1,696 results match your criteria Expert Review of Molecular Diagnostics [Journal]


Early detection and monitoring of cancer in liquid biopsy: advances and challenges.

Expert Rev Mol Diagn 2019 Feb 13. Epub 2019 Feb 13.

b Department of Clinical Oncology , Queen Elizabeth Hospital , Hong Kong.

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http://dx.doi.org/10.1080/14737159.2019.1583104DOI Listing
February 2019
3.516 Impact Factor

Prognostic significance of gamma-glutamyl transferase in patients with metastatic non-small cell lung cancer.

Expert Rev Mol Diagn 2019 Feb 6:1-6. Epub 2019 Feb 6.

b Department of Medical Oncology , Gazi University Faculty of Medicine , Ankara , Turkey.

Background: The prognostic significance of serum gamma-glutamyl transferase (GGT) level at diagnosis in patients with metastatic non-small lung cancer (NSCLC) is not clear. We aimed to assess the relationship between serum GGT level and overall survival (OS) and progression-free survival (PFS) in this patient population.

Methods: Data of patients with metastatic NSCLC who were admitted to the medical oncology clinic of our hospital during April 2013-December 2017 were retrospectively analyzed. Read More

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http://dx.doi.org/10.1080/14737159.2019.1579644DOI Listing
February 2019
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The expanding use of matrix-assisted laser desorption/ionization-time of flight mass spectroscopy in the diagnosis of patients with mycotic diseases.

Expert Rev Mol Diagn 2019 Feb 5:1-8. Epub 2019 Feb 5.

b Division of General Internal Medicine , Weill Cornell Medicine of Cornell University , New York , NY , USA.

Introduction: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a powerful new tool to identify human fungal pathogens and has radically altered the diagnostic mycology workflow at many medical centers around the world. Areas covered: While most experience is with the identification of yeasts, including species of Candida and Cryptococcus, there is ongoing work investigating the role of MALDI-TOF MS to detect molds, including species of Aspergillus, Fusarium, Scedosporium, and Mucormyctes as well as thermally dimorphic fungi. Expert commentary: In this paper, we review the current knowledge about this important new platform and examine how its expanding use may impact molecular diagnostics and patient care in the years ahead. Read More

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http://dx.doi.org/10.1080/14737159.2019.1574572DOI Listing
February 2019
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From next-generation sequencing to targeted treatment of non-acquired epilepsies.

Expert Rev Mol Diagn 2019 Jan 19:1-12. Epub 2019 Jan 19.

a Department of Epilepsy Genetics and Precision Medicine , The Danish Epilepsy Centre , Dianalund , Denmark.

Introduction: Within the last decade, next-generation sequencing (NGS) has resulted in remarkable advances in the field of epilepsy genetics. NGS has become a routine part of the diagnostic workup in many countries. A workup that has led to higher diagnostic yields and insights into the underlying disease mechanisms. Read More

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http://dx.doi.org/10.1080/14737159.2019.1573144DOI Listing
January 2019
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The importance of pre- and post-test counseling for prenatal cell-free DNA screening for common fetal aneuploidies.

Expert Rev Mol Diagn 2019 Jan 18:1-15. Epub 2019 Jan 18.

a Department of Obstetrics and Gynecology , Fetomaternal Medical Center, Helsinki University and Helsinki University Hospital , Helsinki , Finland.

Introduction: Prenatal cell-free DNA screening for common fetal aneuploidies has rapidly changed the paradigm of prenatal care. Despite its advantages compared to conventional screening methods, its unexpectedly rapid implementation in clinical practice has generated several ethical and medical issues and misconceptions. Aggressive commercial marketing of cell-free DNA screening and media dissemination of misleading information have added confusion. Read More

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http://dx.doi.org/10.1080/14737159.2019.1571912DOI Listing
January 2019
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Soluble CD163 is a potential biomarker in systemic sclerosis.

Expert Rev Mol Diagn 2019 Jan 18:1-3. Epub 2019 Jan 18.

a Department of Dermatology, Faculty of Medicine, Institute of Medical, Pharmaceutical and Health Sciences , Kanazawa University , Kanazawa , Japan.

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http://dx.doi.org/10.1080/14737159.2019.1571911DOI Listing
January 2019

Loop-mediated isothermal amplification assay as a point-of-care diagnostic tool for Vibrio parahaemolyticus: recent developments and improvements.

Expert Rev Mol Diagn 2019 Jan 18:1-11. Epub 2019 Jan 18.

a Division of Infectious Diseases , Nitte University Centre for Science Education and Research , Mangaluru , India.

Introduction: A number of DNA-based diagnostic tools have been developed for the detection of Vibrio parahaemolyticus in seafood. However, the loop-mediated isothermal amplification (LAMP) has distinct advantages with regards to its simplicity, speed and the ease of performing without any need for sophisticated equipment. Over the last decade, LAMP has emerged as a potential tool for the detection of V. Read More

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http://dx.doi.org/10.1080/14737159.2019.1571913DOI Listing
January 2019
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Technical considerations for circulating tumor DNA detection in oncology.

Expert Rev Mol Diagn 2019 Feb 23;19(2):121-135. Epub 2019 Jan 23.

b Service de Biopathologie , Institut de Cancérologie de Lorraine, Université de Lorraine, CNRS UMR 7039 CRAN , Nancy , France.

Introduction: Blood draw or collection of other body fluids, known as 'liquid biopsies' are generally less invasive procedures than tumor biopsies. Cell-free tumor DNA (ctDNA) is widely evaluated in cancer for early detection, diagnosis, prognosis, therapy monitoring or determination of minimal residual disease. In body fluid samples, ctDNA can represent a small fraction of total cell-free DNA (cfDNA), requiring highly sensitive assays. Read More

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http://dx.doi.org/10.1080/14737159.2019.1568873DOI Listing
February 2019
5 Reads

Promoter methylation of galanin receptors as epigenetic biomarkers for head and neck squamous cell carcinomas.

Expert Rev Mol Diagn 2019 Feb 21;19(2):137-148. Epub 2019 Jan 21.

f Laboratory of Head and Neck Center Biology, Department of Otolaryngology, Head and Neck Surgery , The University of Michigan , Ann Arbor , MI , USA.

Introduction: While remarkable progress has been made in standard treatments for head and neck squamous cell carcinomas (HNSCCs), the long-term survival remains at an unsatisfactory 40-50%. To improve the survival rate, biomarkers for optimal treatment selection and prognostic prediction, as well as novel, low-toxicity treatment strategies, are required. Galanin receptor (GALR) 1 and GALR2 are well-studied tumor suppressors in HNSCCs. Read More

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http://dx.doi.org/10.1080/14737159.2019.1567334DOI Listing
February 2019

Emerging methodologies for pathogen identification in bloodstream infections: an update.

Expert Rev Mol Diagn 2019 Feb 21;19(2):161-173. Epub 2019 Jan 21.

b Aix Marseille Univ, IRD, AP-HM, SSA, VITROME , Marseille , France.

Introduction: Bloodstream infections (BSIs) remain a major public health burden worldwide, particularly in high-income countries as they are associated with a significant mortality rate. As early administration of appropriate antimicrobial therapy is a major prognostic factor, there remain unmet needs for shortening BSI diagnosis. Current blood cultures (BC) processing to identify pathogens involved in BSI is not compatible with such delays, although it remains the gold standard. Read More

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http://dx.doi.org/10.1080/14737159.2019.1568241DOI Listing
February 2019
5 Reads

Practical applications of DNA genotyping in diagnostic pathology.

Authors:
Ian Baine Pei Hui

Expert Rev Mol Diagn 2019 Feb 29;19(2):175-188. Epub 2019 Jan 29.

a Department of Pathology , Yale University School of Medicine , New Haven , CT , USA.

Introduction: Among various human tissue identity testing platforms, short tandem repeat (STR) genotyping has emerged as the most powerful and cost-effective method. Beyond forensic applications, tissue identity testing has become increasingly important in modern medical practice, in areas such as diagnostic pathology. Areas covered: A brief overview of various molecular/genetic techniques for identity testing is provided. Read More

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http://dx.doi.org/10.1080/14737159.2019.1568874DOI Listing
February 2019
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CRISPR-based RNA editing: diagnostic applications and therapeutic options.

Expert Rev Mol Diagn 2019 Feb 21;19(2):83-88. Epub 2019 Jan 21.

e Department of Experimental Therapeutics , The University of Texas MD Anderson Cancer Center , Houston , TX.

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http://dx.doi.org/10.1080/14737159.2019.1568242DOI Listing
February 2019
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Profile of the SeptiCyte™ LAB gene expression assay to diagnose infection in critically ill patients.

Expert Rev Mol Diagn 2019 Feb 5;19(2):95-108. Epub 2019 Feb 5.

b Department of Intensive Care , University Medical Center Utrecht , Utrecht , The Netherlands.

Sepsis is a severe and frequently occurring clinical syndrome, caused by the inflammatory response to infections. Recent studies on the human transcriptome during sepsis have yielded several gene-expression assays that might assist physicians during clinical assessment of patients suspected of sepsis. SeptiCyte™ LAB (Immunexpress, Seattle, WA) is the first gene expression assay that was cleared by the FDA in the United States to distinguish infectious from non-infectious causes of systemic inflammation in critically ill patients. Read More

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http://dx.doi.org/10.1080/14737159.2019.1567333DOI Listing
February 2019

Should next-generation sequencing tests be performed on all cancer patients?

Expert Rev Mol Diagn 2019 Feb 9;19(2):89-93. Epub 2019 Jan 9.

a Sarah Cannon Research Institute , Nashville , Tennessee , USA.

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http://dx.doi.org/10.1080/14737159.2019.1564043DOI Listing
February 2019
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Screening of fetal chromosomal aneuploidy diseases using noninvasive prenatal testing in twin pregnancies.

Expert Rev Mol Diagn 2019 Feb 2;19(2):189-196. Epub 2019 Jan 2.

a Department of Obestetrics and Gybecology , Shengjing Hospital of China Medical University, Liaoning Centre for Prenatal Diagnosis , Shenyang , China.

Objectives: This study was aimed to report the clinical characteristics of fetal chromosomal aneuploidy diseases using noninvasive prenatal testing (NIPT) in twin pregnancies and analyze the results in terms of chorionicity, conception, and fetal fraction.

Methods: A total of 1160 women with twin pregnancies were recruited from 1 October 2015, to 1 August 2017. Next-generation sequencing technology was used to detect fetal aneuploidies, such as trisomy 21, trisomy 18, trisomy 13 and trisomy X. Read More

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http://dx.doi.org/10.1080/14737159.2019.1562906DOI Listing
February 2019
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Advances in prognostic biomarkers for esophageal cancer.

Expert Rev Mol Diagn 2019 Feb 30;19(2):109-119. Epub 2018 Dec 30.

a State Key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases, and Xijing Hospital of Digestive Diseases , Air Force Military Medical University , Xi'an , China.

Introduction: Esophageal cancer (EC) is one of the deadliest neoplasms in Asian countries. The high mortality rate and low survival rate of this disease are the major challenges for patients. Therefore, exploring novel biomarkers for prognosis analysis is still an important task for cancer research. Read More

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http://dx.doi.org/10.1080/14737159.2019.1563485DOI Listing
February 2019

What is the potential of p53 isoforms as a predictive biomarker in the treatment of cancer?

Expert Rev Mol Diagn 2019 Feb 7;19(2):149-159. Epub 2019 Jan 7.

b School of Medicine , University of Dundee, Dundee Cancer Centre , Dundee , Scotland.

Introduction: For decades, p53 was researched as a single protein with alterations described as mutants. The discovery of 12 human p53 isoforms expressed from 9 transcripts changed this perception, eloquently explaining the numerous roles p53 plays, including apoptosis, senescence, and regeneration. Area covered: Here, we summarise the p53 isoforms and their relevance to cancer to establish an understanding and theorise on potential applications of the isoforms in clinical practice. Read More

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http://dx.doi.org/10.1080/14737159.2019.1563484DOI Listing
February 2019

Breath analysis in respiratory diseases: state-of-the-art and future perspectives.

Expert Rev Mol Diagn 2019 Jan 21;19(1):47-61. Epub 2018 Dec 21.

a Unit of Geriatrics , Campus Bio-Medico University, Rome, Italy.

Introduction: The vast majority of respiratory diseases are associated with the production of volatile organic compounds (VOCs), the analysis of which might improve our knowledge about these disorders and their clinical management. The aim of this narrative review is to provide a comprehensive summary of current evidence supporting the application of breath analysis in the field of respiratory diseases, as well as suggesting potential applications available in the near future. Areas covered: A computerized literature search was performed to identify relevant articles reporting original data on the clinical use of breath analysis in respiratory diseases. Read More

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http://dx.doi.org/10.1080/14737159.2019.1559052DOI Listing
January 2019

Advances in multiplex nucleic acid diagnostics for blood-borne pathogens: promises and pitfalls - an update.

Expert Rev Mol Diagn 2019 Jan 21;19(1):15-25. Epub 2018 Dec 21.

a Center for Biologics Evaluation and Research , US FDA , Silver Spring , MD , USA.

Introduction: Multiplex nucleic acid diagnostics for blood-borne pathogens have moved closer to clinical application in the two years since we first reviewed this topic. Areas covered: A new emphasis on detecting pathogens directly in a blood sample without culture, coupling PCR amplification to microfluidic devices and higher multiplexing in isothermal amplification are some of the advances. A wholly new approach of correlating host gene expression response with specific infectious agents opens another opportunity for multiplex detection. Read More

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http://dx.doi.org/10.1080/14737159.2019.1559055DOI Listing
January 2019

Evolving role of human papillomavirus as a clinically significant biomarker in head and neck squamous cell carcinoma.

Expert Rev Mol Diagn 2018 Dec 21:1-8. Epub 2018 Dec 21.

b Department of Anatomic Pathology , Moffitt Cancer Center , Tampa , FL , USA.

Introduction: The majority of squamous cell carcinoma (SCC) of the oropharynx, one of the sites within the head and neck region, is now associated with high-risk human papillomavirus (HPV) in North America. Several modalities are available to determine the HPV status, however, the understanding of each assay in its application and limitations is essential for accurate interpretation and appropriate utilization of results in management of these patients. Areas covered: This expert review will cover the role of HPV in head and neck squamous cell carcinoma (HNSCC), indications for HPV testing, HPV detection methods in tumors, saliva and serum, and exploiting HPV status as a prognostic biomarker of clinical outcome in HNSCC. Read More

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http://dx.doi.org/10.1080/14737159.2019.1559056DOI Listing
December 2018

A review of the AtomoRapid HIV self-testing device: an acceptable and easy alternative to facilitate HIV testing.

Expert Rev Mol Diagn 2018 Dec 20. Epub 2018 Dec 20.

a The Desmond Tutu HIV Centre , University of Cape Town , RSA.

HIV testing is the gateway to both HIV prevention and treatment, and increased HIV testing and linkage to services is vital for an effective HIV response. HIV testing has progressed significantly from a lengthy laboratory process conducted by specialist medical staff, to rapid point of care testing performed by trained lay staff. Despite HIV testing services being widely available, testing rates remain suboptimal among young people and men. Read More

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http://dx.doi.org/10.1080/14737159.2019.1561286DOI Listing
December 2018

Recent advances in histone modification and histone modifying enzyme assays.

Expert Rev Mol Diagn 2018 Dec 19:1-10. Epub 2018 Dec 19.

a College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals , Shandong Normal University , Jinan , China.

Introduction: Histone modifying enzymes (HMEs)-catalyzed histone modifications are important epigenetic markers that play critical roles in the regulation of a variety of cellular functions, especially the regulation of gene expression. The aberrant histone modifying enzyme activity and the abnormal histone modification level are closely associated with various human diseases including cancers, making them become the promising and attractive disease biomarkers. Consequently, the development of efficient assays for accurate and sensitive detection of histone modifications and HMEs are crucial for disease diagnosis. Read More

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http://dx.doi.org/10.1080/14737159.2019.1559053DOI Listing
December 2018
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Systematic review on recent potential biomarkers of chronic obstructive pulmonary disease.

Expert Rev Mol Diagn 2018 Dec 18:1-9. Epub 2018 Dec 18.

d Department of Biotechnology , Biomedical Research Foundation, Academy of Athens , Athens , Greece.

Introduction: Chronic obstructive pulmonary disease (COPD) is one of the leading causes of death worldwide and associated with decreased lung function and inflammation. The heterogeneity of COPD and its molecular and clinical features hinder efficient patient stratification and introduction of personalized therapeutic approaches. The available clinical tools do not efficiently predict the progression and exacerbations of the disease. Read More

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December 2018
9 Reads

Assay challenges (and opportunities) with non-factor VIII therapies for Hemophilia A.

Expert Rev Mol Diagn 2019 Jan 24;19(1):1-3. Epub 2018 Dec 24.

a Rare Blood Disorders Research , Bioverativ, A Sanofi Company , Waltham , MA , USA.

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http://dx.doi.org/10.1080/14737159.2019.1554436DOI Listing
January 2019
6 Reads

Label-free plasmonic biosensors for point-of-care diagnostics: a review.

Expert Rev Mol Diagn 2018 Dec 4. Epub 2018 Dec 4.

a Nanobiosensors and Bioanalytical Applications Group , Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, BIST and CIBER-BBN , Campus UAB, Ed-ICN2, 08193 Bellaterra , Barcelona , Spain .

Introduction: Optical biosensors and particularly those based on nanoplasmonics technology have emerged in recent decades as a potential solution for disease diagnostics and therapy follow-up at the point-of-care. These biosensor platforms could overcome some of the challenges faced in conventional diagnosis techniques offering label-free assays with immediate results and employing small and user-friendly devices. Areas covered: In this review, we will provide a critical overview of the recent advances in the development of nanoplasmonic biosensors for point-of-care diagnostics. Read More

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http://dx.doi.org/10.1080/14737159.2019.1554435DOI Listing
December 2018
4 Reads

Plasma DNA for early cancer detection - opportunities and challenges.

Expert Rev Mol Diagn 2018 Dec 4. Epub 2018 Dec 4.

a Department of Chemical Pathology , The Chinese University of Hong Kong, Prince of Wales Hospital , Hong Kong SAR , China.

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http://dx.doi.org/10.1080/14737159.2019.1555476DOI Listing
December 2018

Upper urinary tract urothelial carcinoma and its variants: transition from morphology to personalized molecular characterization in diagnosis, prognosis, and therapy.

Expert Rev Mol Diagn 2018 Dec 27;18(12):1021-1028. Epub 2018 Nov 27.

a Section of Pathological Anatomy, School of Medicine, United Hospitals , Polytechnic University of the Marche Region , Ancona , Italy.

Introduction: Upper tract urothelial carcinoma (UTUC) accounts for 5% of urothelial carcinomas (UCs), the estimated annual incidence being 1-2 cases per 100,000 inhabitants. The aim of this review was to summarize the main morphologic features of UTUC as well as the current status and future role of its molecular characterization in diagnosis, prognosis, and therapy. Areas covered: Several studies on the UTUCs have shown the diagnostic, prognostic, and therapeutic significance of standard pathological features, such as tumor stage, grade, and lymph node metastasis. Read More

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http://dx.doi.org/10.1080/14737159.2018.1549490DOI Listing
December 2018
14 Reads

The clinical use of IDH1 and IDH2 mutations in gliomas.

Expert Rev Mol Diagn 2018 Nov 14:1-11. Epub 2018 Nov 14.

d Sorbonne Universités , Paris , France.

Introduction: Mutations in the genes isocitrate dehydrogenase (IDH) 1 and 2 have been reported in a limited number of tumors. In gliomas, IDH mutations are primarily detected in WHO grade II-III tumors and represent a major biomarker with diagnostic, prognostic, and predictive implications. The recent development of IDH inhibitors and vaccines suggests that the IDH mutation is also an appealing target for therapy. Read More

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November 2018
29 Reads

An updated view on the origin and use of angiogenic biomarkers for preeclampsia.

Expert Rev Mol Diagn 2018 Nov 9:1-9. Epub 2018 Nov 9.

a Division of Cell Biology, Histology and Embryology , Gottfried Schatz Research Center, Medical University of Graz , Graz , Austria.

Introduction: The last decade has seen massive efforts towards the identification and the potential use of predictive biomarkers for the pregnancy pathology preeclampsia. The angiogenic factors sFlt-1 and placental growth factor (PGF) have been in focus and have been massively supported. Areas covered: This review describes preeclampsia and intra-uterine growth restriction (IUGR), focusing on sFlt-1 and PGF, their sources during and outside pregnancy and the application of these markers in diseases outside pregnancy. Read More

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http://dx.doi.org/10.1080/14737159.2018.1546579DOI Listing
November 2018
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Tumor-specific methylations in circulating cell-free DNA as clinically applicable markers with potential to substitute mutational analyses.

Expert Rev Mol Diagn 2018 Nov 9:1-9. Epub 2018 Nov 9.

a Department of Biochemistry and Immunology , Vejle Hospital , Vejle , Denmark.

Introduction: Analysis of circulating tumor DNA is a promising approach to guide the treatment of cancer patients but despite large efforts it has not been broadly applied in the clinic. Technical obstacles and lack of standardization have hampered the process and it has proved challenging to make highly sensitive analyses available for all patients. Research has focused on the use of somatic mutations but because of large mutational diversity among patients the setup becomes unmanageable for daily routine use. Read More

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November 2018
8 Reads

The potential of tumor-derived exosomes for noninvasive cancer monitoring: an update.

Expert Rev Mol Diagn 2018 Nov 8:1-12. Epub 2018 Nov 8.

a Departments of Pathology, Immunology and Otolaryngology , University of Pittsburgh School of Medicine and UPMC Hillman Cancer Center , Pittsburgh , PA , USA.

Introduction: Liquid biopsy platforms are being actively developed in the biomarker field. Extracellular vesicles (EVs), especially the tumor-derived exosome (TEX) subsets of EVs, represent a platform that allows for molecular and genetic profiling of parent tumor cells. TEX are ubiquitous in body fluids of cancer patients and are promising clinically relevant surrogates of cancer cells. Read More

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http://dx.doi.org/10.1080/14737159.2018.1544494DOI Listing
November 2018
9 Reads

Reproductive autonomy in expanded carrier screening: more than meets the eye?

Expert Rev Mol Diagn 2018 Nov 5:1-2. Epub 2018 Nov 5.

a Centre for Biomedical Ethics and Law, Department of Public Health and Primary Care , University of Leuven , Leuven , Belgium.

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November 2018
9 Reads

The microbiome of pancreatic cancer: from molecular diagnostics to new therapeutic approaches to overcome chemoresistance caused by metabolic inactivation of gemcitabine.

Expert Rev Mol Diagn 2018 Nov 5:1-5. Epub 2018 Nov 5.

b Department of Medical Oncology, Cancer Center Amsterdam, Amsterdam UMC , VU University Amsterdam , Amsterdam , The Netherlands.

Introduction: Pancreatic cancer is a complex disease, with an extremely poor response to chemotherapy. Emerging evidence indicates that the tumor microenvironment (TME) might play an important role in mediating chemoresistance. Areas covered: The evaluated study by Geller and collaborators describes several bacterial species within pancreatic tumor tissues and TME and investigated their roles in gemcitabine chemoresistance. Read More

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November 2018
8 Reads

PCR detection of Mycobacterium ulcerans-significance for clinical practice and epidemiology.

Expert Rev Mol Diagn 2018 Nov 1:1-12. Epub 2018 Nov 1.

a Division of Infectious Diseases and Tropical Medicine , University Hospital, Ludwigs-Maximilians-University , Munich , Germany.

Introduction: Buruli ulcer (BU) is a neglected disease which has been reported from mostly impoverished, remote rural areas from 35 countries worldwide. BU affects skin, subcutaneous tissue, and bones, and may cause massive tissue destruction and life-long disabilities if not diagnosed and treated early. Without laboratory confirmation diagnostic and treatment errors may occur. Read More

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http://dx.doi.org/10.1080/14737159.2018.1543592DOI Listing
November 2018
2 Reads

Profile of the VERSANT HCV genotype 2.0 assay.

Expert Rev Mol Diagn 2018 Dec 2;18(12):995-1004. Epub 2018 Nov 2.

a Peking University People's Hospital, Peking University Hepatology Institute , Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Diseases , Beijing , China.

Introduction: Hepatitis C virus (HCV) is divided into 7 genotypes and 67 subtypes. HCV genotype studies reflect the viral transmission patterns as well as human migration routes. In a clinical setting, HCV genotype is a baseline predictor for the sustained virological response (SVR) in chronic hepatitis C patients treated with peginterferon or some direct acting antivirals (DAAs). Read More

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http://dx.doi.org/10.1080/14737159.2018.1541740DOI Listing
December 2018
13 Reads

Biomarkers for Barrett's esophagus - a contemporary review.

Expert Rev Mol Diagn 2018 Nov 1;18(11):939-946. Epub 2018 Nov 1.

b Department of Pathology, Harvard Medical School , Brigham and Women's Hospital , Boston , MA.

Introduction: Esophageal adenocarcinoma (EAC) has a poor 5-year survival rate (10%-18%), and incidence has increased dramatically in the past three decades. Barrett's esophagus (BE) is the precursor lesion to EAC and is the replacement of the normally squamous lined esophagus with columnar cells that develop an intestinal phenotype characterized by the presence of goblet cells. Given the known precursor state, EAC is amenable to screening and surveillance strategies (analogous to colon cancer). Read More

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http://dx.doi.org/10.1080/14737159.2018.1538793DOI Listing
November 2018
12 Reads

Clinical utility of microRNAs in renal cell carcinoma: current evidence and future perspectives.

Expert Rev Mol Diagn 2018 Nov 26;18(11):981-991. Epub 2018 Oct 26.

a Department of Biochemistry and Molecular Biology , National and Kapodistrian University of Athens , Athens , Greece.

Introduction: MicroRNAs (miRNAs) are small, non-coding RNA molecules that epigenetically regulate gene expression. Recently, it has been shown that miRNAs are heavily involved in renal carcinogenesis by controlling the expression of proteins related to renal cell carcinoma (RCC) and, thus, the activity of key signaling pathways involved in pivotal cell processes. Areas covered: This review focuses on the role of miRNAs as emerging biomarkers in RCC. Read More

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http://dx.doi.org/10.1080/14737159.2018.1539668DOI Listing
November 2018
4 Reads

miRNA and long non-coding RNA: molecular function and clinical value in breast and ovarian cancers.

Expert Rev Mol Diagn 2018 Nov 29;18(11):963-979. Epub 2018 Oct 29.

a Department of Biochemistry and Molecular Biology, Faculty of Biology , National and Kapodistrian University of Athens , Athens , Greece.

Introduction: The elucidation of tumor molecular hallmarks and the identification of novel molecular markers are of first translational priority in breast and ovarian cancer research, aiming to support personalized disease treatment and monitoring decisions. Recent high-throughput studies have revealed that ~ 80% of the genome is transcribed into RNAs without protein-coding potential, namely non-coding RNAs (ncRNAs), challenging the concept of 'junk DNA'. Undoubtedly, microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) represent the best-studied family classes, emerging as the most powerful gene-expression regulators at epigenetic, transcriptional and post-transcriptional levels. Read More

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November 2018

Genetic testing for high-grade osteosarcoma: a guide for future tailored treatments?

Expert Rev Mol Diagn 2018 Nov 19;18(11):947-961. Epub 2018 Oct 19.

a Laboratory of Experimental Oncology , IRCCS Istituto Ortopedico Rizzoli , Bologna , Italy.

Introduction: Genetic characterization of osteosarcoma has evolved during the last decade, thanks to the integrated application of conventional and new candidate-driven and genome-wide technologies. Areas covered: This review provides an overview of the state of art in genetic testing applied to osteosarcoma, with particular regard to novel candidate genetic biomarkers that can be analyzed in tumor tissue and blood samples, which might be used to predict toxicity and prognosis, detect disease relapse, and improve patients' selection criteria for tailoring treatment. Expert commentary: Genetic testing based on modern technologies is expected to indicate new osteosarcoma-related prognostic markers and driver genes, which may highlight novel therapeutic targets and patients stratification biomarkers. Read More

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http://dx.doi.org/10.1080/14737159.2018.1535903DOI Listing
November 2018
12 Reads

We have the technology, but should we build the test?

Expert Rev Mol Diagn 2018 Nov 17;18(11):917-919. Epub 2018 Oct 17.

a University of Alabama at Birmingham , School of Medicine , Birmingham , AL , USA.

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November 2018

Prognostic utility of circulating nucleic acids in acute brain injuries.

Expert Rev Mol Diagn 2018 Nov 22;18(11):925-938. Epub 2018 Oct 22.

a School of Medicine , Lutheran University of Brazil , Canoas , RS , Brazil.

Introduction: Acute brain injuries represent major causes of morbidity and mortality worldwide. Nevertheless, therapeutic options are centered mainly on supportive care, and accurate prognosis prediction following traumatic brain injury (TBI) or stroke remains a challenge in clinical settings. Areas covered: Circulating DNA and RNA have shown potential as predictive molecules in acute brain injuries. Read More

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http://dx.doi.org/10.1080/14737159.2018.1535904DOI Listing
November 2018
4 Reads

Biomarkers for acute radiation syndrome: challenges for developing radiation countermeasures following animal rule.

Expert Rev Mol Diagn 2018 Nov 11;18(11):921-924. Epub 2018 Oct 11.

c Department of Anatomy, Physiology and Genetics, F. Edward Hébert School of Medicine , Uniformed Services University of the Health Sciences , Bethesda , MD USA.

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November 2018
1 Read
3.516 Impact Factor

Recent advances in molecular testing to improve early diagnosis in children with mucopolysaccharidoses.

Expert Rev Mol Diagn 2018 10 26;18(10):855-866. Epub 2018 Sep 26.

a Medical Genetics Service , HCPA , Porto Alegre , RS , Brazil.

Introduction: The mucopolysaccharidoses (MPS) are a group of lysosomal storage disorders with high phenotypic and genotypic heterogeneity, making precise diagnosis challenging. Although enzyme activity assay is considered the gold standard for the diagnosis of these disorders, molecular testing can greatly refine this task. New methods for rapid detection of variants are useful to reduce the 'diagnostic odyssey' faced by patients and their family, to lead to appropriate genetic counseling and to select the most appropriate therapy for each case. Read More

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October 2018

The clinical implementation of copy number detection in the age of next-generation sequencing.

Expert Rev Mol Diagn 2018 10 27;18(10):907-915. Epub 2018 Sep 27.

a Princess Máxima Center for Pediatric Oncology , Utrecht , Netherlands.

Introduction: The role of copy number variants (CNVs) in disease is now well established. In parallel NGS technologies, such as long-read technologies, there is continual development and data analysis methods continue to be refined. Clinical exome sequencing data is now a reality for many diagnostic laboratories in both congenital genetics and oncology. Read More

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October 2018
20 Reads

Effective early detection of oral cancer using a simple and inexpensive point of care device in oral rinses.

Expert Rev Mol Diagn 2018 10 26;18(10):837-844. Epub 2018 Sep 26.

b Department of Pathology , Icahn School of Medicine at Mount Sinai , New York , NY , USA.

Introduction: Head and neck cancer remains a challenging disease that is increasing in incidence with the majority of patients diagnosed at an advanced stage where 5-year survival is approximately 50%. Current approaches including oral-brush biopsies, fluorescence-based technologies,  and salivary molecular profiling have demonstrated some success; however, cost, ease of use, and accuracy remain limiting factors. Areas covered: This is a profile of a novel, easy to use oral rinse point-of-care (POC) test to aid in the diagnosis of oral and oropharyngeal cancer. Read More

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http://dx.doi.org/10.1080/14737159.2018.1523008DOI Listing
October 2018
14 Reads

Recent advances in the molecular diagnosis of mucormycosis.

Expert Rev Mol Diagn 2018 10 19;18(10):845-854. Epub 2018 Sep 19.

b Department of Infectious Diseases, Infection Control and Employee Health , The University of Texas MD Anderson Cancer Center , Houston , TX , USA.

Introduction: Fungal infection burden related to Mucorales has been on the rise with significant associated morbidity and mortality. The major obstacle in the management has been lack of a non-invasive rapid and a reliable diagnostic test. Developing a culture-independent biomarker for the early diagnosis of mucormycosis is a major unmet need in modern mycology. Read More

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http://dx.doi.org/10.1080/14737159.2018.1522250DOI Listing
October 2018
13 Reads

Circulating tumor cells as biomarkers in head and neck cancer: recent advances and future outlook.

Expert Rev Mol Diagn 2018 10 21;18(10):897-906. Epub 2018 Sep 21.

a Department of Radiooncology and Radiotherapy , Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health , Berlin , Germany.

Introduction: Assessment of circulating tumor cells (CTCs) in peripheral blood from solid cancer patients including head and neck squamous cell carcinoma (HNSCC) has proven useful for detection of subclinical disease that otherwise remains invisible for current staging techniques. Based on large cohort studies, diagnostic tests for enumeration of CTCs have been developed, which can be used for tumor staging, prognosis, treatment monitoring, and post-treatment surveillance. Areas covered: Here, we briefly summarize the history of CTC discovery. Read More

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October 2018
7 Reads

Tissue microarrays - translational biomarker research in the fast lane.

Expert Rev Mol Diagn 2018 10 13;18(10):833-835. Epub 2018 Sep 13.

b Center for CNS Tumors, Comprehensive Cancer Center Tuebingen-Stuttgart , University Hospital Tuebingen, Eberhard-Karls-University Tuebingen , Tuebingen , Germany.

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October 2018
1 Read

Molecular diagnosis and classification of inflammatory bowel disease.

Expert Rev Mol Diagn 2018 10 6;18(10):867-886. Epub 2018 Sep 6.

c Center for Inflammatory Bowel Disease, Digestive Disease and Surgery Institute, The Cleveland Clinic Foundation , Cleveland , Ohio , USA.

Introduction: Traditional diagnosis and classification of inflammatory bowel diseases (IBDs) have been based on clinical evaluation, laboratory testing, endoscopy, imaging, and histological examinations. With the advancement of medical technology, an increasing number of molecular surrogates are playing a key role in diagnosis, differential diagnosis, assessment of disease activity, prediction of clinical course, and therapeutic response of IBD. Areas covered: The authors review roles of both existing and emerging surrogates including genetic, serological, histologic, and fecal markers in diagnosis and classification of IBD. Read More

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http://dx.doi.org/10.1080/14737159.2018.1516549DOI Listing
October 2018
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Clinical and molecular diagnosis of pathologic complete response in rectal cancer: an update.

Expert Rev Mol Diagn 2018 10 3;18(10):887-896. Epub 2018 Sep 3.

a University of Texas Southwestern Medical Center , Dallas , TX , USA.

Introduction: The standard of care for locally advanced rectal cancer includes neoadjuvant chemoradiation with subsequent total mesorectal excision. This approach has shown various degrees of response to neoadjuvant chemoradiation (ranging from complete response to further tumor growth), which have substantial prognostic and therapeutic implications. A total regression of the tumor is a predictor of superior oncologic outcomes compared with partial responders and non-responders. Read More

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http://dx.doi.org/10.1080/14737159.2018.1514258DOI Listing
October 2018
10 Reads