquired from Jackson (JAX) or bred in a controlled environment at a dedicated room in our own mouse facility (WCM-CE) for several generations. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.03.001DOI Listing

Disruption of putative short-chain acyl-CoA dehydrogenases compromised free radical scavenging, conidiogenesis, and pathogenesis of Magnaporthe oryzae.

Fungal Genet Biol 2019 Feb 27;127:23-34. Epub 2019 Feb 27.

State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian University Key Laboratory for Plant-Microbe Interaction, The School of Life Sciences, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address:

Short-chain acyl-CoA dehydrogenase (Scad) mediated β-oxidation serves as the fastest route for generating essential energies required to support the survival of organisms under stress or starvation. In this study, we identified three putative SCAD genes in the genome of the globally destructive rice blast pathogen Magnaporthe oryzae, named as MoSCAD1, MoSCAD2, and MoSCAD3. To elucidate their function, we deployed targeted gene deletion strategy to investigate individual and the combined influence of MoSCAD genes on growth, stress tolerance, conidiation and pathogenicity of the rice blast fungus. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.02.010DOI Listing
February 2019

The velvet protein VeA functions in asexual cycle, stress tolerance and transcriptional regulation of Beauveria bassiana.

Fungal Genet Biol 2019 Feb 23;127:1-11. Epub 2019 Feb 23.

MOE Laboratory of Biosystems Homeostasis & Protection, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China. Electronic address:

VeA is a key velvet protein that regulates sexual/asexual development and secondary metabolism in filamentous fungi, particularly Aspergilli, but has not been explored yet in asexual insect mycopathogens, such as Beauveria bassiana. Here, we report a localization of B. bassiana VeA in the cytoplasm of hyphal cells exposed to either light or dark cue and its migration to the nucleus only in darkness. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.02.009DOI Listing
February 2019

Heterologous expression of an alternative oxidase from Moniliophthora perniciosa in Saccharomyces cerevisiae: Antioxidant function and in vivo platform for the study of new drugs against witches' broom disease.

Fungal Genet Biol 2019 May 19;126:50-55. Epub 2019 Feb 19.

Laboratório de Biologia Molecular e Biotecnologia Industrial de Microrganismos, Departamento de Tecnologia Bioquímico-Farmacêutica, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, SP 05508-000, Brazil. Electronic address:

The fungus Moniliophthora perniciosa is the causal agent of witches' broom disease (WBD), one of the most devastating diseases of cacao, the chocolate tree. Many strategies to control WBD have been tested so far, including the use of agrochemicals such as the strobilurins. Strobilurins are fungicides of the QoI family, and they are used in the control of a wide array of fungal diseases in many different crops, including cereals, field crops, fruits, tree nuts, and vegetables. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.02.006DOI Listing
May 2019
1 Read

Genotypic and phenotypic characterization of Candida albicans Lebanese hospital isolates resistant and sensitive to caspofungin.

Fungal Genet Biol 2019 Feb 20;127:12-22. Epub 2019 Feb 20.

Department of Natural Sciences, Lebanese American University, PO Box 36, Byblos, Lebanon. Electronic address:

The fungus Candida albicans is both a commensal and an opportunistic human pathogen, present as part of the normal human microflora causing serious mucosal, and systemic life threatening infections. The antifungal drug caspofungin of the echinocandin family is the latest generation of antifungal drugs to be developed. It functions by inhibiting glucan synthase thus weakening the fungal cell wall leading to death. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.02.008DOI Listing
February 2019

The smut fungus Ustilago esculenta has a bipolar mating system with three idiomorphs larger than 500 kb.

Fungal Genet Biol 2019 May 19;126:61-74. Epub 2019 Feb 19.

Department of Plant Pathology and Microbiology, National Taiwan University, Taipei 10617, Taiwan, ROC. Electronic address:

Zizania latifolia Turcz., which is mainly distributed in Asia, has had a long cultivation history as a cereal and vegetable crop. On infection with the smut fungus Ustilago esculenta, Z. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.02.007DOI Listing
May 2019
1 Read

A strikingly-angled spindle mediates nuclear migration during colonization of rice cells infected by Magnaporthe oryzae.

Fungal Genet Biol 2019 May 19;126:56-60. Epub 2019 Feb 19.

Department of Plant Biology, University of Georgia, Athens, GA 30602, USA. Electronic address:

To cause rice blast disease, Magnaporthe oryzae must properly organize microtubules and position nuclei during colonization of host cells. Live cell confocal imaging of fluorescently-tagged microtubules and nuclei of M. oryzae invasive hyphae reveals that microtubules form a cage-like arrangement around nuclei during interphase and that the mitotic spindle forms and mediates nuclear migration while integrity of the nuclear envelope is lost. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.02.005DOI Listing
May 2019
1 Read

A unique Zn(II)-Cys-type protein, KpeA, is involved in secondary metabolism and conidiation in Aspergillus oryzae.

Fungal Genet Biol 2019 Feb 18;127:35-44. Epub 2019 Feb 18.

Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan. Electronic address:

Aspergillus oryzae is an important microorganism in the bio- and food industries; therefore, understanding the mechanism underlying its secondary metabolism regulation is important for ensuring its safe use. Here, we screened a novel Zn(II)-Cys-type protein-encoding gene, AO090003001186, designated as kpeA (kojic acid production enhancement A), from an A. oryzae disruption mutant library of transcriptional regulators. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.02.004DOI Listing
February 2019
1 Read

Chlorophyll fluorescence imaging for monitoring effects of Heterobasidion parviporum small secreted protein induced cell death and in planta defense gene expression.

Fungal Genet Biol 2019 May 11;126:37-49. Epub 2019 Feb 11.

Faculty of Agriculture and Forestry, P. O. Box 27, Latokartanonkaari 7, 00014, University of Helsinki, Helsinki, Finland; Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland. Electronic address:

Heterobasidion parviporum Niemelä & Korhonen is a necrotrophic fungal pathogen of Norway spruce (Picea abies). The H. parviporum genome encodes numerous necrotrophic small secreted proteins (SSP) which might be important for promoting and sustaining the disease development. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.02.003DOI Listing
May 2019
2.587 Impact Factor

CRISPR/Cas9-mediated endogenous gene tagging in Fusarium oxysporum.

Fungal Genet Biol 2019 May 6;126:17-24. Epub 2019 Feb 6.

Department of Entomology and Plant Pathology, Auburn University, Auburn Alabama 36849, United States. Electronic address:

Fusarium oxysporum is an economically important pathogen that widely exists in the environment and is capable of causing serious problems in crop production and animal/human health. One important step for characterization of a fungal protein with an unknown function is to determine its subcellular localization within the cell. To facilitate the study of important functional regulators or key virulence factors, we developed a CRISPR/Cas9-mediated endogenous gene tagging (EGT) system based on two different strategies, homology-independent targeted integration (HITI) and homology-dependent recombination integration (HDRI). Read More

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http://dx.doi.org/10.1016/j.fgb.2019.02.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456373PMC
May 2019
5 Reads

Core components of endosomal mRNA transport are evolutionarily conserved in fungi.

Fungal Genet Biol 2019 May 6;126:12-16. Epub 2019 Feb 6.

Heinrich Heine University Düsseldorf, Institute for Microbiology, Cluster of Excellence on Plant Sciences, 40204 Düsseldorf, Germany. Electronic address:

Active movement of mRNAs by sophisticated transport machineries determines precise spatiotemporal expression of encoded proteins. A prominent example discovered in fungi is microtubule-dependent transport via endosomes. This mode of transport was thought to be only operational in the basidiomycete Ustilago maydis. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.013DOI Listing

Transcriptional profiling and localization of GUL-1, a COT-1 pathway component, in Neurospora crassa.

Fungal Genet Biol 2019 May 4;126:1-11. Epub 2019 Feb 4.

Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 761001, Israel.

Impairment of theNeurospora crassaCOT-1 kinase results in defects in hyphal polarity. Some of these effects are partially suppressed by inactivation of gul-1 (encoding an mRNA-binding protein involved in translational regulation). Here, we report on the transcriptional profiling of cot-1 inactivation and demonstrate that gul-1 affects transcript abundance of multiple genes in the COT-1 pathway, including processes such as cell wall remodeling, nitrogen and amino acid metabolism. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.010DOI Listing

Complex epigenetic regulation of alkaloid biosynthesis and host interaction by heterochromatin protein I in a fungal endophyte-plant symbiosis.

Fungal Genet Biol 2019 Apr 4;125:71-83. Epub 2019 Feb 4.

School of Fundamental Sciences and the BioProtection Research Centre, Massey University, Private Bag 11 222, Palmerston North 4442, New Zealand. Electronic address:

Epichloë festucae forms mutualistic symbiotic interactions with grasses of the Lolium and Festuca genera. Protection from insect and mammalian herbivory are the best-documented host benefits of these associations. The two main classes of anti-mammalian alkaloids synthesized by E. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.02.001DOI Listing
April 2019
1 Read

Genomic comparisons of the laurel wilt pathogen, Raffaelea lauricola, and related tree pathogens highlight an arsenal of pathogenicity related genes.

Fungal Genet Biol 2019 Apr 1;125:84-92. Epub 2019 Feb 1.

Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO 80523, USA. Electronic address:

Raffaelea lauricola is an invasive fungal pathogen and symbiont of the redbay ambrosia beetle (Xyleborus glabratus) that has caused widespread mortality to redbay (Persea borbonia) and other Lauraceae species in the southeastern USA. We compare two genomes of R. lauricola (C2646 and RL570) to seven other related Ophiostomatales species including R. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.012DOI Listing

Unique gene Pmhyp controlling melanization of pycnidia in Phoma medicaginis.

Fungal Genet Biol 2019 Apr 30;125:53-59. Epub 2019 Jan 30.

Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, USA.

Phoma medicaginis (syn. Ascochyta medicaginicola Qchen & L. Cai) causes spring black stem and leaf spot of alfalfa and the model legume Medicago truncatula. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.007DOI Listing

Comparative transcriptome analysis reveals regulatory networks and key genes of microsclerotia formation in the cotton vascular wilt pathogen.

Fungal Genet Biol 2019 May 30;126:25-36. Epub 2019 Jan 30.

Chongqing Engineering Research Center of Specialty Crop Resources and The College of Life Science, Chongqing Normal University, Chongqing 401331, China. Electronic address:

Verticillium dahliae is a soil-borne, hemibiotrophic phytopathogenic fungus that causes Verticillium wilt in a broad range of economic crops. The microsclerotia (MS), which act as the main host inoculum, can survive long-term in soil resulting in uncontrollable disease. In order to clarify the mechanism of MS formation, we sequenced the whole genome-wide expression profile of V. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.009DOI Listing

The interplay between transport and metabolism in fungal itaconic acid production.

Fungal Genet Biol 2019 Apr 28;125:45-52. Epub 2019 Jan 28.

iAMB - Institute of Applied Microbiology, ABBt - Aachen Biology and Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany; Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich, Wilhelm-Johnen-Strasse, 52425 Jülich, Germany. Electronic address:

Besides enzymatic conversions, many eukaryotic metabolic pathways also involve transport proteins that shuttle molecules between subcellular compartments, or into the extracellular space. Fungal itaconate production involves two such transport steps, involving an itaconate transport protein (Itp), and a mitochondrial tricarboxylate transporter (Mtt). The filamentous ascomycete Aspergillus terreus and the unicellular basidiomycete Ustilago maydis both produce itaconate, but do so via very different molecular pathways, and under very different cultivation conditions. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10871845183022
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http://dx.doi.org/10.1016/j.fgb.2019.01.011DOI Listing
April 2019
5 Reads

Mating-type loci of Ustilago esculenta are essential for mating and development.

Fungal Genet Biol 2019 Apr 24;125:60-70. Epub 2019 Jan 24.

Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, China. Electronic address:

Ustilago esculenta is closely related to the smut fungus Ustilago maydis and, in an endophytic-like life in the plant Zizania latifolia, only infects host stems and causes swollen stems to form edible galls called Jiaobai in China. In order to study its different modes of invasion and sites of symptom development from other smut fungi at the molecular level, we first characterized the a and b mating-type loci of U. esculenta. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.008DOI Listing
April 2019
1 Read

Expression of the Fusarium graminearum terpenome and involvement of the endoplasmic reticulum-derived toxisome.

Fungal Genet Biol 2019 Mar 18;124:78-87. Epub 2019 Jan 18.

USDA ARS Cereal Disease Laboratory, Saint Paul, MN, USA. Electronic address:

The sesquiterpenoid deoxynivalenol (DON) is an important trichothecene mycotoxin produced by the cereal pathogen Fusarium graminearum. DON is synthesized in specialized subcellular structures called toxisomes. The first step in DON synthesis is catalyzed by the sesquiterpene synthase (STS), Tri5 (trichodiene synthase), resulting in the cyclization of farnesyl diphosphate (FPP) to produce the sesquiterpene trichodiene. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.006DOI Listing
March 2019
3 Reads

Influence of farming practices on the population genetics of the maize pathogen Cercospora zeina in South Africa.

Fungal Genet Biol 2019 Apr 16;125:36-44. Epub 2019 Jan 16.

Department of Plant and Soil Sciences, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, South Africa. Electronic address:

Gray leaf spot (GLS) is an important foliar disease of maize. This disease, caused by Cercospora zeina, is prevalent in both smallholder and commercial maize farms in South Africa. Notably, smallholder practices are geared towards conservation agriculture, planting diverse maize genotypes within a field and avoiding chemical control. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.005DOI Listing
April 2019
1 Read

Universal plasmids to facilitate gene deletion and gene tagging in filamentous fungi.

Fungal Genet Biol 2019 Apr 11;125:28-35. Epub 2019 Jan 11.

Faculty of Health Sciences, University of Macau, Avenida da Universidade, Taipa, Macau; Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Avenida da Universidade, Taipa, Macau. Electronic address:

Gene manipulation is an important routine technique and its efficiency is often a rate-limiting step in research. To facilitate gene manipulation in filamentous fungi, we adapted the S. cerevisiae Gene Deletion and Gene Tagging plasmid collections to include additional selectable markers that make the useful resources applicable to other fungi. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.004DOI Listing
April 2019
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Phosphoproteomic and transcriptomic analyses reveal multiple functions for Aspergillus nidulans MpkA independent of cell wall stress.

Fungal Genet Biol 2019 Apr 9;125:1-12. Epub 2019 Jan 9.

Department of Chemical, Biochemical, and Environmental Engineering, University of Maryland Baltimore County, Baltimore, MD 21250, United States. Electronic address:

The protein kinase MpkA plays a prominent role in the cell wall integrity signaling (CWIS) pathway, acting as the terminal MAPK activating expression of genes which encode cell wall biosynthetic enzymes and other repair functions. Numerous studies focus on MpkA function during cell wall perturbation. Here, we focus on the role MpkA plays outside of cell wall stress, during steady state growth. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.003DOI Listing
April 2019
2 Reads

The CysHis zinc finger protein Zfp1 regulates sexual reproduction and virulence in Cryptococcus neoformans.

Fungal Genet Biol 2019 Mar 7;124:59-72. Epub 2019 Jan 7.

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory of Microsporidia Infection and Prevention, Southwest University, Chongqing 400715, China. Electronic address:

Cryptococcus neoformans is a ubiquitous yeast pathogen that often infects the human central nervous system (CNS) to cause meningitis in immunocompromised individuals. Although numerous signaling pathways and factors important for fungal sexual reproduction and virulence have been investigated, their precise mechanism of action remains to be further elucidated. In this study, we identified and characterized a novel zinc finger protein Zfp1 that regulates fungal sexual reproduction and virulence in C. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.002DOI Listing
March 2019
2 Reads

The actin motor MYO-5 effect in the intracellular organization of Neurospora crassa.

Fungal Genet Biol 2019 Apr 4;125:13-27. Epub 2019 Jan 4.

Departamento de Microbiología, Centro de Investigación Científica y de Educación Superior de Ensenada, Ensenada, Baja California, Mexico. Electronic address:

In filamentous fungi, polarized growth is the result of vesicle secretion at the hyphal apex. Motor proteins mediate vesicle transport to target destinations on the plasma membrane via actin and microtubule cytoskeletons. Myosins are motor proteins associated with actin filaments. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10871845183019
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http://dx.doi.org/10.1016/j.fgb.2018.11.008DOI Listing
April 2019
12 Reads

Phosphatidate phosphatase Pah1 has a role in the hyphal growth and virulence of Candida albicans.

Fungal Genet Biol 2019 Mar 4;124:47-58. Epub 2019 Jan 4.

Key Laboratory of Cell Proliferation and Regulation Biology, College of Life Sciences, Beijing Normal University, Beijing, China. Electronic address:

Phosphatidate phosphatases play essential roles in lipid metabolism by converting phosphatidic acid to diacylglycerol. Here, we have investigated the roles of a phosphatidate phosphatase, Pah1, in the fungal pathogen Candida albicans. Deleting PAH1 causes multiple phenotypes, especially severe hyphal defects, increased sensitivity to cell wall stress, and reduced virulence in mice. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10871845183017
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http://dx.doi.org/10.1016/j.fgb.2018.12.010DOI Listing
March 2019
10 Reads

Proteomic characterization of Aspergillus fumigatus isolated from air and surfaces of the International Space Station.

Fungal Genet Biol 2019 Mar 3;124:39-46. Epub 2019 Jan 3.

Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA, USA. Electronic address:

The on-going Microbial Observatory Experiments on the International Space Station (ISS) revealed the presence of various microorganisms that may be affected by the distinct environment of the ISS. The low-nutrient environment combined with enhanced irradiation and microgravity may trigger changes in the molecular suite of microorganisms leading to increased virulence and resistance of microbes. Proteomic characterization of two Aspergillus fumigatus strains, ISSFT-021 and IF1SW-F4, isolated from HEPA filter debris and cupola surface of the ISS, respectively, is presented, along with a comparison to well-studied clinical isolates Af293 and CEA10. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10871845193000
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http://dx.doi.org/10.1016/j.fgb.2019.01.001DOI Listing
March 2019
11 Reads

Mating type (MAT) locus and possible sexuality of the opportunistic pathogen Exophiala dermatitidis.

Fungal Genet Biol 2019 Mar 3;124:29-38. Epub 2019 Jan 3.

Division of Mycology, Department of Microbiology, Faculty of Medicine, Çukurova University, Adana, Turkey. Electronic address:

Sexual reproduction among the black yeasts is generally limited to environmental saprobic species and is rarely observed among opportunists in humans. To date, a complete sexual cycle has not been observed in Exophiala dermatitidis. In this study, we aimed to gain insight into the reproductive mode of E. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.12.011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417501PMC
March 2019
15 Reads

Nanoscale enrichment of the cytosolic enzyme trichodiene synthase near reorganized endoplasmic reticulum in Fusarium graminearum.

Fungal Genet Biol 2019 Mar 21;124:73-77. Epub 2018 Dec 21.

USDA ARS Cereal Disease Laboratory, 1551 Lindig Street, St. Paul, MN 55108, USA; Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN 55108, USA. Electronic address:

Trichothecene mycotoxin synthesis in the phytopathogen Fusarium graminearum involves primarily endoplasmic reticulum (ER)-localized enzymes of the mevalonate- and trichothecene biosynthetic pathways. Two exceptions are 3-hydroxy-3-methylglutaryl CoA synthase (Hms1) and trichodiene synthase (Tri5), which are known cytosolic enzymes. Using 3D structured illumination microscopy (3D SIM), GFP-tagged Tri5 and Hms1 were tested for preferential localization in the cytosol proximal to the ER. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.12.008DOI Listing
March 2019
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Differential roles, crosstalk and response to the Antifungal Protein AfpB in the three Mitogen-Activated Protein Kinases (MAPK) pathways of the citrus postharvest pathogen Penicillium digitatum.

Fungal Genet Biol 2019 Mar 21;124:17-28. Epub 2018 Dec 21.

Department of Food Biotechnology, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Avda Agustín Escardino 7, 46980 Paterna, Valencia, Spain. Electronic address:

Fungi have three mitogen-activated protein kinases (MAPKs): Kss1/Fus3 involved in the invasive growth and virulence of pathogens, Hog1 in response to osmotic stress, and Slt2/Mpk1 in response to cell wall (CW) stress. We conducted comparative analyses of these MAPKs in the phytopathogen Penicillium digitatum and studied their role in the mode of action of the novel self-antifungal protein AfpB. The sensitivity to different stresses of Δhog1 and the reduced growth of Δkss1 coincided with previous reports. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.12.006DOI Listing
March 2019
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Nutrient sensing-the key to fungal p53-like transcription factors?

Authors:
Margaret E Katz

Fungal Genet Biol 2019 Mar 21;124:8-16. Epub 2018 Dec 21.

Molecular and Cellular Biology, University of New England, Armidale, NSW 2351, Australia. Electronic address:

The mammalian tumour suppressor protein, p53, plays an important role in cell cycle control, DNA repair and apoptotic cell death. Transcription factors belonging to the "p53-like" superfamily are found exclusively in the Amorphea branch of eukaryotes, which includes animals, fungi and slime molds. Many members of the p53-like superfamily (proteins containing p53, Rel/Dorsal, T-box, STAT, Runt, Ndt80, and the CSL DNA-binding domains) are involved in development. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.12.007DOI Listing
March 2019
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CRISPR-Cas9 induces point mutation in the mucormycosis fungus Rhizopus delemar.

Fungal Genet Biol 2019 Mar 15;124:1-7. Epub 2018 Dec 15.

Department of Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center, New Orleans, LA, USA; Department of Pediatrics, Louisiana State University Health Sciences Center, New Orleans, LA, USA. Electronic address:

Rhizopus delemar causes devastating mucormycosis in immunodeficient individuals. Despite its medical importance, R. delemar remains understudied largely due to the lack of available genetic markers, the presence of multiple gene copies due to genome duplication, and mitotically unstable transformants resulting from conventional and limited genetic approaches. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.12.002DOI Listing
March 2019
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The Slt2-MAPK pathway is involved in the mechanism by which target of rapamycin regulates cell wall components in Ganoderma lucidum.

Fungal Genet Biol 2019 Feb 14;123:70-77. Epub 2018 Dec 14.

Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China. Electronic address:

The fungal cell wall is very important for cell growth and survival during stress, and the target of rapamycin (TOR) pathway plays a major role in regulating cell growth in response to environmental cues. Ganoderma lucidum is an important edible and medicinal fungus, and the function of TOR in this organism remains unclear. As shown in the present study, the TOR pathway regulates cell wall integrity (CWI) in G. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.12.005DOI Listing
February 2019
3 Reads

Diverse members of the Xylariales lack canonical mating-type regions.

Fungal Genet Biol 2019 01 14;122:47-52. Epub 2018 Dec 14.

Department of Biology, University of New Mexico, Albuquerque, NM 87131 USA. Electronic address:

A survey of genomes reported here for 10 isolates of Monosporascus species and an additional 25 genomes from other members of the Xylariales (representing 15 genera) available in public databases indicated that genes typically associated with MAT1-1 (mat A) or MAT1-2 (mat a) mating types are absent or have diverged greatly relative to counterparts in other Pezizomycotina. This was particularly surprising for isolates known to be homothallic, given that homothallic members of the Pezizomycotina typically possess a MAT1-1-1 (mat A-1) gene and one or both of two other closely-linked mating-type genes, MAT1-1-2 (mat A-2) and MAT1-1-3 (mat A-3), in addition to MAT1-2-1 (mat a-1). We failed to detect candidate genes for either MAT1-1-1 or MAT1-1-2 in any member of the Xylariales. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.12.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321786PMC
January 2019
1 Read

COPI localizes to the early Golgi in Aspergillus nidulans.

Fungal Genet Biol 2019 Feb 11;123:78-86. Epub 2018 Dec 11.

Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas CSIC, Ramiro de Maeztu 9, Madrid 28040, Spain. Electronic address:

Coatomer-I (COPI) is a heteromeric protein coat that facilitates the budding of membranous carriers mediating Golgi-to-ER and intra-Golgi transport. While the structural features of COPI have been thoroughly investigated, its physiological role is insufficiently understood. Here we exploit the amenability of A. Read More

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http://dx.doi.org/10.1016/j.fgb.2018.12.003DOI Listing
February 2019
11 Reads