22,051 results match your criteria FEMS microbiology letters[Journal]


New CRISPR-Cas9 vectors for genetic modifications of Bacillus species.

FEMS Microbiol Lett 2018 Dec 6. Epub 2018 Dec 6.

Institut für Industrielle Genetik, Universität Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.

Genetic manipulation is a fundamental procedure for the study of gene and operon functions and new characteristics acquisition. Modern CRISPR-Cas technology allows genome editing more precise and increases the efficiency of transferring mutations in a variety of hard to manipulate organisms. Here, we describe new CRISPR-Cas vectors for genetic modifications in bacillary species. Read More

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December 2018
1 Read

Erratum to: 'Dysbiosis and early mortality in zebrafish larvae exposed to subclinical concentrations of streptomycin'.

FEMS Microbiol Lett 2018 Dec;365(24)

Department of Biology, Reem-Kayden Center for Science and Computation, Bard College, Annandale-On-Hudson, NY, 12504, USA.

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December 2018

Diversity and metabolism of xylose and glucose fermenting microbial communities in sequencing batch or continuous culturing.

FEMS Microbiol Ecol 2018 Dec 3. Epub 2018 Dec 3.

Delft University of technology, Department of Biotechnology, Van der Maasweg 9, 2629 HZ Delft, the Netherlands.

A mechanistic understanding of microbial community establishment and product formation in open fermentative systems can aid the development of bioprocesses utilising organic waste. Kinetically, a single rate-limiting substrate is expected to result in one dominant species. Four enrichment cultures were operated to ferment either xylose or glucose in a sequencing batch reactor (SBR) or a continuous-flow stirred tank reactor (CSTR) mode. Read More

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December 2018

Secretion and signalling of bacterial RNAs.

FEMS Microbiol Lett 2018 Dec 4. Epub 2018 Dec 4.

The Life Science Centre - Biology, School of Science and Technology, Örebro University, 701 82 Örebro, Sweden.

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December 2018

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as a useful tool for the rapid identification of wild flea vectors preserved in alcohol.

Med Vet Entomol 2018 Dec 5. Epub 2018 Dec 5.

Institut de Recherche pour le Développement (IRD), Assistance Publique-Hôpitaux de Marseille (AP-HM), Service de Santé des Armées (SSA), Vecteurs-Infections Tropicales et Méditerranéennes (VITROME), Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Aix Marseille University, Marseille, France.

Flea identification is a significant issue because some species are considered as important vectors of several human pathogens that have emerged or re-emerged recently, such as Bartonella henselae (Rhizobiales: Bartonellaceae) and Rickettsia felis (Rickettsiales: Rickettsiaceae). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been evaluated in recent years for the identification of multicellular organisms, including arthropods. A preliminary study corroborated the usefulness of this technique for the rapid identification of fleas, creating a preliminary database containing the spectra of five species of flea. Read More

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December 2018
1 Read

Nitrogen-dependent induction of atrazine degradation pathway in Pseudomonas sp. strain AKN5.

FEMS Microbiol Lett 2018 Nov 30. Epub 2018 Nov 30.

Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400 076, India.

Soil isolate Pseudomonas sp. strain AKN5 degrades atrazine as the sole source of nitrogen. The strain showed expeditious growth on medium containing citrate as the carbon source and ammonium chloride as the nitrogen source as compared to citrate plus atrazine or cyanuric acid. Read More

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November 2018

Effect of cytochrome bc1 complex inhibition during fermentation and growth of Scheffersomyces stipitis using glucose, xylose, or arabinose as carbon sources.

FEMS Yeast Res 2018 Nov 30. Epub 2018 Nov 30.

DIPA, PROPAC. Facultad de Química, Universidad Autónoma de Querétaro, C.U. Cerro de las Campanas s/n. Col. Las Campanas, C.P. 76010 Querétaro, Qro., México.

Scheffersomyces stipitis shows a high capacity to ferment xylose, with a strong oxygen dependence to allow NAD+ regeneration. However, without oxygen regeneration of NADH occurs by other metabolic pathways like alcoholic fermentation. There are few reports about inhibitors of mitochondrial respiration and their effects on growth and fermentation. Read More

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November 2018

Calcium ion induces outer membrane fusion of Deinococcus grandis spheroplasts to generate giant spheroplasts with multiple cytoplasms.

FEMS Microbiol Lett 2018 Nov 30. Epub 2018 Nov 30.

Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan.

Generally, enlarged spheroplasts of the Gram-negative bacterium Deinococcus grandis contain a single cytoplasm and a large periplasmic space. Enlargement of D. grandis spheroplasts requires the presence of divalent cation Ca2+ or Mg2+. Read More

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November 2018

Highly competitive fungi manipulate bacterial communities in decomposing beech wood (Fagus sylvativa).

FEMS Microbiol Ecol 2018 Nov 29. Epub 2018 Nov 29.

Cardiff School of Biosciences, Cardiff University, CF10 3AX, Wales, UK.

The bacterial communities in decomposing wood are receiving increased attention, but their interactions with wood-decay fungi are poorly understood. This is the first field study to test the hypothesis that fungi are responsible for driving bacterial communities in beech wood (Fagus sylvatica). A meta-genetic approach was used to characterise bacterial and fungal communities in wood that had been laboratory-colonised with known wood-decay fungi, and left for a year at six woodland sites. Read More

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November 2018
2 Reads

The Suhomyces clade: from single isolate to multiple species to disintegrating sex loci.

FEMS Yeast Res 2018 Nov 28. Epub 2018 Nov 28.

Louisiana State University, Biological Sciences; University of South Carolina, Biological Sciences.

Candida tanzawaensis clade members are now placed in Suhomyces. The group was virtually unknown until the early 2000 s. Here we review progress made on Suhomyces over the last two decades and provide data from reports of new members of the group from distant localities worldwide, their habitats and a new study of mating loci that helps explain earlier failed compatibility tests. Read More

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November 2018

Spruce and beech as local determinants of forest fungal community structure in litter, humus and mineral soil.

FEMS Microbiol Ecol 2018 Nov 27. Epub 2018 Nov 27.

Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences, P.O. Box 5003, NO-1432 Ås, Norway.

Beech forests reaches its native distribution limit in SE Norway, but is expected to expand substantially northwards due to climate warming. This may potentially result in a fundamental transformation of contemporary Northern European forests, with tentative effects on the associated belowground fungi. Fungal communities mediate vital ecosystem processes such as ecosystem productivity and carbon sequestration in boreal forests. Read More

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November 2018
7 Reads

Deforestation Impacts Network Co-occurrence Patterns of Microbial Communities in Amazon Soils.

FEMS Microbiol Ecol 2018 Nov 27. Epub 2018 Nov 27.

Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.

Co-occurrence networks allow for the identification of potential associations among species, which may be important for understanding community assembly and ecosystem functions. We employed this strategy to examine prokaryotic co-occurrence patterns in the Amazon soils and the response of these patterns to land use change to pasture, with the hypothesis that altered microbial composition due to deforestation will mirror the co-occurrence patterns across prokaryotic taxa. In this study, we calculated Spearman correlations between operational taxonomic units (OTUs) as determined by 16S rRNA gene sequencing, and only robust correlations were considered for network construction (-0. Read More

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November 2018

A newly isolated Enterobacter sp. strain produces 2,3-butanediol during its cultivation on low-cost carbohydrate-based substrates.

FEMS Microbiol Lett 2018 Nov 26. Epub 2018 Nov 26.

Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, Athens 11855, Greece.

2,3-Butanediol (BDO) is an important platform chemical with wide range of applications in various industries. In the present study, a newly isolated wild Enterobacter sp. strain (FMCC-208) was evaluated towards its ability to produce BDO on media composed of sugars derived from sucrose refinery plant. Read More

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November 2018

Nitrogen availability facilitates phosphorus acquisition by bloom-forming cyanobacteria.

Authors:
Luis Aubriot

FEMS Microbiol Ecol 2018 Nov 21. Epub 2018 Nov 21.

Grupo de Ecología y Fisiología de Fitoplancton, Sección Limnología, Facultad de Ciencias, Universidad de la República, Iguá 4255, CP11400, Montevideo, Uruguay.

Cyanobacterial blooms are threatening freshwater ecosystems. The physiological basis involved in the onset of cyanobacterial bloom is fundamental to advance in bloom predictions. Generally, cyanobacteria grow until the availability of nitrogen (N), phosphorus (P), or both nutrients becomes limited. Read More

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November 2018

Evaluation of human trophoblasts and ovine testis cell lines for the study of the intracellular pathogen Brucella ovis.

FEMS Microbiol Lett 2018 Dec;365(24)

Departamento de Microbiología y Genética, Edificio Departamental, Universidad de Salamanca, Plaza Doctores de la Reina s/n, 37007 Salamanca, Spain.

Since pathogenic Brucella survive and replicate inside phagocytes, cellular models of infection constitute important tools in brucellosis research. We describe the behavior of B. ovis PA (which causes a type of ovine brucellosis mainly affecting the male reproductive tract) and representative attenuated mutants in two commercially available cell lines of non-professional phagocytes related to Brucella tissue preference: OA3. Read More

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December 2018

Toxin-antitoxin systems shows variability among Mycobacterium tuberculosis lineages.

FEMS Microbiol Lett 2018 Nov 26. Epub 2018 Nov 26.

Corporación para investigaciones Biológicas (CIB).

The Toxin-Antitoxin (TA) systems are operons involved in the formation of persistent cells and in stress situations in microorganism. TAs are widely distributed in Mycobacterium tuberculosis (MTB). The objective of this study was to determine the distribution and variability of protein sequences of TA systems in seven MTB lineages. Read More

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November 2018

Planctomycetes in boreal and subarctic wetlands: diversity patterns and potential ecological functions.

FEMS Microbiol Ecol 2019 Feb;95(2)

Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, Leninsky prospect 33-2, Russia.

Members of the phylum Planctomycetes are common inhabitants of boreal Sphagnum peat bogs and lichen-dominated tundra wetlands. These bacteria colonize both oxic and anoxic peat layers and reach the population size of 107 cells per gram of wet peat. The 16S rRNA gene sequences from planctomycetes comprise 5%-22% of total 16S rRNA gene reads retrieved from peat samples. Read More

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February 2019

A system for the expression and release of heterologous proteins from the core of Bacillus subtilis spores.

FEMS Microbiol Lett 2018 Dec;365(23)

Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge CB3 0AS, UK.

Methodologies that exploit the durability of Bacillus subtilis spores by displaying heterologous proteins or antigenic molecules on the spore surface for mucosal vaccine delivery and other applications are well established. Here we extend the concept by engineering spores intended as oral delivery vehicles for therapeutic proteins. The method is exemplified by the expression and deposition of human growth hormone in the developing spore core, where the protein is shielded from physicochemical and biological degradation by the protective spore structure. Read More

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December 2018

Extracellular membrane vesicles (EVs) in the three domains of life and beyond.

FEMS Microbiol Rev 2018 Nov 21. Epub 2018 Nov 21.

Institute for Integrative Biology of the Cell (I2BC), Biologie Cellulaire des Archées (BCA), CEA, CNRS, Université Paris-Sud, 91405 Orsay cedex, France.

Cells from all three domains of life, Archaea, Bacteria and Eukarya, produce extracellular vesicles (EVs) which are sometimes associated to filamentous structures known as nanopods or nanotubes. The mechanisms of EV biogenesis in the three domains remain poorly understood, although studies in Bacteria and Eukarya indicate that the regulation of lipid composition plays a major role in initiating membrane curvature. EVs are increasingly recognized as important mediators of intercellular communication via transfer of a wide variety of molecular cargoes. Read More

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November 2018
6 Reads

Nicotine enhances the thickness of biofilm and adherence of Candida albicans ATCC 14053 and Candida parapsilosis ATCC 22019.

FEMS Yeast Res 2018 Nov 21. Epub 2018 Nov 21.

Department of Oral and Craniofacial Sciences, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.

Candida albicans ATCC 14053 and Candida parapsilosis ATCC 22019 HWP1 (hyphal-wall protein 1) are involved in hyphae formation and pathogenesis. The transcriptional ALS3 (agglutinin-like sequence 3) gene in both species are responsible for the development of biofilm and colonization on tooth surfaces. Therefore, we investigated the expression of HWP1 and ALS3 quantitatively in C. Read More

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November 2018
5 Reads

Molecular cloning and characterization of a methyl parathion hydrolase from an organophosphorus-degrading bacterium, Serratia marcescens MEW06.

FEMS Microbiol Lett 2018 Dec;365(24)

State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, School of Life Sciences, Hubei University, Wuhan, 430062, PR China.

An organophosphorus-degrading bacterium MEW06, which exhibited excellent biodegradation capabilities towards 50 mg/L of methyl parathion (MP), paraoxon and dimethoate, was isolated from Sand Lake (Wuhan, China) and identified as Serratia marcescens subsp. marcescens based on physiological-biochemical characteristics and a 16S rDNA sequence-based phylogenetic tree. MEW06 genome contains a 31. Read More

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December 2018

Virulence factor-dependent basolateral invasion of choroid plexus epithelial cells by pathogenic Escherichia coli in vitro.

FEMS Microbiol Lett 2018 Nov 21. Epub 2018 Nov 21.

Pediatric Infectious Diseases, Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, Theodor-Kutzer-Ufer 1-3, D-68167 Mannheim, Germany.

Escherichia coli (E. coli) is the most common Gram-negative causative agent of neonatal meningitis and E. coli meningitis is associated with a high morbidity and mortality. Read More

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November 2018
2 Reads

Genomic insights into the metabolism of 'Candidatus Defluviicoccus seviourii', a member of Defluviicoccus cluster III abundant in industrial activated sludge.

FEMS Microbiol Ecol 2018 Nov 26. Epub 2018 Nov 26.

Department of Wine & Food Science, University of Adelaide.

Filamentous cluster III Defluviicoccus (DF3) are known to proliferate and cause bulking issues in industrial wastewater treatment plants. Members of the genus Defluviicoccus are also known to exhibit the glycogen accumulating organism (GAO) phenotype, which is suggested to be detrimental to enhanced biological phosphorus removal (EBPR). Despite the reported negative impact members of the DF3 have on activated sludge wastewater treatment systems, limited research has focused on understanding the physiological traits that allow them to compete in these environments. Read More

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November 2018
2 Reads

Deletion of GLX3 in Candida albicans affects temperature tolerance, biofilm formation, and virulence.

FEMS Yeast Res 2018 Nov 21. Epub 2018 Nov 21.

Departamento de Microbiología y Ecología. Universidad de Valencia. 46100-E. Burjassot. Spain.

Candida albicans is a predominant cause of fungal infections in mucosal tissues as well as life-threatening bloodstream infections in immunocompromised patients. Within the human body, C. albicans is mostly embedded in biofilms, which provides increased resistance to antifungal drugs. Read More

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November 2018

The morphology and metabolic potential of the Chloroflexi in full-scale activated sludge wastewater treatment plants.

FEMS Microbiol Ecol 2018 Nov 22. Epub 2018 Nov 22.

Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark.

Filamentous bacteria belonging to the phylum Chloroflexi have received considerable attention in wastewater treatment systems for their suggested role in the operational problem of impaired sludge settleability known as bulking. Their consistently high abundance in full-scale systems, even in the absence of bulking, indicates that they make a substantial contribution to the nutrient transformations during wastewater treatment. In this study, extensive 16S rRNA amplicon surveys of Danish wastewater treatment plants (WWTPs) with nutrient removal were screened to identify numerically important Chloroflexi genera. Read More

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November 2018
10 Reads

Mechanical Effects of Cellulose, Xyloglucan, and Pectins on Stomatal Guard Cells of .

Front Plant Sci 2018 5;9:1566. Epub 2018 Nov 5.

Department of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, PA, United States.

Stomata function as osmotically tunable pores that facilitate gas exchange at the surface of plants. Stomatal opening and closure are regulated by turgor changes in guard cells that result in mechanically regulated deformations of guard cell walls. However, how the molecular, architectural, and mechanical heterogeneities that exist in guard cell walls affect stomatal dynamics is unclear. Read More

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November 2018
3 Reads

Genetic engineering of Ehrlich pathway modulate production of higher alcohols in engineered Yarrowia lipolytica.

FEMS Yeast Res 2018 Nov 18. Epub 2018 Nov 18.

Micalis Institute, INRA-AgroParisTech, UMR1319, Team BIMLip: Integrative Metabolism of Microbial Lipids, Domaine de Vilvert, 78352 Jouy-en-Josas, France.

Microbial cells can produce a vast spectrum of chemical compounds, including those most desired by the global chemical market, as for example higher alcohols, which are promising alternative fuels and chemical feedstock. In the current research, we investigated the effects of the Ehrlich pathway genetic engineering on higher alcohols production in Y. lipolytica, which directly follows our previous findings concerning elucidation of putative molecular identities involved in this pathway. Read More

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November 2018
4 Reads

Klebsiella pneumoniae infection biology: living to counteract host defences.

FEMS Microbiol Rev 2018 Nov 18. Epub 2018 Nov 18.

Wellcome-Wolfson Institute for Experimental Medicine, Queen's University Belfast, Belfast, United Kingdom.

Klebsiella species cause a wide range of diseases including pneumonia, UTIs, bloodstream infections, and sepsis. These infections are particularly a problem among neonates, elderly and immunocompromised individuals. Klebsiella is also responsible for a significant number of community-acquired infections. Read More

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November 2018
10 Reads

Metabarcoding successfully tracks temporal changes in eukaryotic communities in coastal sediments.

FEMS Microbiol Ecol 2018 Nov 18. Epub 2018 Nov 18.

University of Helsinki, Faculty of Biological and Environmental Sciences, Ecosystems and Environment Research Program, P.O. Box 65 (Viikinkaari 1), FI-00014 University of Helsinki, Finland.

Metabarcoding is a method that combines high-throughput DNA sequencing and DNA based identification. Previously, this method has been successfully used to target spatial variation of eukaryote communities in marine sediments, however, the temporal changes in these communities remain understudied. Here, we follow the temporal changes of the eukaryote communities in Baltic Sea surface sediments collected from two coastal localities during three seasons of two consecutive years. Read More

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November 2018

The soil microbial community of turf: linear and nonlinear changes of taxa and N-cycle gene abundances over a century long turf development.

FEMS Microbiol Ecol 2018 Nov 15. Epub 2018 Nov 15.

Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC 27695, USA.

Turf, consisting of closely-spaced grasses and the subtending soil, is a unique ecosystem subject to intense management. Yet, soil organic matter accumulates quickly and reaches equilibrium after 20 to 50 years. Resource availability is an important driver of species richness and theoretically their relationship is expected to be unimodal. Read More

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November 2018
2 Reads

Distinct bacterial and archaeal diversities and spatial distributions in surface sediments of the Arctic Ocean.

FEMS Microbiol Lett 2018 Dec;365(24)

School of Minerals Processing and Bioengineering, Central South University, 410083 Changsha, P.R. China.

Environmental changes in the polar oceans are more pronounced than in other regions. The lack of knowledge regarding the microbial diversity, distribution and succession in the polar oceans has hampered our understanding about the effects of environmental changes on microbial ecosystems. This study investigated bacterial and archaeal diversities and spatial distributions in surface sediments of the Bering Sea and the Chukchi Sea. Read More

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December 2018
6 Reads

Fermenters in the Earthworm Gut: Do Transients Matter?

FEMS Microbiol Ecol 2018 Nov 16. Epub 2018 Nov 16.

Department of Ecological Microbiology, University of Bayreuth, 95440 Bayreuth, Germany.

Earthworms have profound impact on soil-based ecosystems. Although theoretical considerations suggest that most microbes in the earthworm gut are likely ingested and transient, the non-responsiveness of soil microbes to a specific high value gut nutrient and anoxia has made it difficult to demonstrate that responsive gut fermenters are derived from soil. Therefore, soil and gut content of the model earthworm Lumbricus terrestris were examined for their fermentative capabilities. Read More

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November 2018
3 Reads

Wood decomposition in aquatic and terrestrial ecosystems in the tropics: contrasting biotic and abiotic processes.

FEMS Microbiol Ecol 2019 Jan;95(1)

Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Wood decomposition, a critical process in carbon and nutrient cycles, is influenced by environmental conditions, decomposer communities and substrate composition. While these factors differ between land and stream habitats, across-habitat comparisons of wood decay processes are rare, limiting our ability to evaluate the context- dependency of the drivers of decay. Here we tracked wood decomposition of three tree species placed in stream and terrestrial habitats in a lowland tropical forest in Panama. Read More

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January 2019

Diversity of endophytic fungi of Paeonia lactiflora Pallas and screening for fungal paeoniflorin producers.

FEMS Microbiol Lett 2018 Dec;365(24)

Department of Biological and Food Engineering, Bozhou University, Bozhou, Anhui 236800, China.

Endophytic fungi from Paeonia lactiflora Pallas, which is mainly distributed in China, were characterized and screened to identify those capable of producing paeoniflorin. A total of 101 isolates obtained from the roots, stems and leaves of P. lactiflora were grouped into 16 fungal taxa based on morphological traits and internal transcribed spacers sequences, indicating that endophytic fungi of P. Read More

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December 2018
4 Reads

Exposure to the lampricide TFM elicits an environmental stress response in yeast.

FEMS Yeast Res 2019 Jan;19(1)

Department of Mathematics, Norwich University, 158 Harmon Drive, Northfield, VT 05663 USA.

The pesticide 3-trifluoromethyl-4-nitrophenol (TFM) is used to control sea lamprey populations in the Great Lakes and Lake Champlain. Little is known about the effects of this pesticide on gene expression in eukaryotic species. This study used microarray analysis to determine the effects of short term, high dose TFM exposure on gene expression in Saccharomyces cerevisiae. Read More

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January 2019
3 Reads

Proteins and RNA sequences required for the transition of the t-Utp complex into the SSU processome.

FEMS Yeast Res 2019 Jan;19(1)

West Virginia University Department of Biology Morgantown, West Virginia 26506.

Ribosomes are synthesized by large ribonucleoprotein complexes cleaving and properly assembling highly structured rRNAs with ribosomal proteins. Transcription and processing of pre-rRNAs are linked by the transcription-Utp sub-complex (t-Utps), a sub-complex of the small subunit (SSU) processome and prompted the investigations for the requirements of t-Utp formation and transition into the SSU processome. The rDNA promoter, the first 44 nucleotides of the 5΄ETS, and active transcription by pol I were sufficient to recruit the t-Utps to the rDNA. Read More

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January 2019

The Enhancement of Pyridine Degradation by Rhodococcus KDPy1 in Coking Wastewater.

FEMS Microbiol Lett 2018 Nov 15. Epub 2018 Nov 15.

College of Life Science, University of Chinese Academy of Sciences, A19 Yuquan Road, Beijing 100049, China.

Pyridine is a typical nitrogen heterocyclic and recalcitrant organic compound in coking wastewater. The pyridine-degrading bacterial strain KDPy1 was isolated from aerobic sludge in a coking wastewater treatment plant. The homology analysis based on 16S rDNA sequences suggested that KDPy1 belonged to Rhodococcus sp. Read More

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November 2018

The molecular biology of fruity and floral aromas in beer and other alcoholic beverages.

FEMS Microbiol Rev 2018 Nov 15. Epub 2018 Nov 15.

Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KU Leuven.

Aroma compounds provide attractiveness and variety to alcoholic beverages. We discuss the molecular biology of a major subset of beer aroma volatiles, fruity and floral compounds, originating from raw materials (malt and hops), or formed by yeast during fermentation. We introduce aroma perception, describe the most aroma-active, fruity and floral compounds in fruits and their presence and origin in beer. Read More

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November 2018
13 Reads

Description of two new species of Aliinostoc and one new species of Desmonostoc from India based on the Polyphasic Approach and reclassification of Nostoc punensis to Desmonostoc punense comb. nov.

FEMS Microbiol Lett 2018 Dec;365(24)

National Centre for Microbial Resource (NCMR), National Centre for Cell Science (NCCS), Pune-411021, Maharashtra, India.

Three heterocytous cyanobacterial strains were isolated from different habitats of Central India, and initial morphological studies indicated them to be members of the genus Nostoc or other closely related genera. Subsequent studies using morphological, ecological, molecular and phylogenetic methods indicated the three strains to be new members of the genera Aliinostoc and Desmonostoc. Folding of the D1-D1' helix of the ITS region clearly differentiated the three strains from the other closely related strains, thus providing final indications of the strains being different and new additions to the genera Aliinostoc and Desmonostoc. Read More

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December 2018
2 Reads

An Acetobacterium strain isolated with metallic iron as electron donor enhances iron corrosion by a similar mechanism as Sporomusa sphaeroides.

FEMS Microbiol Ecol 2018 Nov 15. Epub 2018 Nov 15.

Center for Microbial Ecology and Technology (CMET), Ghent University, Coupure Links 653, 9000 Ghent, BELGIUM.

Sporomusa sphaeroides related strains are to date the only homoacetogens known to increase metallic iron corrosion. The goal of this work was to isolate additional homoacetogenic bacteria capable of using Fe(0) as electron donor and to explore their extracellular electron transfer mechanism. Enrichments were started from anoxic corrosion products and yielded Acetobacterium as main homoacetogenic genus. Read More

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November 2018
6 Reads

Microbial subnetworks related to short-term diel O2 fluxes within geochemically distinct freshwater wetlands.

FEMS Microbiol Lett 2018 Dec;365(24)

Institute for Great Lakes Research, CMU Biological Station, and Department of Biology, Central Michigan University, Mt. Pleasant, MI 48859, USA.

Oxygen (O2) concentrations often fluctuate over diel timescales within wetlands, driven by temperature, sunlight, photosynthesis and respiration. These daily fluxes have been shown to impact biogeochemical transformations (e.g. Read More

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December 2018
2 Reads

The unbearable ease of expression-how avoidance of spurious transcription can shape G+C content in bacterial genomes.

Authors:
Uri Gophna

FEMS Microbiol Lett 2018 Dec;365(23)

Molecular Cell Biology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Levanon Street, Tel Aviv 69000, Israel.

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December 2018
4 Reads

Scientific communication strategies of microbiologists in the era of social media.

FEMS Microbiol Lett 2018 Dec;365(23)

Division of Microbial Ecology, Department of Microbiology and Ecosystem Science, Althanstrasse 14, 1090 Vienna, Austria.

Over the last decades, the world of communication underwent drastic changes, and internet and social media emerged as essential vehicles for exchanging information. Following these trends, it is important that scientists adapt to changes and adopt optimal strategies to communicate with colleagues, lay people and institutions. We conducted an online survey to investigate the communication strategies of microbiologists and their colleagues from other disciplines. Read More

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December 2018
5 Reads

A rapid and non-pathogenic assay for association of Mycobacterium tuberculosis gyrBA mutations and fluoroquinolone resistance using recombinant Mycobacterium smegmatis.

FEMS Microbiol Lett 2018 Dec;365(23)

Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, Tokyo, Japan.

We developed a method involving recombinant Mycobacterium bovis bacillus Calmette-Guérin (BCG) and recombinant Mycobacterium smegmatis to determine which mutations in Mycobacterium tuberculosis (Mtb) gyrBA are associated with fluoroquinolone (FQ) resistance. The minimal inhibitory concentration (MIC) for FQ for recombinant strains with wild-type Mtb gyrBA was equivalent to that for strains with intrinsic gyrBA. Among 27 gyrBA mutations, the fold-changes in FQ MIC for M. Read More

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December 2018

Pharmacological inhibition of pigmentation in Cryptococcus.

FEMS Yeast Res 2019 Jan;19(1)

Instituto de Microbiologia Paulo de Góes (IMPG), Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.

Melanin formation is a promising target for antifungal development. We screened a collection of 727 compounds that were previously approved for clinical use in humans for inhibition of pigmentation in Cryptococcus gattii, a lethal fungal pathogen that causes damage to both immunocompetent and immunocompromised hosts. The pyrimidine analogues flucytosine (5-fluorocytosine [5-FC]), 5-fluorouracil (5-FU) and carmofur were identified as efficient inhibitors of pigmentation in the C. Read More

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January 2019
5 Reads

Fluorescence In Situ Hybridization, a complementary molecular tool for the clinical diagnosis of infectious diseases by intracellular and fastidious bacteria.

Authors:
E Prudent D Raoult

FEMS Microbiol Rev 2018 Nov 10. Epub 2018 Nov 10.

Aix Marseille Université, IRD, APHM, MEPHI, IHU-Me´diterrane´e Infection,19-21 Boulevard Jean Moulin, 13005 Marseille, France.

Many obligate or facultative intracellular bacteria pose a critical problem in clinical microbiology diagnosis as a result of their fastidious growth or lack of growth in conventional culture media. Molecular diagnosis is based on the analysis and demonstration of nucleic acids (DNA and RNA). In the field of infectiology, it combines laboratory medicine with the technology of molecular genetics to identify infectious pathogens. Read More

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November 2018

Bacterial diversity in the marine sponge Halichondria panicea from Icelandic waters and host-specificity of its dominant symbiont "Candidatus Halichondribacter symbioticus".

FEMS Microbiol Ecol 2019 Jan;95(1)

Microbiology Group, Department of Research and Innovation, Matís ohf., Vinlandsleid 12, 113 Reykjavik, Iceland.

Marine sponges can harbour diverse bacteria that contribute to host metabolism and defence. Identifying these stable members of sponge bacterial communities remains a necessary step in understanding their ecological roles and underlying co-evolutionary processes. In this study, we applied high-throughput sequencing of 16S rRNA gene amplicons, ribosomal nucleotide variant analysis and fluorescence in situ hybridisation to characterise the core members of the bacterial community in the marine sponge Halichondria panicea from Icelandic waters. Read More

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January 2019

Erg6 gene is essential for stress adaptation in Kluyveromyces lactis.

FEMS Microbiol Lett 2018 Dec;365(23)

Faculty of Sciences, Department of Microbiology and Virology, Comenius University in Bratislava, Ilkovicova 6, 842 15 Bratislava, Slovakia.

We investigated the effect of Kluyveromyces lactis ERG6 gene deletion on plasma membrane function and showed increased susceptibility of mutant cells to salt stress, cationic drugs and weak organic acids. Contrary to Saccharomyces cerevisiae, Klerg6 mutant cells exhibited increased tolerance to tunicamycin. The content of cell wall polysacharides did not significantly vary between wild-type and mutant cells. Read More

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December 2018
4 Reads

Genome-wide transcriptional response to altered levels of the Rpb7 subunit of RNA polymerase II identifies its role in DNA damage response in Schizosaccharomyces pombe.

FEMS Yeast Res 2019 Jan;19(1)

University School of Biotechnology, G.G.S. Indraprastha University, Sector 16C, Dwarka, New Delhi 110078, India.

Transcription of protein-coding genes is a highly regulated process. In eukaryotes, it involves cross-talk between the RNA polymerase II enzyme and different proteins of the transcriptional machinery. Twelve different subunits, Rpb1 to Rpb12, constitute RNA polymerase II. Read More

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January 2019
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