31,836 results match your criteria Food Poisoning

Rapid and Accurate Detection With CRISPR-Cas12b Based on Newly Identified -Specific and -Conserved Genomic Signatures.

Front Microbiol 2021 27;12:649010. Epub 2021 Apr 27.

Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China.

is among the most prevalent foodborne zoonotic pathogens leading to diarrheal diseases. In this study, we developed a CRISPR-Cas12b-based system to rapidly and accurately detect contamination. Identification of -specific and -conserved genomic signatures is a fundamental step in development of the detection system. Read More

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Why knowledge is the best way to reduce the risks associated with raw milk and raw milk products.

J Dairy Res 2021 May 14:1-6. Epub 2021 May 14.

Mestrado em Ciência e Tecnologia de Leite e Derivados, Universidade Pitágoras-Unopar, LondrinaPR, Brazil.

In an age of flexible conditions about mandatory milk pasteurisation, this opinion-based research reflection supports the view that the knowledge and the awareness of milk-borne infections are key requirements to decrease the risks associated with raw milk. Providing an analysis of the current potential risks related to consumption of raw milk and raw milk products, we discuss the main reasons to continue to be vigilant about milk-borne pathogens and the current scenario in relation to the formal and clandestine sale of raw milk. Finally, we select some highly effective strategies to reduce the risks associated with raw milk in food services. Read More

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MALDI-TOF Mass Spectrometry and 16S rRNA Gene Sequence Analysis for the Identification of Foodborne Clostridium Spp.

J AOAC Int 2021 May 12. Epub 2021 May 12.

U.S. Food and Drug Administration, Microbiological Sciences Branch, Southeast Food and Feed Laboratory, Office of Regulatory Science, Office of Regulatory Affairs, 60 8th Street NE, Atlanta, GA, 30309, USA.

Background: Clostridium is a genus of Gram-positive, spore-forming, anaerobic bacteria comprising approximately 100 species. Some Clostridium spp. (C. Read More

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Travel-associated human trichinellosis in Portugal.

IDCases 2021 17;24:e01124. Epub 2021 Apr 17.

Department of Infectious Diseases, Hospital de São Bernardo, Centro Hospitalar de Setúbal, R. Camilo Castelo Branco 175, 2910-548, Setúbal, Portugal.

Trichinellosis is an emerging or re-emerging foodborne parasitic zoonotic disease caused by nematodes of the genus . It represents a global food safety problem and a public health hazard. This case report aims to describe the first case of human trichinellosis in Portugal since the creation of the European Union surveillance system. Read More

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Evaluation of 24-h screen deployments as a standardized platform to monitor Gambierdiscus populations in the Florida Keys and U.S. Virgin Islands.

Harmful Algae 2021 Mar 13;103:101998. Epub 2021 Feb 13.

Biology Department, MS #32, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA.

Anchored mesh screens have been suggested as a standardized approach to monitor the cell abundances of epiphytic dinoflagellates in benthic habitats, including toxigenic members of the Gambierdiscus genus responsible for ciguatera poisoning (CP). Here we deployed screens for 24h at eight sites in the Florida Keys and St. Thomas (US Virgin Islands) to evaluate their performance relative to the traditional method of assessing Gambierdiscus abundance in which cell counts are normalized to wet weight of host algae. Read More

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Improving in vitro ciguatoxin and brevetoxin detection: selecting neuroblastoma (Neuro-2a) cells with lower sensitivity to ouabain and veratridine (OV-LS).

Harmful Algae 2021 Mar 2;103:101994. Epub 2021 Feb 2.

German Federal Institute for Risk Assessment, Department Safety in the Food Chain, National Reference Laboratory of Marine Biotoxins, Max-Dohrn-Str. 8-10, 10589 Berlin, Germany - www.bfr.bund.de.

Marine biotoxins accumulating in seafood products pose a risk to human health. These toxins are often potent in minute amounts and contained within complex matrices; requiring sensitive, reliable, and robust methods for their detection. The mouse neuroblastoma (Neuro-2a) cytotoxicity assay (N2a-assay) is a sensitive, high-throughput, in vitro method effective for detecting sodium channel-specific marine biotoxins. Read More

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Trends in recent waterborne and foodborne disease outbreaks in South Korea, 2015-2019.

Osong Public Health Res Perspect 2021 Apr 29;12(2):73-79. Epub 2021 Apr 29.

Infectious Disease Control, Center for Infectious Disease Control, Korea Disease Control and Prevention Agency, Cheongju, Korea.

Objective: This study analyzed trends in foodborne and waterborne diseases in South Korea between 2015 and 2019.

Methods: The data consisted of information on outbreaks of waterborne and foodborne infectious diseases reported through the Korea Centers for Disease Control and Prevention (KCDC) system. We analyzed the trends and epidemiological aspects of outbreaks by month, place of occurrence, and causative pathogens in this observational study. Read More

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Genomic Analysis of Antibiotic-Resistant and -Susceptible Isolated from Bovine Sources in Maputo, Mozambique.

Foodborne Pathog Dis 2021 May 11. Epub 2021 May 11.

Environmental Microbial and Food Safety Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland, USA.

This study reports a genomic analysis of isolates recovered from 25 bovine fecal composite samples collected from four different production units in Maputo city and around Maputo Province, Mozambique. The genomes were analyzed to determine the presence of antibiotic resistance genes (ARGs), genetic relatedness, and virulence factors known to cause diseases in humans. Whole-genome sequencing was conducted on 28 isolates using an Illumina NextSeq 500 sequencing platform. Read More

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Positive correlation between Candida auris skin-colonization burden and environmental contamination at a ventilator-capable skilled nursing facility in Chicago.

Clin Infect Dis 2021 May 12. Epub 2021 May 12.

Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.

Background: Candida auris is an emerging multidrug-resistant yeast that contaminates healthcare environments causing healthcare-associated outbreaks. The mechanisms facilitating contamination are not established.

Methods: C. Read More

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Knowledge, Attitude, Practice and Food Poisoning Associated Factors Among Parents in Bench-Sheko Zone, Southwest Ethiopia.

Int J Gen Med 2021 3;14:1673-1681. Epub 2021 May 3.

Department of Epidemiology and Biostatistics, Mizan-Tepi University, Mizan-Aman, Southern Nation Nationality and People Region, Ethiopia.

Background: Food poisoning is a food borne disease, mainly resulting from ingestion of food that contains a toxin, chemical or infectious microorganisms like bacteria, virus, parasite, or prion. On the other hand, avoiding food contamination during preparing and feeding is a key factor for reducing the prevalence of food poisoning. This study aimed to assess the knowledge, attitude, self-reported practice and food poisoning associated factors among parents in the selected health centers of Bench-Sheko Zone in Ethiopia. Read More

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Childhood lead exposure of Amerindian communities in French Guiana: an isotopic approach to tracing sources.

Environ Geochem Health 2021 May 11. Epub 2021 May 11.

Geosciences Environnement Toulouse (GET), Observatoire Midi-Pyrénées, Université de Toulouse, CNRS, 31400, Toulouse, IRD, France.

In French Guiana were detected high lead (Pb) levels in blood of Amerindian people. Lead exposure is a serious hazard that can affect the cognitive and behavior development. People can be exposed to Pb through occupational and environmental sources. Read More

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An immunoinformatics approach for the design of a multi-epitope vaccine targeting super antigen TSST-1 of Staphylococcus aureus.

J Genet Eng Biotechnol 2021 May 11;19(1):69. Epub 2021 May 11.

Department of Biotechnology, Vignan's Foundation for Science, Technology and Research (Deemed to be University), Vadlamudi, Guntur - District, Andhra Pradesh, 522 213, India.

Background: TSST-1 is a secretory and pyrogenic superantigen that is being responsible for staphylococcal mediated food poisoning and associated clinical manifestations. It is one of the main targets for the construction of vaccine candidates against Staphylococcus aureus. Most of the vaccines have met failure due to adverse reactions and toxicity reported during late clinical studies. Read More

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CRISPR/Cas12a-Based Versatile Method for Checking Quantitative Polymerase Chain Reaction Samples with Cycles of Threshold Values in the Gray Zone.

ACS Sens 2021 May 11. Epub 2021 May 11.

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.

Quantitative polymerase chain reaction (qPCR) is widely applied in foodborne pathogen detection and diagnosis. According to the cycles of threshold (Ct) values of qPCR testing, samples are judged as positive or negative. However, samples with Ct values in the gray zone are classified as "possibly positive" and required to be tested again. Read More

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The immune protection induced by a serine protease from the Trichinella spiralis adult against Trichinella spiralis infection in pigs.

PLoS Negl Trop Dis 2021 May 10;15(5):e0009408. Epub 2021 May 10.

Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, China.

Trichinellosis is a major foodborne parasitosis caused by Trichinella spiralis. In the present study, a serine protease gene from an adult T. spiralis (Ts-Adsp) cDNA library was cloned, expressed in Escherichia coli and purified by Ni-affinity chromatography. Read More

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Multiplex PCR method for simultaneous detection of five pathogenic bacteria closely related to foodborne diseases.

3 Biotech 2021 May 16;11(5):219. Epub 2021 Apr 16.

School of Medical Technology, Xuzhou Medical University, No. 209 Tongshan Road, Yunlong District, Xuzhou, 221004 China.

In this study, we describe a multiplex PCR method for the detection of five food-relevant virulence pathogenicity genes of intestinal pathogens. Five pairs of primers were designed based on gene for , gene of , gene of , gene of and gene of . Conditions were optimized to amplify fragments of those genes simultaneously in one PCR amplification. Read More

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Frequency of toxin genes and antibiotic resistance pattern of isolates in diarrheal samples among hospitalized patients in Hamadan, Iran.

Gastroenterol Hepatol Bed Bench 2021 ;14(2):165-173

Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.

Aim: This study was designed to investigate the prevalence of , its toxin-producing genes, and antibiotic resistance patterns in diarrheal samples from hospitalized patients in Hamadan, Iran.

Background: Today, concerns over infection (CDI) have significantly increased due to reduced susceptibility to antibiotics used for CDI treatment. Toxins produced by strains are associated with disease severity and outcome. Read More

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January 2021

Understanding the veterinary antibiotics supply chain to address antimicrobial resistance in Lao PDR: roles and interactions of involved stakeholders.

Acta Trop 2021 May 6:105943. Epub 2021 May 6.

UMR ASTRE, Université de Montpellier, CIRAD, INRAE, Montpellier, France; Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.

In response to the global call to mitigate risks associated with antimicrobial resistance (AMR), new regulations on the access and use of veterinary antibiotics are currently being developed by the Lao government. This study aims to explore how the implementation of these new regulations might effectively reduce and adapt the sale, distribution and use of veterinary antibiotics in Lao PDR. To this end, we used the theory of change, framing the AMR issue within the context of the stakeholder groups involved in the veterinary antibiotics supply chain. Read More

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Mucosal IFNγ production and potential role in protection in Escherichia coli O157:H7 vaccinated and challenged cattle.

Sci Rep 2021 May 7;11(1):9769. Epub 2021 May 7.

USDA-ARS, National Animal Disease Center, 1920 Dayton Avenue, P.O. Box 70, Ames, IA, 50010, USA.

Shiga-toxin producing Escherichia coli O157:H7 (O157)-based vaccines can provide a potential intervention strategy to limit foodborne zoonotic transmission of O157. While the peripheral antibody response to O157 vaccination has been characterized, O157-specific cellular immunity at the rectoanal junction (RAJ), a preferred site for O157 colonization, remains poorly described. Vaccine induced mucosal O157-specific antibodies likely provide some protection, cellular immune responses at the RAJ may also play a role in protection. Read More

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strains producing selected bacteriocins inhibit porcine enterotoxigenic (ETEC) both under and conditions.

Appl Environ Microbiol 2021 May 7. Epub 2021 May 7.

Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic

Enterotoxigenic (ETEC) and Shiga toxin-producing (STEC) strains are the causative agents of severe foodborne diseases in both humans and animals. In this study, porcine pathogenic strains (n = 277) as well as porcine commensal strains (n = 188) were tested for their susceptibility to 34 bacteriocin monoproducers to identify the most suitable bacteriocin types inhibiting porcine pathogens. Under condition, the set of pathogenic was found to be significantly more susceptible to a majority of tested bacteriocins when compared to commensal Based on production of bacteriocins with specific activity against pathogen, three potentially probiotic commensal strains of human origin were selected. Read More

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Assessing the risk of coal-burning arsenic-induced liver damage: a population-based study on hair arsenic and cumulative arsenic.

Environ Sci Pollut Res Int 2021 May 7. Epub 2021 May 7.

The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, School of Public Health, Guizhou Medical University, Guiyang, 550025, China.

Exposure to arsenic-contaminated air and food caused by the burning of coal in unventilated indoor stoves is a major environmental public health concern in Guizhou Province, China. The liver is one of the main target organs for coal-fired arsenic exposure; however, there is little information about the risk assessment between cumulative arsenic exposure and the prevalence of liver damage. This study first evaluated the chronic daily intake (CDI) for two exposure pathways (inhalation and ingestion) and five environmental media (i. Read More

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Comparative macrolide use in humans and animals: should macrolides be moved off the World Health Organisation's critically important antimicrobial list?

J Antimicrob Chemother 2021 May 6. Epub 2021 May 6.

Veterinary Medicine Research and Development, Zoetis, Inc, Kalamazoo, MI, USA.

Macrolide antibiotics are categorized by the WHO as Highest Priority, Critically Important Antimicrobials due to their recommendation as treatment for severe cases of campylobacteriosis in humans; a self-limiting, rarely life-threatening, zoonotic foodborne infection. Low rates of macrolide resistance in Campylobacter jejuni and the availability of alternative treatments have prompted some regulatory schemes to assign macrolides to a lower importance category. Apart from rare, specific infections, macrolides largely play a supportive role to other drug classes in human medicine. Read More

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Clinical Guidelines for Diagnosis and Treatment of Botulism, 2021.

MMWR Recomm Rep 2021 05 7;70(2):1-30. Epub 2021 May 7.

Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, CDC.

Botulism is a rare, neurotoxin-mediated, life-threatening disease characterized by flaccid descending paralysis that begins with cranial nerve palsies and might progress to extremity weakness and respiratory failure. Botulinum neurotoxin, which inhibits acetylcholine release at the neuromuscular junction, is produced by the anaerobic, gram-positive bacterium Clostridium botulinum and, rarely, by related species (C. baratii and C. Read More

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Sporulation is dispensable for the vegetable-associated life cycle of the human pathogen Bacillus cereus.

Microb Biotechnol 2021 May 6. Epub 2021 May 6.

Departamento de Microbiología, Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Universidad de Málaga, Bulevar Louis Pasteur 31 (Campus Universitario de teatinos), Málaga, 29071, Spain.

Bacillus cereus is a common food-borne pathogen that is responsible for important outbreaks of food poisoning in humans. Diseases caused by B. cereus usually exhibit two major symptoms, emetic or diarrheic, depending on the toxins produced. Read More

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Electrochemical Sensors to Detect Bacterial Foodborne Pathogens.

ACS Sens 2021 May 6. Epub 2021 May 6.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.

Bacterial foodborne pathogens cause millions of illnesses each year and disproportionately impact those in developing countries. To combat these diseases and their spread, effective monitoring of foodborne pathogens is needed. Technologies to detect these microbes must be deployable at the point-of-contamination, often in nonideal environments. Read More

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Integrating the Food and Drug Administration Office of the Coordinated Outbreak Response and Evaluation Network's foodborne illness outbreak surveillance and response activities with principles of the National Incident Management System.

J Emerg Manag 2021 Mar-Apr;19(2):131-141

Food and Drug Administration, Center for Food Safety and Applied Nutrition, Office of the Coordinated Outbreak Response and Evaluation Network, College Park, Maryland.

The Food Safety Modernization Act mandates building a national Integrated Food Safety System, which represents a seamless partnership among federal, state, local, territorial, and tribal agencies. During multistate foodborne illness outbreak investigations, local and state partners, the Centers for Disease Control and Prevention, the United States Food and Drug Administration (FDA), or the United States Department of Agriculture Food Safety Inspection Service, depending on the regulated food product, become engaged and assist in coordinating the efforts between partners involved and determine the allocation of resources. The FDA Center for Food Safety and Applied Nutrition (CFSAN) Office of the Coordinated Outbreak Response and Evaluation (CORE) Network coordinates foodborne illness outbreak surveillance, response, and post-response activities related to incidents involving multiple illnesses linked to FDA-regulated human food, dietary supplements, and cosmetic products. Read More

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Drastic hypothermia after intraperitoneal injection of okadaic acid, a diarrhetic shellfish poisoning toxin, in mice.

Hodaka Suzuki

Exp Anim 2021 Apr 29. Epub 2021 Apr 29.

Laboratory of Food Microbiology and Toxicology, College of Agriculture, Ibaraki University.

The mouse bioassay for diarrhetic shellfish poisoning (DSP) toxins had been used as the official method in Japan and also used in the world. In this study, hypothermia, one of the symptoms observed in mice after inoculation with DSP toxins, were characterized. Lethal and sublethal doses of okadaic acid (OA), a representative component of DSP toxins, were inoculated intraperitoneally into mice. Read More

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A systematic review and meta-analysis of Penner serotype prevalence of Campylobacter jejuni in low- and middle-income countries.

PLoS One 2021 5;16(5):e0251039. Epub 2021 May 5.

Enteric Diseases Department, Infectious Diseases Directorate, Naval Medical Research Center, Silver Spring, MD, United States of America.

Introduction: While Campylobacter jejuni is a leading foodborne bacterial pathogen worldwide, it poses a particular risk to susceptible populations in low- and middle-income countries (LMICs). A capsule-conjugate vaccine approach has been proposed as a potential solution, but little information exists on circulating C. jejuni capsule types in LMICs. Read More

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Trends in Agricultural Triazole Fungicide Use in the United States, 1992-2016 and Possible Implications for Antifungal-Resistant Fungi in Human Disease.

Environ Health Perspect 2021 May 5;129(5):55001. Epub 2021 May 5.

Mycotic Diseases Branch, Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.

Background: The fungus () is the leading cause of invasive mold infections, which cause severe disease and death in immunocompromised people. Use of triazole antifungal medications in recent decades has improved patient survival; however, triazole-resistant infections have become common in parts of Europe and are emerging in the United States. Triazoles are also a class of fungicides used in plant agriculture, and certain triazole-resistant strains found causing disease in humans have been linked to environmental fungicide use. Read More

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Classification of Cucumber Leaves Based on Nitrogen Content Using the Hyperspectral Imaging Technique and Majority Voting.

Plants (Basel) 2021 Apr 29;10(5). Epub 2021 Apr 29.

Faculty of Engineering, Autonomous University of Guerrero, Chilpancingo, Guerrero 39087, Mexico.

Improper usage of nitrogen in cucumber cultivation causes nitrate accumulation in the fruit and results in food poisoning in humans; therefore, mandatory evaluation of food products becomes inevitable. Hyperspectral imaging has a very good ability to evaluate the quality of fruits and vegetables in a non-destructive manner. The goal of the present paper was to identify excess nitrogen in cucumber plants. Read More

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Direct identification and molecular characterization of zoonotic hazards in raw milk by metagenomics using as a model pathogen.

Microb Genom 2021 May;7(5)

Department of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.

Metagenomics is a valuable diagnostic tool for enhancing microbial food safety because (i) it enables the untargeted detection of pathogens, (ii) it is fast since primary isolation of micro-organisms is not required, and (iii) it has high discriminatory power allowing for a detailed molecular characterization of pathogens. For shotgun metagenomics, total nucleic acids (NAs) are isolated from complex samples such as foodstuff. Along with microbial NAs, high amounts of matrix NAs are extracted that might outcompete microbial NAs during next-generation sequencing and compromise sensitivity for the detection of low abundance micro-organisms. Read More

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