48,150 results match your criteria FEBS letters[Journal]


Organophosphate hydrolase interacts with Ton components and is targeted to the membrane only in the presence of the ExbB/ExbD complex.

FEBS Lett 2019 Feb 21. Epub 2019 Feb 21.

Dept. of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad, 500046, India.

Our study aims to investigate the physiological role of organophosphate hydrolase (OPH), hitherto known for its involvement in the degradation of organophosphate insecticides and nerve agents in Sphingobium fuliginis. We find that OPH exists as part of the TonB-dependent Transport (TonBDT) system that is involved in nutrient transport across the bacterial outer membrane. OPH interacts physically with the Ton complex components ExbD and TonB. Read More

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http://dx.doi.org/10.1002/1873-3468.13345DOI Listing
February 2019

Transgenerational inheritance of cold temperature response in Drosophila.

FEBS Lett 2019 Feb 19. Epub 2019 Feb 19.

CSIR-Institute of Genomics and Integrative Biology, New Delhi.

Intergenerational inheritance of transcriptional responses induced by low temperature rearing has recently been shown in Drosophila. Besides germline inheritance, faecal transfer experiments indirectly suggested that the acquired microbiome may also have contributed to the transcriptional responses in offspring. Here, we analyze expression data on inheritance of the cold-induced effects in conjunction with previously reported transcriptomic differences between flies with a microbiota or axenic flies and provide support for a contribution of the acquired microbiome to the offspring phenotype. Read More

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http://dx.doi.org/10.1002/1873-3468.13343DOI Listing
February 2019

The peroxisome biogenesis factors Pex3 and Pex19: multitasking proteins with disputed functions.

FEBS Lett 2019 Feb 18. Epub 2019 Feb 18.

Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, the Netherlands.

The peroxisomal membrane protein Pex3 and its cytosolic interaction partner Pex19 have been implicated in peroxisomal membrane biogenesis. Although these peroxins have been extensively studied, no consensus has been reached yet on how they operate. Here, we discuss two major models of their function, namely, in direct insertion of proteins into the peroxisomal membrane or in formation of peroxisomal membrane protein-containing vesicles from the endoplasmic reticulum. Read More

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http://dx.doi.org/10.1002/1873-3468.13340DOI Listing
February 2019

Structural Considerations of Folded Protein Import through the Chloroplast TOC/TIC Translocons.

FEBS Lett 2019 Feb 18. Epub 2019 Feb 18.

Department of Plant Biology, University of California Davis, One Shields Avenue, Davis, CA, 95616.

Protein import into chloroplasts is carried out by the protein translocons at the outer and inner envelope membranes (TOC and TIC). Detailed structures for these translocons are lacking, with only a low-resolution TOC complex structure available. Recently, we showed that the TOC/TIC translocons can import folded proteins, a rather unique feat for a coupled double membrane system. Read More

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http://dx.doi.org/10.1002/1873-3468.13342DOI Listing
February 2019

Convergent evolution of the Cys decarboxylases involved in aminovinyl-cysteine (AviCys) biosynthesis.

FEBS Lett 2019 Feb 16. Epub 2019 Feb 16.

Department of Chemistry, Fudan University, Shanghai, 200433, China.

S-[(Z)-2-aminovinyl]-D-cysteine (AviCys) is a unique motif found in several classes of ribosomally synthesized and post-translationally modified peptides (RiPPs). Biosynthesis of AviCys requires flavin-dependent Cys decarboxylases, which are highly divergent among different RiPP classes. In this study, we solved the crystal structure of the cypemycin decarboxylase CypD. Read More

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http://dx.doi.org/10.1002/1873-3468.13341DOI Listing
February 2019

Genes of the undead: hibernation and death display different gene profiles.

FEBS Lett 2019 Feb 15. Epub 2019 Feb 15.

Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario, Canada, K1S 5B6.

A degree of regulation continues into death according to post-mortem transcriptome studies, which have identified "zombie genes" that come alive hours and days after organismal death. We hypothesized that hibernation, representing the closest natural mammalian phenomenon to death, would display similar gene expression profiles. Exploring zombie genes using qPCR and available transcriptomic resources from multiple torpid tissues in thirteen-lined ground squirrels show little in common with gene profiles observed following death. Read More

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http://dx.doi.org/10.1002/1873-3468.13338DOI Listing
February 2019
1 Read

Endocytosis-dependent lysosomal degradation of Src induced by protease-activated receptor 1.

FEBS Lett 2019 Feb 13. Epub 2019 Feb 13.

Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu, Taiwan, 30013, Republic of China.

Src plays a critical role in regulating cellular responses induced by protease-activated receptor 1 (PAR1). Here, we found that PAR1 activation induces lysosomal degradation of Src. Src is associated and trafficked together with activated PAR1 to early endosomes and then sorted to lysosomes for degradation. Read More

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http://dx.doi.org/10.1002/1873-3468.13336DOI Listing
February 2019

The histone demethylase PHF8 facilitates alternative splicing of the histocompatibility antigen HLA-G.

FEBS Lett 2019 Feb 13. Epub 2019 Feb 13.

Institute for Cardiovascular Physiology, Medical Faculty, Goethe University, Frankfurt, Germany.

Histone3-Lysine9 (H3K9) residues not only control gene expression, but also contribute to RNA splicing. Here, the H3K9 histone demethylase PHF8 was investigated in endothelial cells for its involvement in alternative splicing. An angiogenic sprouting assay shows the importance of PHF8 for endothelial cells. Read More

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http://dx.doi.org/10.1002/1873-3468.13337DOI Listing
February 2019
1 Read

Importance of micelle-like multimers in the atypical aggregation kinetics of N-terminal serum amyloid A peptides.

FEBS Lett 2019 Feb 13. Epub 2019 Feb 13.

Department of Chemistry and Biochemistry, California Polytechnic State University, San Luis Obispo, CA, 93407, United States.

Amyloid formation occurs via numerous complex mechanisms, often involving intermediates. This study examines the mechanism of amyloidogenesis in two N-terminal fragments of serum amyloid A (SAA), which are known to exhibit dramatically different amyloid structures. Fibrillization kinetics by these peptides are found to exhibit two unusual features: slower rates at higher peptide concentration, and complete insensitivity to addition of pre-formed seed. Read More

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http://dx.doi.org/10.1002/1873-3468.13334DOI Listing
February 2019

Secondary structure and topology of the transmembrane domain of Syndecan-2 in detergent micelles.

FEBS Lett 2019 Feb 11. Epub 2019 Feb 11.

Experimental Therapeutics Centre, Experimental Drug Development Centre (EDDC), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.

Syndecans are single-span membrane proteins playing important roles in cell-cell and cell-matrix interactions. The transmembrane domain of syndecans is critical for signal transduction across the cell membrane. Here, the structure of the transmembrane domain of syndecan-2 in detergent micelles was investigated using solution NMR spectroscopy. Read More

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http://dx.doi.org/10.1002/1873-3468.13335DOI Listing
February 2019

Methylene blue does not bypass Complex III antimycin block in mouse brain mitochondria.

FEBS Lett 2019 Feb 7. Epub 2019 Feb 7.

Brain and Mind Research Institute, Weill Medical College of Cornell University, New York, NY, USA.

Methylene blue (MB) is a promising prodrug to treat mitochondrial dysfunctions that is currently being used in clinical trials for Alzheimer's disease. MB can penetrate the blood brain barrier, accumulating in brain mitochondria where it acts as a redox mediator in the electron transfer chain (ETC). Mitochondrial flavins are thought to reduce MB, which is then oxidized by cytochrome c, thereby bypassing inhibited Complex I of ETC. Read More

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http://dx.doi.org/10.1002/1873-3468.13332DOI Listing
February 2019

Crystal structure of archaeal HMG-CoA reductase: insights into structural changes of the C-terminal helix of the class-I enzyme.

FEBS Lett 2019 Jan 31. Epub 2019 Jan 31.

Microbial Protein Structure Group, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.

3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) catalyses the last step in mevalonate biosynthesis. HMGR is the target of statin inhibitors that regulate cholesterol concentration in human blood. Here, we report the properties and structures of HMGR from an archaeon Methanothermococcus thermolithotrophicus (mHMGR). Read More

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http://doi.wiley.com/10.1002/1873-3468.13331
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http://dx.doi.org/10.1002/1873-3468.13331DOI Listing
January 2019
1 Read

Alternate quinone coupling in a new class of succinate dehydrogenase may potentiate mycobacterial respiratory control.

FEBS Lett 2019 Jan 24. Epub 2019 Jan 24.

Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.

There is a paucity of information on the unique components that pathogens use to form respiratory chains. It is not known why mycobacteria encode multiple succinate dehydrogenases (SDHs) to perform menaquinone-linked succinate oxidation, a thermodynamically unfavorable reaction (ΔG° = +21 kJ·mol ). In other bacteria, specific di-heme SDHs overcome this using the proton motive force. Read More

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http://dx.doi.org/10.1002/1873-3468.13330DOI Listing
January 2019

Crystal structure of the human Scribble PDZ1 domain bound to the PDZ-binding motif of APC.

FEBS Lett 2019 Jan 19. Epub 2019 Jan 19.

Department of Biochemistry & Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Australia.

Scribble (SCRIB) is an important adaptor protein that controls the establishment and maintenance of apico-basal cell polarity. To better understand how SCRIB controls cell polarity signalling via its PDZ domains, we investigated human SCRIB interactions with adenomatous polyposis coli (APC). We show that SCRIB PDZ1, PDZ2 and PDZ3 are the major interactors with the APC PDZ-binding motif (PBM), whereas SCRIB PDZ4 does not show detectable binding to APC. Read More

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http://dx.doi.org/10.1002/1873-3468.13329DOI Listing
January 2019
2 Reads

Glucocorticoid receptor suppresses gene expression of Rev-erbα (Nr1d1) through interaction with the CLOCK complex.

FEBS Lett 2019 Jan 19. Epub 2019 Jan 19.

Department of Internal Medicine (Endocrinology and Metabolism), Faculty of Medicine, University of Tsukuba, Japan.

Glucocorticoids have various medical uses but are accompanied by side effects. The glucocorticoid receptor (GR) has been reported to regulate the clock genes, but the underlying mechanisms are incompletely understood. In this study, we focused on the suppressive effect of the GR on the expression of Rev-erbα (Nr1d1), an important component of the clock regulatory circuits. Read More

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http://dx.doi.org/10.1002/1873-3468.13328DOI Listing
January 2019
2 Reads

The transcription and expression profile of p53 mutant reveals new aspects of gain of function for mutant p53.

Authors:

FEBS Lett 2019 Jan 3;593(1):138. Epub 2019 Jan 3.

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http://dx.doi.org/10.1002/1873-3468.13322DOI Listing
January 2019
1 Read

SWAP70 undergoes dynamic conformational regulation at the leading edge of migrating cells.

FEBS Lett 2019 Jan 12. Epub 2019 Jan 12.

Institute of Biological Chemistry, Biophysics and Bioengineering, School of Engineering and Physical Sciences, Heriot Watt University, Edinburgh, UK.

Rearrangements of the actin cytoskeleton are regulated in part by dynamic localised activation and inactivation of Rho family small GTPases. SWAP70 binds to and activates the small GTPase RAC1 as well as binding to filamentous actin and PIP . We have developed an encoded biosensor, which uses Forster resonance energy transfer to reveal conformational changes in SWAP70 in live cells. Read More

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http://doi.wiley.com/10.1002/1873-3468.13326
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http://dx.doi.org/10.1002/1873-3468.13326DOI Listing
January 2019
6 Reads

Acetylation modulates LC3 stability and cargo recognition.

FEBS Lett 2019 Jan 11. Epub 2019 Jan 11.

Medical School, Chongqing University, China.

LC3 is a key autophagy-related protein involved in both autophagosome formation and autophagy cargo recruitment. Despite these functions being exerted by deacetylated LC3, this protein is more abundantly distributed in its acetylated form. Here, we reveal that the stability and cargo recognition ability of LC3 are highly dependent on its acetylation. Read More

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http://doi.wiley.com/10.1002/1873-3468.13327
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http://dx.doi.org/10.1002/1873-3468.13327DOI Listing
January 2019
10 Reads

Generation of mesothelial progenitor-like cells from mouse-induced pluripotent stem cells.

FEBS Lett 2019 Jan 4. Epub 2019 Jan 4.

Institute for Quantitative Biosciences, The University of Tokyo, Japan.

Mesothelial cells, which cover the surface of visceral organs and serous cavities in mammals, play a crucial role in preventing adhesion. We previously reported that primary mesothelial progenitor cells (MPCs) can not only prevent postoperative adhesion but also promote liver regeneration after hepatectomy. Induced pluripotent stem cells (iPSCs) have the potential to be used for regenerative medicine. Read More

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http://dx.doi.org/10.1002/1873-3468.13325DOI Listing
January 2019
1 Read

Giardia lamblia Hsp90 pre-mRNAs undergo self-splicing to generate mature RNA in an in vitro trans-splicing reaction.

FEBS Lett 2019 Jan 3. Epub 2019 Jan 3.

Department of Biochemistry, Indian Institute of Science, Bangalore, India.

We have previously shown that the Heat Shock Protein 90 (Hsp90) gene in G. lamblia is expressed from two ORFs localized 777 kb apart. The pre-mRNAs transcribed from these ORFs are stitched by a trans-splicing mechanism. Read More

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http://doi.wiley.com/10.1002/1873-3468.13324
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http://dx.doi.org/10.1002/1873-3468.13324DOI Listing
January 2019
8 Reads

USP10 regulates Musashi-2 stability via deubiquitination and promotes tumour proliferation in colon cancer.

FEBS Lett 2019 Jan 3. Epub 2019 Jan 3.

Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, China.

Recent studies have demonstrated that ubiquitin-specific protease 10 (USP10) plays a catalytic role in tumour suppression mainly by deubiquitinating its target proteins to enhance their stabilities. However, we found that USP10 could interact with and regulate the expression of oncogenic factor Musashi-2 (MSI2). We investigated whether USP10 positively regulates the expression of MSI2 by deubiquitination and confirmed the type of polyubiquitin chain that is linked to MSI2. Read More

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http://doi.wiley.com/10.1002/1873-3468.13323
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http://dx.doi.org/10.1002/1873-3468.13323DOI Listing
January 2019
13 Reads

Interaction of merozoite surface protein 2 with lipid membranes.

FEBS Lett 2019 Feb 10;593(3):288-295. Epub 2019 Jan 10.

School of Life Sciences, Anhui University, Hefei, China.

Merozoite surface protein 2 (MSP2) is a potential vaccine candidate against malaria, although its functional role is yet to be elucidated. Previous studies showed that MSP2 can interact with membranes, which may facilitate merozoite invasion into the host cell. The N-terminal 25 residues of MSP2 (MSP2 ), which may be aggregated on the merozoite surface, play a key role in the interaction with membranes. Read More

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http://dx.doi.org/10.1002/1873-3468.13320DOI Listing
February 2019
3 Reads
3.169 Impact Factor

Crystal structures and biochemical analyses of intermediate cleavage peptidase: role of dynamics in enzymatic function.

FEBS Lett 2018 Dec 24. Epub 2018 Dec 24.

High Pressure and Synchrotron Radiation Physics Division, Bhabha Atomic Research Centre, Mumbai, India.

Intermediate cleavage peptidase (Icp55) processes a subset of mitochondrial matrix proteins by removing a bulky residue at their N termini, leaving behind smaller N-terminal residues (icp activity). This contributes towards the stability of the mitochondrial proteome. We report crystal structures of yeast Icp55 including one bound to the apstatin inhibitor. Read More

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http://doi.wiley.com/10.1002/1873-3468.13321
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http://dx.doi.org/10.1002/1873-3468.13321DOI Listing
December 2018
8 Reads
3.169 Impact Factor

Mg -free ATP regulates the processivity of native cytoplasmic dynein.

FEBS Lett 2019 Feb 3;593(3):296-307. Epub 2019 Jan 3.

Molecular and Cell Physiology, Hannover Medical School, Germany.

Cytoplasmic dynein, a microtubule-based motor protein, is responsible for many cellular functions ranging from cargo transport to cell division. The various functions are carried out by a single isoform of cytoplasmic dynein, thus requiring different forms of motor regulation. A possible pathway to regulate motor function was revealed in optical trap experiments. Read More

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http://doi.wiley.com/10.1002/1873-3468.13319
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http://dx.doi.org/10.1002/1873-3468.13319DOI Listing
February 2019
7 Reads

YAPping about and not forgetting TAZ.

FEBS Lett 2019 Feb 15;593(3):253-276. Epub 2019 Jan 15.

Centre for Comparative Genomics, Murdoch University, Australia.

The Hippo pathway has emerged as a major eukaryotic signalling pathway and is increasingly the subject of intense interest, as are the key effectors of canonical Hippo signalling, YES-associated protein (YAP) and TAZ. The Hippo pathway has key roles in diverse biological processes, including network signalling regulation, development, organ growth, tissue repair and regeneration, cancer, stem cell regulation and mechanotransduction. YAP and TAZ are multidomain proteins and function as transcriptional coactivators of key genes to evoke their biological effects. Read More

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http://dx.doi.org/10.1002/1873-3468.13318DOI Listing
February 2019
2 Reads

Synaptotagmin oligomers are necessary and can be sufficient to form a Ca -sensitive fusion clamp.

FEBS Lett 2019 Jan 18;593(2):154-162. Epub 2019 Jan 18.

Department of Cell Biology, Yale University School of Medicine, New Haven, CT, USA.

The buttressed-ring hypothesis, supported by recent cryo-electron tomography analysis of docked synaptic-like vesicles in neuroendocrine cells, postulates that prefusion SNAREpins are stabilized and organized by Synaptotagmin (Syt) ring-like oligomers. Here, we use a reconstituted single-vesicle fusion analysis to test the prediction that destabilizing the Syt1 oligomers destabilizes the clamp and results in spontaneous fusion in the absence of Ca . Vesicles in which Syt oligomerization is compromised by a ring-destabilizing mutation dock and diffuse freely on the bilayer until they fuse spontaneously, similar to vesicles containing only v-SNAREs. Read More

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http://dx.doi.org/10.1002/1873-3468.13317DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6349546PMC
January 2019
2 Reads

Dual conformation of the ligand induces the partial agonistic activity of retinoid X receptor α (RXRα).

FEBS Lett 2019 Jan 21;593(2):242-250. Epub 2018 Dec 21.

Department of Chemistry, Rikkyo University, Tokyo, Japan.

1-[(3,5,5,8,8-pentamethyl-5,6,7,8-tetrahydronaphthalen-2-yl)amino]benzotriazole-5-carboxylic acid (CBt-PMN), a partial agonist of retinoid X receptor (RXR), has attracted attention due to its potential to treat type 2 diabetes and central nervous system diseases with reduced adverse effects of existing full agonists. Herein, we report the crystal structure of CBt-PMN-bound ligand-binding domain of human RXRα (hRXRα) and its biochemical characterization. Interestingly, the structure is a tetramer in nature, in which CBt-PMNs are clearly found binding in two different conformations. Read More

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http://dx.doi.org/10.1002/1873-3468.13301DOI Listing
January 2019
2 Reads

Symmetrical organization of proteins under docked synaptic vesicles.

FEBS Lett 2019 Jan 18;593(2):144-153. Epub 2019 Jan 18.

Department of Cell Biology, Yale University School of Medicine, New Haven, CT, USA.

During calcium-regulated exocytosis, the constitutive fusion machinery is 'clamped' in a partially assembled state until synchronously released by calcium. The protein machinery involved in this process is known, but the supra-molecular architecture and underlying mechanisms are unclear. Here, we use cryo-electron tomography analysis in nerve growth factor-differentiated neuro-endocrine (PC12) cells to delineate the organization of the release machinery under the docked vesicles. Read More

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http://doi.wiley.com/10.1002/1873-3468.13316
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http://dx.doi.org/10.1002/1873-3468.13316DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353562PMC
January 2019
9 Reads
3.169 Impact Factor

TRB1 negatively regulates gluconeogenesis by suppressing the transcriptional activity of FOXO1.

FEBS Lett 2019 Feb 8;593(3):369-380. Epub 2019 Jan 8.

Department of Cell Signaling, Graduate School of Pharmaceutical Sciences, Nagoya City University, Japan.

Tribbles related homolog 1 is the mammalian ortholog of Tribbles, which controls cell division and migration during development in Drosophila. TRB1 is a pseudokinase and functions as a scaffold protein. Recent findings suggest that TRB1 plays important roles in hepatic lipid metabolism and participates in insulin resistance. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/1873-3468.13
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http://dx.doi.org/10.1002/1873-3468.13314DOI Listing
February 2019
7 Reads

Modulation of ABA responses by the protein kinase WNK8.

FEBS Lett 2019 Feb 28;593(3):339-351. Epub 2018 Dec 28.

Department of Cell Biology, Centre for Organismal Studies, Ruprecht-Karls-Universität Heidelberg, Germany.

Abscisic acid (ABA) regulates growth and developmental processes in response to limiting water conditions. ABA functions through a core signaling pathway consisting of PYR1/PYL/RCAR ABA receptors, type 2C protein phosphatases (PP2Cs), and SnRK2-type protein kinases. Other signaling modules might converge with ABA signals through the modulation of core ABA signaling components. Read More

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http://dx.doi.org/10.1002/1873-3468.13315DOI Listing
February 2019
4 Reads
3.169 Impact Factor

Writing a great abstract: tips from an Editor.

Authors:
Daniela Ruffell

FEBS Lett 2019 Jan 15;593(2):141-143. Epub 2018 Dec 15.

FEBS Letters Editorial Office, Heidelberg University Biochemistry Centre, Germany.

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http://dx.doi.org/10.1002/1873-3468.13304DOI Listing
January 2019
1 Read

Jim's view: analog to digital conversion in biology.

Authors:
James E Rothman

FEBS Lett 2018 Dec 15;592(24):4009-4010. Epub 2018 Dec 15.

Department of Cell Biology, Yale University School of Medicine, New Haven, CT, USA.

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http://dx.doi.org/10.1002/1873-3468.13308DOI Listing
December 2018
1 Read

New horizons in Glycobiology research.

Authors:
Sandro Sonnino

FEBS Lett 2018 Dec;592(23):3771-3772

Department of Medical Biotechnology and Translational Medicine, University of Milan, Italy.

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http://dx.doi.org/10.1002/1873-3468.13300DOI Listing
December 2018
1 Read

Dual role of the σ factor in primer RNA synthesis by bacterial RNA polymerase.

FEBS Lett 2019 Feb 20;593(3):361-368. Epub 2018 Dec 20.

Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia.

Bacterial RNA polymerase (RNAP) serves as a primase during replication of single-stranded plasmids and filamentous phages. Primer RNA (prRNA) synthesis from the origin regions of these replicons depends on the σ factor that normally participates in promoter recognition. However, it was proposed that σ may not be required for origin recognition but is rather involved in RNA extension by RNAP. Read More

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http://dx.doi.org/10.1002/1873-3468.13312DOI Listing
February 2019
6 Reads

Time-resolved interaction proteomics of the GIGANTEA protein under diurnal cycles in Arabidopsis.

FEBS Lett 2019 Feb 28;593(3):319-338. Epub 2018 Dec 28.

SynthSys and School of Biological Sciences, University of Edinburgh, UK.

The plant-specific protein GIGANTEA (GI) controls many developmental and physiological processes, mediating rhythmic post-translational regulation. GI physically binds several proteins implicated in the circadian clock, photoperiodic flowering, and abiotic stress responses. To understand GI's multifaceted function, we aimed to comprehensively and quantitatively identify potential interactors of GI in a time-specific manner, using proteomics on Arabidopsis plants expressing epitope-tagged GI. Read More

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http://dx.doi.org/10.1002/1873-3468.13311DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6373471PMC
February 2019
3 Reads

Identification of temperature-sensitive mutations and characterization of thermolabile RNase II variants.

FEBS Lett 2019 Feb 28;593(3):352-360. Epub 2018 Dec 28.

Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.

The RNase II family of ribonucleases is ubiquitous and critical for RNA metabolism. The rnb500 allele has been widely used for over 30 years; however, the underlying genetic changes which result in RNase II thermolabile activity remain unknown. Here, we combine molecular and biophysical studies to carry out an in vivo and in vitro investigation of RNase II mutation(s) that confer the rnb500 phenotype. Read More

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http://dx.doi.org/10.1002/1873-3468.13313DOI Listing
February 2019
1 Read

TDP-43 accelerates deadenylation of target mRNAs by recruiting Caf1 deadenylase.

FEBS Lett 2019 Feb 25;593(3):277-287. Epub 2019 Jan 25.

Department of Biological Chemistry, Graduate School of Pharmaceutical Sciences, Nagoya City University, Japan.

TAR DNA-binding protein 43 (TDP-43) is an RNA-binding protein, whose loss-of-function mutation causes amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration. Recent studies demonstrated that TDP-43 binds to the 3' untranslated region (UTR) of target mRNAs to promote mRNA instability. Here, we show that TDP-43 recruits Caf1 deadenylase to mRNA targets and accelerates their deadenylation. Read More

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http://dx.doi.org/10.1002/1873-3468.13310DOI Listing
February 2019
2 Reads

Stereoselective catalysis controlled by a native leucine or variant isoleucine wing-gatekeeper in 2-haloacid dehalogenase.

FEBS Lett 2019 Feb 28;593(3):308-318. Epub 2018 Dec 28.

Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.

Comprehensively understanding enzymatic stereoselectivity will assist in the creation of new enzymes for producing optically pure compounds for chemical applications. The essential features for selecting enantiomers are controlled by particular residues or regions of the enzymes. We report a stereoselective mechanism in the D-2-haloacid dehalogenase HadD AJ1, in which L288 is identified as a gatekeeper in the access channel that strictly recognizes D-enantiomers. Read More

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http://dx.doi.org/10.1002/1873-3468.13309DOI Listing
February 2019
11 Reads

The flavonoid baicalin improves glucose metabolism by targeting the PH domain of AKT and activating AKT/GSK3β phosphorylation.

FEBS Lett 2019 Jan 17;593(2):175-186. Epub 2018 Dec 17.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, China.

Baicalin is one of the main flavonoids of the dried root of Scutellaria baicalensis Georgi and is reported to exert beneficial effects on the regulation of glucose/lipid metabolism. However, understanding its specific target and unique mechanism for improving glucose utilization is a challenge. In this paper, target fishing with a baicalin probe reveals that baicalin interacts with AKT. Read More

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http://doi.wiley.com/10.1002/1873-3468.13305
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http://dx.doi.org/10.1002/1873-3468.13305DOI Listing
January 2019
15 Reads
3.169 Impact Factor

Novel functions of peroxiredoxin Q from Deinococcus radiodurans R1 as a peroxidase and a molecular chaperone.

FEBS Lett 2019 Jan 11;593(2):219-229. Epub 2018 Dec 11.

Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Korea.

Deinococcus radiodurans R1 is extremely resistant to ionizing radiation and oxidative stress. In this study, we characterized DR0846, a candidate peroxiredoxin in D. radiodurans. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/1873-3468.13
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http://dx.doi.org/10.1002/1873-3468.13302DOI Listing
January 2019
2 Reads
3.169 Impact Factor

Rab5 activation on macropinosomes requires ALS2, and subsequent Rab5 inactivation through ALS2 detachment requires active Rab7.

FEBS Lett 2019 Jan 7;593(2):230-241. Epub 2018 Dec 7.

Division of Biosignaling, Research Institute for Biomedical Sciences, Tokyo University of Science, Noda, Japan.

Macropinocytosis is a nonspecific bulk uptake of extracellular fluid. During endosome maturation, the Rab5-to-Rab7 switch machinery executes the conversion from early to late endosomes. However, how the Rab switch works during macropinosome maturation remains unclear. Read More

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http://dx.doi.org/10.1002/1873-3468.13306DOI Listing
January 2019
4 Reads

Environmental pH and a Glu364 to Gln mutation in the chlorophyll-binding CP47 protein affect redox-active TyrD and charge recombination in Photosystem II.

FEBS Lett 2019 Jan 18;593(2):163-174. Epub 2018 Dec 18.

Department of Biochemistry, University of Otago, Dunedin, New Zealand.

In Photosystem II, loop E of the chlorophyll-binding CP47 protein is located near a redox-active tyrosine, Y , forming a symmetrical analog to loop E in CP43, which provides a ligand to the oxygen-evolving complex (OEC). A Glu364 to Gln substitution in CP47, near Y , does not affect growth in the cyanobacterium Synechocystis sp. PCC 6803; however, deletion of the extrinsic protein PsbV in this mutant leads to a strain displaying a pH-sensitive phenotype. Read More

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http://doi.wiley.com/10.1002/1873-3468.13307
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http://dx.doi.org/10.1002/1873-3468.13307DOI Listing
January 2019
2 Reads

Arabidopsis thaliana α1,2-l-fucosyltransferase catalyzes the transfer of l-galactose to xyloglucan oligosaccharides.

FEBS Lett 2019 Jan 11;593(2):187-194. Epub 2018 Dec 11.

International Center for Biotechnology, Osaka University, Suita, Japan.

l-Galactose (l-Gal) is one of the components of plant cell wall polysaccharides. In the GDP-l-fucose-deficient Arabidopsis thaliana mutant mur1, l-fucose (l-Fuc) residues in xyloglucan are substituted by l-Gal residues. l-Gal only differs from l-Fuc by the presence of an oxygen at C-6. Read More

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http://dx.doi.org/10.1002/1873-3468.13303DOI Listing
January 2019
1 Read

Polyamines interfere with protein ubiquitylation and cause depletion of intracellular amino acids: a possible mechanism for cell growth inhibition.

FEBS Lett 2019 Jan 7;593(2):209-218. Epub 2018 Dec 7.

Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-C.S.I.C., Spain.

Spermidine is a polyamine present in eukaryotes with essential functions in protein synthesis. At high concentrations spermidine and norspermidine inhibit growth by unknown mechanisms. Transcriptomic analysis of the effect of norspermidine on the plant Arabidopsis thaliana indicates upregulation of the response to heat stress and denatured proteins. Read More

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http://doi.wiley.com/10.1002/1873-3468.13299
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http://dx.doi.org/10.1002/1873-3468.13299DOI Listing
January 2019
14 Reads

GLUT6 is a lysosomal transporter that is regulated by inflammatory stimuli and modulates glycolysis in macrophages.

FEBS Lett 2019 Jan 4;593(2):195-208. Epub 2018 Dec 4.

Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.

The solute carrier family is an important protein class governing compound transport across membranes. However, some of its members remain functionally unidentified. We analyzed ChIP-seq data for the NF-κB family transcription factor RelA and identified GLUT6 as a functionally uncharacterized transporter that putatively works in inflammatory responses. Read More

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http://dx.doi.org/10.1002/1873-3468.13298DOI Listing
January 2019
2 Reads

Suppression of superoxide production by a spin-spin coupling between semiquinone and the Rieske cluster in cytochrome bc.

FEBS Lett 2019 Jan 3;593(1):3-12. Epub 2018 Dec 3.

Department of Molecular Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.

Catalytic reactions of quinol oxidoreductases may lead to the generation of superoxide due to electron leaks from unstable semiquinone intermediates (SQ). For cytochrome bc , the mechanism of suppression of superoxide generation remains unknown. We analyzed conditions of formation of a spin-spin-coupled state between SQ and the Rieske cluster (SQ-FeS) associated with catalysis of the quinol oxidation site of cytochrome bc . Read More

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http://doi.wiley.com/10.1002/1873-3468.13296
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http://dx.doi.org/10.1002/1873-3468.13296DOI Listing
January 2019
11 Reads

Bacterial CYP154C8 catalyzes carbon-carbon bond cleavage in steroids.

FEBS Lett 2019 Jan 8;593(1):67-79. Epub 2018 Dec 8.

Department of Life Science and Biochemical Engineering, SunMoon University, Asan-si, Korea.

Here, we report the first bacterial cytochrome P450, CYP154C8, that catalyzes the C-C bond cleavage reaction of steroids. A major change in product distribution is observed with CYP154C8, when the reactions are supported by NADPH and spinach redox partners ferredoxin and ferredoxin reductase, compared with previously reported reactions supported by NADH and redox partners containing putidaredoxin and putidaredoxin reductase. The NMR-based structural elucidation of reaction products reveals 21-hydroxyprednisone as the major product for prednisone, while the other product is identified as 1-dehydroadrenosterone obtained due to C-C bond cleavage. Read More

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http://doi.wiley.com/10.1002/1873-3468.13297
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http://dx.doi.org/10.1002/1873-3468.13297DOI Listing
January 2019
8 Reads

The EU Court of Justice extends the GMO Directive to gene-edited organisms.

Authors:
Daniela Ruffell

FEBS Lett 2018 Nov;592(22):3653-3657

FEBS Letters Editorial Office, Heidelberg University Biochemistry Centre, Germany.

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http://dx.doi.org/10.1002/1873-3468.13293DOI Listing
November 2018
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The PI 3-kinase PI3KC2α regulates mouse platelet membrane structure and function independently of membrane lipid composition.

FEBS Lett 2019 Jan 24;593(1):88-96. Epub 2018 Nov 24.

Australian Centre for Blood Diseases, Monash University, Melbourne, Australia.

PI3KC2α is a phosphoinositide 3-kinase with a recently reported function in platelets; PI3KC2α-deficient mouse platelets have altered membrane structure and impaired function. Yet, how these membrane changes cause platelet dysfunction remains unknown. Here, focused ion beam-scanning electron microscopy of PI3KC2α-deficient platelet ultrastructure reveals a specific effect on the internal membrane structure, while liquid chromatography-tandem mass spectrometry profiling of 294 lipid species shows unaltered lipid composition. Read More

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http://doi.wiley.com/10.1002/1873-3468.13295
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http://dx.doi.org/10.1002/1873-3468.13295DOI Listing
January 2019
8 Reads

FEBS Letters Special Issue on Exocytosis and Endocytosis.

FEBS Lett 2018 Nov;592(21):3477-3479

Nanobiology Institute, Yale University, West Haven, CT, USA.

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http://dx.doi.org/10.1002/1873-3468.13274DOI Listing
November 2018
2 Reads