6,333 results match your criteria FEBS Journal[Journal]


Turning a potent family-9 free cellulase into an operational cellulosomal component and vice versa.

FEBS J 2019 Apr 20. Epub 2019 Apr 20.

Aix Marseille Univ, CNRS, LCB, Marseille, France.

Ruminiclostridium cellulolyticum and Lachnoclostridium phytofermentans are cellulolytic clostridia either producing extracellular multienzymatic complexes termed cellulosomes or secreting free cellulases, respectively. In the free state, the cellulase Cel9A secreted by L. phytofermentans is much more active on crystalline cellulose than any cellulosomal family-9 enzyme produced by R. Read More

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http://dx.doi.org/10.1111/febs.14858DOI Listing

Colorectal cancer-associated ~6 kDa hyaluronan serves as a novel biomarker for cancer progression and metastasis.

FEBS J 2019 Apr 19. Epub 2019 Apr 19.

Department of Molecular Biology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Low molecular weight hyaluronan (LMW-HA) is believed to accumulate in tumors and to exert pro-tumor effects. This study aimed to identify colorectal cancer (CRC)-associated LMW-HA, precisely determine its molecular weight and elucidate its role in predicting tumor progression. The molecular weight distribution of hyaluronan extracted from CRC and paired non-cancerous tissues was evaluated. Read More

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http://dx.doi.org/10.1111/febs.14859DOI Listing

Zelda and the maternal-to-zygotic transition in cockroaches.

FEBS J 2019 Apr 16. Epub 2019 Apr 16.

Institute of Evolutionary Biology CSIC-Universitat Pompeu Fabra, Passeig Marítim 37, 08003, Barcelona, Spain.

In the endopterygote Drosophila melanogaster, Zelda is an activator of the zygotic genome during the maternal-to-zygotic transition (MZT). Zelda binds cis-regulatory elements (TAGteam heptamers), making chromatin accessible for gene transcription. Zelda has been studied in other endopterygotes: Apis mellifera and Tribolium castaneum, and the paraneopteran Rhodnius prolixus. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/febs.14856
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http://dx.doi.org/10.1111/febs.14856DOI Listing
April 2019
1 Read

Deciphering the number and location of active sites in the monomeric glyoxalase I of Zea mays.

FEBS J 2019 Apr 16. Epub 2019 Apr 16.

Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Universidad Nacional de Rosario, Suipacha 531, S2002LRK, Rosario, Argentina.

Detoxification of methylglyoxal, a toxic by-product of central sugar metabolism, is a major issue for all forms of life. The glyoxalase pathway evolved to effectively convert methylglyoxal into D-lactate via a glutathione hemithioacetal intermediate. Recently, we have shown that the monomeric glyoxalase I from maize exhibits a symmetric fold with two cavities, potentially harboring two active sites, in analogy with homodimeric enzyme surrogates. Read More

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http://dx.doi.org/10.1111/febs.14855DOI Listing
April 2019
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Metabolomics reveals tepotinib-related mitochondrial dysfunction in MET activating mutations-driven models.

FEBS J 2019 Apr 16. Epub 2019 Apr 16.

Department of Radiation Oncology, Inselspital, Bern University Hospital, University of Bern, 3010, Bern, Switzerland.

Genetic aberrations in the hepatocyte growth factor receptor tyrosine kinase MET induce oncogenic addiction in various types of human cancers, advocating MET as a viable anticancer target. Here, we report that MET signaling plays an important role in conferring a unique metabolic phenotype to cellular models expressing MET-activating mutated variants that are either sensitive or resistant towards MET small molecule inhibitors. MET phosphorylation downregulated by the specific MET inhibitor tepotinib resulted in markedly decreased viability and increased apoptosis in tepotinib-sensitive cells. Read More

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http://dx.doi.org/10.1111/febs.14852DOI Listing

A telomerase subunit homolog La protein from Trypanosoma brucei plays an essential role in ribosomal biogenesis.

FEBS J 2019 Apr 16. Epub 2019 Apr 16.

Hefei National Laboratory for Physical Science at Microscale and School of Life Science, University of Science and Technology of China, Hefei, Anhui, People's Republic of China.

The autoantigen La protein is an important component of telomerase and a predominantly nuclear phosphoprotein. As a telomerase subunit, La protein associates with the telomerase ribonucleoprotein and influences telomere length. In the reverse transcription, La protein stimulates enzymatic activity and increases repeated addition processivity of telomerase. Read More

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http://dx.doi.org/10.1111/febs.14853DOI Listing

Two Nimrod receptors, NimC1 and Eater, synergistically contribute to bacterial phagocytosis in Drosophila melanogaster.

FEBS J 2019 Apr 16. Epub 2019 Apr 16.

Global Health Institute, School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.

Eater and NimC1 are transmembrane receptors of the Drosophila Nimrod family, specifically expressed in hemocytes, the insect blood cells. Previous ex vivo and in vivo RNAi studies have pointed to their role in the phagocytosis of bacteria. Here, we have created a novel NimC1 null mutant to re-evaluate the role of NimC1, alone or in combination with Eater, in the cellular immune response. Read More

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http://dx.doi.org/10.1111/febs.14857DOI Listing

Posttranslational modifications of titin from cardiac muscle: how, where and what for?

FEBS J 2019 Apr 15. Epub 2019 Apr 15.

Institute of Physiology II, University of Muenster, Robert-Koch-Str. 27b, D-48149, Muenster, Germany.

Titin is a giant elastic protein expressed in the contractile units of striated muscle cells, including the sarcomeres of cardiomyocytes. The last decade has seen enormous progress in our understanding of how titin molecular elasticity is modulated in a dynamic manner to help cardiac sarcomeres adjust to the varying hemodynamic demands on the heart. Crucial events mediating the rapid modulation of cardiac titin stiffness are posttranslational modifications (PTMs) of titin. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/febs.14854
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http://dx.doi.org/10.1111/febs.14854DOI Listing
April 2019
1 Read

Cooperation between β-galactosidase and an isoprimeverose-producing oligoxyloglucan hydrolase is key for xyloglucan degradation in Aspergillus oryzae.

FEBS J 2019 Apr 13. Epub 2019 Apr 13.

Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan.

The galactosylation of xyloglucan blocks many of the enzymatic processes targeting this oligosaccharide. We found that the expression of a gene encoding Aspergillus oryzae β-galactosidase (LacA) is induced in the presence of xyloglucan oligosaccharides. With detailed analyses of the substrate specificity of purified recombinant LacA, we show that LacA cleaves galactopyranosyl residues from xyloglucan oligosaccharides, but not from xyloglucan polysaccharide, and plays a vital role in xyloglucan degradation. Read More

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http://dx.doi.org/10.1111/febs.14848DOI Listing

Structural analyses reveal that MBD3 is a methylated-CG binder.

FEBS J 2019 Apr 13. Epub 2019 Apr 13.

Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, PR China.

MBD3, a methyl-CpG binding domain (MBD)-containing protein, is a core subunit of the Mi-2/NuRD complex. Recent reports show that MBD3 recognizes both methylated CG (mCG)- and hydroxymethylated CG (hmCG)-containing DNA, with a preference for hmCG. However, whether the MBD3-MBD indeed has methyl-CG binding ability is controversial. Read More

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http://dx.doi.org/10.1111/febs.14850DOI Listing

Beyond amino acid sequence: disulfide bonds and the origins of the extreme amyloidogenic properties of insulin's H-fragment.

FEBS J 2019 Apr 13. Epub 2019 Apr 13.

Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, 1 Pasteur Str, 02-093, Warsaw, Poland.

The presence of disulfide bonds affects the protein stability and therefore tendency to misfold and form amyloid-like fibrils. Insulin's three disulfide bridges stabilize the native state and prevent aggregation. Partial proteolysis of insulin releases highly amyloidogenic and inherently disordered two-chain 'H-fragment' retaining insulin's Cys7A-Cys7B and Cys6A-Cys11A disulfide bonds. Read More

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http://dx.doi.org/10.1111/febs.14849DOI Listing

Dissecting the role of hyaluronan synthases in the tumor microenvironment.

FEBS J 2019 Apr 11. Epub 2019 Apr 11.

Department of Pathology, Anatomy and Cell Biology and the Cancer Cell Biology and Signaling Program, Sidney Kimmel Cancer Center, Sidney Kimmel Medical College at Thomas Jefferson University, Philadelphia, PA, USA.

The tumor microenvironment is becoming a crucial factor in determining the aggressiveness of neoplastic cells. The glycosaminoglycan hyaluronan is one of the principal constituents of both the tumor stroma and the cancer cell surfaces, and its accumulation can dramatically influence patient survival. Hyaluronan functions are dictated by its ability to interact with several signaling receptors that often activate pro-angiogenic and pro-tumorigenic intracellular pathways. Read More

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http://dx.doi.org/10.1111/febs.14847DOI Listing

Characterizing lysine acetylation of Escherichia coli type II citrate synthase.

FEBS J 2019 Apr 11. Epub 2019 Apr 11.

Cell and Molecular Biology Program, University of Arkansas, Fayetteville, AR, USA.

The citrate synthase (CS) catalyzes the first reaction of the tricarboxylic acid cycle, playing an important role in central metabolism. The acetylation of lysine residues in the Escherichia coli Type II CS has been identified at multiple sites by proteomic studies, but their effects remain unknown. In this study, we applied the genetic code expansion strategy to generate 10 site-specifically acetylated CS variants which have been identified in nature. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/febs.14845
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http://dx.doi.org/10.1111/febs.14845DOI Listing
April 2019
1 Read

Structural Basis for the Target DNA Recognition and Binding by the MYB Domain of Phosphate Starvation Response 1.

FEBS J 2019 Apr 11. Epub 2019 Apr 11.

Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, College of Life Science, Fujian Normal University, Fuzhou350117, China.

The phosphate starvation response 1 (PHR1) protein has a central role in mediating the response to phosphate starvation in plants. PHR1 is composed of a number of domains including a MYB domain involved with DNA-binding and a coiled-coil domain proposed to be involved with dimer formation. PHR1 binds to the promoter of phosphate starvation-induced genes to control the levels of phosphate required for nutrition. Read More

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http://dx.doi.org/10.1111/febs.14846DOI Listing

Structural insight into the unique dsDNA binding topology of the human ORC2 wing helix domain.

FEBS J 2019 Apr 8. Epub 2019 Apr 8.

Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, University of Science and Technology of China, Hefei, China.

The origin recognition complex (ORC) is indispensable for the initiation of DNA replication during the cell cycle. The DNA-binding modes of the human ORC winged helix domain (WHD) remain enigmatic, as the dsDNA recognition sites of archaeal and Saccharomyces cerevisiae ORC WHDs are distinct. Here, we solved the high-resolution crystal structure of the human ORC2 WHD, although its complex with dsDNA is difficult to crystallize due to its weak binding affinities. Read More

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http://dx.doi.org/10.1111/febs.14844DOI Listing

Inflammation associated with chronic heart failure leads to enhanced susceptibility to depression.

FEBS J 2019 Apr 8. Epub 2019 Apr 8.

Department of Translational Neuroscience, Jing' an District Centre Hospital of Shanghai, State Key Laboratory of Medical Neurobiology & Institutes of Brain Science, Fudan University, China.

Epidemiological and clinicopathological studies indicate that there is a high risk for chronic heart failure (CHF) in patients suffering from neuropsychiatric disorders, such as depression. However, it is unclear whether CHF causes depression, and the underlying mechanisms of this association remain largely unknown. In this study, mice with myocardial infarction and CHF were used to investigate behavioral alterations as well as changes in the brain-heart axis. Read More

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http://dx.doi.org/10.1111/febs.14839DOI Listing

Age-induced oxidative stress impairs adipogenesis and thermogenesis in brown fat.

FEBS J 2019 Apr 8. Epub 2019 Apr 8.

Nanjing Maternal and Child Health Medical Institute, Nanjing Maternity and Child Health Care Hospital, Women's Hospital of Nanjing Medical University, China.

It is well-established that the mass and function of human brown adipose tissue (BAT) declines with age. A key factor involved in age-related impairment of BAT is oxidative stress; however, there is a paucity of studies to date that have explored this relationship. Here, we characterized the age-related molecular and functional alterations in BAT in vivo in mice of different ages, and treated human brown adipocytes with H O to dissect the direct effect of oxidative stress in vitro. Read More

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http://dx.doi.org/10.1111/febs.14838DOI Listing
April 2019
1 Read

Rev1 plays central roles in mammalian DNA-damage tolerance in response to UV irradiation.

FEBS J 2019 Apr 8. Epub 2019 Apr 8.

Beijing Key Laboratory of DNA Damage Responses and College of Life Sciences, Capital Normal University, Beijing, China.

Rev1, a Y-family DNA polymerase, is involved in the tolerance of DNA damage by translesion DNA synthesis (TLS). Previous studies have shown that the C-terminal domain (CTD) and ubiquitin (Ub)-binding (UBM) domains of Rev1 play important roles in UV-damage tolerance, but how these domains contribute to the process remains unclear. In this study, we created Ub mutations in a proliferating cell nuclear antigen (PCNA)-Ub fusion that differentially affect its interaction with Rev1 and Polη and found that UV-damage tolerance depends on its interaction with Rev1 but not Polη. Read More

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http://dx.doi.org/10.1111/febs.14840DOI Listing

Stroma in normal and cancer wound healing.

FEBS J 2019 Apr 8. Epub 2019 Apr 8.

Molecular Diagnostics and Therapeutics Group, Charles Bell House, Division of Surgery and Interventional Science, University College London, UK.

It is currently believed that stroma, the connective framework of biological tissues, plays a central role in normal wound healing and in cancer. In both these contexts, stromal cellular components such as activated fibroblasts interact with complex protein networks that include growth factors, structural protein or proteinases in order to initiate and sustain an extensive remodelling process. However, although this process is usually spatially and temporally self-limited, it is unregulated in the case of cancer and leads to uncontrolled cell proliferation and invasion within tissues, metastasis and therapeutic resistance. Read More

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http://dx.doi.org/10.1111/febs.14842DOI Listing

R180T variant of δ-ornithine aminotransferase associated with gyrate atrophy: biochemical, computational, X-ray and NMR studies provide insight into its catalytic features.

FEBS J 2019 Apr 8. Epub 2019 Apr 8.

Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Italy.

Among the over 50 gyrate atrophy-causing mutations of ornithine δ-aminotransferase (OAT), the R180T involves an active site residue located at the dimer interface, which in the crystal structure of OAT complexed with 5-fluoromethylornithine engages a salt bridge with the α-carboxylate of the substrate analogue. Starting from the previous finding that no transaminase activity was detected in CHO-K cells expressing the R180T variant, here we try to shed light at the protein level on the structural and/or functional defects of the R180T variant. To this aim, the variant has been cloned, expressed, purified and characterized by a combination of biochemical and structural studies. Read More

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http://dx.doi.org/10.1111/febs.14843DOI Listing

MAVS-induced mitochondrial membrane remodeling.

FEBS J 2019 Apr 8;286(8):1540-1542. Epub 2019 Apr 8.

Interfaculty Institute of Biochemistry, Eberhard Karls University, Tübingen, Germany.

Mitochondrial membrane remodeling has been linked with several cellular processes including mitochondrial damage or apoptotic cell death and proceeds via yet poorly understood mechanisms. In this issue of The FEBS Journal, Hwang et al. used different forms of super resolution microscopy to study the formation of macromolecular complexes of mitochondrial antiviral signal protein (MAVS). Read More

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http://dx.doi.org/10.1111/febs.14822DOI Listing

Molecular insights into the role of the polyalanine region in mediating PHOX2B aggregation.

FEBS J 2019 Apr 7. Epub 2019 Apr 7.

Institute of Biostructure and Bioimaging, CNR, Napoli, Italy.

About 90% of congenital central hypoventilation syndrome (CCHS) patients show polyalanine triplet expansions in the coding region of transcription factor PHOX2B, which renders this protein an intriguing target to understand the insurgence of this syndrome and for the design of a novel therapeutical approach. Consistently with the role of PHOX2B as a transcriptional regulator, it is reasonable that a general transcriptional dysregulation caused by the polyalanine expansion might represent an important mechanism underlying CCHS pathogenesis. Therefore, this study focused on the biochemical characterization of different PHOX2B variants, such as a variant containing the correct C-terminal (20 alanines) stretch, one of the most frequent polyalanine expansions (+7 alanines), and a variant lacking the complete alanine stretch (0 alanines). Read More

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http://dx.doi.org/10.1111/febs.14841DOI Listing
April 2019
2 Reads

Role of elastin peptides and elastin receptor complex in metabolic and cardiovascular diseases.

FEBS J 2019 Apr 4. Epub 2019 Apr 4.

UMR CNRS 7369 MEDyC, SFR CAP-Santé, Université de Reims Champagne-Ardenne, France.

The Cardiovascular Continuum describes a sequence of events from cardiovascular risk factors to end-stage heart disease. It includes conventional pathologies affecting cardiovascular functions such as hypertension, atherosclerosis or thrombosis and was traditionally considered from the metabolic point of view. This Cardiovascular Continuum, originally described by Dzau and Braunwald, was extended by O'Rourke to consider also the crucial role played by degradation of elastic fibers, occurring during aging, in the appearance of vascular stiffness, another deleterious risk factor of the continuum. Read More

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http://dx.doi.org/10.1111/febs.14836DOI Listing
April 2019
14 Reads

New views on an old enzyme: allosteric regulation and evolution of archaeal pyruvate kinases.

FEBS J 2019 Apr 4. Epub 2019 Apr 4.

Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universität Kiel, Kiel, Germany.

Pyruvate kinases (PKs) synthesize ATP as the final step of glycolysis in the three domains of life. PKs from most bacteria and eukarya are allosteric enzymes that are activated by sugar phosphates, e.g. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/febs.14837
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http://dx.doi.org/10.1111/febs.14837DOI Listing
April 2019
12 Reads

MicroRNA-520c-3p functions as a novel tumor suppressor in lung adenocarcinoma.

FEBS J 2019 Apr 3. Epub 2019 Apr 3.

Department of Molecular Imaging and Nuclear Medicine, Tianjin Medical University Cancer Institute and Hospital, China.

Lung cancer is a malignancy with one of the highest incidence rates, and it is the leading cause of cancer-related death. To gain further insights into the underlying mechanisms of tumor growth and metastasis, we investigated the role and expression of microRNAs in lung adenocarcinoma (LUAD). We discovered a significantly lower expression level of microRNA-520c-3p (miR-520c-3p) in LUAD tissues than in nontumor tissues. Read More

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http://dx.doi.org/10.1111/febs.14835DOI Listing
April 2019
4 Reads

Variable cytoplasmic actin expression impacts the sensitivity of different dystrophin-deficient mdx skeletal muscles to eccentric contraction.

FEBS J 2019 Apr 3. Epub 2019 Apr 3.

Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA.

Eccentric contractions (ECCs) induce force loss in several skeletal muscles of dystrophin-deficient mice (mdx), with the exception of the soleus (Sol). The eccentric force : isometric force (ECC : ISO), expression level of utrophin, fiber type distribution, and sarcoendoplasmic reticulum calcium ATPase expression are factors that differ between muscles and may contribute to the sensitivity of mdx skeletal muscle to ECC. Here, we confirm that the Sol of mdx mice loses only 13% force compared to 87% in the extensor digitorum longus (EDL) following 10 ECC of isolated muscles. Read More

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http://dx.doi.org/10.1111/febs.14831DOI Listing
April 2019
2 Reads

Studies on differentiation-dependent expression and activity of distinct transglutaminases by specific substrate peptides using three-dimensional reconstituted epidermis.

FEBS J 2019 Apr 3. Epub 2019 Apr 3.

Graduate School of Pharmaceutical Sciences, Nagoya University, Japan.

During skin formation, particularly during differentiation of keratinocytes, unique post-translational modifications play a role in forming a proteinaceous supermolecule called the cornified envelope (CE), which is necessary for barrier function. Transglutaminases (TGs) are essential enzymes involved in the cross-linking of various keratinocyte structural proteins to complete CE formation. The TG family consists of eight isozymes, with two members, TG1 and TG3, located mainly in the epidermis. Read More

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http://dx.doi.org/10.1111/febs.14832DOI Listing
April 2019
1 Read

Crystal structure and activation mechanism of DR3 death domain.

FEBS J 2019 Apr 3. Epub 2019 Apr 3.

Hefei National Laboratory for Physical Sciences at Microscale, CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, Anhui, China.

Death receptor 3 (DR3) (a.k.a. Read More

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http://dx.doi.org/10.1111/febs.14834DOI Listing
April 2019
4 Reads

CFP-1 interacts with HDAC1/2 complexes in C. elegans development.

FEBS J 2019 Apr 3. Epub 2019 Apr 3.

School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, UK.

CXXC finger binding protein 1 (CFP-1) is an evolutionarily conserved protein that binds to non-methylated CpG-rich promoters in mammals and Caenorhabditis elegans. This conserved epigenetic regulator is part of the COMPASS complex that contains the H3K4me3 methyltransferase SET1 in mammals and SET-2 in C. elegans. Read More

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http://dx.doi.org/10.1111/febs.14833DOI Listing
April 2019
2 Reads

Insight into the role of chondroitin sulfate E in angiogenesis.

FEBS J 2019 Apr 1. Epub 2019 Apr 1.

Laboratory of Molecular Pharmacology, Department of Pharmacy, University of Patras, Greece.

Chondroitin sulfate E (CS-E) is a glycosaminoglycan containing type-E disaccharide units (sulfated at C-4 and C-6 of N-acetylgalactosamine). CS-E is covalently linked to a core protein to form chondroitin sulfate proteoglycans (PGs) that are secreted or associated with the plasma membrane of several types of cells. CS-E-containing PGs selectively interact with growth factors and chemokines and control various cellular and/or tissue processes. Read More

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http://dx.doi.org/10.1111/febs.14830DOI Listing
April 2019
1 Read

Structures and interactions of syndecans.

FEBS J 2019 Apr 1. Epub 2019 Apr 1.

ICBMS, UMR 5246 CNRS - University Lyon 1, Univ Lyon, Villeurbanne, France.

The four syndecans identified in mammals are membrane proteoglycans that play major roles in regulating cell behavior, cell signaling, and cell-matrix interactions. The membrane forms of these syndecans function as receptors and co-receptors. Their ectodomains, which are proteolytically released in the extracellular matrix by shedding, also regulate various biological processes. Read More

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http://dx.doi.org/10.1111/febs.14828DOI Listing
April 2019
1 Read

MicroRNA 10a induces glioma tumorigenesis by targeting myotubularin-related protein 3 and regulating the Wnt/β-catenin signaling pathway.

FEBS J 2019 Mar 30. Epub 2019 Mar 30.

Department of Clinical Laboratory, Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases, Tianjin Huan Hu Hospital, China.

MicroRNAs are involved in the regulation of tumor formation. A previous study suggested that miR-10a promotes glioma cell migration and invasion. However, the effect of miR-10a on the proliferation of glioma cells remains unknown. Read More

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http://dx.doi.org/10.1111/febs.14824DOI Listing
March 2019
2 Reads
4.001 Impact Factor

Long non-coding RNAs in ocular diseases: new and potential therapeutic targets.

FEBS J 2019 Mar 30. Epub 2019 Mar 30.

Department of Inspection, The Medical Faculty of Qingdao University, China.

Long non-coding RNAs (lncRNAs) are non-protein coding transcripts containing more than 200 nucleotides. In the past, lncRNAs were considered as 'transcript noise' or 'pseudogenes' and were thus ignored. However, in recent years, lncRNAs have been proven to regulate gene expression at the epigenetic, transcriptional and translational level, and thereby influence cell proliferation, apoptosis, viability, immune response and oxidative stress. Read More

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http://dx.doi.org/10.1111/febs.14827DOI Listing
March 2019
4 Reads

A high-throughput screening for inhibitors of riboflavin synthase identifies novel antimicrobial compounds to treat brucellosis.

FEBS J 2019 Mar 30. Epub 2019 Mar 30.

Fundación Instituto Leloir, IIBBA-CONICET, Buenos Aires, Argentina.

Brucella spp. are pathogenic intracellular Gram-negative bacteria adapted to life within cells of several mammals, including humans. These bacteria are the causative agent of brucellosis, one of the zoonotic infections with the highest incidence in the world and for which a human vaccine is still unavailable. Read More

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http://dx.doi.org/10.1111/febs.14829DOI Listing
March 2019
1 Read

Stress - (self) eating: Epigenetic regulation of autophagy in response to psychological stress.

FEBS J 2019 Mar 30. Epub 2019 Mar 30.

National Centre for Cell Science, Savitribai Phule Pune University, Pune, India.

Autophagy is a constitutive and cytoprotective catabolic process. Aberrations in autophagy lead to a multitude of degenerative disorders, with neurodegeneration being one of the most widely studied autophagy-related disorders. While the field has largely been focusing on the cytosolic constituents and processes of autophagy, recent studies are increasingly appreciating the role of chromatin modifications and epigenetic regulation in autophagy maintenance. Read More

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http://dx.doi.org/10.1111/febs.14826DOI Listing
March 2019
3 Reads

Mechanistic insights into Nav1.7-dependent regulation of rat prostate cancer cell invasiveness revealed by toxin probes and proteomic analysis.

FEBS J 2019 Mar 29. Epub 2019 Mar 29.

The National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha, Hunan, China.

Voltage-gated sodium channels are involved in tumor metastasis, as potentiating or attenuating their activities affects the migration and invasion process of tumor cells. In the present study, we tested the effect of two peptide toxins, JZTX-I and HNTX-III which function as Nav1.7 activator and inhibitor, respectively, on the migration and invasion ability of prostate cancer (PCa) cell line Mat-LyLu. Read More

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http://dx.doi.org/10.1111/febs.14823DOI Listing

Long noncoding RNA ES1 controls the proliferation of breast cancer cells by regulating the Oct4/Sox2/miR-302 axis.

FEBS J 2019 Mar 29. Epub 2019 Mar 29.

Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.

ES1 is a long noncoding RNA (lncRNA) that regulates pluripotency of human embryonic stem cells, which is known to be a downstream target of stemness factors Oct4 and Nanog, and serves as a modular scaffold for Sox2. However, the role of ES1 in cancer biology is not fully characterized. The results of our study show that ES1 transcript is upregulated in both high-grade and P53-mutated breast tumor tissues. Read More

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http://dx.doi.org/10.1111/febs.14825DOI Listing
March 2019
1 Read

The acute myeloid leukemia-associated Nucleophosmin 1 gene mutations dictate amyloidogenicity of the C-terminal domain.

FEBS J 2019 Mar 28. Epub 2019 Mar 28.

Department of Pharmacy, CIRPEB: Centro Interuniversitario di Ricerca sui Peptidi Bioattivi, University of Naples "Federico II", Italy.

Nucleophosmin 1 (NPM1) is a nucleus-cytoplasm shuttling protein ubiquitously expressed and highly conserved. It is involved in many cellular processes and its gene is mutated in ~ 50-60% of Acute Myeloid Leukemia (AML) patients. These mutations cause its cytoplasmic mislocation and accumulation (referred to as NPM1c+) and open the door to rational targeted therapy for AML diseases with mutated NPM1. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/febs.14815
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http://dx.doi.org/10.1111/febs.14815DOI Listing
March 2019
2 Reads

The anti-prion RNA aptamer R12 disrupts the Alzheimer's disease-related complex between prion and amyloid β.

FEBS J 2019 Mar 27. Epub 2019 Mar 27.

Institute of Advanced Energy, Kyoto University, Uji, Kyoto, Japan.

The neurodegenerative disorder Alzheimer's disease (AD) is associated with the accumulation of misfolded proteins. Some recent studies suggested that amyloid beta (Aβ) forms soluble oligomers, protofibrils, and fibrils; the Aβ oligomers being more toxic than the fibrils. Surprisingly, these Aβ oligomers reportedly bind to prion protein (PrP), which acts as a receptor on the cell membrane, possibly resulting in AD. Read More

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http://dx.doi.org/10.1111/febs.14819DOI Listing
March 2019
3 Reads

Linear double-stranded DNAs as innovative biological parts to implement genetic circuits in mammalian cells.

FEBS J 2019 Mar 26. Epub 2019 Mar 26.

Department of Colorectal Surgery, Tianjin Union Medical Center, China.

Synthetic biology employs engineering principles to redesign biological systems for biomedical or industrial purposes. Innovation and application of original biological parts for genetic circuit construction will significantly facilitate and expedite the development of synthetic biology. Here, we built two- or three-input linear double-stranded DNA (ldsDNA)-based Boolean AND gate genetic circuits in mammalian cells. Read More

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http://dx.doi.org/10.1111/febs.14816DOI Listing
March 2019
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4.001 Impact Factor

The extracellular matrix as a multitasking player in disease.

FEBS J 2019 Mar 25. Epub 2019 Mar 25.

Biochemistry, Biochemical Analysis & Matrix Pathobiochemistry Research Group, Laboratory of Biochemistry, Department of Chemistry, University of Patras, Greece.

Extracellular matrices (ECMs) are highly specialized and dynamic three-dimensional (3D) scaffolds into which cells reside in tissues. ECM is composed of a variety of fibrillar components, such as collagens, fibronectin, and elastin, and non-fibrillar molecules as proteoglycans, hyaluronan, and glycoproteins including matricellular proteins. These macromolecular components are interconnected forming complex networks that actively communicate with cells through binding to cell surface receptors and/or matrix effectors. Read More

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http://dx.doi.org/10.1111/febs.14818DOI Listing
March 2019
4.001 Impact Factor

Structure and characterization of Aspergillus fumigatus lipase B with a unique, oversized regulatory subdomain.

FEBS J 2019 Mar 25. Epub 2019 Mar 25.

School of Food Science and Engineering, South China University of Technology, Guangzhou, China.

Fungal lipases are efficient and environment-friendly biocatalysts for many industrially relevant processes. One of the most widely applied lipases in the manufacturing industry is Candida antarctica lipase B (CALB). Here, we report the biochemical and structural characterization of a novel CALB-like lipase from an important human pathogen-Aspergillus fumigatus (AFLB), which has high sn-1,3-specificity toward triolein. Read More

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http://dx.doi.org/10.1111/febs.14814DOI Listing

Molecular and structural characterization of a TEAD mutation at the origin of Sveinsson's chorioretinal atrophy.

FEBS J 2019 Mar 23. Epub 2019 Mar 23.

Disease Area Oncology, Novartis Institutes for Biomedical Research, Basel, Switzerland.

Four TEAD transcription factors (TEAD1-4) mediate the signalling output of the Hippo pathway that controls organ size in humans. TEAD transcriptional activity is regulated via interactions with the YAP, TAZ and VGLL proteins. A mutation in the TEAD1 gene, Tyr421His, has been identified in patients suffering from Sveinsson's chorioretinal atrophy (SCA), an autosomal dominant eye disease. Read More

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http://dx.doi.org/10.1111/febs.14817DOI Listing

Modeling therapy resistance via the EGFR signaling pathway.

FEBS J 2019 Apr 20;286(7):1284-1286. Epub 2019 Mar 20.

Division of Bioinformatics, Biocenter, Medical University of Innsbruck, Austria.

Mutations in KRAS are often associated with resistance to EGFR-targeting antibody therapy. Using comprehensive systems analyses, GNB5 has been identified as a potential target to overcome therapy resistance targeting the EGFR signaling pathways, whereby the AKT signaling pathway (PI3K) rather than the ERK signaling pathway (RAS) might be dominantly affected. Personalized mathematical modeling and simulations of this signaling pathway/network and respective perturbations are of great utility to customize therapy for patients. Read More

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http://dx.doi.org/10.1111/febs.14809DOI Listing

Dynamic analysis of proteomic alterations in response to N-linked glycosylation inhibition in a drug-resistant ovarian carcinoma cell line.

FEBS J 2019 Apr 2;286(8):1594-1605. Epub 2019 Apr 2.

College of Life Sciences, Northwest University, Xi'an, China.

Glycosylation inhibition can improve the efficacy of antitumor drugs and enhance the apoptosis of cancer cells, thus holding great potential for cancer treatment. Inhibition of N-glycosylation induces endoplasmic reticulum (ER) stress and the unfolded protein response (UPR), and eventually triggers ER stress-related apoptosis. Unfortunately, the detailed timeline of these cell responses and protein expression alterations related to N-glycosylation inhibition is not explicit yet, and the pathways involved in different stages of N-glycosylation inhibition still need to be characterized. Read More

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http://dx.doi.org/10.1111/febs.14811DOI Listing
April 2019
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Glutathione S-transferase omega 1 inhibition activates JNK-mediated apoptotic response in breast cancer stem cells.

FEBS J 2019 Mar 15. Epub 2019 Mar 15.

Centre for Chemical Biology, CSIR-Indian Institute of Chemical Technology, Hyderabad, India.

Glutathione S-transferase omega 1 (GSTO1) contributes to the inactivation of a wide range of drug compounds via conjugation to glutathione during phase reactions. Chemotherapy-induced GSTO1 expression in breast cancer cells leads to chemoresistance and promotes metastasis. In search of novel GSTO1 inhibitors, we identified S2E, a thia-Michael adduct of sulfonamide chalcone with low LC (3. Read More

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http://dx.doi.org/10.1111/febs.14813DOI Listing

Borrelia outer surface protein C is capable of human fibrinogen binding.

FEBS J 2019 Mar 15. Epub 2019 Mar 15.

Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.

Outer surface protein C (OspC) is one of the most abundant surface lipoproteins produced during early infection by the Borrelia spirochete, the causative agent of Lyme disease. The high sequence variability of the ospC gene results in the production of several and strongly divergent OspC types. One of the known roles of OspC is the recruitment of blood components, including complement regulators, to facilitate the bloodstream survival of Borrelia at an essential stage of host infection. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/febs.14810
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http://dx.doi.org/10.1111/febs.14810DOI Listing
March 2019
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Differential regulation of myc homologs by Wnt/β-Catenin signaling in the early metazoan Hydra.

FEBS J 2019 Mar 14. Epub 2019 Mar 14.

Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Austria.

The c-Myc protein is a transcription factor with oncogenic potential controlling fundamental cellular processes. Homologs of the human c-myc protooncogene have been identified in the early diploblastic cnidarian Hydra (myc1, myc2). The ancestral Myc1 and Myc2 proteins display the principal design and biochemical properties of their vertebrate derivatives, suggesting that important Myc functions arose very early in metazoan evolution. Read More

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http://dx.doi.org/10.1111/febs.14812DOI Listing

SKA1 induces de novo MTX-resistance in osteosarcoma through inhibiting FPGS transcription.

FEBS J 2019 Mar 9. Epub 2019 Mar 9.

Department of Oncology, Affiliated 6th People's Hospital, Shanghai Jiaotong University, China.

De novo methotrexate (MTX)-resistance, whose underlying mechanism remains largely unknown, usually leads to very poor prognosis in patients with osteosarcoma (OS). In this study, we established the de novo MTX-resistant OS cell line SF-86 and identified the candidate gene spindle and kinetochore associated complex subunit 1 (SKA1) as potentially related to de novo MTX-resistance. Analysis of a cohort of 95 OS patients demonstrated that SKA1 overexpression significantly correlated with de novo MTX-resistance and poor 5-year survival. Read More

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http://dx.doi.org/10.1111/febs.14808DOI Listing

Threshold protective effect of deuterated polyunsaturated fatty acids on peroxidation of lipid bilayers.

FEBS J 2019 Mar 9. Epub 2019 Mar 9.

Retrotope, Inc., Los Altos, CA, USA.

Autoxidation of polyunsaturated fatty acids (PUFAs) damages lipid membranes and generates numerous toxic by-products implicated in neurodegeneration, aging, and other pathologies. Abstraction of bis-allylic hydrogen atoms is the rate-limiting step of PUFA autoxidation, which is inhibited by replacing bis-allylic hydrogens with deuterium atoms (D-PUFAs). In cells, the presence of a relatively small fraction of D-PUFAs among natural PUFAs is sufficient to effectively inhibit lipid peroxidation (LPO). Read More

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http://doi.wiley.com/10.1111/febs.14807
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http://dx.doi.org/10.1111/febs.14807DOI Listing
March 2019
5 Reads