Generation and selection of naïve fab library for parasitic antigen: Anti-BmSXP antibodies for lymphatic filariasis.
- Noorsharmimi Omar,
- Nurul Hamizah Hamidon,
- Muhammad Hafiznur Yunus,
- Rahmah Noordin,
- Yee Siew Choong,
- Theam Soon Lim
Biotechnol Appl Biochem 2017 Aug 21. Epub 2017 Aug 21.
Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia.
Phage display has been applied successfully as a tool for the generation of monoclonal antibodies. Naive antibody libraries are unique as they are able to overcome several limitations associated with conventional monoclonal antibody generation methods like the hybridoma technology. Here, we performed an in vitro selection and generation of Fab antibodies against BmSXP, a recombinant antigen for the detection of lymphatic filariasis. Read More