182,358 results match your criteria Factor II


Reelin dorsal horn neurons co-express Lmx1b and are mispositioned in disabled-1 mutant mice.

Eur J Neurosci 2020 Jun 3. Epub 2020 Jun 3.

Department of Integrative Biology and Physiology, UCLA, Terasaki Life Science Building, 610 Charles E. Young Drive East, Los Angeles, CA, 90095-7239, USA.

Mice missing either Reelin or Disabled-1 (Dab1) exhibit dorsal horn neuronal positioning errors and display heat hypersensitivity and mechanical insensitivity. Reelin binds its receptors, Apolipoprotein E receptor 2 and Very-low-density lipoprotein receptor, leading to the recruitment and phosphorylation of Dab1 and activation of downstream pathways that regulate neuronal migration. Previously, we reported that 70% of Dab1 laminae I-II neurons co-expressed LIM homeobox transcription factor 1 beta (Lmx1b). Read More

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http://dx.doi.org/10.1111/ejn.14847DOI Listing

Suppressed microRNA-195-5p expression in mycosis fungoides promotes tumor cell proliferation.

Exp Dermatol 2020 Jun 3. Epub 2020 Jun 3.

Department of Dermatology, Aarhus University Hospital, Aarhus, Denmark.

Background: Several cancers, including mycosis fungoides (MF), have reported dysregulation of miR-195-5p. miR-195-5p plays a role in cell cycle regulation in several malignant diseases.

Objectives: This study aimed to investigate: i) the expression level of miR-195-5p in lesional MF skin biopsies and II) the potential regulatory roles of miR-195-5p in MF. Read More

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http://dx.doi.org/10.1111/exd.14124DOI Listing

New insights into risk factors for transplant-associated thrombotic microangiopathy in pediatric HSCT.

Blood Adv 2020 Jun;4(11):2418-2429

Department of Blood and Marrow Transplantation, Great Ormond Street (GOS) Hospital for Children NHS Foundation Trust, University College London GOS Institute of Child Health, and NIHR GOSH BRC, London, United Kingdom.

This study aimed to identify a risk profile for development of transplant-associated thrombotic microangiopathy (TA-TMA) in children undergoing hematopoietic stem cell transplantation (HSCT). Between 2013 and 2016, 439 children underwent 474 HSCTs at 2 supraregional United Kingdom centers. At a median of 153 days post-HSCT, TA-TMA occurred among 25 of 441 evaluable cases (5. Read More

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http://dx.doi.org/10.1182/bloodadvances.2019001315DOI Listing

Protein Tyrosine Phosphatase-α Amplifies TGF-β-Dependent Pro-Fibrotic Signaling in Lung Fibroblasts.

Am J Physiol Lung Cell Mol Physiol 2020 Jun 3. Epub 2020 Jun 3.

Academic Affairs, National Jewish Health, United States.

Idiopathic Pulmonary Fibrosis (IPF) is a progressive fibrosing lung disease for which treatment remains suboptimal. Fibrogenic cytokines, including transforming growth factor-β, are central to its pathogenesis. Protein Tyrosine Phosphatase-alpha (PTPα) has emerged as a key regulator of fibrogenic signaling in fibroblasts. Read More

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http://dx.doi.org/10.1152/ajplung.00235.2019DOI Listing

The Effect of Direct Oral Anticoagulants on Antithrombin Activity Testing Is Abolished by DOAC-Stop in Venous Thromboembolism Patients.

Arch Pathol Lab Med 2020 Jun 3. Epub 2020 Jun 3.

From the Institute of Cardiology, Jagiellonian University Medical College and John Paul II Hospital, Kraków, Poland (Drs Ząbczyk and Natorska, Ms Kopytek, and Dr Undas); and the Faculty of Health Sciences, Jagiellonian University Medical College, Kraków, Poland (Mr Malinowski).

Context.—: Direct oral anticoagulants (DOACs) may cause falsely negative results of antithrombin (AT) deficiency screening.

Objective. Read More

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http://dx.doi.org/10.5858/arpa.2020-0021-OADOI Listing

From Hemophilia to Deep Venous Thrombosis Patient Samples: How to Perform an Easy Coagulometer Validation Process According to Available Guidelines.

Clin Appl Thromb Hemost 2020 Jan-Dec;26:1076029620915512

Laboratory Hemostasis and Thrombosis, Hematology and Hemotherapy Center, University of Campinas-UNICAMP Campinas, São Paulo, Brazil.

Validation protocols for the evaluation of coagulometers are needed to help professionals select the most suitable system for their regular laboratory routines. The objective of this study was to show how high standard protocols for the coagulometer validation process can fit into the daily laboratory routine. For this study, 45 healthy individuals and 112 patient samples were analyzed. Read More

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http://dx.doi.org/10.1177/1076029620915512DOI Listing

Comprehensive analysis of RNA binding motif protein 3 (RBM3) in non-small cell lung cancer.

Cancer Med 2020 Jun 3. Epub 2020 Jun 3.

Department of Clinical Sciences, Division of Oncology and Pathology, Lund University, Lund, Sweden.

Aims: High expression of the RNA-binding motif protein 3 (RBM3) correlates with improved prognosis in several major types of cancer. The aim of the present study was to examine the prognostic value of RBM3 protein and mRNA expression in non-small cell lung cancer (NSCLC).

Methods And Results: Immunohistochemical expression of RBM3 was evaluated in surgically treated NSCLC from two independent patient populations (n = 213 and n = 306). Read More

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http://dx.doi.org/10.1002/cam4.3149DOI Listing

The role of Angiology and Vascular Surgery in the COVID-19 pandemic.

Rev Col Bras Cir 2020 1;47:e20202595. Epub 2020 Jun 1.

Laboratory of Physiology and Microcirculation, University of the State of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.

The New Coronavirus Epidemic (2019-nCoV), discovered in the city of Wuhan, China, in December 2019, presents mainly with pulmonary pneumonia that is preceded by fever, cough and myalgia. However, as the disease spread globally and the number of hospitalizations increased exponentially, it was noted that most serious patients hospitalized by COVID-19 have laboratory changes worthy of attention, such as lymphopenia, neutrophilia, increased time of prothrombin and increased levels of D-dimer. Due to these changes proving to be crucial for the mortality and morbidity rates in this subset of infected people, several studies focusing on the pathophysiology, mainly hematological, of the disease appear every day. Read More

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http://dx.doi.org/10.1590/0100-6991e-20202595DOI Listing

Maternal separation changes maternal care, anxiety-like behaviour and expression of paraventricular oxytocin and corticotrophin-releasing factor immunoreactivity in lactating rats.

J Neuroendocrinol 2020 May 7:e12861. Epub 2020 May 7.

Department of Psychology, Macquarie University, North Ryde, NSW, Australia.

The early postnatal period is a time of tremendous change for the dam and her offspring. During this time, environmental insults such as repeated stress exposure can have detrimental effects. In research that has focused on the effect of postnatal stress exposure on the dams, conflicting changes in maternal care and anxiety-like behaviour have been reported. Read More

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http://dx.doi.org/10.1111/jne.12861DOI Listing

Data on genetic polymorphism of flax ( L.) pathogenic fungi of , and genera.

Data Brief 2020 Aug 15;31:105710. Epub 2020 May 15.

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.

Being a valuable agricultural plant, flax ( L.) is used for oil and fiber production. However, the cultivation of this agriculture faces an urgent problem of flax susceptibility to fungal diseases. Read More

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http://dx.doi.org/10.1016/j.dib.2020.105710DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256288PMC

High BMPR2 expression leads to enhanced SMAD1/5/8 signalling and GDF6 responsiveness in human adipose-derived stem cells: implications for stem cell therapies for intervertebral disc degeneration.

J Tissue Eng 2020 Jan-Dec;11:2041731420919334. Epub 2020 May 18.

Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Science Centre, Oxford Road, Manchester, UK.

Stem cell-based regenerative strategies are promising for intervertebral disc degeneration. Stimulation of bone-marrow- and adipose-derived multipotent stem cells with recombinant human growth differentiation factor 6 (rhGDF6) promotes anabolic nucleus pulposus like phenotypes. In comparison to mesenchymal stem cells, adipose-derived multipotent stem cells exhibit greater NP-marker gene expression and proteoglycan-rich matrix production. Read More

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http://dx.doi.org/10.1177/2041731420919334DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238299PMC

Paeoniflorin regulates the hypothalamic-pituitary-adrenal axis negative feedback in a rat model of post-traumatic stress disorder.

Iran J Basic Med Sci 2020 Apr;23(4):439-448

The Research Centre of Basic Integrative Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.

Objectives: To investigate the effects of paeoniflorin (PEF) on the hypothalamic-pituitary-adrenal (HPA) axis feedback function of post-traumatic stress disorder (PTSD).

Materials And Methods: Single-prolonged stress (SPS) was used to establish a PTSD-like rat model. The contents of plasma corticosterone (CORT), adrenocorticotropin hormone (ACTH) and corticotropin-releasing hormone (CRH) were measured by ELISA. Read More

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http://dx.doi.org/10.22038/ijbms.2020.41214.9738DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7239430PMC

SALL4 promotes gastric cancer progression via hexokinase II mediated glycolysis.

Cancer Cell Int 2020 24;20:188. Epub 2020 May 24.

Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013 China.

Background: The stem cell factor SALL4 is reactivated in human cancers. SALL4 plays diverse roles in tumor growth, metastasis, and drug resistance, but its role in tumor metabolism has not been well characterized.

Methods: The glycolytic levels of gastric cancer cells were detected by glucose uptake, lactate production, lactate dehydrogenase activity, ATP level, and hexokinase activity. Read More

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http://dx.doi.org/10.1186/s12935-020-01275-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247129PMC

Changes in serum inflammatory factor interleukin-6 levels and pathology of carotid vessel walls of rats with chronic periodontitis and diabetes mellitus after the periodontal intervention.

Saudi J Biol Sci 2020 Jun 23;27(6):1679-1684. Epub 2020 Apr 23.

Department of Stomatology, Affiliated Hospital of Hangzhou Normal University, No 126, Wenzhou Road, Hangzhou 310015, China.

Objective: The effects of the periodontal intervention on rats with type-II diabetes mellitus (T2DM) and chronic periodontitis (CP) were explored through observing the changes in carotid artery pathology and interleukin-6 (IL-6) levels.

Methods: The rats were randomly divided into 5 groups, i.e. Read More

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http://dx.doi.org/10.1016/j.sjbs.2020.04.029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7254042PMC

Relationship between the core features of Tellenbach's Typus Melancholicus and unipolar depressive disorders: a cross-sectional study.

Riv Psichiatr 2020 May-Jun;55(3):191-194

Department of Human Neurosciences, Faculty of Medicine and Dentistry, Sapienza University of Rome, Italy.

Purpose: One of the most consistent models investigating the relationship between premorbid personality and depression was described by Tellenbach. According to this model, concern with orderliness, conscientiousness, hyper/hetereonomia and intolerance of ambiguity are the core features of Typus Melancholicus (TM). Previous studies showed a relationship between unipolar depression and TM. Read More

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http://dx.doi.org/10.1708/3382.33576DOI Listing

Immune phenotype of tumor microenvironment predicts response to bevacizumab in neoadjuvant treatment of ER positive breast cancer.

Int J Cancer 2020 Jun 3. Epub 2020 Jun 3.

Dept of Oncology, Oslo University Hospital, Oslo, Norway.

Antiangiogenic drugs are potentially a useful supplement to neoadjuvant chemotherapy for a subgroup of patients with HER2 (Human Epidermal Growth Factor receptor 2) negative breast cancer, but reliable biomarkers for improved response are lacking. Here, we report on a randomized phase II clinical trial to study the added effect of bevacizumab in neoadjuvant chemotherapy with FEC100 (5-fluorouracil, epirubicin and cyclophosphamide) and taxanes (n = 132 patients). Gene expression from the tumors was obtained before neoadjuvant treatment, and treatment response was evaluated by Residual Cancer Burden (RCB) at time of surgery. Read More

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http://dx.doi.org/10.1002/ijc.33108DOI Listing

[Hyponatremia in older persons (part I)-keep diagnosis simple : How to detect tricks and avoid pitfalls].

Z Gerontol Geriatr 2020 Jun 2. Epub 2020 Jun 2.

Universitätsklinik für Geriatrie, Medizinische Klinik 2 - Schwerpunkt Geriatrie, Klinikum Nürnberg, Paracelsus Medizinische Privatuniversität, Campus Nürnberg, Nürnberg, Deutschland.

Old age is a strong independent risk factor for hyponatremia. Dizziness, fatigue, reduced vigilance, cognitive impairment, gait deficits, nausea, vomiting, headache, falls, osteoporosis and fractures, coma and seizures are more frequent and severe than in middle-aged patients. Hyponatremia is mainly caused by the syndrome of inappropriate antidiuretic hormone (SIADH) secretion and also including drugs. Read More

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http://dx.doi.org/10.1007/s00391-020-01736-zDOI Listing

Newly developed stroke in patients admitted to non-neurological intensive care units.

J Neurol 2020 Jun 2. Epub 2020 Jun 2.

Department of Neurology, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul, 05505, Republic of Korea.

Background: Little is known about newly developed stroke in patients admitted to the intensive care unit (ICU).

Objective: This study aimed to investigate characteristics and outcomes of newly developed stroke in patients admitted to the non-neurological intensive care units (ICU-onset stroke, IOS).

Methods: A consecutive series of adult patients who were admitted to the non-neurological ICU were included in this study. Read More

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http://dx.doi.org/10.1007/s00415-020-09955-5DOI Listing

Phase 2 study of cabazitaxel as second-line treatment in patients with HER2-negative metastatic breast cancer previously treated with taxanes-a Hellenic Cooperative Oncology Group (HeCOG) Trial.

Br J Cancer 2020 Jun 3. Epub 2020 Jun 3.

Aristotle University of Thessaloniki, Thessaloniki, Greece.

Background: Cabazitaxel is a novel taxane that might be active in breast cancer resistant to first-generation taxanes.

Methods: The purpose of the current multicentre phase II trial was to evaluate the activity and safety of cabazitaxel, as second-line treatment, in patients with human epidermal growth factor receptor 2 (HER2)-negative metastatic breast cancer (MBC) previously treated with taxanes. The primary endpoint was objective response rate (ORR). Read More

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http://dx.doi.org/10.1038/s41416-020-0909-4DOI Listing

Two-Step Mechanism of Cyclin B Degradation Initiated by Proteolytic Cleavage with the 26 S Proteasome in Fish.

Sci Rep 2020 Jun 2;10(1):8924. Epub 2020 Jun 2.

CREST Research Project, Japan Science and Technology Corporation, Shizuoka, Japan.

To complete meiosis II, cyclin B is degraded in a short period by the inactivation of M-phase promoting factor (MPF). Previously, we showed that the destruction of cyclin B was initiated by the ubiquitin-independent proteolytic activity of the 26 S proteasome through an initial cut in the N-terminus of cyclin (at K57 in the case of goldfish cyclin B). We hypothesized that this cut allows cyclin to be ubiquitinated for further destruction by the ubiquitin-dependent proteolytic pathway, which leads to MPF inactivation. Read More

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http://dx.doi.org/10.1038/s41598-020-65009-wDOI Listing

Relationship between Energy Dosage and Apoptotic Cell Death by Modulated Electro-Hyperthermia.

Sci Rep 2020 Jun 2;10(1):8936. Epub 2020 Jun 2.

Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, Hsinchu, Taiwan.

Modulated electro-hyperthermia (mEHT) is a form of mild hyperthermia (HT) used for cancer treatment. The principle utility of HT is the ability not only to increase cell temperature, but also to increase blood flow and associated pO to the microenvironment. While investigational evidence has shown the unique ability of mEHT to elicit apoptosis in cancer cells, in vivo and in vitro, the same trait has not been observed with conventional HT. Read More

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http://dx.doi.org/10.1038/s41598-020-65823-2DOI Listing

Association between nutrition support and acute gastrointestinal injury in critically ill patients during the first 72 hours.

Clin Nutr 2020 May 18. Epub 2020 May 18.

Intensive Care Unit, First Hospital of Jilin University, Changchun, China. Electronic address:

Background & Aims: The impact of nutrition support on patients with acute gastrointestinal injury (AGI) has not been fully determined. This study aimed to 1) investigate the relationship between nutrition support and AGI, as well as nutrition support and prognosis in critically ill AGI patients and 2) evaluate the prognostic benefits of nutrition support in different severity categories of AGI patients.

Methods: This prospective study included 379 patients in whom AGI occurred in the first 72 h after admission from 12 teaching hospitals in China. Read More

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http://dx.doi.org/10.1016/j.clnu.2020.05.011DOI Listing

Detection and dynamics of circulating tumor cells in patients with high-risk prostate cancer treated with radiotherapy and hormones: a prospective phase II study.

Radiat Oncol 2020 Jun 1;15(1):137. Epub 2020 Jun 1.

Methodology Unit, Health Research Institute of Hospital Universitario de La Princesa, Madrid, Spain.

Background: Circulating tumor cells (CTCs) are an established prognostic marker in castration-resistant prostate cancer but have received little attention in localized high-risk disease. We studied the detection rate of CTCs in patients with high-risk prostate cancer before and after androgen deprivation therapy and radiotherapy to assess its value as a prognostic and monitoring marker.

Patients And Methods: We performed a prospective analysis of CTCs in the peripheral blood of 65 treatment-naïve patients with high-risk prostate cancer. Read More

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http://dx.doi.org/10.1186/s13014-020-01577-5DOI Listing

Histologic subtyping affecting outcome of triple negative breast cancer: a large Sardinian population-based analysis.

BMC Cancer 2020 Jun 2;20(1):491. Epub 2020 Jun 2.

Department of Medical, Surgical and Experimental Sciences, University of Sassari, Via P. Manzella, 4, 07100, Sassari, Italy.

Background: Triple Negative breast cancer (TNBC) includes a heterogeneous group of tumors with different clinico-pathological features, molecular alterations and treatment responsivity. Our aim was to evaluate the clinico-pathological heterogeneity and prognostic significance of TNBC histologic variants, comparing "special types" to high-grade invasive breast carcinomas of no special type (IBC-NST).

Methods: This study was performed on data obtained from TNBC Database, including pathological features and clinical records of 1009 TNBCs patients diagnosed between 1994 and 2015 in the four most important Oncology Units located in different hospitals in Sardinia, Italy. Read More

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http://dx.doi.org/10.1186/s12885-020-06998-9DOI Listing

Imaging-Based Prediction of Molecular Therapy Targets in NSCLC by Radiogenomics and AI Approaches: A Systematic Review.

Diagnostics (Basel) 2020 May 30;10(6). Epub 2020 May 30.

Humanitas University, Pieve Emanuele, Milan 20090, Italy.

The objective of this systematic review was to analyze the current state of the art of imaging-derived biomarkers predictive of genetic alterations and immunotherapy targets in lung cancer. We included original research studies reporting the development and validation of imaging feature-based models. The overall quality, the standard of reporting and the advancements towards clinical practice were assessed. Read More

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http://dx.doi.org/10.3390/diagnostics10060359DOI Listing

Exercise Attenuates the Transition from Fatty Liver to Steatohepatitis and Reduces Tumor Formation in Mice.

Cancers (Basel) 2020 May 29;12(6). Epub 2020 May 29.

Hepatology, Department for BioMedical Research, University of Bern, Bern 3008, Switzerland.

Non-alcoholic fatty liver disease (NAFLD) leads to steatohepatitis (NASH), fibrosis, and hepatocellular carcinoma. For sedentary patients, lifestyle interventions combining exercise and dietary changes are a cornerstone of treatment. However, the benefit of exercise alone when dietary changes have failed is uncertain. Read More

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http://dx.doi.org/10.3390/cancers12061407DOI Listing

Axial Compressive Behaviour of Square through-Beam Joints between CFST Columns and RC Beams with Multi-Layers of Steel Meshes.

Materials (Basel) 2020 May 29;13(11). Epub 2020 May 29.

School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.

The axial compressive behaviour of an innovative type of square concrete filled steel tube (CFST) column to reinforced concrete (RC) beam joint was experimentally investigated in this paper. The innovative joint was designed such that (i) the steel tubes of the CFST columns were completely interrupted in the joint region, (ii) the longitudinal reinforcements from the RC beams could easily pass through the joint area and (iii) a reinforcement cage, including a series of reinforcement meshes and radial stirrups, was arranged in the joint area to strengthen the mechanical performance of the joint. A twostage experimental study was conducted to investigate the behaviour of the innovative joint under axial compression loads, where the first stage of the tests included three fullscale innovative joint specimens subjected to axial compression to assess the feasibility of the joint detailing and propose measures to further improve its axial compressive behaviour, and the second stage of the tests involved 14 innovative joint specimens with the improved detailing to study the effect of the geometric size of the joint, concrete strength and volume ratio of the steel meshes on the bearing strengths of the joints. Read More

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http://dx.doi.org/10.3390/ma13112482DOI Listing

Hepatitis B Virus DNA is a Substrate for the cGAS/STING Pathway but is not Sensed in Infected Hepatocytes.

Viruses 2020 May 29;12(6). Epub 2020 May 29.

Department of Infectious Diseases, Molecular Virology, University Hospital Heidelberg, 69120 Heidelberg, Germany.

Hepatitis B virus HBV) chronic infection is a critical risk factor for hepatocellular carcinoma. The innate immune response to HBV infection is a matter of debate. In particular, viral escape mechanisms are poorly understood. Read More

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http://dx.doi.org/10.3390/v12060592DOI Listing

A Nutritional Supplement (DìLsh) Improves the Inflammatory Cytokines Response, Oxidative Stress Markers and Clinical Signs in Dogs Naturally Infected by .

Animals (Basel) 2020 May 29;10(6). Epub 2020 May 29.

Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Napoli, Italy.

The possibility to associate nutraceuticals, as immune-modulating tools, to the treatment of visceral leishmaniosis is a matter of great interest. In this study, we investigated whether the administration of a nutritional supplement (DìLsh, Dynamopet SRL, Verona, Italy) was able to exert beneficial effects on the inflammatory state and oxidative stress of the dogs naturally infected by Leishmania infantum. To this purpose, specific parameters, namely Tumor Necrosis Factor -alpha (TNF), Interleukin-6 (IL-6), Inteleukin-10 (IL-10), leptin, derivates of Reactive Oxigen Metabolites (d-Roms) and Biological Antioxidant Potential (BAP), as well as the haematological and biochemical profiles of the infected dogs, were investigated upon the treatment with the nutritional supplement and compared with the conventional pharmacological anti- therapy. Read More

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http://dx.doi.org/10.3390/ani10060938DOI Listing

Silica as a morphogenetically active inorganic polymer.

Biomater Sci 2013 Jun 2;1(6):669-678. Epub 2013 Apr 2.

ERC Advanced Grant Research Group at the Institute for Physiological Chemistry, University Medical Center of the Johannes Gutenberg University Mainz, Duesbergweg 6, D-55128 Mainz, Germany.

At present the scaffolds used for bioprinting of cells do not elicit morphogenetic responses in the cells. In the present study we approached a solution by studying the effect of an inorganic silica supplement added to an Na-alginate matrix. Bone- and osteoblast-like SaOS-2 cells were embedded into this organic polymeric matrix which was additionally enriched with 400 μM prehydrolyzed TEOS [tetra-ethoxy-silane], a source of ortho-silicate. Read More

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http://dx.doi.org/10.1039/c3bm00001jDOI Listing

Biologically inspired membrane design with a heparin-like interface: prolonged blood coagulation, inhibited complement activation, and bio-artificial liver related cell proliferation.

Biomater Sci 2014 Jan 17;2(1):98-109. Epub 2013 Sep 17.

College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, People's Republic of China.

In the present work, inspired by the chemical structure of heparin molecules, we designed a polyethersulfone (PES) membrane with a heparin-like surface for the first time by physically blending sulfonated polyethersulfone (SPES), carboxylic polyethersulfone (CPES), and PES at rational ratios. Evaporation and phase-inversion membranes of PES/CPES/SPES were prepared by evaporating the solvent in a vacuum oven, and by a liquid-liquid phase separation technique, respectively. Scanning electron microscopy (SEM) images revealed that the structures of the PES/CPES/SPES membranes were dependent on the proportions of the additives and no obvious phase separation was detected. Read More

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http://dx.doi.org/10.1039/c3bm60165jDOI Listing
January 2014

On the induction of injury in tomato under continuous light: circadian asynchrony as the main triggering factor.

Funct Plant Biol 2017 Jun;44(6):597-611

Horticulture and Product Physiology, Wageningen University, PO Box 630, 6700 AP Wageningen, The Netherlands.

Unlike other species, when tomato plants (Solanum lycopersicum L.) are deprived of at least 8h of darkness per day, they develop a potentially lethal injury. In an effort to understand why continuous light (CL) is injurious to tomato, we tested five factors, which potentially could be responsible for triggering the injury in CL-grown tomato: (i) differences in the light spectral distribution between sunlight and artificial light, (ii) continuous light signalling, (iii) continuous supply of light for photosynthesis, (iv) continuous photo-oxidative pressure and (v) circadian asynchrony - a mismatch between the internal circadian clock frequency and the external light/dark cycles. Read More

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http://dx.doi.org/10.1071/FP16285DOI Listing

Drivers of pesticide resistance in freshwater amphipods.

Sci Total Environ 2020 May 8;735:139264. Epub 2020 May 8.

UFZ, Helmholtz Centre for Environmental Research, Department of System-Ecotoxicology, Permoserstraße 15, 04318 Leipzig, Germany; RWTH Aachen University, Institute for Environmental Research (Biology V), Worringerweg 1, 52074 Aachen, Germany. Electronic address:

Aquatic invertebrates exposed to pesticides may develop pesticide resistance. Based on a meta-analysis we revealed environmental factors driving the magnitude of resistance in the freshwater amphipod Gammarus pulex in the field. We showed that (i) insecticide tolerance of G. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2020.139264DOI Listing

Maternal alcohol consumption during pregnancy associates with bone fractures in early childhood. A birth-cohort study of 6718 participants.

Bone 2020 May 30:115462. Epub 2020 May 30.

Department of Pediatric Surgery and Orthopaedics, Oulu University Hospital, PEDEGO-research unit and Medical Research Center, University of Oulu, Oulu, Finland.

Fractures are common injuries in children, but their underlying biological and environmental risk factors are not well known. Maternal alcohol consumption during pregnancy is a known risk factor for bone malformations and impaired growth, in connection with Fetal Alcohol Spectrum Disorders (FASD). There is evidence that even lower doses of alcohol than what is needed for FASD can cause changes in the developing bone. Read More

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http://dx.doi.org/10.1016/j.bone.2020.115462DOI Listing

Conformational activation of ribosome recycling by intra- and inter-molecular dynamics of RRF.

Int J Biol Macromol 2020 May 30. Epub 2020 May 30.

Key Laboratory of RNA Biology, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Ribosome recycling is the final step of the cyclic process of translation, where the post-termination complex (PoTC) is disassembled by the concerted action of ribosome recycling factor (RRF) and elongation factor G (EF-G) in the sub-second time range. Since, however, both the RRF and PoTC display highly dynamic action during this process, it is difficult to assess the molecular details of the interactions between the factors and the ribosome that are essential for rapid subunit separation. Here we characterized the molecular dynamics of RRF and PoTC by combined use of molecular dynamics simulations, single molecule fluorescence detection and single-particle cryo-EM analysis, with time resolutions in the sub-millisecond to minute range. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2020.05.254DOI Listing

Cyclic AMP represses pathological MEF2 activation by myocyte-specific hypo-phosphorylation of HDAC5.

J Mol Cell Cardiol 2020 May 30. Epub 2020 May 30.

Institute of Experimental Cardiology, University of Heidelberg, Heidelberg, Germany; DZHK (German Centre for Cardiovascular Research), partner site Heidelberg/Mannheim, Germany. Electronic address:

Class IIa histone deacetylases (HDACs) critically regulate cardiac function through the repression of the activity of myocyte enhancer factor 2 (MEF2)-dependent gene programs. Protein kinase D (PKD) and Ca/Calmodulin-dependent kinase II (CaMKII) activate MEF2 by phosphorylating distinct HDAC isoforms and thereby creating 14-3-3 binding sites for nucleo-cytoplasmic shuttling. Recently, it has been shown that this process is counteracted by cyclic AMP (cAMP)-dependent signaling. Read More

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http://dx.doi.org/10.1016/j.yjmcc.2020.05.018DOI Listing

Effects of a single dose of vitamin D in septic children: a randomized, double-blinded, controlled trial.

J Int Med Res 2020 Jun;48(6):300060520926890

Department of Pediatrics, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, Henan, China.

Objective: To assess the effects of a single dose of vitamin D on 25-hydroxyvitamin D (25OHD) levels and clinical outcomes in children with vitamin D deficiency (VDD) and sepsis.

Methods: In this randomized, controlled trial, eligible children with VDD and sepsis were assigned to receive one dose of 150,000 IU of cholecalciferol or placebo. Serum concentrations of 25OHD, angiotensin-II (Ang-II), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) were assessed at baseline and 8 days after treatment. Read More

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http://dx.doi.org/10.1177/0300060520926890DOI Listing

Vascular Endothelial Growth Factor (VEGF) as a Vital Target for Brain Inflammation during the COVID-19 Outbreak.

ACS Chem Neurosci 2020 Jun 2. Epub 2020 Jun 2.

Department of Clinical Pharmacology, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha 410008, P. R. China.

The coronavirus disease 19 (COVID-19) pandemic has brought a great threat to global public health. Currently, mounting evidence has shown the occurrence of neurological symptoms in patients with COVID-19. However, the detailed mechanism by which the SARS-CoV-2 attacks the brain is not well characterized. Read More

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http://dx.doi.org/10.1021/acschemneuro.0c00294DOI Listing

TGF-β1-induced expression of collagen type II and ACAN is regulated by 4E-BP1, a repressor of translation.

FASEB J 2020 Jun 2. Epub 2020 Jun 2.

Division of Rheumatology, Department of Internal Medicine, Hallym University Sacred Heart Hospital, Kyunggi, Korea.

Eukaryotic initiation factor 4E (eIF4E)-binding protein 1 (4E-BP1) binds eIF4E and represses protein translation by displacing it from the mRNA. In this study, we investigated the influence of 4E-BP1 translational apparatus on the regulation of transforming growth factor-beta 1 (TGF-β1)-induced anabolic signaling in chondrocytes. The level of 4E-BP1 expression was significantly higher in human OA cartilage than normal cartilage. Read More

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http://dx.doi.org/10.1096/fj.201903003RDOI Listing

The potential of microRNA-126 in predicting disease risk, mortality of sepsis, and its correlation with inflammation and sepsis severity.

J Clin Lab Anal 2020 Jun 2:e23408. Epub 2020 Jun 2.

Department of Emergency, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, China.

Background: MicroRNA-126 (miR-126) has been investigated in autoimmune diseases and organ failures, whereas its implication in sepsis is rarely reported. Our study initially explored the value of miR-126 in diagnosing sepsis and predicting disease severity, degree of inflammation, and mortality.

Methods: Totally, 208 sepsis patients and 210 healthy controls were enrolled; then, their plasma samples were collected for detecting circulating miR-126 by quantitative polymerase chain reaction. Read More

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http://dx.doi.org/10.1002/jcla.23408DOI Listing

Penetrated inferior vena cava filter retrieved by open surgery with deep hypothermic circulatory arrest.

J Card Surg 2020 Jun 2. Epub 2020 Jun 2.

Department of Cardiology, Instituto de Cardiologia do Distrito Federal, Brasilia, Brazil.

Background And Aim: Complications of inferior vena cava filters are relatively common, and they vary according to different filter types and designs. We aim to present a case of penetrated inferior vena cava filter into the liver.

Methods: Case report. Read More

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http://dx.doi.org/10.1111/jocs.14677DOI Listing

Screening for potential nuclear substrates for the plant cell death suppressor kinase Adi3 using peptide microarrays.

PLoS One 2020 2;15(6):e0234011. Epub 2020 Jun 2.

Department of Biochemistry & Biophysics, Texas A&M University, College Station, Texas, United States of America.

The tomato AGC protein kinase Adi3 is a Ser/Thr kinase that functions as a negative regulator of programmed cell death through cell death suppression (CDS) activity in the nucleus. In this study, to understand the mechanism of Adi3 CDS, peptide microarrays containing random Ser- and Thr-peptide phosphorylation substrates were used to screen for downstream phosphorylation substrates. In the microarray phosphorylation assay, Adi3 showed promiscuous kinase activity more toward Ser-peptides compared to Thr-peptides, and a preference for aromatic and cyclic amino acids on both Ser- and Thr-peptides was seen. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0234011PLOS

Protein QTL analysis of IGF-I and its binding proteins provides insights into growth biology.

Hum Mol Genet 2020 Jun 1. Epub 2020 Jun 1.

Division of Endocrinology, Children's National Hospital, Washington, DC 20010, USA.

The growth hormone and insulin-like growth factor (IGF) system is integral to human growth. Genome-wide association studies (GWAS) have identified variants associated with height and located near the genes in this pathway. However, mechanisms underlying these genetic associations are not understood. Read More

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http://dx.doi.org/10.1093/hmg/ddaa103DOI Listing

Stem Bromelain Proteolytic Machinery: Study of the Effects of its Components on Fibrin (ogen) and Blood Coagulation.

Protein Pept Lett 2020 May 25. Epub 2020 May 25.

Plant Proteins Research Center (CIProVe), Department of Biological Sciences, Faculty of Exact Sciences, National University of La Plata - CICPBA, La Plata. Argentina.

Background: Antiplatelet, anticoagulant and fibrinolytic activities of stem bromelain (EC 3.4.22. Read More

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http://dx.doi.org/10.2174/0929866527666200525163622DOI Listing

Optimized immobilization strategy for dirhodium (II) carboxylate catalysts for C-H functionalization and their implementation in a packed bed flow reactor.

Angew Chem Int Ed Engl 2020 Jun 1. Epub 2020 Jun 1.

Georgia Institute of Technology, Sch. of Chem. and Biomol.Engin., 311 Ferst Dr Dr, 30332, Atlanta, UNITED STATES.

Herein we demonstrate a sustainable, scalable packed bed flow reactor capable of achieving highly regio- and stereoselective C-H functionalization reactions using a newly developed Rh 2 ( S -2-Cl-5-CF 3 TPCP) 4 catalyst. To optimize the immobilized dirhodium catalyst employed in the flow reactor, we systematically study both (i) the effects of ligand immobilization position, demonstrating the critical factor that the catalyst-support attachment location can have on the catalyst performance, and (ii) silica support mesopore length, demonstrating that decreasing diffusional limitations leads to increased accessibility of the active site and higher catalyst turnover frequency. We employ the immobilized dirhodium catalyst in a simple packed bed flow reactor achieving comparable yields and levels of enantioselectivity to the homogeneous catalyst employed in batch and maintain this performance over ten catalyst recycles. Read More

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http://dx.doi.org/10.1002/anie.202005381DOI Listing

Association between benign paroxysmal positional vertigo and osteoporosis: two nested case-control studies.

Osteoporos Int 2020 Jun 1. Epub 2020 Jun 1.

Department of Otorhinolaryngology-Head & Neck Surgery, Hallym University Sacred Heart Hospital, Hallym University College of Medicine, 22, Gwanpyeong-ro 170beon-gil, Dongan-gu, Anyang-si, Gyeonggi-do, 14068, Republic of Korea.

Benign paroxysmal positional vertigo (BPPV) was related to a 1.28 times higher risk of osteoporosis. In addition, osteoporosis was associated with a 1. Read More

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http://dx.doi.org/10.1007/s00198-020-05478-xDOI Listing

A genome-wide gain-of-function screen identifies CDKN2C as a HBV host factor.

Nat Commun 2020 Jun 1;11(1):2707. Epub 2020 Jun 1.

Université de Strasbourg, Inserm, Institut de Recherche sur les Maladies Virales et Hépatiques UMR_S1110, F-67000, Strasbourg, France.

Chronic HBV infection is a major cause of liver disease and cancer worldwide. Approaches for cure are lacking, and the knowledge of virus-host interactions is still limited. Here, we perform a genome-wide gain-of-function screen using a poorly permissive hepatoma cell line to uncover host factors enhancing HBV infection. Read More

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http://dx.doi.org/10.1038/s41467-020-16517-wDOI Listing

CAMK2A supported tumor initiating cells of lung adenocarcinoma by upregulating SOX2 through EZH2 phosphorylation.

Cell Death Dis 2020 Jun 1;11(6):410. Epub 2020 Jun 1.

Department of Pathology, The University of Hong Kong, Hong Kong, Hong Kong.

Tumor initiating cells (TIC) of lung cancer are mainly induced by stress-related plasticity. Calcium/Calmodulin dependent protein kinase II alpha (CAMK2A) is a key calcium signaling molecule activated by exogenous and endogenous stimuli with effects on multiple cell functions but little is known about its role on TIC. In human lung adenocarcinomas (AD), CAMK2A was aberrantly activated in a proportion of cases and was an independent risk factor predicting shorter survivals. Read More

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http://dx.doi.org/10.1038/s41419-020-2553-6DOI Listing

Uterine disorders affecting female fertility: what are the molecular functions altered in endometrium?

Fertil Steril 2020 Jun;113(6):1261-1274

Department of Genomic and Systems Reproductive Medicine, IVI-RMA (Instituto Valenciano de Infertilidad, Reproductive Medicine Associates) IVI Foundation, Valencia, Spain; Instituto de Investigación Sanitaria INCLIVA, University of Valencia, Valencia, Spain. Electronic address:

Objective: To determine the molecular functions of genes exhibiting altered expression in the endometrium of women with uterine disorders affecting fertility.

Design: Retrospective analysis integrating case and control data from multiple cohorts with endometrium gene expression in women with uterine disorders.

Setting: Infertility research department affiliated with a university hospital. Read More

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http://dx.doi.org/10.1016/j.fertnstert.2020.01.025DOI Listing

L. Dry Extracts Ameliorate Adipocyte Differentiation of 3T3-L1 Cells Exposed to TNFα by Down-Regulating Expression.

Nutrients 2020 May 28;12(6). Epub 2020 May 28.

URT Genomics of Diabetes, Institute of Experimental Endocrinology and Oncology, National Research Council, 80131 Naples, Italy.

L. dry extracts (de) improve adipogenesis in vitro. These effects are dependent from an early modulation of CCAAT/enhancer-binding protein beta () expression and cyclic Adenosine Monophosphate (cAMP) response element-binding protein () activation. Read More

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http://dx.doi.org/10.3390/nu12061587DOI Listing