48,195 results match your criteria FEBS letters[Journal]


miR-134 inhibits osteosarcoma cell invasion and metastasis through targeting MMP1 and MMP3 in vitro and in vivo.

FEBS Lett 2019 Apr 12. Epub 2019 Apr 12.

Department of Orthopaedics, Second Hospital of Shanxi Medical University, TaiYuan, 030001, China.

miR-134 has been shown to be associated with angiogenesis and the progression of osteosarcoma. This study further assessed the effects of miR-134 expression on osteosarcoma cell migration, invasion, and metastasis in vitro and in a nude mouse xenograft model, exploring the underlying molecular events. Luciferase reporter assays revealed that miR-134 directly targets the 3'-UTRs of MMP1 and MMP3 to reduce their expression in osteosarcoma cells. Read More

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http://dx.doi.org/10.1002/1873-3468.13387DOI Listing

Orphan nuclear receptor NR4A1 suppresses hyperhomocysteinemia-induced hepatic steatosis in vitro and in vivo.

FEBS Lett 2019 Apr 11. Epub 2019 Apr 11.

Department of Cell Biology and Genetics, Key Laboratory of Molecular Biology in High Cancer Incidence Coastal Chaoshan Area of Guangdong Higher Education Institutes, Shantou University Medical College, Shantou, 515041, China.

Homocysteine (Hcy) is associated with non-alcoholic fatty liver disease (NAFLD). NR4A1 is involved in hepatic lipid metabolism. However, the potential role of NR4A1 in Hcy-associated NAFLD remains elusive. Read More

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http://dx.doi.org/10.1002/1873-3468.13384DOI Listing
April 2019
2 Reads

The TRIM14 PRYSPRY domain mediates protein interaction via its basic interface.

FEBS Lett 2019 Apr 11. Epub 2019 Apr 11.

Institute of Systems Biomedicine, Department of Pathology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, P. R. China.

TRIM14 was recently shown to be an important molecule against pathogens. Its PRYSPRY domain acts as a protein-protein interaction module. TRIM14 exerts distinct functions via its PRYSPRY domain by interacting with different partners. Read More

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http://dx.doi.org/10.1002/1873-3468.13386DOI Listing
April 2019
1 Read

Domain structure of HelD, an interaction partner of Bacillus subtilis RNA polymerase.

FEBS Lett 2019 Apr 10. Epub 2019 Apr 10.

Laboratory of Structure and Function of Biomolecules, Institute of Biotechnology of the Czech Academy of Sciences, v. v. i, Biocev, Průmyslová 595, 25250, Vestec, Czech Republic.

HelD is a helicase-like protein binding to Bacillus subtilis RNA polymerase (RNAP), stimulating transcription in an ATP-dependent manner. Here, our small angle X-ray scattering data bring the first insights into the HelD structure: HelD is compact in shape and undergoes a conformational change upon substrate analog binding. Furthermore, the HelD domain structure is delineated, and a partial model of HelD is presented. Read More

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http://dx.doi.org/10.1002/1873-3468.13385DOI Listing

A Tandem Active Site Model for the Ribosomal Helicase.

FEBS Lett 2019 Apr 10. Epub 2019 Apr 10.

Center for Molecular Biology of RNA and Department of Molecular, Cell and Developmental Biology, University of California at Santa Cruz, Santa Cruz, CA, 95064, USA.

During protein synthesis, the mRNA helicase activity of the ribosome ensures that codons are made single-stranded before decoding. Here, based on recent structural and functional findings, a quantitative model is presented for a tandem arrangement of two helicase active sites on the ribosome. A distal site encounters mRNA structures first, one elongation cycle earlier than a proximal site. Read More

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http://dx.doi.org/10.1002/1873-3468.13383DOI Listing

The science of molecular gastronomy and the art of innovative cooking.

Authors:
Hervé This

FEBS Lett 2019 Apr 10. Epub 2019 Apr 10.

Ingénierie Procédés Aliments, AgroParisTech, INRA, Université Paris-Saclay, Massy, France.

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http://dx.doi.org/10.1002/1873-3468.13373DOI Listing
April 2019
1 Read

Axonemal doublet microtubules can split into two complete singlets in human sperm flagellum tips.

FEBS Lett 2019 Apr 8. Epub 2019 Apr 8.

Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.

Motile flagella are crucial for human fertility and embryonic development. The distal tip of the flagellum is where growth and intra-flagellar transport are coordinated. In most, but not all, model organisms, the distal tip includes a "singlet region", where axonemal doublet microtubules terminate and only complete A-tubules extend as singlet microtubules to the tip. Read More

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http://dx.doi.org/10.1002/1873-3468.13379DOI Listing
April 2019
1 Read

Hepatocyte nuclear factor 1 alpha (HNF1A) regulates transcription of O-GlcNAc transferase in a negative feedback mechanism.

FEBS Lett 2019 Apr 5. Epub 2019 Apr 5.

College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, 300353, China.

O-GlcNAc transferase (OGT)-catalyzed protein O-GlcNAcylation is implicated in diverse cellular events. Here, we report the regulation of OGT transcription by the hepatocyte nuclear factor 1 homologue A (HNF1A) in HEK293T cells. We firstly identified a core ogt promoter (-150 ~ + 200bp) and proved its binding to the transcription factor HNF1A. Read More

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http://dx.doi.org/10.1002/1873-3468.13381DOI Listing
April 2019
1 Read

The aryl hydrocarbon receptor is a novel negative regulator of interleukin-17-mediated signaling and inflammation in vitro.

FEBS Lett 2019 Apr 5. Epub 2019 Apr 5.

Department of Clinical Laboratory, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310005, China.

Interleukin (IL)-17 plays a critical role in the pathogenesis of inflammation and autoimmune diseases. The aryl hydrocarbon receptor (AHR) is a transcription factor and responsible for the elimination of xenobiotic chemicals. However, it remains unknown whether AHR is involved in IL-17 signaling. Read More

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http://dx.doi.org/10.1002/1873-3468.13380DOI Listing

N-Degron Specificity of Chloroplast ClpS1 in Plants.

FEBS Lett 2019 Apr 5. Epub 2019 Apr 5.

Plant Biology Section, School of Integrative Plant Sciences (SIPS), Cornell University, Emerson Hall, Ithaca, NY, 14853.

The prokaryotic N-degron pathway depends on the Clp chaperone-protease system and the ClpS adaptor for recognition of N-degron bearing substrates. Plant chloroplasts contain a diversified Clp protease, including the ClpS homolog ClpS1. Several candidate ClpS1 substrates have been identified, but the N-degron specificity is unclear. Read More

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http://dx.doi.org/10.1002/1873-3468.13378DOI Listing

Trimethylsilyl reporter groups for NMR studies of conformational changes in G protein-coupled receptors.

FEBS Lett 2019 Apr 5. Epub 2019 Apr 5.

iHuman Institute, ShanghaiTech University, 393 Middle Huaxia Road, Shanghai, 201210, China.

Large membrane proteins such as G Protein-Coupled Receptors (GPCRs) are difficult for NMR study due to severe signal overlaps and unfavorable relaxation properties. We used a trimethylsilyl (TMS) group as a reporter group for H NMR study of conformational changes in proteins, utilizing high-intensity H NMR signals near 0 ppm. The β -Adrenergic Receptor (β AR) was labeled with TMS groups at two cysteines located at the cytoplasmic ends of helices VI and VII. Read More

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http://dx.doi.org/10.1002/1873-3468.13382DOI Listing
April 2019
1 Read

N-glycome inheritance from cells to extracellular vesicles in B16 melanomas.

FEBS Lett 2019 Apr 3. Epub 2019 Apr 3.

Department of Systems Biology in Thromboregulation, Kagoshima University Graduate School of Medical and Dental Sciences, Japan.

We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs). Three mouse melanoma B16 variants with distinct metastatic potentials show similar gene expression levels and enzymatic activities of glycosyltransferases involved in N-glycosylation. All melanoma variants and EVs have nearly identical profiles of de-sialylated N-glycans. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/1873-3468.13
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http://dx.doi.org/10.1002/1873-3468.13377DOI Listing
April 2019
2 Reads

Evidence for weak interaction between phytochromes Agp1 and Agp2 from Agrobacterium fabrum.

FEBS Lett 2019 Apr 2. Epub 2019 Apr 2.

Botanical Institute, Karlsruhe Institute of Technology, Germany.

During bacterial conjugation, plasmid DNA is transferred from cell to cell. In Agrobacterium fabrum, conjugation is regulated by the phytochrome photoreceptors Agp1 and Agp2. Both contribute equally to this regulation. Read More

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http://dx.doi.org/10.1002/1873-3468.13376DOI Listing
April 2019
2 Reads

Structural basis of hypoxic gene regulation by the Rv0081 transcription factor of Mycobacterium tuberculosis.

FEBS Lett 2019 Apr 2. Epub 2019 Apr 2.

National Centre for Cell Science, SP Pune University Campus, Ganeshkhind, Pune, 411 007, INDIA.

The transcription factor Rv0081 of Mycobacterium tuberculosis controls hypoxic gene expression and acts as a regulatory hub in the latent phase of tuberculosis infection. We report here the crystal structure of Rv0081 at 2.9 Å resolution revealing that it belongs to the well-known ArsR/SmtB family proteins. Read More

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http://dx.doi.org/10.1002/1873-3468.13375DOI Listing
April 2019
3 Reads

Quantitative analysis and assessment of base composition asymmetry and gene orientation bias in bacterial genomes.

FEBS Lett 2019 Apr 3. Epub 2019 Apr 3.

Department of Physics, School of Science, Tianjin University, China.

Base composition asymmetry and gene orientation bias are two common genomic structures in bacterial genomes. Here, correlation coefficients between nucleotide disparities and coding sequence (CDS) skew have been calculated, which provides insights into their relationship from an individual genome perspective. Consequently, we find GC and RY disparities correlate significantly with CDS skew, since around 60% of the bacterial genomes under study have correlation coefficients > 0. Read More

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http://dx.doi.org/10.1002/1873-3468.13374DOI Listing
April 2019
1 Read

Synergistic effect of PCPE1 and sFRP2 on the processing of procollagens via BMP1.

FEBS Lett 2019 Apr 21;593(7):760. Epub 2019 Mar 21.

This article corrects: Synergistic effect of PCPE1 and sFRP2 on the processing of procollagens via BMP1, Volume 593, Issue 1, 119-127. Article first published 09 November 2018. https://doi. Read More

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http://dx.doi.org/10.1002/1873-3468.13363DOI Listing

Spatial organization of the transcriptional regulatory network of Saccharomyces cerevisiae.

FEBS Lett 2019 Mar 25. Epub 2019 Mar 25.

Hubei Key Laboratory of Agricultural Bioinformatics, College of Informatics, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.

A transcriptional regulatory network (TRN) is a complex network composed of all of the regulatory interactions between transcription factors and the corresponding target genes. Recently, three-dimensional (3D) genomic studies have shown that the 3D structure of the genome may influence the regulation of gene transcription, which provides us with a novel perspective. In the present study, we constructed the TRN of the budding yeast Saccharomyces cerevisiae and placed it in the context of a 3D genome model. Read More

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http://dx.doi.org/10.1002/1873-3468.13371DOI Listing

Chromatin plates in the interphase nucleus.

FEBS Lett 2019 Mar 25. Epub 2019 Mar 25.

Departament de Bioquímica i Biologia Molecular, Facultat de Biociències, Universitat Autònoma de Barcelona, Bellaterra, Spain.

Early results showed the emanation of chromatin fibers from mitotic chromosomes and nuclei swollen with water. In contrast, under metaphase ionic conditions, it was found that chromatin from mitotic chromosomes is planar and forms multilayered plates. Here, we show that in buffers containing interphase cation concentrations, the chromatin emanated from disrupted nuclei also has a planar morphology. Read More

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http://dx.doi.org/10.1002/1873-3468.13370DOI Listing

5'-End sequencing in Saccharomyces cerevisiae offers new insights into 5'-ends of tRNA and snoRNAs.

FEBS Lett 2019 Mar 25. Epub 2019 Mar 25.

The Ohio State Biochemistry Program, The Ohio State University, Columbus, OH, USA.

tRNA guanylyltransferase (Thg1) specifies eukaryotic tRNA identity by catalysing a 3'-5' non-Watson-Crick (WC) addition of guanosine to the 5'-end of tRNA . Thg1 family enzymes in Archaea and Bacteria, called Thg1-like proteins (TLPs), catalyse a similar but distinct 3'-5' addition in an exclusively WC-dependent manner. Here, a genetic system in Saccharomyces cerevisiae was employed to further assess the biochemical differences between Thg1 and TLPs. Read More

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http://dx.doi.org/10.1002/1873-3468.13364DOI Listing

Stereoselective C3-substituent modification and substrate channeling by oxidoreductase BchC in bacteriochlorophyll a biosynthesis.

FEBS Lett 2019 Mar 25. Epub 2019 Mar 25.

Graduate School of Life Sciences, Ritsumeikan University, Kusatsu, Japan.

We report the in vitro activity of recombinant BchC oxidoreductase involved in bacteriochlorophyll a biosynthesis. BchC of Rhodobacter capsulatus preferentially oxidizes 3 R-3-(1-hydroxyethyl)-chlorophyllide a and 3 R-3-(1-hydroxyethyl)-bacteriochlorophyllide a in the presence of NAD to 3-acetyl-chlorophyllide a and bacteriochlorophyllide a, respectively, leaving the unreacted 3 S-epimers. In the reverse reaction, BchC with NADH predominately produces 3 R-epimeric alcohols from the 3-acetyl-(bacterio)chlorins. Read More

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http://dx.doi.org/10.1002/1873-3468.13372DOI Listing

An in vivo protease activity assay for investigating the functions of the Escherichia coli membrane protease HtpX.

FEBS Lett 2019 Mar 22. Epub 2019 Mar 22.

Institute for Frontier Life and Medical Sciences, Kyoto University, Japan.

Escherichia coli HtpX is an M48 family zinc metalloproteinase located in the cytoplasmic membrane. Previous studies suggested that it is involved in the quality control of membrane proteins. However, its in vivo proteolytic function has not been characterized in detail, mainly because the physiological substrates have not been identified and no model substrate that allows sensitive detection of the protease activity is available. Read More

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http://dx.doi.org/10.1002/1873-3468.13368DOI Listing

Identification and characterization of glycosylation sites on Litopenaeus vannamei hemocyanin.

FEBS Lett 2019 Mar 22. Epub 2019 Mar 22.

Department of Biology, Guangdong Provincial Key laboratory of Marine Biotechnology, Shantou University, China.

The respiratory glycoprotein hemocyanin has been implicated in immune-related functions. Using lectin blotting, we show that the binding of shrimp (Litopenaeus vannamei) hemocyanin to concanavalin A decreases markedly with O-glycosidase treatment but not with PNGase F. Twelve O-glycosylation sites, three on the large hemocyanin subunit and nine on the small hemocyanin subunit (HMCs), were identified by LC-MS/MS. Read More

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http://dx.doi.org/10.1002/1873-3468.13367DOI Listing

Ribosomal mutation in helix 32 of 18S rRNA alters fidelity of eukaryotic translation start site selection.

FEBS Lett 2019 Mar 22. Epub 2019 Mar 22.

School of Biological Sciences, National Institute of Science Education and Research, Bhubaneswar, India.

The 40S ribosome plays a critical role in start codon selection. To gain insights into the role of its 18S rRNA in start codon selection, a suppressor screen was performed that suppressed the preferential UUG start codon recognition (Suppressor of initiation codon: Sui phenotype) associated with the eIF5 mutant. The C1209U mutation in helix h32 of 18S rRNA was found to suppress the Sui and Gcn (failure to derepress GCN4 expression) phenotype of the eIF5 mutant. Read More

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http://dx.doi.org/10.1002/1873-3468.13369DOI Listing

Photo-reducible plastoquinone pools in chloroplasts of Tradescentia plants acclimated to high and low light.

FEBS Lett 2019 Mar 21. Epub 2019 Mar 21.

Faculty of Physics, M.V.Lomonosov Moscow State University, Russia.

In photosynthetic systems of oxygenic type, plastoquinone (PQ) molecules are reduced by photosystem II (PSII). The turnover of PQ determines the rate of PSII operation. PQ molecules are present in surplus with respect to PSII. Read More

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http://dx.doi.org/10.1002/1873-3468.13366DOI Listing
March 2019
6 Reads

The six steps to a successful start-up: an interview with Kai Simons.

Authors:
Daniela Ruffell

FEBS Lett 2019 Apr 21;593(7):655-657. Epub 2019 Mar 21.

FEBS Letters Editorial Office, Heidelberg University Biochemistry Centre, Germany.

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http://dx.doi.org/10.1002/1873-3468.13354DOI Listing

RNA polymerase pausing at a protein roadblock can enhance transcriptional interference by promoter occlusion.

FEBS Lett 2019 Mar 20. Epub 2019 Mar 20.

Department of Molecular and Biomedical Science, School of Biological Sciences, The University of Adelaide, Adelaide, Australia.

Convergent promoters exert transcriptional interference (TI) by several mechanisms including promoter occlusion, where elongating RNA polymerases (RNAPs) block access to a promoter. Here, we tested whether pausing of RNAPs by obstructive DNA-bound proteins can enhance TI by promoter occlusion. Using the Lac repressor as a 'roadblock' to induce pausing over a target promoter, we found only a small increase in TI, with mathematical modelling suggesting that rapid termination of the stalled RNAP was limiting the occlusion effect. Read More

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http://dx.doi.org/10.1002/1873-3468.13365DOI Listing

Crystal structure of a NADPH-cytochrome P450 oxidoreductase (CYPOR) and heme oxygenase 1 fusion protein implies a conformational change in CYPOR upon NADPH/NADP binding.

FEBS Lett 2019 Mar 18. Epub 2019 Mar 18.

Department of Medical Biochemistry, Kurume University School of Medicine, Kurume, Japan.

Heme oxygenase-1 (HMOX1) catalyzes heme degradation utilizing reducing equivalents supplied from NADPH-cytochrome P450 reductase (CYPOR). Recently, we determined the complex structure of NADP -bound open-conformation stabilized CYPOR and heme-HMOX1, but the resolution was limited to 4.3 Å. Read More

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http://dx.doi.org/10.1002/1873-3468.13360DOI Listing

Arginine-95 is important for recruiting superoxide to the active site of the FerB flavoenzyme of Paracoccus denitrificans.

FEBS Lett 2019 Apr 23;593(7):697-702. Epub 2019 Mar 23.

Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.

Ferric reductase B (FerB) is a flavin mononucleotide (FMN)-containing NAD(P)H:acceptor oxidoreductase structurally close to the Gluconacetobacter hansenii chromate reductase (ChrR). The crystal structure of ChrR was previously determined with a chloride bound proximal to FMN in the vicinity of Arg101, and the authors suggested that the anionic electron acceptors, chromate and uranyl tricarbonate, bind similarly. Here, we identify the corresponding arginine residue in FerB (Arg95) as being important for the reaction of FerB with superoxide. Read More

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http://dx.doi.org/10.1002/1873-3468.13359DOI Listing

A readout of metabolic efficiency in arylamine N-acetyltransferase-deficient mice reveals minor energy metabolism changes.

FEBS Lett 2019 Mar 18. Epub 2019 Mar 18.

Université Paris Diderot, Sorbonne Paris Cité, Unité BFA, CNRS, UMR 8251, Paris, France.

Recent studies have revealed a possible link between the activities of polymorphic arylamine N-acetyltransferases (NATs) and energy metabolism. We used a Nat1/Nat2 double knockout (KO) mouse model to demonstrate that ablation of the two Nat genes is associated with modest, intermittent alterations in respiratory exchange rate. Pyruvate tolerance tests show that double KO mice have attenuated hepatic gluconeogenesis when maintained on a high-fat/high-sucrose diet. Read More

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http://dx.doi.org/10.1002/1873-3468.13357DOI Listing
March 2019
3.169 Impact Factor

Translation arrest as a protein quality control system for aberrant translation of the 3'-UTR in mammalian cells.

FEBS Lett 2019 Mar 18. Epub 2019 Mar 18.

Graduate School of Pharmaceutical Science, Tohoku University, Sendai, Japan.

Read-through or mutations of a stop codon resulting in translation of the 3'-UTR produce potentially toxic C-terminally extended proteins. However, quality control mechanisms for such proteins are poorly understood in mammalian cells. Here, a comprehensive analysis of the 3'-UTRs of genes associated with hereditary diseases identified novel arrest-inducing sequences in the 3'-UTRs of 23 genes that can repress the levels of their protein products. Read More

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http://dx.doi.org/10.1002/1873-3468.13362DOI Listing

A novel inhibitor of tumorspheres reveals the activation of the serine biosynthetic pathway upon mitochondrial inhibition.

FEBS Lett 2019 Mar 15. Epub 2019 Mar 15.

Chemical Biology Research Group, RIKEN Center for Sustainable Resource Science, Wako, Japan.

Differences in the metabolism of cancer cells or cancer stem cells (CSCs) as compared to normal cells have provided avenues to safely target cancers. To discover metabolic inhibitors of CSCs, we performed alkaline phosphatase- and tumoursphere-based drug screening using induced cancer stem cell-like cells. From the screening of a RIKEN NPDepo chemical library, we discovered NPD2381 as a novel and selective cancer-stemness inhibitor that targets mitochondrial metabolism. Read More

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http://dx.doi.org/10.1002/1873-3468.13361DOI Listing

Complex N-glycan promotes CD133 mono-ubiquitination and secretion.

FEBS Lett 2019 Apr 29;593(7):719-731. Epub 2019 Mar 29.

NHC Key Laboratory of Glycoconjugates Research, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Shanghai Medical College of Fudan University, China.

CD133 is a widely used cell surface marker of cancer stem cells that plays an important role in tumor initiation and metastasis. Increasing evidence shows that CD133 is secreted to the extracellular space. However, the underlying mechanisms of CD133 secretion remain largely unknown. Read More

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http://dx.doi.org/10.1002/1873-3468.13358DOI Listing

Molecular evolution of proteins mediating mitochondrial fission-fusion dynamics.

FEBS Lett 2019 Apr 22;593(7):703-718. Epub 2019 Mar 22.

Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai, India.

Eukaryotes employ a subset of dynamins to mediate mitochondrial fusion and fission dynamics. Here we report the molecular evolution and diversification of the dynamin-related mitochondrial proteins that drive the fission (Drp1) and the fusion processes (mitofusin and OPA1). We demonstrate that the three paralogs emerged concurrently in an early mitochondriate eukaryotic ancestor. Read More

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http://dx.doi.org/10.1002/1873-3468.13356DOI Listing

JAK/STAT signaling is involved in air sac primordium development of Drosophila melanogaster.

FEBS Lett 2019 Apr 20;593(7):658-669. Epub 2019 Mar 20.

Department of Biology and Biotechnology Center, Western Kentucky University, Bowling Green, KY, USA.

The dorsal thoracic air sacs in fruit flies (Drosophila melanogaster) are functionally and developmentally comparable to human lungs. The progenitors of these structures, air sac primordia (ASPs), invasively propagate into wing imaginal disks, employing mechanisms similar to those that promote metastasis in malignant tumors. We investigated whether Janus kinase/signal transducer and activator of transcription JAK/STAT signaling plays a role in the directed morphogenesis of ASPs. Read More

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http://dx.doi.org/10.1002/1873-3468.13355DOI Listing

LINC00473 mediates cyclin D1 expression through a balance between activation and repression signals in breast cancer cells.

FEBS Lett 2019 Apr 18;593(7):751-759. Epub 2019 Mar 18.

Hefei National Laboratory for Physical Sciences at the Microscale, Hefei, Anhui, China.

Long noncoding RNAs (lncRNAs) are critical regulators in tumorigenesis. However, their roles in breast cancer remain unclear. Here, we found that lncRNA LINC00473 is significantly upregulated in breast cancer cells. Read More

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http://dx.doi.org/10.1002/1873-3468.13353DOI Listing

Decrypting the oscillating nature of the 4'-phosphopantetheine arm in acyl carrier protein AcpM of Mycobacterium tuberculosis.

FEBS Lett 2019 Mar 8;593(6):622-633. Epub 2019 Mar 8.

Department of Biotechnology, Indian Institute of Technology, Kharagpur, India.

In Mycobacterium tuberculosis, acyl carrier protein (AcpM)-mediated fatty acid synthase type II is integral for the synthesis of mycolic acids. AcpM, designated as an atypical ACP, comprises of a putative 33 amino acid long C-terminal extension which is distinctive in nature. Here, we aimed at devising an 'easy-to-go' method for the generation of crypto-AcpM loaded with a solvatochromic probe 7-Nitrobenz-2-oxa-1,3-diazol-4-yl, which is linked to the 4'-phosphopantetheine (Ppant) prosthetic group of AcpM. Read More

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http://dx.doi.org/10.1002/1873-3468.13339DOI Listing
March 2019
1 Read

Characterizing the binding of dopamine D1-D2 receptors in vitro and in temporal and frontal lobe tissue total protein.

FEBS Lett 2019 Apr 12;593(7):732-742. Epub 2019 Mar 12.

Chemistry Department, Eastern Michigan University, Ypsilanti, MI, USA.

Dysfunction of the dopaminergic pathway is linked to numerous diseases of the nervous system. The D1-D2 receptor heteromer is known to play a role in certain neuropsychiatric disorders, such as depression. Here, we synthesized an eight amino acid residue peptide, EAARRAQE, derived from the third intracellular loop of the D2 receptor and show that the peptide binds to the D1 receptor with comparable efficiency as that of the full-length D2 receptor protein. Read More

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http://dx.doi.org/10.1002/1873-3468.13351DOI Listing
April 2019
1 Read

AaPP2C1 negatively regulates the expression of genes involved in artemisinin biosynthesis through dephosphorylating AaAPK1.

FEBS Lett 2019 Apr 9;593(7):743-750. Epub 2019 Mar 9.

Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, SWU-TAAHC Medicinal Plant Joint R&D Centre, School of Life Sciences, Southwest University, Chongqing, China.

Artemisinin is biosynthesized in Artemisia annua and widely used for the treatment of malaria. Abscisic acid (ABA)-responsive kinase 1 (AaAPK1), a member of the SnRK2 family, is involved in the regulation of artemisinin biosynthesis through the phosphorylation of AabZIP1, which directly transactivates genes involved in artemisinin biosynthesis. Through diverse assays - including yeast two-hybrid and bimolecular fluorescence complementation assays - we report that the ABA-responsive protein phosphatase AaPP2C1 physically interacts with AaAPK1. Read More

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http://dx.doi.org/10.1002/1873-3468.13350DOI Listing

Improved recombinant expression and purification of functional plant Rubisco.

FEBS Lett 2019 Mar 14;593(6):611-621. Epub 2019 Mar 14.

Chaperonin-assisted Protein Folding Group, Max Planck Institute of Biochemistry, Martinsried, Germany.

Improving the performance of the key photosynthetic enzyme Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) by protein engineering is a critical strategy for increasing crop yields. The extensive chaperone requirement of plant Rubisco for folding and assembly has long been an impediment to this goal. Production of plant Rubisco in Escherichia coli requires the coexpression of the chloroplast chaperonin and four assembly factors. Read More

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http://dx.doi.org/10.1002/1873-3468.13352DOI Listing

mRNA 5' ends targeted by cytoplasmic recapping cluster at CAGE tags and select transcripts are alternatively spliced.

FEBS Lett 2019 Apr 9;593(7):670-679. Epub 2019 Mar 9.

Center for RNA Biology, The Ohio State University, Columbus, OH, USA.

Until cytoplasmic recapping was discovered, decapping was thought to irreversibly destine an mRNA to degradation. Contradicting this idea, we readily observe mRNAs targeted by cytoplasmic capping in uncapped, yet stable forms. 5' rapid amplification of cDNA ends (RACE) shows that nearly all uncapped ends correspond to capped analysis of gene expression tags and that the recapping of ZNF207 mRNA may be restricted to a single splice isoform. Read More

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http://dx.doi.org/10.1002/1873-3468.13349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6465107PMC
April 2019
2 Reads
3.169 Impact Factor

The impact of Megf10/Drpr gain-of-function on muscle development in Drosophila.

FEBS Lett 2019 Apr 12;593(7):680-696. Epub 2019 Mar 12.

Division of Pediatric Neurology, Department of Pediatrics, University of Florida College of Medicine, Gainesville, FL, USA.

Recessive mutations in multiple epidermal growth factor-like domains 10 (MEGF10) underlie a rare congenital muscle disease known as MEGF10 myopathy. MEGF10 and its Drosophila homolog Draper (Drpr) are transmembrane receptors expressed in muscle and glia. Drpr deficiency is known to result in muscle abnormalities in flies. Read More

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http://dx.doi.org/10.1002/1873-3468.13348DOI Listing
April 2019
1 Read
3.169 Impact Factor

Will Plan S put learned societies in jeopardy?

FEBS Lett 2019 Feb;593(4):383-385

Chair, FEBS Publications Committee.

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http://dx.doi.org/10.1002/1873-3468.13333DOI Listing
February 2019
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Molecular determinants on extracellular loop domains that dictate interaction between β-arrestin and human APJ receptor.

FEBS Lett 2019 Mar 5;593(6):634-643. Epub 2019 Mar 5.

Signal Transduction Laboratory, Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai, India.

The human APJ receptor (APJR), activated by apelin isoforms, regulates cardiovascular functions and fluid homeostasis. Understanding its structure-function relationship is crucial for a comprehensive knowledge of signalling aberrations that cause several physiological disorders. Here, we demonstrate the influence of extracellular loop (ECL) domains in the mechanism of β-arrestin-mediated signalling from human APJR: Apelin system. Read More

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http://dx.doi.org/10.1002/1873-3468.13344DOI Listing
March 2019
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Possible ATP trafficking by ATP-shuttles in the olfactory cilia and glucose transfer across the olfactory mucosa.

FEBS Lett 2019 Mar 9;593(6):601-610. Epub 2019 Mar 9.

Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago, Chile.

Odor transduction in the cilia of olfactory sensory neurons involves several ATP-requiring enzymes. ATP is generated by glycolysis in the ciliary lumen, using glucose incorporated from surrounding mucus, and by oxidative phosphorylation in the dendrite. During prolonged stimulation, the cilia maintain ATP levels along their length, by unknown means. Read More

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http://dx.doi.org/10.1002/1873-3468.13346DOI Listing

Endothelin type B receptor interacts with the 78-kDa glucose-regulated protein.

FEBS Lett 2019 Mar 5;593(6):644-651. Epub 2019 Mar 5.

Department of Cellular Pharmacology, Graduate School of Medicine, Hokkaido University, Sapporo, Japan.

Endothelin (ET)-1 is involved in the vascular system, cell proliferation and apoptosis. ET receptors consist of ET type A receptor (ET R) and ET type B receptor (ET R). ET R and ET R generally exhibit opposite responses, although many exceptions exist. Read More

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http://dx.doi.org/10.1002/1873-3468.13347DOI Listing

Organophosphate hydrolase interacts with Ton components and is targeted to the membrane only in the presence of the ExbB/ExbD complex.

FEBS Lett 2019 Mar 8;593(6):581-593. Epub 2019 Mar 8.

Department of Animal Biology, School of Life Sciences, University of Hyderabad, India.

Our study aims to investigate the physiological role of organophosphate hydrolase (OPH), hitherto known for its involvement in the degradation of organophosphate insecticides and nerve agents in Sphingobium fuliginis. We find that OPH exists as part of the TonB-dependent Transport system that is involved in nutrient transport across the bacterial outer membrane. OPH interacts physically with the Ton complex components ExbD and TonB. Read More

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http://dx.doi.org/10.1002/1873-3468.13345DOI Listing
March 2019
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Transgenerational inheritance of cold temperature response in Drosophila.

FEBS Lett 2019 Mar 8;593(6):594-600. Epub 2019 Mar 8.

CSIR-Institute of Genomics and Integrative Biology, New Delhi, India.

Intergenerational inheritance of transcriptional responses induced by low temperature rearing has recently been shown in Drosophila. Besides germline inheritance, fecal transfer experiments indirectly suggested that the acquired microbiome may also have contributed to the transcriptional responses in offspring. Here, we analyze expression data on inheritance of the cold-induced effects in conjunction with previously reported transcriptomic differences between flies with a microbiota or axenic flies and provide support for a contribution of the acquired microbiome to the offspring phenotype. Read More

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http://dx.doi.org/10.1002/1873-3468.13343DOI Listing

The peroxisome biogenesis factors Pex3 and Pex19: multitasking proteins with disputed functions.

FEBS Lett 2019 Mar 5;593(5):457-474. Epub 2019 Mar 5.

Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands.

The peroxisomal membrane protein (PMP) Pex3 and its cytosolic interaction partner Pex19 have been implicated in peroxisomal membrane biogenesis. Although these peroxins have been extensively studied, no consensus has been reached yet on how they operate. Here, we discuss two major models of their function, namely, in direct insertion of proteins into the peroxisomal membrane or in formation of PMP-containing vesicles from the endoplasmic reticulum (ER). Read More

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http://dx.doi.org/10.1002/1873-3468.13340DOI Listing
March 2019
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Structural considerations of folded protein import through the chloroplast TOC/TIC translocons.

FEBS Lett 2019 Mar 5;593(6):565-572. Epub 2019 Mar 5.

Department of Plant Biology, University of California Davis, CA, USA.

Protein import into chloroplasts is carried out by the protein translocons at the outer and inner envelope membranes (TOC and TIC). Detailed structures for these translocons are lacking, with only a low-resolution TOC complex structure available. Recently, we showed that the TOC/TIC translocons can import folded proteins, a rather unique feat for a coupled double membrane system. Read More

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http://dx.doi.org/10.1002/1873-3468.13342DOI Listing
March 2019
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Convergent evolution of the Cys decarboxylases involved in aminovinyl-cysteine (AviCys) biosynthesis.

FEBS Lett 2019 Mar 7;593(6):573-580. Epub 2019 Mar 7.

Department of Chemistry, Fudan University, Shanghai, China.

S-[(Z)-2-aminovinyl]-d-cysteine (AviCys) is a unique motif found in several classes of ribosomally synthesized and post-translationally modified peptides (RiPPs). Biosynthesis of AviCys requires flavin-dependent Cys decarboxylases, which are highly divergent among different RiPP classes. In this study, we solved the crystal structure of the cypemycin decarboxylase CypD. Read More

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http://dx.doi.org/10.1002/1873-3468.13341DOI Listing
March 2019
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3.169 Impact Factor