6,270 results match your criteria FEBS Journal[Journal]


Discovery and structural analysis of a phloretin hydrolase from the opportunistic human pathogen Mycobacterium abscessus.

FEBS J 2019 Feb 19. Epub 2019 Feb 19.

Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, 730000, P.R. China.

The family of PhlG proteins catalyze the hydrolysis of carbon-carbon bonds and are widely distributed across diverse bacterial species. Two members of the PhlG family have been separately identified as 2,4-diacetylphloroglucinol hydrolase and phloretin hydrolase; however, the extent of functional divergence and catalytic substrates for most members of this family is still unknown. Here, using sequence similarity network and gene co-occurrence analysis, we categorized PhlG proteins into several subgroups and inferred that PhlG proteins from Mycobacterium abscessus (MaPhlG) are likely to be functionally equivalent to phloretin hydrolase. Read More

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http://doi.wiley.com/10.1111/febs.14792
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http://dx.doi.org/10.1111/febs.14792DOI Listing
February 2019
1 Read

Control and regulation of S-Adenosylmethionine biosynthesis by the regulatory β subunit and quinolone-based compounds.

FEBS J 2019 Feb 18. Epub 2019 Feb 18.

Molecular Biophysics Group, Institute of Integrative Biology, Faculty of Health and Life Sciences, University of Liverpool, L69 7ZB, UK.

Methylation is an underpinning process of life and provides control for biological processes such as DNA synthesis, cell growth and apoptosis. Methionine Adenosyltransferases (MAT) produce the cellular methyl donor, S-Adenosylmethionine (SAMe). Dysregulation of SAMe level is a relevant event in many diseases, including cancers such as hepatocellular carcinoma and colon cancer. Read More

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http://dx.doi.org/10.1111/febs.14790DOI Listing
February 2019

Breaking down chronic inflammatory diseases: the role of biglycan in promoting a switch between inflammation and autophagy.

FEBS J 2019 Feb 18. Epub 2019 Feb 18.

Pharmazentrum Frankfurt/ZAFES, Institut für Allgemeine Pharmakologie und Toxikologie, Klinikum der Goethe-Universität, Theodor-Stern-Kai 7, 60590, Frankfurt am Main, Germany.

It is well established that biglycan, a small leucine-rich proteoglycan, acts as an extracellular matrix-derived danger signal in its soluble form. By binding to innate immunity Toll-like receptors (TLR) 2 and 4, biglycan initiates and perpetuates the inflammatory response. Previous work has conveyed that biglycan's role in inflammation extends far beyond its function as a canonical danger signal. Read More

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http://dx.doi.org/10.1111/febs.14791DOI Listing
February 2019

Tyrosine metabolism: identification of a key residue in the acquisition of prephenate aminotransferase activity by 1β aspartate aminotransferase.

FEBS J 2019 Feb 16. Epub 2019 Feb 16.

Univ. Grenoble Alpes, INRA, CNRS, CEA, BIG-LPCV, 38000, Grenoble, France.

Alternative routes for the post-chorismate branch of the biosynthetic pathway leading to tyrosine exist, the 4-hydroxyphenylpyruvate or the arogenate route. The arogenate route involves the transamination of prephenate into arogenate. In a previous study, we found that, depending on the microorganisms possessing the arogenate route, three different aminotransferases evolved to perform prephenate transamination i. Read More

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http://dx.doi.org/10.1111/febs.14789DOI Listing
February 2019

A novel long noncoding RNA regulates the immune response in MAC-T cells and contributes to bovine mastitis.

FEBS J 2019 Feb 16. Epub 2019 Feb 16.

College of Veterinary Medicine, Northwest A&F University, Yangling, China.

The Long noncoding RNAs (lncRNAs) are known to transcriptionally regulate a wide spectrum of diseases. Here, we screened for potentially functional lncRNAs in a mammary epithelial cell model of bovine mastitis by using RNA-Seq technology and identified a class of previously undetected mastitis-related lncRNAs. A novel lncRNA was widely expressed in a variety of bovine tissues with diverse relative abundance and had a relatively low expression in mammary tissue. Read More

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http://dx.doi.org/10.1111/febs.14783DOI Listing
February 2019

The C-terminal domain of HpDprA is a DNA-binding Winged Helix domain that does not bind double-stranded DNA.

FEBS J 2019 Feb 16. Epub 2019 Feb 16.

Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, 1 Avenue de la Terrasse, F-91198, Gif-sur-Yvette, France.

DprA, a ubiquitous multidomain DNA-binding protein, plays a crucial role during natural transformation in bacteria. Here, we carried out the structural analysis of DprA from the human pathogen Helicobacter pylori by combining data issued from the 1.8 Å resolution X-ray structure of the Pfam02481 domain dimer (RF), the NMR structure of the carboxy-terminal domain (CTD) and the low-resolution structure of the full-length DprA dimer obtained in solution by SAXS. Read More

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http://dx.doi.org/10.1111/febs.14788DOI Listing
February 2019

Crystal structures of human 17β-hydroxysteroid dehydrogenase type 1 complexed with estrone and NADP reveal the mechanism of substrate inhibition.

FEBS J 2019 Feb 15. Epub 2019 Feb 15.

CHU de Québec Research Center and Department of Molecular Medicine, Laval University, Québec, QC, Canada.

Human 17β-hydroxysteroid dehydrogenase type 1 (17β-HSD1) catalyzes the last step in estrogen activation and is thus involved in estrogen-dependent diseases (EDDs). Unlike other 17β-HSD members, 17β-HSD1 undergoes a significant substrate-induced inhibition that we have previously reported. Here we solved the binary and ternary crystal structures of 17β-HSD1 in complex with estrone and cofactor analog NADP , demonstrating critical enzyme-substrate-cofactor interactions. Read More

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http://dx.doi.org/10.1111/febs.14784DOI Listing
February 2019

Congenital myopathy-related mutations in tropomyosin disrupt regulatory function through altered actin affinity and tropomodulin binding.

FEBS J 2019 Feb 15. Epub 2019 Feb 15.

Department of Physics, University at Buffalo, SUNY, Buffalo, NY, USA.

Tropomyosin binds along actin filaments and regulates myosin binding to control muscle contraction. Tropomodulin binds to the pointed end of a filament and regulates actin dynamics, which maintains the length of a thin filament. To define the structural determinants of these tropomyosin functions, we examined the effects of two congenital myopathy mutations, A4V and R91C, in the tropomyosin gene, TPM3, which encodes the tropomyosin isoform, Tpm3. Read More

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http://dx.doi.org/10.1111/febs.14787DOI Listing
February 2019

Mitochondrial metabolism is inhibited by the HIF1α-MYC-PGC-1β axis in BRAF V600E thyroid cancer.

FEBS J 2019 Feb 15. Epub 2019 Feb 15.

Center for Vascular Biology, Institute for Translational Medicine, College of Medicine, Qingdao University, Qingdao, 266021, P.R. China.

BRAF V600E is the most common mutation identified in thyroid cancers. However, the relationship between BRAF V600E and metabolic reprogramming in thyroid cancer is unclear. Here, we investigate the mechanism of metabolic reprogramming in BRAF V600E thyroid cancer by constructing BRAF V600E-overexpressing and BRAF-knockdown thyroid cell lines for use in mitochondrial respiration and glycolysis experiments. Read More

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http://dx.doi.org/10.1111/febs.14786DOI Listing
February 2019
1 Read
4.001 Impact Factor

Msc6p is required for mitochondrial translation initiation in the absence of formylated Met-tRNAf.

FEBS J 2019 Feb 15. Epub 2019 Feb 15.

Departamento de Microbiologia - Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil.

Mitochondrial translation normally requires formylation of the initiator tRNA-met, a reaction catalyzed by the enzyme formyl transferase, Fmt1p and MTFMT in Saccharomyces cerevisiae and human mitochondria, respectively. Yeast fmt1 mutants devoid of Fmt1p, however, can synthesize all mitochondrial gene products by initiating translation with a non-formylated methionyl tRNA. Yeast synthetic respiratory-deficient fmt1 mutants have uncovered several factors suggested to play a role in translation initiation with non-formylated methionyl tRNA. Read More

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http://dx.doi.org/10.1111/febs.14785DOI Listing
February 2019
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Structural, biochemical and biophysical characterization of recombinant human fumarate hydratase.

FEBS J 2019 Feb 14. Epub 2019 Feb 14.

Laboratório de Cristalografia de Proteínas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14040-903, Brazil.

Fumarate hydratases (FHs, fumarases) catalyze the reversible conversion of fumarate into L-malate. FHs are distributed over all organisms and play important roles in energy production, DNA repair and as tumor suppressors. They are very important targets both in the study of human metabolic disorders and as potential therapeutic targets in neglected tropical diseases and tuberculosis. Read More

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http://dx.doi.org/10.1111/febs.14782DOI Listing
February 2019
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Structural insights into the unique polylactate-degrading mechanism of Thermobifida alba cutinase.

FEBS J 2019 Feb 14. Epub 2019 Feb 14.

Graduate School of Comprehensive Rehabilitation, College of Health and Human Sciences, Osaka Prefecture University, 3-7-30 Habikino, Habikino 583-8555, Osaka, Japan.

Cutinases are enzymes known to degrade polyester-type plastics. Est119, a plastic-degrading type of cutinase from Thermobifida alba AHK119 (herein called Ta_cut), shows a broad substrate specificity toward polyesters, and can degrade substrates including polylactic acid (PLA). However, the PLA-degrading mechanism of cutinases is still poorly understood. Read More

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http://dx.doi.org/10.1111/febs.14781DOI Listing
February 2019

Long Non-Coding RNA Crnde Attenuates Cardiac Fibrosis via Smad3-Crnde Negative Feedback in Diabetic Cardiomyopathy.

FEBS J 2019 Feb 12. Epub 2019 Feb 12.

Department of Pharmacy, The General Hospital of Western Theater Command, Chengdu, 610083, China.

Diabetic cardiomyopathy (DCM) - ventricular dysfunction in the absence of underlying heart disease - is a common complication of diabetes and a leading cause of mortality associated with the disease. In DCM, cardiac fibrosis is the main cause of heart failure (HF). Although it is well-established that the transforming growth factor-beta (TGF-β) signaling pathway plays a part in inducing cardiac fibrosis in DCM, details of the molecular mechanism involved remain elusive. Read More

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http://dx.doi.org/10.1111/febs.14780DOI Listing
February 2019

Carbonic anhydrases are involved in mitochondrial biogenesis and control the production of lactate by human Sertoli cells.

FEBS J 2019 Feb 6. Epub 2019 Feb 6.

Department of Microscopy, Laboratory of Cell Biology, Institute of Biomedical Sciences, Abel Salazar (ICBAS) and Multidisciplinary Unit for Biomedical Research (UMIB), University of Porto, Portugal.

The process that allows cells to control their pH and bicarbonate levels is essential for ionic and metabolic equilibrium. Carbonic anhydrases (CAs) catalyse the conversion of CO to and H and are thus essential for this process. Herein, we inhibited CAs with acetazolamide - ACT and SLC-0111 - to study their involvement in the metabolism, mitochondrial potential, mitochondrial biogenesis and lipid metabolism of human Sertoli cells (hSCs), obtained from biopsies from men with conserved spermatogenesis. Read More

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http://dx.doi.org/10.1111/febs.14779DOI Listing
February 2019
2 Reads

Serine/arginine protein-specific kinase 2 promotes the development and progression of pancreatic cancer by downregulating Numb and p53.

FEBS J 2019 Feb 6. Epub 2019 Feb 6.

Department of Gastrointestinal Surgery, The First Hospital, China Medical University, Shenyang, China.

Serine/arginine protein specific kinase 2 (SRPK2) plays a vital role in the progression of a range of different malignancies, including pancreatic cancer. However, the mechanisms are poorly understood. Previous studies have shown that in hepatocellular carcinoma, SRPK2 knockdown leads to upregulation of the cell fate determining protein Numb, and in pancreatic cancer cells, Numb knockdown prevents ubiquitin-mediated degradation of p53. Read More

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http://dx.doi.org/10.1111/febs.14778DOI Listing
February 2019

Hyaluronan: molecular size-dependent signaling and biological functions in inflammation and cancer.

FEBS J 2019 Feb 6. Epub 2019 Feb 6.

Biochemistry, Biochemical Analysis & Matrix Pathobiology Research Group, Laboratory of Biochemistry, Department of Chemistry, University of Patras, Greece.

Hyaluronan (HA) is a linear nonsulfated glycosaminoglycan of the extracellular matrix that plays a pivotal role in a variety of biological processes. High-molecular weight HA exhibits different biological properties than oligomers and low-molecular weight HA. Depending on their molecular size, HA fragments can influence cellular behavior in a different mode of action. Read More

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http://dx.doi.org/10.1111/febs.14777DOI Listing
February 2019

Systems analysis identifies potential target genes to overcome cetuximab resistance in colorectal cancer cells.

FEBS J 2019 Feb 4. Epub 2019 Feb 4.

Laboratory for Systems Biology and Bio-inspired Engineering, Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea.

Cetuximab (CTX), a monoclonal antibody against epidermal growth factor receptor, is being widely used for colorectal cancer (CRC) with wild-type (WT) KRAS. However, its responsiveness is still very limited and WT KRAS is not enough to indicate such responsiveness. Here, by analyzing the gene expression data of CRC patients treated with CTX monotherapy, we have identified DUSP4, ETV5, GNB5, NT5E, and PHLDA1 as potential targets to overcome CTX resistance. Read More

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http://dx.doi.org/10.1111/febs.14773DOI Listing
February 2019
2 Reads

A subset of calcium-binding S100 proteins show preferential heterodimerization.

FEBS J 2019 Feb 4. Epub 2019 Feb 4.

Department of Biochemistry, The University of Western Ontario, London, Canada.

The assembly of proteins into dimers and oligomers is a necessary step for the proper function of transcription factors, muscle proteins, and proteases. In uncontrolled states, oligomerization can also contribute to illnesses such as Alzheimer's disease. The S100 protein family is a group of dimeric proteins that have important roles in enzyme regulation, cell membrane repair, and cell growth. Read More

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http://dx.doi.org/10.1111/febs.14775DOI Listing
February 2019
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IRE1α is critical for Kaempferol-induced neuroblastoma differentiation.

FEBS J 2019 Feb 4. Epub 2019 Feb 4.

Apoptosis and Cell Survival Research Lab, Department of Biosciences, School of Biosciences and Technology, VIT University, Vellore, India.

Neuroblastoma is an embryonic malignancy that arises out of the neural crest cells of the sympathetic nervous system. It is the most common childhood tumor known for its spontaneous regression via the process of differentiation. The induction of differentiation using small molecules such as retinoic acid is one of the therapeutic strategies to treat the residual disease. Read More

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http://dx.doi.org/10.1111/febs.14776DOI Listing
February 2019

A fluorinated indole-based MDM2 antagonist selectively inhibits the growth of p53 osteosarcoma cells.

FEBS J 2019 Feb 4. Epub 2019 Feb 4.

Faculty of Chemistry, Jagiellonian University, Krakow, Poland.

The p53 protein is engaged in the repair of DNA mutations and elimination of heavily damaged cells, providing anticancer protection. Dysregulation of p53 activity is a crucial step in carcinogenesis. This dysregulation is often caused by the overexpression of negative regulators of p53, among which MDM2 is the most prominent one. Read More

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http://dx.doi.org/10.1111/febs.14774DOI Listing
February 2019
1 Read

MAVS polymers smaller than 80 nm induce mitochondrial membrane remodeling and interferon signaling.

FEBS J 2019 Feb 4. Epub 2019 Feb 4.

Department of Medicine, University of Cambridge, MRC Laboratory of Molecular Biology, UK.

Double-stranded RNA (dsRNA) is a potent proinflammatory signature of viral infection and is sensed primarily by RIG-I-like receptors (RLRs). Oligomerization of RLRs following binding to cytosolic dsRNA activates and nucleates self-assembly of the mitochondrial antiviral-signaling protein (MAVS). In the current signaling model, the caspase recruitment domains of MAVS form helical fibrils that self-propagate like prions to promote signaling complex assembly. Read More

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http://dx.doi.org/10.1111/febs.14772DOI Listing
February 2019
1 Read
4.001 Impact Factor

Two aspartate residues close to the lesion binding site of Agrobacterium (6-4) photolyase are required for Mg stimulation of DNA repair.

FEBS J 2019 Jan 31. Epub 2019 Jan 31.

Botanical Institute, Karlsruhe Institute of Technology, Germany.

Prokaryotic (6-4) photolyases branch at the base of the evolution of cryptochromes and photolyases. Prototypical members contain an iron-sulphur cluster which was lost in the evolution of the other groups. In the Agrobacterium (6-4) photolyase PhrB, the repair of DNA lesions containing UV-induced (6-4) pyrimidine dimers is stimulated by Mg . Read More

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http://dx.doi.org/10.1111/febs.14770DOI Listing
January 2019

Simple high-resolution NMR spectroscopy as a tool in molecular biology.

FEBS J 2019 Feb 1. Epub 2019 Feb 1.

Department of Molecular and Cell Biology, Leicester Institute of Structural and Chemical Biology, University of Leicester, UK.

NMR is one of the major techniques for investigating the structure, dynamics and interactions between biomolecules. However, non-experts often experience NMR experimentation and data analysis as intimidating. We discuss a simple yet powerful NMR technique, the so-called chemical shift perturbation (CSP) analysis, as a tool to elucidate macromolecular interactions in small- and medium-sized complexes, including protein-protein, protein-drug, and protein-DNA/RNA interactions. Read More

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http://doi.wiley.com/10.1111/febs.14771
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http://dx.doi.org/10.1111/febs.14771DOI Listing
February 2019
2 Reads

Lipid phosphatases SKIP and SHIP2 regulate fibronectin-dependent cell migration in glioblastoma.

FEBS J 2019 Jan 29. Epub 2019 Jan 29.

IRIBHM, Université Libre de Bruxelles, Belgium.

Cell migration is an important process that occurs during development and has also been linked to the motility of cancer cells. Cytoskeleton reorganization takes place in the migration process leading to lamellipodia formation. Understanding the molecular underpinnings of cell migration is particularly important in studies of glioblastoma, a highly invasive and aggressive cancer type. Read More

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http://dx.doi.org/10.1111/febs.14769DOI Listing
January 2019

Urate-lowering therapy alleviates atherosclerosis inflammatory response factors and neointimal lesions in a mouse model of induced carotid atherosclerosis.

FEBS J 2019 Jan 28. Epub 2019 Jan 28.

Institute of Metabolic Diseases, Qingdao University, China.

Hyperuricemia (HU) is a cause of gout. Clinical studies show a link between HU and cardiovascular disease. However, the role of soluble serum urate (SU) on atherosclerosis development remains elusive. Read More

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http://dx.doi.org/10.1111/febs.14768DOI Listing
January 2019
1 Read

Regulation of mitochondrial ROS production by HIC-5: a common feature of oncogene-induced senescence and tumor invasiveness?

FEBS J 2019 Feb 25;286(3):456-458. Epub 2019 Jan 25.

Research Institute for Biomedical Aging Research, University of Innsbruck, Austria.

Transformation by the ras oncogene can result in promotion of metastasis as well as induction of senescence via increased tissue remodeling, for example, by matrix metalloproteases. Increased production of mitochondrial reactive oxygen species (mtROS) via NADPH oxidase 4 (NOX4) is implicated in this process. Hydrogen peroxide-inducible clone-5 (HIC-5) is postulated to sense both matrix detachment of transformed cells and intracellular ROS and can inhibit ras signaling via inhibition of NOX4. Read More

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http://dx.doi.org/10.1111/febs.14746DOI Listing
February 2019

Ice-binding proteins and the 'domain of unknown function' 3494 family.

FEBS J 2019 Jan 24. Epub 2019 Jan 24.

Department of Biotechnology and Biosciences, University of Milano-Bicocca, Italy.

Ice-binding proteins (IBPs) control the growth and shape of ice crystals to cope with subzero temperatures in psychrophilic and freeze-tolerant organisms. Recently, numerous proteins containing the domain of unknown function (DUF) 3494 were found to bind ice crystals and, hence, are classified as IBPs. DUF3494 IBPs constitute today the most widespread of the known IBP families. Read More

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http://dx.doi.org/10.1111/febs.14764DOI Listing
January 2019

Endoplasmic reticulum stress: a key player in human disease.

FEBS J 2019 Jan;286(2):228-231

Cambridge Institute for Medical Research (CIMR), University of Cambridge, UK.

This Special Issue comprises eleven excellent reviews that illustrate the role of ER stress in different human diseases, including myopathies and lung diseases, as well as in modulating liver dysfunction and inflammatory responses. These reviews also highlight the function of the UPR in neurodegenerative disorders and cancer, while discussing the potential benefits of targeting the UPR as a therapeutic approach. We hope you find these reviews interesting and informative and we thank the authors for these excellent contributions. Read More

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http://dx.doi.org/10.1111/febs.14740DOI Listing
January 2019

Circularized and solubility-enhanced MSPs facilitate simple and high-yield production of stable nanodiscs for studies of membrane proteins in solution.

FEBS J 2019 Jan 23. Epub 2019 Jan 23.

Structural Biophysics, Niels Bohr Institute, University of Copenhagen, Denmark.

Recently, an enzymatic reaction was utilized to covalently link the N and C termini of membrane scaffold proteins to produce circularized nanodiscs that were more homogeneous and stable than standard nanodiscs. We continue this development and aim for obtaining high yields of stable and monodisperse nanodiscs for structural studies of membrane proteins by solution small-angle scattering techniques. Based on the template MSP1E3D1, we designed an optimized membrane scaffold protein (His-lsMSP1E3D1) with a sortase recognition motif and high abundance of solubility-enhancing negative charges. Read More

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http://doi.wiley.com/10.1111/febs.14766
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http://dx.doi.org/10.1111/febs.14766DOI Listing
January 2019
3 Reads

The non-glycosylated N-terminal domain of human thrombopoietin is a molten globule under native conditions.

FEBS J 2019 Jan 23. Epub 2019 Jan 23.

Quantum Beam Science Center, Japan Atomic Energy Agency, Tokai, Japan.

Human thrombopoietin (hTPO) is a primary hematopoietic growth factor that regulates megakaryocytopoiesis and platelet production. The non-glycosylated form of 1-163 residues of hTPO (hTPO ) including the N-terminal active site domain (1-153 residues) is a candidate for treating thrombocytopenia. However, the autoantigenicity level of hTPO is higher than that of the full-length glycosylated hTPO (ghTPO ). Read More

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http://dx.doi.org/10.1111/febs.14765DOI Listing
January 2019

Loop size optimization induces a strong thermal stabilization of the thioredoxin fold.

FEBS J 2019 Jan 23. Epub 2019 Jan 23.

Institute of Biostructures and Bioimaging, C.N.R., Naples, Italy.

The definition of the structural basis of protein thermostability represents a major topic in structural biology and protein chemistry. We have recently observed that proteins isolated from thermophilic organisms show a better adherence to the fundamental rules of protein topology previously unveiled by Baker and coworkers (Koga et al. Nature. Read More

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http://dx.doi.org/10.1111/febs.14767DOI Listing
January 2019
1 Read

Identifying the binding mechanism of LEAP2 to receptor GHSR1a.

FEBS J 2019 Jan 22. Epub 2019 Jan 22.

Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China.

Liver-expressed antimicrobial peptide 2 (LEAP2) is a highly conserved secretory peptide first isolated in 2003. However, its exact biological functions remained elusive until a recent study identified it as an endogenous antagonist for the growth hormone secretagogue receptor (GHSR1a), a G protein-coupled receptor for which the gastric peptide ghrelin is the endogenous agonist. By tuning the ghrelin-GHSR1a system, LEAP2 has an important function in energy metabolism. Read More

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http://dx.doi.org/10.1111/febs.14763DOI Listing
January 2019
1 Read

Kinetic and mechanistic studies of p38α MAP kinase phosphorylation by MKK6.

FEBS J 2019 Jan 20. Epub 2019 Jan 20.

Key Laboratory of Ministry of Education for Protein Science, School of Life Sciences, Tsinghua University, Beijing, China.

Bistability (coexistence of two stable steady states in a dynamical system) is a key mechanism of cellular decision-making and has been observed in many biochemical reaction networks such as mitogen-activated protein kinase (MAPK) signaling pathways. Theoretical studies have shown that bistability can arise in a single two-site MAPK phosphorylation and dephosphorylation cycle. However, the bistable behavior mostly relies on the kinetic mechanisms and parameters of this two-site modification. Read More

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http://dx.doi.org/10.1111/febs.14762DOI Listing
January 2019
2 Reads

Ran1 is essential for parental macronuclear import of apoptosis-inducing factor and programmed nuclear death in Tetrahymena thermophila.

FEBS J 2019 Jan 20. Epub 2019 Jan 20.

Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, China.

During programmed nuclear death (PND), apoptosis-inducing factor (AIF) translocates from mitochondria to the parental macronucleus (MAC) in Tetrahymena thermophila. In the degenerating parental MAC, AIF induces chromatin condensation and large-scale DNA fragmentation in a caspase-independent manner. However, the regulation of AIF nuclear translocation and molecular mechanism of PND are less clear. Read More

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http://dx.doi.org/10.1111/febs.14761DOI Listing
January 2019
1 Read
4.001 Impact Factor

Selectivity of the CUBAN domain in the recognition of ubiquitin and NEDD8.

FEBS J 2019 Feb 5;286(4):653-677. Epub 2019 Feb 5.

Department of Biology, Tor Vergata University, Rome, Italy.

Among the members of the ubiquitin-like (Ubl) protein family, neural precursor cell expressed developmentally down-regulated protein 8 (NEDD8) is the closest in sequence to ubiquitin (57% identity). The two modification mechanisms and their functions, however, are highly distinct and the two Ubls are not interchangeable. A complex network of interactions between modifying enzymes and adaptors, most of which are specific while others are promiscuous, ensures selectivity. Read More

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http://dx.doi.org/10.1111/febs.14752DOI Listing
February 2019
1 Read

Metabolic reprogramming in breast cancer results in distinct mitochondrial bioenergetics between luminal and basal subtypes.

FEBS J 2019 Feb 5;286(4):688-709. Epub 2019 Feb 5.

Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy.

Mitochondrial dysfunction is a key feature of cancer and is frequently associated with increased aggressiveness and metastatic potential. Recent evidence has brought to light a metabolic rewiring that takes place during the epithelial-to-mesenchymal transition (EMT), a process that drives the invasive capability of malignant tumors, and highlights a mechanistic link between mitochondrial dysfunction and EMT that has been only partially investigated. In this study, we characterized mitochondrial function and bioenergetic status of cultured human breast cancer cell lines, including luminal-like and basal-like subtypes. Read More

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http://doi.wiley.com/10.1111/febs.14756
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http://dx.doi.org/10.1111/febs.14756DOI Listing
February 2019
4 Reads

Tripping the light fantastic in membrane redox biology: linking dynamic structures to function in ER electron transfer chains.

FEBS J 2019 Jan 18. Epub 2019 Jan 18.

Manchester Institute of Biotechnology and School of Chemistry, University of Manchester, UK.

How the dynamics of proteins assist catalysis is a contemporary issue in enzymology. In particular, this holds true for membrane-bound enzymes, where multiple structural, spectroscopic and biochemical approaches are needed to build up a comprehensive picture of how dynamics influence enzyme reaction cycles. Of note are the recent studies of cytochrome P450 reductases (CPR)-P450 (CYP) endoplasmic reticulum redox chains, showing the relationship between dynamics and electron flow through flavin and haem redox centres and the impact this has on monooxygenation chemistry. Read More

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http://doi.wiley.com/10.1111/febs.14757
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http://dx.doi.org/10.1111/febs.14757DOI Listing
January 2019
5 Reads

DLIC1, but not DLIC2, is upregulated in colon cancer and this contributes to proliferative overgrowth and migratory characteristics of cancer cells.

FEBS J 2019 Feb 7;286(4):803-820. Epub 2019 Feb 7.

Division of Vascular Oncology and Metastasis, German Cancer Research Center, DKFZ-ZMBH Alliance, Heidelberg, Germany.

Cytoplasmic dynein-1 is a large minus-end-directed microtubule motor complex involved in membrane trafficking, organelle positioning, and microtubule organization. The roles of dynein light intermediate chains (DLICs; DLIC1 and DLIC2) within the complex are, however, still largely undefined. In this study, we investigated the possible roles of DLICs in epithelial homeostasis and colon cancer development. Read More

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http://dx.doi.org/10.1111/febs.14755DOI Listing
February 2019
5 Reads

Pressure-induced activation of latent dihydroorotase from Aquifex aeolicus as revealed by high pressure protein crystallography.

FEBS J 2019 Jan 18. Epub 2019 Jan 18.

Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, MI, USA.

Dihydroorotase (DHOase) is involved in the de novo synthesis of pyrimidine in virtually all organisms, and it is usually associated with two other enzymes found in this biosynthetic pathway, carbamylphosphate synthetase and/or aspartate transcarbamylase (ATCase). In the hyperthermophilic bacterium Aquifex aeolicus, ATCase and DHOase are noncovalently associated. Upon dissociation, ATCase keeps its activity entirely while DHOase is totally inactivated. Read More

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http://dx.doi.org/10.1111/febs.14758DOI Listing
January 2019
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Mechanistic insights into enzymatic catalysis by trehalase from the insect gut endosymbiont Enterobacter cloacae.

FEBS J 2019 Jan 18. Epub 2019 Jan 18.

Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, India.

Energy metabolism in the diamondback moth Plutella xylostella is facilitated by trehalase, an enzyme which assists in trehalose hydrolysis, from the predominant gut bacterium Enterobacter cloacae. We report the biochemical and structural characterization of recombinant trehalase from E. cloacae (Px_EclTre). Read More

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http://dx.doi.org/10.1111/febs.14760DOI Listing
January 2019
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Mammalian sugar-binding receptors: known functions and unexplored roles.

FEBS J 2019 Jan 18. Epub 2019 Jan 18.

Department of Life Sciences, Imperial College, London, UK.

Mammalian glycan-binding receptors, sometimes known as lectins, interact with glycans, the oligosaccharide portions of endogenous mammalian glycoproteins and glycolipids as well as sugars on the surfaces of microbes. These receptors guide glycoproteins out of and back into cells, facilitate communication between cells through both adhesion and signaling, and allow the innate immune system to respond quickly to viral, fungal, bacterial, and parasitic pathogens. For many of the roughly 100 glycan-binding receptors that are known in humans, there are good descriptions of what types of glycans they bind and how selectivity for these ligands is achieved at the molecular level. Read More

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http://doi.wiley.com/10.1111/febs.14759
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http://dx.doi.org/10.1111/febs.14759DOI Listing
January 2019
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Diminished membrane recruitment of Akt is instrumental in alcohol-associated osteopenia via the PTEN/Akt/GSK-3β/β-catenin axis.

FEBS J 2019 Jan 17. Epub 2019 Jan 17.

Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Alcohol is considered a leading risk factor for osteopenia. Our previous research indicated that the Akt/GSK-3β/β-catenin pathway plays a critical role in the ethanol-induced antiosteogenic effect in bone mesenchymal stem cells (BMSCs). PI3K/Akt is negatively regulated by the phosphatase and tensin homolog (PTEN) phosphatase. Read More

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http://doi.wiley.com/10.1111/febs.14754
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http://dx.doi.org/10.1111/febs.14754DOI Listing
January 2019
8 Reads
4.001 Impact Factor

NOX1/NADPH oxidase regulates the expression of multidrug resistance-associated protein 1 and maintains intracellular glutathione levels.

FEBS J 2019 Feb 6;286(4):678-687. Epub 2019 Feb 6.

Department of Pharmacology, Kyoto Prefectural University of Medicine, Japan.

The involvement of superoxide-generating NADPH oxidase (NOX) in the cytotoxic effects of cigarette smoke extracts has been documented. However, the underlying molecular mechanisms and NOX isoform involved have not been fully clarified. Among the different NADPH oxidase isoforms identified so far, NOX1 and NOX4 were found to be expressed in rat H9c2 cardiomyocytes. Read More

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http://dx.doi.org/10.1111/febs.14753DOI Listing
February 2019
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The dual role of the glycosaminoglycan chondroitin-6-sulfate in the development, progression and metastasis of cancer.

FEBS J 2019 Jan 13. Epub 2019 Jan 13.

Department of Clinical Chemistry and Laboratory Diagnostics, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Katowice, Poland.

The remarkable structural heterogeneity of chondroitin sulfate (CS) and dermatan sulfate (DS) generates biological information that can be unique to each of these glycosaminoglycans (GAGs), and changes in their composition are translated into alterations in the binding profiles of these molecules. CS/DS can bind to various cytokines and growth factors, cell surface receptors, adhesion molecules, enzymes and fibrillar glycoproteins of the extracellular matrix, thereby influencing both cell behavior and the biomechanical and biochemical properties of the matrix. In this review, we summarize the current knowledge concerning CS/DS metabolism in the human cancer stroma. Read More

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http://doi.wiley.com/10.1111/febs.14748
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http://dx.doi.org/10.1111/febs.14748DOI Listing
January 2019
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The RUNX1-ETO fusion protein trans-activates c-KIT expression by recruiting histone acetyltransferase P300 on its promoter.

FEBS J 2019 Jan 13. Epub 2019 Jan 13.

Department of Hematology, Chinese PLA General Hospital, Beijing, China.

The oncoprotein RUNX1-ETO is the fusion product of t(8;21)(q22;q22) and constitutes one of the most common genetic alterations in acute myeloid leukemia (AML). Abnormal c-KIT overexpression is considered an independent negative prognostic factor for relapse and survival in t(8;21) AML patients. However, the molecular mechanism of high c-KIT expression in t(8;21) AML remains unknown. Read More

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http://doi.wiley.com/10.1111/febs.14751
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http://dx.doi.org/10.1111/febs.14751DOI Listing
January 2019
8 Reads
4.001 Impact Factor

Structural and functional characterization of a frataxin from a thermophilic organism.

FEBS J 2019 Feb 30;286(3):495-506. Epub 2019 Jan 30.

King's College London, UK.

Frataxins form an interesting family of iron-binding proteins with an almost unique fold and are highly conserved from bacteria to primates. They have a pivotal role in iron-sulfur cluster biogenesis as regulators of the rates of cluster formation, as it is testified by the fact that frataxin absence is incompatible with life and reduced levels of the protein lead to the recessive neurodegenerative disease Friedreich's ataxia. Despite its importance, the structure of frataxin has been solved only from relatively few species. Read More

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http://doi.wiley.com/10.1111/febs.14750
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http://dx.doi.org/10.1111/febs.14750DOI Listing
February 2019
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ITGB4 deficiency induces senescence of airway epithelial cells through p53 activation.

FEBS J 2019 Jan 12. Epub 2019 Jan 12.

Department of Physiology, Xiangya School of Medicine, Central South University, Changsha, China.

Aging is characterized by a progressive loss of physiological integrity, leading to impaired organ function and, ultimately, increased vulnerability to death. Many complex diseases are related to aging, including asthma. In the lung, the airway epithelium serves as the first barrier to prevent the access of inspired external stimuli and dictates the initial stress responses. Read More

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http://doi.wiley.com/10.1111/febs.14749
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http://dx.doi.org/10.1111/febs.14749DOI Listing
January 2019
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The ammonium transporter AmtB and the PII signal transduction protein GlnZ are required to inhibit DraG in Azospirillum brasilense.

FEBS J 2019 Jan 11. Epub 2019 Jan 11.

Instituto Nacional de Ciência e Tecnologia da Fixação Biológica de Nitrogênio, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Brazil.

The ammonium-dependent posttranslational regulation of nitrogenase activity in Azospirillum brasilense requires dinitrogenase reductase ADP-ribosyl transferase (DraT) and dinitrogenase reductase ADP-glycohydrolase (DraG). These enzymes are reciprocally regulated by interaction with the PII proteins, GlnB and GlnZ. In this study, purified ADP-ribosylated Fe-protein was used as substrate to study the mechanism involved in the regulation of A. Read More

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http://dx.doi.org/10.1111/febs.14745DOI Listing
January 2019
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Molecular insights into the mechanism of 4-hydroxyphenylpyruvate dioxygenase inhibition: enzyme kinetics, X-ray crystallography and computational simulations.

FEBS J 2019 Jan 11. Epub 2019 Jan 11.

Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, International Joint Research Center for Intelligent Biosensor Technology and Health, Chemical Biology Center, College of Chemistry, Central China Normal University, Wuhan, China.

Slow-binding inhibitors with long residence time on the target often display superior efficacy in vivo. Rationally designing inhibitors with low off-target rates is restricted by a limited understanding of the structural basis of slow-binding inhibition kinetics in enzyme-drug interactions. 4-Hydroxyphenylpyruvate dioxygenase (HPPD) is an important target for drug and herbicide development. Read More

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http://doi.wiley.com/10.1111/febs.14747
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http://dx.doi.org/10.1111/febs.14747DOI Listing
January 2019
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How to prepare and deliver a great talk.

FEBS J 2019 Jan;286(1):39-45

The FEBS Journal Editorial Office, Cambridge, UK.

Giving effective and interesting talks are key skills for scientists at every stage of their career. This installment of the Words of Advice series provides some insights into preparing and delivering compelling presentations, as well as alleviating pretalk nerves. Read More

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http://dx.doi.org/10.1111/febs.14726DOI Listing
January 2019
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