9,595 results match your criteria Experimental eye research[Journal]


Age-related spatial differences of human lens UV filters revealed by negative ion mode MALDI imaging mass spectrometry.

Exp Eye Res 2019 Apr 17. Epub 2019 Apr 17.

Department of Physiology, School of Medical Sciences, University of Auckland, Auckland, New Zealand. Electronic address:

Tryptophan-derived UV filters are predominantly found in the lenses of primates and humans. While protective against UV radiation, aging alters the complement and spatial distributions of human lens UV filters, and a role for UV filters has been suggested in age-related cataract formation. To establish how the spatial distributions of UV filters change in normal human lens aging, matrix assisted laser desorption/ionisation-imaging mass spectrometry (MALDI-IMS) was utilised to map the locations and relative abundance of multiple UV filters simultaneously. Read More

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http://dx.doi.org/10.1016/j.exer.2019.04.016DOI Listing

Investigating the potential of carboxymethyl pullulan for protecting the rabbit eye from systematically induced precorneal tear film damage.

Exp Eye Res 2019 Apr 17. Epub 2019 Apr 17.

Pharmaceutics Division, Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, 147 002, Punjab, India. Electronic address:

The present investigation was aimed to develop a rabbit model for protecting the rabbit eye from systematically induced precorneal tear film (PTF) damage, evaluation of carboxymethyl pullulan for its protective action against PTF damage and its curative potential. For the same, pullulan was modified by carboxymethylation and structural modification was confirmed by spectral attributes. Further, the carboxymethyl pullulan (CMP) solutions (0. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183070
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http://dx.doi.org/10.1016/j.exer.2019.04.017DOI Listing
April 2019
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Pregabalin affords retinal neuroprotection in diabetic rats: Suppression of retinal glutamate, microglia cell expression and apoptotic cell death.

Exp Eye Res 2019 Apr 16. Epub 2019 Apr 16.

Department of Pharmacology and Toxicology, Faculty of Pharmacy (girls), Al-Azhar University, Cairo, Egypt.

Pregabalin is the first drug to receive FDA approval for treating diabetic neuropathic pain. This study investigated the neuroprotective effect of pregabalin in an experimental model of diabetic retinopathy and tested some possible mechanisms underlying the putative neuroprotective effect. Male Wistar rats received streptozotocin (45 mg/kg) to induce type 1 diabetes mellitus. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183023
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http://dx.doi.org/10.1016/j.exer.2019.04.014DOI Listing
April 2019
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Optical coherence tomography angiography (OCT-A) in an animal model of laser-induced choroidal neovascularization.

Exp Eye Res 2019 Apr 16. Epub 2019 Apr 16.

Department of Ophthalmology, University of Bonn, Bonn, Germany.

Aim of the study was to compare optical coherence tomography angiography (OCT-A) and conventional fluorescein angiography (FA) for quantitative analysis of the retinal and choroidal vasculature in the animal model of laser-induced choroidal neovascularization (CNV). Therefore, Dark Agouti rats underwent argon laser photocoagulation to induce CNV at D0. In vivo imaging using combined confocal scanner laser ophthalmoscopy (cSLO)-based FA and OCT-A (Heidelberg Engineering GmbH, Heidelberg, Germany) was performed before and immediately after laser treatment as well as at day 2, 7, 14 and 21. Read More

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http://dx.doi.org/10.1016/j.exer.2019.04.002DOI Listing

Analysis of the proteasome activity and the turnover of the serotonin 2B (HTR2B) receptor in human uveal melanoma.

Exp Eye Res 2019 Apr 16. Epub 2019 Apr 16.

CUO-Recherche, Médecine Régénératrice, Centre de Recherche FRQS du CHU de Québec, Université Laval, Québec, Canada; Centre de Recherche en Organogénèse Expérimentale de l'Université Laval/LOEX, Québec, Canada; Département d'Ophtalmologie, Faculté de Médecine, Université Laval, Québec, QC, Canada. Electronic address:

Uveal melanoma (UM), although a very rare disease, remains a particularly aggressive type of cancer as near 50% of the UM presenting patients will also develop liver metastases within 15 years from the initial diagnostic. One of the most reliable predictive markers of UM at risk of evolving toward the formation of liver lesions is an abnormally elevated level of expression of the transcript encoding the 5-Hydroxytryptamine (serotonin) receptor 2B (HTR2B). In our previous study, we demonstrated that transcription of the HTR2B gene was under the regulatory influences of two transcription factors (TFs), NFI and RUNX1. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835193010
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http://dx.doi.org/10.1016/j.exer.2019.04.013DOI Listing
April 2019
2 Reads

Functional analysis of miR-21-3p, miR-30b-5p and miR-150-5p shuttled by extracellular vesicles from diabetic subjects reveals their association with diabetic retinopathy.

Exp Eye Res 2019 Apr 16. Epub 2019 Apr 16.

Dept of Medical Sciences, University of Turin, Italy. Electronic address:

Microvascular dysfunctions due to altered interactions between endothelial cells (ECs) and pericytes are key-events in the pathogenesis of diabetic retinopathy. Extracellular vesicles (EVs) derived from mesenchymal stem cells cultured in diabetic-like conditions enter pericytes, cause their detachment and migration, and stimulate angiogenesis. We recently showed that EVs from diabetic patients with retinopathy have different miRNA profiling patterns from healthy controls, and determine features of retinopathy in in vitro models of retinal microvasculature. Read More

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http://dx.doi.org/10.1016/j.exer.2019.04.015DOI Listing

Comparative transcriptome analysis of human conjunctiva between normal and conjunctivochalasis persons by RNA sequencing.

Exp Eye Res 2019 Apr 15. Epub 2019 Apr 15.

Department of Ophthalmology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, China.

Conjunctivochalasis (CCH) is a common ocular disease, especially in aged people. However, the molecular mechanism of CCH on transcriptional level has been unclear. In this study, we characterized the transcriptional landscape of human conjunctiva and compared the transcriptome between normal persons (n = 10) and CCH patients (n = 11). Read More

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http://dx.doi.org/10.1016/j.exer.2019.04.005DOI Listing

Mutation in the mouse histone gene Hist2h3c1 leads to degeneration of the lens vesicle and severe microphthalmia.

Exp Eye Res 2019 Apr 13. Epub 2019 Apr 13.

Helmholtz Center Munich, German Research Center for Environmental Health, Institute of Developmental Genetics, D-85764, Neuherberg, Germany. Electronic address:

During an ENU (N-ethyl-N-nitrosourea) mutagenesis screen, we observed a dominant small-eye mutant mouse with viable homozygotes. A corresponding mutant line was established and referred to as Aey69 (abnormality of the eye #69). Comprehensive phenotyping of the homozygous Aey69 mutants in the German Mouse Clinic revealed only a subset of statistically significant alterations between wild types and homozygous mutants. Read More

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http://dx.doi.org/10.1016/j.exer.2019.03.024DOI Listing
April 2019
2 Reads

Quantitative fundus autofluorescence in smokers compared to non-smokers.

Exp Eye Res 2019 Apr 13. Epub 2019 Apr 13.

Department of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, MN, United States. Electronic address:

Increased fundus autofluorescence is directly related to increased RPE lipofuscin deposition in the retina and has been observed in eyes with age-related macular degeneration (AMD). Smoking is the most significant modifiable risk factor for the development and progression of AMD, in which one of the main mechanisms is oxidative damage from smoking leading to RPE cell toxicity. The relationship between smoking and autofluorescence is not established and could provide insight into pathogenic mechanism of AMD. Read More

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http://dx.doi.org/10.1016/j.exer.2019.04.004DOI Listing
April 2019
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Molecular markers for corneal epithelial cells in larval vs. adult Xenopus frogs.

Exp Eye Res 2019 Apr 11. Epub 2019 Apr 11.

Department of Cell and Developmental Biology, University of Illinois, 601 S. Goodwin Avenue, Urbana, IL, 61801, USA. Electronic address:

Corneal Epithelial Stem Cells (CESCs) and their proliferative progeny, the Transit Amplifying Cells (TACs), are responsible for maintaining the integrity and transparency of the cornea. These stem cells (SCs) are widely used in corneal transplants and ocular surface reconstruction. Molecular markers are essential to identify, isolate and enrich for these cells, yet no definitive CESC marker has been established. Read More

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http://dx.doi.org/10.1016/j.exer.2019.04.010DOI Listing

3D in vitro model for human corneal endothelial cell maturation.

Exp Eye Res 2019 Apr 10. Epub 2019 Apr 10.

Schepens Eye Research Institute/Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, 20 Staniford Street, Boston, MA, 02114, USA. Electronic address:

Corneal endothelium is a cellular monolayer positioned on the Descemet's membrane at the anterior cornea, and it plays a critical role in maintaining corneal clarity. Our present study examines the feasibility of utilizing our 3-dimensional (3D) corneal stromal construct, which consists of human corneal fibroblasts (HCF) and their self-assembled matrix, to observe the development and maturation of human corneal endothelial cells (HCEndoCs) in a co-culture model. Three-dimensional HCF constructs were created by growing the HCFs on Transwell membranes in Eagles' minimum essential medium (EMEM) + 10% FBS + 0. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183086
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http://dx.doi.org/10.1016/j.exer.2019.04.003DOI Listing
April 2019
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PEDF peptides promote photoreceptor survival in rd10 retina models.

Exp Eye Res 2019 Apr 10;184:24-29. Epub 2019 Apr 10.

Section of Protein Structure and Function, NEI, NIH, Bethesda, MD, USA. Electronic address:

The purpose of the study is to evaluate the protective properties of PEDF peptide fragments on rd10 mouse models of retinal degeneration ex vivo. Human recombinant PEDF and synthetic peptides were used. Rd10 retinal explants as well as wild-type retinal explants treated with zaprinast to mimic the rd10 photoreceptor cell death were employed. Read More

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http://dx.doi.org/10.1016/j.exer.2019.04.008DOI Listing

High-throughput screening identifies compounds that protect RPE cells from physiological stressors present in AMD.

Exp Eye Res 2019 Apr 10. Epub 2019 Apr 10.

Department of Ophthalmology and Visual Science, Yale School of Medicine, 300 George St., Suite 8100, New Haven, CT, 06511, USA. Electronic address:

Dysfunction and eventual loss of retinal pigment epithelial (RPE) cells is a hallmark of atrophic age-related macular degeneration (AMD), and linked to oxidative and nitrosative damage. Herein, we use high-throughput screening (HTS) to identify compounds that protect human RPE cells from oxidative stress-induced cell death and elucidate the possible mechanism of action. HTS was used to identify compounds that protect RPE cells from oxidative damage. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183070
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http://dx.doi.org/10.1016/j.exer.2019.04.009DOI Listing
April 2019
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Effect of somatostatin on human retinal pigment epithelial cells permeability.

Exp Eye Res 2019 Apr 10;184:15-23. Epub 2019 Apr 10.

Experimental Ophthalmo-Biology Group, Department Cell Biology and Histology, Faculty of Medicine and Nursing, University of the Basque Country UPV/EHU, Leioa, Spain.

Purpose: To assess the effect of somatostatin (SST) on the permeability of human retinal pigment epithelial cells.

Methods: We conducted two experiments, exposing cells from human-fetal retinal pigment epithelium (hfRPE) cultures to vascular endothelial growth factor (VEGF), with or without SST pretreatment, in one, and to hypoxic conditions, again with or without SST pretreatment, in the other. The paracellular permeability of hfRPE was assessed by measuring transepithelial electrical resistance (TER) and fluorescein isothiocyanate-sodium (FITC-sodium) flux. Read More

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http://dx.doi.org/10.1016/j.exer.2019.04.007DOI Listing
April 2019
4 Reads

Heterogeneity of cultured melanocyte elongation and proliferation factor in bilateral diffuse uveal melanocytic proliferation.

Exp Eye Res 2019 Apr 9. Epub 2019 Apr 9.

Cole Eye Institute, Cleveland Clinic Foundation, Cleveland, OH, USA. Electronic address:

A patient with bilateral diffuse uveal melanocytic proliferation (BDUMP) associated with endometrial cancer was treated with plasmapheresis, but failed therapy with progressive serous retinal detachment. We collected plasma before and after plasmapheresis therapy. Our goal was to determine if the cultured melanocyte elongation and proliferation (CMEP) factor and hepatocyte growth factor (HGF) was present in the IgG enriched fraction and understand why our patient failed plasmapheresis therapy. Read More

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http://dx.doi.org/10.1016/j.exer.2019.04.006DOI Listing

Spatial intratumor heterogeneity in uveal melanoma: Tumor cell subtypes with a presumed invasive potential exhibit a particular epigenetic staining reaction.

Exp Eye Res 2019 Apr 4;182:175-181. Epub 2019 Apr 4.

Department of Ophthalmology, University of Bonn, Ernst-Abbe-Str. 2, 53127, Bonn, Germany. Electronic address:

Cancer evolves from a combination of genetic and epigenetic abnormalities resulting in aberrant gene expression profiles as well as altered epigenomic patterns. Epigenetic alterations such as DNA methylation and histone modification play an important role in tumorigenesis. While in the pathobiology of uveal melanoma (UM) genetic changes have been well characterized, there is growing evidence suggesting that epigenetic changes are also involved. Read More

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http://dx.doi.org/10.1016/j.exer.2019.04.001DOI Listing
April 2019
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Gene expression profile of extraocular muscles following resection strabismus surgery.

Exp Eye Res 2019 Apr 3;182:182-193. Epub 2019 Apr 3.

Department of Clinical Sciences, Ophthalmology, Umeå University, 907 87, Umea, Sweden; Department of Integrative Medical Biology, Section for Anatomy, Umeå University, 901 87, Umea, Sweden. Electronic address:

This paper aims to identify key biological processes triggered by resection surgery in the extraocular muscles (EOMs) of a rabbit model of strabismus surgery by studying changes in gene expression. Resection surgery was performed in the superior rectus of 16 rabbits and a group of non-operated rabbits served as control. Muscle samples were collected from groups of four animals 1, 2, 4 and 6 weeks after surgery and processed for RNA-sequencing and immunohistochemistry. Read More

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http://dx.doi.org/10.1016/j.exer.2019.03.022DOI Listing
April 2019
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Inducible brown/beige adipocytes in retro-orbital adipose tissues.

Exp Eye Res 2019 Apr 1;184:8-14. Epub 2019 Apr 1.

Faculty of Veterinary Medicine, Kitasato University, School of Veterinary Medicine, Towada, Aomori, 034-8628, Japan. Electronic address:

Beige adipocytes and brown adipocytes can generate heat by using mitochondrial uncoupling protein 1 (Ucp1), a thermogenic protein. Browning/beiging is the emergence of beige adipocytes in white adipose tissues (WAT) for cold acclimatization. Here we show the existence of brown/beige adipocytes in retro-orbital WAT in mice. Read More

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http://dx.doi.org/10.1016/j.exer.2019.03.021DOI Listing

Topical bromfenac transiently delays axotomy-induced retinal ganglion cell loss.

Exp Eye Res 2019 Mar 30;182:156-159. Epub 2019 Mar 30.

Departamento de Oftalmología, Facultad de Medicina, Universidad de Murcia, and Grupo de Oftalmología Experimental, Instituto Murciano de Investigación Biosanitaria Virgen de la Arrixaca (IMIB-Virgen de la Arrixaca), Murcia, Spain. Electronic address:

Optic nerve axotomy in rodents allows detailed studies of the effect of different treatments on the survival of central nervous system neurons, the retinal ganglion cells (RGCs). Here we have analyzed the neuroprotective effect of topical bromfenac treatment, a nonsteroidal anti-inflammatory drug (NSAID) used in clinic to ameliorate post-operative inflammation, on axotomized rat RGCs. The left optic nerve of adult rats was subjected to optic nerve crush (ONC). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183089
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http://dx.doi.org/10.1016/j.exer.2019.03.023DOI Listing
March 2019
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APP processing and metabolism in corneal fibroblasts and epithelium as a potential biomarker for Alzheimer's disease.

Exp Eye Res 2019 Mar 28;182:167-174. Epub 2019 Mar 28.

Department of Ophthalmology, Corneal Dystrophy Research Institute, Yonsei University College of Medicine, Seoul, South Korea; Institute of Vision Research, Yonsei University College of Medicine, Seoul, South Korea. Electronic address:

Alzheimer's disease (AD) primarily affects the brain and is the most common form of dementia worldwide. Despite more than a century of research, there are still no early biomarkers for AD. It has been reported that AD affects the eye, which is more accessible for imaging than the brain; however, links with the cornea have not been evaluated. Read More

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http://dx.doi.org/10.1016/j.exer.2019.03.012DOI Listing
March 2019
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EDITORIAL: Special issue on the role of lipid and protein oxidation in retinal degenerations.

Exp Eye Res 2019 Apr;181:313-315

Department of Ophthalmology and Visual Neurosciences, Medical School, University of Minnesota, Minneapolis, MN, USA. Electronic address:

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http://dx.doi.org/10.1016/j.exer.2019.03.010DOI Listing

Repeated retinal photocoagulation in monkeys for the optimization of a laser-induced choroidal neovascularization model.

Exp Eye Res 2019 Mar 27;184:1-7. Epub 2019 Mar 27.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China. Electronic address:

The laser-induced choroidal neovascularization (CNV) model in nonhuman primates has played a critical role in the development of new therapies for neovascular age-related macular degeneration. The widespread use of this model, however, has been limited by its high costs, mainly due to the lower efficiency of animal use. We optimized the CNV model by administering repeated photocoagulation treatments to the same eye of each animal, and preliminarily evaluated this model using an assessment of the efficacy of an anti-vascular endothelial growth factor (VEGF) agent to address this problem. Read More

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http://dx.doi.org/10.1016/j.exer.2019.03.020DOI Listing

Influence of the time of day on axial length and choroidal thickness changes to hyperopic and myopic defocus in human eyes.

Exp Eye Res 2019 Mar 26;182:125-136. Epub 2019 Mar 26.

Optometry and Vision Science, College of Nursing and Health Sciences, Level 1, Sturt North, Flinders University, Sturt Rd, Bedford Park, SA, 5042, Australia. Electronic address:

Research in animal models have shown that exposing the eye to positive or negative spectacle lenses can lead to predictable changes in eye growth. Recent research indicates that brief periods (1-2 h) of monocular defocus results in small, but significant changes in axial length and choroidal thickness of human subjects. However, the effects of the time of day on these ocular changes with defocus are not known. Read More

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http://dx.doi.org/10.1016/j.exer.2019.03.019DOI Listing

Amelioration of visual deficits and visual system pathology after mild TBI with the cannabinoid type-2 receptor inverse agonist SMM-189.

Exp Eye Res 2019 Mar 26;182:109-124. Epub 2019 Mar 26.

Department of Anatomy and Neurobiology, The University of Tennessee Health Science Center, Memphis, TN 38163, United States; Department of Ophthalmology, The University of Tennessee Health Science Center, Memphis, TN 38163, United States. Electronic address:

Mild TBI is often accompanied by visual system dysfunction and injury, which is at least partly caused by microglial neuroinflammatory processes initiated by the injury. Using our focal cranial blast mouse model of closed-skull mild TBI, we evaluated the ability of the cannabinoid type-2 (CB2) receptor inverse agonist SMM-189, which biases microglia from the harmful M1 state to the beneficial M2 state, to mitigate visual system dysfunction and injury after TBI. Male C57BL/6 or Thy1-EYFP reporter mice received a closed-head blast of either 0-psi (sham) or 50-psi to the left side of the cranium. Read More

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http://dx.doi.org/10.1016/j.exer.2019.03.013DOI Listing

Reduction of circular RNA expression associated with human retinoblastoma.

Exp Eye Res 2019 Mar 24. Epub 2019 Mar 24.

Department of Ophthalmology, Xinhua Hospital, Medical School, Shanghai Jiao Tong University, 1665 Kong Jiang Road, Shanghai, 200092, China. Electronic address:

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http://dx.doi.org/10.1016/j.exer.2019.03.017DOI Listing
March 2019
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Intravenous injection of l-aspartic acid β-hydroxamate attenuates choroidal neovascularization via anti-VEGF and anti-inflammation.

Exp Eye Res 2019 Mar 24;182:93-100. Epub 2019 Mar 24.

School of Optometry and Ophthalmology and the Eye Hospital, Wenzhou Medical University, China; State Key Laboratory of Optometry, Ophthalmology, and Visual Science, Wenzhou, Zhejiang, 325027, China. Electronic address:

Choroidal neovascularization (CNV) is a hallmark of exudative age-related macular degeneration (exAMD) and a major cause of visual loss in AMD. Despite the widespread use of anti-VEGF therapy, serious adverse effects arise from repeated intravitreal injection of anti-VEGF antibodies, which warrant alternative strategy. We report herein that in a CNV murine model created by krypton red laser, intravenous injection of a serine racemase inhibitor, l-Aspartic acid β-hydroxamate (L-ABH), significantly reduced CNV at the dose 6 mg/kg on the first day before and followed by 3 mg/kg on the third day after laser injury. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835193001
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http://dx.doi.org/10.1016/j.exer.2019.03.018DOI Listing
March 2019
38 Reads

Expansion and maintenance of primary corneal epithelial stem/progenitor cells by inhibition of TGFβ receptor I-mediated signaling.

Exp Eye Res 2019 Mar 23;182:44-56. Epub 2019 Mar 23.

Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China. Electronic address:

Transforming growth factor β (TGFβ) signaling is one of the most important signaling pathways regulating cell behavior in ocular tissues. Its functions are mainly linked to tissue fibrosis and inflammatory responses in ophthalmology. In epithelial cells, however, the growth inhibitory activity of TGFβ was reported in both non-ocular and ocular tissues. Read More

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http://dx.doi.org/10.1016/j.exer.2019.03.014DOI Listing

Differential effects of dexamethasone and indomethacin on Tenon's capsule fibroblasts: Implications for glaucoma surgery.

Exp Eye Res 2019 Mar 23;182:65-73. Epub 2019 Mar 23.

Schulich School of Medicine and Dentistry Department of Ophthalmology, London, Ontario, Canada; Schulich School of Medicine and Dentistry Department of Pathology and Laboratory Medicine, London, Ontario, Canada; Ivey Eye Institute, St. Joseph's Healthcare, London, Ontario, Canada.

Dysregulated wound healing and subsequent fibrosis represents the most common cause of failure in glaucoma filtration surgery. Primary means to prevent this outcome are the anti-metabolite surgical adjuvants, however, topical corticosteroids are commonly used postoperatively to permit further control of wound healing and development of the filtration bleb. Unfortunately, they carry important side effects such as raised intraocular pressure, cataract and increased infection risk. Read More

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http://dx.doi.org/10.1016/j.exer.2019.03.015DOI Listing

Matrix metalloproteinases in keratoconus - Too much of a good thing?

Exp Eye Res 2019 Mar 23;182:137-143. Epub 2019 Mar 23.

Section of Ophthalmology and Neuroscience, Leeds Institute of Medical Research, University of Leeds, Wellcome Trust Brenner Building, Beckett Street, Leeds, LS9 7TF, United Kingdom. Electronic address:

Keratoconus (KC) is a progressive, early onset, and often bilateral eye condition, in which the cornea gradually weakens and bulges out, and in advanced cases may eventually become cone-shaped. The available evidence suggests that it is a multifactorial disease with environmental and genetic contributions. Matrix Metalloproteinases (MMPs) are a family of 24 zinc-dependent proteases with the ability to degrade collagen and other extracellular matrix (ECM) proteins, which are important components of the cornea. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183079
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http://dx.doi.org/10.1016/j.exer.2019.03.016DOI Listing
March 2019
2 Reads

Cannabinoid CB2R receptors are upregulated with corneal injury and regulate the course of corneal wound healing.

Exp Eye Res 2019 Mar 21;182:74-84. Epub 2019 Mar 21.

The Gill Center for Biomolecular Science and the Department of Psychological and Brain Sciences, Indiana University, Bloomington, IN, 47405, USA. Electronic address:

CB2R receptors have demonstrated beneficial effects in wound healing in several models. We therefore investigated a potential role of CB2R receptors in corneal wound healing. We examined the functional contribution of CB2R receptors to the course of wound closure in an in vivo murine model. Read More

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http://dx.doi.org/10.1016/j.exer.2019.03.011DOI Listing

Function-first ligandomics for ocular vascular research and drug target discovery.

Exp Eye Res 2019 Mar 21;182:57-64. Epub 2019 Mar 21.

Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami Miller School of Medicine, Miami, FL, USA. Electronic address:

Human eyes may develop different vascular diseases with neovascularization and/or leakage, including wet age-related macular degeneration (AMD), diabetic macular edema (DME), proliferative diabetic retinopathy (PDR), retinopathy of prematurity, corneal neovascularization and intraocular tumors. A breakthrough in therapy is the advent and approval of vascular endothelial growth factor (VEGF) inhibitors. However, anti-VEGF drugs not only have limited efficacy to treat AMD, DME and PDR but also are not approved for other ocular indications. Read More

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http://dx.doi.org/10.1016/j.exer.2019.03.009DOI Listing
March 2019
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Phenotypes of primary retinal macroglia: Implications for purification and culture conditions.

Exp Eye Res 2019 Mar 19;182:85-92. Epub 2019 Mar 19.

Vanderbilt Eye Institute, Vanderbilt University Medical Center, Nashville, TN, 37232, USA; Department of Ophthalmology & Visual Sciences, Vanderbilt University, Nashville, TN, 37232, USA. Electronic address:

Many neurodegenerations, including those of the visual system, have complex etiologies that include roles for both neurons and glia. In the retina there is evidence that retinal astrocytes play an important role in neurodegeneration. There are several approaches for isolating and growing primary retinal astrocytes, however, they often lead to different results. Read More

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http://dx.doi.org/10.1016/j.exer.2019.03.008DOI Listing

Cholesterol modification of SDF-1-specific siRNA enables therapeutic targeting of angiogenesis through Akt pathway inhibition.

Exp Eye Res 2019 Mar 18. Epub 2019 Mar 18.

Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China; Department of Laboratory, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China. Electronic address:

Neovascularization during ocular tissue repair can cause severe visual loss in the optical axis and is therefore an issue of considerable concern to ophthalmologists. Here, we introduced a cholesterol-modified siRNA delivery system targeting stromal cell-derived factor 1 (SDF-1) to treat ocular angiogenesis in vivo. SDF-1 expression was analyzed in rat endothelial progenitor cells (EPCs) and bone marrow mesenchymal stem cells (BMSCs) using quantitative PCR (qPCR). Read More

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http://dx.doi.org/10.1016/j.exer.2019.03.006DOI Listing
March 2019
4 Reads

Involvement of matrix metalloproteinases in capillary degeneration following NMDA-induced neurotoxicity in the neonatal rat retina.

Exp Eye Res 2019 Mar 16;182:101-108. Epub 2019 Mar 16.

Department of Molecular Pharmacology, Kitasato University School of Pharmaceutical Sciences, 5-9-1 Shirokane, Minato-ku, Tokyo, 108-8641, Japan. Electronic address:

Interactions between neuronal cells and vascular cells in the retina are critical for maintaining retinal tissue homeostasis. Impairment of cellular interactions contributes to development and progression of retinal diseases. Previous studies demonstrated that neuronal cell damage leads to capillary degeneration in an N-methyl-D-aspartic acid (NMDA)-induced retinal degeneration model. Read More

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http://dx.doi.org/10.1016/j.exer.2019.03.005DOI Listing

Broad spectrum metabolomics for detection of abnormal metabolic pathways in a mouse model for retinitis pigmentosa.

Exp Eye Res 2019 Mar 15. Epub 2019 Mar 15.

Eastern Regional Comprehensive Metabolomics Resource Core, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; RTI International, 3040 Cornwallis Drive, Research Triangle Park, NC, 27709, USA. Electronic address:

Retinitis pigmentosa (RP) is a degenerative disease of the retina that affects approximately 1 million people worldwide. There are multiple genetic causes of this disease, for which, at present, there are no effective therapeutic strategies. In the present report, we utilized broad spectrum metabolomics to identify perturbations in the metabolism of the rd10 mouse, a genetic model for RP that contains a mutation in Pde6β. Read More

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http://dx.doi.org/10.1016/j.exer.2019.03.007DOI Listing
March 2019
3 Reads

Wnt signaling induces neurite outgrowth in mouse retinal ganglion cells.

Exp Eye Res 2019 Mar 14;182:39-43. Epub 2019 Mar 14.

Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, 33136, USA. Electronic address:

Wingless-type (Wnt) signaling pathways mediate axonal growth and remodeling in the embryonic optic nerve, brain and spinal cord. Recent studies demonstrated that the canonical Wnt/β-catenin signaling pathway also induces axonal regeneration after injury in the optic nerve of adult animals. However, the molecular mechanisms of Wnt-mediated axonal growth are not well understood. Read More

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http://dx.doi.org/10.1016/j.exer.2019.03.004DOI Listing

The vasoreparative potential of endothelial colony-forming cells in the ischemic retina is enhanced by cibinetide, a non-hematopoietic erythropoietin mimetic.

Exp Eye Res 2019 Mar 12;182:144-155. Epub 2019 Mar 12.

Centre for Experimental Medicine, Queen's University Belfast, Belfast, United Kingdom. Electronic address:

Purpose: Retinal ischemia remains a common sight threatening end-point in blinding diseases such as diabetic retinopathy and retinopathy of prematurity. Endothelial colony forming cells (ECFCs) represent a subpopulation of endothelial progenitors with therapeutic utility for promoting reparative angiogenesis in the ischaemic retina. The current study has investigated the potential of enhancing this cell therapy approach by the dampening of the pro-inflammatory milieu typical of ischemic retina. Read More

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http://dx.doi.org/10.1016/j.exer.2019.03.001DOI Listing

Retinal long term neuroprotection by Cerium Oxide nanoparticles after an acute damage induced by high intensity light exposure.

Exp Eye Res 2019 Mar 10;182:30-38. Epub 2019 Mar 10.

Department of Biotechnology and Applied Clinical Sciences, University of L'Aquila, via Vetoio, Coppito 2, 67100, L'Aquila, Italy. Electronic address:

Cerium Oxide nanoparticles are antioxidant agents with autoregenerative radical scavenging activities, effective in preventing degeneration of photoreceptors of an albino rat when intravitreally injected prior to exposure to high intensity light. In this study, we performed a post injury administration of nanoceria and a long term analysis of their neuroprotective properties in order to better simulate the therapeutic treatment as it is carried out on patients with age related macular degeneration, and while photoreceptor degeneration is ongoing. We also injected nanoceria labelled with fluorescein isothiocianate in order to analyze their persistence after a single administration in a damaged retina and to investigate how long they both maintain their neuroprotective properties and where they localize in the retina. Read More

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http://dx.doi.org/10.1016/j.exer.2019.03.003DOI Listing

Interactions of thymic stromal lymphopoietin with TLR2 and TLR4 regulate anti-fungal innate immunity in Aspergillus fumigatus-induced corneal infection.

Exp Eye Res 2019 Mar 8;182:19-29. Epub 2019 Mar 8.

Department of Ophthalmology, Qilu Hospital of Shandong University, Jinan, Shandong, PR China. Electronic address:

Thymic stromal lymphopoietin (TSLP) is an interleukin 7 (IL-7)-like four helix bundle cytokine that plays diverse roles in the regulation of immune responses. In fungal infection, pattern recognition receptors (PRRs), including the cell surface Toll-like receptors (TLRs) and cytoplasmic NOD-like receptors, recognize pathogen-associated molecular patterns to initiate downstream signal cascades to active immune responses. Our previous studies reported that, in vitro human cornea epithelium cells represented a novel target of TSLP and that TSLP/TSLPR/STAT5 signaling played an important role in the response to Aspergillus fumigatus challenge. Read More

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http://dx.doi.org/10.1016/j.exer.2019.02.020DOI Listing
March 2019
2 Reads

The utility of massively parallel sequencing for posterior polymorphous corneal dystrophy type 3 molecular diagnosis.

Exp Eye Res 2019 Mar 7;182:160-166. Epub 2019 Mar 7.

Research Unit for Rare Diseases, Department of Paediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 2, 128 08, Prague 2, Czech Republic; UCL Institute of Ophthalmology, 11-43 Bath Street, EC1V 9EL, London, United Kingdom; Department of Ophthalmology, First Faculty of Medicine, Charles University and General University Hospital in Prague, U Nemocnice 2, 128 08, Prague, Czech Republic. Electronic address:

The aim of this study was to identify the molecular genetic cause of disease in posterior polymorphous corneal dystrophy (PPCD) probands of diverse origin and to assess the utility of massively parallel sequencing in the detection of ZEB1 mutations. We investigated a total of 12 families (five British, four Czech, one Slovak and two Swiss). Ten novel and two recurrent disease-causing mutations in ZEB1, were identified in probands by Sanger (n = 5), exome (n = 4) and genome (n = 3) sequencing. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183078
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http://dx.doi.org/10.1016/j.exer.2019.03.002DOI Listing
March 2019
10 Reads
2.709 Impact Factor

Negative charge at aspartate 151 is important for human lens αA-crystallin stability and chaperone function.

Exp Eye Res 2019 Mar 5;182:10-18. Epub 2019 Mar 5.

Institute for Integrated Radiation and Nuclear Science, Kyoto University, Kumatori-cho, Sennan-gun, Osaka, 590-0494, Japan. Electronic address:

Aggregation of lens protein is a major cause of senile cataract. Lens crystallins contain many kinds of modification that accumulate over lifespan. In particular, isomerization of Asp 151 in αA-crystallin has been found in aged lenses; however, its significance is unknown. Read More

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http://dx.doi.org/10.1016/j.exer.2019.02.023DOI Listing

The absence of SIRT3 and SIRT5 promotes the acetylation of lens proteins and improves the chaperone activity of α-crystallin in mouse lenses.

Exp Eye Res 2019 Mar 5;182:1-9. Epub 2019 Mar 5.

Sue Anschutz-Rodgers Eye Center and Department of Ophthalmology, School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado, Anschutz Medical Campus, Aurora, CO, 80045, USA; Department of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado, Anschutz Medical Campus, Aurora, CO, 80045, USA. Electronic address:

Acetylation of lysine residues occurs in lens proteins. Previous studies have shown an improvement in the chaperone activity of αA-crystallin upon acetylation. Sirtuins are NAD-dependent enzymes that can deacylate proteins. Read More

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http://dx.doi.org/10.1016/j.exer.2019.02.024DOI Listing

Low dosage chloroquine protects retinal ganglion cells against glutamate-induced cell death.

Exp Eye Res 2019 Apr 1;181:285-293. Epub 2019 Mar 1.

Department of Ophthalmology, Shanghai Tenth People's Hospital Affiliated to Tongji University, Shanghai, China. Electronic address:

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183036
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http://dx.doi.org/10.1016/j.exer.2019.02.022DOI Listing
April 2019
3 Reads

Growth hormone-releasing hormone receptor mediates cytokine production in ciliary and iris epithelial cells during LPS-induced ocular inflammation.

Exp Eye Res 2019 Apr 1;181:277-284. Epub 2019 Mar 1.

School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong. Electronic address:

The receptor for growth hormone-releasing hormone (GHRH-R) has been shown to upregulate specifically in the ciliary and iris epithelial cells and infiltrating cells in the aqueous humor in a rat model of acute anterior uveitis. Treatment with GHRHR-R antagonist alleviates significantly these inflammatory responses. Herein we investigated whether the ciliary and iris epithelial cells can respond directly to lipopolysaccharide (LPS) without the influences of circulating leukocytes to produce inflammatory mediators through a GHRH-R mediated mechanism. Read More

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http://dx.doi.org/10.1016/j.exer.2019.02.021DOI Listing
April 2019
2 Reads
2.709 Impact Factor

Staphylococcus aureus alpha-hemolysin impairs corneal epithelial wound healing and promotes intracellular bacterial invasion.

Exp Eye Res 2019 Apr 27;181:263-270. Epub 2019 Feb 27.

Stem Cell Therapy and Corneal Tissue Engineering Laboratory, Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, 1905 W. Taylor St., L-213, Chicago, IL, 60612, United States. Electronic address:

Colonization by Staphylococcus aureus (S. aureus) has been implicated in many infectious and wound healing disorders. This study was performed to characterize the pathogenic role of S. Read More

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http://dx.doi.org/10.1016/j.exer.2019.02.019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447303PMC
April 2019
5 Reads
2.709 Impact Factor

Intravital multiphoton microscopic imaging platform for ocular surface imaging.

Exp Eye Res 2019 Feb 27. Epub 2019 Feb 27.

Department of Ophthalmology, Chang Gung Memorial Hospital, Linkou, Taiwan; College of Medicine, Chang Gung University, Taoyuan, Taiwan. Electronic address:

The purpose of this study is to provide an intravital noninvasive multiphoton microscopic platform for long-term ocular imaging in transgenic fluorescent mice with subcellular resolution. A multiphoton microscopic system with tunable laser output was employed. We designed a mouse holder incorporated with stereotaxic motorized stage for in vivo three-dimensional imaging of ocular surface in 3 transgenic mouse line with fluorescent protein (FP) expression to visualize distinct structures. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183078
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http://dx.doi.org/10.1016/j.exer.2019.02.016DOI Listing
February 2019
3 Reads

In vivo detecting mouse persistent hyperplastic primary vitreous by Spectralis Optical Coherence Tomography.

Exp Eye Res 2019 Apr 25;181:271-276. Epub 2019 Feb 25.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China; Guangdong Province Key Laboratory of Brain Function and Disease, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China. Electronic address:

To identify imaging characteristics of mouse persistent hyperplastic primary vitreous (PHPV) by Spectralis Optical Coherence Tomography (OCT), as well as to assess and compare the sensitivity and precision of OCT with color photography (CP) and Fundus Fluorescein Angiography (FFA) imaging in detecting mouse PHPV. Notch4 C57BL/6J mice (224 eyes) aged from 3 months to 7 months were examined in this study. CP, FFA and OCT imaging were utilized to examine vitreous cavity and retina of mouse eyes. Read More

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http://dx.doi.org/10.1016/j.exer.2019.02.017DOI Listing
April 2019
2.709 Impact Factor

Novelty in limbal stem cell culture and cell senescence.

Exp Eye Res 2019 Apr 23;181:294-301. Epub 2019 Feb 23.

Surgical Oncology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address:

Limbal stem cell deficiency is a pathological state. Recently, limbal stem cell (LSC) transplantation has attracted great interest as a therapeutic method which mainly involves in-vitro expansion of LSCs. It is believed that ex-vivo cultivation conditions could affect the outcome of surgery and the rate of successful transplantation. Read More

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http://dx.doi.org/10.1016/j.exer.2019.02.015DOI Listing
April 2019
3 Reads
2.709 Impact Factor

Ultrastructural analysis of submacular choriocapillaris and its transport systems in AMD and aged control eyes.

Exp Eye Res 2019 Apr 23;181:252-262. Epub 2019 Feb 23.

The Wilmer Ophthalmological Institute, Dept. of Ophthalmology, The Johns Hopkins Hospital, Baltimore, MD, 21287-9915, USA. Electronic address:

The choriocapillaris is the source of nutrients and oxygen for photoreceptors, which consume more oxygen per gram of tissue than any other cell in the body. The purpose of this study was to evaluate and compare the ultrastructure of the choriocapillaris and its transport systems in patients with and without age-related macular degeneration (AMD). Ultrastructural changes were also evaluated in subjects that were homozygous for polymorphisms in high risk CFH alleles (Pure 1) only or homozygous only for high risk ARMS2/HTRA1 (Pure 10) alleles. Read More

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http://dx.doi.org/10.1016/j.exer.2019.02.018DOI Listing
April 2019
2 Reads

Loss-of-function mutations in FREM2 disrupt eye morphogenesis.

Exp Eye Res 2019 Apr 22;181:302-312. Epub 2019 Feb 22.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, 510060, China. Electronic address:

Cryptophthalmos is a rare congenital disorder characterized by ocular dysplasia with eyelid malformation. Complete cryptophthalmos is characterized by the presence of continuous skin from the forehead over the eyes and onto the cheek, along with complete fusion of the eyelids. In the present study, we characterized the clinical manifestations of three patients with isolated bilateral cryptophthalmos. Read More

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http://dx.doi.org/10.1016/j.exer.2019.02.013DOI Listing
April 2019
2 Reads