112,168 results match your criteria Fluids and Barriers of the CNS [Journal]


Risk of Chlamydia abortus transmission via embryo transfer using in vitro produced early bovine embryos.

Theriogenology 2018 Dec 1;126:114-120. Epub 2018 Dec 1.

LUNAM University, Oniris, Nantes-Atlantic National College of Veterinary Medicine, Food Science and Engineering, Sanitary Security of Reproduction Biotechnology Unit, Nantes, France. Electronic address:

The objectives of this study were to determine (i) whether Chlamydia (C.) abortus would adhere to the intact zona pellucida (ZP-intact) of early in vitro produced bovine embryos; (ii) whether the bacteria would adhere to the embryos (ZP-free) after in vitro infection; and (iii) the efficacy of the International Embryo Transfer Society (IETS) washing protocol. The experimentation was made twice. Read More

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December 2018

A Special Risk Group for Hepatitis E Infection: The First Record of North Cyprus.

Pol J Microbiol 2018 ;67(4):525-528

Department of Biophysics, Faculty of Medicine, Near East University , Nicosia , Cyprus.

Hepatitis E virus (HEV) is transmitted by a fecal oral route from animals to humans following exposure to the body fluids of infected animals. We investigated the seroprevalence of anti-hepatitis E (anti-HEV) antibodies by monitoring IgG and IgM virus antibodies amongst employees in the animal industry in North Cyprus through a cross-sectional study. Samples were taken from individuals without occupational exposure to animals and from those who worked with animals (doing animal husbandry, veterinary work or butchery). Read More

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January 2018

Salivary creatinine and urea in patients with end-stage chronic kidney disease could not be used as diagnostic biomarkers for the effectiveness of dialysis treatment.

G Ital Nefrol 2018 Dec;35(6)

Department of Oral Surgery, Faculty of Dental medicine, Medical University, Plovdiv, Bulgaria.

Introduction: End-stage chronic kidney disease (CKD) is characterized by kidney failure with the organ's functions reduced or lost completely, where the kidneys are incapable of filtering excess fluids. Renal replacement therapy may be provided by peritoneal dialysis, hemodialysis or renal transplantation. Among the key indicators for tracking patients' current status are urea and creatinine levels. Read More

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December 2018

A Magnetic Gated Nanofluidic Based on the Integration of a Superhydrophilic Nanochannels and Reconfigurable Ferrofluid.

Adv Mater 2018 Dec 14:e1805953. Epub 2018 Dec 14.

University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.

The design of intelligent gating in nanoscale is the subject of intense research motivated by a broad potential impact on science and technology. However, the existing designs require complex modification and are unstable, which restrict their practical applications. Here, a magnetic gated nanofluidic is reported based on the integration of superhydrophilic membranes and reconfigurable ferrofluid, which realizes the gating of the nanochannel by adjusting the steric configuration of the ferrofluid. Read More

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December 2018

Endotracheal intubation results in acute tracheal damage induced by mtDNA/TLR9/NF-κB activity.

J Leukoc Biol 2018 Dec 13. Epub 2018 Dec 13.

Surgery, Washington University School of Medicine in Saint Louis, St. Louis, Missouri, USA.

Tracheitis secondary to placement of an endotracheal tube (ETT) is characterized by neutrophil accumulation in the tracheal lumen, which is generally associated with epithelial damage. Mitochondrial DNA (mtDNA), has been implicated in systemic inflammation and organ dysfunction following trauma; however, less is known about the effects of a foreign body on local trauma and tissue damage. We hypothesized that tracheal damage secondary to the ETT will result in local release of mtDNA at sufficient levels to induce TLR9 and NF-κB activation. Read More

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December 2018

Smart complex fluids based on two-antennary oligoglycines.

ChemSusChem 2018 Dec 12. Epub 2018 Dec 12.

BULGARIA.

Antennary oligoglycines are synthetic products, obtained by preliminary molecular design. Equal-length antennae are built of glycine residues jointed by C-end to oligoamine branching core with amine group at the N-terminus exposed outside. Results from a systematic research on properties of aqueous solutions containing two-antennary oligoglycine with four glycine portions are reported here. Read More

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December 2018

Empirical measurement of pressure in NPWT for infected wounds: how long can it really stay under pressure?

Wound Repair Regen 2018 Dec 12. Epub 2018 Dec 12.

Emergency Surgery Department, Università Cattolica del Sacro Cuore, Fondazione Policlinico A. Gemelli, Rome.

Surgical site infections (SSIs) represent one of the most common surgical complications. Negative pressure wound therapy is considered an effective wound management system, based on the principle that a negative pressure inside the wound can suction fluids and approximate wound edges. With the negative pressure wound therapy systems commercially available it is assumed that the pressure inside the wound is stable at the set values. Read More

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December 2018

3D Printed Microfluidic Mixers-A Comparative Study on Mixing Unit Performances.

Small 2018 Dec 10:e1804326. Epub 2018 Dec 10.

Institute of Technical Chemistry, Leibniz University Hannover, Callinstraße 5, 30167, Hannover, Germany.

One of the basic operations in microfluidic systems for biological and chemical applications is the rapid mixing of different fluids. However, flow profiles in microfluidic systems are laminar, which means molecular diffusion is the only mixing effect. Therefore, mixing structures are crucial to enable more efficient mixing in shorter times. Read More

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December 2018

On the sensitivity of alginate rheology to composition.

Soft Matter 2018 Dec 14. Epub 2018 Dec 14.

Institut für Organische Chemie, Universität Regensburg, Universitätsstr. 31, 93053 Regensburg, Germany and Instituto de Productos Naturales y Agrobiología del CSIC, Avda. Astrofísico Francisco Sánchez 3, 38206 La Laguna, Tenerife, Spain.

The linear response of alginate-phenyl boronic acid (Alg-PBA) esters shows a universal, composition-independent viscoelastic fluid-like behaviour. Reversible association of alginates governs their rheology at all compositions (viz. at all alginate concentrations and solution pH). Read More

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December 2018

Crossover between Athermal Jamming and the Thermal Glass Transition of Suspensions.

Phys Rev Lett 2018 Nov;121(22):228001

Institute of Physics, University of Amsterdam, Science Park 904, 1018 XH Amsterdam, Netherlands.

The non-Newtonian flow behavior of thermal and athermal disordered systems of dispersed uniform particles at high densities have strikingly similar features. By investigating the flow curves of yield-stress fluids and colloidal glasses having different volume fractions, particle sizes, and interactions, we show that both thermal and athermal systems exhibit power-law scaling with respect to the glass and jamming point, respectively, with the same exponents. All yield-stress flow curves can be scaled onto a single universal curve using the Laplace pressure as the stress scale for athermal systems and the osmotic pressure for the thermal systems. Read More

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November 2018

Case report and review of clenbuterol cardiac toxicity.

J Cardiol Cases 2013 Oct 31;8(4):131-133. Epub 2013 Aug 31.

Division of Cardiology, University of Alberta, Edmonton, Alberta, Canada.

Clenbuterol is an oral β2-agonist utilized as an illicit substance for performance-enhancement or weight loss. We report a case of a 23-year-old male who presented with anxiety and chest tightness after intentional ingestion of 5000 μg of clenbuterol (125 times the recommended adult dose) to lose weight. His electrocardiogram showed sinus tachycardia and diffuse nonspecific repolarization abnormalities with mild inferolateral ST-segment depression. Read More

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October 2013

Ventricular arrhythmias and acute left ventricular dysfunction as a primary and life-threatening manifestation of a mitochondrial crisis: A novel management strategy.

J Cardiol Cases 2012 Aug 30;6(2):e35-e38. Epub 2012 May 30.

Division of Cardiology, University of Texas Health Science Center, Houston, TX, USA.

Mitochondrial disorders are genetic diseases that result in a deficiency of energy metabolism (ATP production). A "mitochondrial crisis" can occur in the setting of infection, dehydration, or physiologic stress. The hallmark of a mitochondrial crisis is failure of multiple individual organ systems. Read More

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Imbalance of M1/M2 macrophages is linked to severity level of knee osteoarthritis.

Exp Ther Med 2018 Dec 11;16(6):5009-5014. Epub 2018 Oct 11.

Department of Emergency Surgery, The Affiliated Qingdao Hiser Hospital of Qingdao University (Qingdao Hospital of Traditional Chinese Medicine), Qingdao, Shandong 266001, P.R. China.

Macrophages, whether M1 or M2 subtype, have been found to be implicated in the pathogenesis of osteoarthritis (OA). However, no study regarding the status of M1 and M2 macrophages has been reported in knee OA. To investigate the status of M1 and M2 macrophages in knee OA, synovial fluid as well as peripheral blood were collected from 80 patients with knee OA and 80 healthy controls. Read More

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December 2018

Molecular evolution of juvenile hormone esterase-like proteins in a socially exchanged fluid.

Sci Rep 2018 Dec 13;8(1):17830. Epub 2018 Dec 13.

Center for Integrative Genomics, University of Lausanne, Lausanne, Switzerland.

Socially exchanged fluids are a direct means by which an organism can influence conspecifics. It was recently shown that when workers of the carpenter ant Camponotus floridanus feed larval offspring via trophallaxis, they transfer Juvenile Hormone III (JH), a key developmental regulator, as well as paralogs of JH esterase (JHE), an enzyme that catalyzes the hydrolysis of JH. Here we combine proteomic, phylogenetic and selection analyses to investigate the evolution of this esterase subfamily. Read More

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December 2018
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Development of hyponatremia in non-critical patients receiving total parenteral nutrition: A prospective, multicenter study.

Clin Nutr 2018 Nov 30. Epub 2018 Nov 30.

Endocrinology and Nutrition Department, Hospital Clínico Universitario de Valladolid-IEN, Universidad de Valladolid, Valladolid, Spain.

Background & Aims: Hyponatremia is frequent in hospitalized patients, especially in those receiving total parenteral nutrition (TPN). Furthermore, the presence of hyponatremia is associated with increased morbimortality in both groups. The goal of this study is to describe the prevalence of hyponatremia developing during TPN in non-critical patients, and identify risk factors for its appearance. Read More

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November 2018

Bioactive Glasses and Glass-Ceramics for Healthcare Applications in Bone Regeneration and Tissue Engineering.

Materials (Basel) 2018 Dec 12;11(12). Epub 2018 Dec 12.

Department of Materials and Ceramic Engineering, CICECO, University of Aveiro, 3810-193 Aveiro, Portugal.

The discovery of bioactive glasses (BGs) in the late 1960s by Larry Hench et al. was driven by the need for implant materials with an ability to bond to living tissues, which were intended to replace inert metal and plastic implants that were not well tolerated by the body. Among a number of tested compositions, the one that later became designated by the well-known trademark of 45S5 Bioglass excelled in its ability to bond to bone and soft tissues. Read More

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December 2018

Simultaneous Quantitation of Isoprenoid Pyrophosphates in Plasma and Cancer Cells Using LC-MS/MS.

Molecules 2018 Dec 11;23(12). Epub 2018 Dec 11.

Department of Pharmacy Practice, University of Nebraska Medical Center, Omaha, NE 68198, USA.

Isoprenoids (IsoP) are an important class of molecules involved in many different cellular processes including cholesterol synthesis. We have developed a sensitive and specific LC-MS/MS method for the quantitation of three key IsoPs in bio-matrices, geranyl pyrophosphate (GPP), farnesyl pyrophosphate (FPP), and geranylgeranyl pyrophosphate (GGPP). LC-MS/MS analysis was performed using a Nexera UPLC System connected to a LCMS-8060 (Shimadzu Scientific Instruments, Columbia, MD) with a dual ion source. Read More

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December 2018

A label-free aptamer-based nanogap capacitive biosensor with greatly diminished electrode polarization effects.

Phys Chem Chem Phys 2018 Dec 13. Epub 2018 Dec 13.

School of Mathematics and Physical Sciences, University of Hull, Hull, HU6 7RX, UK. and George W Gray Centre for Advanced Materials, University of Hull, Hull, HU6 7RX, UK.

A significant impediment to the use of impedance spectroscopy in bio-sensing is the electrode polarization effect that arises from the movement of free ions to the electrode-solution interface, forming an electrical double layer (EDL). The EDL screens the dielectric response of the bulk and its large capacitance dominates the signal response at low frequency, masking information particularly relevant for biological samples, such as molecular conformation changes and DNA hybridization. The fabrication of nanogap capacitors with electrode separation less than the EDL thickness can significantly reduce electrode polarization effects and provide enormous improvement in sensitivity due to better matching of the sensing volume with the size of the target entities. Read More

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December 2018

Structured illumination microscopy combined with machine learning for the high throughput analysis of virus structure.

Elife 2018 Dec 13;7. Epub 2018 Dec 13.

Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, United Kingdom.

Optical super-resolution microscopy techniques enable high molecular specificity with high spatial resolution and constitute a set of powerful tools in the investigation of the structure of supramolecular assemblies such as viruses. Here, we report on a new methodology which combines Structured Illumination Microscopy (SIM) with machine learning algorithms to image and classify the structure of large populations of biopharmaceutical viruses with high resolution. The method offers information on virus morphology that can ultimately be linked with functional performance. Read More

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December 2018

Choroid plexus genes for CSF production and brain homeostasis are altered in Alzheimer's disease.

Fluids Barriers CNS 2018 Dec 12;15(1):34. Epub 2018 Dec 12.

Department of Neurosurgery, Stanford University, 710 Frenchmans Rd, Stanford, CA, 94305, USA.

Background: The roles of the choroid plexus (CP) and cerebrospinal fluid (CSF) production have drawn increasing attention in Alzheimer's disease (AD) research. Specifically, studies document markedly decreased CSF production and turnover in moderate-to-severe AD. Moreover, reduced CP function and CSF turnover lead to impaired clearance of toxic metabolites, likely promote neuroinflammation, and may facilitate neuronal death during AD progression. Read More

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December 2018

Use of Salivary MicroRNAs for Diagnosis of Solid Cancers.

Klin Onkol 2018 ;31(4):249-259

Background: A modern approach to identify biomarkers of solid cancers in tissues and body fluids is based on microRNA (miRNA) expression profiling. miRNAs are a group of approximately 3.000 short noncoding RNAs containing 18-25 nucleotides that regulate gene expression at the post-transcriptional (mRNA) level. Read More

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January 2018

Effects of Chemical Speciation on the Bioaccessibility of Zinc in Spiked and Smelter-Affected Soils.

Environ Toxicol Chem 2018 Dec 7. Epub 2018 Dec 7.

Department of Soil Science, University of Saskatchewan, Saskatoon, SK, S7N 5A8, Canada.

Previous studies have suggested that understanding soil metal speciation, rather than relying solely on total metal content, can improve the accuracy and utility of contaminated site risk assessments. Since soil properties and reaction time can alter metal speciation, then speciation should influence metal bioaccessibility. For example, under gastrointestinal conditions, it is expected that metal species will differ in bioaccessibility depending upon their stability in acidic pH environments. Read More

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December 2018

Aberrant Metabolism in Hepatocellular Carcinoma Provides Diagnostic and Therapeutic Opportunities.

Oxid Med Cell Longev 2018 4;2018:7512159. Epub 2018 Nov 4.

Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy.

Hepatocellular carcinoma (HCC) accounts for over 80% of liver cancer cases and is highly malignant, recurrent, drug-resistant, and often diagnosed in the advanced stage. It is clear that early diagnosis and a better understanding of molecular mechanisms contributing to HCC progression is clinically urgent. Metabolic alterations clearly characterize HCC tumors. Read More

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November 2018

Influence of CO Degassing on the Microbial Community in a Dry Mofette Field in Hartoušov, Czech Republic (Western Eger Rift).

Front Microbiol 2018 21;9:2787. Epub 2018 Nov 21.

GFZ German Research Centre for Geosciences, Section Geomicrobiology, Potsdam, Germany.

The Cheb Basin (CZ) is a shallow Neogene intracontinental basin filled with fluvial and lacustrine sediments that is located in the western part of the Eger Rift. The basin is situated in a seismically active area and is characterized by diffuse degassing of mantle-derived CO in mofette fields. The Hartoušov mofette field shows a daily CO flux of 23-97 tons of CO released over an area of 0. Read More

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November 2018

Development and performance of a biomimetic artificial perilymph for in vitro testing of medical devices.

J Neural Eng 2018 Nov 28. Epub 2018 Nov 28.

Graduate School of Biomedical Engineering, University of New South Wales, Sydney, New South Wales, 2052, AUSTRALIA.

Objective: Cochlear implants interface with the fluid in the cochlea called perilymph. The volume of this fluid present in human and animal model cochlea is prohibitively low for isolation for in vitro studies. Thus, there is a need for an artificial perilymph that reflects the complexity of this fluid in terms of competitive protein adsorption. Read More

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November 2018

Shape deformation of a vesicle under an axisymmetric non-uniform alternating electric field.

J Phys Condens Matter 2019 Jan;31(3):035101

Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai, Maharashtra, India.

We suggest that non-uniform electric fields that are commonly used to study vesicle dielectrophoresis can be employed in hitherto relatively unexplored areas of vesicle deformation (for electromechanical characterization) and electroporation. Conventionally, the tension generated in vesicles is commonly modeled to be entropic or enthalpic in origin. A comparison of the configuration of a vesicle in the enthalpic and entropic regimes as well as the cross over between the two regimes during vesicle deformation has eluded understanding. Read More

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January 2019

Laser-activated microparticles for multimodal imaging: ultrasound and photoacoustics.

Phys Med Biol 2018 Nov 28. Epub 2018 Nov 28.

Physics of Fluids Group, Technical Medical (TechMed) Centre and MESA+ Institute for Nanotechnology, University of Twente, Enschede, NETHERLANDS.

The increasing personalization of medical treatment demands rened imaging and increased monitoring capabilities, as well as an improved efficacy through targeted drug delivery. Such a transition in health care can be facilitated by the use of multimodal contrast agents. In this paper, we present a novel type of multimodal contrast agents, that enhances contrast both in ultrasound and in photoacoustic imaging, while at the same time being capable of triggered drug delivery. Read More

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November 2018

Hyperammonaemia in classic organic acidaemias: a review of the literature and two case histories.

Orphanet J Rare Dis 2018 Dec 6;13(1):219. Epub 2018 Dec 6.

Department of Pediatrics, Division of Medical Genetics, University of Utah School of Medicine, 30 N 1900 E, Salt Lake City, UT, 84132, USA.

Background: The 'classic' organic acidaemias (OAs) (propionic, methylmalonic and isovaleric) typically present in neonates or infants as acute metabolic decompensation with encephalopathy. This is frequently accompanied by severe hyperammonaemia and constitutes a metabolic emergency, as increased ammonia levels and accumulating toxic metabolites are associated with life-threatening neurological complications. Repeated and frequent episodes of hyperammonaemia (alongside metabolic decompensations) can result in impaired growth and intellectual disability, the severity of which increase with longer duration of hyperammonaemia. Read More

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December 2018

Elevated Levels of Urinary Extracellular Vesicle Fibroblast-Specific Protein 1 in Patients with Active Crescentic Glomerulonephritis.

Nephron 2018 Dec 12:1-11. Epub 2018 Dec 12.

Department of Nephrology, Faculty of Medical Sciences, University of Fukui, Fukui, Japan.

Background/aims: Extracellular vesicles (EVs), including exosomes, are present in various bodily fluids, including urine. We and others previously reported that cells expressing fibroblast-specific protein 1 (FSP1) accumulate within damaged glomeruli, and that urinary FSP1, as well as urinary soluble CD163, could potentially serve as a biomarker of ongoing glomerular injury.

Methods: To test that idea, we collected urine samples from 37 patients with glomerular disease; purified the urinary EVs; characterized them using Nanosight, western blotting, and immunoelectron microscopy; and determined FSP1 and soluble CD163 levels using enzyme-linked immunosorbent assays. Read More

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December 2018

Antidiarrheal activity of α-terpineol in mice.

Biomed Pharmacother 2018 Dec 8;110:631-640. Epub 2018 Dec 8.

Medicinal Plants Research Center (NPPM), Federal University of Piauí, 64.049-550, Teresina, PI, Brazil. Electronic address:

Diarrhea is one of the leading causes of infant death in the world accounting for high child mortality rate. It is also present in different pathophysiologies related to several etiological agents. The aim of this study is to investigate the antidiarrheal effect of α -Terpineol (α-TPN) in different diarrhea models in rodents. Read More

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December 2018

Live nanoscopic to mesoscopic topography reconstruction with an optical microscope for chemical and biological samples.

PLoS One 2018 12;13(12):e0207881. Epub 2018 Dec 12.

LAI, Aix Marseille Univ, CNRS, INSERM, Marseille, France.

Macroscopic properties of physical and biological processes like friction, wetting, and adhesion or cell migration are controlled by interfacial properties at the nanoscopic scale. In an attempt to bridge simultaneously investigations at different scales, we demonstrate here how optical microscopy in Wet-Surface Ellipsometric Enhanced Contrast (Wet-SEEC) mode offers imaging and measurement of thin films at solid/liquid interfaces in the range 1-500 nm with lateral optical resolution. A live, label-free and noninvasive methodology integrated with microfluidic devices allowed here characterization of polymers and proteins patterns together with corresponding phenotypes of living cells. Read More

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December 2018

Antiemetic Drug Use in Children: What the Clinician Needs to Know.

J Pediatr Gastroenterol Nutr 2018 Dec 11. Epub 2018 Dec 11.

Clinical Pharmacology & Digestive Pathophysiology Unit, Department of Clinical & Experimental Medicine, University of Parma, Parma, Italy.

Vomiting is not only unpleasant for both children and families, but can lead to frequent hospital admission. The persistent vomiting hampers oral intake and increases the risk of dehydration so the proper use of antiemetic drugs can be useful. The pharmacological treatment of vomiting in children remains a challenge for the pediatrician because several antiemetics are prescribed as "off-label", outside their authorized drug label. Read More

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December 2018

Evaluation of levels of interleukin-6, interleukin-8 and some haematologic parameters of dogs with cutaneous wounds.

Cytokine 2019 Jan 28;113:128-138. Epub 2018 Jun 28.

Department of Veterinary Medicine, ABU, Zaria, Nigeria.

The aim of the study was to investigate the time-course of serum and wound fluids interleukin (IL)-6 and IL-8 levels in dogs with cutaneous wounds and their relationship with some haematologic parameters. The experimental group comprised of six adult dogs that underwent surgery with wounds (n = 6) on the mid lateral aspect of the right antebrachium; and control group of six, apparently, healthy intact (free from cutaneous wounds) adult dogs, comprising equal number of both sexes. Vital signs evaluated were within normal limits. Read More

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January 2019
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"I Don't Get No Respect" - The Role of Chloride in Acute Kidney Injury.

Am J Physiol Renal Physiol 2018 Dec 12. Epub 2018 Dec 12.

Department of Medicine, Icahn School of Medicine at Mount Sinai, United States.

Acute kidney injury (AKI) is a major public health problem that complicates 10-40% of hospital admissions. Importantly, AKI is independently associated with increased risk of progression to chronic kidney disease, end stage renal disease, cardiovascular events, and increased risk of in-hospital and long-term mortality. The chloride content of intravenous fluid has garnered much attention over the last decade, and its association with excess use and adverse outcomes including AKI. Read More

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December 2018

Miniaturized sample preparation methods for saliva analysis.

Bioanalysis 2019 Jan 12;11(2):119-148. Epub 2018 Dec 12.

Department of Chemistry, Faculty of Science, Qom University, Qom, Iran.

Saliva, as the first body fluid encountering with the exogenous materials, has good correlation with blood and plays an important role in bioanalysis. However, saliva has not been studied as much as the other biological fluids mainly due to restricted access to its large volumes. In recent years, there is a growing interest for saliva analysis owing to the emergence of miniaturized sample preparation methods. Read More

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January 2019

Fluid Preservation of Zoological Specimens.

Methods Mol Biol 2019 ;1897:135-148

School of Life Sciences, University of Nottingham, Nottingham, UK.

Preservation of organic materials in fluids for later dissection and education in biobanking stems from the need to prevent their degradation by the action of decomposers. Decomposers can be endogenous (e.g. Read More

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January 2019

Procurement and Storage of Pleural and Peritoneal Fluids for Biobanking.

Methods Mol Biol 2019 ;1897:125-133

Department of Pathology and Laboratory Medicine, Cedars Sinai Medical Center, Los Angeles, CA, USA.

There is limited information regarding the biobanking of pleural and peritoneal fluids that might supplement storage of pulmonary and thoracic tissue biospecimens. Such fluids are sometimes collected for clinical analyses and may have uses that obviate or supplement tissue samples. There has been a growing interest in using liquid biopsies as they are less invasive and may be amenable to analyses that guide targeted therapies. Read More

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January 2019

The Meningococcal Cysteine Transport System Plays a Crucial Role in Survival in Human Brain Microvascular Endothelial Cells.

MBio 2018 Dec 11;9(6). Epub 2018 Dec 11.

RIKEN Structural Biology Laboratory, Yokohama, Japan.

While typically exists in an asymptomatic nasopharyngeal carriage state, it may cause potentially lethal diseases in humans, such as septicemia or meningitis, by invading deeper sites in the body. Since the nutrient compositions of human cells are not always conducive to meningococci, needs to exploit nutrients from host environments. In the present study, the utilization of cysteine by the meningococcal cysteine transport system (CTS) was analyzed for the pathogenesis of meningococcal infections. Read More

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December 2018
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Biopolymers - Calcium phosphates composites with inclusions of magnetic nanoparticles for bone tissue engineering.

Int J Biol Macromol 2018 Dec 8. Epub 2018 Dec 8.

Grigore T. Popa University of Medicine and Pharmacy, Faculty of Medical Bioengineering, Department of Biomedical Sciences, Iasi, Romania. Electronic address:

Composites based on combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles have been prepared by a biomimetic co-precipitation method. The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids. Read More

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December 2018

Correspondence on "Preorganization and Cooperation for Highly Efficient and Reversible Capture of Low-Concentration CO by Ionic Liquids".

Angew Chem Int Ed Engl 2018 Dec 11. Epub 2018 Dec 11.

Institute of Chemistry-, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Porto Alegre, 91501-970, RS, Brazil.

The preorganization and cooperation mechanism of imide-based ionic liquids reported in a recent Communication was evocated to rationalize the extremely high gravimetric CO capture displayed by these fluids. An analysis of the reported spectroscopic evidences together with additional experiments led to the proposition of an alternative, simpler, and feasible mechanism involving the formation of bicarbonate. Read More

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December 2018

Highly sensitive spectrofluorimetric method for rapid determination of orciprenaline in biological fluids and pharmaceuticals.

Luminescence 2018 Dec 11. Epub 2018 Dec 11.

Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Mansoura University, Mansoura, Egypt.

Orciprenaline sulphate (ORP) is a direct-acting sympathomimetic with mainly beta-adrenoceptor stimulant activity. It is used as a bronchodilator in the management of reversible airway obstruction. For the first time, a rapid highly sensitive spectrofluorimetric method is described that is relied on measuring the fluorescence spectra of ORP at acidic pH and without addition of any chemical reagents. Read More

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December 2018

The temporomandibular joint: pneumatic temporal cells open into the articular and extradural spaces.

Folia Morphol (Warsz) 2018 Dec 11. Epub 2018 Dec 11.

The pneumatization of the articular tubercle (PAT) of the temporal squama is a rare condition that modifies the barrier between the temporomandibular joint (TMJ) space and the middle cranial fossa. During a routine examination of the cone beam computed tomography (CBCT) files of patients who were scanned for dental medical purposes, we identified a case with multiple rare anatomic variations. First, the petrous apex was bilaterally pneumatized. Read More

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December 2018

Hemodynamic effects of extended prone position sessions in ARDS.

Ann Intensive Care 2018 Dec 7;8(1):120. Epub 2018 Dec 7.

Service de Réanimation Médicale, Hôpital De La Croix Rousse, Hospices Civils de Lyon, 103 Grande Rue de la Croix Rousse, 69004, Lyon, France.

Background: Hemodynamic response to prone position (PP) has never been studied in a large series of patients with acute respiratory distress syndrome (ARDS). The primary aim of this study was to estimate the rate of PP sessions associated with cardiac index improvement. Secondary objective was to describe hemodynamic response to PP and during the shift from PP to supine position. Read More

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December 2018
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The GMO90+ project: absence of evidence for biologically meaningful effects of genetically modified maize based-diets on Wistar rats after 6-months feeding comparative trial.

Toxicol Sci 2018 Dec 10. Epub 2018 Dec 10.

Toxalim (Research Centre in Food Toxicology), Université de Toulouse, INRA, ENVT, INP Purpan, UPS, Toulouse, France.

The GMO90+ project was designed to identify biomarkers of exposure or health effects in Wistar Han RCC rats exposed in their diet to two genetically-modified plants (GMP) and assess additional information with the use of metabolomic and transcriptomic techniques. Rats were fed for six-months with 8 maize-based diets at 33% that comprised either MON810 (11% and 33%) or NK603 grains (11% and 33 % with or without glyphosate treatment) or their corresponding near-isogenic controls. Extensive chemical and targeted analyses undertaken to assess each diet demonstrated that they could be used for the feeding trial. Read More

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December 2018

IL-17 in Peritoneal Dialysis-Associated Inflammation and Angiogenesis: Conclusions and Perspectives.

Front Physiol 2018 26;9:1694. Epub 2018 Nov 26.

Department of Medicine I, Nephrology, Transplantation, Medical Intensive Care, University of Witten/Herdecke, Cologne-Merheim Medical Center, Cologne, Germany.

Long-term peritoneal dialysis (PD) is associated with peritoneal membrane remodeling. This includes changes in peritoneal vasculature, which may ultimately lead to inadequate solute and water removal and treatment failure. The potential cause of such alterations is chronic inflammation induced by repeated episodes of infectious peritonitis and/or exposure to bioincompatible PD fluids. Read More

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November 2018

An Integrated Cytoskeletal Model of Neurite Outgrowth.

Front Cell Neurosci 2018 26;12:447. Epub 2018 Nov 26.

Department of Biological Sciences, Purdue University, West Lafayette, IN, United States.

Neurite outgrowth underlies the wiring of the nervous system during development and regeneration. Despite a significant body of research, the underlying cytoskeletal mechanics of growth and guidance are not fully understood, and the relative contributions of individual cytoskeletal processes to neurite growth are controversial. Here, we review the structural organization and biophysical properties of neurons to make a semi-quantitative comparison of the relative contributions of different processes to neurite growth. Read More

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November 2018

Draft Genome Sequence of sp. Strain PB12/4term, a Thermophilic Facultative Anaerobic Bacterium from Bottom Sediments of Lake Baikal, Russia.

Microbiol Resour Announc 2018 Nov 21;7(20). Epub 2018 Nov 21.

Limnological Institute, Siberian Branch of the Russian Academy of Sciences, Irkutsk, Russia.

Here, we report the draft genome sequence of Thermaerobacter sp. strain PB12/4term, a thermophilic facultative anaerobic bacterium from the bottom sediments of Lake Baikal, Russia, associated with the discharge zone of gas-bearing fluids. Read More

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November 2018

Complete Genome Sequence of the Arcobacter skirrowii Type Strain LMG 6621.

Microbiol Resour Announc 2018 Nov 1;7(17). Epub 2018 Nov 1.

Produce Safety and Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Albany, California, USA.

Arcobacter skirrowii is a species of veterinary importance, originally recovered from the feces, aborted fetuses, and preputial fluids of livestock. We present here the whole-genome sequence of the A. skirrowii type strain LMG 6621 (= 449/80 = CCUG 10374), isolated in the United Kingdom from a lamb diarrheal fecal sample. Read More

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November 2018

Essentials of extracellular vesicles: posters on basic and clinical aspects of extracellular vesicles.

J Extracell Vesicles 2018 30;7(1):1548234. Epub 2018 Nov 30.

Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.

The past decade has witnessed an exponential development in the field of extracellular vesicles. Sporadic observations have reached a critical level and the scientific community increasingly recognizes the potential biomedical significance of these subcellular structures present in all body fluids as significant components of the cellular secretome. The Educational Committee of the International Society for Extracellular Vesicles prepared two posters ("Basic aspects of extracellular vesicles" and "Clinical aspects of extracellular vesicles") to provide essential pieces of information on extracellular vesicles at glance for anyone not familiar with the field. Read More

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November 2018

Microbially Mediated Hydrogen Cycling in Deep-Sea Hydrothermal Vents.

Front Microbiol 2018 23;9:2873. Epub 2018 Nov 23.

Geomicrobiology, GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany.

Deep-sea hydrothermal vents may provide one of the largest reservoirs on Earth for hydrogen-oxidizing microorganisms. Depending on the type of geological setting, hydrothermal environments can be considerably enriched in hydrogen (up to millimolar concentrations). As hot, reduced hydrothermal fluids ascend to the seafloor they mix with entrained cold, oxygenated seawater, forming thermal and chemical gradients along their fluid pathways. Read More

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November 2018