56,885 results match your criteria FEBS letters[Journal]


Table of Contents.

Authors:

FEBS J 2018 Dec;285(24):4695

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December 2018

TRB1 negatively regulates gluconeogenesis by suppressing the transcriptional activity of FOXO1.

FEBS Lett 2018 Dec 17. Epub 2018 Dec 17.

Department of Cell Signaling, Graduate School of Pharmaceutical Sciences, Nagoya City University, 467-8603, Nagoya, Japan.

TRB1 is the mammalian ortholog of Tribbles, which controls cell division and migration during development in Drosophila. TRB1 is a pseudokinase and functions as a scaffold protein. Recent findings suggest that TRB1 plays important roles in hepatic lipid metabolism and participates in insulin resistance. Read More

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December 2018

Modulation of ABA responses by the protein kinase WNK8.

FEBS Lett 2018 Dec 16. Epub 2018 Dec 16.

Heidelberg University, Centre for Organismal Studies, Dep. Cell Biology, Im Neuenheimer Feld 230, 69120, Heidelberg, Germany.

Abscisic acid (ABA) regulates growth- and developmental processes in response to limiting water conditions. ABA functions through a core signaling pathway consisting of PYR1/PYL/RCAR ABA receptors, type 2C protein phosphatases (PP2Cs) and SnRK2-type protein kinases. Other signaling modules might converge with ABA signals through the modulation of core ABA signaling components. Read More

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December 2018

Tumor Necrosis Factor Related Apoptosis Inducing Ligand reduces the expression of the neuroprotective Na /Ca exchanger isoform NCX3 in human neuroblastoma SH-SY5Y cells.

FEBS J 2018 Dec 15. Epub 2018 Dec 15.

Department of Biomedical and Biotechnological Sciences, Section of Pharmacology, University of Catania School of Medicine, Catania, Italy.

Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL), a cytokine belonging to the TNF superfamily, is regarded as a mediator of neurotoxicity. The constitutively expressed ion exchanger NCX3 has been shown to protect neurons from injury. Its expression is induced by Nerve growth factor (NGF) through activation of its tyrosine kinase receptor trkA. Read More

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December 2018

Characterization of two steroid hydroxylases from different Streptomyces spp. and their ligand-bound and -unbound crystal structures.

FEBS J 2018 Dec 15. Epub 2018 Dec 15.

Department of Life Science and Biochemical Engineering, Sunmoon University, Asansi, 31460, Republic of Korea.

Bacterial cytochrome P450 (CYP) enzymes are involved in the hydroxylation of various endogenous substrates while using a heme molecule as a cofactor. CYPs have gained biotechnological interest as useful biocatalysts capable of altering chemical structures by adding a hydroxyl group in a regiospecific manner. Here, we identified, purified, and characterized two CYP154C4 proteins from Streptomyces sp. Read More

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December 2018

The structure of mammalian β-mannosidase provides insight into β-mannosidosis and Nystagmus.

FEBS J 2018 Dec 15. Epub 2018 Dec 15.

Department of Biochemistry and Groupe de Recherche Axé sur la Structure des Protéines, McGill University, Montreal, QC, H3G 0B1, Canada.

β-mannosidase is a lysosomal enzyme from the glycosyl hydrolase family 2 that cleaves the single β(1-4)-linked mannose at the non-reducing end of N-glycosylated proteins, and plays an important role in the polysaccharide degradation pathway. Mutations in the MANBA gene, which encodes the β-mannosidase, can lead to the lysosomal storage disease β-mannosidosis, as well as nystagmus, an eye condition characterized by involuntary eye movements. Here, we present the first structures of a mammalian β-mannosidase in both the apo and mannose-bound forms. Read More

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December 2018
1 Read

Writing a great abstract: tips from an Editor.

Authors:
Daniela Ruffell

FEBS Lett 2018 Dec 15. Epub 2018 Dec 15.

FEBS Letters Editorial Office, Heidelberg University Biochemistry Centre, Germany.

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December 2018

Jim's view: analog to digital conversion in biology.

Authors:
James E Rothman

FEBS Lett 2018 Dec 15. Epub 2018 Dec 15.

Department of Cell Biology, Yale University School of Medicine, New Haven, CT, USA.

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December 2018

Beyond the Message: Advantages of Snapshot Proteomics with Single-Cell Mass Cytometry in Solid Tumors.

FEBS J 2018 Dec 13. Epub 2018 Dec 13.

Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN, USA.

Single-cell technologies that can quantify features of individual cells within a tumor are critical for treatment strategies aiming to target cancer cells while sparing or activating beneficial cells. Given that key players in protein networks are often the primary targets of precision oncology strategies, it is imperative to transcend the nucleic acid message and read cellular actions in human solid tumors. Here, we review the advantages of single-cell mass cytometry. Read More

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December 2018

PTP1B markedly promotes breast cancer progression and is regulated by miR-193a-3p.

FEBS J 2018 Dec 7. Epub 2018 Dec 7.

State Key Laboratory of Pharmaceutical Biotechnology, Collaborative Innovation Center of Chemistry for Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences (NAILS), School of life sciences, Nanjing University, 163 Xianlin Road, Nanjing, Jiangsu, 210023, China.

The protein tyrosine phosphatase PTP1B, which is encoded by PTPN1, is a ubiquitously expressed non-receptor protein tyrosine phosphatase. PTP1B has long been known to negatively regulate insulin and leptin receptor signalling. Recently, it was reported to be aberrantly expressed in cancer cells and to function as an important oncogene. Read More

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December 2018

Cardiomyocyte calcineurin is required for the onset and progression of cardiac hypertrophy and fibrosis in adult mice.

FEBS J 2018 Dec 7. Epub 2018 Dec 7.

Gene regulation in cardiovascular remodeling and inflammation group, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain.

Previous studies have demonstrated that activation of calcineurin induces pathological cardiac hypertrophy. In these studies, loss-of-function was mostly achieved by systemic administration of the calcineurin inhibitor cyclosporin A. The lack of conditional knockout models for calcineurin function has impeded progress toward defining the role of this protein during the onset and the development of cardiac hypertrophy in adults. Read More

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December 2018

Elucidation of the D-lysine biosynthetic pathway in the hyperthermophile Thermotoga maritima.

FEBS J 2018 Dec 7. Epub 2018 Dec 7.

Graduate School of Pharmaceutical Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo, 108-8641, Japan.

Various D-amino acids are involved in peptidoglycan and biofilm metabolism in bacteria, suggesting that these compounds are necessary for successful adaptation to environmental changes. In addition to the conventional D-alanine (D-Ala) and D-glutamate, the peptidoglycan of the hyperthermophilic bacterium Thermotoga maritima contains both L-lysine (L-Lys) and D-Lys, but not meso-diaminopimelate (meso-Dpm). D-Lys is an uncommon component of peptidoglycan, and its biosynthetic pathway remains unclear. Read More

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December 2018

Ice-binding proteins from the fungus Antarctomyces psychrotrophicus possibly originate from two different bacteria through horizontal gene transfer.

FEBS J 2018 Dec 10. Epub 2018 Dec 10.

Graduate School of Life Science, Hokkaido University, Sapporo, 060-0810, Japan.

Various microbes, including fungi and bacteria, that live in cold environments produce ice-binding proteins (IBPs) that protect them from freezing. Ascomycota and Basidiomycota are two major phyla of fungi, and Antarctomyces psychrotrophicus is currently designated as the sole ascomycete that produces IBP (AnpIBP). However, its complete amino acid sequence, ice-binding property, and evolutionary history have not yet been clarified. Read More

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December 2018

Sulforaphane-induced metabolomic responses with epigenetic changes in estrogen receptor positive breast cancer cells.

FEBS Open Bio 2018 Dec 14;8(12):2022-2034. Epub 2018 Nov 14.

Department of Hygienic Analysis and Detection and Ministry of Education Key Lab for Modern Toxicology School of Public Health Nanjing Medical University China.

Estrogen is a risk factor for breast cancer. The isothiocyanate sulforaphane (SFN), found in cruciferous vegetables, has been identified as an effective chemopreventive agent, and may prevent or treat breast cancer by reversing estrogen-induced metabolic changes. Here, we investigated metabolic changes in estrogen receptor-positive breast cancer (MCF-7) cells treated with estradiol (E) and/or SFN to identify key metabolite panels that might provide new insights into the underlying mechanisms of the antitumor effects of SFN. Read More

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December 2018

Inhibitors of BMP-1/tolloid-like proteinases: efficacy, selectivity and cellular toxicity.

FEBS Open Bio 2018 Dec 12;8(12):2011-2021. Epub 2018 Nov 12.

Tissue Biology and Therapeutic Engineering Unit (LBTI) UMR5305, CNRS Univ Lyon Université Claude Bernard Lyon1 France.

BMP-1/tolloid-like proteinases belong to the astacin family of human metalloproteinases, together with meprins and ovastacin. They represent promising targets to treat or prevent a wide range of diseases such as fibrotic disorders or cancer. However, the study of their pathophysiological roles is still impaired by the lack of well-characterized inhibitors and the questions that remain regarding their selectivity and efficiency. Read More

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December 2018
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Human nitrobindin: the first example of an all-β-barrel ferric heme-protein that catalyzes peroxynitrite detoxification.

FEBS Open Bio 2018 Dec 9;8(12):2002-2010. Epub 2018 Nov 9.

Interdepartmental Laboratory for Electron Microscopy Roma Tre University Italy.

Nitrobindins (Nbs), constituting a heme-protein family spanning from bacteria to , display an all-β-barrel structural organization. Human Nb has been described as a domain of the nuclear protein named THAP4, whose physiological function is still unknown. We report the first evidence of the heme-Fe(III)-based detoxification of peroxynitrite by the all-β-barrel -terminal Nb-like domain of THAP4. Read More

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December 2018

Nephronectin mediates p38 MAPK-induced cell viability via its integrin-binding enhancer motif.

FEBS Open Bio 2018 Dec 15;8(12):1992-2001. Epub 2018 Nov 15.

Department of Clinical and Molecular Medicine Faculty of Medicine and Health Sciences Norwegian University of Science and Technology (NTNU) Trondheim Norway.

Nephronectin (NPNT) is an extracellular matrix (ECM) protein involved in kidney development. We recently reported intracellular NPNT as a potential prognostic marker in breast cancer and that NPNT promotes metastasis in an integrin-dependent manner. Here, we used reverse-phase protein array (RPPA) to analyze NPNT-triggered intracellular signaling in the 66cl4 mouse breast cancer cell line. Read More

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December 2018

Establishment and characterization of a novel vincristine-resistant diffuse large B-cell lymphoma cell line containing the 8q24 homogeneously staining region.

FEBS Open Bio 2018 Dec 20;8(12):1977-1991. Epub 2018 Nov 20.

Division of Hematology Department of Internal Medicine Aichi Medical University Japan.

Chromosome band 8q24 is the most frequently amplified locus in various types of cancers. has been identified as the primary oncogene at the 8q24 locus, whereas a long noncoding gene, , which lies adjacent to , has recently emerged as another potential oncogenic regulator at this position. In this study, we established and characterized a novel cell line, AMU-ML2, from a patient with diffuse large B-cell lymphoma (DLBCL), displaying homogeneously staining regions at the 8q24 locus. Read More

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December 2018

Critical role of tristetraprolin and AU-rich element RNA-binding protein 1 in the suppression of cancer cell growth by globular adiponectin.

FEBS Open Bio 2018 Dec 12;8(12):1964-1976. Epub 2018 Nov 12.

College of Pharmacy Yeungnam University Gyeongsan Korea.

Adiponectin exhibits potent antitumor activities. Herein, we examined the molecular mechanisms underlying suppression of tumor growth by globular adiponectin (gAcrp). We demonstrated that gAcrp suppressed B-cell lymphoma 2 (Bcl-2) expression, an anti-apoptotic gene, by inducing its mRNA destabilization, which was accompanied with a decrease in cell viability and increased caspase-3 activity in hepatic cancer cells. Read More

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December 2018

Interleukin-18 expression in oral squamous cell carcinoma: its role in tumor cell migration and invasion, and growth of tumor cell xenografts.

FEBS Open Bio 2018 Dec 31;8(12):1953-1963. Epub 2018 Oct 31.

Department of Oral and Maxillofacial Surgery School and Hospital of Stomatology Jilin University Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling Changchun Jilin China.

Oral squamous cell carcinoma (OSCC) is one of the most common head and neck malignancies. Advanced stages of the disease are associated with poor survival, highlighting a need for new treatment modalities. We previously showed that the proinflammatory cytokine interleukin-18 (IL-18) has a tumor suppressive role in OSCC. Read More

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December 2018
1 Read

ZNF121 interacts with ZBRK1 and BRCA1 to regulate their target genes in mammary epithelial cells.

FEBS Open Bio 2018 Dec 8;8(12):1943-1952. Epub 2018 Nov 8.

Department of Genetics and Cell Biology Nankai University College of Life Sciences Tianjin China.

The novel zinc finger protein 121 (ZNF121) has been demonstrated to physically and functionally associate with the MYC oncoprotein to regulate cell proliferation and likely breast cancer development. To further understand how ZNF121 functions in cell proliferation and carcinogenesis, we identified and characterized the interaction of ZNF121 with zinc finger and BRCA1-interacting protein with a KRAB domain 1 (ZBRK1), a breast and ovarian cancer susceptibility protein 1 (BRCA1)-interacting protein, using the yeast two-hybrid assay and other approaches. We also found that ZNF121 bound to BRCA1. Read More

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December 2018

Transforming growth factor-β blocks glucose-induced inflammation and apoptosis in corneal epithelial cells.

FEBS Open Bio 2018 Dec 6;8(12):1936-1942. Epub 2018 Nov 6.

Department of Ophthalmology Zoucheng People's Hospital China.

Diabetic retinopathy is the most important ocular complication associated with diabetes. Corneal defects due to diabetes mellitus (DM) may cause severe vision impairments. This study aimed to identify the effect of transforming growth factor-β (TGF-β) on biological events, such as apoptosis and inflammation, in the diabetic cornea. Read More

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December 2018

Quantitative analysis of sensitivity to a Wnt3a gradient in determination of the pole-to-pole axis of mitotic cells by using a microfluidic device.

FEBS Open Bio 2018 Dec 9;8(12):1920-1935. Epub 2018 Nov 9.

Department of Biosciences and Informatics Keio University Yokohama Japan.

Proper determination of the cell division axis is essential during development. Wnt3a is a known regulator of the cell division axis; however, the sensitivity of cells to Wnt3a signalling and its role in determining the cell division axis have not been measured to date. To address this gap, we took advantage of the asymmetric distribution of outer dense fibre 2 (ODF2/cenexin) proteins on centrosomes in dividing cells. Read More

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December 2018

Trastuzumab induces PUMA-dependent apoptosis and inhibits tumor growth in gastric cancer.

FEBS Open Bio 2018 Dec 19;8(12):1911-1919. Epub 2018 Nov 19.

Department of Radiology Shanghai East Hospital Tongji University School of Medicine Shanghai China.

Gastric cancer (GC) is one of the most prevalent cancers worldwide. Trastuzumab has been approved for the treatment of metastatic GC, gastroesophageal junction cancer, and breast cancer. However, the mechanisms involved in trastuzumab-induced GC cell apoptosis remain largely unknown. Read More

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December 2018

The triheme cytochrome PpcF from exhibits distinct redox properties.

FEBS Open Bio 2018 Dec 8;8(12):1897-1910. Epub 2018 Nov 8.

UCIBIO-Requimte Departamento de Química Faculdade de Ciências e Tecnologia Universidade NOVA de Lisboa Caparica Portugal.

Electrogenic bacteria, such as , can couple the oxidation of carbon sources to the reduction of extracellular electron acceptors; such acceptors include toxic and radioactive metals, as well as electrode surfaces, making a suitable candidate for applied use in bioremediation and bioenergy generation. is more promising in this regard than the better studied , as it has more efficient Fe (III) reduction rates and can convert nitrate to ammonia. The operon responsible for nitrate reductase activity in includes the gene encoding the cytochrome PpcF, which was proposed to exchange electrons with nitrate reductase. Read More

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December 2018

Potential role of platelets for atherosclerotic events in rheumatoid arthritis.

FEBS Open Bio 2018 Dec 6;8(12):1888-1896. Epub 2018 Nov 6.

Center for Gender-Specific Medicine Biomarkers Unit Istituto Superiore di Sanità Rome Italy.

Rheumatoid arthritis (RA) is a chronic inflammatory disease with increased risk of cardiovascular events and mortality that can be attributed to accelerated atherosclerosis. This pilot study aimed to investigate if changes in blood parameters were compatible with atherosclerotic events in RA patients. To this aim, 45 RA women (aged more than 18 years), and 25 age and gender-matched healthy donors (HD) were included. Read More

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December 2018

Novel approach to improve progesterone hydroxylation selectivity by CYP106A2 via rational design of adrenodoxin binding.

FEBS J 2018 Dec 7. Epub 2018 Dec 7.

Department of Biochemistry, Saarland University, Campus B2.2, 66123, Saarbrücken, Germany.

Bacterial P450s have considerable potential for biotechnological applications. The P450, CYP106A2, from Bacillus megaterium ATCC 13368 converts progesterone to several hydroxylated products that are important precursors for pharmaceutical substances. As high yields of monohydroxylated products are required for biotechnological processes, improving this conversion is of considerable interest. Read More

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December 2018

Structural and mechanistic insights into polymyxin resistance mediated by EptC originating from Escherichia coli.

FEBS J 2018 Dec 7. Epub 2018 Dec 7.

Division of Respiratory and Critical Care Medicine, State Key Laboratory of Biotherapy, West China Hospital of Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, 610041, China.

Gram-negative bacteria defend against the toxicity of polymyxins by modifying their outer membrane lipopolysaccharide (LPS). This modification mainly occurs through the addition of cationic molecules such as phosphoethanolamine (PEA). EcEptC is a PEA transferase from Escherichia coli (E. Read More

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December 2018
4.001 Impact Factor

New horizons in Glycobiology research.

Authors:
Sandro Sonnino

FEBS Lett 2018 Dec;592(23):3771-3772

Department of Medical Biotechnology and Translational Medicine, University of Milan, Italy.

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December 2018

Dual role of the σ factor in primer RNA synthesis by bacterial RNA polymerase.

FEBS Lett 2018 Dec 8. Epub 2018 Dec 8.

Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182, Russia.

Bacterial RNA polymerase (RNAP) serves as a primase during replication of single-stranded plasmids and filamentous phages. Primer RNA (prRNA) synthesis from the origin regions of these replicons depends on the σ factor that normally participates in promoter recognition. However, it was proposed that σ may not be required for origin recognition but is rather involved in RNA extension by RNAP. Read More

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December 2018
1 Read

Time-resolved Interaction Proteomics of the GIGANTEA Protein Under Diurnal Cycles in Arabidopsis.

FEBS Lett 2018 Dec 7. Epub 2018 Dec 7.

SynthSys and School of Biological Sciences, C. H. Waddington Building, University of Edinburgh, King's Buildings, Edinburgh, EH9 3BF, United Kingdom.

The plant-specific protein GIGANTEA (GI) controls many developmental and physiological processes, mediating rhythmic post-translational regulation. GI physically binds several proteins implicated in the circadian clock, photoperiodic flowering, and abiotic stress responses. To understand GI's multifaceted function, we aimed to comprehensively and quantitatively identify potential interactors of GI in a time-specific manner, using proteomics on Arabidopsis plants expressing epitope-tagged GI. Read More

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December 2018

Cis-trans proline isomers in the catalytic domain of calcineurin.

FEBS J 2018 Dec 7. Epub 2018 Dec 7.

BioNMR Laboratory, Inorganic and Organic Chemistry Department, University of Barcelona, Baldiri Reixac 10-12, 08028, Barcelona, Spain.

Calcineurin is an essential calcium activated serine/threonine phosphatase. The six NMR-observable methionine methyl groups in the catalytic domain of human calcineurin Aα (CNA) were assigned and used as reporters of the presence of potential cis-trans isomers in solution. Proline 84 is found in the cis conformation in most calcineurin X-ray structures, and proline 309, which is part of a highly conserved motif in phosphoprotein phosphatases, was modeled with a cis-peptide bond in one of the two molecules present in the asymmetric unit of CNA. Read More

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December 2018
1 Read

Identification of temperature-sensitive mutations and characterization of thermolabile RNase II variants.

FEBS Lett 2018 Dec 8. Epub 2018 Dec 8.

Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.

The RNase II family of ribonucleases is ubiquitous and critical for RNA metabolism. The rnb500 allele has been widely used for over 30 years; however, the underlying genetic changes which result in RNase II thermolabile activity remain unknown. Here, we combine molecular and biophysical studies to carry out an in vivo and in vitro investigation of RNase II mutation(s) that confer the rnb500 phenotype. Read More

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December 2018

Previously uncharacterized amino acid residues in histone H3 and H4 mutants with roles in DNA damage repair response and cellular aging.

FEBS J 2018 Dec 7. Epub 2018 Dec 7.

Laboratory of Chromatin Biology, Department of Biological Sciences, Indian Institute of Science Education and Research, Bhopal- 462023, India.

Chromatin regulates gene expression and genome maintenance, and consists of histones and other components. The post translational modification of histones plays a key role in maintaining the structure and function of chromatin under different pathophysiological stress conditions. Here, we investigate the functions of previously unexplored amino acid residues in histones H3 and H4. Read More

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December 2018
2 Reads

Engagement of Fas differentially regulates the production of LPS-induced pro-inflammatory cytokines and type I Interferons.

FEBS J 2018 Dec 11. Epub 2018 Dec 11.

Departments of Pathology, University College Cork, Cork, Ireland.

Fas (CD95) signalling is best known for its role in apoptosis, however, recent reports have shown it to be involved in other cellular responses as well, including inflammation. Fas and its adaptor protein FADD are known to negatively regulate LPS-induced pro-inflammatory responses, but their role in LPS-induced type I Interferon production is unknown. Here, we demonstrate that Fas engagement on macrophages, using an agonistic Fas antibody CH11, augments LPS-induced NF-κB responses, causing increased production of TNFα, IL-8, IL-6 and IL-12. Read More

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December 2018

Structural basis for neutralization of cytotoxic abrin by monoclonal antibody D6F10.

FEBS J 2018 Dec 6. Epub 2018 Dec 6.

Department of Physics, Indian Institute of Science, Bengaluru, 560012, India.

Abrin, an extremely cytotoxic Type-II ribosome inactivating protein (RIP), is a potential bio-warfare agent. Abrin A-chain (ABA) depurinates an adenosine of sarcin-ricin loop (SRL) from eukaryotic 28S rRNA thereby arresting protein synthesis and leading to cell death. Monoclonal antibody (mAb) D6F10 is the only known antibody that neutralizes ABA's activity in cell-free systems as well as abrin's toxicity in vitro and in vivo. Read More

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December 2018
2 Reads

Interleukin-32ε induces caspase-independent apoptosis mediated by N-Myc interactor in macrophages infected with Mycobacterium tuberculosis.

FEBS J 2018 Dec 6. Epub 2018 Dec 6.

Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Mycobacterium tuberculosis (Mtb) is the pathogen responsible for tuberculosis, a leading cause of illness and death worldwide. Growing evidence suggests that the proinflammatory cytokine IL-32 plays a major role in host defenses against pathogens such as Mtb. IL-32 exists in six alternatively spliced isoforms, but anti-tuberculosis effects have been reported only for some of them. Read More

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December 2018
1 Read

The inner ear proteome of fish.

FEBS J 2018 Dec 6. Epub 2018 Dec 6.

The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville Campus, Victoria, Australia, 3010.

The mechanisms that underpin the formation, growth and composition of otoliths, the biomineralized stones in the inner ear of fish, are largely unknown, as only a few fish inner ear proteins have been reported. Using a partial transcriptome for the inner ear of black bream (Acanthopagrus butcheri), in conjunction with proteomic data, we discovered hundreds of previously unknown proteins in the otolith. This allowed us to develop hypotheses to explain the mechanisms of inorganic material supply and daily formation of growth bands. Read More

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December 2018
2 Reads

TDP-43 accelerates deadenylation of target mRNAs by recruiting Caf1 deadenylase.

FEBS Lett 2018 Dec 6. Epub 2018 Dec 6.

Department of Biological Chemistry, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, 467-8603, Japan.

TAR DNA-binding protein 43 (TDP-43) is an RNA-binding protein, whose loss-of-function mutation causes amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). Recent studies demonstrated that TDP-43 binds to the 3' UTR of target mRNAs to promote mRNA instability. Here, we show that TDP-43 recruits Caf1 deadenylase to mRNA targets and accelerates their deadenylation. Read More

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December 2018
1 Read

Table of Contents.

Authors:

FEBS J 2018 Dec;285(23):4512

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December 2018

Crystal structure of Kumaglobin: a hexacoordinated heme protein from an anhydrobiotic tardigrade, Ramazzottius varieornatus.

FEBS J 2018 Dec 1. Epub 2018 Dec 1.

Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.

Tardigrades, also known as water bears, can survive extreme conditions. For example, tardigrades have high tolerance to extreme desiccation because they can enter an anhydrobiotic state, in which they show no or nearly undetectable metabolic processes. Proteins from anhydrobiotic tardigrades with low homology to known proteins from other organisms are new potential targets for structural genomics. Read More

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December 2018
3 Reads

To the edge of cell death and back.

FEBS J 2018 Dec 1. Epub 2018 Dec 1.

Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.

Programmed cell death plays a central role in maintaining homeostasis. Various studies have demonstrated that programmed cell death is not a one-way street; cells can survive even when the core cell death processes are underway. Cell death initiation, prevention, and recovery function in a coordinated fashion to establish and maintain a homeostatic environment. Read More

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December 2018

The mechanosensitive Piezo1 channel: a three-bladed propeller-like structure and a lever-like mechanogating mechanism.

FEBS J 2018 Nov 30. Epub 2018 Nov 30.

State Key Laboratory of Membrane Biology, School of Pharmaceutical Sciences, Tsinghua University, Beijing, China.

The evolutionarily conserved Piezo proteins, including Piezo1 and Piezo2, constitute a bona fide class of mechanosensitive (MS) cation channels, which play critical roles in various mammalian physiologies, including sensation of touch, proprioception and regulation of vascular development, and blood pressure. Furthermore, mutations in Piezos have been linked to various human genetic diseases, validating their potential as therapeutic targets. Thus, it is pivotal to understand how Piezo channels effectively convert mechanical force into selective cation permeation, and therefore precisely control the various mechanotransduction processes. Read More

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November 2018

p62/SQSTM1: 'Jack of all trades' in health and cancer.

FEBS J 2018 Nov 30. Epub 2018 Nov 30.

Department of Biochemistry, Niigata University Graduate School of Medical and Dental Sciences, Chuo-ku, Niigata, 951-8510, Japan.

p62 is a stress-inducible protein able to change among binding partners, cellular localizations and form liquid-droplet structures in a context-dependent manner. This protein is mainly defined as a cargo receptor for selective autophagy, a process that allows the degradation of detrimental and unnecessary components through the lysosome. Besides this role, its ability to interact with multiple binding partners allows p62 to act as a main regulator of the activation of the Nrf2, mTORC1 and NF-κB signaling pathways, linking p62 to the oxidative defense system, nutrient sensing and inflammation, respectively. Read More

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November 2018

Stereoselective catalysis controlled by a native leucine or variant isoleucine wing-gatekeeper in 2-haloacid dehalogenase.

FEBS Lett 2018 Nov 30. Epub 2018 Nov 30.

Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.

Comprehensively understanding enzymatic stereoselectivity will assist in the creation of new enzymes for producing optically pure compounds for chemical applications. The essential features for selecting enantiomers are controlled by particular residues or regions of the enzymes. We report a stereoselective mechanism in the D-2-haloacid dehalogenase HadD AJ1, in which L288 is identified as a gatekeeper in the access channel that strictly recognizes D-enantiomers. Read More

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November 2018
3 Reads

The flavonoid baicalin improves glucose metabolism by targeting the PH domain of AKT and activating AKT/GSK3β phosphorylation.

FEBS Lett 2018 Nov 29. Epub 2018 Nov 29.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, China.

Baicalin is one of the main flavonoids of the dried root of Scutellaria baicalensis Georgi and is reported to exert beneficial effects on the regulation of glucose/lipid metabolism. However, understanding its specific target and unique mechanism for improving glucose utilization is a challenge. In this paper, target fishing with a baicalin probe reveals that baicalin interacts with AKT. Read More

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November 2018
6 Reads
3.169 Impact Factor

Novel functions of peroxiredoxin Q from Deinococcus radiodurans R1 as a peroxidase and a molecular chaperone.

FEBS Lett 2018 Nov 29. Epub 2018 Nov 29.

Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Korea.

Deinococcus radiodurans R1 is extremely resistant to ionizing radiation and oxidative stress. In this study, we characterized DR0846, a candidate peroxiredoxin in D. radiodurans. Read More

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November 2018
3.169 Impact Factor

Rab5 activation on macropinosomes requires ALS2, and subsequent Rab5 inactivation through ALS2 detachment requires active Rab7.

FEBS Lett 2018 Nov 28. Epub 2018 Nov 28.

Division of Biosignaling, Research Institute for Biomedical Sciences, Tokyo University of Science, Noda, Japan.

Macropinocytosis is a nonspecific bulk uptake of extracellular fluid. During endosome maturation, the Rab5-to-Rab7 switch machinery executes the conversion from early to late endosomes. However, how the Rab switch works during macropinosome maturation remains unclear. Read More

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November 2018
1 Read

Environmental pH and Glu364 to Gln mutation in the chlorophyll-binding CP47 protein affect redox-active TyrD and charge recombination in Photosystem II.

FEBS Lett 2018 Nov 28. Epub 2018 Nov 28.

Department of Biochemistry, University of Otago, Dunedin, New Zealand.

In Photosystem II, loop E of the chlorophyll-binding CP47 protein is located near a redox-active tyrosine, Y , forming a symmetrical analog to loop E in CP43, which provides a ligand to the oxygen-evolving complex (OEC). A Glu364 to Gln substitution in CP47, near Y , does not affect growth in the cyanobacterium Synechocystis sp. PCC 6803; however, deletion of the extrinsic protein PsbV in this mutant leads to a strain displaying a pH-sensitive phenotype. Read More

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November 2018
1 Read

The structure of Legionella effector protein LpnE provides insights into its interaction with Oculocerebrorenal syndrome of Lowe (OCRL) protein.

FEBS J 2018 Nov 26. Epub 2018 Nov 26.

Department of Biochemistry, University of Saskatchewan, 107 Wiggins Road, Saskatoon, Saskatchewan, S7N 5E5, Canada.

Legionella pneumophila is a freshwater bacterium that replicates in predatory amoeba and alveolar macrophage. The ability of L. pneumophila to thrive in eukaryotic host cells is conferred by the Legionella containing vacuole (LCV). Read More

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November 2018
1 Read
4.001 Impact Factor