1,711 results match your criteria Expert Review of Molecular Diagnostics [Journal]


Metabolomic insights into pathophysiological mechanisms and biomarker discovery in clear cell - renal cell carcinoma.

Expert Rev Mol Diagn 2019 Apr 13. Epub 2019 Apr 13.

a Department of Emergency and Organ Transplantation - Urology, Andrology and Kidney Transplantation Unit , University of Bari , Bari , Italy.

Introduction: Clear cell renal cell carcinoma (ccRCC) is a metabolic disease, of which the incidence rate is increasing worldwide. Renal carcinoma is characterized by mutations in target genes involved in metabolic pathways. Metabolic reprogramming covers different processes such as aerobic glycolysis, fatty acid metabolism, and the utilization of tryptophan, glutamine, and arginine. Read More

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http://dx.doi.org/10.1080/14737159.2019.1607729DOI Listing
April 2019
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Clinical implementation of molecular methods in detection of microorganisms from blood with a special focus on PCR electrospray ionization mass spectrometry.

Expert Rev Mol Diagn 2019 Apr 12. Epub 2019 Apr 12.

c Department of Infectious Diseases , Karolinska University Hospital , Stockholm , Sweden.

Introduction: The ongoing improvement and development of state-of-the-art diagnostic methods indicate that we are in an era of revolution in clinical microbiological diagnosis of infectious diseases. Non-culture-based methods have the possibility to play a central role in delivering personalized microbiological diagnoses of severe infections. The PCR electrospray ionization mass spectrometry (PCR/ESI-MS) system is built on the principle of universal detection and specific identification. Read More

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http://dx.doi.org/10.1080/14737159.2019.1607728DOI Listing
April 2019
1 Read

Advanced multi-modal diagnostic approaches for detection of lung cancer.

Expert Rev Mol Diagn 2019 Apr 12. Epub 2019 Apr 12.

a Shobhaben Pratapbhai Patel School of Pharmacy and Technology Management , Shri Vile Parle Kelavani Mandal'S Narsee Monjee Institute of Management Studies University , V.L. Mehta Road, Vile Parle (W), Mumbai , Maharashtra 400056 , India .

Introduction: Lung cancer (LC) emerges as a principle cause of death among smokers and is also one of the most lethal forms of cancer in non-smokers. LC is mainly classified as non-small cell lung cancer (NSCLC), small cell lung cancer and lung carcinoid tumor. NSCLC is the most prevalent form of LC and its early stage diagnosis is essential to reduce mortality rate of patients and provide specific therapy. Read More

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http://dx.doi.org/10.1080/14737159.2019.1607299DOI Listing
April 2019
2 Reads

Known epigenetic biomarkers for prostate cancer detection and management: exploring the potential of blood-based liquid biopsies.

Expert Rev Mol Diagn 2019 Apr 8:1-9. Epub 2019 Apr 8.

a Cancer Biology and Epigenetics Group, IPO Porto Research Center (CI-IPOP) , Portuguese Oncology Institute of Porto (IPO Porto) , Porto , Portugal.

Introduction: Although prostate cancer (PCa) stands as an important cause of cancer-related deaths, a sizeable proportion of diagnosed cases are clinically insignificant. Hence, novel and more specific biomarkers to identify clinically significant PCa are needed. Liquid biopsies offer the potential to accurately identify cancer markers, including PCa. Read More

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http://dx.doi.org/10.1080/14737159.2019.1604224DOI Listing

Metabolomic profiling for the identification of novel diagnostic markers in prostate cancer: an update.

Expert Rev Mol Diagn 2019 Apr 8. Epub 2019 Apr 8.

a Department of Emergency and Organ Transplantation - Urology, Andrology and Kidney Transplantation Unit , University of Bari , Bari , Italy.

Introduction: An altered metabolic regulation is involved in the development and progression of different cancer types. As well as this, many genes associated with tumors are shown to have an important role in control of the metabolism. The incidence of prostate cancer (PCa) is increased in men with metabolic disorders. Read More

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http://dx.doi.org/10.1080/14737159.2019.1604223DOI Listing
April 2019
3 Reads

MicroRNAs in rheumatoid arthritis: what is the latest with regards to diagnostics?

Expert Rev Mol Diagn 2019 Apr 8:1-4. Epub 2019 Apr 8.

c Section Rheumatology Research , BGI Genomics, BGI-Shenzhen , Shenzhen , China.

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http://dx.doi.org/10.1080/14737159.2019.1599716DOI Listing
April 2019
1 Read

Liquid biopsy challenge and hope in colorectal cancer.

Expert Rev Mol Diagn 2019 Apr 4:1-8. Epub 2019 Apr 4.

h Pharmaceutical Chemistry Department, Faculty of Pharmacy , "Iuliu Haţieganu" University of Medicine and Pharmacy , Cluj-Napoca , Romania.

Introduction: Colorectal cancer is an important global health burden marked by a high mortality rate. Medical attention is drawn more often by the new targeted therapies, but also by the concept of liquid biopsy. Tumor's genetic profile is the major indicator of the response to targeted therapies and the risk for metastatic relapse. Read More

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http://dx.doi.org/10.1080/14737159.2019.1597708DOI Listing
April 2019
1 Read
3.516 Impact Factor

Recent advances in the development and evaluation of molecular diagnostics for Ebola virus disease.

Expert Rev Mol Diagn 2019 Mar 27:1-16. Epub 2019 Mar 27.

a HerpeZ , University Teaching hospital , Lusaka , Zambia.

Introduction: The 2014-16 outbreak of ebola virus disease (EVD) in West Africa resulted in 11,308 deaths. During the outbreak only 60% of patients were laboratory confirmed and global health authorities have identified the need for accurate and readily deployable molecular diagnostics as an important component of the ideal response to future outbreaks, to quickly identify and isolate patients. Areas covered: Currently PCR-based techniques and rapid diagnostic tests (RDTs) that detect antigens specific to EVD infections dominate the diagnostic landscape, but recent advances in biosensor technologies have led to novel approaches for the development of EVD diagnostics. Read More

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http://dx.doi.org/10.1080/14737159.2019.1595592DOI Listing
March 2019
3 Reads
3.516 Impact Factor

Advances in prognostic markers for colorectal cancer.

Expert Rev Mol Diagn 2019 Mar 25:1-12. Epub 2019 Mar 25.

a State Key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases, and Xijing Hospital of Digestive Diseases , Air Force Military Medical University , Xi'an , China.

Introduction: Colorectal cancer (CRC) is one of the most common malignant tumors worldwide and contributes significantly to cancer-related deaths. Since CRC is largely asymptomatic until alarm features develop to advanced stages, the incidence and mortality rate of CRC is relatively high and increases year by year. Areas covered: This review mainly summarizes the recent advances of genetic, epigenetic, and proteomic molecular markers that may be used to predict the prognosis of CRC. Read More

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http://dx.doi.org/10.1080/14737159.2019.1592679DOI Listing

Liquid biopsy in newly diagnosed patients with locoregional (I-IIIA) non-small cell lung cancer.

Expert Rev Mol Diagn 2019 Mar 24:1-9. Epub 2019 Mar 24.

a Department of Thoracic Surgery , Peking University People's Hospital , Beijing , P.R. China.

Introduction: Liquid biopsy is a promising method for the management of lung cancer, but previous studies focused mainly on patients with advanced-stage disease. As the methodology has progressed for the detection of circulating tumor DNA (ctDNA) and its aberrant methylation, researchers are gradually investigating the utility of liquid biopsy in early-stage patients. As a result, liquid biopsy has shown its potential for the application in patients with early- and locally advanced-stage non-small cell lung cancer (NSCLC). Read More

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http://dx.doi.org/10.1080/14737159.2019.1599717DOI Listing
March 2019
1 Read
3.516 Impact Factor

Recent advances in the molecular landscape of lung neuroendocrine tumors.

Expert Rev Mol Diagn 2019 Mar 22:1-17. Epub 2019 Mar 22.

p Christian CRUK and NIHR Southamtpon Experimental Cancer Medicine Centre, Faculty of Medicine , University of Southampton , Southampton , UK.

Introduction: Neuroendocrine tumors of the lung (Lung-NETs) make up a heterogenous family of neoplasms showing neuroendocrine differentiation and encompass carcinoids and neuroendocrine carcinomas. On molecular grounds, they considered two completely distinct and separate tumor groups with no overlap of molecular alterations nor common developmental mechanisms. Areas covered: Two perspectives were evaluated based on an extensive review and rethinking of literature: (1) the current classification as an instrument to obtaining clinical and molecular insights into the context of Lung-NETs; and (2) an alternative and innovative interpretation of these tumors, proposing a tripartite separation into early aggressive primary high-grade neuroendocrine tumors (HGNET), differentiating or secondary HGNET, and indolent NET. Read More

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http://dx.doi.org/10.1080/14737159.2019.1595593DOI Listing
March 2019
5 Reads

How to discover new antibiotic resistance genes?

Expert Rev Mol Diagn 2019 Mar 21:1-14. Epub 2019 Mar 21.

a Microbes Evolution Phylogeny and Infections (MEPHI), IRD, APHM, IHU Méditerranée Infection, Faculté de Médecine et de Pharmacie , Aix-Marseille-Univ , Marseille , France.

Introduction: Antibiotic resistance (AR) is a worldwide concern and the description of AR have been discovered mainly because of their implications in human medicine. Since the recent burden of whole-genome sequencing of microorganisms, the number of new AR genes (ARGs) have dramatically increased over the last decade. Areas covered: In this review, we will describe the different methods that could be used to characterize new ARGs using classic or innovative methods. Read More

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http://dx.doi.org/10.1080/14737159.2019.1592678DOI Listing
March 2019
1 Read

Diagnostic utility of kappa free light chains in multiple sclerosis.

Expert Rev Mol Diagn 2019 Feb 22:1-3. Epub 2019 Feb 22.

a Heller Institute of Medical Research, Sheba Medical Center , Tel-Hashomer , Ramat Gan , Israel.

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http://dx.doi.org/10.1080/14737159.2019.1586535DOI Listing
February 2019

An update on extracellular vesicles in multiple myeloma: a focus on their role in cell-to-cell cross-talk and as potential liquid biopsy biomarkers.

Expert Rev Mol Diagn 2019 Mar 26;19(3):249-258. Epub 2019 Feb 26.

b Hematology and Stem Cell Transplantation Unit , IRCCS-Referral Cancer Center of Basilic`ata (CROB) , Rionero in Vulture (PZ) , Italy.

Introduction: Multiple myeloma (MM) is characterized by a clonal proliferation of neoplastic plasma cells (PCs) in bone marrow (BM) and the interplay between MM PCs and the BM microenvironment, which plays a relevant role in its pathogenesis. In this important cross-talk, extracellular vesicles (EVs) are active. EVs, including small and medium/large EVs, are lipid bi-layer particles released in circulation by normal and neoplastic cells. Read More

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http://dx.doi.org/10.1080/14737159.2019.1583103DOI Listing
March 2019
3 Reads

Quantitative real-time PCR for differential diagnostics of parvovirus B19 infection in acute liver failure patients.

Expert Rev Mol Diagn 2019 Mar 26;19(3):259-266. Epub 2019 Feb 26.

a Laboratório de Desenvolvimento Tecnológico em Virologia , Instituto Oswaldo cruz, Fundação Oswaldo Cruz , Rio de Janeiro , Brasil.

Background: Human Parvovirus B19 (B19V) is a common pathogen worldwide. After primary infection, B19V-DNA may permanently persist in non-erythroid tissues, including the liver of patients with acute liver failure (ALF).

Objective: To validate a real-time PCR (qPCR) for the quantification of B19V-DNA, in order to establish a differential diagnosis for B19V infection in ALF patients. Read More

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http://dx.doi.org/10.1080/14737159.2019.1582333DOI Listing
March 2019
1 Read

Early detection and monitoring of cancer in liquid biopsy: advances and challenges.

Expert Rev Mol Diagn 2019 Feb 13:1-4. Epub 2019 Feb 13.

b Department of Clinical Oncology , Queen Elizabeth Hospital , Kowloon , Hong Kong.

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http://dx.doi.org/10.1080/14737159.2019.1583104DOI Listing
February 2019
3.516 Impact Factor

Prognostic significance of gamma-glutamyl transferase in patients with metastatic non-small cell lung cancer.

Expert Rev Mol Diagn 2019 Mar 15;19(3):267-272. Epub 2019 Feb 15.

b Department of Medical Oncology , Gazi University Faculty of Medicine , Ankara , Turkey.

Background: The prognostic significance of serum gamma-glutamyl transferase (GGT) level at diagnosis in patients with metastatic non-small lung cancer (NSCLC) is not clear. We aimed to assess the relationship between serum GGT level and overall survival (OS) and progression-free survival (PFS) in this patient population.

Methods: Data of patients with metastatic NSCLC who were admitted to the medical oncology clinic of our hospital during April 2013-December 2017 were retrospectively analyzed. Read More

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http://dx.doi.org/10.1080/14737159.2019.1579644DOI Listing
March 2019
2 Reads

The expanding use of matrix-assisted laser desorption/ionization-time of flight mass spectroscopy in the diagnosis of patients with mycotic diseases.

Expert Rev Mol Diagn 2019 Mar 5;19(3):241-248. Epub 2019 Feb 5.

b Division of General Internal Medicine , Weill Cornell Medicine of Cornell University , New York , NY , USA.

Introduction: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a powerful new tool to identify human fungal pathogens and has radically altered the diagnostic mycology workflow at many medical centers around the world. Areas covered: While most experience is with the identification of yeasts, including species of Candida and Cryptococcus, there is ongoing work investigating the role of MALDI-TOF MS to detect molds, including species of Aspergillus, Fusarium, Scedosporium, and Mucormyctes as well as thermally dimorphic fungi. Expert commentary: In this paper, we review the current knowledge about this important new platform and examine how its expanding use may impact molecular diagnostics and patient care in the years ahead. Read More

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http://dx.doi.org/10.1080/14737159.2019.1574572DOI Listing
March 2019
1 Read

From next-generation sequencing to targeted treatment of non-acquired epilepsies.

Expert Rev Mol Diagn 2019 Mar 4;19(3):217-228. Epub 2019 Feb 4.

a Department of Epilepsy Genetics and Precision Medicine , The Danish Epilepsy Centre , Dianalund , Denmark.

Introduction: Within the last decade, next-generation sequencing (NGS) has resulted in remarkable advances in the field of epilepsy genetics. NGS has become a routine part of the diagnostic workup in many countries. A workup that has led to higher diagnostic yields and insights into the underlying disease mechanisms. Read More

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http://dx.doi.org/10.1080/14737159.2019.1573144DOI Listing
March 2019
1 Read

The importance of pre- and post-test counseling for prenatal cell-free DNA screening for common fetal aneuploidies.

Expert Rev Mol Diagn 2019 Mar 7;19(3):201-215. Epub 2019 Feb 7.

a Department of Obstetrics and Gynecology , Fetomaternal Medical Center, Helsinki University and Helsinki University Hospital , Helsinki , Finland.

Introduction: Prenatal cell-free DNA screening for common fetal aneuploidies has rapidly changed the paradigm of prenatal care. Despite its advantages compared to conventional screening methods, its unexpectedly rapid implementation in clinical practice has generated several ethical and medical issues and misconceptions. Aggressive commercial marketing of cell-free DNA screening and media dissemination of misleading information have added confusion. Read More

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http://dx.doi.org/10.1080/14737159.2019.1571912DOI Listing
March 2019
6 Reads

Soluble CD163 is a potential biomarker in systemic sclerosis.

Expert Rev Mol Diagn 2019 Mar 22;19(3):197-199. Epub 2019 Jan 22.

a Department of Dermatology, Faculty of Medicine, Institute of Medical, Pharmaceutical and Health Sciences , Kanazawa University , Kanazawa , Japan.

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http://dx.doi.org/10.1080/14737159.2019.1571911DOI Listing
March 2019
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Loop-mediated isothermal amplification assay as a point-of-care diagnostic tool for Vibrio parahaemolyticus: recent developments and improvements.

Expert Rev Mol Diagn 2019 Mar 28;19(3):229-239. Epub 2019 Jan 28.

a Division of Infectious Diseases , Nitte University Centre for Science Education and Research , Mangaluru , India.

Introduction: A number of DNA-based diagnostic tools have been developed for the detection of Vibrio parahaemolyticus in seafood. However, the loop-mediated isothermal amplification (LAMP) has distinct advantages with regards to its simplicity, speed and the ease of performing without any need for sophisticated equipment. Over the last decade, LAMP has emerged as a potential tool for the detection of V. Read More

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https://www.tandfonline.com/doi/full/10.1080/14737159.2019.1
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http://dx.doi.org/10.1080/14737159.2019.1571913DOI Listing
March 2019
2 Reads

Technical considerations for circulating tumor DNA detection in oncology.

Expert Rev Mol Diagn 2019 02 23;19(2):121-135. Epub 2019 Jan 23.

b Service de Biopathologie , Institut de Cancérologie de Lorraine, Université de Lorraine, CNRS UMR 7039 CRAN , Nancy , France.

Introduction: Blood draw or collection of other body fluids, known as 'liquid biopsies' are generally less invasive procedures than tumor biopsies. Cell-free tumor DNA (ctDNA) is widely evaluated in cancer for early detection, diagnosis, prognosis, therapy monitoring or determination of minimal residual disease. In body fluid samples, ctDNA can represent a small fraction of total cell-free DNA (cfDNA), requiring highly sensitive assays. Read More

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http://dx.doi.org/10.1080/14737159.2019.1568873DOI Listing
February 2019
10 Reads

Promoter methylation of galanin receptors as epigenetic biomarkers for head and neck squamous cell carcinomas.

Expert Rev Mol Diagn 2019 02 21;19(2):137-148. Epub 2019 Jan 21.

f Laboratory of Head and Neck Center Biology, Department of Otolaryngology, Head and Neck Surgery , The University of Michigan , Ann Arbor , MI , USA.

Introduction: While remarkable progress has been made in standard treatments for head and neck squamous cell carcinomas (HNSCCs), the long-term survival remains at an unsatisfactory 40-50%. To improve the survival rate, biomarkers for optimal treatment selection and prognostic prediction, as well as novel, low-toxicity treatment strategies, are required. Galanin receptor (GALR) 1 and GALR2 are well-studied tumor suppressors in HNSCCs. Read More

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http://dx.doi.org/10.1080/14737159.2019.1567334DOI Listing
February 2019
2 Reads

Emerging methodologies for pathogen identification in bloodstream infections: an update.

Expert Rev Mol Diagn 2019 02 21;19(2):161-173. Epub 2019 Jan 21.

b Aix Marseille Univ, IRD, AP-HM, SSA, VITROME , Marseille , France.

Introduction: Bloodstream infections (BSIs) remain a major public health burden worldwide, particularly in high-income countries as they are associated with a significant mortality rate. As early administration of appropriate antimicrobial therapy is a major prognostic factor, there remain unmet needs for shortening BSI diagnosis. Current blood cultures (BC) processing to identify pathogens involved in BSI is not compatible with such delays, although it remains the gold standard. Read More

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http://dx.doi.org/10.1080/14737159.2019.1568241DOI Listing
February 2019
10 Reads

Practical applications of DNA genotyping in diagnostic pathology.

Authors:
Ian Baine Pei Hui

Expert Rev Mol Diagn 2019 02 29;19(2):175-188. Epub 2019 Jan 29.

a Department of Pathology , Yale University School of Medicine , New Haven , CT , USA.

Introduction: Among various human tissue identity testing platforms, short tandem repeat (STR) genotyping has emerged as the most powerful and cost-effective method. Beyond forensic applications, tissue identity testing has become increasingly important in modern medical practice, in areas such as diagnostic pathology. Areas covered: A brief overview of various molecular/genetic techniques for identity testing is provided. Read More

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http://dx.doi.org/10.1080/14737159.2019.1568874DOI Listing
February 2019
3 Reads

CRISPR-based RNA editing: diagnostic applications and therapeutic options.

Expert Rev Mol Diagn 2019 02 21;19(2):83-88. Epub 2019 Jan 21.

e Department of Experimental Therapeutics , The University of Texas MD Anderson Cancer Center , Houston , TX.

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http://dx.doi.org/10.1080/14737159.2019.1568242DOI Listing
February 2019
5 Reads

Profile of the SeptiCyte™ LAB gene expression assay to diagnose infection in critically ill patients.

Expert Rev Mol Diagn 2019 02 5;19(2):95-108. Epub 2019 Feb 5.

b Department of Intensive Care , University Medical Center Utrecht , Utrecht , The Netherlands.

Sepsis is a severe and frequently occurring clinical syndrome, caused by the inflammatory response to infections. Recent studies on the human transcriptome during sepsis have yielded several gene-expression assays that might assist physicians during clinical assessment of patients suspected of sepsis. SeptiCyte™ LAB (Immunexpress, Seattle, WA) is the first gene expression assay that was cleared by the FDA in the United States to distinguish infectious from non-infectious causes of systemic inflammation in critically ill patients. Read More

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http://dx.doi.org/10.1080/14737159.2019.1567333DOI Listing
February 2019
1 Read

Should next-generation sequencing tests be performed on all cancer patients?

Expert Rev Mol Diagn 2019 02 9;19(2):89-93. Epub 2019 Jan 9.

a Sarah Cannon Research Institute , Nashville , Tennessee , USA.

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http://dx.doi.org/10.1080/14737159.2019.1564043DOI Listing
February 2019
3 Reads

Screening of fetal chromosomal aneuploidy diseases using noninvasive prenatal testing in twin pregnancies.

Expert Rev Mol Diagn 2019 02 2;19(2):189-196. Epub 2019 Jan 2.

a Department of Obestetrics and Gybecology , Shengjing Hospital of China Medical University, Liaoning Centre for Prenatal Diagnosis , Shenyang , China.

Objectives: This study was aimed to report the clinical characteristics of fetal chromosomal aneuploidy diseases using noninvasive prenatal testing (NIPT) in twin pregnancies and analyze the results in terms of chorionicity, conception, and fetal fraction.

Methods: A total of 1160 women with twin pregnancies were recruited from 1 October 2015, to 1 August 2017. Next-generation sequencing technology was used to detect fetal aneuploidies, such as trisomy 21, trisomy 18, trisomy 13 and trisomy X. Read More

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http://dx.doi.org/10.1080/14737159.2019.1562906DOI Listing
February 2019
5 Reads

Advances in prognostic biomarkers for esophageal cancer.

Expert Rev Mol Diagn 2019 02 30;19(2):109-119. Epub 2018 Dec 30.

a State Key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases, and Xijing Hospital of Digestive Diseases , Air Force Military Medical University , Xi'an , China.

Introduction: Esophageal cancer (EC) is one of the deadliest neoplasms in Asian countries. The high mortality rate and low survival rate of this disease are the major challenges for patients. Therefore, exploring novel biomarkers for prognosis analysis is still an important task for cancer research. Read More

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http://dx.doi.org/10.1080/14737159.2019.1563485DOI Listing
February 2019
3 Reads

What is the potential of p53 isoforms as a predictive biomarker in the treatment of cancer?

Expert Rev Mol Diagn 2019 02 7;19(2):149-159. Epub 2019 Jan 7.

b School of Medicine , University of Dundee, Dundee Cancer Centre , Dundee , Scotland.

Introduction: For decades, p53 was researched as a single protein with alterations described as mutants. The discovery of 12 human p53 isoforms expressed from 9 transcripts changed this perception, eloquently explaining the numerous roles p53 plays, including apoptosis, senescence, and regeneration. Area covered: Here, we summarise the p53 isoforms and their relevance to cancer to establish an understanding and theorise on potential applications of the isoforms in clinical practice. Read More

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http://dx.doi.org/10.1080/14737159.2019.1563484DOI Listing
February 2019
2 Reads

Breath analysis in respiratory diseases: state-of-the-art and future perspectives.

Expert Rev Mol Diagn 2019 01 21;19(1):47-61. Epub 2018 Dec 21.

a Unit of Geriatrics , Campus Bio-Medico University, Rome, Italy.

Introduction: The vast majority of respiratory diseases are associated with the production of volatile organic compounds (VOCs), the analysis of which might improve our knowledge about these disorders and their clinical management. The aim of this narrative review is to provide a comprehensive summary of current evidence supporting the application of breath analysis in the field of respiratory diseases, as well as suggesting potential applications available in the near future. Areas covered: A computerized literature search was performed to identify relevant articles reporting original data on the clinical use of breath analysis in respiratory diseases. Read More

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http://dx.doi.org/10.1080/14737159.2019.1559052DOI Listing
January 2019
1 Read

Advances in multiplex nucleic acid diagnostics for blood-borne pathogens: promises and pitfalls - an update.

Expert Rev Mol Diagn 2019 01 21;19(1):15-25. Epub 2018 Dec 21.

a Center for Biologics Evaluation and Research , US FDA , Silver Spring , MD , USA.

Introduction: Multiplex nucleic acid diagnostics for blood-borne pathogens have moved closer to clinical application in the two years since we first reviewed this topic. Areas covered: A new emphasis on detecting pathogens directly in a blood sample without culture, coupling PCR amplification to microfluidic devices and higher multiplexing in isothermal amplification are some of the advances. A wholly new approach of correlating host gene expression response with specific infectious agents opens another opportunity for multiplex detection. Read More

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http://dx.doi.org/10.1080/14737159.2019.1559055DOI Listing
January 2019
1 Read

Evolving role of human papillomavirus as a clinically significant biomarker in head and neck squamous cell carcinoma.

Expert Rev Mol Diagn 2018 Dec 21:1-8. Epub 2018 Dec 21.

b Department of Anatomic Pathology , Moffitt Cancer Center , Tampa , FL , USA.

Introduction: The majority of squamous cell carcinoma (SCC) of the oropharynx, one of the sites within the head and neck region, is now associated with high-risk human papillomavirus (HPV) in North America. Several modalities are available to determine the HPV status, however, the understanding of each assay in its application and limitations is essential for accurate interpretation and appropriate utilization of results in management of these patients. Areas covered: This expert review will cover the role of HPV in head and neck squamous cell carcinoma (HNSCC), indications for HPV testing, HPV detection methods in tumors, saliva and serum, and exploiting HPV status as a prognostic biomarker of clinical outcome in HNSCC. Read More

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http://dx.doi.org/10.1080/14737159.2019.1559056DOI Listing
December 2018
1 Read

A review of the AtomoRapid HIV self-testing device: an acceptable and easy alternative to facilitate HIV testing.

Expert Rev Mol Diagn 2018 Dec 20. Epub 2018 Dec 20.

a The Desmond Tutu HIV Centre , University of Cape Town , RSA.

HIV testing is the gateway to both HIV prevention and treatment, and increased HIV testing and linkage to services is vital for an effective HIV response. HIV testing has progressed significantly from a lengthy laboratory process conducted by specialist medical staff, to rapid point of care testing performed by trained lay staff. Despite HIV testing services being widely available, testing rates remain suboptimal among young people and men. Read More

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http://dx.doi.org/10.1080/14737159.2019.1561286DOI Listing
December 2018
1 Read

Recent advances in histone modification and histone modifying enzyme assays.

Expert Rev Mol Diagn 2018 Dec 19:1-10. Epub 2018 Dec 19.

a College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals , Shandong Normal University , Jinan , China.

Introduction: Histone modifying enzymes (HMEs)-catalyzed histone modifications are important epigenetic markers that play critical roles in the regulation of a variety of cellular functions, especially the regulation of gene expression. The aberrant histone modifying enzyme activity and the abnormal histone modification level are closely associated with various human diseases including cancers, making them become the promising and attractive disease biomarkers. Consequently, the development of efficient assays for accurate and sensitive detection of histone modifications and HMEs are crucial for disease diagnosis. Read More

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http://dx.doi.org/10.1080/14737159.2019.1559053DOI Listing
December 2018
2 Reads

Systematic review on recent potential biomarkers of chronic obstructive pulmonary disease.

Expert Rev Mol Diagn 2018 Dec 18:1-9. Epub 2018 Dec 18.

d Department of Biotechnology , Biomedical Research Foundation, Academy of Athens , Athens , Greece.

Introduction: Chronic obstructive pulmonary disease (COPD) is one of the leading causes of death worldwide and associated with decreased lung function and inflammation. The heterogeneity of COPD and its molecular and clinical features hinder efficient patient stratification and introduction of personalized therapeutic approaches. The available clinical tools do not efficiently predict the progression and exacerbations of the disease. Read More

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http://dx.doi.org/10.1080/14737159.2018.1559054DOI Listing
December 2018
12 Reads

Assay challenges (and opportunities) with non-factor VIII therapies for Hemophilia A.

Expert Rev Mol Diagn 2019 01 24;19(1):1-3. Epub 2018 Dec 24.

a Rare Blood Disorders Research , Bioverativ, A Sanofi Company , Waltham , MA , USA.

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http://dx.doi.org/10.1080/14737159.2019.1554436DOI Listing
January 2019
10 Reads

Label-free plasmonic biosensors for point-of-care diagnostics: a review.

Expert Rev Mol Diagn 2018 Dec 4. Epub 2018 Dec 4.

a Nanobiosensors and Bioanalytical Applications Group , Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, BIST and CIBER-BBN , Campus UAB, Ed-ICN2, 08193 Bellaterra , Barcelona , Spain .

Introduction: Optical biosensors and particularly those based on nanoplasmonics technology have emerged in recent decades as a potential solution for disease diagnostics and therapy follow-up at the point-of-care. These biosensor platforms could overcome some of the challenges faced in conventional diagnosis techniques offering label-free assays with immediate results and employing small and user-friendly devices. Areas covered: In this review, we will provide a critical overview of the recent advances in the development of nanoplasmonic biosensors for point-of-care diagnostics. Read More

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https://www.tandfonline.com/doi/full/10.1080/14737159.2019.1
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http://dx.doi.org/10.1080/14737159.2019.1554435DOI Listing
December 2018
9 Reads

Plasma DNA for early cancer detection - opportunities and challenges.

Expert Rev Mol Diagn 2018 Dec 4. Epub 2018 Dec 4.

a Department of Chemical Pathology , The Chinese University of Hong Kong, Prince of Wales Hospital , Hong Kong SAR , China.

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http://dx.doi.org/10.1080/14737159.2019.1555476DOI Listing
December 2018
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Upper urinary tract urothelial carcinoma and its variants: transition from morphology to personalized molecular characterization in diagnosis, prognosis, and therapy.

Expert Rev Mol Diagn 2018 12 27;18(12):1021-1028. Epub 2018 Nov 27.

a Section of Pathological Anatomy, School of Medicine, United Hospitals , Polytechnic University of the Marche Region , Ancona , Italy.

Introduction: Upper tract urothelial carcinoma (UTUC) accounts for 5% of urothelial carcinomas (UCs), the estimated annual incidence being 1-2 cases per 100,000 inhabitants. The aim of this review was to summarize the main morphologic features of UTUC as well as the current status and future role of its molecular characterization in diagnosis, prognosis, and therapy. Areas covered: Several studies on the UTUCs have shown the diagnostic, prognostic, and therapeutic significance of standard pathological features, such as tumor stage, grade, and lymph node metastasis. Read More

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https://www.tandfonline.com/doi/full/10.1080/14737159.2018.1
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http://dx.doi.org/10.1080/14737159.2018.1549490DOI Listing
December 2018
24 Reads

The clinical use of IDH1 and IDH2 mutations in gliomas.

Expert Rev Mol Diagn 2018 Nov 14:1-11. Epub 2018 Nov 14.

d Sorbonne Universités , Paris , France.

Introduction: Mutations in the genes isocitrate dehydrogenase (IDH) 1 and 2 have been reported in a limited number of tumors. In gliomas, IDH mutations are primarily detected in WHO grade II-III tumors and represent a major biomarker with diagnostic, prognostic, and predictive implications. The recent development of IDH inhibitors and vaccines suggests that the IDH mutation is also an appealing target for therapy. Read More

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https://www.tandfonline.com/doi/full/10.1080/14737159.2018.1
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http://dx.doi.org/10.1080/14737159.2018.1548935DOI Listing
November 2018
43 Reads

An updated view on the origin and use of angiogenic biomarkers for preeclampsia.

Expert Rev Mol Diagn 2018 Nov 9:1-9. Epub 2018 Nov 9.

a Division of Cell Biology, Histology and Embryology , Gottfried Schatz Research Center, Medical University of Graz , Graz , Austria.

Introduction: The last decade has seen massive efforts towards the identification and the potential use of predictive biomarkers for the pregnancy pathology preeclampsia. The angiogenic factors sFlt-1 and placental growth factor (PGF) have been in focus and have been massively supported. Areas covered: This review describes preeclampsia and intra-uterine growth restriction (IUGR), focusing on sFlt-1 and PGF, their sources during and outside pregnancy and the application of these markers in diseases outside pregnancy. Read More

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http://dx.doi.org/10.1080/14737159.2018.1546579DOI Listing
November 2018
2 Reads

Tumor-specific methylations in circulating cell-free DNA as clinically applicable markers with potential to substitute mutational analyses.

Expert Rev Mol Diagn 2018 Nov 9:1-9. Epub 2018 Nov 9.

a Department of Biochemistry and Immunology , Vejle Hospital , Vejle , Denmark.

Introduction: Analysis of circulating tumor DNA is a promising approach to guide the treatment of cancer patients but despite large efforts it has not been broadly applied in the clinic. Technical obstacles and lack of standardization have hampered the process and it has proved challenging to make highly sensitive analyses available for all patients. Research has focused on the use of somatic mutations but because of large mutational diversity among patients the setup becomes unmanageable for daily routine use. Read More

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https://www.tandfonline.com/doi/full/10.1080/14737159.2018.1
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http://dx.doi.org/10.1080/14737159.2018.1545576DOI Listing
November 2018
14 Reads

The potential of tumor-derived exosomes for noninvasive cancer monitoring: an update.

Expert Rev Mol Diagn 2018 Nov 8:1-12. Epub 2018 Nov 8.

a Departments of Pathology, Immunology and Otolaryngology , University of Pittsburgh School of Medicine and UPMC Hillman Cancer Center , Pittsburgh , PA , USA.

Introduction: Liquid biopsy platforms are being actively developed in the biomarker field. Extracellular vesicles (EVs), especially the tumor-derived exosome (TEX) subsets of EVs, represent a platform that allows for molecular and genetic profiling of parent tumor cells. TEX are ubiquitous in body fluids of cancer patients and are promising clinically relevant surrogates of cancer cells. Read More

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https://www.tandfonline.com/doi/full/10.1080/14737159.2018.1
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http://dx.doi.org/10.1080/14737159.2018.1544494DOI Listing
November 2018
15 Reads

Reproductive autonomy in expanded carrier screening: more than meets the eye?

Expert Rev Mol Diagn 2018 Nov 5:1-2. Epub 2018 Nov 5.

a Centre for Biomedical Ethics and Law, Department of Public Health and Primary Care , University of Leuven , Leuven , Belgium.

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https://www.tandfonline.com/doi/full/10.1080/14737159.2018.1
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http://dx.doi.org/10.1080/14737159.2018.1544496DOI Listing
November 2018
15 Reads

The microbiome of pancreatic cancer: from molecular diagnostics to new therapeutic approaches to overcome chemoresistance caused by metabolic inactivation of gemcitabine.

Expert Rev Mol Diagn 2018 Nov 5:1-5. Epub 2018 Nov 5.

b Department of Medical Oncology, Cancer Center Amsterdam, Amsterdam UMC , VU University Amsterdam , Amsterdam , The Netherlands.

Introduction: Pancreatic cancer is a complex disease, with an extremely poor response to chemotherapy. Emerging evidence indicates that the tumor microenvironment (TME) might play an important role in mediating chemoresistance. Areas covered: The evaluated study by Geller and collaborators describes several bacterial species within pancreatic tumor tissues and TME and investigated their roles in gemcitabine chemoresistance. Read More

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https://www.tandfonline.com/doi/full/10.1080/14737159.2018.1
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http://dx.doi.org/10.1080/14737159.2018.1544495DOI Listing
November 2018
18 Reads

PCR detection of Mycobacterium ulcerans-significance for clinical practice and epidemiology.

Expert Rev Mol Diagn 2018 Nov 1:1-12. Epub 2018 Nov 1.

a Division of Infectious Diseases and Tropical Medicine , University Hospital, Ludwigs-Maximilians-University , Munich , Germany.

Introduction: Buruli ulcer (BU) is a neglected disease which has been reported from mostly impoverished, remote rural areas from 35 countries worldwide. BU affects skin, subcutaneous tissue, and bones, and may cause massive tissue destruction and life-long disabilities if not diagnosed and treated early. Without laboratory confirmation diagnostic and treatment errors may occur. Read More

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http://dx.doi.org/10.1080/14737159.2018.1543592DOI Listing
November 2018
8 Reads

Profile of the VERSANT HCV genotype 2.0 assay.

Expert Rev Mol Diagn 2018 12 2;18(12):995-1004. Epub 2018 Nov 2.

a Peking University People's Hospital, Peking University Hepatology Institute , Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Diseases , Beijing , China.

Introduction: Hepatitis C virus (HCV) is divided into 7 genotypes and 67 subtypes. HCV genotype studies reflect the viral transmission patterns as well as human migration routes. In a clinical setting, HCV genotype is a baseline predictor for the sustained virological response (SVR) in chronic hepatitis C patients treated with peginterferon or some direct acting antivirals (DAAs). Read More

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https://www.tandfonline.com/doi/full/10.1080/14737159.2018.1
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http://dx.doi.org/10.1080/14737159.2018.1541740DOI Listing
December 2018
22 Reads