9,544 results match your criteria Experimental eye research[Journal]


Sirt3 regulates mitophagy level to promote diabetic corneal epithelial wound healing.

Exp Eye Res 2019 Feb 19. Epub 2019 Feb 19.

From the Department of Ophthalmology, Fujian Medical University Union Hospital, Fu Zhou, China.

We aim to investigate how Sirt3 (silent mating type information regulation 2 homolog 3) promoting diabetic corneal epithelial wound healing by regulating mitophagy. The effect of HG(High Glucose, 25 mM D-glucose) on Sirt3 and LC3B(light chain 3 beta)which representing of mitophagy were investigated in TKE2 cells (a murine limbal/corneal epithelium-derived progenitor cell line) and corneal epithelium from C57BL/6J-Ins2Akita (Ins2) mice using RT-PCR and Western blotting. How overexpression of Sirt3 promoting diabetic corneal epithelial wound healing was investigated with cell migration assay、immunofluorescence、 immunofluorescence colocalization and corneal injury model. Read More

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http://dx.doi.org/10.1016/j.exer.2019.02.011DOI Listing
February 2019

BFSP1 C-terminal domains released by post-translational processing events can alter significantly the calcium regulation of AQP0 water permeability.

Exp Eye Res 2019 Feb 18. Epub 2019 Feb 18.

Department of Biosciences, The University of Durham, South Road, Durham, DH1 3LE, UK; Biophysical Sciences Institute, The University of Durham, South Road, Durham, DH1 3LE, UK.

BFSP1 (beaded filament structural protein 1, filensin) is a cytoskeletal protein expressed in the eye lens. It binds AQP0 in vitro and its C-terminal sequences have been suggested to regulate the water channel activity of AQP0. A myristoylated fragment from the C-terminus of BFSP1 was found in AQP0 enriched fractions. Read More

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http://dx.doi.org/10.1016/j.exer.2019.02.001DOI Listing
February 2019

The bidirectional crosstalk between metastatic uveal melanoma cells and hepatic stellate cells engenders an inflammatory microenvironment.

Exp Eye Res 2019 Feb 13. Epub 2019 Feb 13.

Inserm, Université de Rennes 1, UMR 1241, Nutrition, Métabolismes et Cancer (NuMeCan), Rennes, France; Département d'ophtalmologie, Faculté de Médecine, Université Laval, Québec, Canada; Centre Universitaire d'ophtalmologie-Recherche and Axe Médecine Régénératrice, Centre de Recherche du CHU de Québec-Université Laval, Québec, Canada; Centre de Recherche en Organogénèse Expérimentale de l'Université Laval/LOEX, Québec, Canada; Service d'ophtalmologie, CHU de Rennes, Rennes, France. Electronic address:

Uveal melanoma is the most common primary ocular neoplasm in adults. It is peculiar for its hematogenous dissemination and its high propensity to spread to the liver. Current treatments rarely prolong patient survival. Read More

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http://dx.doi.org/10.1016/j.exer.2019.02.012DOI Listing
February 2019
3 Reads

Effects of amantadine on corneal endothelium.

Exp Eye Res 2019 Feb 13. Epub 2019 Feb 13.

Ross Eye Institute, Department of Ophthalmology, Jacobs School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, NY, USA; Research and Ophthalmology Services, Veterans Administration of Western New York Healthcare System, Buffalo, NY, USA. Electronic address:

Purpose: An adverse effect of amantadine, a drug used for Parkinson's disease, is corneal edema. While corneal endothelial cell loss is noted with amantadine toxicity, the reversibility of corneal edema suggests that amantadine affects active mechanisms regulating corneal hydration. Although mainly known as a NMDA receptor antagonist, amantadine is also a K-channel blocker. Read More

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http://dx.doi.org/10.1016/j.exer.2019.02.010DOI Listing
February 2019
1 Read

New unique optical and electric properties of intermediate filaments in Müller cells.

Exp Eye Res 2019 Feb 12. Epub 2019 Feb 12.

University of Puerto Rico, Rio Piedras Campus, PO Box 23343, San Juan, PR 00931-3343, USA. Electronic address:

Presently we report new unique optical and electric properties of Müller cell (MC) intermediate filaments (IFs). We inform that these IFs extracted from porcine retina are excellent conductors of light and electric current. Such IF properties may endow vertebrate eyes with high-contrast vision. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183065
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http://dx.doi.org/10.1016/j.exer.2019.02.008DOI Listing
February 2019
2 Reads

Altered unfolded protein response and proteasome impairment in pseudoexfoliation pathogenesis.

Exp Eye Res 2019 Feb 7;181:197-207. Epub 2019 Feb 7.

School of Biological Sciences, National Institute of Science Education and Research (NISER) Bhubaneswar, HBNI, P.O. Bhimpur-Padanpur, Jatni, Khurda, Odisha, 752050, India. Electronic address:

Pseudoexfoliation (PEX), an ocular disorder involving deposition of proteinaceous fibrils on the surface of anterior eye tissues, is a major contributing factor to worldwide glaucoma. Excessive production and accumulation of fibrillar materials in PEX could be an indication of proteostasis imbalance. This study aims at investigating the differential expression of various genes involved in unfolded protein response and ubiquitin proteasome pathway in pseudoexfoliation (PEX) patients compared to non-PEX controls using lens capsule tissue as the study material. Read More

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http://dx.doi.org/10.1016/j.exer.2019.02.004DOI Listing
February 2019
1 Read

Abdominal obesity linked to a longer cone-mediated dark-adaptation recovery time in healthy eyes.

Exp Eye Res 2019 Feb 6;181:163-170. Epub 2019 Feb 6.

Applied Optics Complutense Group, Faculty of Optics and Optometry, Universidad Complutense de Madrid, Av. Arcos de Jalón 118, Madrid, 28037, Spain.

Obesity has been associated with abnormal lipid metabolism and with tissue hypoxia. Human Bruch's membrane (BrM) lipid deposits have been proposed to create a diffusion barrier to metabolic exchange between the choroid and photoreceptors, delaying the regeneration of photopigments. The speed of retinal dark adaptation (DA) is dependent on the regeneration of these photopigments. Read More

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http://dx.doi.org/10.1016/j.exer.2019.02.007DOI Listing
February 2019

Proteomic evidence that ABCA4 is vital for traumatic proliferative vitreoretinopathy formation and development.

Exp Eye Res 2019 Feb 6. Epub 2019 Feb 6.

Department of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, 1 East Jianshe Road, Zhengzhou, 450052, China. Electronic address:

Proliferative vitreoretinopathy (PVR) is the leading cause of retinal detachment failure. The mechanism of PVR development is complex and still not completely elucidated. There are no proven methods for early prevention or clinical treatment. Read More

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http://dx.doi.org/10.1016/j.exer.2019.02.006DOI Listing
February 2019
1 Read

Permeability of the porcine iris stroma.

Exp Eye Res 2019 Feb 7;181:190-196. Epub 2019 Feb 7.

Ophthalmic Engineering & Innovation Laboratory, Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, Singapore; Singapore Eye Research Institute, Singapore National Eye Centre, Singapore. Electronic address:

Current literature has not considered or provided any data on the permeability of the iris stroma. In this study, we aimed to determine the hydraulic permeability of porcine irides from the isolated stroma. Fifteen enucleated porcine eyes were acquired from the local abattoir. Read More

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http://dx.doi.org/10.1016/j.exer.2019.02.005DOI Listing
February 2019
1 Read
2.709 Impact Factor

Endogenous fluorescence can differentiate the keratoconic cornea.

Exp Eye Res 2019 Feb 5;181:178-184. Epub 2019 Feb 5.

Department of Biophysics and Molecular Physics, A. Chelkowski Institute of Physics, Silesian Center for Education and Interdisciplinary Research, University of Silesia, 41-500 Chorzów, 75 Pułku Piechoty 1A, Poland.

The purpose of the study was to investigate the endogenous fluorescence of the keratoconic cornea in order to analyze changes in the spectra due to the keratoconic stroma abnormalities. Twenty-two corneal buttons obtained from patients with keratoconus (KC, N = 22) at the time of penetrating keratoplasty were used. As a reference, twelve normal corneas (N = 12): ten from the Eye Bank and two from enucleated eyes due to choroidal melanoma were used. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183076
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http://dx.doi.org/10.1016/j.exer.2019.02.002DOI Listing
February 2019
2 Reads

A technical protocol for an experimental ex vivo model using arterially perfused porcine eyes.

Exp Eye Res 2019 Feb 5;181:171-177. Epub 2019 Feb 5.

Department of Pharmaceutical Sciences, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Utrecht, the Netherlands. Electronic address:

Ex vivo ocular perfused models have been described in the past and were applied in different mammalian species as platforms to test drug delivery systems and surgical techniques. However, reproduction of those methods is challenging because extensive and precise description of the protocols used is lacking. In this technical paper we provide a detailed description of all the steps to be followed from the enucleation of porcine eyes to cannulation of the ophthalmic artery and perfusion. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183087
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http://dx.doi.org/10.1016/j.exer.2019.02.003DOI Listing
February 2019
3 Reads

Elevated bone morphogenic protein 4 expression implicated in site-specific adipogenesis in thyroid associated orbitopathy.

Exp Eye Res 2019 Feb 2;181:185-189. Epub 2019 Feb 2.

Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, 4/F, Hong Kong Eye Hospital, 147K Argyle Street, Kowloon, Hong Kong, China; Department of Ophthalmology and Visual Sciences, 1/F, Eye Centre, Prince of Wales Hospital, 30-32 Ngan Shing St, Sha Tin, Hong Kong, China. Electronic address:

Periorbital adipose tissue expansion is a key pathological change in thyroid associated orbitopathy (TAO). Bone morphogenic protein 4 (BMP4) is instrumental in adipogenesis. We compared site-specific BMP4 expression and its effect on adipogenesis using donor-matched adipose tissue-derived stromal cells (ADSC) from TAO patients. Read More

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http://dx.doi.org/10.1016/j.exer.2019.01.020DOI Listing
February 2019

Retinal arteriole reactivity in mice lacking the endothelial nitric oxide synthase (eNOS) gene.

Exp Eye Res 2019 Feb 1;181:150-156. Epub 2019 Feb 1.

Department of Pharmacology, University Medical Center, Johannes Gutenberg University Mainz, Obere Zahlbacher Str. 67, 55131, Mainz, Germany.

Dysfunctional vascular endothelial nitric oxide synthase (eNOS) has been proposed to play a main pathophysiological role in various ocular diseases. The aim of the present study was to test the hypothesis that the chronic lack of eNOS impairs endothelium-dependent vasodilation in retinal arterioles. The relevance of eNOS for mediating vascular responses was studied in retinal vascular preparations from eNOS-deficient mice (eNOS-/-) and wild-type controls in vitro. Read More

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http://dx.doi.org/10.1016/j.exer.2019.01.022DOI Listing
February 2019
1 Read

Effect of brilliant Blue-G on cellular stress response in retinal pigment epithelial cells: In vitro.

Exp Eye Res 2019 Feb 1;181:157-162. Epub 2019 Feb 1.

RS Mehta Jain Department of Biochemistry and Cell Biology, KBIRVO, Vision Research Foundation, Chennai, India. Electronic address:

To assess the cellular stress evoked by exposure of Brilliant Blue-G (BBG), adult retinal pigment epithelial (ARPE-19) cells were treated with various dilutions of BBG in balanced salt solution plus (BSS-PLUS) with and without endoillumination (Alcon Constellation Vision System). The treatments lasted for acute periods of 2 and 5 min. MTT and presto blue assays were performed to assess the changes in cell viability; reactive oxygen species (ROS) production was quantified by DCFDA (dichlorofluorescin diacetate) assay, and the expression of inflammatory stress and endoplasmic reticulum (ER) genes were quantified by qPCR. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183063
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http://dx.doi.org/10.1016/j.exer.2019.01.021DOI Listing
February 2019
1 Read

Animal models of radiation retinopathy - From teletherapy to brachytherapy.

Exp Eye Res 2019 Feb 1. Epub 2019 Feb 1.

Gavin Herbert Eye Institute, 850 Health Sciences Road, Irvine, CA, 92697, USA. Electronic address:

Radiation retinopathy is a serious vision-impairing complication of radiation therapy used to treat ocular tumors. Characterized by retinal vasculopathy and subsequent retinal damage, the first sign of radiation retinopathy is the preferential loss of vascular endothelial cells. Ensuing ischemia leads to retinal degradation and late stage neovascularization. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183071
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http://dx.doi.org/10.1016/j.exer.2019.01.019DOI Listing
February 2019
1 Read

NLRP3 inflammasome in NMDA-induced retinal excitotoxicity.

Exp Eye Res 2019 Jan 29;181:136-144. Epub 2019 Jan 29.

Angiogenesis Laboratory, Retina Service, Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA, USA. Electronic address:

N-methyl-D-aspartate (NMDA)-induced excitotoxicity is an acute form of experimental retinal injury as a result of overactivation of glutamate receptors. NLRP3 (nucleotide-binding domain, leucine-rich-repeat containing family, pyrin domain containing-3) inflammasome, one of the most studied sensors of innate immunity, has been reported to play a critical role in retinal neurodegeneration with controversial implications regarding neuroprotection and cell death. Thus far, it has not been elucidated whether NMDA-mediated excitotoxicity can trigger NLRP3 inflammasome in vivo. Read More

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http://dx.doi.org/10.1016/j.exer.2019.01.018DOI Listing
January 2019

Topical administration of a ROCK inhibitor prevents anterior subcapsular cataract induced by UV-B irradiation.

Exp Eye Res 2019 Jan 26;181:145-149. Epub 2019 Jan 26.

Department of Pharmacotherapeutics, School of Pharmacy, Iwate Medical University, Shiwa-gun, Japan.

The deposition of extracellular matrix (ECM)-which is mainly composed of type I collagen-in anterior subcapsular cataracts (ASCs) during epithelial-to-mesenchymal transition (EMT) of lens epithelial cells (LECs) decreases visual function. Transforming growth factor (TGF)-β is a key factor in the induction of EMT in LECs. Although Rho kinase (ROCK) plays an important role in EMT induced by TGF-β, it is unknown whether ROCK inhibition affects type I collagen expression in TGF-β-stimulated LECs and ASC formation. Read More

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http://dx.doi.org/10.1016/j.exer.2019.01.016DOI Listing
January 2019

Characterization of the novel spontaneously immortalized rat Müller cell line SIRMu-1.

Exp Eye Res 2019 Jan 21;181:127-135. Epub 2019 Jan 21.

School of Biological Sciences, Molecular Life Sciences Building, University of Adelaide, Adelaide, SA, 5005, Australia. Electronic address:

Müller cells (MCs) play a crucial role in the retina, and cultured MC lines are an important tool with which to study MC function. Transformed MC lines have been widely used; however, the transformation process can also lead to unwanted changes compared to the primary cells from which they were derived. To provide an alternative experimental tool, a novel monoclonal spontaneously immortalized rat Müller cell line, SIRMu-1, was derived from primary rat MCs and characterized. Read More

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http://dx.doi.org/10.1016/j.exer.2019.01.013DOI Listing
January 2019
5 Reads

Impact of topical anti-fibrotics on corneal nerve regeneration in vivo.

Exp Eye Res 2019 Jan 17;181:49-60. Epub 2019 Jan 17.

The Flaum Eye Institute, University of Rochester, Rochester, NY, 14642, USA; Center for Visual Science, University of Rochester, Rochester, NY, 14627, USA. Electronic address:

Recent work in vitro has shown that fibroblasts and myofibroblasts have opposing effects on neurite outgrowth by peripheral sensory neurons. Here, we tested a prediction from this work that dampening the fibrotic response in the early phases of corneal wound healing in vivo could enhance reinnervation after a large, deep corneal injury such as that induced by photorefractive keratectomy (PRK). Since topical steroids and Mitomycin C (MMC) are often used clinically for mitigating corneal inflammation and scarring after PRK, they were ideal to test this prediction. Read More

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http://dx.doi.org/10.1016/j.exer.2019.01.017DOI Listing
January 2019
2 Reads

Epithelial cells exert differential traction stress in response to substrate stiffness.

Exp Eye Res 2019 Jan 14;181:25-37. Epub 2019 Jan 14.

Department of Biochemistry, Boston University School of Medicine, Boston, MA, USA; Department of Ophthalmology, Boston University School of Medicine, Boston, MA, USA. Electronic address:

Epithelial wound healing is essential for maintaining the function and clarity of the cornea. Successful repair after injury involves the coordinated movements of cell sheets over the wounded region. While collective migration has been the focus of studies, the effects that environmental changes have on this form of movement are poorly understood. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183078
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http://dx.doi.org/10.1016/j.exer.2019.01.014DOI Listing
January 2019
5 Reads

Comparative localization of cystathionine beta synthases and cystathionine gamma lyase in canine, non-human primate and human retina.

Exp Eye Res 2019 Jan 14;181:72-84. Epub 2019 Jan 14.

Department of Clinical Sciences and Advanced Medicine, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA. Electronic address:

Chronic exposure of the retina to light and high concentrations of polyunsaturated fatty acid in photoreceptor cells make this tissue susceptible to oxidative damage. As retinal degenerative diseases are associated with photoreceptor degeneration, the antioxidant activity of both hydrogen sulfide (HS) and glutathione (GSH) may play an important role in ameliorating disease progression. HS production is driven by cystathionine-γ-lyase (CSE) and cystathionine-β-synthase (CBS), the key enzymes that also drive transsulfuration pathway (TSP) necessary for GSH production. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183083
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http://dx.doi.org/10.1016/j.exer.2019.01.007DOI Listing
January 2019
6 Reads

The association of mitochondrial DNA haplogroups with POAG in African Americans.

Exp Eye Res 2019 Jan 15;181:85-89. Epub 2019 Jan 15.

Scheie Eye Institute, University of Pennsylvania, Philadelphia, PA, USA. Electronic address:

Mitochondrial dysfunction has been implicated in the pathogenesis of primary open-angle glaucoma (POAG). However, the potential significance of mitochondrial DNA (mtDNA) haplogroups to POAG has not been evaluated in the overaffected African American population. To investigate the association of mtDNA haplogroups with POAG and its phenotypic characteristics, genotyping data from 4081 African American subjects (1919 cases and 2162 controls) was analyzed using 1293 positions on mtDNA. Read More

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http://dx.doi.org/10.1016/j.exer.2019.01.015DOI Listing
January 2019
4 Reads

Cone-to-Müller cell ratio in the mammalian retina: A survey of seven mammals with different lifestyle.

Exp Eye Res 2019 Jan 11;181:38-48. Epub 2019 Jan 11.

Paul Flechsig Institute of Brain Research, Medical Faculty, University of Leipzig, Leipzig, Germany.

Mammalian retinal glial (Müller) cells are known to guide light through the inner retina to photoreceptors (Franze et al., 2007; Proc Natl Acad Sci U S A 104:8287-8292). It was shown that Müller cells transmit predominantly red-green and less violet-blue light (Labin et al. Read More

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http://dx.doi.org/10.1016/j.exer.2019.01.012DOI Listing
January 2019
1 Read

Investigation of indoleamine 2,3-dioxygenase 1 expression in uveal melanoma.

Exp Eye Res 2019 Jan 11;181:112-119. Epub 2019 Jan 11.

Research Laboratory of Ophthalmology and Vision Sciences, Torsten-Wiesel Research Institute of World Eye Organization, State Key Laboratory of Biotherapy, West China Hospital, SiChuan University, Chengdu, China; Department of Ophthalmology, West China Hospital, Sichuan University, Cheng Du, Sichuan, China. Electronic address:

The purpose of this study was to investigate indoleamine 2,3-dioxygenase 1 (IDO1) expression and its implications in uveal melanoma (UM). Bioinformatics analysis was performed on microarray data (GSE22138 and GSE27831) from the Gene Expression Omnibus (GEO) database to evaluate IDO1 expression in mRNA level. Ninety-two cases in the database were divided into the IDO1-high group (46 cases) and IDO1-low group (46 cases). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183064
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http://dx.doi.org/10.1016/j.exer.2019.01.005DOI Listing
January 2019
5 Reads

Suppression of neovascularization in corneal stroma in a TRPA1-null mouse.

Exp Eye Res 2019 Jan 8;181:90-97. Epub 2019 Jan 8.

Department of Ophthalmology, Wakayama Medical University, 811-1 Kimiidera, Wakayama, 641-0012, Japan.

Corneal neovascularization and inflammatory fibrosis induced by severe injury or infection leads to tissue opacification and even blindness. Transient receptor potential (TRP) channel subtypes contribute to mediating these maladaptive responses through their interactions with other receptors. TRPV1 is one of the contributing channel isoforms inducing neovascularization in an alkali burn mouse wound healing model. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183008
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http://dx.doi.org/10.1016/j.exer.2019.01.002DOI Listing
January 2019
6 Reads

Expression of growth hormone and growth hormone receptor genes in human eye tissues.

Exp Eye Res 2019 Jan 8;181:61-71. Epub 2019 Jan 8.

Universidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Bioquímica y Medicina Molecular, Monterrey, Nuevo León, Mexico; Vitagénesis, SA, Edificio Vitaxentrum, Blvd. Puerta del Sol 1005, Colinas de San Jerónimo, Monterrey, Nuevo León, 64460, Mexico. Electronic address:

In humans, the polygenic growth hormone (GH) locus is located on chromosome 17 and contributes with three types of proteins: pituitary GH which consists of at least two isoforms one of 22 kDa and the other of 20 kDa, placental GH, which also exhibits isoforms, and chorionic somatomammotropin hormone (CSH). While pituitary GH results from the expression of the GH-1 (GH-N) gene, placental GH is produced by the expression of the GH-2 (GH-V) gene and CSH is contributed by expression of the CSH-1 and CSH-2 genes. The location where GH-1 is expressed is the anterior pituitary and the rest of the genes in the locus are expressed in placenta. Read More

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http://dx.doi.org/10.1016/j.exer.2019.01.011DOI Listing
January 2019
8 Reads
2.709 Impact Factor

Methyl 3,4-dihydroxybenzoate protects retina in a mouse model of acute ocular hypertension through multiple pathways.

Exp Eye Res 2019 Jan 10;181:15-24. Epub 2019 Jan 10.

Guangdong-Hongkong-Macau Institute of CNS Regeneration, Ministry of Education CNS Regeneration Collaborative Joint Laboratory, Jinan University, Guangzhou, 510632, China; Co-Innovation Center of Neuroregeneration, Nantong University, Jiangsu, China. Electronic address:

Methyl 3,4 dihydroxybenzoate (MDHB) is a small molecule that shows neuroprotective effects in vitro and in a photoreceptor-degenerative mouse model. Here we investigated whether MDHB protects retina in a mouse model of acute ocular hypertension (AOH) and explores the underlying mechanisms. AOH was induced in mice by increasing intraocular pressure to approximately 90 mmHg for 60 min, then MDHB or vehicle was intraperitoneally injected daily up to 7 days. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183067
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http://dx.doi.org/10.1016/j.exer.2019.01.010DOI Listing
January 2019
11 Reads

Secretogranin III as a novel target for the therapy of choroidal neovascularization.

Exp Eye Res 2019 Jan 8;181:120-126. Epub 2019 Jan 8.

Bascom Palmer Eye Institute, University of Miami School of Medicine, Miami, FL, USA; Vascular Biology Institute, University of Miami, Miami, FL, USA. Electronic address:

Wet age-related macular degeneration (AMD) with choroidal neovascularization (CNV) is a leading cause of vision loss in the elderly. The advent of anti-vascular endothelial growth factor (VEGF) drugs represents a major breakthrough in wet AMD therapy but with limited efficacy to improve visual acuity. Secretogranin III (Scg3, SgIII) was recently discovered as a novel angiogenic factor with VEGF-independent mechanisms. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183037
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http://dx.doi.org/10.1016/j.exer.2019.01.009DOI Listing
January 2019
10 Reads
2.709 Impact Factor

Effects of time-of-day on inhibition of lens-induced myopia by quinpirole, pirenzepine and atropine in chicks.

Exp Eye Res 2019 Jan 7;181:5-14. Epub 2019 Jan 7.

The New England College of Optometry, 424 Beacon St., Boston, MA, USA.

Injections of the D2 dopamine receptor agonist quinpirole or the acetylcholine muscarinic receptor antagonists pirenzepine and atropine prevent the development of negative-lens-induced myopia in chicks by inhibiting ocular growth. Because ocular growth is diurnally rhythmic, we hypothesized that the efficacy for inhibition may depend on time of day. Chicks wore monocular -10D lenses for 5 days, starting at 12d of age. Read More

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http://dx.doi.org/10.1016/j.exer.2019.01.008DOI Listing
January 2019
1 Read

Identification of a nine-miRNA signature for the prognosis of Uveal Melanoma.

Exp Eye Res 2019 Jan 4;180:242-249. Epub 2019 Jan 4.

Department of Ophthalmology, Inner Mongolia Baogang Hospital, Inner Mongoli, Baotou, 014000, China. Electronic address:

The present study aims to construct a miRNA-based predictive signature of Uveal melanoma (UM) based on the database of the cancer genome atlas (TCGA). We obtained miRNA expression profiles and clinical information of 80 UM patients from TCGA, and randomly divided them into a training and a testing set. After data processing and forward screening, a total of 204 miRNAs with prognostic value were then examined by the Cox proportional hazard regression model in the training set. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183060
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http://dx.doi.org/10.1016/j.exer.2019.01.004DOI Listing
January 2019
8 Reads

Impact of angiogenic activation and inhibition on miRNA profiles of human retinal endothelial cells.

Exp Eye Res 2019 Jan 4;181:98-104. Epub 2019 Jan 4.

Department of Ophthalmology, University Medical Center Greifswald, Germany. Electronic address:

Background: Human retinal microvascular endothelial cells (HRMVECs) are involved in the pathogenesis of retinopathy of prematurity. In this study, the microRNA (miRNA) expression profiles of HRMVECs were investigated under resting conditions, angiogenic stimulation (VEGF treatment) and anti-VEGF treatment.

Materials And Methods: The miRNA profiles of HRMVECs under resting and angiogenic conditions (VEGF treatment), as well as after addition of aflibercept, bevacizumab or ranibizumab were evaluated by analyzing the transcriptome of small non-coding RNAs. Read More

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http://dx.doi.org/10.1016/j.exer.2019.01.006DOI Listing
January 2019
3 Reads

Enhancement of corneal epithelium cell survival, proliferation and migration by red light: Relevance to corneal wound healing.

Exp Eye Res 2019 Jan 3;180:231-241. Epub 2019 Jan 3.

Fundación de Investigación Oftalmológica, Avda. Doctores Fernández-Vega 34, E-33012, Oviedo, Asturias, Spain. Electronic address:

The aim of the present study was to analyse how short wave blue and long wave red light differentially affect corneal epithelial (HCE-2) cells in culture. The corneal epithelium in situ is exposed to more blue light than in the past because of Light Emitting Diodes (LEDs) used for indoor lighting and computer, television and phone screens. Compared with cultures maintained in the dark, low intensity blue light, such as that emitted from computer screens, reduced the proliferation rate of HCE-2 cells and caused cell death at greater intensities in a dose-dependent manner. Read More

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http://dx.doi.org/10.1016/j.exer.2019.01.003DOI Listing
January 2019
2 Reads

Decorin antagonizes corneal fibroblast migration via caveolae-mediated endocytosis of epidermal growth factor receptor.

Exp Eye Res 2019 Jan 3;180:200-207. Epub 2019 Jan 3.

Harry S. Truman Memorial Veteran Hospital, Columbia, MO, 65211, USA; One-Health One-Medicine Ophthalmology and Vision Research Center, College of Veterinary Medicine, University of Missouri, Columbia, MO, 65211, USA.

Decorin (Dcn), a small leucine-rich proteoglycan, is involved in the regulation of corneal wound healing. Epidermal growth factor receptor (EGFR) plays a critical role in corneal fibroblasts proliferation, migration and extracellular matrix (ECM) modulation upon injury or infection. The present study aimed to investigate the mechanistic role of Dcn in EGFR internalization to the regulation of corneal stromal fibroblasts (CSFs) migration, a key step in the corneal wound healing. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183064
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http://dx.doi.org/10.1016/j.exer.2019.01.001DOI Listing
January 2019
6 Reads
2.709 Impact Factor

Lack of cone mediated retinal function increases susceptibility to form-deprivation myopia in mice.

Exp Eye Res 2018 Dec 31;180:226-230. Epub 2018 Dec 31.

Center for Visual and Neurocognitive Rehabilitation, Atlanta VA Healthcare System, 1670 Clairmont Rd, Decatur, GA, 30033, USA; Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 313 Ferst Dr, Atlanta, GA, 30332, USA. Electronic address:

Retinal photoreceptors are important in visual signaling for normal eye growth in animals. We used Gnat2 (Gnat2) mice, a genetic mouse model of cone dysfunction to investigate the influence of cone signaling in ocular refractive development and myopia susceptibility in mice. Refractive development under normal visual conditions was measured for Gnat2 and age-matched Gnat2 mice, every 2 weeks from 4 to 14 weeks of age. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183055
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http://dx.doi.org/10.1016/j.exer.2018.12.021DOI Listing
December 2018
6 Reads

Response to the allegations of figure reuse in Exp Eye Res. 2018; 167: 152-62.

Authors:
Guiqiu Zhao

Exp Eye Res 2018 Dec 31. Epub 2018 Dec 31.

The Affiliated Hospital of Qingdao University, China. Electronic address:

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http://dx.doi.org/10.1016/j.exer.2018.12.015DOI Listing
December 2018
1 Read

Cannabinoid-mediated retinal rescue correlates with improved circadian parameters in retinal dystrophic rats.

Exp Eye Res 2018 Dec 31;180:192-199. Epub 2018 Dec 31.

Physiology, Genetics and Microbiology, University of Alicante, Alicante, Spain; Institute Ramón Margalef, University of Alicante, Alicante, Spain.

Ocular pathologies and blindness have been linked to circadian disorders. In previous studies, our group has demonstrated that retinitis pigmentosa is associated with degenerative changes in the melanopsin system and weaker circadian patterns. We have also shown that cannabinoids preserve retinal structure and function in dystrophic P23H rats. Read More

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http://dx.doi.org/10.1016/j.exer.2018.12.022DOI Listing
December 2018
1 Read

Figure reuse in Exp Eye Res. (2018); 167: 152-62.

Exp Eye Res 2018 Dec 28. Epub 2018 Dec 28.

Department of Immunology, Uppsala University, Sweden. Electronic address:

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http://dx.doi.org/10.1016/j.exer.2018.12.017DOI Listing
December 2018
1 Read

Kojic acid inhibits senescence of human corneal endothelial cells via NF-κB and p21 signaling pathways.

Exp Eye Res 2018 Dec 28;180:174-183. Epub 2018 Dec 28.

Eye Hospital, First Affiliated Hospital, Harbin Medical University, 23 Youzheng Street, Harbin, 150001, China. Electronic address:

Fuchs endothelial dystrophy (FED) and late cornea allograft failure of cornea transplantation are associated with human corneal endothelial cells (HCEC) senescence. Kojic acid has various functions, however, its anti-senescence effect has never been identified. In this study, we investigated the anti-senescence effect of kojic acid on HCEC. Read More

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http://dx.doi.org/10.1016/j.exer.2018.12.020DOI Listing
December 2018
4 Reads

Matching the LenStar optical biometer to A-Scan ultrasonography for use in small animal eyes with application to tree shrews.

Exp Eye Res 2018 Dec 27;180:250-259. Epub 2018 Dec 27.

Department of Ophthalmology and Visual Sciences, The University of Alabama at Birmingham, AL, USA. Electronic address:

We describe an analysis strategy to obtain ultrasonography-matched axial dimensions of small animal eyes using the LenStar biometer. The LenStar optical low-coherence reflectometer is an attractive device for animal research due to its high precision, non-invasiveness, and the ability to measure the axial dimensions of cornea, anterior chamber, lens, vitreous chamber, and axial length. However, this optical biometer was designed for clinical applications in human eyes and its internal analysis provides inaccurate values when used on small eyes due to species-dependent differences in refractive indices and relative axial dimensions. Read More

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http://dx.doi.org/10.1016/j.exer.2018.12.008DOI Listing
December 2018
1 Read

CRB2 mutation causes autosomal recessive retinitis pigmentosa.

Exp Eye Res 2018 Dec 26;180:164-173. Epub 2018 Dec 26.

Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China; Department of Ophthalmology and Vision Science, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, 200023, China; Key Laboratory of Myopia of State Health Ministry (Fudan University) and Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, 200023, China. Electronic address:

Retinitis pigmentosa (RP), the most common form of inherited retinal dystrophies, exhibits significant genetic heterogeneity. The crumbs homolog 2 (CRB2) protein, together with CRB1 and CRB3, belongs to the Crumbs family. Given that CRB1 mutations account for 4% of RP cases, the role of CRB2 mutations in RP etiology has long been hypothesized but never confirmed. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183041
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http://dx.doi.org/10.1016/j.exer.2018.12.018DOI Listing
December 2018
19 Reads

Desmin deficiency is not sufficient to prevent corneal fibrosis.

Exp Eye Res 2018 Dec 24;180:155-163. Epub 2018 Dec 24.

Department of Neuroscience, University of Connecticut Health Center, Farmington, CT, USA. Electronic address:

The type III intermediate filament (IF) proteins vimentin and desmin are sequentially overexpressed in stromal myofibroblasts over the period when fibrosis sets in after corneal injury. Prior findings have revealed vimentin-deficient mice are significantly protected from corneal fibrosis after alkali injury, which has implicated this IF protein as an important regulator of corneal fibrosis. It has remained as yet unproven whether desmin contributes in any significant manner to corneal fibrosis. Read More

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http://dx.doi.org/10.1016/j.exer.2018.12.019DOI Listing
December 2018
2 Reads

Ocular surface pathogenesis associated with precocious eyelid opening and necrotic autologous tissue in mouse with disruption of Prickle 1 gene.

Exp Eye Res 2018 Dec 24;180:208-225. Epub 2018 Dec 24.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, 54 Xianlie South Road, Guangzhou 510060, China. Electronic address:

Ocular surface disease is one major type of eye diseases. Different etiologies trigger distinct pathological responses of the ocular surface. We previously reported that genetically engineered mice with ablation of Prickle 1 manifested precocious eyelid opening with ensuing cornea dysplasia. Read More

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http://dx.doi.org/10.1016/j.exer.2018.12.012DOI Listing
December 2018
1 Read

Association of the SIX6 locus with primary open angle glaucoma in southern Chinese and Japanese.

Exp Eye Res 2018 Dec 23;180:129-136. Epub 2018 Dec 23.

Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong, China; Department of Ophthalmology and Visual Sciences, Prince of Wales Hospital, Hong Kong, China. Electronic address:

The purpose of the study was to evaluate the association profiles of the SIX6 locus with primary open-angle glaucoma (POAG) in southern Chinese and Japanese. In this study, we tested single marker and haplotype-based associations of 11 tagging single nucleotide polymorphisms (SNPs) covering the SIX6 locus with POAG in a Hong Kong Chinese cohort (N = 1402). A novel SNP (i. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183083
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http://dx.doi.org/10.1016/j.exer.2018.12.014DOI Listing
December 2018
9 Reads
2.709 Impact Factor

The effect of part-time wear of 2-zone concentric bifocal spectacle lenses on refractive error development & eye growth in young chicks.

Exp Eye Res 2018 Dec 21;180:184-191. Epub 2018 Dec 21.

School of Optometry and Vision Science, University of California Berkeley, Berkeley, CA, United States.

The purpose of this study is to characterize in young chicks the myopia control effects of part-time wear of two-zone concentric bifocal lenses. Nine-day-old chicks (n = 115) were first made myopic with monocular -10 Diopter (D) single vision (SV) lenses worn for 3 days. Over the 6 days following myopia induction, either 1) two-zone bifocal lenses (-10 D center/-5 D periphery, BFDC) were worn for 12 (full-time), 10, 8, or 6 h, with -10 D SV lenses worn for the remainder of the day, or 2) BFDC or BFNC (-5 D center/-10 D periphery) lenses were worn every other day (EOD). Read More

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http://dx.doi.org/10.1016/j.exer.2018.12.010DOI Listing
December 2018
1 Read

Indocyanine green molecular angiography of choroidal neovascularization.

Exp Eye Res 2018 Dec 21;180:122-128. Epub 2018 Dec 21.

Pharma Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-La Roche, Ltd., Basel, Switzerland. Electronic address:

Retinal diseases such as proliferative diabetic retinopathy and neovascular AMD are characterized by the formation of new blood vessels. Current imaging techniques such as fluorescein and ICG angiography help to identify areas of vascular leakage but are limited in their applicability due to their nonspecific nature. However, as new treatment paradigms emerge in an effort to have patient specific treatments, the development of new imaging techniques that are capable of identifying patient specific biomarkers will become crucial for the success of these approaches. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183078
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http://dx.doi.org/10.1016/j.exer.2018.12.016DOI Listing
December 2018
11 Reads

Visualization of adult fish lens fiber cells.

Exp Eye Res 2018 Dec 21;181:1-4. Epub 2018 Dec 21.

Lund Vision Group, Department of Biology, Lund University, Sölvegatan 35, 22362, Lund, Sweden.

The crystalline lens of a vertebrate eye is a gradient-index lens and grows throughout life by addition of new lens fiber cells in the periphery. In fish, the growing ball-shaped lens maintains sophisticated optical properties throughout life by maintaining the distribution of refractive index relative to the increasing radius of the lens. During this process, the central fibers must increase refractive index by increasing the cytosolic concentration of crystallin proteins. Read More

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http://dx.doi.org/10.1016/j.exer.2018.12.013DOI Listing
December 2018
5 Reads

EyeCi: Optical clearing and imaging of immunolabeled mouse eyes using light-sheet fluorescence microscopy.

Exp Eye Res 2018 Dec 19;180:137-145. Epub 2018 Dec 19.

Dept. of General Zoology, Faculty of Biology, University of Duisburg-Essen, 45141, Essen, Germany; Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), Campus-Vienna-Biocenter 1, 1030, Vienna, Austria.

Immunofluorescent imaging is an indispensable technique to study morphology and molecular aspects in tissues. Classical approaches make it necessary to cut physical sections of tissue samples to overcome the limited penetration depth of light, restricting the available information to two dimensions. Recent advances in tissue-clearing techniques enable imaging of fluorescently labeled organs and entire organisms on a cellular level in three dimensions without the need of sectioning. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183056
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http://dx.doi.org/10.1016/j.exer.2018.12.001DOI Listing
December 2018
17 Reads

Transdifferentiation effects and related mechanisms of nerve growth factor and internal limiting membrane on Müller cells.

Exp Eye Res 2018 Dec 20;180:146-154. Epub 2018 Dec 20.

Department of Ophthalmology, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, 158 Shangtang Road, Hangzhou, Zhejiang, 310014, China. Electronic address:

In this study, we investigated the pro-proliferative, pro-transdifferentiation effects and mechanisms of nerve growth factor (NGF), internal limiting membrane (ILM), and NGF+ILM on Müller cells. The Müller cells cultured with NGF, ILM or both were mediated with tyrosine kinase A (TrkA) (a high affinity receptor for NGF) inhibitor, phosphatidylinositol 3 kinase (PI3K)/protein kinase B (Akt) (an intracellular signaling pathway important in regulating the cell cycle) inhibitor, or LIN28 (a RNA-binding protein and a posttranscriptional regulator of genes involved in developmental timing and self-renewal in embryonic stem cells) siRNA. Immunofluorescence (IF), western blotting (WB) and quantitative real-time polymerase chain reaction (qRT-PCR) were performed to detect the expression of related genes. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183067
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http://dx.doi.org/10.1016/j.exer.2018.12.005DOI Listing
December 2018
17 Reads

A derivative of betulinic acid protects human Retinal Pigment Epithelial (RPE) cells from cobalt chloride-induced acute hypoxic stress.

Exp Eye Res 2018 Dec 19;180:92-101. Epub 2018 Dec 19.

School of Pharmacy, The University of Sydney, NSW, 2006, Australia. Electronic address:

The Retinal Pigment Epithelium (RPE) is a monolayer of cells located above the choroid. It mediates human visual cycle and nourishes photoreceptors. Hypoxia-induced oxidative stress to RPE is a vital cause of retinal degeneration such as the Age-related Macular Degeneration. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183067
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http://dx.doi.org/10.1016/j.exer.2018.12.011DOI Listing
December 2018
14 Reads

YAP and TAZ are distinct effectors of corneal myofibroblast transformation.

Exp Eye Res 2018 Dec 19;180:102-109. Epub 2018 Dec 19.

Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California Davis, Davis, CA, USA; Department of Ophthalmology & Vision Science, School of Medicine, University of California Davis, Davis, CA, USA. Electronic address:

Purpose: Transforming growth factor β1 (TGFβ1) is elevated in wounds after injury and promotes the transdifferentiation of quiescent cells in the stroma (keratocytes, to activated fibroblasts and subsequently myofibroblasts-KFM transformation). Coactivators of transcription, YAP (Yes-associated protein) and TAZ (Transcriptional coactivator with PDZ-binding motif), are mechanotransducers that intersect with the TGFβ pathway via interactions with Smad proteins. Here, we examined the distinct role of YAP and TAZ on TGFβ1 induced myofibroblast transformation of primary human corneal fibroblasts (HCFs). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183065
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http://dx.doi.org/10.1016/j.exer.2018.12.009DOI Listing
December 2018
10 Reads