4,058 results match your criteria Experimental and Molecular Pathology [Journal]


Ailanthone up-regulates miR-449a to restrain acute myeloid leukemia cells growth, migration and invasion.

Exp Mol Pathol 2019 Apr 16. Epub 2019 Apr 16.

Department of Hepatobiliary Surgery, Jining No.1 People's Hospital, Jining 272000, Shandong, China. Electronic address:

Background: Ailanthone (AIL) is a quassinoid isolated from traditional Chinese herbal medicine Ailanthus altissima. The anti-tumor activities of AIL have been reported in various solid tumors. This study aimed to reveal the in vitro effect of AIL on acute myeloid leukemia (AML) cells. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.04.011DOI Listing

Intracerebral matrix metalloproteinase 9 in fatal diabetic ketoacidosis.

Exp Mol Pathol 2019 Apr 12. Epub 2019 Apr 12.

Department of Neurology, University of Maryland, School of Medicine, Baltimore, MD, USA. Electronic address:

There is increasing awareness that in addition to the metabolic crisis of diabetic ketoacidosis (DKA) caused by severe insulin deficiency, the immune inflammatory response is likely an active multicomponent participant in both the acute and chronic insults of this medical crisis, with strong evidence of activation for both the cytokine and complement system. Recent studies report that the matrix metalloproteinase enzymes and their inhibitors are systemically activated in young Type 1 diabetes mellitus (T1D) patients during DKA and speculate on their involvement in blood-brain barrier (BBB) disruption. Based on our previous studies, we address the question if matrix metalloproteinase 9 (MMP9) is expressed in the brain in the fatal brain edema (BE) of DKA. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.04.008DOI Listing

miR-186-5p promotes cell growth, migration and invasion of lung adenocarcinoma by targeting PTEN.

Exp Mol Pathol 2019 Apr 11. Epub 2019 Apr 11.

Department of Thoracic Surgery, Peking University China, Japan Friendship School of Clinical Medicine, Beijing 100029, China. Electronic address:

Objective: To explore the expression of miR-186-5p in lung adenocarcinoma (LUAD) and its possible function associated with cancer cell proliferation, migration and invasion.

Methods: MiR-186-5p expression levels in LUAD samples, human LUAD cell lines H1299 and NCI-H1975, and normal human lung epithelial cell line BEAS-IB were assessed by quantitative real-time PCR (qRT-PCR). H1299 and NCI-H1975 cells were transfected with miR-186-5p mimic or miRNA negative control. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144800183058
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http://dx.doi.org/10.1016/j.yexmp.2019.04.007DOI Listing
April 2019
1 Read

Triptolide inhibits viability and migration while promotes apoptosis in nephroblastoma cells by regulation of miR-193b-3p.

Exp Mol Pathol 2019 Apr 9;108:80-88. Epub 2019 Apr 9.

Department of Pediatric Surgery, Jining No.1 People's Hospital, Jining 272011, China. Electronic address:

Background: Triptolide (TPL) is a potential anti-tumor natural compound. However, its role in nephroblastoma is poorly studied. Herein, we aimed to reveal the regulatory effects of TPL on human nephroblastoma cells (G-401 and WiT49) as well as the regulatory mechanism in G-401 cells. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144800183042
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http://dx.doi.org/10.1016/j.yexmp.2019.04.006DOI Listing
April 2019
1 Read

Anti-proliferation and anti-metastatic effects of sevoflurane on human osteosarcoma U2OS and Saos-2 cells.

Exp Mol Pathol 2019 Apr 8. Epub 2019 Apr 8.

Department of Anesthesiology, Huai'an First People's Hospital of Nanjing Medical University, Huai'an 223300, China. Electronic address:

Sevoflurane is a commonly used anesthetic in cancer surgery. However, little information is available about the effects of sevoflurane on the osteosarcoma cells. The present study explored the effects of sevoflurane on the proliferation, migration and invasion of osteosarcoma cells, as well as the underlying mechanism. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.04.005DOI Listing

Immune profiling reveals the diverse nature of the immune response in NSCLC and reveals signaling pathways that may influence the anti-tumor immune response.

Exp Mol Pathol 2019 Apr 3. Epub 2019 Apr 3.

GoPath Laboratories, 1351 Barclay Blvd, Buffalo Grove, IL 60089, United States of America; CorePath Laboratories, 2020 Babcock Rd, Suite 30, San Antonio, TX 78229, United States of America.

Recent FDA approvals of immunotherapy for NSCLC provide patients new treatment options, and these approvals also highlight the importance of the immune response in cancer treatment. While immunotherapy provides patients a new treatment option, the therapy is effective in less than half of the treated patients. To attain greater insight into the tumor-immune microenvironment, NSCLC tumors were analyzed by IHC and RNA-seq. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.04.004DOI Listing

Concise approach for screening long non-coding RNAs functionally linked to human breast cancer associated genes.

Exp Mol Pathol 2019 Apr 3;108:89-96. Epub 2019 Apr 3.

Department of Biochemistry, BK21 Plus and Research Institute for Veterinary Science, School of Veterinary Medicine, Seoul National University, Seoul, South Korea. Electronic address:

Cancer research studies using next-generation sequencing have revealed a number of genes of which aberrant expression is associated with various cancers. Recently, long non-coding RNA (lncRNA) has been highlighted due to its tissue-specific expression and cell cancerization functions, such as the regulation of key tumor suppressors. In this study, we suggest a very efficient approach to survey lncRNAs putatively associated with breast cancer. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.04.003DOI Listing
April 2019
2 Reads

The different expression of tumor suppressors, RASSF1A, RUNX3, and GSTP1, in patients with alcoholic Steatohepatitis (ASH) vs non-alcoholic Steatohepatitis (NASH).

Exp Mol Pathol 2019 Apr 2. Epub 2019 Apr 2.

Harbor-UCLA Medical Center, Department of Pathology, Torrance, CA 90502, United States. Electronic address:

As the fifth most common cancer and the second leading cause of cancer related deaths worldwide, hepatocellular carcinoma (HCC) causes up to one million deaths annually. Alcoholic steatohepatitis (ASH) and non-alcoholic steatohepatitis (NASH) are becoming the two major risk factors because both may develop liver fibrosis and hepatocellular carcinoma (HCC) if left untreated. However, compared with 3-10% of patients with ASH may progress to HCC annually, about only 0. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.04.002DOI Listing
April 2019
5 Reads

Plakophilin 1 is methylated and has a tumor suppressive activity in human lung cancer.

Exp Mol Pathol 2019 Apr 1;108:73-79. Epub 2019 Apr 1.

Section Pathology of the Institute of Forensic Medicine, University Hospital Jena, Friedrich Schiller University Jena, Am Klinikum 1, 07747 Jena, Germany. Electronic address:

Background: Plakophilin 1 (PKP1) is an important plaque component of desmosomes, major intercellular adhesive junctions that act as anchorage points for intermediate filaments. Abnormal expression of PKP1 was observed in various types of cancer, however so far its function in lung cancer has not yet been elucidated.

Methods: The expression of PKP1 was analyzed by RT-PCR and western blotting in lung cancer cell lines. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.04.001DOI Listing
April 2019
2 Reads

High-resolution melting analysis to screen the ST18 gene functional risk variant for pemphigus vulgaris: The occasion to open a debate on its usefulness in clinical setting.

Exp Mol Pathol 2019 Mar 26;108:57-63. Epub 2019 Mar 26.

UOC Laboratory Medicine, Genetics and Toxicology, IDI IRCCS, Fondazione Luigi Maria Monti, Via Monti di Creta 104, Rome, Italy; Polo Scienze per Immagini, di Laboratorio e Infettivologiche, "Fondazione Policlinico Agostino Gemelli" - IRCCS, Rome, Italy. Electronic address:

The ST18 -497-65050 T > C polymorphisms (rs17315309) exhibit a very strong association in the pathogenesis of Pemphigus Vulgaris (PV) and could represent a new potential molecular target for the treatment of disease. The present study aimed to establish a low-cost, sensitive and reliable assay using high-resolution melting curve analysis (HRMA) on magnetic induction rotor-based platform, the Magnetic Induction Cycler (MIC) (Bio molecular Systems). HRMA assay was able to identify easily and unambiguously the c. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.03.009DOI Listing
March 2019
2.706 Impact Factor

Role of the inflammasomes in HIV-associated neuroinflammation and neurocognitive disorders.

Exp Mol Pathol 2019 Mar 26;108:64-72. Epub 2019 Mar 26.

Department of Neurology, University of Maryland, Baltimore, MD 21201, United States of America; VA Medical Center, Baltimore, MD 21201, United States of America. Electronic address:

HIV associated neurocognitive disorders (HAND) is a unique form of neurological impairment that stems from HIV. This disease and its characteristics can be accredited to incorporation of DNA and mRNA of HIV-1 into the CNS. A proper understanding of the intricacies of HAND and the underlying mechanisms associated with corresponding immune reactions are vital for the potential development of a reliable treatment for HAND. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.03.008DOI Listing
March 2019
2 Reads

Tenascin-C is involved in promotion of cancer stemness via the Akt/HIF1ɑ axis in esophageal squamous cell carcinoma.

Exp Mol Pathol 2019 Mar 20. Epub 2019 Mar 20.

Institute for Regenerative Medicine, Yanbian University College of Medicine, Yanji, China; Department of Pathology, Yanbian University College of Medicine, Yanji, China. Electronic address:

Although tenascin-C (TNC), an extracellular matrix protein, has been shown to be widely expressed in stromal fibroblasts in various cancers, the role of its expression in esophageal squamous cell carcinoma (ESCC) cells remains unclear. Using immunohistochemistry, we investigated the expression of cancer stem-like cell (CSC) markers, epithelial-to-mesenchymal transition (EMT)-related genes, and the Akt/hypoxia-inducible factor-1α (HIF1α) signal pathway in ESCC tissue specimens from 154 patients. We further addressed the effects of TNC on the Akt/HIF1α axis and its putative association with cancer stemness in several ESCC cell lines by immunofluorescence imaging and western blot analysis. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.03.007DOI Listing
March 2019
1 Read

Focal adhesion kinase coordinates costamere-related JNK signaling with muscle fiber transformation after Achilles tenotomy and tendon reconstruction.

Exp Mol Pathol 2019 Mar 15;108:42-56. Epub 2019 Mar 15.

Laboratory for Muscle Plasticity, Department of Orthopedics, University of Zurich, Balgrist Campus, Zurich, Switzerland. Electronic address:

Achilles tendon rupture necessitates rapid tendon reattachment to reinstate plantar flexion before affected muscles deteriorate through muscle fiber atrophy and transformation. The implicated process may involve alterations in sarcolemmal sites of myofibril attachment (costameres), which control myofibrillogenesis via a mechano-regulated mechanism through integrin-associated focal adhesion kinase (FAK). We assessed the contribution of FAK to alterations in fiber type composition and expression of costamere-associated structural proteins, the phosphorylation status of Y397-FAK and downstream mTOR/JNK-P70S6K hypertrophy signaling in rat soleus muscle after Achilles tenotomy and tendon repair. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.03.006DOI Listing
March 2019
2 Reads

Overexpression of survivin in pediatric Hodgkin lymphoma tumor cells: Characterization of protein expression and splice-variants transcription profile.

Exp Mol Pathol 2019 Mar 12;108:24-31. Epub 2019 Mar 12.

Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas (IMIPP), CONICET-GCBA, División Patología, Hospital de Niños Ricardo Gutiérrez, Buenos Aires, Argentina.

Survivin is abundantly expressed during fetal development but absent in most differentiated adult tissues; an exception being components of the immune system, such as B and T lymphocytes. Beyond acting as a master regulator of the cell cycle, survivin acts as an inhibitor of apoptosis and is overexpressed in almost all carcinoma types; however, its expression in lymphomas is lesser-explored. Survivin's role in carcinogenesis was subjected to its sub-cellular localization and splice transcripts expression, namely wild-type survivin, survivin-∆Ex3 and survivin-2B. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.03.005DOI Listing

TRAF4 promotes endometrial cancer cell growth and migration by activation of PI3K/AKT/Oct4 signaling.

Exp Mol Pathol 2019 Mar 7;108:9-16. Epub 2019 Mar 7.

Department of Gynecology, Jining No.1 People's Hospital, Jining 272011, China. Electronic address:

Objective: Endometrial cancer (EC) is ranked as the most common gynecologic malignancy of the female genital tract and the fourth most common neoplasia in women. Accumulated evidences reveal that TRAF4 plays a critical role in the progress of various cancers, but its functions in EC remains unclear. This study aimed to explore the role and mechanism of TRAF4 in EC progress. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.03.003DOI Listing
March 2019
2 Reads
2.706 Impact Factor

Ginsenoside Rh2 inhibits proliferation but promotes apoptosis and autophagy by down-regulating microRNA-638 in human retinoblastoma cells.

Exp Mol Pathol 2019 Mar 7;108:17-23. Epub 2019 Mar 7.

Department of Ophtalmology, Linyi Central Hospital, Linyi, Shandong 276400, China. Electronic address:

Even though recent therapeutic advances make retinoblastoma (RB) becomes a curable tumor, the outcome of long-term survival is poor due to the risk of developing secondary tumors. We aimed to explore the functional role of Ginsenoside Rh2 (GRh2) in human RB cells and the underlying mechanisms. Human RB Y79 and RBL-13 cells were incubated with or without GRh2, followed by assessments of cell viability, proliferation and apoptosis using Cell Counting Kit-8, bromodeoxyuridine, and flow cytometry assays. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.03.004DOI Listing

Quercetin ameliorates lipopolysaccharide-caused inflammatory damage via down-regulation of miR-221 in WI-38 cells.

Exp Mol Pathol 2019 Mar 6;108:1-8. Epub 2019 Mar 6.

Children's Medical Center, The Affiliated Hospital of Qingdao University, Qingdao 266000, China.

Background: Pneumonia is a common respiratory disease in pediatrics. Quercetin is a natural flavonoid widely distributed in many foods and drinks. Herein, we focused our investigation on the possible protective activity of quercetin in lipopolysaccharide (LPS)-caused inflammatory damage of WI-38 lung fibroblasts. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144800183055
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http://dx.doi.org/10.1016/j.yexmp.2019.03.002DOI Listing
March 2019
4 Reads

Down-regulation of microRNA-31-5p inhibits proliferation and invasion of osteosarcoma cells through Wnt/β-catenin signaling pathway by enhancing AXIN1.

Exp Mol Pathol 2019 Mar 4;108:32-41. Epub 2019 Mar 4.

Department of Clinical Laboratory, The Second Hospital of Jilin University, Changchun 130041, China. Electronic address:

Objectives: Recently, the role of microRNA-31-5p (miR-31-5p) in gene expression regulation has been reported in various cancers. Studies have shown that Wnt/β-catenin signaling pathway is involved in the proliferation and invasion of osteosarcoma (OS) cells. Therefore, this study aims to probe into the regulatory role of miR-31-5p targeting AXIN1 in OS cells through Wnt/β-catenin signaling pathway. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.03.001DOI Listing
March 2019
1 Read

Primary sclerosing epithelioid fibrosarcoma of the kidney: Report of two additional cases with a clinicopathological and molecular cytogenetic study.

Exp Mol Pathol 2019 04 27;107:179-183. Epub 2019 Feb 27.

Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai 200032, China.

We present two cases of sclerosing epithelioid fibrosarcoma (SEF) that arose primarily in the kidney. The tumor in both cases was located at the upper pole of the kidney. Clincially, they were suspected as renal cell carcinomas. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.02.006DOI Listing
April 2019
2 Reads

Ketamine inhibits colorectal cancer cells malignant potential via blockage of NMDA receptor.

Exp Mol Pathol 2019 04 25;107:171-178. Epub 2019 Feb 25.

Department of Anaesthesiology, Xinjiang Medical University, Affiliated Tumour Hospital, Xinjiang, PR China. Electronic address:

Ketamine, a common N-methyl-d-aspartate receptor (NMDAR) antagonist, is an option for cancer pain treatment in clinical practice. Ketamine has been shown to have the capacity to attenuate cancer cells malignancy. However, the underlying mechanism remains elusive. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.02.004DOI Listing

Epitope recognized by anti-glomerular basement membrane (GBM) antibody in a patient with repeated relapse of anti-GBM disease.

Exp Mol Pathol 2019 04 25;107:165-170. Epub 2019 Feb 25.

Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Sapporo, Japan. Electronic address:

The major epitopes recognized by autoantibodies in anti-glomerular basement membrane (GBM) disease are found in the α3-subunit non-collagenous domain of type IV collagen [α3(IV)NC1], which is present in the glomerular and alveolar basement membranes. These epitopes are structurally cryptic, owing to the hexamer formation of the non-collagenous domain of α3, α4, and α5 subunits and are expressed by the dissociation of the hexamer. Anti-GBM disease usually manifests as a single attack (SA), and we rarely see patients who repeatedly relapse. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.02.005DOI Listing
April 2019
1 Read

GLP-1 receptor agonist impairs keratinocytes inflammatory signals by activating AMPK.

Exp Mol Pathol 2019 04 15;107:124-128. Epub 2019 Feb 15.

Department of Dermatology and Venerology,Chongqing Three Gorges Central Hospital, ChongQing 404100, China.

Purpose: Liraglutide, a glucagon-like peptide-1 (GLP-1) analogue, is an antidiabetic drug. It has been shown to improve the Psoriasis Area and Severity Index (PASI) in patients with type 2 diabetes and psoriasis in clinical practice, but the mechanism remains somewhat unclear. We used lipopolysaccharides (LPS) to induce inflammatory response in keratinocytes and explore the mechanism. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.01.014DOI Listing
April 2019
1 Read
2.706 Impact Factor

p38 MAP kinases: plausible diagnostic and prognostic serum protein marker of non small cell lung cancer.

Exp Mol Pathol 2019 04 14;107:118-123. Epub 2019 Feb 14.

Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India. Electronic address:

Introduction: p38 MAPK signaling molecules plays a dual role in cancer, both progression and suppression. Elevated expression of p38α was reported in lung cancer tissue in rat model. Our objective was to explore the concentration of all 4 isoforms of p38MAPK in serum of Non Small Cell Lung Cancer (NSCLC). Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.01.009DOI Listing
April 2019
5 Reads
2.706 Impact Factor

The clinical significance of endothelin receptor type B in hepatocellular carcinoma and its potential molecular mechanism.

Exp Mol Pathol 2019 04 12;107:141-157. Epub 2019 Feb 12.

Department of Pathology, First Affiliated Hospital of Guangxi Medical University, No. 6 Shuangyong Road, Nanning, Guangxi Zhuang Autonomous Region 530021, PR China. Electronic address:

Objective: To explore the clinical significance and potential molecular mechanism of endothelin receptor type B (EDNRB) in hepatocellular carcinoma (HCC).

Methods: Immunohistochemistry was used to detect EDNRB protein expression level in 67 HCC paraffin embedded tissues and adjacent tissues. Correlations between EDNRB expression level and clinicopathologic parameters were analyzed in our study. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.02.002DOI Listing
April 2019
4 Reads

miR-26b modulates OA induced BMSC osteogenesis through regulating GSK3β/β-catenin pathway.

Exp Mol Pathol 2019 04 13;107:158-164. Epub 2019 Feb 13.

Department of Orthopedics, Beijing Northern Hospital, China North Industries, Beijing 100089, PR.China.; Inner Mongolia Medical University, Hohhot 014010, Inner Mongolia, PR.China.. Electronic address:

Backgrounds: Osteoactivin (OA) is a key regulator promoting bone marrow stromal cells osteogenesis progress, while Dexamethasone (Dex) could inhibit OA induced osteogenesis and lead to osteoporosis. miR-26b increased during BMSC osteogenesis but whether it participates in this progress is enigma. Osteogenesis is under regulation of canonical Wnt signaling pathway which could serve as potential target for miR-26b. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.02.003DOI Listing
April 2019
2 Reads

Proteomic characterization of early lung response to breast cancer metastasis in mice.

Exp Mol Pathol 2019 04 11;107:129-140. Epub 2019 Feb 11.

Jagiellonian University, Jagiellonian Centre for Experimental Therapeutics (JCET), Bobrzynskiego 14, 30-348 Krakow, Poland; Jagiellonian University Medical College, Faculty of Pharmacy, Department of Toxicology, Medyczna 9, 30-688 Krakow, Poland. Electronic address:

Introduction: The tumor-promoting rearrangement of the lungs facilitates the process of cancer cell survival in a foreign microenvironment and enables their protection against immune defense. The study aimed to define the fingerprint of the early rearrangement of the lungs via the proteomic profiling of the lung tissue in the experimental model of tumor metastasis in a murine 4T1 mammary adenocarcinoma.

Materials And Methods: The studies were performed on 7-8-week-old BALB/c female mice. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.02.001DOI Listing

High levels of RAI3 expression is linked to shortened survival in esophageal cancer patients.

Exp Mol Pathol 2019 04 29;107:51-56. Epub 2019 Jan 29.

General, Visceral and Thoracic Surgery Department and Clinic, University Medical Center Hamburg-Eppendorf, Germany. Electronic address:

Expression of the retinoic acid-induced protein 3 (RAI3) has been suggested to predict clinical outcome in a variety of malignancies. However, its role in esophageal cancers remains unclear. Immunohistochemical RAI3 staining was analyzed on tissue microarrays containing 359 esophageal adenocarcinomas (EAC) and 254 esophageal squamous cell carcinomas (ESCC). Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.01.013DOI Listing

LncRNA KCNQ1OT1 promoted BMP2 expression to regulate osteogenic differentiation by sponging miRNA-214.

Exp Mol Pathol 2019 04 28;107:77-84. Epub 2019 Jan 28.

Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha 410008, PR China. Electronic address:

Background: Osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is of much significance for bone formation, the imbalance of it would result in osteoporosis and other pathological bone defects. Increasing evidences showed that long non-coding RNAs (lncRNAs) and miRNAs played vital roles in the regulation of osteogenic differentiation. LncRNA KCNQ1OT1 was often regarded as an imprinted lncRNA and was related to tumor progression, while its function in osteogenic differentiation remained unclear. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144800183047
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http://dx.doi.org/10.1016/j.yexmp.2019.01.012DOI Listing
April 2019
6 Reads

Propofol suppresses proliferation and migration of papillary thyroid cancer cells by down-regulation of lncRNA ANRIL.

Exp Mol Pathol 2019 04 28;107:68-76. Epub 2019 Jan 28.

Department of Anesthesiology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China. Electronic address:

Background: Propofol is a popular anesthetic agent, with potent anti-tumor activity against many cancers. The objective of this study was to explore the potential effect of propofol on papillary thyroid cancer (PTC) in vitro.

Methods: Human PTC cell lines TPC-1 and IHH-4 were treated by propofol. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144800183032
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http://dx.doi.org/10.1016/j.yexmp.2019.01.011DOI Listing
April 2019
9 Reads

Silence of lncRNA CHRF protects H9c2 cells against lipopolysaccharide-induced injury via up-regulating microRNA-221.

Exp Mol Pathol 2019 04 26;107:43-50. Epub 2019 Jan 26.

Department of Pediatrics, Jining No.1 People's Hospital, Jining 272011, China. Electronic address:

Background: Cardiac hypertrophy related factor (CHRF), a newly recognized long non-coding RNA (lncRNA), is a central regulator in cardiac hypertrophy responses. This study attempted to show the potential role of lncRNA CHRF in bacterial infection caused myocarditis.

Methods: H9c2 cells were transfected with small interfering RNAs (siRNAs) specific for lncRNA CHRF alone or in combination with miR-221 inhibitor, and then subjected to lipopolysaccharide (LPS). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144800183042
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http://dx.doi.org/10.1016/j.yexmp.2019.01.010DOI Listing
April 2019
12 Reads
2.706 Impact Factor

Triptolide inhibits the growth and migration of colon carcinoma cells by down-regulation of miR-191.

Authors:
Yuxi Qi Jinliang Li

Exp Mol Pathol 2019 04 23;107:23-31. Epub 2019 Jan 23.

Department of Anorectal Surgery, Jining No.1 People's Hospital, Jining 272011, China. Electronic address:

Background: Triptolide (TPL) is the active component of Tripterygium wifordii Hook F, which has been reported to exert anti-tumor efficacies. Herein, we aimed to examine the efficacy of TPL in colorectal cancer (CRC) cells and to reveal its underlying mechanisms.

Methods: Human CRC cell lines HT-29 and SW480 were treated by TPL for 24 h in the presence or absence of epithelial-to-mesenchymal transition (EMT) inducer TGF-β1. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144800183048
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http://dx.doi.org/10.1016/j.yexmp.2019.01.008DOI Listing
April 2019
7 Reads

Silencing of the Na/H exchanger 1(NHE-1) prevents cardiac structural and functional remodeling induced by angiotensin II.

Exp Mol Pathol 2019 04 18;107:1-9. Epub 2019 Jan 18.

Centro de investigaciones Cardiovasculares "Dr. Horacio E, Cingolani", Facultad de Ciencias Médicas UNLP-CONICET, Argentina. Electronic address:

Chronic activation of the renin angiotensin system (RAS) favors several cardiac diseases, among which myocardial hypertrophy occupies an outstanding place. In this context, the hyperactivity of the cardiac Na/H (NHE-1) exchanger plays a key role. The pathologic remodeling of the myocardium constitutes an independent risk factor for morbidity and mortality with continuously increasing healthcare cost. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.01.007DOI Listing
April 2019
2 Reads

The anti-malarial drug artesunate causes cell cycle arrest and apoptosis of triple-negative MDA-MB-468 and HER2-enriched SK-BR-3 breast cancer cells.

Exp Mol Pathol 2019 04 17;107:10-22. Epub 2019 Jan 17.

Department of Pathology, Dalhousie University, Halifax, Canada; Department of Microbiology and Immunology, Dalhousie University, Halifax, Canada; Department of Surgery, Dalhousie University, Halifax, Canada. Electronic address:

Breast cancer is the most prevalent cancer diagnosis in women, with triple-negative and human epidermal growth factor 2 (HER2)-enriched advanced breast cancers having the poorest prognoses. The morbidity and mortality associated with advanced disease, as well as the emergence of multi-drug resistant variants, highlights the urgency to develop novel therapeutic agents. Artesunate (ART) is a semi-synthetic derivative of artemisinin from the Chinese herb sweet wormwood. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.01.006DOI Listing
April 2019
3 Reads

Aluminum disrupts the prenatal development of the male and female gerbil prostate (Meriones unguiculatus).

Exp Mol Pathol 2019 04 17;107:32-42. Epub 2019 Jan 17.

Department of Histology, Embryology and Cell Biology, Laboratory of Microscopy Applied to Reproduction, Institute of Biological Sciences, Federal University of Goiás, Goiânia, Goiás 74001970, Brazil. Electronic address:

Normal prostate development is highly dependent of an equilibrated hormonal regulation, so that sensible interferences during this period may predispose the gland to lesions during aging. Industrial activities have increased the exposure of this gland to active elements found in environment, such as aluminum (Al). Al presents toxic effect for living beings, having the potential to disrupt the development and growth of several organs and systems. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.01.005DOI Listing
April 2019
2 Reads

Effects of GST null genotypes on individual susceptibility to leukemia: A meta-analysis.

Exp Mol Pathol 2019 Jan 16. Epub 2019 Jan 16.

Department of Hematology, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215000, China; Institute of Blood and Marrow Transplantation, Collaborative Innovation Center of Hematology, Soochow University, Suzhou 215000, China; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Soochow University, Suzhou 215000, China. Electronic address:

Background: Whether glutathione S-transferases (GST) null genotypes influence individual susceptibility to leukemia remains controversial. Thus, we performed this meta-analysis to better analyze potential influences of GST null genotypes on individual susceptibility to leukemia.

Methods: Literature retrieve was conducted in PubMed, Web of Science and Embase. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.01.004DOI Listing
January 2019
3 Reads

Ginsenoside Rg3 inhibits cell growth, migration and invasion in Caco-2 cells by downregulation of lncRNA CCAT1.

Authors:
Jinliang Li Yuxi Qi

Exp Mol Pathol 2019 02 8;106:131-138. Epub 2019 Jan 8.

Department of Anorectal Surgery, Jining No.1 People's Hospital, Jining 272011, China. Electronic address:

Background: Colorectal cancer (CRC) is a troublesome disease with high morbidity and mortality. Ginsenoside Rg3 possesses anti-cancer properties. Colon Cancer Associated Transcript 1 (CCAT1) participates in the genesis, development, invasion and metastasis of colorectal cancer. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.01.003DOI Listing
February 2019
3 Reads

Baicalin protects human retinal pigment epithelial cell lines against high glucose-induced cell injury by up-regulation of microRNA-145.

Exp Mol Pathol 2019 02 6;106:123-130. Epub 2019 Jan 6.

Department of Ophthalmology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China. Electronic address:

Background: Diabetic retinopathy (DR) is a common complication of diabetes mellitus, which is a major reason of blindness. Baicalin (BAI) is a flavonoid extracted from Scutellaria baicalensis, whose pharmacological characterizes have been widely reported in various diseases. However, it remains unclear the effect of BAI on DR. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144800183034
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http://dx.doi.org/10.1016/j.yexmp.2019.01.002DOI Listing
February 2019
8 Reads

ZEB1 mediates doxorubicin (Dox) resistance and mesenchymal characteristics of hepatocarcinoma cells.

Exp Mol Pathol 2019 02 4;106:116-122. Epub 2019 Jan 4.

Interventional Vascular Surgery, Hunan Provincial People's Hospital, No. 61 Jiefangxi Road, Changsha 410005, China. Electronic address:

The acquired chemoresistance during long term chemotherapy is one of the most important factors to limit the application of Doxorubicin (Dox) on clinical treatment of hepatocellular carcinoma (HCC) patients. Our present study found that Dox resistant HCC (HCC/Dox) cells had greater capability of in vitro migration and invasion compared to their parental cells. HCC/Dox cells exhibited mesenchymal characteristics, which was evidenced by the up regulation of fibronectin, vimentin while down regulation of E-Cadherin. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.01.001DOI Listing
February 2019
2 Reads

Brazilein induces apoptosis and G1/G0 phase cell cycle arrest by up-regulation of miR-133a in human vestibular schwannoma cells.

Exp Mol Pathol 2019 04 2;107:95-101. Epub 2019 Jan 2.

Department of Chinese Medicine, Yantai Hospital of Traditional Chinese Medicine, Yantai 264000, China. Electronic address:

Background: Acoustic neuroma is a benign and usually slow growing tumor. Brazilein is a natural compound extracted from hematoxylin. However, there has been no study of the mechanism of brazilein in acoustic neuroma cells. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144800183039
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http://dx.doi.org/10.1016/j.yexmp.2018.12.010DOI Listing
April 2019
2 Reads

Analytical performance evaluation of the HPV OncoCheck assay for detection of high-risk HPV infection in liquid-based cervical samples.

Exp Mol Pathol 2019 02 31;106:149-156. Epub 2018 Dec 31.

Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Gangwon, South Korea.

Recent studies have demonstrated low specificity (false positive) of human papillomavirus (HPV) DNA testing for the screening and diagnosis of cervical samples. Therefore, we evaluated the performance of the HPV OncoCheck assay, which is an HPV E6/E7 mRNA-based assay, for the detection of 16 high-risk (HR)-HPVs including HPV 16 and HPV 18 genotypes in cervical samples using multiplex reverse transcriptase-quantitative PCR. In the present study, the analytical performance of the assay was evaluated using 16 HPV single strand DNAs. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.12.008DOI Listing
February 2019
2 Reads

HIVAN associated tubular pathology with reference to ER stress, mitochondrial changes, and autophagy.

Exp Mol Pathol 2019 02 31;106:139-148. Epub 2018 Dec 31.

Department of Neurology, University of Maryland, Baltimore, MD 21201, United States; VA Medical Center, Baltimore, MD 21201, United States. Electronic address:

Human immunodeficiency virus associated nephropathy (HIVAN) is a unique form of a renal parenchymal disorder. This disease and its characteristics can be accredited to incorporation of DNA and mRNA of human immunodeficiency virus type 1 into the renal parenchymal cells. A proper understanding of the intricacies of HIVAN and the underlying mechanisms associated with renal function and disorders is vital for the potential development of a reliable treatment for HIVAN. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.12.009DOI Listing
February 2019
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Lycium barbarum polysaccharides alleviate hydrogen peroxide-induced injury by up-regulation of miR-4295 in human trabecular meshwork cells.

Authors:
Yuxia Liu Yan Zhang

Exp Mol Pathol 2019 02 28;106:109-115. Epub 2018 Dec 28.

Department of Pathology, Huaihe Hospital of Henan University, Kaifeng, Henan 475000, China.

Glaucoma is a chronic neurodegenerative disease which produces damage to the optic nerve and causes sightlessness. Current remains lack of effective method for glaucoma. Lycium barbarum polysaccharides (LBPs) have pleiotropic effects on various diseases. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.12.007DOI Listing
February 2019
2 Reads

Suppression of Tescalcin inhibits growth and metastasis in renal cell carcinoma via downregulating NHE1 and NF-kB signaling.

Exp Mol Pathol 2019 04 27;107:110-117. Epub 2018 Dec 27.

The Third Xiangya Hospital of Central South University, Key Laboratory of Medical Information Research (Central South University), College of Hunan Province, Changsha 410013, PR China; Department of Urology, the Third Xiangya Hospital of Central South University, Changsha 410013, PR China. Electronic address:

Background: Renal cell carcinoma (RCC) is the most common form of kidney cancer. Recent studies reported that Tescalcin was overexpressed in various tumor types. However, the status of Tescalcin protein expression in RCC and its biological function is uncertain. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.12.004DOI Listing
April 2019
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Prognostic significance of circulating tumor cells (CTCs) in Egyptian non-metastatic colorectal cancer patients: A comparative study for four different techniques of detection (Flowcytometry, CellSearch, Quantitative Real-time PCR and Cytomorphology).

Exp Mol Pathol 2019 02 19;106:90-101. Epub 2018 Dec 19.

Medical Oncology Department, National Cancer Institute, Cairo University, Cairo 11976, Egypt.

Purpose: We assessed CTCs counts in NMCRC patients using four different techniques.

Methods: CTCs were detected in 63 NMCRC patients, 40 benign bowel diseases (BBD) and 40 normal controls (NC) using, flow-cytometry (FCM), CellSearch (CS), cytomorphology and quantitative real time (qPCR) for CK19, MUC1, CD44, CD133, ALDH1 expression. Results were correlated to progression free (PFS) and overall (OS). Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.12.006DOI Listing
February 2019
2 Reads

Ligustrazine promoted hypoxia-treated cell growth by upregulation of miR-135b in human umbilical vein endothelial cells.

Exp Mol Pathol 2019 02 18;106:102-108. Epub 2018 Dec 18.

Operating Room, Jining No.1 People's Hospital, Jining 272011, China; Affiliated Jining No.1 People's Hospital of Jining Medical University, Jining Medical University, Jining 272067, China. Electronic address:

Background: Pressure ulcers are a kind of troublesome disease which caused by long-term pressure and subsequently lead to tissue festering necrosis because of sustained ischemia, hypoxia and malnutrition. In our study, we used hypoxia to stimulate human umbilical vein endothelial cells (HUVECs) to mimic pressure ulcers and investigated the effects of Ligustrazine (Lig) with multi-activities on HUVECs.

Methods: HUVECs were treated by hypoxia to induce cell injury. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144800183041
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http://dx.doi.org/10.1016/j.yexmp.2018.12.005DOI Listing
February 2019
12 Reads

miR-122 enhances sensitivity of hepatocellular carcinoma to oxaliplatin via inhibiting MDR1 by targeting Wnt/β-catenin pathway.

Authors:
Fei Cao Li-Xue Yin

Exp Mol Pathol 2019 02 26;106:34-43. Epub 2018 Oct 26.

School of Medicine, University of Electronic Science and Technology of China, Chengdu 610054, P.R. China; Key Laboratory of Ultrasonic Cardiac Electrophysiology and Biomechanics of Sichuan Province, Department of Cardiovascular Ultrasound and Non-invasive Cardiology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu 610072, P.R. China. Electronic address:

Background: Hepatocellular carcinoma (HCC) is one of the primary causes of cancer-related death and resistance to cytotoxic chemotherapy is the major cause of mortality in HCC patients. miR-122 is a liver specific miRNA and is found to be reduced in HCC, however, the function of miR-122 in HCC chemosensitivity remains elusive.

Methods: We used qRT-PCR to measure expressions of miR-122, β-catenin and MDR1 in four HCC cell lines. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.10.009DOI Listing
February 2019
5 Reads

LncRNA-MEG3 alleviates high glucose induced inflammation and apoptosis of retina epithelial cells via regulating miR-34a/SIRT1 axis.

Exp Mol Pathol 2019 04 7;107:102-109. Epub 2018 Dec 7.

Department of Ophthalmology, The Third Xiangya Hospital of Central South University, Changsha 410013, PR China. Electronic address:

Background: Diabetic retinopathy (DR) is the serious complication of diabetes, which could lead to blindness. Inflammation and apoptosis are hallmark of DR, but mechanism of their regulation is little known. LncRNA-MEG3 is associated with multiple biological processes including proliferation, apoptosis and inflammation response, and is dramatically decreased in DR. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.12.003DOI Listing
April 2019
2 Reads

Singe nucleotide polymorphisms in osteosarcoma: Pathogenic effect and prognostic significance.

Exp Mol Pathol 2019 02 7;106:63-77. Epub 2018 Dec 7.

Thalassemia and Hemoglobinopathy Research Center, Research Institute of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Electronic address:

Purpose: Osteosarcoma (OS) is a common malignant bone tumor in children and adolescents. Pathogenesis and prognosis of OS can be associated with several environmental and genetic factors. Single nucleotide polymorphisms (SNPs) are crucial genetic changes that can be involved in clinical and therapeutic outcomes of OS. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144800183023
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http://dx.doi.org/10.1016/j.yexmp.2018.12.002DOI Listing
February 2019
17 Reads

Anti-inflammatory and antioxidant effect of melatonin on recovery from muscular trauma induced in rats.

Exp Mol Pathol 2019 02 4;106:52-59. Epub 2018 Dec 4.

Universidade Luterana do Brasil - ULBRA - Graduate Program in Genetics and Applied Toxicology, Canoas, Rio Grande do Sul, Brazil; Universidade Luterana do Brasil - ULBRA - Graduate Program in Cellular and Molecular Biology Applied to Health, Canoas, Rio Grande do Sul, Brazil; Universidade Federal do Rio Grande do Sul - UFRGS - Graduate Program in Medicine: Medical Sciences, Porto Alegre, Rio Grande do Sul, Brazil; Universidade Luterana do Brasil - Laboratory of Oxidative Stress and Antioxidants, Canoas, Rio Grande do Sul, Brazil; Hospital de Clínicas de Porto Alegre - HCPA - Laboratory of Experimental Hepatology and Gastroenterology, Porto Alegre, Rio Grande do Sul, Brazil. Electronic address:

In recent decades, the number of people who practice sports has grown exponentially, increasing the number of muscular injuries. Trauma injury occurs when the muscle is exposed to a sudden compression force. Melatonin (MLT) has often been cited in the literature as a potent antioxidant and anti-inflammatory agent. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144800183039
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http://dx.doi.org/10.1016/j.yexmp.2018.12.001DOI Listing
February 2019
16 Reads

Clonal analysis revealed functional heterogeneity in cancer stem-like cell phenotypes in uterine endometrioid adenocarcinoma.

Exp Mol Pathol 2019 02 30;106:78-88. Epub 2018 Nov 30.

Departments of Pathology, Sapporo Medical University School of Medicine, South-1 West-17, Chuo-Ku, Sapporo 060-8556, Japan; CREST, Japan Science and Technology Agency, Tokyo 102-0076, Japan. Electronic address:

Uterine endometrial carcinoma is one of the common cancers in females. Cancer stem-like cells (CSCs)/cancer-initiating cells (CICs) are a small subpopulation of cancer cells that are tumorigenic and are resistant to treatments, thus they are focused as treatment targets. However, the heterogeneity of CSCs/CICs is still elusive, and we therefore analyzed CSCs/CICs at the clonal level. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.11.013DOI Listing
February 2019
3 Reads