1,947 results match your criteria Experimental and Toxicologic Pathology [Journal]


Esculetin modulates cytotoxicity induced by oxidants in NB4 human leukemia cells.

Exp Toxicol Pathol 2017 Oct 7;69(8):700-712. Epub 2017 Sep 7.

Departamento de Biología de Sistemas, Unidad de Bioquímica y Biología Molecular, Campus Universitario, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain. Electronic address:

Esculetin is a polyphenolic compound with cytoprotective properties. We previously demonstrated the induction of apoptosis by esculetin in NB4 human leukemia cells, as a model, by a mechanism not well understood. To analyse the antioxidant activity of esculetin on apoptosis, we have studied the influence of co-treatments of esculetin at a concentration of 100μM with exogenous ROS donors, namely tert-butyl-hydroperoxide and hydrogen peroxide, on NB4 cells. Read More

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http://dx.doi.org/10.1016/j.etp.2017.08.001DOI Listing
October 2017
40 Reads

Lactobacillus plantarum culture supernatants improve intestinal tissue exposed to deoxynivalenol.

Exp Toxicol Pathol 2017 Oct 1;69(8):666-671. Epub 2017 Aug 1.

Universidade Estadual de Londrina, Laboratory of Animal Pathology, Campus Universitário, Rodovia Celso Garcia Cid, Km 380, Londrina, Paraná 86051-990, Brazil. Electronic address:

In the present study, histological, morphometrical and ultrastructural analysis were performed to investigate intestinal mucosa changes in piglets exposed to deoxynivalenol alone or associated with two strains of Lactobacillus plantarum and the respective culture supernatants. Jejunal explants were incubated for 4h in culture medium with a) only culture medium (DMEM, control group), b) deoxynivalenol (DON, 10μM), c) heat-inactivated Lactobacillus plantarum strain1 - LP1 (1.1×10 CFU/ml) plus DON, d) heat-inactivated Lactobacillus plantarum strain2-LP2 (2. Read More

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http://dx.doi.org/10.1016/j.etp.2017.06.005DOI Listing
October 2017
7 Reads

Sterigmatocystin induced apoptosis in human pulmonary cells in vitro.

Exp Toxicol Pathol 2017 Oct 22;69(8):695-699. Epub 2017 Jul 22.

Department of Pathology, The Second Hospital, Hebei Medical University, Shijiazhuang, China; Laboratory of Pathology, Hebei Medical University, Shijiazhuang, China. Electronic address:

Sterigmatocystin (ST) is generally recognized as a potential carcinogen, mutagen and teratogen. Studies showed that ST could induce adenocarcinoma of lung in mice in vivo and DNA damage, cell cycle arrest in a human immortalized bronchial epithelial cell line (BEAS-2B cells) and a human lung cancer cell line (A549 cells) in vitro. Besides, ST could induce G arrest (cell cycle arrest in G phase) in several other cells. Read More

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http://dx.doi.org/10.1016/j.etp.2017.07.002DOI Listing
October 2017
11 Reads

Ameliorative effect of α-tocopherol on polychlorinated biphenyl (PCBs) induced testicular Sertoli cell dysfunction in F prepuberal rats.

Exp Toxicol Pathol 2017 Oct 22;69(8):681-694. Epub 2017 Jul 22.

Department of Endocrinology, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, 600 113, Tamil Nadu, India; Meenakshi Academy of Higher Education and Research, West KK Nagar, Chennai, 600 078, Tamil Nadu, India. Electronic address:

The study was conducted to investigate the protective role of α-tocopherol against polychlorinated biphenyls (PCBs) induced effect in Sertoli cell function of F prepuberal rats. Dams were grouped into six; each group consists of six animals. Group 1-control treated with corn oil as vehicle, group II- 0. Read More

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http://dx.doi.org/10.1016/j.etp.2017.07.001DOI Listing
October 2017
22 Reads

Anti-arthritic potential of marine macroalgae Turbinaria ornata in Complete Freund's Adjuvant induced rats.

Exp Toxicol Pathol 2017 Oct 3;69(8):672-680. Epub 2017 Jul 3.

Herbal and Indian Medicine Research Laboratory, Department of Biochemistry, Sri Ramachandra University, Porur, Chennai 600 116, India; Natural Products Research Laboratory, Department of Biotechnology, Pondicherry University, Puducherry 605014, India. Electronic address:

T. ornata a macroalgae rich in bioactive molecules possess various biological activities. Herein, the aim of the study is to evaluate the aqueous extract and the sulphated polysaccharide isolated from T. Read More

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http://dx.doi.org/10.1016/j.etp.2017.06.006DOI Listing
October 2017
5 Reads

A study of the correlation between ultrafine particle emissions in motorcycle smoke and mice erythrocyte damages.

Exp Toxicol Pathol 2017 Oct 24;69(8):649-655. Epub 2017 Jun 24.

Physics Department Brawijaya University, Jl. Veteran 65145, Malang, Indonesia. Electronic address:

Sharply increasing of motor vehicles every year contributes to amounts of ultrafine particles (UFPs) in the air. Besides, the existence of UFPs in the blood may cause erythrocyte damages that subject to shape deformation. This study was aimed to investigate the influence of UFPs in the motorcycle smoke exposed to mice in different concentrations to the erythrocyte damages. Read More

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http://dx.doi.org/10.1016/j.etp.2017.06.003DOI Listing
October 2017
9 Reads

The effects of retinoic acid on mmp-2 production, proliferation and ultrastructural morphology in rat uterus.

Exp Toxicol Pathol 2017 Oct 22;69(8):643-648. Epub 2017 Jun 22.

Istanbul University, Istanbul Faculty of Medicine, Histology and Embryology Department, Çapa, Istanbul, Turkey.

Aims: Retinoic acid (RA) has a vital importance in order to ensure continuity and morphology in many tissues. Matrix metalloproteinases (MMPs) have significant roles in proliferation, the formation of cancers, and metastasis. In this study the effects of RA on MMP-2 production in cells of rat uterus were investigated. Read More

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http://dx.doi.org/10.1016/j.etp.2017.06.002DOI Listing
October 2017
13 Reads

Cardiopreventive effect of ethanolic extract of Date Palm Pollen against isoproterenol induced myocardial infarction in rats through the inhibition of the angiotensin-converting enzyme.

Exp Toxicol Pathol 2017 Oct 20;69(8):656-665. Epub 2017 Jun 20.

Laboratory of Plant Biotechnology, Faculty of Sciences of Sfax, B.P.1171, 3000 Sfax, Tunisia.

The present study aimed to examine the putative preventive effect of the ethanolic extract Date Palm Pollen (DPP, Phoenix dactylifera L., family Arecaceae) on isoproterenol-induced myocardial infarction (MI) in rats. Twenty four rats were randomly divided into four groups including control. Read More

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http://dx.doi.org/10.1016/j.etp.2017.06.004DOI Listing
October 2017
42 Reads

In vitro cytotoxic potential of friedelin in human MCF-7 breast cancer cell: Regulate early expression of Cdkn2a and pRb1, neutralize mdm2-p53 amalgamation and functional stabilization of p53.

Exp Toxicol Pathol 2017 Oct 12;69(8):630-636. Epub 2017 Jun 12.

Cancer Molecular Biology Research Lab, Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi Arabia. Electronic address:

We aimed to explore the cytotoxic and apoptotic effect of friedelin on breast cancer MCF-7 cells. Cytotoxic effect of friedelin on MCF-7 cells was analyzed using MTT, cell and nuclear morphology. The apoptosis mechanism of friedelin on MCF-7 cells was analyzed using real-time PCR. Read More

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http://dx.doi.org/10.1016/j.etp.2017.05.011DOI Listing
October 2017
57 Reads

Validating the use of napsin A as a marker for identifying tumorigenic potential of lung bronchiolo-alveolar hyperplasia in rodents.

Exp Toxicol Pathol 2017 Oct 8;69(8):637-642. Epub 2017 Jun 8.

Onco-Pathology, Department of Pathology and Host-Defense, Kagawa University, Kagawa 761-0793, Japan. Electronic address:

There are two types of bronchiolo-alveolar hyperplasia (hyperplasia) in rodent lungs. The first is "inflammatory hyperplasia" that retains its ability to revert to normal epithelia upon removal of the stimulating insult. The second is "latent tumorigenic hyperplasia", which is irreversible and causes independent preneoplastic lesions that can progress to bronchiolo-alveolar adenocarcinoma. Read More

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http://dx.doi.org/10.1016/j.etp.2017.06.001DOI Listing
October 2017
8 Reads

A study on role of triiodothyronine (T3) hormone on the improvement of articular cartilage surface architecture.

Exp Toxicol Pathol 2017 Oct 8;69(8):625-629. Epub 2017 Jun 8.

Department of Joint Surgery, Yantai Yuhuangding Hospital, 264000, China. Electronic address:

The present study was aimed to investigate the effect of triiodothyronine (T3) on the improvement of articular cartilage surface architecture at in vitro level. The T3 hormone was applied to neo-tissues in the range of 50, 100, 150 and 200ng/ml for 5 weeks. At the end of the treatment, biochemical and histological evaluation was carried out in the neo-tissues. Read More

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http://dx.doi.org/10.1016/j.etp.2017.05.010DOI Listing
October 2017
12 Reads

Hepatoprotective effect of chitosan-caffeic acid conjugate against ethanol-treated mice.

Exp Toxicol Pathol 2017 Oct 7;69(8):618-624. Epub 2017 Jun 7.

Department of Marine-Bio Convergence Science, Pukyong National University, Busan 48547, Republic of Korea. Electronic address:

The chitosan-caffeic acid (CCA) conjugate shows a hepatoprotective effect against oxidative stress-induced hepatic damage in cultured hepatocytes. The objective of this study is the verification of the hepatoprotective effect of the CCA in vivo against ethanol-induced liver injury in mice. The administration of ethanol resulted in the increase of the serum-aminotransferase activities (AST and ALT), triglycerides, total cholesterol, and lipid peroxidation. Read More

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http://dx.doi.org/10.1016/j.etp.2017.05.009DOI Listing
October 2017
30 Reads

Combined effects of deoxynivalenol and zearalenone on oxidative injury and apoptosis in porcine splenic lymphocytes in vitro.

Exp Toxicol Pathol 2017 Oct 3;69(8):612-617. Epub 2017 Jun 3.

College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Chengdu, 611130, China;. Electronic address:

Deoxynivalenol (DON) and zearalenone (ZEA) are the two most common mycotoxins in animal feed. In this study, we examined oxidative injury and apoptosis of porcine splenic lymphocytes induced by DON or ZEA and their combination in vitro. Based on IC values, porcine splenic lymphocytes were treated with 0. Read More

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http://dx.doi.org/10.1016/j.etp.2017.05.008DOI Listing
October 2017
35 Reads

Stem cell therapy with skeletal myoblasts accelerates neointima formation in a mouse model of vein graft disease.

Exp Toxicol Pathol 2017 Oct 2;69(8):598-604. Epub 2017 Jun 2.

Department of Cardiac Surgery, Innsbruck Medical University, Innsbruck, Austria.

Although still a matter of controversial discussion, skeletal myoblasts are one of the options for stem cell transplantation improving cardiac function after myocardial infarction, exhibiting several advantages including the availability, the ability of self-renewal and differentiation, and the lack of ethical and immunological problems. The aim of this study was to investigate the impact of stem cell therapy with skeletal myoblasts on experimental venous bypass grafts in a mouse model of vein graft disease. Forty C57BL/6J mice underwent bypass grafting interposing a venous bypass graft of the donor mouse into the carotid artery of the recipient mouse. Read More

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http://dx.doi.org/10.1016/j.etp.2017.05.006DOI Listing
October 2017
21 Reads

Attenuation of thioacetamide-induced hepatocellular injury by short-term repeated injections associated with down-regulation of metabolic enzymes and relationship with MHC class II-presenting cells.

Exp Toxicol Pathol 2017 Oct 27;69(8):589-597. Epub 2017 May 27.

Laboratory of Veterinary Pathology, Osaka Prefecture University, 1-58 Rinku-Ourai-Kita, Izumisano City, Osaka 598-8531, Japan. Electronic address:

The liver is the primary organ participating in the metabolism of xenobiotics and is therefore an important target in the safety assessment of drugs, chemicals and environmental toxins. Drug-induced liver injury (DILI) has recently become widely recognized in human medicine as an adverse event. The progression of DILI often involves "damage-associated molecular patterns" (DAMPs) of gene and protein expression such as high-mobility group boxes (HMGBs), S100 proteins and heat shock proteins (Hsp). Read More

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http://dx.doi.org/10.1016/j.etp.2017.05.005DOI Listing
October 2017
23 Reads

Argininic acid alters markers of cellular oxidative damage in vitro: Protective role of antioxidants.

Exp Toxicol Pathol 2017 Oct 26;69(8):605-611. Epub 2017 May 26.

Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, Porto Alegre, RS, Brazil.

We, herein, investigated the in vitro effects of argininic acid on thiobarbituric acid-reactive substances (TBA-RS), total sulfhydryl content and on the activities of antioxidant enzymes such as catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the blood, kidney and liver of 60-day-old rats. We also verified the influence of the antioxidants (each at 1.0mM) trolox and ascorbic acid, as well as of N-nitro-l-arginine methyl ester (L-NAME) at 1. Read More

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http://dx.doi.org/10.1016/j.etp.2017.05.007DOI Listing
October 2017
20 Reads

Protective effects of melatonin on long-term administration of fluoxetine in rats.

Exp Toxicol Pathol 2017 Oct 25;69(8):564-574. Epub 2017 May 25.

Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

The degree and consequence of tissue injury are highly regarded during long-term exposure to selective antidepressant fluoxetine. Melatonin has been shown to palliate different lesions by scavenging free radicals, but its role in the reduction of the fluoxetine-induced injuries has been little known. Thirty-six mature male Wistar rats were randomly assigned into control and experimental groups. Read More

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http://dx.doi.org/10.1016/j.etp.2017.05.002DOI Listing
October 2017
29 Reads

Clinical and pathological analysis of 19 cases of medullary thyroid carcinoma without an increase in calcitonin.

Exp Toxicol Pathol 2017 Oct 25;69(8):575-579. Epub 2017 May 25.

Zhejiang Cancer Hospital, Hangzhou 310000, Zhejiang, China. Electronic address:

Background: Medullary thyroid carcinoma (MTC), defined as a malignant tumour with C-cell differentiation, is of neuroendocrine origin and is characterized by the synthesis and secretion of calcitonin (CT). MTC without CT secretion has been reported on rare occasions. The purpose of this study was to evaluate the histological, immunohistochemical, and molecular pathologic features as well as the clinical significance of non-secretory MTC (NCR-MTC). Read More

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http://dx.doi.org/10.1016/j.etp.2017.05.003DOI Listing
October 2017
9 Reads

Effects of PCB-126 on aryl hydrocarbon receptor, ubiquitin and p53 expression levels in Sparus aurata.

Exp Toxicol Pathol 2017 May 25. Epub 2017 May 25.

Department of Clinical and Experimental Medicine, University of Messina, Torre Biologica, 5th Floor, AOU Policlinico "G. Martino", Via C. Valeria Gazzi, 98125, Messina, Italy.

The aim of the present study is to determine if Ahr ligands as PCB-126, a dioxin-like, might contribute to inhibition of the tumor suppressor p53 by promoting its degradation through proteasome-ubiquitin system (UPS). The findings show, in the presence of PCB-126, a significant increase of p53 immunoreactivity in fish compared to the control. Subsequently, there is a decrease of p53 immunoreactivity at 24h which is maintained even at 72h. Read More

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http://dx.doi.org/10.1016/j.etp.2017.04.005DOI Listing
May 2017
5 Reads

Protective effect of Hesperidin and Tiger nut against Acrylamide toxicity in female rats.

Exp Toxicol Pathol 2017 Oct 24;69(8):580-588. Epub 2017 May 24.

Chemistry Department, Bio-Chemistry Division, Faculty of Science, El Fayoum University, El- Fayoum, Egypt.

Phytochemicals that have antioxidant effect play important role in protection against several diseases in humans. This study was carried out to evaluate the efficacy of hesperidin and tiger nut against the early changes that may be related to the toxicity of acrylamide in female rats. 72 Sprague Dawley female rats were divided into six groups (12 rat/group): control group (I); hesperidin (HES) treated group (II); tiger nut (TN) treated group (III); Acrylamide (ACR) treated group (IV); HES-ACR treated group (V); and TN-ACR treated group (VI). Read More

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http://dx.doi.org/10.1016/j.etp.2017.05.004DOI Listing
October 2017
19 Reads

Suppression of epithelial restitution using an inhibitor against Rho-associated coiled-coil containing protein kinase aggravates colitis through reduced epithelial expression of A-kinase anchor protein 13.

Exp Toxicol Pathol 2017 Oct 20;69(8):557-563. Epub 2017 May 20.

Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.

In the gastrointestinal tract, the immediate healing response to mucosal damage is critical to sustain mucosal homeostasis. The migration of surrounding epithelial cells to cover the denuded area without proliferation is termed restitution, followed by early reparation of the damage. In this study, we determined the role of A-kinase anchor protein 13 (AKAP13) in mice with dextran sulphate sodium (DSS)-induced colitis upon mucosal injury and restitution, and investigated whether inhibition of Rho-associated coiled-coil containing protein kinase (ROCK), downstream effector of AKAP13, affects these mucosal responses. Read More

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http://dx.doi.org/10.1016/j.etp.2017.05.001DOI Listing
October 2017
11 Reads

Adverse effects in kidney function, antioxidant systems and histopathology in rats receiving monosodium glutamate diet.

Exp Toxicol Pathol 2017 Sep 16;69(7):547-556. Epub 2017 May 16.

Laboratorio de Investigaciones Nutricionales, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Ciudad Universitaria, CC 242, 3000 Santa Fe, Argentina. Electronic address:

We investigated the effects of adding of monosodium glutamate (MSG) to a standard diet on oxidative stress in kidney, nitric oxide excretion, renal ions handling and blood pressure. We examined the association of these changes with the effects on renal histology. The study was performed on male Wistar rats (5 weeks old) divided into 3 groups: 1) MSG group were fed a diet supplemented with 3g of MSG/kg b. Read More

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http://dx.doi.org/10.1016/j.etp.2017.03.003DOI Listing
September 2017
41 Reads

Chicken egg fetal liver DNA and histopathologic effects of structurally diverse carcinogens and non-carcinogens.

Exp Toxicol Pathol 2017 Sep 11;69(7):533-546. Epub 2017 May 11.

New York Medical College, Department of Pathology, Valhalla, NY, 10595, USA. Electronic address:

Chicken egg fetal livers were evaluated for histopathological changes produced by four genotoxic hepatocarcinogens: 2-acetylaminofluorene (AAF), aflatoxin B (AFB), benzo[a]pyrene (BaP), diethylnitrosamine (DEN); four structurally related non- or weakly- carcinogenic comparators: fluorene (FLU), aflatoxin B (AFB), benzo[e]pyrene (BeP), N-nitrosodiethanolamine (NDELA); two epigenetic hepatocarcinogens: clofibric acid (CFA), phenobarbital (PB); and the non-carcinogen, D-mannitol (MAN). CFA, PB and MAN were also assessed for formation of DNA adducts using the P nucleotide postlabeling (NPL) assay and for DNA breaks using the comet assay. CFA was also assessed in enhanced comet assay for oxidative DNA damage induction. Read More

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http://dx.doi.org/10.1016/j.etp.2017.04.011DOI Listing
September 2017
22 Reads

Laminaria Japonica Polysaccharides effectively inhibited the growth of nasopharyngeal carcinoma cells in vivo and in vitro study.

Exp Toxicol Pathol 2017 Sep 10;69(7):527-532. Epub 2017 May 10.

Department of Otolaryngology-Head and Neck Surgery, Ezhou Central Hospital, Ezhou, Hubei 436000, PR China. Electronic address:

Aims: Laminaria Japonica Polysaccharides (LJP) is a kind of plant polysaccharide isolated from Laminaria Japonica Aresch. LJP has a variety of biological activity, including anti-tumor, improving immune function, anti-radiation and others. This study observed the biological activity of LJP in vitro and in vivo on human nasopharyngeal carcinoma(NPC), and the possible anticancer mechanism was explored. Read More

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http://dx.doi.org/10.1016/j.etp.2017.03.005DOI Listing
September 2017
45 Reads

Ameliorative effects of Achillea millefolium inflorescences alcoholic extract against nicotine-induced reproductive failure in rat.

Exp Toxicol Pathol 2017 Sep 8;69(7):504-516. Epub 2017 May 8.

Food and Beverages Safety Research Center. Urmia University of Medical Sciences, Urmia, Iran; Department of Pharmacology & Toxicology. Faculty of Pharmacy. Urmia University of Medical Sciences, Urmia, Iran.

Background: Nicotine (Nic) is a major risk factor in the development of functional disorders of male reproductive system. Achillea millefolium; is highly regarded for medicinal activities, due to its antioxidant and anti-inflammatory properties. This study was carried out to evaluate whether Achillea millefolium (Achm) inflorescences alcoholic extract could serve as a protective agent in male reproductive male failures during Nic exposure in a rat model. Read More

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http://dx.doi.org/10.1016/j.etp.2017.04.012DOI Listing
September 2017
12 Reads

Late effect of developmental exposure to glycidol on hippocampal neurogenesis in mice: Loss of parvalbumin-expressing interneurons.

Exp Toxicol Pathol 2017 Sep 8;69(7):517-526. Epub 2017 May 8.

Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan; Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-hi, Tokyo 183-8509, Japan. Electronic address:

Developmental exposure to glycidol of rats causes axonal injury targeting axon terminals in dams and transient disruption of late-stage differentiation of hippocampal neurogenesis, accompanying sustained increase in the number of reelin-producing or calretinin-expressing interneurons in offspring. The molecular mechanism of disruptive neurogenesis probably targets the newly generating nerve terminals. We previously found differences between mice and rats in the effects on hippocampal neurogenesis after developmental exposure to the same neurotoxic substances. Read More

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http://dx.doi.org/10.1016/j.etp.2017.04.008DOI Listing
September 2017
11 Reads

Liver cirrhosis reversion is improved in hamsters with a neurointermediate pituitary lobectomy.

Exp Toxicol Pathol 2017 Sep 7;69(7):496-503. Epub 2017 May 7.

Departamento de Química, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Mexico. Electronic address:

Regulating mechanisms of fibrosis is an important goal in the treatment of fibrosis and liver cirrhosis. The role of arginine vasopressin (AVP) in promoting fibrosis in several organs has been well documented. However, the result of an AVP deficiency during liver fibrosis has not been reported. Read More

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http://dx.doi.org/10.1016/j.etp.2017.04.006DOI Listing
September 2017
27 Reads

Buffalo casein derived peptide can alleviates HO induced cellular damage and necrosis in fibroblast cells.

Exp Toxicol Pathol 2017 Sep 5;69(7):485-495. Epub 2017 May 5.

Animal Biochemistry Division, ICAR-National Dairy Research Institute, Karnal, Haryana, India. Electronic address:

Oxidative stress is one of a critical pathogenic factor in the progression of aging and chronic diseases such as cancer, myocardial inflammation and diabetes. In the present scenario, peptides with short half life and more biological specificities are gaining much attention as prodrugs. Thus, the present investigation carried out to screen potential antioxidative peptide, VLPVPQK to cope with the cellular oxidative damage. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09402993163033
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http://dx.doi.org/10.1016/j.etp.2017.04.009DOI Listing
September 2017
57 Reads

The potentiality of two-dimensional preantral follicle culture as an in vitro model in predicting premature ovarian failure.

Exp Toxicol Pathol 2017 Sep 5;69(7):477-484. Epub 2017 May 5.

Department of Pharmacology and Toxicology, National Population and Family Planning Key Laboratory of Contraceptives Drugs and Devices, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China; Reproductive and Developmental Research Institute of Fudan University, Shanghai 200032, China. Electronic address:

The purpose of this study is to identify the potential of a two-dimensional preantral follicle culture as an in vitro model of predicting premature ovarian failure. The two-dimensional preantral follicles culture method was established by cultivating preantral follicles collected from ICR F1 hybrids (aged 12-14days) for 12days. The preantral follicles were incubated with 0. Read More

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http://dx.doi.org/10.1016/j.etp.2017.04.010DOI Listing
September 2017
50 Reads

Antioxidant, cytotoxic and antineoplastic effects of Carissa carandas Linn. leaves.

Exp Toxicol Pathol 2017 Sep 3;69(7):469-476. Epub 2017 May 3.

Department of Biochemistry and Molecular Biology, Rajshahi University, Rajshahi 6205, Bangladesh. Electronic address:

For scientific clarification of some traditional uses, this study was designed to explore the antioxidant, cytotoxic and antineoplastic properties of leaf extract of Carissa carandas Linn., a traditional medicinal plant of Bangladesh. The methanol extract of Carissa carandas leaves (MELC) was applied on DPPH and ABTS experiments to determine its antioxidant activity. Read More

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http://dx.doi.org/10.1016/j.etp.2017.03.008DOI Listing
September 2017
16 Reads

Reproductive toxicity of Roundup herbicide exposure in male albino rat.

Exp Toxicol Pathol 2017 Sep 2;69(7):461-468. Epub 2017 May 2.

Department of Pure and Applied Zoology, College of Bioscience, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria.

The incidence of infertility in human is on the increase and the use of Roundup herbicide and presence of its residues in foodstuff is a major concern. This study therefore aim to assess the effect of Roundup on the reproductive capacity of 32 adult male albino rats randomized into 4 groups of 8 rats per group orally exposed to Roundup at 3.6mg/kg body weight(bw), 50. Read More

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http://dx.doi.org/10.1016/j.etp.2017.04.007DOI Listing
September 2017
41 Reads

Expression of A-kinase anchor protein 13 and Rho-associated coiled-coil containing protein kinase in restituted and regenerated mucosal epithelial cells following mucosal injury and colorectal cancer cells in mouse models.

Exp Toxicol Pathol 2017 Sep 20;69(7):443-450. Epub 2017 Apr 20.

Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo, 183-8509, Japan.

We demonstrate the expression patterns of A-kinase anchor protein 13 (AKAP13), a scaffold protein that acts upstream of Rho signaling, and Rho-associated coiled-coil containing protein kinase (ROCK) 1/2 in mouse colorectal cancer and during the healing stage of mouse colitis. BALB/c mice received an intraperitoneal injection of azoxymethane at 10mg/kg, followed by two 7-day cycles of 3% dextran sulfate sodium (DSS) administered through their drinking water to induce colon cancer, or a 7-day administration of 4% DSS to induce colitis. The colorectal tissue was then analyzed for gene expression, histopathology, and immunohistochemistry. Read More

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http://dx.doi.org/10.1016/j.etp.2017.04.002DOI Listing
September 2017
10 Reads

The protective effect of hyperoside on carbon tetrachloride-induced chronic liver fibrosis in mice via upregulation of Nrf2.

Exp Toxicol Pathol 2017 Sep 20;69(7):451-460. Epub 2017 Apr 20.

School of Pharmacy, Guangdong Medical University, Dongguan 523-808, China. Electronic address:

Context: Hyperoside was used to treat cardiovascular disease for many years in China. It was shown great effect on regulation of lipid metabolism. But there is lack of reports about the effects of hyperoside on liver diseases. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09402993163013
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http://dx.doi.org/10.1016/j.etp.2017.04.001DOI Listing
September 2017
7 Reads

In vivo ameliorative effect of cerium oxide nanoparticles in isoproterenol-induced cardiac toxicity.

Exp Toxicol Pathol 2017 Sep 18;69(7):435-441. Epub 2017 Apr 18.

Hormone Evaluation Department, National Organization for Drug Control & Research, Egypt.

Background: Cerium oxide nanoparticles have gained much more attention especially in the field of nanomedicine. This work represents cerium oxide nanoparticles as a new prophylactic model for heart failure progression.

Objective: To investigate the potential protective effect of cerium oxide nanoparticles on Isoproterenol (ISO)-induced cardiac toxicity in rats. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09402993173003
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http://dx.doi.org/10.1016/j.etp.2017.03.001DOI Listing
September 2017
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The Ginkgo biloba extract (GbE) protects the kidney from damage produced by a single and low dose of carbon tetrachloride in adult male rats.

Exp Toxicol Pathol 2017 Sep 14;69(7):430-434. Epub 2017 Apr 14.

Department of Toxicology, Research Center and Advanced Studies of the National Polytechnic Institute, Mexico City, Mexico. Electronic address:

Gingko biloba leaves have been used as herbal medicine in China for 5000 years, and the standardized leaf extract (GB-STE) has some beneficial effects in the treatment of age-related, cardiovascular, and neurological diseases. The aim of this study was to investigate the renoprotective effects of the Gingko biloba extract (GbE) against the toxicity of a single and relatively low dose of carbon tetrachloride (CCl). In male adult Wistar rats, we determined the urine flux, the concentration of total proteins in urine, the concentration of glucose in urine, and the concentration of malondialdehyde (MDA) in renal cortex as well as two markers of renal function (clearance of inulin and p-aminohippurate); we also compared the histological lesions caused by CCl. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09402993163012
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http://dx.doi.org/10.1016/j.etp.2017.04.003DOI Listing
September 2017
21 Reads

Aminoglutethimide-induced lysosomal changes in adrenal gland in mice.

Exp Toxicol Pathol 2017 Sep 11;69(7):424-429. Epub 2017 Apr 11.

Toxicology and Pharmacokinetics Laboratories, Pharmaceutical Research Laboratories, Toray Industries, Inc., 6-10-1, Tebiro, Kamakura, Kanagawa 248-8555, Japan. Electronic address:

Aminoglutethimide is a steroidogenesis inhibitor and inhibits a cholesterol side-chain cleavage enzyme (CYP11A1) that converts cholesterol to pregnenolone in mitochondria. We investigated histopathological changes induced by 5-day administration of AG in mice. Cytoplasmic vacuoles of various sizes and single cell necrosis were found in zona fasciculata cells in AG-treated mice. Read More

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http://dx.doi.org/10.1016/j.etp.2017.04.004DOI Listing
September 2017
6 Reads

Characterization of pancreatic islet cell tumors and renal tumors induced by a combined treatment of streptozotocin and nicotinamide in male SD rats.

Exp Toxicol Pathol 2017 Sep 5;69(7):413-423. Epub 2017 Apr 5.

Laboratory of Veterinary Pathology, Life and Environmental Sciences, Osaka Prefecture University, Rinkuu Ourai Kita 1-58, Izumisano, Osaka 598-8531, Japan.

We herein investigated the histopathological features, including proliferative activity and immunoexpression, of pancreatic islet cell tumors (ICTs) in male SD rats induced by streptozotocin (STZ) and nicotinamide (NA), and discussed their relevance to biological behaviors and prognoses. A total of 70 and 43% of rats developed ICTs 37-45 weeks after the treatment with STZ (50 or 75mg/kg, i.v. Read More

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http://dx.doi.org/10.1016/j.etp.2017.03.007DOI Listing
September 2017
15 Reads

Nicotine promotes cervical carcinoma cell line HeLa migration and invasion by activating PI3k/Akt/NF-κB pathway in vitro.

Exp Toxicol Pathol 2017 Jul 3;69(6):402-407. Epub 2017 Apr 3.

School of Stomatology, Shandong University, PR China; Shandong Provincial Key Laboratory of Oral Tissue Regeneration, PR China. Electronic address:

Cigarette smoking is one of highly risk factors of cervical cancer. Recently nicotine has been reported to increase proliferation and invasion in some smoking related cancers, like non-small cell lung cancer and esophageal squamous cell cancer. However, the effects and mechanisms of nicotine stimulation on cervical cancer cells are not clear. Read More

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http://dx.doi.org/10.1016/j.etp.2017.03.006DOI Listing
July 2017
11 Reads

Phenotypical changes in a differentiating immortalized bronchial epithelial cell line after exposure to mainstream cigarette smoke and e-cigarette vapor.

Exp Toxicol Pathol 2017 Jul 1;69(6):393-401. Epub 2017 Apr 1.

Cultex Laboratories GmbH, Feodor-Lynen-Str. 21, 30625 Hannover, Germany.

3D constructs composed of differentiated immortalized primary normal human bronchial epithelial (NHBE) cells (CL-1548) were repeatedly exposed at the air-liquid interface to non-lethal concentrations of mainstream cigarette smoke (4 cigarettes a day, 5days/week, 8 repetitions in total) and e-cigarette vapor (50 puffs a day, 5 days/week, 8 repetitions in total) to build up a permanent burden on the cells. Samples were taken after 4, 6 and 8 times of repeated smoke exposure and the cultures were investigated using histopathological methods Compared to the clean air-exposed cultures (process control) and incubator control, the aerosol-exposed cultures showed a reduction of ciliated, mucus-producing and club cells. At the end of the exposure phase, we even found metaplastic areas positive for CK13 antibody in the cultures exposed to mainstream cigarette smoke and e-liquid vapor, commonly seen in squamous cells as a marker for non-cornified squamous epithelium. Read More

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http://dx.doi.org/10.1016/j.etp.2017.03.004DOI Listing
July 2017
6 Reads

Graphene oxide nanosheets induced genotoxicity and pulmonary injury in mice.

Exp Toxicol Pathol 2017 Jul 28;69(6):383-392. Epub 2017 Mar 28.

Biotechnology Research Unit, Animal Reproduction Research Institute, Egypt.

Graphene and graphene-related materials have broadly applied in biomedical purposes due to their unique properties, thus safety evaluation of them is crucial. This study was performed to explore the genotoxic and pulmonary toxic potential of different doses of graphene oxide nanosheets' (GOs) in mice.A total of 90 male mature mice were randomly divided into six groups of fifteen mice per each, five groups were intraperitoneally injected by GO at doses of 10, 50, 100, 250 and 500μg/kg b. Read More

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http://dx.doi.org/10.1016/j.etp.2017.03.002DOI Listing
July 2017
10 Reads

The exposure to formaldehyde causes renal dysfunction, inflammation and redox imbalance in rats.

Exp Toxicol Pathol 2017 Jul 21;69(6):367-372. Epub 2017 Mar 21.

Laboratory of Experimental Pathophysiology (LAFEx), Department of Biological Sciences (DECBI), Center of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto (UFOP), Ouro Preto, MG, Brazil. Electronic address:

Twenty-eight Fischer male rats were divided into four groups: control group (CG), exposed to the ambient air, and groups exposed to formaldehyde (FA) at concentrations of 1% (FA1%), 5% (FA5%) and 10% (FA10%). Kidney function was assessed by dosage of uric acid, creatinine and urea. Morphometry was performed on the thickness of the lumen of Bowman's capsule and diameter of the lumen of the renal tubules. Read More

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http://dx.doi.org/10.1016/j.etp.2017.02.008DOI Listing
July 2017
11 Reads

Effects of Artesunate on some biochemical parameters in pregnant albino Wistar rats challenged with lethal strain Plasmodium berghei NK65: Appreciating the activities of artemisinin drugs on key pregnancy hormone balance.

Exp Toxicol Pathol 2017 Jul 21;69(6):408-411. Epub 2017 Mar 21.

Department of Veterinary Physiology, Biochemistry and Pharmacology, Michael Okpara University of Agriculture Umudike, PMB 7267, Umuahia, Abia State, Nigeria. Electronic address:

In humans, malaria in pregnancy can cause serious maternal and foetal morbidity and in extreme untreated cases, foetal mortality occurs. The therapeutic approach to curbing this malaise is the administration of an effective and/or combinations of anti-malaria medicaments. Acute or chronic administration of some of these drugs, however, gives rise to some adverse medical conditions including reproductive dysfunction, especially in pregnancy. Read More

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http://dx.doi.org/10.1016/j.etp.2017.02.005DOI Listing
July 2017
10 Reads

Curcuma longa Linn. extract and curcumin protect CYP 2E1 enzymatic activity against mercuric chloride-induced hepatotoxicity and oxidative stress: A protective approach.

Exp Toxicol Pathol 2017 Jul 21;69(6):373-382. Epub 2017 Mar 21.

School of Pharmacy and Technology Management (SPTM), Shri Vile Parle Kelavani Mandal's (SVKM's),Narsee Monjee Institute of Management Studies (NMIMS), Mukesh Patel Technology Park, Babulde, Bank of Tapi River, Mumbai-Agra Road, Shirpur, Dist. Dhule-India.

The present investigation has been conducted to evaluate the therapeutic potential of Curcuma longa (200mgkg, po) and curcumin (80mgkg, po) for their hepatoprotective efficacy against mercuric chloride (HgCl: 12μmolkg, ip; once only) hepatotoxicity. The HgCl administration altered various biochemical parameters, including transaminases, alkaline phosphatase, lactate dehydrogenase, bilirubin, gamma-glutamyl transferase, triglycerides and cholesterol contents with a concomitant decline in protein and albumin concentration in serum which were restored towards control by therapy of Curcuma longa or curcumin. On the other hand, both treatments showed a protective effect on drug metabolizing enzymes viz. Read More

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http://dx.doi.org/10.1016/j.etp.2017.02.009DOI Listing
July 2017
27 Reads
2.005 Impact Factor

Improvement of the CULTEX exposure technology by radial distribution of the test aerosol.

Exp Toxicol Pathol 2017 Jul 3;69(6):359-365. Epub 2017 Mar 3.

Vor der Hardt 16, 57392 Oberkirchen, Germany.

The exposure of cellular based systems cultivated on microporous membranes at the air-liquid interface (ALI) has been accepted as an appropriate approach to simulate the exposure of cells of the respiratory tract to native airborne substances. The efficiency of such an exposure procedure with regard to stability and reproducibility depends on the optimal design at the interface between the cellular test system and the exposure technique. The actual exposure systems favor the dynamic guidance of the airborne substances to the surface of the cells in specially designed exposure devices. Read More

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http://dx.doi.org/10.1016/j.etp.2017.02.004DOI Listing
July 2017
7 Reads

Cytotoxicity and genotoxicity effects of arsenic trioxide on SQ20B human laryngeal carcinoma cells.

Exp Toxicol Pathol 2017 Jul 3;69(6):349-358. Epub 2017 Mar 3.

Normandie Univ, UNICAEN, ABTE, 14000 Caen, France; Centre François Baclesse, Avenue Général Harris, BP5026, F-14076 Caen Cedex-05, France.

This study investigates the cytotoxicity and the genotoxicity induced by arsenic trioxide AsOin human laryngeal SQ20B carcinoma cell line. SQ20B cells were exposed to graded concentrations of arsenic trioxide (2 and 5μM) for 48h. Comet assay and γ-H2AX foci formation were used for measuring DNA damages, flow cytometry was used to identify cell cycle alterations and apoptosis, while cell morphology was visualized using transmission electron microscopy. Read More

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http://dx.doi.org/10.1016/j.etp.2017.02.003DOI Listing
July 2017
6 Reads

Sulfated glycosaminoglycans in bladder tissue and urine of rats after acute exposure to paraoxon and cyclophosphamide.

Exp Toxicol Pathol 2017 Jul 1;69(6):339-347. Epub 2017 Mar 1.

Research Institute of Hygiene, Occupational Pathology and Human Ecology, Saint Petersburg, Russia; Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences, Saint Petersburg, Russia. Electronic address:

Glycosaminoglycans (GAGs) in the urine of Wistar rats, and on the surface of the epithelium and lamina propria of the bladder, were quantitatively assessed before and after acute intoxication with paraoxon or cyclophosphamide. Paraoxon was administered subcutaneously (s.c. Read More

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http://dx.doi.org/10.1016/j.etp.2017.02.007DOI Listing
July 2017
16 Reads

Maternal exposure to titanium dioxide nanoparticles during pregnancy and lactation alters offspring hippocampal mRNA BAX and Bcl-2 levels, induces apoptosis and decreases neurogenesis.

Exp Toxicol Pathol 2017 Jul 27;69(6):329-337. Epub 2017 Feb 27.

Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Introduction: The usage of Titanium dioxide nanoparticles (TiO-NPs) covers a vast area in different fields ranging from cosmetics and food to the production of drugs. Maternal exposure to TiO-NPs during developmental period has been associated with hippocampal injury and with a decrease in learning and memory status of the offspring. However, little is known about its injury mechanism. Read More

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http://dx.doi.org/10.1016/j.etp.2017.02.006DOI Listing
July 2017
12 Reads

Metaplastic phenotype in human primary bronchiolar epithelial cells after repeated exposure to native mainstream smoke at the air-liquid interface.

Exp Toxicol Pathol 2017 Jun 21;69(5):307-315. Epub 2017 Feb 21.

Cultex Laboratories GmbH, Feodor-Lynen-Str. 21, 30625, Hannover, Germany.

3D constructs composed of primary normal differentiated human bronchiolar epithelial (NHBE) cells as mono- or co-culture in combination with normal human lung fibroblasts were exposed repeatedly at the air-liquid interface with non-lethal concentrations of mainstream cigarette smoke (4 cigarettes a day, 5days/week, 13 times repetition in total) to build up a permanent burden on the cells. Samples were taken after 4, 8 and 13 times of repeated smoke exposure and the cultures were analyzed by histopathological methods In comparison with the clean air exposure (process control) and incubator control cells the cigarette smoke exposed cultures showed a reduction of cilia bearing as well as mucus producing cells. In both mono- as well as co-cultures, hyperplasia was induced showing different histological cell types (undifferentiated secretory and squamous cell types). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09402993163022
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http://dx.doi.org/10.1016/j.etp.2017.01.015DOI Listing
June 2017
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Protective effects of silymarin and curcumin on cyclophosphamide-induced cardiotoxicity.

Exp Toxicol Pathol 2017 Jun 21;69(5):317-327. Epub 2017 Feb 21.

Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Adnan Menderes, 09016 Aydin, Turkey.

Introduction: Cyclophosphamide (CP) is a potent anticancer agent; its clinical use is limited due to its marked cardiotoxicity.

Aim: The present study was aimed at evaluating the cardioprotective effects of silymarin (SLY) and curcumin (CUR), which have strong antioxidant properties, against the toxic effects of high-dose CP on the heart of rats.

Materials And Methods: A total of 36 adult Wistar albino female rats were randomly divided into six groups. Read More

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http://dx.doi.org/10.1016/j.etp.2017.02.002DOI Listing
June 2017
6 Reads

Cytogenotoxicological defense of retinyl palmitate in the front damage of antineoplastics.

Exp Toxicol Pathol 2017 Jun 16;69(5):293-297. Epub 2017 Feb 16.

Laboratory of Toxicology and Genetics, Federal University of Piauí, Teresina, Piaui, 64.049-550, Brazil; Postgraduate Program in Biotechnology (RENORBIO), Federal University of Piauí, Teresina, Piauí, 64.049-550, Brazil; Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, Piauí, 64.049-550, Brazil.

Cancer, the multifactorial pathology and to date is the most lethal causes of death in the world. Cyclophosphamide (CPA) and doxorubicin (DOX) are the individually or combindly used two anticancer drugs. The antineoplastic drugs-mediated genetic instability can be overcome by using antioxidants. Read More

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http://dx.doi.org/10.1016/j.etp.2017.01.013DOI Listing
June 2017
52 Reads