11,877 results match your criteria FASEB journal : official publication of the Federation of American Societies for Experimental Biology[Journal]


Loss of growth hormone-mediated signal transducer and activator of transcription 5 (STAT5) signaling in mice results in insulin sensitivity with obesity.

FASEB J 2019 Feb 19:fj201802328R. Epub 2019 Feb 19.

Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia.

Growth hormone (GH) has an important function as an insulin antagonist with elevated insulin sensitivity evident in humans and mice lacking a functional GH receptor (GHR). We sought the molecular basis for this sensitivity by utilizing a panel of mice possessing specific deletions of GHR signaling pathways. Metabolic clamps and glucose homeostasis tests were undertaken in these obese adult C57BL/6 male mice, which indicated impaired hepatic gluconeogenesis. Read More

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http://dx.doi.org/10.1096/fj.201802328RDOI Listing
February 2019

Unravelling the transcriptional responses of TGF-β: Smad3 and EZH2 constitute a regulatory switch that controls neuroretinal epithelial cell fate specification.

FASEB J 2019 Feb 19:fj201800566RR. Epub 2019 Feb 19.

Diabetes Complications Research Centre, Conway Institute of Biomolecular and Biomedical Science, University College Dublin, Dublin, Ireland.

Cell differentiation is directed by extracellular cues and intrinsic epigenetic modifications, which control chromatin organization and transcriptional activation. Central to this process is PRC2, which modulates the di- and trimethylation of lysine 27 on histone 3; however, little is known concerning the direction of PRC2 to specific loci. Here, we have investigated the physical interactome of EZH2, the enzymatic core of PRC2, during retinoic acid-mediated differentiation of neuroepithelial, pluripotent NT2 cells and the dedifferentiation of neuroretinal epithelial ARPE19 cells in response to TGF-β. Read More

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http://dx.doi.org/10.1096/fj.201800566RRDOI Listing
February 2019

Ceria nanoparticles enhance endochondral ossification-based critical-sized bone defect regeneration by promoting the hypertrophic differentiation of BMSCs via DHX15 activation.

FASEB J 2019 Feb 18:fj201802187R. Epub 2019 Feb 18.

Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China.

Central ischemic necrosis is one of the biggest obstacles in the clinical application of traditional tissue-engineered bone (TEB) in critical-sized bone defect regeneration. Because of its ability to promote vascular invasion, endochondral ossification-based TEB has been applied for bone defect regeneration. However, inadequate chondrocyte hypertrophy can hinder vascular invasion and matrix mineralization during endochondral ossification. Read More

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http://dx.doi.org/10.1096/fj.201802187RDOI Listing
February 2019
5.043 Impact Factor

ATP redirects cytokine trafficking and promotes novel membrane TNF signaling via microvesicles.

FASEB J 2019 Feb 18:fj201802386R. Epub 2019 Feb 18.

Section of Anaesthetics, Pain Medicine, and Intensive Care, Faculty of Medicine, Chelsea and Westminster Hospital, Imperial College London, London, United Kingdom.

Cellular stress or injury induces release of endogenous danger signals such as ATP, which plays a central role in activating immune cells. ATP is essential for the release of nonclassically secreted cytokines such as IL-1β but, paradoxically, has been reported to inhibit the release of classically secreted cytokines such as TNF. Here, we reveal that ATP does switch off soluble TNF (17 kDa) release from LPS-treated macrophages, but rather than inhibiting the entire TNF secretion, ATP packages membrane TNF (26 kDa) within microvesicles (MVs). Read More

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http://dx.doi.org/10.1096/fj.201802386RDOI Listing
February 2019

Trophoblastic proliferation and invasion regulated by ACTN4 is impaired in early onset preeclampsia.

FASEB J 2019 Feb 18:fj201802058RR. Epub 2019 Feb 18.

State Key Laboratory of Maternal and Fetal Medicine of Chongqing Municipality, Chongqing Medical University, Chongqing, China.

Successful pregnancy requires normal placentation, which largely depends on the tight regulation of proliferation, invasion, and migration of trophoblast cells. Abnormal functioning of trophoblast cells may cause failure of uterine spiral artery remodeling, which may be related to pregnancy-related disorders, such as preeclampsia. Here, we reported that an actin-binding protein, α-actinin (ACTN)4, was dysregulated in placentas from early onset preeclampsia. Read More

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http://dx.doi.org/10.1096/fj.201802058RRDOI Listing
February 2019

Transactivation domain of Krüppel-like factor 15 negatively regulates angiotensin II-induced adventitial inflammation and fibrosis.

FASEB J 2019 Feb 18:fj201801809R. Epub 2019 Feb 18.

Department of Hypertension, State Key Laboratory of Medical Genomics, Shanghai Key Laboratory of Hypertension, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Krüppel-like factor (KLF) 15 has emerged as a critical regulator of fibrosis in cardiovascular diseases. However, the precise role that KLF15 and its functional domain played in adventitial inflammation and fibrosis remains unclear. This study aims to investigate the role of the transactivation domain (TAD) of KLF15 in angiotensin II (Ang II)-induced adventitial pathologic changes. Read More

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http://dx.doi.org/10.1096/fj.201801809RDOI Listing
February 2019

Aquaporin-3 deficiency slows cyst enlargement in experimental mouse models of autosomal dominant polycystic kidney disease.

FASEB J 2019 Feb 15:fj201801338RRR. Epub 2019 Feb 15.

Department of Pharmacology, School of Basic Medical Sciences, Peking University, Beijing, China.

Human autosomal dominant polycystic kidney disease (ADPKD) is characterized by bilateral renal cysts that lead to a decline in kidney function. Previous studies reported aquaporin (AQP)-3 expression in cysts derived from collecting ducts in ADPKD. To study the role of AQP3 in cyst development, we generated 2 polycystic kidney disease (PKD) mouse models: kidney-specific Pkd1 knockout mice and inducible Pkd1 knockout mice, each without and with AQP3 deletion. Read More

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http://dx.doi.org/10.1096/fj.201801338RRRDOI Listing
February 2019

Talin promotes integrin activation accompanied by generation of tension in talin and an increase in osmotic pressure in neurite outgrowth.

FASEB J 2019 Feb 15:fj201801949RR. Epub 2019 Feb 15.

State Key Laboratory Cultivation Base for Traditional Chinese Medicine (TCM) Quality and Efficacy, School of Medicine and Life Science, Nanjing University of Chinese Medicine, Nanjing, China.

Neuronal polarization depends on the interaction of intracellular chemical and mechanical activities in which the cytoplasmic protein, talin, plays a pivotal role during neurite growth. To better understand the mechanism underlying talin function in neuronal polarization, we overexpressed several truncated forms of talin and found that the presence of the rod domain within the overexpressed talin is required for its positive effect on neurite elongation because the neurite number only increased when the talin head region was overexpressed. The tension in the talin rod was recognized using a Förster resonance energy transfer-based tension probe. Read More

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http://dx.doi.org/10.1096/fj.201801949RRDOI Listing
February 2019

Paternal high-fat diet transgenerationally impacts hepatic immunometabolism.

FASEB J 2019 Feb 15:fj201801879RR. Epub 2019 Feb 15.

Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.

Paternal preconceptional high-fat diet (HFD) alters whole-body glucose and energy homeostasis over several generations, which may be mediated by altered transcriptomic profiles of metabolic organs. We investigated the effect of paternal HFD on the hepatic transcriptomic and metabolic signatures of female grand-offspring. Paternal HFD strongly impacted the liver transcriptome of the second-generation offspring. Read More

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http://dx.doi.org/10.1096/fj.201801879RRDOI Listing
February 2019

iPSC- and mesenchymoangioblast-derived mesenchymal stem cells provide greater protection against experimental chronic allergic airways disease compared with a clinically used corticosteroid.

FASEB J 2019 Feb 15:fj201802307R. Epub 2019 Feb 15.

Monash Biomedicine Discovery Institute Monash University, Clayton, Victoria, Australia.

The airway remodeling (AWR) associated with chronic allergic airways disease (AAD)/asthma contributes to irreversible airway obstruction. This study compared and combined the antiremodeling and other effects of induced pluripotent stem cell and mesenchymoangioblast-derived mesenchymal stem cells (MCA-MSCs) with the corticosteroid dexamethasone (Dex) in experimental chronic AAD/asthma. Female BALB/c mice subjected to 11 wk of ovalbumin (Ova)-induced chronic AAD were intranasally administered MCA-MSCs (1 × 10 cells/mouse; once weekly on wk 10 and 11), Dex (0. Read More

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http://dx.doi.org/10.1096/fj.201802307RDOI Listing
February 2019

Obesogenic diet in aging mice disrupts gut microbe composition and alters neutrophil:lymphocyte ratio, leading to inflamed milieu in acute heart failure.

FASEB J 2019 Feb 15:fj201802477R. Epub 2019 Feb 15.

Division of Cardiovascular Disease, Department of Medicine, The University of Alabama at Birmingham, Birmingham, Alabama, USA.

Calorie-dense obesogenic diet (OBD) is a prime risk factor for cardiovascular disease in aging. However, increasing age coupled with changes in the diet can affect the interaction of intestinal microbiota influencing the immune system, which can lead to chronic inflammation. How age and calorie-enriched OBD interact with microbial flora and impact leukocyte profiling is currently under investigated. Read More

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http://dx.doi.org/10.1096/fj.201802477RDOI Listing
February 2019

Taurine alleviates repression of betaine-homocysteine S-methyltransferase and significantly improves the efficacy of long-term betaine treatment in a mouse model of cystathionine β-synthase-deficient homocystinuria.

FASEB J 2019 Feb 15:fj201802069RR. Epub 2019 Feb 15.

Department of Medicine, University of Colorado School of Medicine, Aurora, Colorado, USA.

Classical cystathionine β-synthase-deficient homocystinuria (HCU) is a life-threatening inborn error of sulfur metabolism. Treatment for pyridoxine-nonresponsive HCU involves lowering homocysteine (Hcy) with a methionine (Met)-restricted diet and betaine supplementation. Betaine treatment efficacy diminishes significantly over time due to impairment of betaine-Hcy S-methyltransferase (BHMT) function. Read More

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http://dx.doi.org/10.1096/fj.201802069RRDOI Listing
February 2019

RCC2, a regulator of the RalA signaling pathway, is identified as a novel therapeutic target in cisplatin-resistant ovarian cancer.

FASEB J 2019 Feb 15:fj201801529RR. Epub 2019 Feb 15.

Department of Gynecology, People's Hospital of Huadu District, Guangzhou, China.

Currently, cisplatin (DDP) is the first-line chemotherapeutic agent used for treatment of ovarian cancer, but gradually acquired drug resistance minimizes its therapeutic outcomes. We aimed to identify crucial genes associated with DDP resistance in ovarian cancer and uncover potential mechanisms. Two sets of gene expression data were downloaded from Gene Expression Omnibus, and bioinformatics analysis was conducted. Read More

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http://dx.doi.org/10.1096/fj.201801529RRDOI Listing
February 2019

Enhanced osteogenic differentiation of human bone-derived mesenchymal stem cells in 3-dimensional printed porous titanium scaffolds by static magnetic field through up-regulating Smad4.

FASEB J 2019 Feb 14:fj201802195R. Epub 2019 Feb 14.

Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Peking Union Medical College-Chinese Academy of Medical Sciences, Beijing, China.

The reconstruction of large bone defects remains a significant challenge for orthopedists. Three-dimensional-printed (3DP) scaffold is considered a promising repair material. Static magnetic field (SMF) treatment is an effective and noninvasive therapeutic method to improve bone regeneration. Read More

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http://dx.doi.org/10.1096/fj.201802195RDOI Listing
February 2019
5.043 Impact Factor

A follistatin-based molecule increases muscle and bone mass without affecting the red blood cell count in mice.

FASEB J 2019 Feb 13:fj201801969RR. Epub 2019 Feb 13.

Department of Renal Medicine, Aarhus University Hospital, Aarhus, Denmark.

Inhibitors of the activin receptor signaling pathway (IASPs) have become candidate therapeutics for sarcopenia and bone remodeling disorders because of their ability to increase muscle and bone mass. However, IASPs utilizing activin type IIA and IIB receptors are also potent stimulators of erythropoiesis, a feature that may restrict their usage to anemic patients because of increased risk of venous thromboembolism. Based on the endogenous TGF-β superfamily antagonist follistatin (FST), a molecule in the IASP class, FST-mFc, was generated and tested in both ovariectomized and naive BALB/c and C57BL/6 mice. Read More

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http://dx.doi.org/10.1096/fj.201801969RRDOI Listing
February 2019
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Functional definition of NrtR, a remnant regulator of NAD homeostasis in the zoonotic pathogen Streptococcus suis.

FASEB J 2019 Feb 13:fj201802179RR. Epub 2019 Feb 13.

Department of Pathogen Biology & Microbiology, Department of General Intensive Care Unit of Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

NAD is an enzyme cofactor required for the 3 domains of life. However, little is known about the NAD biosynthesis and salvage pathways in the opportunistic pathogen Streptococcus suis. A genome-wide search allows us to identify the NAD salvage pathway encoded by an operon of nadR-pnuC-nrtR (from SSU05_1973 to SSU05_1971 on the reverse strand) in the S. Read More

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http://dx.doi.org/10.1096/fj.201802179RRDOI Listing
February 2019

Intrauterine exposure to hyperglycemia retards the development of brown adipose tissue.

FASEB J 2019 Feb 13:fj201801818R. Epub 2019 Feb 13.

Key Laboratory of Reproductive Genetics, Ministry of Education, Zhejiang University, Hangzhou, China.

Brown adipose tissue (BAT) is an exclusive tissue of nonshivering thermogenesis. It is fueled by lipids and glucose and involved in energy and metabolic homeostasis. Intrauterine exposure to hyperglycemia during gestational diabetes mellitus may result in abnormal fetal development and metabolic phenotypes in adulthood. Read More

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http://dx.doi.org/10.1096/fj.201801818RDOI Listing
February 2019

AMPK-mediated degradation of Nav1.5 through autophagy.

FASEB J 2019 Feb 13:fj201801583RR. Epub 2019 Feb 13.

Department of Cardiology, Drum Tower Hospital, Nanjing University Medical School, Nanjing, China.

The voltage-gated cardiac sodium channel, Nav1.5, is the key component that controls cardiac excitative electrical impulse and propagation. However, the dynamic alterations of Nav1. Read More

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http://dx.doi.org/10.1096/fj.201801583RRDOI Listing
February 2019

Toll-like receptor 3 controls QT interval on the electrocardiogram by targeting the degradation of Kv4.2/4.3 channels in the endoplasmic reticulum.

FASEB J 2019 Feb 13:fj201801464R. Epub 2019 Feb 13.

Heart Health Center, East Hospital, Tongji University School of Medicine, Shanghai, China.

TLRs have been proven to be essential mediators for the early innate immune response. Overactivation of TLR-mediated immune signaling promotes deterioration of cardiovascular diseases; however, the role of TLRs in the heart under physiologic conditions remains neglected. Here, we show that Tlr3 deficiency induced the endoplasmic reticulum (ER) retention of Kv4. Read More

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http://dx.doi.org/10.1096/fj.201801464RDOI Listing
February 2019

Betaine-homocysteine S-methyltransferase deficiency causes increased susceptibility to noise-induced hearing loss associated to plasma hyperhomocysteinemia.

FASEB J 2019 Feb 12:fj201801533R. Epub 2019 Feb 12.

Instituto de Investigaciones Biomédicas Alberto Sols, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), Madrid, Spain.

Betaine-homocysteine S-methyltransferases (BHMTs) are methionine cycle enzymes that remethylate homocysteine; hence, their malfunction leads to hyperhomocysteinemia. Epidemiologic and experimental studies have revealed a correlation between hyperhomocysteinemia and hearing loss. Here, we have studied the expression of methionine cycle genes in the mouse cochlea and the impact of knocking out the Bhmt gene in the auditory receptor. Read More

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http://dx.doi.org/10.1096/fj.201801533RDOI Listing
February 2019
5.043 Impact Factor

Vaccine efficacy of a Mycobacterium tuberculosis Beijing-specific proline-glutamic acid (PE) antigen against highly virulent outbreak isolates.

FASEB J 2019 Feb 12:fj201802604R. Epub 2019 Feb 12.

Department of Microbiology, Institute for Immunology and Immunological Disease, Brain Korea 21 Program for Leading Universities and Students (PLUS) Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea.

Bacillus Calmette-Guerin vaccine confers insufficient pulmonary protection against tuberculosis (TB), particularly the Mycobacterium tuberculosis (Mtb) Beijing strain infection. Identification of vaccine antigens (Ags) by considering Mtb genetic diversity is crucial for the development of improved TB vaccine. MTBK_20640, a new Beijing genotype-specific proline-glutamic acid-family Ag, was identified by comparative genomic analysis. Read More

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http://dx.doi.org/10.1096/fj.201802604RDOI Listing
February 2019
1 Read

Expression of miRNAs in circulating exosomes derived from patients with persistent atrial fibrillation.

FASEB J 2019 Feb 12:fj201801758R. Epub 2019 Feb 12.

Division of Cardiology, Yonsei University College of Medicine, Seoul, South Korea.

Atrial fibrillation (AF), the most common type of cardiac arrhythmia, is thought to be regulated by changes in microRNA (miRNA) expression. However, the evidence for this is inconsistent. The high stability and expression of circulating exosomal miRNAs may allow their use as candidate biomarkers. Read More

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http://dx.doi.org/10.1096/fj.201801758RDOI Listing
February 2019
1 Read

Regulation and role of endoglin in cholesterol-induced endothelial and vascular dysfunction in vivo and in vitro.

FASEB J 2019 Feb 12:fj201802245R. Epub 2019 Feb 12.

Department of Biological and Medical Sciences, Faculty of Pharmacy in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic.

Our objective was to investigate the effect of cholesterol [hypercholesterolemia and 7-ketocholesterol (7K)] on endoglin (Eng) expression and regulation with respect to endothelial or vascular dysfunction in vivo and in vitro. In vivo experiments were performed in 2-mo-old atherosclerosis-prone apolipoprotein E-deficient/LDL receptor-deficient (ApoE/LDLR) female mice and their wild-type C57BL/6J littermates. In in vitro experiments, human aortic endothelial cells (HAECs) were treated with 7K. Read More

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http://dx.doi.org/10.1096/fj.201802245RDOI Listing
February 2019

Neuroprotective effect of placenta-derived mesenchymal stromal cells: role of exosomes.

FASEB J 2019 Feb 12:fj201800972R. Epub 2019 Feb 12.

Surgical Bioengineering Laboratory, Department of Surgery, University of California-Davis, Sacramento, California, USA.

We have established early-gestation chorionic villus-derived placenta mesenchymal stromal cells (PMSCs) as a potential treatment for spina bifida (SB), a neural tube defect. Our preclinical studies demonstrated that PMSCs have the potential to cure hind limb paralysis in the fetal lamb model of SB via a paracrine mechanism. PMSCs exhibit neuroprotective function by increasing cell number and neurites, as shown by indirect coculture and direct addition of PMSC-conditioned media to the staurosporine-induced apoptotic human neuroblastoma cell line, SH-SY5Y. Read More

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http://dx.doi.org/10.1096/fj.201800972RDOI Listing
February 2019
2 Reads

Neuromedin U is a gut peptide that alters oral glucose tolerance by delaying gastric emptying via direct contraction of the pylorus and vagal-dependent mechanisms.

FASEB J 2019 Feb 12:fj201801718R. Epub 2019 Feb 12.

Institut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche (UMR) 1149, Assistance Publique-Hôpitaux de Paris (AP-HP), Université Paris Diderot, Paris, France.

The gut-brain peptide neuromedin U (NMU) decreases food intake and body weight and improves glucose tolerance. Here, we characterized NMU as an enteropeptide and determined how it impacts glucose excursion. NMU was expressed predominantly in the proximal small intestine, and its secretion was triggered by ingestion of a mixed meal. Read More

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http://dx.doi.org/10.1096/fj.201801718RDOI Listing
February 2019

A miR-29a-driven negative feedback loop regulates peripheral glucocorticoid receptor signaling.

FASEB J 2019 Feb 11:fj201801385RR. Epub 2019 Feb 11.

Institute for Diabetes and Cancer (IDC), Helmholtz Center Munich, Neuherberg, Germany.

The glucocorticoid receptor (GR) represents the crucial molecular mediator of key endocrine, glucocorticoid hormone-dependent regulatory circuits, including control of glucose, protein, and lipid homeostasis. Consequently, aberrant glucocorticoid signaling is linked to severe metabolic disorders, including insulin resistance, obesity, and hyperglycemia, all of which also appear upon chronic glucocorticoid therapy for the treatment of inflammatory conditions. Of note, long-term glucocorticoid exposure under these therapeutic conditions typically induces glucocorticoid resistance, requiring higher doses and consequently triggering more severe metabolic phenotypes. Read More

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http://dx.doi.org/10.1096/fj.201801385RRDOI Listing
February 2019
1 Read

TAZ stimulates liver regeneration through interleukin-6-induced hepatocyte proliferation and inhibition of cell death after liver injury.

FASEB J 2019 Feb 11:fj201801256RR. Epub 2019 Feb 11.

Department of Life Sciences, School of Life Sciences and Biotechnology, Korea University, Seoul, South Korea.

In response to liver injury, the liver undergoes a regeneration process to retain its mass and function. However, the regeneration mechanism has not been fully clarified. This study investigated the role of transcriptional coactivator with PDZ-binding motif (TAZ), a Hippo-signaling effector, in liver regeneration. Read More

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http://dx.doi.org/10.1096/fj.201801256RRDOI Listing
February 2019

Synaptosomal-associated protein 29 is required for the autophagic degradation of hepatitis B virus.

FASEB J 2019 Feb 11:fj201801995RR. Epub 2019 Feb 11.

Institute of Virology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.

Hepatitis B virus (HBV) replication and envelopment is dependent on cellular autophagy. Previously, we have provided evidence for the extensive lysosomal degradation of HBV virions and the hepatitis B surface antigen (HBsAg), which is likely controlled by autophagosome-lysosome fusion. Synaptosomal-associated protein 29 (SNAP29) has been identified as a protein specifically mediating autophagosome-lysosome fusion. Read More

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http://dx.doi.org/10.1096/fj.201801995RRDOI Listing
February 2019
1 Read

Astrocytic cystine/glutamate antiporter is a key regulator of erythropoietin expression in the ischemic retina.

FASEB J 2019 Feb 11:fj201802144R. Epub 2019 Feb 11.

Fight Against Angiogenesis-Related Blindness (FARB) Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul National University, Seoul, South Korea.

Ischemic retinopathies and optic neuropathies are important causes of vision loss. The neuroprotective effect of erythropoietin (EPO) in ischemic neuronal injury and the expression of EPO and its receptor in retinal tissue have been well documented. However, the exact regulatory mechanism of EPO expression in retinal ischemia still remains to be elucidated. Read More

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http://dx.doi.org/10.1096/fj.201802144RDOI Listing
February 2019

Functional interactions between scaffold proteins, noncoding RNAs, and genome loci induce liquid-liquid phase separation as organizing principle for 3-dimensional nuclear architecture: implications in cancer.

FASEB J 2019 Feb 11:fj201802715R. Epub 2019 Feb 11.

Lung Cancer Epigenetic, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.

The eukaryotic cell nucleus consists of functionally specialized subcompartments. These nuclear subcompartments are biomolecular aggregates built of proteins, transcripts, and specific genome loci. The structure and function of each nuclear subcompartment are defined by the composition and dynamic interaction between these 3 components. Read More

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http://dx.doi.org/10.1096/fj.201802715RDOI Listing
February 2019

Yes-associated protein promotes early hepatocyte cell cycle progression in regenerating liver after tissue loss.

FASEB Bioadv 2019 Jan 27;1(1):51-61. Epub 2018 Nov 27.

Laboratory of the Swiss HPB and Transplantation Center, Department of Surgery University Hospital Zürich Zürich Switzerland.

The ability of the liver to restore its original volume following tissue loss has been associated with the Hippo-YAP1 pathway, a key controller of organ size. Yes-associated protein 1 (YAP1)-a growth effector usually restrained by Hippo signaling-is believed to be of particular importance; however, its role in liver regeneration remains ill-defined. To explore its function, we knocked down YAP1 prior to standard 70%-hepatectomy (sHx) using a hepatocyte-specific nanoformulation. Read More

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http://dx.doi.org/10.1096/fba.1023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351850PMC
January 2019
1 Read

A comparison of unamplified and massively multiplexed PCR amplification for murine antibody repertoire sequencing.

FASEB Bioadv 2019 Jan 30;1(1):6-17. Epub 2018 Aug 30.

Division of Biology, Kansas State University, Manhattan, KS, USA.

Sequencing antibody repertoires has steadily become cheaper and easier. Sequencing methods usually rely on some form of amplification, often a massively multiplexed PCR prior to sequencing. To eliminate potential biases and create a data set that could be used for other studies, our lab compared unamplified sequencing results from the splenic heavy-chain repertoire in the mouse to those processed through two commercial applications. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366624PMC
January 2019

Prolactin regulation of insulin-like growth factor 2 gene expression in the adult mouse choroid plexus.

FASEB J 2019 Feb 8:fj201802262R. Epub 2019 Feb 8.

Centre for Neuroendocrinology, School of Medical Sciences, University of Otago, Dunedin, New Zealand.

There is clear evidence for carrier-mediated transport of prolactin into the brain, and it has been widely assumed that prolactin receptors (PRLRs) in the choroid plexus (ChP) might mediate this transport. Using PRLR knockout mice, we recently showed that PRLRs in ChP are not required for prolactin transport into the brain. Hence, the function of PRLR in the ChP remains unknown. Read More

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http://dx.doi.org/10.1096/fj.201802262RDOI Listing
February 2019

Targeting biosynthetic networks of the proinflammatory and proresolving lipid metabolome.

FASEB J 2019 Feb 8:fj201802509R. Epub 2019 Feb 8.

Department of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich Schiller University Jena, Jena, Germany.

Nonsteroidal anti-inflammatory drugs interfere with the metabolism of arachidonic acid to proinflammatory prostaglandins and leukotrienes by targeting cyclooxygenases (COXs), 5-lipoxygenase (LOX), or the 5-LOX-activating protein (FLAP). These and related enzymes act in conjunction with marked crosstalk within a complex lipid mediator (LM) network where also specialized proresolving LMs (SPMs) are formed. Here, we present how prominent LM pathways can be differentially modulated in human proinflammatory M1 and proresolving M2 macrophage phenotypes that, upon exposure to Escherichia coli, produce either abundant prostaglandins and leukotrienes (M1) or SPMs (M2). Read More

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http://dx.doi.org/10.1096/fj.201802509RDOI Listing
February 2019

CD11bLy6G myeloid-derived suppressor cells promote liver regeneration in a murine model of major hepatectomy.

FASEB J 2019 Feb 7:fj201801733R. Epub 2019 Feb 7.

The Surgical Division, Department of Surgery B, The Laboratory of Molecular Genetics, Hepato-Pancreato-Biliary (HPB) Cancer Research, Tel-Aviv Sourasky Medical Center-The Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel.

Liver regeneration depends on sequential activation of pathways and cells involving the remaining organ in recovery of mass. Proliferation of parenchyma is dependent on angiogenesis. Understanding liver regeneration-associated neovascularization may be useful for development of clinical interventions. Read More

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https://www.fasebj.org/doi/10.1096/fj.201801733R
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http://dx.doi.org/10.1096/fj.201801733RDOI Listing
February 2019
1 Read

Muscle cannabinoid 1 receptor regulates Il-6 and myostatin expression, governing physical performance and whole-body metabolism.

FASEB J 2019 Feb 6:fj201801145R. Epub 2019 Feb 6.

Laboratory of Clinical Investigation, National Institute on Aging, National Institutes of Health (NIH), Baltimore, Maryland, USA.

Sarcopenic obesity, the combination of skeletal muscle mass and function loss with an increase in body fat, is associated with physical limitations, cardiovascular diseases, metabolic stress, and increased risk of mortality. Cannabinoid receptor type 1 (CB1R) plays a critical role in the regulation of whole-body energy metabolism because of its involvement in controlling appetite, fuel distribution, and utilization. Inhibition of CB1R improves insulin secretion and insulin sensitivity in pancreatic β-cells and hepatocytes. Read More

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http://dx.doi.org/10.1096/fj.201801145RDOI Listing
February 2019
1 Read

Myeloid cells require gp130 signaling for protective anti-inflammatory functions during sepsis.

FASEB J 2019 Feb 6:fj201802118R. Epub 2019 Feb 6.

Department of Internal Medicine III, University Hospital Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen, Aachen, Germany.

Sepsis represents a major health problem worldwide because of high mortality rates and cost-intensive therapy. Immunomodulatory strategies as a means of controlling overshooting inflammatory responses during sepsis have thus far not been effective, and there is a general paucity of new therapies. Regulatory immune cells have been shown to play important roles in limiting systemic inflammation. Read More

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http://dx.doi.org/10.1096/fj.201802118RDOI Listing
February 2019

TIGAR regulates mitochondrial functions through SIRT1-PGC1α pathway and translocation of TIGAR into mitochondria in skeletal muscle.

FASEB J 2019 Feb 6:fj201802209R. Epub 2019 Feb 6.

Department of Pharmacology and Laboratory of Aging and Nervous Diseases, Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, School of Pharmaceutical Sciences, Soochow University, Suzhou, China.

TP53-induced glycolysis and apoptosis regulator (TIGAR), a glycolytic inhibitor, plays vital roles in regulating cellular metabolism and oxidative stress. However, the role of highly expressed TIGAR in skeletal muscle remains unexplored. In the present study, TIGAR levels varied in different skeletal muscles and fibers. Read More

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http://dx.doi.org/10.1096/fj.201802209RDOI Listing
February 2019
6 Reads

Cyclin-dependent kinase 9 (CDK9) is a novel prognostic marker and therapeutic target in ovarian cancer.

FASEB J 2019 Feb 6:fj201801789RR. Epub 2019 Feb 6.

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Despite surgical and chemotherapeutic advances over the past few decades, the prognosis for ovarian cancer remains very poor. Although cyclin-dependent kinase (CDK) 9 has an established pathogenic role in various cancers, its function in ovarian cancer remains poorly defined. The purpose of this study was to evaluate the expression of CDK9 and its therapeutic potential in ovarian cancer. Read More

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https://www.fasebj.org/doi/10.1096/fj.201801789RR
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http://dx.doi.org/10.1096/fj.201801789RRDOI Listing
February 2019
6 Reads

IL-6/Smad2 signaling mediates acute kidney injury and regeneration in a murine model of neonatal hyperoxia.

FASEB J 2019 Feb 5:fj201801875RR. Epub 2019 Feb 5.

Translational Experimental Pediatrics, University of Cologne, Faculty of Medicine and University Hospital Cologne, Cologne, Germany.

Prematurity is linked to incomplete nephrogenesis and risk of chronic kidney diseases (CKDs). Oxygen is life-saving in that context but induces injury in numerous organs. Here, we studied the structural and functional impact of hyperoxia on renal injury and its IL-6 dependency. Read More

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February 2019
2 Reads

Spaceflight/microgravity inhibits the proliferation of hematopoietic stem cells by decreasing Kit-Ras/cAMP-CREB pathway networks as evidenced by RNA-Seq assays.

FASEB J 2019 Feb 5:fj201802413R. Epub 2019 Feb 5.

State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Exposure to spaceflight and microgravity causes physiologic and psychologic changes including bone loss, cardiovascular dysfunction, and immune dysfunction. Anemia and hematopoietic disorders are observed in astronauts after spaceflight. Hematopoietic stem and progenitor cells (HSPCs), which can self-renew and give rise to all blood cells, play vital roles in hematopoiesis and homeostasis; however, the molecular mechanisms responsible for the impacts of microgravity on the proliferation of HSPCs remain unclear. Read More

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February 2019

Fndc5 loss-of-function attenuates exercise-induced browning of white adipose tissue in mice.

FASEB J 2019 Feb 5:fj201801754RR. Epub 2019 Feb 5.

Department of Animal Sciences, Purdue University, West Lafayette, Indiana, USA.

Fibronectin type III domain containing 5 (Fndc5) is a transmembrane protein highly expressed in the skeletal muscle. It was reported that exercise promotes the shedding of the extracellular domain of Fndc5, generating a circulating peptide (irisin) that cross-talks to adipose tissues to convert lipid-storing white adipocytes to energy-catabolizing beige adipocytes. However, the requirement of Fndc5 in mediating the beneficial effect of exercise remains to be determined. Read More

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February 2019
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TBC1D3 regulates the payload and biological activity of extracellular vesicles that mediate tissue repair.

FASEB J 2019 Feb 4:fj201802388R. Epub 2019 Feb 4.

Department of Surgery, University of California-San Diego, La Jolla, California, USA.

Healthy repair of cutaneous injury is a coordinated response of inflammatory cells, secreted factors, and biologically active extracellular vesicles (EVs). Although constitutive release of EVs into biologic fluids is a hallmark of cultured cells and tumors, their payload and biologic activity appears to be tightly regulated. We show that Tre-2/Bub2/Cdc16 (TBC1) domain family member 3 (TBC1D3) drives the release of an EV population that causes a decrease in phosphorylation of the transcription factor signal transducer and activator of transcription 3 in naive recipient cells. Read More

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February 2019
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5.043 Impact Factor

Fatty acid type-specific regulation of SIRT1 does not affect insulin sensitivity in human skeletal muscle.

FASEB J 2019 Feb 1:fj201801950R. Epub 2019 Feb 1.

Section of Molecular Physiology, Department of Nutrition, Exercise, and Sports, Faculty of Science, University of Copenhagen, Copenhagen, Denmark.

The nicotinamide adenine dinucleotide-dependent deacetylase, sirtuin (SIRT)1, in skeletal muscle is reduced in insulin-resistant states. However, whether this is an initial mechanism responsible for mediating insulin resistance in human skeletal muscle remains to be investigated. Also, SIRT1 acts as a mitochondrial gene transcriptional regulator and is induced by a short-term, high-fat diet (HFD) in human skeletal muscle. Read More

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February 2019

Modern approaches to investigating non-neuronal aspects of Alzheimer's disease.

Authors:
Shane A Liddelow

FASEB J 2019 Feb;33(2):1528-1535

Neuroscience Institute and Department of Neuroscience and Physiology, New York University Langone Medical Center, New York, New York, USA; and.

The slow, continuous, devastating march of Alzheimer's disease continues to move across the globe. As a society, we are at a loss for options to treat or reverse the death of neurons-the final, apparently inescapable, hallmark of the disease. A continued focus on these dying neurons has taught us much about the disease but with no knowledge-based effective treatment in sight. Read More

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http://dx.doi.org/10.1096/fj.201802592DOI Listing
February 2019

Omega-3 fatty acids, cardiovascular risk, and the resolution of inflammation.

FASEB J 2019 Feb;33(2):1536-1539

Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden; and.

Although a high marine food intake is considered cardioprotective, randomized trials of ω-3 fatty acids initially generated conflicting results in terms of the role of ω-3 supplementation in cardiovascular prevention. This work demonstrates the results of the 3 most recent clinical trials with ω-3 fatty acids are put into the context of possible mechanisms mediating their beneficial cardiovascular effects. In particular, the randomized Reduction of Cardiovascular Events with EPA Intervention Trial (REDUCE-IT) showed that icosapent ethyl, which is the ethyl ester form of the ω-3 fatty acid eicosapentaenoic acid (EPA), induced a significant reduction of cardiovascular events. Read More

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https://www.fasebj.org/doi/10.1096/fj.201802445R
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February 2019
7 Reads

Transgelin 2 is required for embryo implantation by promoting actin polymerization.

FASEB J 2019 Jan 31:fj201802158RRR. Epub 2019 Jan 31.

Department of Obstetrics and Gynecology, Peking University Shenzhen Hospital, Shenzhen, China.

Infertility has been a great challenge in reproductive medicine. At least 40% of human pregnancy losses are clinically unrecognized and occur because of embryo implantation failure. Identification of the proteins and biochemical factors involved in embryo implantation and that are essential for crosstalk between the embryo and uterus can further increase female fertility rates. Read More

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January 2019
3 Reads

Psoralen accelerates bone fracture healing by activating both osteoclasts and osteoblasts.

FASEB J 2019 Jan 31:fj201801797R. Epub 2019 Jan 31.

Department of Orthopaedics, Shaoxing People's Hospital, Zhejiang University School of Medicine, Shaoxing, China.

Bone fracture healing is a complex, dynamic process that involves various cell types, with osteoclasts and osteoblasts playing indispensable roles. In this study, we found that psoralen, the main active ingredient in Psoralea corylifolia L. fruit extract, enhanced bone fracture healing through activation of osteoclast and osteoblast activity via the ERK signaling pathway. Read More

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https://www.fasebj.org/doi/10.1096/fj.201801797R
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January 2019
8 Reads

Exercise training modifies gut microbiota with attenuated host responses to sepsis in wild-type mice.

FASEB J 2019 Jan 31:fj201802481R. Epub 2019 Jan 31.

College of Sport Science, Sungkyunkwan University, Suwon, South Korea.

This study investigated the effects of exercise preconditioning-induced modification in gut microbiota composition and host responses to cecal ligation and puncture (CLP)-induced sepsis. Four-week-old C57BL/6N male mice were randomly assigned to either CLP ( n = 30) or CLP-exercise (CLP+Exe; n = 30) groups. Prior to CLP-induced sepsis, the CLP+Exe mice were subjected to 8 wk of treadmill running. Read More

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January 2019
2 Reads