1,415,084 results match your criteria Experimental biology and medicine Maywood N.J.[Journal]


Elizabethkingia miricola infection in Chinese spiny frog (Quasipaa spinosa).

Transbound Emerg Dis 2018 Dec 16. Epub 2018 Dec 16.

College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan, 611130, China.

Elizabethkingia miricola is a Gram-negative rod which has been incriminated in severe infections in humans. Recently, a serious infectious disease was identified in Chinese spiny frogs (Quasipaa spinosa), in the Sichuan Province of China; the disease was characterized by corneal opacity, the presence of ascites, and neurologic symptoms. A Gram-negative bacillus was isolated from the liver, spleen, and kidney of the diseased frogs. Read More

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December 2018

Alpha and omega in potassium-channel opening.

Authors:
Fredrik Elinder

Acta Physiol (Oxf) 2018 Dec 16:e13240. Epub 2018 Dec 16.

Department of Clinical and Experimental Medicine, Linköping University, Sweden.

In this issue of Acta Physiologica, a study from the laboratory of Peter Larsson shows that the distance between the carboxyl group and the first double bond of polyunsaturated fatty acids (PUFAs) is critical for the affinity of the PUFA molecule to a voltage-gated potassium channel found in the heart . A shorter distance increases the affinity, and the bound PUFA alters the channel's voltage dependence and thereby opens the channel. The efficacy, that is the channel-opening effect of a bound PUFA molecule, partly depends on the distance between the last double bond and the end of the hydrophobic tail. Read More

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December 2018

Hepatitis c late relapse in patients with directly acting antiviral- related sustained virological response at week 12.

Liver Int 2018 Dec 16. Epub 2018 Dec 16.

Laboratory for the identification of prognostic factors of response to the treatment against infectious diseases, University of Campania, Naples, Italy.

Aim: The aim of the present study was to identify, among the patients with failure to DAA regimen, those with a late relapse (after the achievement of a sustained virological response at week 12) and to characterize the clinical, epidemiological and virological features of these patients.

Material And Methods: 129 HCV patients with non-response to an IFN-free regimen were enrolled. Sanger sequencing of NS3, NS5A and NS5B was performed at failure by home-made protocols. Read More

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December 2018

Bringing benign ectomesenchymal odontogenic tumours to the lab: an in vitro study using an organotypic culture model.

J Oral Pathol Med 2018 Dec 16. Epub 2018 Dec 16.

Department of Pathology, Basic Sciences Institute, Universidade Federal de Minas Gerais-UFMG, Belo Horizonte, Brazil.

Background: Benign neoplasms exhibit most of the cellular phenomena considered hallmarks of cancer, except the capacity to metastasize. Thus, the elucidation of the mechanisms associated with the progression of benign neoplasms may complement and clarify the mechanisms involved in carcinogenesis. Benign odontogenic tumours often result in facial deformities and morbidities, and have complex pathogenesis, mainly due to the diversity of interactions between the odontogenic epithelium and the ectomesenchyme. Read More

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December 2018

House dust mite drives pro-inflammatory eicosanoid reprogramming and macrophage effector functions.

Allergy 2018 Dec 16. Epub 2018 Dec 16.

Center of Allergy and Environment (ZAUM), Technical University of Munich and Helmholtz Center Munich, member of the German Center for Lung Research, 80802, Munich, Germany.

Background: Eicosanoid lipid mediators play key roles in type 2 immune responses, e.g. in allergy and asthma. Read More

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December 2018

Autonomous self-fertilization in Linum sulcatum, a native annual with a previously unknown mating system.

J Plant Res 2018 Dec 15. Epub 2018 Dec 15.

Department of Biology, University of Minnesota Duluth, 207A Swenson Science Building, Duluth, MN, 55812, USA.

The mating systems of wild plant populations have profound effects on their genetic structure and evolution, yet remain unknown or incompletely described for many species. One such species, the herbaceous native annual Linum sulcatum Riddell (Linaceae), is thought to be self-compatible, but there has been no experimental evidence to date to support this claim. To assess the breeding system of this species, seeds were collected from wild populations and reared in a controlled environment. Read More

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December 2018

Effect of tree foliage supplementation of tropical grass diet on in vitro digestibility and fermentation, microbial biomass synthesis and enteric methane production in ruminants.

Trop Anim Health Prod 2018 Dec 15. Epub 2018 Dec 15.

Technological Institute of Conkal, Division of Graduate Studies and Research, Avenida Tecnológico s/n, 97345, Conkal, Yucatan, Mexico.

The objective of this study was to evaluate the influence of tree foliage species supplemented in ruminant diets based on Pennisetum purpureum on the in vitro digestibility and fermentation, microbial biomass synthesis and enteric methane production. Seven experimental diets were evaluated, including a control treatment based on P. purpureum (PT) grass, and six additional treatments supplemented with 30. Read More

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December 2018

Italian recommendations for diagnosis and management of congenital myasthenic syndromes.

Neurol Sci 2018 Dec 15. Epub 2018 Dec 15.

Neurology IV - Neuroimmunology and Neuromuscular Diseases Unit, Fondazione IRCCS Istituto Neurologico "Carlo Besta", Via Celoria 11, 20133, Milan, Italy.

Congenital myasthenic syndromes (CMS) are genetic disorders due to mutations in genes encoding proteins involved in the neuromuscular junction structure and function. CMS usually present in young children, but perinatal and adult onset has been reported. Clinical presentation is highly heterogeneous, ranging from mild symptoms to severe manifestations, sometimes with life-threatening respiratory episodes, especially in the first decade of life. Read More

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December 2018

Heterogeneous brain FDG-PET metabolic patterns in patients with C9orf72 mutation.

Neurol Sci 2018 Dec 15. Epub 2018 Dec 15.

Vita-Salute San Raffaele University, Milan, Italy.

Objective: The hexanucleotide repeat expansion in C9orf72 is an associated genetic cause in amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). In the "ALS/FTD" spectrum prevails clinical heterogeneity and an in vivo knowledge of the underling brain dysfunction in patients carrying C9orf72 mutation remain limited and only described at group level. The study aimed to assess the brain metabolic alterations characterizing patients with C9orf72 mutation using FDG-PET in single individuals. Read More

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December 2018

Unhealthy lifestyle and oxidative damage in childhood obesity.

Eat Weight Disord 2018 Dec 15. Epub 2018 Dec 15.

Dietetics and Clinical Nutrition Laboratory, Department of Public Health, Experimental and Forensic Medicine, University of Pavia, Pavia, Italy.

Purpose: Oxidized LDL cholesterol (oxLDL) has been considered as a sensor of oxidative stress (OS) in childhood obesity. We integrated and related our oxLDL existing results previously assessed in overweight/obese children to lifestyle variables to investigate OS-related lifestyle variables.

Methods: 178 Caucasian children/adolescents have been evaluated and according to BMI percentiles have been classified as normal weight (BMI < 75th); overweight (BMI 75-97th) and obese (BMI > 97th). Read More

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December 2018

Bacterial community activity and dynamics in the biofilm of an experimental hybrid wetland system treating greywater.

Environ Sci Pollut Res Int 2018 Dec 15. Epub 2018 Dec 15.

Faculty of Science and Technology, University of Tartu, Vanemuise 46, 51014, Tartu, Estonia.

The objectives of this study were to determine the biofilm microbial activity and bacterial community structure and successions in greywater treatment filters and to relate the treatment efficiency to the bacterial community parameters. This 10-month study was performed in a newly established experimental system for domestic greywater treatment that consisted of three parallel vertical flow filters (VFs) followed by a horizontal flow filter (HF). A rapid increase in the bacterial community abundance occurred during the first 85 days of filter operations, followed by a short-term decrease and the stabilization of the 16S rRNA gene copy numbers at average levels of 1. Read More

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December 2018

Prognostic models for predicting overall survival in metastatic castration-resistant prostate cancer: a systematic review.

World J Urol 2018 Dec 15. Epub 2018 Dec 15.

UroEvidence@Deutsche Gesellschaft für Urologie, Berlin, Germany.

Purpose: Prognostic models are developed to estimate the probability of the occurrence of future outcomes incorporating multiple variables. We aimed to identify and summarize existing multivariable prognostic models developed for predicting overall survival in patients with metastatic castration-resistant prostate cancer (mCRPC).

Methods: The protocol was prospectively registered (CRD42017064448). Read More

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December 2018

Playing Super Mario increases oculomotor inhibition and frontal eye field grey matter in older adults.

Exp Brain Res 2018 Dec 15. Epub 2018 Dec 15.

Centre de Recherche en Neuropsychologie et Cogntion, University of Montreal, Pavillon Marie-Victorin 90, Avenue Vincent d'Indy, Montreal, QC, H2V 2S9, Canada.

Aging is associated with cognitive decline and decreased capacity to inhibit distracting information. Video game training holds promise to increase inhibitory mechanisms in older adults. In the current study, we tested the impact of 3D-platform video game training on performance in an antisaccade task and on related changes in grey matter within the frontal eye fields (FEFs) of older adults. Read More

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December 2018

Sarcoidosis and the Th1 chemokine MIG.

Authors:
F Ragusa

Clin Ter 2018 Nov-Dec;169(6):e308-e313

Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy.

Sarcoidosis is a systemic inflammatory disease, affecting any organ, and that can be discovered by accident in approximately 5% of cases. High levels of the type-1 helper (Th1)-dependent chemokine, monokine induced by interferon (IFN)-γ (MIG)/chemokine (C-X-C motif) ligand (CXCL)9, and its receptor CXCR3 have been reported in bronchoalveolar lavage and biopsy samples of patients with sarcoidosis. These elevated levels are related with the amount of CD4+ lymphocytes and total lymphocytes. Read More

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MIG Th1 chemokine in Vitiligo.

Authors:
G Elia

Clin Ter 2018 Nov-Dec;169(6):e303-e307

Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy.

The importance of the Type-1 helper immune response in the development of Vitiligo (Vit), and of chemokine receptor (CXCR)3 receptor and its chemokine monokine induced by interferon (IFN)-γ(MIG) has been shown by several studies. MIG/ interferon (IFN)-γ-inducible protein 10 (IP10) /CXCR3 axis mediated T-cell recruitment into the skin in Vit is an early event in the progression of the disease. MIG and IP10 circulating levels are increased in progressive Vit. Read More

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MIG in psoriatic arthritis.

Authors:
G Elia

Clin Ter 2018 Nov-Dec;169(6):e297-e302

Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy.

Monokine induced by interferon (IFN)-γ (MIG) / chemokine (C-X-C motif) ligand 9 (CXCL)9 is involved in the pathogenesis of psoriatic arthritis (PsA). It was demonstrated that both blood plasma-derived dendritic cells (pDCs) and pDCs isolated from rheumatoid arthritis (RA) and PsA synovial fluid (SF), expressed CXC receptor (R) 3 and CXCR4, and that the chemotaxis of blood-derived pDCs is stimulated by MIG, (IFN)-γ-inducible protein 10 (IP-10)/CXCL10, IFN-inducible T-cell α chemoattractant (I-TAC) )/CXCL11 and stromal cell-derived factor 1 (SDF-1)/ CXCL12, present in RA and PsA SF. In PsA patients have been found a Th1 immune predominance at early stage of disease, while a reduction of these chemokines has been observed in long lasting PsA, with a significant increase of monocyte chemoattractant protein-1/IP-10 ratio. Read More

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The perception of psychosocial risks through the HSE questionnaire of a population of neurophysiology technicians: a cross-sectional study.

Clin Ter 2018 Nov-Dec;169(6):e281-e286

Department of Public Health and Infectious Diseases, University of Rome "Sapienza", Rome, Italy.

Objectives: The Neurophysiopathology Technicians (NTs) are exposed to psychosocial risks, exposure to psychosocial risks that cause related work stress has been related to numerous physical and mental illnesses. The aim of this study is to evaluate the perception of psychosocial risks in a population of this population.

Materials And Methods: 54 technicians of Neurophysiopathology were enrolled, consisting of 23 males (42,6 %) and 31 females (57,4 %). Read More

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Health status, diseases and vaccinations of the homeless in the city of Palermo, Italy.

Ann Ig 2019 Jan-Feb;31(1):21-34

Department of Science for Health Promotion and Mother-Child Care "G. D'Alessandro", University of Palermo, Palermo, Italy.

Introduction: In Italy, as in the rest of the world, the number of homeless people is increasing considerably. Many of them suffer from chronic conditions, mental health problems and addiction to alcohol, drugs or smoking, and need complex medical care. Their health status is often exacerbated by greater difficulty in accessing primary care. Read More

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Diagnostic Accuracy of Frailty Screening Methods in Advanced Chronic Kidney Disease.

Nephron 2018 Dec 14:1-9. Epub 2018 Dec 14.

Department of Renal Medicine, Lancashire Teaching Hospitals NHS Foundation Trust, Preston, United Kingdom.

Background/aims: Frail patients with chronic kidney disease (CKD) have an increased hospitalisation and mortality rate. However, many popular frailty screening methods have not been validated in patients with CKD. This study evaluates the diagnostic accuracy of several frailty screening methods in patients with CKD G4-5 and those established on haemodialysis (G5D). Read More

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December 2018

In HPV-Positive HNSCC Cells, Functional Restoration of the p53/p21 Pathway by Proteasome Inhibitor Bortezomib Does Not Affect Radio- or Chemosensitivity.

Transl Oncol 2018 Dec 13;12(3):417-425. Epub 2018 Dec 13.

Department of Radiotherapy and Radiation Oncology, Philipps University of Marburg, University Hospital Gießen and Marburg, Baldingerstrasse, 35043 Marburg, Germany; Department of Radiotherapy and Radiation Oncology, University Hospital Jena, Bachstrasse 18, 07743 Jena, Germany. Electronic address:

Human papillomavirus (HPV) associated squamous cell carcinomas of the head and neck region (HPV+ HNSCCs) harbor diverging biological features as compared to classical noxa-induced (HPV-) HNSCC. One striking difference between subtypes is that the tumor suppressor gene TP53 is usually not mutated in HPV+ HNSCCs. However, p53 is inhibited by viral oncoprotein E6, leading to premature proteasomal degradation. Read More

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December 2018

Evodiamine prevents dextran sulfate sodium-induced murine experimental colitis via the regulation of NF-κB and NLRP3 inflammasome.

Biomed Pharmacother 2018 Dec 13;110:786-795. Epub 2018 Dec 13.

College of Veterinary Medicine, Jilin University, Changchun, 130062, People's Republic of China. Electronic address:

Evodiamine (EVO), an extraction from the traditional Chinese medicine Evodia rutaecarpa, has been reported to possess anti-inflammatory, anti-tumor and other pharmacological activities. However, the effectiveness of EVO to relieve dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) has not been evaluated. In this study, the protective effects and mechanisms of EVO on DSS-induced UC mice were investigated. Read More

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December 2018

Inhibition of CTHRC-1 by its specific monoclonal antibody attenuates cervical cancer cell metastasis.

Biomed Pharmacother 2018 Dec 13;110:758-763. Epub 2018 Dec 13.

Department of Obstetrics and Gynecology, Shanghai University of Medicine & Health Sciences Affiliated Sixth People's Hospital South Campus, Shanghai 201499, PR China. Electronic address:

Although combination of surgery and chemo-radiotherapy could cure 80-95% of patients with early cervical cancer, there is still no satisfactory therapeutic strategies for locally advanced and metastatic cervical cancer patients. Our previous study has already investigated that CTHRC-1 is highly expressed not only in the local tissue but also in circulating serum of patients with cervical cancer and played important function on metastasis of cervical cancer cells. In present study, we aimed to see whether circulating specific monoclonal antibody (mAb) to CTHRC-1could inhibit the metastasis of advanced cervical cancer. Read More

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December 2018

Cognitive mechanisms of alexithymia in schizophrenia: Investigating the role of basic neurocognitive functioning and cognitive biases.

Psychiatry Res 2018 Dec 4;271:573-580. Epub 2018 Dec 4.

II Department of Psychiatry, Medical University of Warsaw, Kondratowicza 8, 03-242 Warsaw, Poland.

Alexithymia is an important but poorly understood emotional deficit in schizophrenia. We aimed at investigating the role of basic cognitive functions, cognitive biases, and symptom severity in alexithymia among patients with schizophrenia. Sixty patients (31 females) with schizophrenia were assessed with standardized clinical interviews for symptom severity. Read More

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December 2018

Simultaneous quantification of fifteen compounds in rat plasma by LC-MS/MS and its application to a pharmacokinetic study of Chaihu-Guizhi decoction.

J Chromatogr B Analyt Technol Biomed Life Sci 2018 Dec 8;1105:15-25. Epub 2018 Dec 8.

Shandong University of Traditional Chinese Medicine, Jinan 250355, China. Electronic address:

A simple, sensitive and selective high-performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) method was developed and validated for simultaneous determination and pharmacokinetic study of 15 active compounds (Saikosaponin A, Baicalin, Wogonin, Glycyrrhizic acid, Glycyrrhetinic acid, Albiflorin, Paeoniflorin, Liquiritin, Isoliquiritin, Liquiritigenin, Isoliquiritigenin, Cinnamic acid, Gallic acid, Wogonoside and Oroxylin A) in rat plasma. After a feasible protein precipitation using methanol for sample preparation, chromatographic separation was carried out with a Halo® C column (2.1 × 100 mm, 2. Read More

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December 2018

Innate T cells in the intensive care unit.

Mol Immunol 2018 Dec 13;105:213-223. Epub 2018 Dec 13.

Brigham and Women's Hospital, Pulmonary and Critical Care Medicine, Boston, MA, 02115, United States; Harvard Medical School, Boston, MA, 02115, United States.

Rapid onset of acute inflammation is a hallmark of critical illnesses that bring patients to the intensive care unit (ICU). In critical illness, innate T cells rapidly reach full activation and drive a robust acute inflammatory response. As "cellular adjuvants," innate T cells worsen inflammation and mortality in several common critical illnesses including sepsis, ischemia-reperfusion injury, stroke, and exacerbations of respiratory disease. Read More

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December 2018

N-Alkyl-1,5-dideoxy-1,5-imino-l-fucitols as fucosidase inhibitors: Synthesis, molecular modelling and activity against cancer cell lines.

Bioorg Chem 2018 Dec 4;84:418-433. Epub 2018 Dec 4.

School of Chemistry, National University of Ireland Galway, University Road, Galway H91 TK33, Ireland. Electronic address:

1,5-Dideoxy-1,5-imino-l-fucitol (1-deoxyfuconojirimycin, DFJ) is an iminosugar that inhibits fucosidases. Herein, N-alkyl DFJs have been synthesised and tested against the α-fucosidases of T. maritima (bacterial origin) and B. Read More

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December 2018

Adaptation of Rabensburg virus (RBGV) to vertebrate hosts by experimental evolution.

Virology 2018 Dec 13;528:30-36. Epub 2018 Dec 13.

The Arbovirus Laboratory, Wadsworth Center, New York State Department of Health, Slingerlands, NY, USA; Department of Biomedical Sciences, State University of New York at Albany School of Public Health, Albany, NY, USA.

Rabensburg virus (RBGV; Flaviviridae, Flavivirus) has been classified as both a novel flavivirus and a unique lineage of West Nile virus (WNV). RBGV and WNV share approximately 76% sequence homology, yet RBGV does not replicate to high viral titers within vertebrate cell lines at physiological temperatures and has not been naturally isolated from a vertebrate host. These unique genetic and biological characteristics make RBGV a viable tool to identify the genetic determinants of flavivirus infectivity and fitness in vertebrate hosts. Read More

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December 2018

Long-term disability in neuromyelitis optica spectrum disorder with a history of myelitis is associated with age at onset, delay in diagnosis/preventive treatment, MRI lesion length and presence of symptomatic brain lesions.

Mult Scler Relat Disord 2018 Dec 9;28:64-68. Epub 2018 Dec 9.

Johns Hopkins University, School of Medicine, Department of Neurology, Baltimore, MD, USA.

Background: Neuromyelitis optica spectrum disorder (NMOSD) is an autoimmune disease of the central nervous system (CNS) that preferentially targets the spinal cord and optic nerves. Increasing disability is accrued with each inflammatory attack. Disability has been shown to be an independent predictor of poor quality of life in those with NMOSD. Read More

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December 2018

Association between prenatal opioid exposure, neonatal opioid withdrawal syndrome, and neurodevelopmental and behavioral outcomes at 5-8 months of age.

Early Hum Dev 2018 Dec 13;128:69-76. Epub 2018 Dec 13.

Department of Family and Community Medicine, School of Medicine, University of New Mexico, Albuquerque, NM, United States of America.

Background: While use of prescription opioids and medication assisted therapy (MAT) for opioid use disorder in pregnancy, as well as the incidence of neonatal opioid withdrawal syndrome (NOWS) continue to rise, little is known about outcomes for children with NOWS beyond the newborn period.

Methods: We examined 1) prenatal MAT exposure vs. unexposed healthy controls [HC]; and 2) treatment for NOWS and NOWS severity on infant neurodevelopmental and behavioral outcomes at 5-8 months of age in 78 maternal-infant pairs from the ENRICH prospective cohort study. Read More

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December 2018

A novel experimental approach for liver analysis in rats exposed to Bisphenol A by means of LC-mass spectrometry and infrared spectroscopy.

J Pharm Biomed Anal 2018 Dec 10;165:207-212. Epub 2018 Dec 10.

Department of Experimental Medicine, University of Campania "L. Vanvitelli", Via S. M. di Costantinopoli, 16, 80138, Naples, Italy.

An innovative complementary approach using a liquid chromatographic-mass spectrometer method and infrared spectroscopy is proposed for measuring internal biological exposure to dangerous chemical contaminants and for monitoring biochemical changes in target organs. The proposed methodologies were validated and applied in the case of rats exposed to low-doses of Bisphenol A (BPA). A liquid chromatographic method coupled to a tandem mass spectrometer was used in order to measure BPA concentration in rat livers. Read More

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December 2018

Contraction mode and intensity effects on elbow antagonist muscle co-activation.

J Electromyogr Kinesiol 2018 Dec 8;44:101-107. Epub 2018 Dec 8.

Department of Kinesiology, The University of Toledo, United States.

Introduction: Agonist muscle contraction mode and intensity effects on antagonist co-activation was examined between men and women.

Methods: Fifteen healthy young men (mean ± standard deviation age = 24.9 ± 4. Read More

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December 2018

Increased cerebral blood flow after single dose of antipsychotics in healthy volunteers depends on dopamine D2 receptor density profiles.

Neuroimage 2018 Dec 13. Epub 2018 Dec 13.

Department of Neuroimaging, Institute of Psychiatry, Psychology and Neuroscience, King's College London, SE5 8AF, London, United Kingdom.

As a result of neuro-vascular coupling, the functional effects of antipsychotics in human brain have been investigated in both healthy and clinical populations using haemodynamic markers such as regional Cerebral Blood Flow (rCBF). However, the relationship between observed haemodynamic effects and the pharmacological action of these drugs has not been fully established. Here, we analysed Arterial Spin Labelling (ASL) rCBF data from a placebo-controlled study in healthy volunteers, who received a single dose of three different D2 receptor (DR) antagonists and tested the association of the main effects of the drugs on rCBF against normative population maps of DR protein density and gene-expression data. Read More

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December 2018

ORAL NITAZOXANIDE TREATMENT OF EXPERIMENTAL NEUROCYSTICERCOSIS INDUCES GLUCONEOGENESIS IN TAENIA CRASSICEPS CYSTICERCI.

Acta Trop 2018 Dec 13. Epub 2018 Dec 13.

Tropical Pathology and Public Health Institute, Federal University of Goias, Goiás, Rua 235, s/n, Setor Leste Universitário, CEP: 74605-050, Brazil. Electronic address:

Neurocysticercosis is the most frequent helminthiasis of the central nervous system and is caused by the presence of Taenia solium cysticerci. Nitazoxanide (NTZ) is an antifolate containing the pyrrolopyrimidine-based nucleus that exerts its antiprotozoal activity due to interference with the pyruvate:ferredoxin oxidoreductase (PFOR) enzyme which is essential to anaerobic energy metabolism. The aim of this work was to determine the effect of NTZ on the energetic metabolism of Taenia crassiceps cysticerci intracranially inoculated BALB /c mice. Read More

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December 2018

Effect of dietary supplementation with apple cider vinegar and propionic acid on hemolymph chemistry, intestinal microbiota and histological structure of hepatopancreas in white shrimp, Litopenaeus vannamei.

Fish Shellfish Immunol 2018 Dec 13. Epub 2018 Dec 13.

Iranian Fisheries Science Research Institute. Persian Gulf and Oman Sea Ecology Research Center, Persian Gulf Mollusks Research Station, Bandar-e- Lengeh, Iran.

This experiment was conducted to evaluate the effects of dietary supplementation of Apple cider vinegar (ACV) and propionic acid (PA) on biochemical parameters of hemolymph, intestinal microbiota and histology of hepatopancreas in white shrimp (Litopenaeus vannamei). Five experimental diets were evaluated in this study including diets supplemented with 1.0, 2. Read More

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December 2018

Skim milk powder with high content of Maillard reaction products affect weight gain, organ development and intestinal inflammation in early life in rats.

Food Chem Toxicol 2018 Dec 13. Epub 2018 Dec 13.

Lund University, Faculty of Medicine, Department of Clinical Sciences, Lund, Sweden; Skane University Hospital, Department of Endocrinology, Lund, Sweden. Electronic address:

Background: The intestinal tract is important for development of immune tolerance and disturbances are suggested to trigger autoimmune disorders. The aim of this study was to explore the effect of Maillard products in skim milk powder obtained after long storage, compared to fresh skim milk powder.

Methods: Young rats were weaned onto a diet based on skim milk powder with high concentration of Maillard products (HM-SM, n = 18) or low (C-SM, n = 18) for one week or four weeks. Read More

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December 2018

Ganglioside synthase knock-out reduces prion disease incubation time in mouse models.

Am J Pathol 2018 Dec 13. Epub 2018 Dec 13.

Department of Laboratory Animal Medicine, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan.

Localization of the abnormal and normal isoforms of prion proteins to detergent-resistant membrane microdomains, lipid rafts, is important for the conformational conversion. Lipid rafts are enriched in sialic acid-containing glycosphingolipids, namely gangliosides. Alteration in the ganglioside composition of lipid rafts can affect the localization of lipid raft-associated proteins. Read More

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December 2018

Uridine adenosine tetraphosphate and purinergic signaling in cardiovascular system: an update.

Pharmacol Res 2018 Dec 13. Epub 2018 Dec 13.

Division of Experimental Cardiology, Department of Cardiology, Thoraxcenter, Erasmus University Medical Center, Rotterdam, the Netherlands.

Uridine adenosine tetraphosphate (UpA), biosynthesized by activation of vascular endothelial growth factor receptor (VEGFR) 2, was initially identified as a potent endothelium-derived vasoconstrictor in perfused rat kidney. Subsequently, the effect of UpA on vascular tone regulation was intensively investigated in arteries isolated from different vascular beds in rodents including rat pulmonary arteries, aortas, mesenteric and renal arteries as well as mouse aortas, in which UpA produces vascular contraction. In contrast, UpA produces vascular relaxation in porcine coronary small arteries and rat aortas. Read More

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December 2018

Corneal-PAMPA: A novel, non-cell-based assay for prediction of corneal drug permeability.

Eur J Pharm Sci 2018 Dec 13. Epub 2018 Dec 13.

Chemistry Department, Gedeon Richter Plc, Gyömrői út 19-21, 1107 Budapest, Hungary. Electronic address:

The present study was aimed to develop an in vitro non-cell-based method, for the measurement of corneal permeability. To this avail, the parallel artificial membrane permeability assay (PAMPA) was used for studying the effects of composition of the artificial lipid membrane, the DMSO cosolvent content of the donor phase as well as different buffer solutions in the model. Based on experimental corneal permeability values of 25 active pharmaceutical ingredients (APIs), a final model with good predictive ability (R = 0. Read More

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December 2018

The effect of 1,3-butanediol and carbohydrate supplementation on running performance.

J Sci Med Sport 2018 Dec 6. Epub 2018 Dec 6.

School of Sport, Exercise and Health Sciences, Loughborough University, UK.

Objectives: Ingested ketogenic agents offer the potential to enhance endurance performance via the provision of an alternative exogenous, metabolically efficient, glycogen-sparing fuel (i.e. ketone bodies). Read More

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December 2018

Maini's Many Contributions to Mathematical Enzyme Kinetics: A Review.

Authors:
Meghan A Burke

J Theor Biol 2018 Dec 13. Epub 2018 Dec 13.

Department of Mathematics, Kennesaw State University, 370 Paulding Ave. NW Kennesaw. GA 30144, UK.

Modelling enzyme kinetics has a long history, as one of the earliest areas where mathematics was utilized to understand biological (in this case, biochemical) phenomena. By using differential equations and the law of mass action, many researchers have discovered information about the mechanisms of enzyme workings in the more than 100 years since Leonor Michaelis and Maud Menten published their classic paper in 1913. This work uses the laws obeyed by molecules during a reaction, translates them into mathematical equations, and then compares the results predicted by the equations to data obtained in laboratory conditions. Read More

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December 2018

The challenge of sudden cardiac arrest in patients with structural heart disease: Still a long way to go.

Trends Cardiovasc Med 2018 Nov 27. Epub 2018 Nov 27.

Department of Molecular Medicine, Section of Cardiology, University of Pavia, Pavia, Italy; Cardiac Intensive Care Unit, Arrhythmia and Electrophysiology and Experimental Cardiology, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy. Electronic address:

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November 2018

Relative Left Ventricular Apical Sparing of Longitudinal Strain in Cardiac Amyloidosis: Is it Just Amyloid Infiltration?

JACC Cardiovasc Imaging 2018 Dec 6. Epub 2018 Dec 6.

National Amyloidosis Centre, University College London, Royal Free Hospital, London, United Kingdom.

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December 2018

Ultrasound Bone Fracture Sensing and Data Communication: Experimental Results in a Pig Limb Sample.

Ultrasound Med Biol 2018 Dec 12. Epub 2018 Dec 12.

Frankfurt Initiative for Regenerative Medicine, J. W. Goethe University, Frankfurt am Main, Germany.

Approximately 6.3 million fractures occur each year in the United States alone. Accurately monitoring the progression of fracture healing is essential to be able to advise patients when it is safe to return to normal activity. Read More

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December 2018

Endoscopic Ultrasound-Guided Fine-Needle Injection of Hydrogen Peroxide into the Pancreas: Feasibility and Tolerability Study Using a Survival Porcine Model.

Ultrasound Med Biol 2018 Dec 12. Epub 2018 Dec 12.

Department of Radiology, Gifu University Graduate School of Medicine, Gifu, Japan.

Hydrogen peroxide (HO) has been reported to be an effective radiation sensitizer for various cancers. A combination therapy comprising fine-needle injection (FNI) of HO under endoscopic ultrasound (EUS) guidance and chemoradiation might improve treatment outcomes of pancreatic cancer; however, there have been no reports thus far. The aims of this study were to evaluate the feasibility and safety of EUS-FNI of HO into the pancreas using a porcine survival model. Read More

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December 2018

Mid-term clinical outcomes of out-of-hospital cardiac arrest patients treated with targeted temperature management at 34-36 °C versus 32-34 °C.

Heart Lung 2018 Dec 12. Epub 2018 Dec 12.

Department of Cardiology, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21, Prague, Czech Republic; Third Faculty of Medicine, Charles University, Prague, Czech Republic.

Background: Targeted temperature management (TTM) in comatose survivors of out-of-hospital cardiac arrest has been associated with improved neurological outcomes. However, the optimal temperature target for TTM remains unclear.

Objectives: To compare a TTM protocol targeted at 34-36 °C with a protocol targeted at 32-34 °C with reference to both clinical outcomes and acute complications. Read More

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December 2018

Determination of gas-liquid partition coefficients of several organic solutes in trihexyl (tetradecyl) phosphonium dicyanamide using capillary gas chromatography columns.

J Chromatogr A 2018 Nov 26. Epub 2018 Nov 26.

Laboratorio de Investigación y Desarrollo de Métodos Analíticos (LIDMA) and División Química Analítica, Facultad de Ciencias Exactas, UNLP-CIC-CONICET, 47 and 115 (B1900AJL), Argentina. Electronic address:

We report here gas-liquid partition constants and activity coefficients for thirty-seven volatile organic solutes in ionic liquid trihexyl(tetradecyl)phosphonium dicyanamide measured by gas-liquid chromatography using capillary columns. Four capillary columns with exactly known phase ratios were constructed and employed to measure the solute retention factors at four temperatures between 313.15 and 343. Read More

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November 2018

Immune response in the relapsing-remitting experimental autoimmune encephalomyelitis in mice: The role of the NF-κB signaling pathway.

Cell Immunol 2018 Dec 8. Epub 2018 Dec 8.

Institute of Cell Biophysics, Pushchino, Moscow Region 142290, Russia.

Characteristics of the mouse model of relapsing-remitting experimental autoimmune encephalomyelitis (rEAE) closely resemble manifestations of multiple sclerosis in humans. In the present study, we investigated the mechanisms of inflammatory response, focusing on NF-κB pathway activation. Cytokine response in rEAE mice was multiphasic: the early phase was characterized by the increase in interferon-γ level in plasma. Read More

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December 2018

Risk compensation? The relationship between helmet use and cycling speed under naturalistic conditions.

J Safety Res 2018 Dec 23;67:165-171. Epub 2018 Oct 23.

German Insurers Accident Research (UDV), Berlin, Germany.

Introduction: An argument against mandatory helmet use is based on the idea of risk compensation, which means that cyclists might ride faster when wearing a helmet (Lardelli-Claret et al., 2003). However, questionnaire and experimental studies were unable to find evidence for this assumption (Fyhri et al. Read More

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December 2018

Combined administration of docosahexaenoic acid and thyroid hormone synergistically enhances rat liver levels of resolvins RvD1 and RvD2.

Prostaglandins Leukot Essent Fatty Acids 2019 Jan 29;140:42-46. Epub 2018 Nov 29.

Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile.

Supplementation with omega-3 fatty acids or thyroid hormone (T) exhibit negative effects on inflammatory reactions in experimental animals. The aim of this work was to assess the hypothesis that docosahexaenoic acid (DHA) plus T co-administration enhances liver resolvin (Rv) levels as inflammation resolution mediators. Combined DHA (daily doses of 300 mg/kg for 3 consecutive days)-T (0. Read More

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January 2019

Magnetic chitosan nanocomposite: Fabrication, properties, and optimization for adsorptive removal of crystal violet from aqueous solutions.

Carbohydr Polym 2019 Feb 19;206:844-853. Epub 2018 Nov 19.

Department of Environmental Health Engineering, School of Health and Nutrition, Lorestan University of Medical Sciences, Khorramabad, Iran; Nutritional Health Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.

Recovery of crystal violet (CV) dye was investigated using magnetic chitosan nano-composites (MCNCs) and the effects of process variables (contact time, initial CV concentration, adsorbent dose, and pH) were optimized through response surface methodology. The reliability of the RSM models (first-order model, first model with interaction, the second-order model, and reduced model) was tested by fitting the data. A comparative analysis of the results derived from the models demonstrated that the reduced model was the best. Read More

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February 2019
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