12,066 results match your criteria FASEB journal : official publication of the Federation of American Societies for Experimental Biology[Journal]


Narciclasine exerts anti-inflammatory actions by blocking leukocyte-endothelial cell interactions and down-regulation of the endothelial TNF receptor 1.

FASEB J 2019 Apr 24:fj201802440R. Epub 2019 Apr 24.

Institute of Pharmaceutical Biology, Faculty of Biochemistry, Chemistry and Pharmacy, Goethe University Frankfurt, Frankfurt, Germany.

The alkaloid narciclasine has been characterized extensively as an anticancer compound. Accumulating evidence suggests that narciclasine has anti-inflammatory potential; however, the underlying mechanism remains poorly understood. We hypothesized that narciclasine affects the activation of endothelial cells (ECs), a hallmark of inflammatory processes, which is a prerequisite for leukocyte-EC interaction. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201802440RDOI Listing

Wnt3a involved in the mechanical loading on improvement of bone remodeling and angiogenesis in a postmenopausal osteoporosis mouse model.

FASEB J 2019 Apr 24:fj201802711R. Epub 2019 Apr 24.

Department of Anatomy and Histology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China.

Osteoporosis is a major health problem, making bones fragile and susceptible to fracture. Previous works showed that mechanical loading stimulated bone formation and accelerated fracture healing. Focusing on the role of Wnt3a (wingless/integrated 3a), this study was aimed to assess effects of mechanical loading to the spine, using ovariectomized (OVX) mice as a model of osteoporosis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201802711RDOI Listing

The alliance between nerve fibers and stem cell populations in bone marrow: life partners in sickness and health.

FASEB J 2019 Apr 24:fj201900454R. Epub 2019 Apr 24.

Instituto de Investigação e Inovação em Saúde (i3S), Universidade do Porto, Porto, Portugal.

The bone marrow (BM) is the central hematopoietic organ in adult mammals, with great potential to be used as a tool to improve the efficacy of the body's response to a number of malignancies and stressful conditions. The nervous system emerges as a critical regulatory player of the BM both under homeostatic and pathologic settings, with essential roles in cellular anchorage and egress, stem cell differentiation, and endothelial cell permeability. This review collects the current knowledge on the interplay between the nervous system and the BM cell populations, with a focus on how the nervous system modulates hematopoietic stem and progenitor cell, mesenchymal stromal cell, and endothelial progenitor cell activity in BM. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201900454RDOI Listing

Correlative SICM-FCM reveals changes in morphology and kinetics of endocytic pits induced by disease-associated mutations in dynamin.

FASEB J 2019 Apr 24:fj201802635R. Epub 2019 Apr 24.

Division of Experimental Medicine, Department of Medicine, Imperial College London, London, United Kingdom.

Dynamin 2 (DNM2) is a GTP-binding protein that controls endocytic vesicle scission and defines a whole class of dynamin-dependent endocytosis, including clathrin-mediated endocytosis by caveoli. It has been suggested that mutations in the gene, associated with 3 inherited diseases, disrupt endocytosis. However, how exactly mutations affect the nanoscale morphology of endocytic machinery has never been studied. Read More

View Article

Download full-text PDF

Source
https://www.fasebj.org/doi/10.1096/fj.201802635R
Publisher Site
http://dx.doi.org/10.1096/fj.201802635RDOI Listing
April 2019
1 Read

Resolvin D2 elevates cAMP to increase intracellular [Ca] and stimulate secretion from conjunctival goblet cells.

FASEB J 2019 Apr 23:fj201802467R. Epub 2019 Apr 23.

Schepens Eye Research Institute, Massachusetts Eye and Ear-Harvard Medical School, Boston, Massachusetts, USA.

Under physiologic conditions, conjunctival goblet cells (CGCs) secrete mucins into the tear film to preserve ocular surface homeostasis. Specialized proresolving mediators (SPMs), like resolvins (Rvs), regulate secretion from CGCs and actively terminate inflammation. The purpose of this study was to determine if RvD2 stimulated mucin secretion and to investigate the cellular signaling components. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201802467RDOI Listing

The circadian clock gene is required to control the timing of retinal neurogenesis and lamination of Müller glia in the mouse retina.

FASEB J 2019 Apr 19:fj201801832RR. Epub 2019 Apr 19.

Department of Ophthalmic Research, Cole Eye Institute, Cleveland Clinic, Cleveland, Ohio, USA.

A single pool of multipotent retinal progenitor cells give rise to the diverse cell types within the mammalian retina. Such cellular diversity is due to precise control of various cellular processes like cell specification, proliferation, differentiation, and maturation. Circadian clock genes can control the expression of key regulators of cell cycle progression and therefore can synchronize the cell cycle state of a heterogeneous population of cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201801832RRDOI Listing
April 2019
1 Read

Myonectin deletion promotes adipose fat storage and reduces liver steatosis.

FASEB J 2019 Apr 19:fj201900520R. Epub 2019 Apr 19.

Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

We recently described myonectin (also known as erythroferrone) as a novel skeletal muscle-derived myokine with metabolic functions. Here, we use a genetic mouse model to determine myonectin's requirement for metabolic homeostasis. Female myonectin-deficient mice had larger gonadal fat pads and developed mild insulin resistance when fed a high-fat diet (HFD) and had reduced food intake during refeeding after an unfed period but were otherwise indistinguishable from wild-type littermates. Read More

View Article

Download full-text PDF

Source
https://www.fasebj.org/doi/10.1096/fj.201900520R
Publisher Site
http://dx.doi.org/10.1096/fj.201900520RDOI Listing
April 2019
1 Read

Mammary adipose stromal cells derived from obese women reduce sensitivity to the aromatase inhibitor anastrazole in an organotypic breast model.

FASEB J 2019 Apr 19:fj201802347RRR. Epub 2019 Apr 19.

Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Aromatase inhibitors are the preferred treatment for certain women with estrogen receptor (ER)-positive breast cancer, but evidence suggests that women with obesity experience aromatase inhibitor resistance at higher rates. To compare how stromal cells derived from women who are lean or obese influence response to the aromatase inhibitor (anastrazole), we incorporated patient-derived stroma in a previously characterized MCF7-derived duct model. Coculture with adipose stromal cells enabled the metabolism of testosterone (T) to E, which induced estrogen response element activity, epithelial proliferation, and hyperplasia in MCF7 cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201802347RRRDOI Listing

GdX/UBL4A-knockout mice resist collagen-induced arthritis by balancing the population of T1/T17 and regulatory T cells.

FASEB J 2019 Apr 19:fj201802217RR. Epub 2019 Apr 19.

State Key Laboratory of Membrane Biology, School of Medicine, Tsinghua University, Beijing, China.

Rheumatoid arthritis (RA) is an autoimmune disease associated with synovial hyperplasia and bone and cartilage destruction. T cells, notably T helper (T)-1 and T17 cells, play a critical role in the pathologic process of RA. However, it remains unclear how T1 and T17 cells are regulated during RA. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201802217RRDOI Listing

Deficiency of X-ray repair cross-complementing group 1 in primordial germ cells contributes to male infertility.

FASEB J 2019 Apr 18:fj201801962RR. Epub 2019 Apr 18.

State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing, China.

X-ray repair cross-complementing group 1 (), a key DNA repair gene, plays a vital role in maintaining genomic stability and is highly expressed in the early stages of spermatogenesis, but the exact functions remain elusive. Here we generated primordial germ cell-specific knockout (c) mice to elucidate the effects of on spermatogenesis. We demonstrated that deficiency results in infertility in male mice due to impaired spermatogenesis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201801962RRDOI Listing

MicroRNA-124 regulates the expression of p62/p38 and promotes autophagy in the inflammatory pathogenesis of Parkinson's disease.

FASEB J 2019 Apr 17:fj201900363R. Epub 2019 Apr 17.

Department of Neurosurgery, The First Affiliated Hospital of Nanchang University, Nanchang, China.

Parkinson's disease (PD) is a neurodegenerative disorder characterized by motor and nonmotor symptoms due to the selective loss of midbrain dopaminergic neurons. The evidence for a chronic inflammatory reaction mediated by microglial cells in the brain is particularly strong in PD. In our previous study, we have shown that brain-specific microRNA-124 (miR-124) is significantly down-regulated in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse model of PD and that it can also inhibit neuroinflammation during the development of PD. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201900363RDOI Listing

Human kallikrein overexpression alleviates cardiac aging by alternatively regulating macrophage polarization in aged rats.

FASEB J 2019 Apr 17:fj201802371RR. Epub 2019 Apr 17.

Department of Geriatric Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Cardiac aging is characterized by myocardial hypertrophy, fibrosis, and diastolic dysfunction. Human kallikrein (hKLK1) protects against fibrosis in various pathogenic states. However, the effects of hKLK1 overexpression on cardiac aging-related fibrosis and the underlying mechanisms remain unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201802371RRDOI Listing

Time-restricted release of multiple neurotrophic factors promotes axonal regeneration and functional recovery after peripheral nerve injury.

FASEB J 2019 Apr 17:fj201802065RR. Epub 2019 Apr 17.

Department of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.

Delivery of multiple neurotrophic factors (NTFs), especially with time-restricted release kinetics, holds great potential for nerve repair. In this study, we utilized the tetracycline-regulatable Tet-On 3G system to control the expression of c-Jun, which is a common regulator of multiple NTFs in Schwann cells (SCs). , Tet-On/c-Jun-modified SCs showed a tightly controllable secretion of multiple NTFs, including glial cell line-derived NTF, nerve growth factor, brain-derived NTF, and artemin, by the addition or removal of doxycycline (Dox). Read More

View Article

Download full-text PDF

Source
https://www.fasebj.org/doi/10.1096/fj.201802065RR
Publisher Site
http://dx.doi.org/10.1096/fj.201802065RRDOI Listing
April 2019
4 Reads

Deletion of maternal UHRF1 severely reduces mouse oocyte quality and causes developmental defects in preimplantation embryos.

FASEB J 2019 Apr 17:fj201801696RRRR. Epub 2019 Apr 17.

Department of Histology and Embryology, State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.

The ubiquitin-like, containing PHD and RING finger domains, 1 (UHRF1) protein recognizes DNA methylation and histone modification and plays a critical role in epigenetic regulation. Recently, UHRF1 was shown to have a role in DNA methylation in oocytes and early embryos. Here, we reveal that maternal UHRF1 determines the quality of mouse oocytes. Read More

View Article

Download full-text PDF

Source
https://www.fasebj.org/doi/10.1096/fj.201801696RRRR
Publisher Site
http://dx.doi.org/10.1096/fj.201801696RRRRDOI Listing
April 2019
2 Reads

Lgr5-overexpressing mesenchymal stem cells augment fracture healing through regulation of Wnt/ERK signaling pathways and mitochondrial dynamics.

FASEB J 2019 Apr 16:fj201900082RR. Epub 2019 Apr 16.

Department of Orthopaedics and Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong, China.

Fracture remains one of the most common traumatic conditions in orthopedic surgery. The use of mesenchymal stem cells (MSCs) to augment fracture repair is promising. Leucine-rich repeat-containing GPCR 5 (Lgr5), a transmembrane protein, has been identified as a novel adult stem cell marker in various organs and tissues. Read More

View Article

Download full-text PDF

Source
https://www.fasebj.org/doi/10.1096/fj.201900082RR
Publisher Site
http://dx.doi.org/10.1096/fj.201900082RRDOI Listing
April 2019
3 Reads

Undifferentiated spermatogonia regulate expression through NOTCH signaling and drive germ cell differentiation.

FASEB J 2019 Apr 16:fj201802361R. Epub 2019 Apr 16.

Department of Endocrine Neoplasia and Hormonal Disorders, University of Texas MD Anderson Cancer Center, Houston, Texas, USA.

Cytochrome P450 family 26 subfamily B member 1 (CYP26B1) regulates the concentration of all-trans retinoic acid (RA) and plays a key role in germ cell differentiation by controlling local distribution of RA. The mechanisms regulating expression in postnatal Sertoli cells, the main components of the stem cell niche, are so far unknown. During gonad development, expression of is maintained by Steroidogenic Factor 1 (SF-1) and Sex-Determining Region Y Box-9 (SOX9), which ensure that RA is degraded and germ cell differentiation is blocked. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201802361RDOI Listing
April 2019
1 Read

Losartan protects against myocardial ischemia and reperfusion injury vascular integrity preservation.

FASEB J 2019 Apr 16:fj201900060R. Epub 2019 Apr 16.

Key Laboratory of Molecular Biophysics of the Ministry of Education, Center for Human Genome Research, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.

Vascular hyperpermeability caused by distorted endothelial cell-cell junctions is associated with the no-reflow phenomenon after opening of the occluded vessel(s) in patients with coronary artery disease (CAD), the leading cause of death worldwide. Coronary no-reflow is observed in ∼30% of CAD patients after percutaneous coronary stenting and is associated with a worse prognosis at follow-up and a higher incidence of death. However, limited tools are available to control vascular hyperpermeability and no-reflow. Read More

View Article

Download full-text PDF

Source
https://www.fasebj.org/doi/10.1096/fj.201900060R
Publisher Site
http://dx.doi.org/10.1096/fj.201900060RDOI Listing
April 2019
3 Reads

Distinct expression pattern of periostin splice variants in chondrocytes and ligament progenitor cells.

FASEB J 2019 Apr 16:fj201802281R. Epub 2019 Apr 16.

Department of Orthopaedic Surgery, Musculoskeletal Research Center, Washington University School of Medicine, St. Louis, Missouri, USA.

Periostin (), a secretory matricellular matrix protein, plays a multitude of biologic functions. Various splice variants of have been described; however, their expression pattern and functional implications are not completely understood. This study was undertaken to decipher the differential expression pattern of and its splice variants in various tissues and cell types. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201802281RDOI Listing

NR2E3 is a key component in p53 activation by regulating a long noncoding RNA DINO in acute liver injuries.

FASEB J 2019 Apr 16:fj201801881RR. Epub 2019 Apr 16.

Department of Environmental Health, College of Medicine, University of Cincinnati, Cincinnati, Ohio, USA.

Damage-induced long noncoding RNA (DINO) is a long noncoding RNA that directly interacts with p53 and thereby enhances p53 stability and activity in response to various cellular stresses. Here, we demonstrate that nuclear receptor subfamily 2 group E member 3 (NR2E3) plays a crucial role in maintaining active DINO epigenetic status for its proper induction and subsequent p53 activation. In acetaminophen (APAP)- or carbon tetrachloride-induced acute liver injuries, NR2E3 knockout (KO) mice exhibited far more severe liver injuries due to impaired DINO induction and p53 activation. Read More

View Article

Download full-text PDF

Source
https://www.fasebj.org/doi/10.1096/fj.201801881RR
Publisher Site
http://dx.doi.org/10.1096/fj.201801881RRDOI Listing
April 2019
2 Reads

MAPK inhibition and growth hormone: a promising therapy in XLH.

FASEB J 2019 Apr 11:fj201802007R. Epub 2019 Apr 11.

Division of Pediatrics, Department of Medicine, Faculty of Medicine and Health Sciences, University of Oviedo, Oviedo, Spain.

X-linked hypophosphatemia (XLH) leads to growth retardation and bone deformities, which are not fully avoided by conventional treatment with phosphate and vitamin D analogs. Pediatric patients have been treated with growth hormone (GH), and recent findings suggest that blocking fibroblast growth factor 23 actions may be the most effective therapy, but its effects on growth are not known. We here report the effect of MAPK inhibition alone or associated with GH on growth and growth plate and bone structure of young Hyp (the XLH animal model) mice. Read More

View Article

Download full-text PDF

Source
https://www.fasebj.org/doi/10.1096/fj.201802007R
Publisher Site
http://dx.doi.org/10.1096/fj.201802007RDOI Listing
April 2019
2 Reads

Physiological levels of chromogranin A prevent doxorubicin-induced cardiotoxicity without impairing its anticancer activity.

FASEB J 2019 Apr 11:fj201802707R. Epub 2019 Apr 11.

Laboratory of Cellular and Molecular Cardiac Pathophysiology, Department of Biology, Ecology, and Earth Science, University of Calabria, Rende (Cosenza), Italy.

The clinical use of doxorubicin (Doxo), a widely used anticancer chemotherapeutic drug, is limited by dose-dependent cardiotoxicity. We have investigated whether chromogranin A (CgA), a cardioregulatory protein released in the blood by the neuroendocrine system and by the heart itself, may contribute to regulation of the cardiotoxic and antitumor activities of Doxo. The effects of a physiologic dose of full-length recombinant CgA on Doxo-induced cardiotoxicity and antitumor activity were investigated in rats using in vivo and ex vivo models and in murine models of melanoma, fibrosarcoma, lymphoma, and lung cancer, respectively. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201802707RDOI Listing
April 2019
1 Read

Dynamic changes in rumen fermentation and bacterial community following rumen fluid transplantation in a sheep model of rumen acidosis: implications for rumen health in ruminants.

FASEB J 2019 Apr 11:fj201802456R. Epub 2019 Apr 11.

Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.

Over the years, rumen fluid transplantation (RT) has been successfully applied to treat acute rumen acidosis in ruminants, but how it functions in the ruminal microbial homeostasis and host function remains largely unknown. Here, we investigated the dynamic changes of rumen fermentation and bacterial communities following RT and its beneficial effects on rumen epithelial morphology and function in a sheep model of rumen acidosis. The results showed that RT resulted in dynamic changes in rumen fermentation and increased the concentrations of total volatile fatty acid, acetate, propionate, and butyrate, but it decreased the levels of lactate and LPS in the rumen. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201802456RDOI Listing
April 2019
1 Read

Epigenetic-genetic chromatin footprinting identifies novel and subject-specific genes active in prefrontal cortex neurons.

FASEB J 2019 Apr 10:fj201802646R. Epub 2019 Apr 10.

Department of Psychiatry, University of Massachusetts Medical School, Worcester, Massachusetts, USA.

Human prefrontal cortex (PFC) is associated with broad individual variabilities in functions linked to personality, social behaviors, and cognitive functions. The phenotype variabilities associated with brain functions can be caused by genetic or epigenetic factors. The interactions between these factors in human subjects is, as of yet, poorly understood. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201802646RDOI Listing
April 2019
5 Reads

Apolipoprotein A-I improves pancreatic β-cell function independent of the ATP-binding cassette transporters ABCA1 and ABCG1.

FASEB J 2019 Apr 10:fj201802512RR. Epub 2019 Apr 10.

Lipid Research Group, School of Medical Sciences, Faculty of Medicine, University of New South Wales Sydney, Sydney, New South Wales, Australia.

Apolipoprotein A-I (apoA-I), the main protein constituent of HDLs, increases insulin synthesis and insulin secretion in pancreatic β cells. ApoA-I also accepts cholesterol that effluxes from cells expressing ATP-binding cassette transporter A1 (ABCA1) and ATP-binding cassette transporter G (ABCG1). Mice with conditional deletion of ABCA1 and ABCG1 in β cells [β-double knockout (DKO) mice] have increased islet cholesterol levels and reduced glucose-stimulated insulin secretion (GSIS). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201802512RRDOI Listing

Self-assembling peptide modified with QHREDGS as a novel delivery system for mesenchymal stem cell transplantation after myocardial infarction.

FASEB J 2019 Apr 10:fj201801768RR. Epub 2019 Apr 10.

Department of Anatomy, School of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

The lower cell survival and retention in the hostile microenvironment after transplantation has been implicated as a major bottleneck in the advancement of stem cell therapy for myocardial infarction (MI). In this study, we designed a novel self-assembling peptide (SAP) by attaching prosurvival peptide QHREDGS derived from angiopoeitin-1 to the known SAP, RADA16-I. The mesenchymal stem cells (MSCs) were harvested from male rats and cytoprotective effect of this designer SAP (DSAP) on cultured MSCs was detected by Hoechst 33342 staining after being exposed to oxygen and glucose deprivation (OGD). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201801768RRDOI Listing
April 2019
2 Reads

Supplementation with dietary ω-3 mitigates immobilization-induced reductions in skeletal muscle mitochondrial respiration in young women.

FASEB J 2019 Apr 10:fj201900095R. Epub 2019 Apr 10.

Department of Human Health and Nutritional Sciences, University of Guelph, Ontario, Canada.

Omega-3 (ω-3) supplementation attenuates immobilization-induced atrophy; however, the underlying mechanisms remain unclear. Since mitochondrial dysfunction and oxidative stress have been implicated in muscle atrophy, we examined whether ω-3 supplementation could mitigate disuse-mediated mitochondrial dysfunction. Healthy young women (age = 22 ± 3 yr) randomly received control ( n = 9) or ω-3 supplementation ( n = 11; 3 g eicosapentaenoic acid, 2 g docosahexaenoic acid) for 4 wk prior to and throughout 2 wk of single-limb immobilization. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201900095RDOI Listing

Exosomes from activated hepatic stellate cells contain GLUT1 and PKM2: a role for exosomes in metabolic switch of liver nonparenchymal cells.

FASEB J 2019 Apr 10:fj201802675R. Epub 2019 Apr 10.

Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

The mechanism of exosomes derived from activated hepatic stellate cells (HSCs) involved in liver fibrosis is poorly understood. We previously reported that hypoxia-inducible factor 1 (Hif-1) regulated HSC activation, and, therefore, we investigated in current work whether Hif-1 regulates exosome secretion and the metabolic switch of HSCs, thus affecting the metabolism of liver nonparenchymal cells. In this study, the characteristics of exosomes from HSCs were assessed via electron microscopy, Western blot analysis, and acetylcholinesterase activity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201802675RDOI Listing
April 2019
1 Read

Hypoxia-inducible factor 1α (HIF-1α) is a major determinant in the enhanced function of muscle-derived progenitors from MRL/MpJ mice.

FASEB J 2019 Apr 10:fj201801794R. Epub 2019 Apr 10.

Department of Orthopedic Surgery, McGovern Medical School, University of Texas Health Science Center-Houston, Houston, Texas, USA.

Although the mouse strain Murphy Roths Large (MRL/MpJ) possesses high regenerative potential, the mechanism of tissue regeneration, including skeletal muscle, in MRL/MpJ mice after injury is still unclear. Our previous studies have shown that muscle-derived stem/progenitor cell (MDSPC) function is significantly enhanced in MRL/MpJ mice when compared with MDSPCs isolated from age-matched wild-type (WT) mice. Using mass spectrometry-based proteomic analysis, we identified increased expression of hypoxia-inducible factor (HIF) 1α target genes (expression of glycolytic factors and antioxidants) in sera from MRL/MpJ mice compared with WT mice. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201801794RDOI Listing
April 2019
1 Read

The calmodulin-binding tetraleucine motif of KCNE4 is responsible for association with Kv1.3.

FASEB J 2019 Apr 10:fj201801164RR. Epub 2019 Apr 10.

Departament de Bioquímica i Biomedicina Molecular, Molecular Physiology Laboratory, Institut de Biomedicina (IBUB), Universitat de Barcelona, Barcelona, Spain.

The voltage-dependent potassium (Kv) channel Kv1.3 regulates leukocyte proliferation, activation, and apoptosis, and altered expression of this channel is linked to autoimmune diseases. Thus, the fine-tuning of Kv1. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201801164RRDOI Listing

MCPH1 is essential for cellular adaptation to the G-phase decatenation checkpoint.

FASEB J 2019 Apr 9:fj201802009RR. Epub 2019 Apr 9.

Departamento de Biología Experimental, Universidad de Jaén, Jaén, Spain.

Cellular checkpoints controlling entry into mitosis monitor the integrity of the DNA and delay mitosis onset until the alteration is fully repaired. However, this canonical response can weaken, leading to a spontaneous bypass of the checkpoint, a process referred to as checkpoint adaptation. Here, we have investigated the contribution of microcephalin 1 (MCPH1), mutated in primary microcephaly, to the decatenation checkpoint, a less-understood G pathway that delays entry into mitosis until chromosomes are properly disentangled. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201802009RRDOI Listing

4-Phenylbutyrate protects against atherosclerotic lesion growth by increasing the expression of HSP25 in macrophages and in the circulation of Apoe mice.

FASEB J 2019 Apr 9:fj201802293RR. Epub 2019 Apr 9.

Division of Nephrology, Department of Medicine, McMaster University-The Research Institute of St. Joe's Hamilton-Hamilton Centre for Kidney Research, Hamilton, Ontario, Canada; and.

Endoplasmic reticulum stress plays an important role in cardiovascular disease (CVD) and atherosclerosis. We aimed to assess the ability of 4-phenylbutyrate (4-PBA), a small chemical chaperone administered via drinking water, to reduce atherosclerotic lesion size in chow-fed apolipoprotein (Apo) e mice and to identify mechanisms that contribute to its antiatherogenic effect. Chow-fed 17-wk-old female Apoe mice treated with 4-PBA-supplemented drinking water for 5 wk exhibited smaller lesions as well as increased plasma levels of heat shock protein (HSP) 25, the mouse homolog of human HSP27, compared with controls. Read More

View Article

Download full-text PDF

Source
https://www.fasebj.org/doi/10.1096/fj.201802293RR
Publisher Site
http://dx.doi.org/10.1096/fj.201802293RRDOI Listing
April 2019
4 Reads
5.043 Impact Factor

Down-regulation of exosomal microRNA-224-3p derived from bone marrow-derived mesenchymal stem cells potentiates angiogenesis in traumatic osteonecrosis of the femoral head.

FASEB J 2019 Apr 9:fj201801618RRR. Epub 2019 Apr 9.

Department of Orthopaedics, Wuhan Third Hospital-Tongren Hospital of Wuhan University, Wuhan, China.

Traumatic osteonecrosis of the femoral head (ONFH) is a condition leading to the collapse of the femoral head, and the primary treatment is a total hip replacement, which has a poor prognosis. The current study was conducted with the aim of investigating the role of exosomes from bone marrow-derived mesenchymal stem cells (BM-MSCs) carrying microRNA-224-3p (miR-224-3p) in traumatic ONFH. Initially, a microarray analysis was performed to screen the differentially expressed genes and miRs associated with traumatic ONFH. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201801618RRRDOI Listing
April 2019
3 Reads

APOE genotype influences the gut microbiome structure and function in humans and mice: relevance for Alzheimer's disease pathophysiology.

FASEB J 2019 Apr 8:fj201900071R. Epub 2019 Apr 8.

Norwich Medical School, University of East Anglia, Norwich, United Kingdom; and.

Apolipoprotein E ( APOE) genotype is the strongest prevalent genetic risk factor for Alzheimer's disease (AD). Numerous studies have provided insights into the pathologic mechanisms. However, a comprehensive understanding of the impact of APOE genotype on microflora speciation and metabolism is completely lacking. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201900071RDOI Listing
April 2019
2 Reads

Scleraxis lineage cells contribute to organized bridging tissue during tendon healing and identify a subpopulation of resident tendon cells.

FASEB J 2019 Apr 5:fj201900130RR. Epub 2019 Apr 5.

Center for Musculoskeletal Research, Department of Orthopaedics and Rehabilitation, University of Rochester Medical Center, Rochester, New York, USA.

During tendon healing, it is postulated that tendon cells drive tissue regeneration, whereas extrinsic cells drive pathologic scar formation. Tendon cells are frequently described as a homogenous, fibroblast population that is positive for the marker Scleraxis (Scx). It is controversial whether tendon cells localize within the forming scar tissue during adult tendon healing. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201900130RRDOI Listing

Selective inhibition of class IIa histone deacetylases alleviates renal fibrosis.

FASEB J 2019 Apr 5:fj201801067RR. Epub 2019 Apr 5.

Department of Medicine, Rhode Island Hospital-The Warren Alpert Medical School, Brown University, Providence, Rhode Island, USA.

In this study, we examined the effect of MC1568, a selective class IIa histone deacetylase (HDAC) inhibitor, on the development and progression of renal fibrosis in a murine model of renal fibrosis induced by unilateral ureteral obstruction (UUO). All 4 class IIa HDAC isoforms, in particular HDAC4, were up-regulated in renal epithelial cells of the injured kidney. Administration of MC1568 immediately after UUO injury reduced expression of α-smooth muscle actin (α-SMA), fibronectin, and collagen 1. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201801067RRDOI Listing
April 2019
1 Read
5.043 Impact Factor

Integrative analysis of the forkhead box A2 (FOXA2) cistrome for the human endometrium.

FASEB J 2019 Apr 5:fj201900013R. Epub 2019 Apr 5.

Division of Animal Sciences, University of Missouri, Columbia, Missouri, USA.

The pioneer forkhead box (FOX)A2 transcription factor is specifically expressed in the glands of the uterus, which are central to endometrial function and fertility. In mice, FOXA2 is a critical regulator of uterine gland development in the neonate and gland function in the adult. An integrative approach was used here to define the FOXA2 cistrome in the human endometrium. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201900013RDOI Listing
April 2019
5.043 Impact Factor

The immunoregulatory effects of CD8 T-cell-derived perforin on diet-induced nonalcoholic steatohepatitis.

FASEB J 2019 Apr 5:fj201802534RR. Epub 2019 Apr 5.

Experimental and Translational Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

The liver is a central immunologic organ with a high density of myeloid and lymphoid immune cells that play important roles in the development and progression of nonalcoholic steatohepatitis (NASH). However, the immune-cell-mediated regulation of NASH and its underlying mechanisms remain obscure. In this study, Prf1 mice showed significantly higher plasma alanine transaminase levels, with increased liver fat accumulation, lobular inflammation, and focal necrosis compared with wild-type (WT) mice after 4 wk of feeding on a methionine- and choline-deficient diet (MCD) or 16 wk of feeding on a high-fat diet. Read More

View Article

Download full-text PDF

Source
https://www.fasebj.org/doi/10.1096/fj.201802534RR
Publisher Site
http://dx.doi.org/10.1096/fj.201802534RRDOI Listing
April 2019
3 Reads

Targeted gene insertion into Z chromosome of chicken primordial germ cells for avian sexing model development.

FASEB J 2019 Apr 5:fj201802671R. Epub 2019 Apr 5.

Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, South Korea.

Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) have facilitated the production of genome-edited animals for use as models. Because of their unique developmental system, avian species offer many advantages as model vertebrates. Here, we report the development of novel chicken models using the CRISPR/Cas9-mediated nonhomologous end joining repair pathway in chicken primordial germ cells (PGCs). Read More

View Article

Download full-text PDF

Source
https://www.fasebj.org/doi/10.1096/fj.201802671R
Publisher Site
http://dx.doi.org/10.1096/fj.201802671RDOI Listing
April 2019
18 Reads
5.043 Impact Factor

Lactate-induced activation of HCA2 improves survival in mice with sepsis.

FASEB J 2019 Apr 5:fj201801982R. Epub 2019 Apr 5.

Division of Nephrology, Beth Israel Deaconess Medical Center-Harvard Medical School, Boston, Massachusetts, USA.

Sepsis is characterized by systemic inflammation that is caused by infection and by activation of proinflammatory pathways, resulting in mitochondrial and cellular dysfunction leading to multiorgan failure. Here, we show the following: 1) in peritoneal immune cells, particularly macrophages, from mice that have undergone cecal ligation and puncture (CLP), hydroxycarboxylic acid receptor 2 (HCA2) expression increased in parallel with proinflammatory cytokines; 2) post-CLP survival rates of Hca2 knockout mice ( n = 22) were lower than those of wild-type (WT) mice ( n = 15) ( P < 0.011), which is suggestive of a protective role for HCA2 in sepsis; 3) WT mice subjected to CLP-induced sepsis and treated with lactated Ringer's solution (LR, n = 13) survived longer than those treated with normal saline ( n = 14; P < 0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201801982RDOI Listing
April 2019
2 Reads

Statin-dependent modulation of mitochondrial metabolism in cancer cells is independent of cholesterol content.

FASEB J 2019 Apr 5:fj201802723R. Epub 2019 Apr 5.

Department of Drug Discovery and Biomedical Sciences, Medical University of South Carolina, Charleston, South Carolina, USA.

Statins, widely used to treat hypercholesterolemia, inhibit the 3-hydroxy-3-methylglutaryl-coenzyme A reductase, the rate-limiting enzyme of de novo cholesterol (Chol) synthesis. Statins have been also reported to slow tumor progression. In cancer cells, ATP is generated both by glycolysis and oxidative phosphorylation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201802723RDOI Listing
April 2019
1 Read

The role of FHL2 in wound healing and inflammation.

Authors:
Viktor Wixler

FASEB J 2019 Apr 2:fj201802765RR. Epub 2019 Apr 2.

Centre for Molecular Biology of Inflammation, Institute of Molecular Virology, Westfaelische Wilhelms University Muenster, Muenster, Germany.

The 4-and-a-half LIM domain protein 2 (FHL2) is a multifunctional adaptor protein that can interact with cell surface receptors, cytosolic adaptor and structural proteins, kinases, and nuclear transcription factors. It is involved in numerous functional activities, including the epithelial-mesenchymal transition, cell proliferation, apoptosis, adhesion, migration, structural stability, and gene expression. Despite this, FHL2-knockout (KO) mice are viable and fertile with no obvious abnormalities, rather suggesting a high capacity for fine-tuning adjustment and functional redundancy of FHL2. Read More

View Article

Download full-text PDF

Source
https://www.fasebj.org/doi/10.1096/fj.201802765RR
Publisher Site
http://dx.doi.org/10.1096/fj.201802765RRDOI Listing
April 2019
8 Reads

Reduced mitochondrial lipid oxidation leads to fat accumulation in myosteatosis.

FASEB J 2019 Apr 2:fj201802457RR. Epub 2019 Apr 2.

Department of Orthopedic Surgery, University of Michigan, Ann Arbor, Michigan, USA.

Myosteatosis is the pathologic accumulation of lipid that can occur in conjunction with atrophy and fibrosis following skeletal muscle injury. Little is known about the mechanisms by which lipid accumulates in myosteatosis, but many clinical studies have demonstrated that the degree of lipid infiltration negatively correlates with muscle function and regeneration. Our objective was to determine the pathologic changes that result in lipid accumulation in injured muscle fibers. Read More

View Article

Download full-text PDF

Source
https://www.fasebj.org/doi/10.1096/fj.201802457RR
Publisher Site
http://dx.doi.org/10.1096/fj.201802457RRDOI Listing
April 2019
7 Reads

PDH-mediated metabolic flow is critical for skeletal muscle stem cell differentiation and myotube formation during regeneration in mice.

FASEB J 2019 Apr 2:fj201802479R. Epub 2019 Apr 2.

Department of Growth Regulation, Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, Japan.

Skeletal muscle satellite cells (SMSCs), the major stem cells responsible for the regeneration of skeletal muscle, are normally cell cycle arrested but differentiate to generate myocytes upon muscle damage, forming new myofibers along with self-renewing stem cells in preparation for subsequent injury. In this study, we investigated which factors stimulate the proliferation and differentiation of SMSCs and found that pyruvate, the end product of glycolysis, stimulates their differentiation. Pyruvate antagonizes the effects of hypoxia on preferential self-renewal of SMSCs through dephosphorylation or activation of pyruvate dehydrogenase (PDH), which mediates opening of the gateway from glycolysis to the tricarboxylic acid (TCA) cycle by producing acetyl coenzyme A from pyruvate. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201802479RDOI Listing

Resveratrol targets TyrRS acetylation to protect against radiation-induced damage.

FASEB J 2019 Apr 2:fj201802474RR. Epub 2019 Apr 2.

Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences-Peking Union Medical College, Tianjin, China.

Resveratrol (RSV) has broad prospective applications as a radiation protection drug, but its mechanism of action is not yet clear. Here, we found that 5 μM RSV can effectively reduce the cell death caused by irradiation. Irradiation leads to G/M phase arrest in the cell cycle, whereas RSV treatment increases S-phase cell cycle arrest, which is associated with sirtuin 1 (SIRT1) regulation. Read More

View Article

Download full-text PDF

Source
https://www.fasebj.org/doi/10.1096/fj.201802474RR
Publisher Site
http://dx.doi.org/10.1096/fj.201802474RRDOI Listing
April 2019
3 Reads

Fructose malabsorption induces cholecystokinin expression in the ileum and cecum by changing microbiota composition and metabolism.

FASEB J 2019 Apr 2:fj201801526RR. Epub 2019 Apr 2.

Micalis Institute, Institut National de la Recherche Agronomique (INRA), AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.

Current fructose consumption levels often overwhelm the intestinal capacity to absorb fructose. We investigated the impact of fructose malabsorption on intestinal endocrine function and addressed the role of the microbiota in this process. To answer this question, a mouse model of moderate fructose malabsorption [ketohexokinase mutant (KHK)] and wild-type (WT) littermate mice were used and received a 20%-fructose (KHK-F and WT-F) or 20%-glucose diet. Read More

View Article

Download full-text PDF

Source
https://www.fasebj.org/doi/10.1096/fj.201801526RR
Publisher Site
http://dx.doi.org/10.1096/fj.201801526RRDOI Listing
April 2019
5 Reads

Natural disease history of the D2 -mdx mouse model for Duchenne muscular dystrophy.

FASEB J 2019 Apr 1:fj201802488R. Epub 2019 Apr 1.

Department of Human Genetics, Leiden University Medical Centre, Leiden, The Netherlands.

The C57BL/10ScSn- Dmd/J (BL10- mdx) mouse has been the most commonly used model for Duchenne muscular dystrophy (DMD) for decades. Their muscle dysfunction and pathology is, however, less severe than in patients with DMD, which complicates preclinical studies. Recent discoveries indicate that disease severity is exacerbated when muscular dystrophy mouse models are generated on a DBA2/J genetic background. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201802488RDOI Listing
April 2019
2 Reads

Biophysical Mechanism of Protein Export by Bacterial Type III Secretion System.

Chem Pharm Bull (Tokyo) 2019 ;67(4):341-344

Department of Biophysical Chemistry, Kyoto Pharmaceutical University.

Type III secretion system (T3SS) is a protein injection nano-machine consisting of syringe and needle-like structure spanning bacterial inner and outer membranes. Bacteria insert the tip of T3SS needle to host cell membranes, and deliver effector proteins directly into host cells via T3SS to prime the host cell environment for infection. Thus inhibition of T3SS would be a potent strategy for suppressing bacterial infection. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1248/cpb.c18-00947DOI Listing
January 2019

Ingestion of resistant starch by mice markedly increases microbiome-derived metabolites.

FASEB J 2019 Mar 29:fj201900177R. Epub 2019 Mar 29.

Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia.

Recent research has shown significant health benefits deriving from high-dietary fiber or microbiome-accessible carbohydrate consumption. Compared with native starch (NS), dietary resistant starch (RS) is a high microbiome-accessible carbohydrate that significantly alters the gut microbiome. The aim of this study was to determine the systemic metabolic effects of high microbiome-accessible carbohydrate. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201900177RDOI Listing
March 2019
5.043 Impact Factor

Ursodeoxycholic acid inhibits uptake and vasoconstrictor effects of taurocholate in human placenta.

FASEB J 2019 Mar 28:fj201900015RR. Epub 2019 Mar 28.

Faculty of Medicine, University of Southampton, Southampton, United Kingdom.

Intrahepatic cholestasis of pregnancy (ICP) causes increased transfer of maternal bile acids to the fetus and an increased incidence of sudden fetal death. Treatment includes ursodeoxycholic acid (UDCA), but it is not clear if UDCA protects the fetus. This study explores the placental transport of the bile acid taurocholate (TC) by the organic anion-transporting polypeptide, (OATP)4A1, its effects on the placental proteome and vascular function, and how these are modified by UDCA. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201900015RRDOI Listing
March 2019
1 Read

Adipose glucocorticoid action influences whole-body metabolism via modulation of hepatic insulin action.

FASEB J 2019 Mar 28:fj201802706R. Epub 2019 Mar 28.

Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut, USA.

The connection between adipose glucocorticoid action and whole-body metabolism is incompletely understood. Thus, we generated adipose tissue-specific glucocorticoid receptor-knockout (Ad-GcR) mice to explore potential mechanisms. Ad-GcR mice had a lower concentration of fasting plasma nonesterified fatty acids and less hepatic steatosis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201802706RDOI Listing