12,298 results match your criteria FEMS microbiology letters[Journal]


Characteristics, transcriptional patterns and possible physiological significance of glycoside hydrolase family 16 members in Coprinopsis cinerea.

FEMS Microbiol Lett 2019 Apr 20. Epub 2019 Apr 20.

Jiangsu Key Laboratory for Microbes and Microbial Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Science, Nanjing Normal University, Nanjing 210023, PR China.

The glycoside hydrolase (GH) 16 family of Coprinopsis cinerea includes fifteen members distributed in four subgroups (A1, A2, B and D) by phylogenetic analysis. The expression patterns match well with the requirement of wall-softening in the germination of basidiospores, hyphal growth and branching, primordium formation, stipe elongation, pileus expansion and autolysis. Remarkably, expression levels of different GH16 members varied with different morphogenetic events. Read More

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http://dx.doi.org/10.1093/femsle/fnz083DOI Listing

In silico prediction and characterisation of secondary metabolite clusters in the plant pathogenic fungus Verticillium dahliae.

FEMS Microbiol Lett 2019 Apr 20. Epub 2019 Apr 20.

Laboratory of Phytopathology, Wageningen University & Research, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.

Fungi are renowned producers of natural compounds, also known as secondary metabolites (SMs) that display a wide array of biological activities. Typically, the genes that are involved in the biosynthesis of SMs are located in close proximity to each other in so-called secondary metabolite clusters (SMCs). Many plant-pathogenic fungi secrete SMs during infection in order to promote disease establishment, for instance as cytocoxic compounds. Read More

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http://dx.doi.org/10.1093/femsle/fnz081DOI Listing

The Acidaminococcus sp. Cas12a nuclease recognizes GTTV and GCTV as non-canonical PAMs.

FEMS Microbiol Lett 2019 Apr 20. Epub 2019 Apr 20.

Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695.

The clustered regularly interspaced short palindromic repeat (CRISPR)-associated (Cas) nuclease Acidaminococcus sp. Cas12a (AsCas12a, also known as AsCpf1) has become a popular alternative to Cas9 for genome editing and other applications. AsCas12a has been associated with a TTTV protospacer-adjacent motif (PAM) as part of target recognition. Read More

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http://dx.doi.org/10.1093/femsle/fnz085DOI Listing

Limitations of transcriptome-based prediction of pathogenicity genes in the plant pathogen Leptosphaeria maculans.

FEMS Microbiol Lett 2019 Apr 18. Epub 2019 Apr 18.

School of BioSciences, the University of Melbourne, VIC 3010, Australia.

Identification of pathogenicity determinants in Leptosphaeria maculans, a major cause of disease of oilseed crops, has been a focus of research for many years. A wealth of gene expression information from RNA sequencing promises to illuminate the mechanisms by which the fungus is able to cause blackleg disease. However, to date, no studies have tested the hypothesis that high gene transcript levels during infection correlate with importance to disease progression. Read More

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http://dx.doi.org/10.1093/femsle/fnz080DOI Listing

Predicted highly derived class 1 CRISPR-Cas system in Haloarchaea containing diverged Cas5 and Cas7 homologs but no CRISPR array.

FEMS Microbiol Lett 2019 Apr 17. Epub 2019 Apr 17.

National Center for Biotechnology Information, National Library of Medicine, Bethesda, MD 20894.

Screening of genomic and metagenomic databases for new variants of CRISPR-Cas systems increasingly results in the discovery of derived variants that do not seem to possess the interference capacity and are implicated in functions distinct from adaptive immunity. We describe an extremely derived putative class 1 CRISPR-Cas system that is present in many Halobacteria and consists of distant homologs of the Cas5 and Cas7 protein along with an uncharacterized conserved protein and various nucleases. We hypothesize that, although this system lacks typical CRISPR effectors or a CRISPR array, it functions as a RNA-dependent defense mechanism that, unlike other derived CRISPR-Cas, utilizes alternative nucleases to cleave invader genomes. Read More

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http://dx.doi.org/10.1093/femsle/fnz079DOI Listing

Detection of fecal bacteria and antibiotic resistance genes in drinking water collected from three first nations communities in Manitoba, Canada.

FEMS Microbiol Lett 2019 Apr 13. Epub 2019 Apr 13.

Microbiology.

This study analyzed the microbiological quality of drinking and source water from three First Nations communities in Manitoba, Canada that vary with respect to the source, storage and distribution of drinking water. Community A relies on an aquifer and Community B on a lake as source water to their water treatment plants. Community C does not have a water treatment plant and uses well water. Read More

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http://dx.doi.org/10.1093/femsle/fnz067DOI Listing

Post hatch recovery of a probiotic Enterococcus faecium strain in the yolk sac and intestinal tract of broiler chickens after in ovo injection.

FEMS Microbiol Lett 2019 Apr 13. Epub 2019 Apr 13.

Department of Poultry Science, Mississippi State University, Mississippi State, MS 39762 USA.

Concerns about antibiotic resistant bacteria and their presence in animal products grow and thus alternatives to use of antibiotics in animal production are being investigated. Probiotics have gained increased focus due to improvements in performance, immune health, and pathogen reduction when provided to poultry through feed. These traits may be further improved if probiotics can be provided to the embryo before hatch, before meeting environmental pathogens. Read More

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http://dx.doi.org/10.1093/femsle/fnz078DOI Listing
April 2019
1 Read

Biogeography of the fish pathogen Aeromonas salmonicida inferred by vapA genotyping.

FEMS Microbiol Lett 2019 Apr 12. Epub 2019 Apr 12.

Fish Health Research Group, Norwegian Veterinary Institute, Oslo, Norway.

A recently described typing system based on sequence variation in the virulence array protein (vapA) gene, encoding the A-layer surface protein array, allows unambiguous subtyping of Aeromonas salmonicida. In the present study we compile A-layer typing results from a total of 675 A. salmonicida isolates, recovered over a 59-year period from 50 different fish species in 26 countries. Read More

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https://academic.oup.com/femsle/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/femsle/fnz074DOI Listing
April 2019
5 Reads

Two lytic transglycosylases of Xanthomonas campestris pv. campestris associated with cell separation and type III secretion system respectively.

FEMS Microbiol Lett 2019 Apr 12. Epub 2019 Apr 12.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China.

The lytic transglycosylases (LTs) are important enzymes that degrade peptidoglycan of the bacterial cell wall and affecting many biological functions. We present here that XC_0706 and XC_3001 were annotated as the lytic transglycosylases in Xanthomonas campestris pv. campestris (Xcc). Read More

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http://dx.doi.org/10.1093/femsle/fnz073DOI Listing
April 2019
1 Read

Mapping social media attention in Microbiology: Identifying main topics and actors.

FEMS Microbiol Lett 2019 Apr 12. Epub 2019 Apr 12.

Vicerrectorado de Investigación y Transferencia, Universidad de Granada, Granada, Spain.

This paper aims to map and identify topics of interest within the field of Microbiology and identify the main sources driving such attention. We combine data from Web of Science and Altmetric.com, a platform which retrieves mentions to scientific literature from social media and other non-academic communication outlets. Read More

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http://dx.doi.org/10.1093/femsle/fnz075DOI Listing
April 2019
2 Reads

Validation of an ampicillin selection protocol to enrich for mutants of Listeria monocytogenes unable to replicate on fresh produce.

FEMS Microbiol Lett 2019 Apr 12. Epub 2019 Apr 12.

Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC 27695, USA.

Several outbreaks of listeriosis have implicated fresh produce but genetic factors required for growth of Listeria monocytogenes on produce remain poorly characterized. Based on the fact that β-lactam antibiotics only kill bacterial cells that are growing, we hypothesized that ampicillin selection can enrich for L. monocytogenes mutants unable to grow on produce. Read More

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http://dx.doi.org/10.1093/femsle/fnz076DOI Listing
April 2019
1 Read

The role of the GATA transcription factor AreB in regulation of nitrogen and carbon metabolism in Aspergillus nidulans.

FEMS Microbiol Lett 2019 Apr 2. Epub 2019 Apr 2.

Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, ul. Pawińskiego 5A, 02-106 Warsaw, Poland.

In Aspergillus nidulans, nitrogen and carbon metabolism are under the control of wide-domain regulatory systems, including nitrogen metabolite repression, carbon catabolite repression and the nutrient starvation response. Transcriptomic analysis of the wild type strain grown under different combinations of carbon and nitrogen regimes was performed, to identify differentially regulated genes. Carbon metabolism predominates as the most important regulatory signal but for many genes, both carbon and nitrogen metabolisms coordinate regulation. Read More

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http://dx.doi.org/10.1093/femsle/fnz066DOI Listing
April 2019
6 Reads

A place for taxonomic profiling in the study of the coral prokaryotic microbiome.

FEMS Microbiol Lett 2019 Apr 2. Epub 2019 Apr 2.

Australian Research Council Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, Australia.

The enormous variability in richness, abundance, and diversity of unknown bacterial organisms inhabiting the coral microbiome have challenged our understanding of their functional contribution to coral health. Identifying the attributes of the healthy meta-organism is paramount for contemporary approaches aiming to manipulate dysbiotic stages of the coral microbiome. This review evaluates the current knowledge on the structure and mechanisms driving bacterial communities in the coral microbiome and discusses two topics requiring further research to define the healthy coral microbiome. Read More

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https://academic.oup.com/femsle/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/femsle/fnz063DOI Listing
April 2019
3 Reads

Biofilm development in L. fermentum under shear flow & sequential GIT digestion.

FEMS Microbiol Lett 2019 Mar 30. Epub 2019 Mar 30.

National Probiotic Lab-National Institute for Biotechnology & Genetic Engineering (NIBGE), Jhang Road, Faisalabad 38000, Pakistan.

The objective of this study was to investigate biofilm formation by Lactobacillus fermentum under physiologically relevant shear conditions both in the presence and absence of a food matrix and under simulated conditions of digestion. This was done using batch and flow biofilms of L. fermentum strains under conditions simulating digestion in the human gastrointestinal tract and shear flow using a high throughput platform BioFlux 1000Z system. Read More

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http://dx.doi.org/10.1093/femsle/fnz064DOI Listing
March 2019
1 Read
2.121 Impact Factor

Magnolol protects Saccharomyces cerevisiae antioxidant-deficient mutants from oxidative stress and extends yeast chronological life span.

FEMS Microbiol Lett 2019 Mar 29. Epub 2019 Mar 29.

Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Pondicherry 605014, India.

We investigated the protective effect of a natural polyphenol, magnolol, on Saccharomyces cerevisiae cells under oxidative stress, and during aging. Our results showed the sensitivity of S. cerevisiae antioxidant gene deficient mutants (sod1∆, sod2∆, cta1∆, ctt1∆, gtt2∆ and tsa1∆) against H2O2 and menadione stress was rescued by magnolol as demonstrated in spot and CFU counts. Read More

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http://dx.doi.org/10.1093/femsle/fnz065DOI Listing
March 2019
4 Reads

Utilisation of hydrocarbons and production of surfactants by bacteria isolated from plant leaf surfaces.

FEMS Microbiol Lett 2019 Mar 27. Epub 2019 Mar 27.

School of Biological Sciences, University of Canterbury, Christchurch, New Zealand.

Leaves are covered by a cuticle composed of long (C11-C20) and very-long chain hydrocarbons (> C20), e.g. alkanes, fatty acids, alcohols, aldehydes, ketones and esters. Read More

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http://dx.doi.org/10.1093/femsle/fnz061DOI Listing

Association between diet and rumen microbiota in wild roe deer.

FEMS Microbiol Lett 2019 Mar 27. Epub 2019 Mar 27.

Department of Biotechnology, Faculty of Applied Ecology, Agricultural Sciences & Biotechnology, Inland Norway University of Applied Sciences, 2318 Hamar, Norway.

The association between diet and the rumen microbiota for wild animals remains largely unexplored. Here, we explored this association using a combination of 16S rRNA gene sequencing to determine the prokaryote microbiota, and 18S rRNA gene sequencing to determine the dietary components for wild roe deer. These analyses revealed a wide diversity of dietary components, with overrepresentation of Bacteroidetes for the diet-correlating bacteria. Read More

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http://dx.doi.org/10.1093/femsle/fnz060DOI Listing
March 2019
1 Read

F5Evaluation of commercially available DNA extraction kits for the analysis of the broiler chicken cecal microbiota.

FEMS Microbiol Lett 2019 Mar 27. Epub 2019 Mar 27.

Evonik Nutrition & Care GmbH, Kantstraße 2, 33790 Halle, Germany.

16S amplicon sequencing is a state of the art technology to analyze bacterial communities via microbiome profiling. Choosing an appropriate DNA extraction protocol is crucial for characterizing the microbial community and can be challenging, especially when preliminary knowledge about the sample matrix is scarce. The aim of the present study was to evaluate seven commercial DNA extraction kits suitable for 16S rRNA gene amplicon sequencing of the bacterial community of the chicken cecum, taking into account different criteria such as high technical reproducibility, high bacterial diversity and easy handling. Read More

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http://dx.doi.org/10.1093/femsle/fnz033DOI Listing
March 2019
1 Read

Epidemiological linkage of vancomycin-resistant Enterococcus faecium from different sources in Ahwaz, Iran.

FEMS Microbiol Lett 2019 Mar 27. Epub 2019 Mar 27.

Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

This study was set to determine the genetic linkage and the clonal relationship between VRE faecium isolates in three hospitals of Ahwaz city. In this study, 1050 samples were collected from various rectal swabs, hands of health care workers (HCW), environmental surfaces, medical equipment, and 146 enterococci isolates from clinical sources of three hospitals from March to September 2015. Antimicrobial resistance patterns in VRE faecium were detected by disk diffusion method. Read More

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http://dx.doi.org/10.1093/femsle/fnz062DOI Listing
March 2019
1 Read

The seagrass holobiont: understanding seagrass-bacteria interactions and their role in seagrass ecosystem functioning.

FEMS Microbiol Lett 2019 Mar 18. Epub 2019 Mar 18.

Centre for Marine Ecosystems Research, Edith Cowan University, Joondalup, Western Australia, Australia.

This review shows that the presence of seagrass microbial community is critical for the development of seagrasses; from seed germination, through to phytohormone production and enhanced nutrient availability, and defence against pathogens and saprophytes. The tight seagrass-bacterial relationship highlighted in this review supports the existence of a seagrass holobiont and adds to the growing evidence for the importance of marine eukaryotic microorganisms in sustaining vital ecosystems. Incorporating a micro-scale view on seagrass ecosystems substantially expands our understanding of ecosystem functioning and may have significant implications for future seagrass management and mitigation against human disturbance. Read More

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http://dx.doi.org/10.1093/femsle/fnz057DOI Listing
March 2019
2 Reads

Effect of polyhexamethylene biguanide functionalized silver nanoparticles on the growth of Staphylococcus aureus.

FEMS Microbiol Lett 2019 Feb;366(4)

School of Life Sciences, Shanghai University, Shanghai 200444, PR China.

Polyhexamethylene biguanide (PHMB) was used as a stabilizing ligand to synthesize uniform silver nanoparticles (Ag NPs). The effects and action mechanism of PHMB functionalized Ag NPs (Ag NPs-PHMB) on the growth of the Gram-positive bacteria Staphylococcus aureus were investigated. The results showed that a high concentration Ag NPs-PHMB could be obtained and prepared particles were fairly uniform. Read More

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http://dx.doi.org/10.1093/femsle/fnz036DOI Listing
February 2019

Fermentation as a driver for food innovation.

FEMS Microbiol Lett 2019 Mar 16. Epub 2019 Mar 16.

Department of Food Science, Nordic Food Lab, Design and Consumer Behaviour, University of Copenhagen, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark.

The following paper is reviewing the current trends in research connected to fermentation, highlighting the work carried out in the various research kitchens that have gained a new understanding of fermentation in the recent years. The reflection on the developments made both on microbiological as well on the technological level aims to create pathways within the research fields in order to foster new innovations within the food production system. Read More

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http://dx.doi.org/10.1093/femsle/fnz058DOI Listing
March 2019
2 Reads

Molecular host mimicry and manipulation in bacterial symbionts.

Authors:
A Carolin Frank

FEMS Microbiol Lett 2019 Feb;366(4)

Life and Environmental Sciences, 5200 North Lake Rd, University of California Merced, Merced, CA 95343, USA.

It is common among intracellular bacterial pathogens to use eukaryotic-like proteins that mimic and manipulate host cellular processes to promote colonization and intracellular survival. Eukaryotic-like proteins are bacterial proteins with domains that are rare in bacteria, and known to function in the context of a eukaryotic cell. Such proteins can originate through horizontal gene transfer from eukaryotes or, in the case of simple repeat proteins, through convergent evolution. Read More

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http://dx.doi.org/10.1093/femsle/fnz038DOI Listing
February 2019

CRISPR-Cas, a highly effective tool for genome editing in Clostridium saccharoperbutylacetonicum N1-4(HMT).

FEMS Microbiol Lett 2019 Mar 15. Epub 2019 Mar 15.

Green Biologics Ltd, R&D labs, 154AH Brook Drive, Milton Park, Abingdon, UK. OX14 4SD.

The solventogenic clostridia have long been known for their ability to convert sugars from complex feedstocks into commercially important solvents. Although the acetone-butanol-ethanol (ABE) process fell out of favour decades ago, renewed interest in sustainability and 'green' chemistry has re-established our appetite for reviving technologies such as these, albeit with 21st century improvements. As CRISPR-Cas genome editing tools are being developed and applied to the solventogenic clostridia, their industrial potential is growing. Read More

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http://dx.doi.org/10.1093/femsle/fnz059DOI Listing
March 2019
1 Read

Evidence for the natural occurrence of Wolbachia in Aedes aegypti mosquitoes.

FEMS Microbiol Lett 2019 Mar 14. Epub 2019 Mar 14.

Molecular Microbiology Laboratory, Department of Biotechnology, Bharathiar University, Tamil Nadu, India.

Wolbachia, a gram-ve bacterium, is widely known to be present in arthropods and nematodes. Of late, great impetus is given to employ this intracellular bacterium, as an alternative to conventional biocontrol agents for the control of mosquitoes because of its inherent ability to induce sperm-egg incompatibility, feminization etc. By employing molecular tools, we have shown the presence of Wolbachia from Aedes aegypti mosquito population collected from Coimbatore, India by PCR amplifying the Ae. Read More

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http://dx.doi.org/10.1093/femsle/fnz055DOI Listing
March 2019
2 Reads

Sucrose-inducible heterologous expression of phytase in high cell density cultivation of the thermotolerant methylotrophic yeast Ogataea thermomethanolica.

FEMS Microbiol Lett 2019 Mar 14. Epub 2019 Mar 14.

Microbial Cell Factory Research Team, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand.

In this study, production of fungal phytase in thermotolerant methylotrophic yeast Ogataea thermomethanolica TBRC656 employing methanol inducible OtAOX promoter and sucrose inducible OtMal promoter was investigated in a high cell density fed-batch fermentation. Although a similar maximum cell concentration was obtained in both expression systems, the OtMal system gives approximately two-fold higher phytase activity, specific yield, production yield, volumetric productivity and specific productivity rate than the OtAOX system. In addition to being more efficient, the OtMal system is more flexible because sucrose or sugarcane molasses can be utilized as less expensive carbon sources instead of glycerol in batch and fed-batch stages. Read More

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https://academic.oup.com/femsle/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/femsle/fnz052DOI Listing
March 2019
12 Reads

Comparison of the expression of phospholipase D from Streptomyces halstedii in different hosts and its over-expression in Streptomyces lividans.

FEMS Microbiol Lett 2019 Mar 14. Epub 2019 Mar 14.

State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.

Phospholipase D (PLD) proteins from Streptomyces species are useful biocatalysts for synthesizing phospholipid derivatives relevant for the pharmaceutical and food industry from low-cost phosphatidylcholine. The overexpression of PLD in a recombinant strain is necessary to achieve large-scale PLD production. In this study, we investigated the feasibility of expressing PLD from Streptomyces halstedii in different hosts. Read More

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https://academic.oup.com/femsle/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/femsle/fnz051DOI Listing
March 2019
7 Reads

VqsA controls exotoxin production by directly binding to the promoter of asp in the pathogen Vibrio alginolyticus.

FEMS Microbiol Lett 2019 Mar 13. Epub 2019 Mar 13.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.

Quorum sensing (QS) system is an important bacterial cell-to-cell signaling system controlling expression of various genes in response to cell densities. In vibrios, LuxR/AphA are two established master QS regulators (MQSRs), and VqsA is recently identified to be the third putative MQSR. As a novel LysR-type regulator, the regulon and the underlying regulation mechanisms of VqsA remains to be elucidated. Read More

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http://dx.doi.org/10.1093/femsle/fnz056DOI Listing
March 2019
1 Read

A unique methylation pattern by a type I HsdM methyltransferase prepares for DpnI rare cutting sites in the Pseudomonas aeruginosa PAO1 genome.

FEMS Microbiol Lett 2019 Mar 13. Epub 2019 Mar 13.

Clinical research Group 'Pseudomonas Genomics', OE 6710, Hannover Medical School, 30625 Hannover, Germany.

A physical genome map of Pseudomonas aeruginosa PAO1 had been constructed in the late 1980 s by combinatorial SpeI/DpnI long-range restriction mapping. Whereas SpeI was known to cleave within the infrequent 5'-ACTAGT hexanucleotide, the rare recognition site 5'-Gm6ATC for the methylation-sensitive 4-cutter DpnI in the PAO1 genome remained elusive. Resequencing the PAO1 genome with the methylation-sensitive Single Molecule Real-Time (SMRT) Sequencing technology identified DpnI to cleave the PAO1 genome at 15 palindromic GAC(N)6Gm6ATC(N)6GTC recognition sites with the central Gm6ATC palindrome to be methylated on both strands by the clone PAO1-specific type I HsdM superfamily methyltransferase M. Read More

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http://dx.doi.org/10.1093/femsle/fnz053DOI Listing

A COLOR-BASED COMPETITION ASSAY FOR STUDYING BACTERIAL STRESS RESPONSES IN MICROCOCCUS LUTEUS.

FEMS Microbiol Lett 2019 Mar 13. Epub 2019 Mar 13.

Department of Biology and Biochemistry, The University of Houston, Houston, TX, USA.

Competition assays measure differences between populations of bacteria after stress adaptation, populations of different bacteria, and mutations in antibiotic resistance genes. We have developed a competition-based assay to evaluate if genes upregulated under starvation are important for bacterial survival. Stress responses are critical for survival in non-pathogenic and pathogenic bacteria alike including Mycobacterium tuberculosis, Enterococcus fecaelis, Escherichia coli, and Staphylococcus aureus. Read More

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https://academic.oup.com/femsle/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/femsle/fnz054DOI Listing
March 2019
13 Reads

Brazilian-adapted soybean Bradyrhizobium strains uncover IS elements with potential impact on biological nitrogen fixation.

FEMS Microbiol Lett 2019 Mar 12. Epub 2019 Mar 12.

GaTE Lab; Departamento de Botânica - Instituto de Biociências, Universidade de São Paulo, 277 Matão Street, 05508-090, São Paulo, SP, Brazil.

Bradyrhizobium diazoefficiens CPAC 7 and Bradyrhizobium japonicum CPAC 15 are broadly used in commercial inoculants in Brazil, contributing to most of the nitrogen required by the soybean crop. These strains differ in their symbiotic properties: CPAC 7 is more efficient in fixing nitrogen, whereas CPAC 15 is more competitive. Comparative genomics revealed many transposases close to genes associated with symbiosis in the symbiotic island of these strains. Read More

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http://dx.doi.org/10.1093/femsle/fnz046DOI Listing

Variations in early gut microbiome are associated with childhood eczema.

FEMS Microbiol Lett 2019 Mar 12. Epub 2019 Mar 12.

Coyote Diagnostics Lab (Beijing) Co., Ltd., Beijing, China.

We assessed the relationship between gut microbiome profile and childhood eczema in 172 subjects (age < 3 years, healthy group N = 123, eczema group N = 49) utilizing 16S rRNA gene sequencing. Lower relative abundance of Bifidobacterium was shown to be associated with childhood eczema. Considering that developmental and environmental factors could modify the state of children's gut microbiome, we divided the samples into four age groups: 0-0. Read More

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https://academic.oup.com/femsle/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/femsle/fnz020DOI Listing
March 2019
7 Reads

It's a small, small world: Unravelling the role and evolution of small RNAs in organelle and endosymbiont genomes.

FEMS Microbiol Lett 2019 Mar 7. Epub 2019 Mar 7.

Department of Entomology, University of California, Riverside, Riverside, CA, USA.

Organelles and host-restricted bacterial symbionts are characterized by having highly reduced genomes that lack many key regulatory genes and elements. Thus, it has been hypothesized that the eukaryotic nuclear genome is primarily responsible for regulating these symbioses. However, with the discovery of organelle and symbiont expressed small RNAs (sRNAs) there is emerging evidence that these sRNAs may play a role in gene regulation as well. Read More

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http://dx.doi.org/10.1093/femsle/fnz049DOI Listing
March 2019
1 Read

'Red - the magic color for solar salt production' - but since when?

FEMS Microbiol Lett 2019 Mar 6. Epub 2019 Mar 6.

State Key Laboratory of Palaeobiology and Stratigraphy, Nanjing Institute of Geology and Palaeontology and Center for Excellence in Life and Paleoenvironment, Chinese Academy of Sciences, 39, East Beijing Road, 210008 Nanjing, China.

Dense communities of carotenoid-rich members of the Halobacteria (Euryarchaeota), the bacterium Salinibacter (Bacteroidetes) and the eukaryotic alga Dunaliella color the brines of most saltern crystallizer ponds red. The first report we found from the western world mentioning these red brines dates from 1765: the Encyclopédie of Diderot and coworkers. Earlier descriptions of solar salterns since Roman times do not mention red ponds. Read More

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http://dx.doi.org/10.1093/femsle/fnz050DOI Listing
March 2019
1 Read

A comparison of FIL-IDF/EFFCA Inventory of microbial food cultures and Efsa biohazard qualified presumption of safety.

FEMS Microbiol Lett 2019 Mar 6. Epub 2019 Mar 6.

EFFCA - European Food & Feed Cultures Association, Avenue de Tervueren 188A, Postbox 4 • 1150, Brussels, BELGIUM & DuPont Nutrition Biosciences ApS, Langebrogade 1, DK-1411 Copenhagen K, Denmark.

In order to provide a harmonised pre-assessment to support risk assessment performed by the European Food Safety Authority, the Biohazard Panel in 2007 published the guidelines for evaluation of safety of a strain included in the food chain, the Qualified Presumption of Safety (QPS). Since 2008, the Biohazard Panel publishes on a regular basis an update of the microbial strains submitted for approval and extends the list of species that have been granted a QPS status. The International Dairy Federation (IDF) and the European Food and Feed Cultures Association (EFFCA) has since 2002 been conducting a project on the safety demonstration of microbial food cultures (MFC). Read More

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http://dx.doi.org/10.1093/femsle/fnz048DOI Listing
March 2019
2 Reads

Addiction systems antagonize bacterial adaptive immunity.

FEMS Microbiol Lett 2019 Mar 5. Epub 2019 Mar 5.

Environment and Sustainability Institute, University of Exeter, Penryn TR10 9FE, UK.

CRISPR-Cas systems provide adaptive immunity against mobile genetic elements, but employment of this resistance mechanism is often reported with a fitness cost for the host. Whether or not CRISPR-Cas systems are important barriers for the horizontal spread of conjugative plasmids, which play a crucial role in the spread of antibiotic resistance, will depend on the fitness costs of employing CRISPR-based defences and the benefits of resisting conjugative plasmids. To estimate these costs and benefits we measured bacterial fitness associated with plasmid immunity using Escherichia coli and the conjugative plasmid pOX38-Cm. Read More

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http://dx.doi.org/10.1093/femsle/fnz047DOI Listing
March 2019
4 Reads

Transition into a New Job.

Authors:

FEMS Microbiol Lett 2019 Feb 26. Epub 2019 Feb 26.

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http://dx.doi.org/10.1093/femsle/fnz045DOI Listing
February 2019
1 Read

Vertical stratification of bacteria and archaea in sediments of a small boreal humic lake.

FEMS Microbiol Lett 2019 Feb 26. Epub 2019 Feb 26.

University of Eastern Finland, Department of Environmental and Biological Sciences, Kuopio, Finland.

Although sediments of small boreal humic lakes are important carbon stores and greenhouse gas sources, the composition and structuring mechanisms of their microbial communities have remained understudied. We analyzed the vertical profiles of microbial biomass indicators (PLFAs, DNA and RNA) and the bacterial and archaeal community composition (sequencing of 16S rRNA gene amplicons and qPCR of mcrA) in sediment cores collected from a typical small boreal lake. While microbial biomass decreased with sediment depth, viable microbes (RNA and PLFA) were present all through the profiles. Read More

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http://dx.doi.org/10.1093/femsle/fnz044DOI Listing
February 2019
1 Read

Loop-mediated isothermal amplification assay for Enterococcus sp., E. coli and S. aureus in chicken.

FEMS Microbiol Lett 2019 Feb 26. Epub 2019 Feb 26.

Avian disease division, Animal and Plant Quarantine Agency, HyukSin 8-ro, GimCheon, Republic of Korea.

Bacterial chondronecrosis with osteomyelitis (BCO) is a major cause of lameness in broiler chicken, and results in serious economic losses worldwide. Although the pathogenesis mechanism leading to lameness is not entirely understood, some strains of Enterococcus sp., avian pathogenic Escherichia coli, or Staphylococcus aureus have been long recognized as important causative pathogens. Read More

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http://dx.doi.org/10.1093/femsle/fnz042DOI Listing
February 2019
1 Read

Modification of T2 phage infectivity toward Escherichia coli O157:H7 via using CRISPR/Cas9.

FEMS Microbiol Lett 2019 Feb;366(4)

School of Life Science and Technology, Tokyo Institute of Technology, 4259 J2-15 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.

Phage therapy is getting considerable attention as a method for prophylaxis of food poisoning caused by Escherichia coli O157:H7, an important pathogen causing life-threatening bloody diarrhea. Despite previous studies have shown the feasibility of phage therapy to E. coli O157:H7, promising results have not been obtained in vivo yet. Read More

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http://dx.doi.org/10.1093/femsle/fnz041DOI Listing
February 2019
2 Reads

'Candidatus Viridilinea mediisalina', a novel phototrophic Chloroflexi bacterium from a Siberian soda lake.

FEMS Microbiol Lett 2019 Feb 25. Epub 2019 Feb 25.

Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky ave. 33, bld. 2, Moscow 119071, Russian Federation.

In this article, we present the description of a novel mesophilic phototrophic Chloroflexi bacterium, 'Candidatus Viridilinea mediisalina' Kir15-3F. We have isolated an anaerobic, highly-enriched culture of this bacterium from the Kiran soda lake (Siberia) and optimized its cultivation. Metagenomic sequencing revealed that 'Ca. Read More

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http://dx.doi.org/10.1093/femsle/fnz043DOI Listing
February 2019
1 Read

I'm the one that you want….

Authors:
Beatrix Fahnert

FEMS Microbiol Lett 2019 Feb;366(3)

School of Life Sciences, University of Lincoln, Green Lane, Lincoln LN6 7TS, UK.

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http://dx.doi.org/10.1093/femsle/fnz028DOI Listing
February 2019
2 Reads

Immunogenicity characterization of genetically fused or chemically conjugated heat-stable toxin toxoids of enterotoxigenic Escherichia coli in mice and pigs.

FEMS Microbiol Lett 2019 Feb;366(4)

Department of Diagnostic Medicine/Pathobiology, Kansas State University College of Veterinary Medicine, Manhattan, Kansas, USA.

Enterotoxigenic Escherichia coli (ETEC) producing type Ib heat-stable toxin (STa) are a main cause of children's diarrhea and travelers' diarrhea, thus STa needs to be targeted in ETEC vaccine development. However, because this 19-amino acid STa is poorly immunogenic, attempts to genetically fuse or chemically couple it to carrier proteins have been made to enhance STa immunogenicity. In this study, we selected one genetic fusion and one chemical conjugate to comparatively evaluate STa immunogenicity. Read More

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http://dx.doi.org/10.1093/femsle/fnz037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420877PMC
February 2019
4 Reads

Seasonal changes in luminescent intestinal microflora of the fish inhabiting the Bering and Okhotsk seas.

FEMS Microbiol Lett 2019 Feb;366(4)

Laboratory of Molecular Genetics, Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region 141701, Russian Federation.

Here, we present a study of luminescent intestinal microflora of the fish inhabiting Bering and Okhotsk seas in summer and winter seasons. Sampling of intestinal luminescent microflora was carried for several years, with all recovered species belonging to psychrophilic bacteria of either Aliivibrio logei or Photobacterium phosphoreum species. A seasonal change in fish intestinal luminescent microflora detected include an increase in prevalence of P. Read More

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http://dx.doi.org/10.1093/femsle/fnz040DOI Listing
February 2019
6 Reads

Probiotic Enterococcus faecalis AG5 effectively assimilates cholesterol and produces fatty acids including propionate.

FEMS Microbiol Lett 2019 Feb;366(4)

Department of Integrative Biology, School of BioSciences and Technology, VIT, Vellore-632014, Tamil Nadu, India.

Probiotics are known for their health-promoting abilities through the production of various metabolites, and also benefits are membrane associated. Probiotic Enterococcus faecalis AG5 has been previously assessed for its probiotic properties and this study further strengthens that claim. Strain AG5 was inoculated with 0. Read More

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http://dx.doi.org/10.1093/femsle/fnz039DOI Listing
February 2019
1 Read
2.121 Impact Factor

The potential of pectin to impact pig nutrition and health: feeding the animal and its microbiome.

Authors:
Maria Wiese

FEMS Microbiol Lett 2019 Feb;366(4)

Department of Food Science, University of Copenhagen, Rolighedsvej 26, 1958 Frederiksberg, Denmark.

The increasing efforts to substitute antibiotics and improve animal health combined with the acknowledgement of the role of gut microbiota in health have led to an elevated interest in the understanding on how fibre with prebiotic potential, such as pectin, can improve animal growth and health via direct or gut microbiota mediated effects. Various reports exist on the antiviral and antibacterial effects of pectin, as well as its potency as a modulator of the immune response and gut microbial community. Comprehensive insights into the potential of pectin to improve animal growth and health are currently still hampered by heterogeneity in the design of studies. Read More

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http://dx.doi.org/10.1093/femsle/fnz029DOI Listing
February 2019
3 Reads

Guillain-Barre Syndrome and Zika infection: Identifying leading producers, countries relative specialization, and collaboration.

FEMS Microbiol Lett 2019 Feb 13. Epub 2019 Feb 13.

Dept. Nursing and Physiotherapy, University of Cádiz, Cádiz (Spain).

Zika virus (Zikv) infection implies a significant public health issue due to the different syndromes associated. It is related to several central nervous system problems, such as microcephaly and Guillain-Barre Syndrome. In this way, the first document about the relation of Guillain-Barre Syndrome and Zika indexed in Web of Science was published in 2014. Read More

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http://dx.doi.org/10.1093/femsle/fnz035DOI Listing
February 2019
1 Read

Resveratrol controls Escherichia coli growth by inhibiting the AcrAB-TolC efflux pump.

FEMS Microbiol Lett 2019 Feb;366(4)

Department of Public Health Science (BK21 PLUS Program), Graduate School, Korea University, Seoul, South Korea.

Resveratrol (RSV), a phytoalexin found in grapes and other plants, is known to have antibacterial effects against Escherichia coli. In this study, we aimed to identify the target gene(s) for the antibacterial activity of RSV in E. coli. Read More

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http://dx.doi.org/10.1093/femsle/fnz030DOI Listing
February 2019
3 Reads

An IS26 variant with enhanced activity.

FEMS Microbiol Lett 2019 Feb;366(3)

School of Life and Environmental Sciences, Molecular Bioscience Building G08, Cnr Maze Crescent and Butlin Avenue, The University of Sydney, NSW 2006, Australia.

The insertion sequence IS26 plays a major role in the mobilization, expression and dissemination of antibiotic resistance genes in Gram-negative bacteria. Though IS26 is abundant in sequenced genomes and in plasmids that harbour antibiotic resistance genes, only a few minor variations in the IS26 sequence have been recorded. The most common variant, IS26* (also known as IS15Δ1), encodes a Tnp26 transposase with a single amino acid substitution, G184N in the catalytic domain. Read More

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http://dx.doi.org/10.1093/femsle/fnz031DOI Listing
February 2019

Purification and characteristics of an aflatoxin B1 degradation enzyme isolated from Pseudomonas aeruginosa.

FEMS Microbiol Lett 2019 Feb 11. Epub 2019 Feb 11.

School of Food Science and Technology, Henan University of Technology, Zhengzhou, Henan 450001, People's Republic of China.

Aflatoxin is a highly toxic mycotoxin produced by Aspergillus flavus and A.parasitic fungus. Since it contaminates food and grain widely, it has seriously endanger the health of human beings and animals. Read More

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http://dx.doi.org/10.1093/femsle/fnz034DOI Listing
February 2019
6 Reads
2.121 Impact Factor