4,036 results match your criteria Experimental and Molecular Pathology [Journal]


p38 MAP kinases: plausible diagnostic and prognostic serum protein marker of non small cell lung cancer.

Exp Mol Pathol 2019 Feb 13. Epub 2019 Feb 13.

Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India. Electronic address:

Introduction: p38 MAPK signaling molecules plays a dual role in cancer, both progression and suppression. Elevated expression of p38α was reported in lung cancer tissue in rat model. Our objective was to explore the concentration of all 4 isoforms of p38MAPK in serum of Non Small Cell Lung Cancer (NSCLC). Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.01.009DOI Listing
February 2019

The clinical significance of endothelin receptor type B in hepatocellular carcinoma and its potential molecular mechanism.

Exp Mol Pathol 2019 Feb 12. Epub 2019 Feb 12.

Department of Pathology, First Affiliated Hospital of Guangxi Medical University, No. 6 Shuangyong Road, Nanning, Guangxi Zhuang Autonomous Region 530021, PR China. Electronic address:

Objective: To explore the clinical significance and potential molecular mechanism of endothelin receptor type B (EDNRB) in hepatocellular carcinoma (HCC).

Methods: Immunohistochemistry was used to detect EDNRB protein expression level in 67 HCC paraffin embedded tissues and adjacent tissues. Correlations between EDNRB expression level and clinicopathologic parameters were analyzed in our study. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.02.002DOI Listing
February 2019

miR-26b modulates OA induced BMSC osteogenesis through regulating GSK3β/β-catenin pathway.

Exp Mol Pathol 2019 Feb 12. Epub 2019 Feb 12.

Department of Orthopedics, Beijing Northern Hospital, China North Industries, Beijing 100089, PR.China.; Inner Mongolia Medical University, Hohhot 014010, Inner Mongolia, PR.China.. Electronic address:

Backgrounds: Osteoactivin (OA) is a key regulator promoting bone marrow stromal cells osteogenesis progress, while Dexamethasone (Dex) could inhibit OA induced osteogenesis and lead to osteoporosis. miR-26b increased during BMSC osteogenesis but whether it participates in this progress is enigma. Osteogenesis is under regulation of canonical Wnt signaling pathway which could serve as potential target for miR-26b. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.02.003DOI Listing
February 2019

Proteomic characterization of early lung response to breast cancer metastasis in mice.

Exp Mol Pathol 2019 Feb 11. Epub 2019 Feb 11.

Jagiellonian University, Jagiellonian Centre for Experimental Therapeutics (JCET), Bobrzynskiego 14, 30-348 Krakow, Poland; Jagiellonian University Medical College, Faculty of Pharmacy, Department of Toxicology, Medyczna 9, 30-688 Krakow, Poland. Electronic address:

Introduction: The tumor-promoting rearrangement of the lungs facilitates the process of cancer cell survival in a foreign microenvironment and enables their protection against immune defense. The study aimed to define the fingerprint of the early rearrangement of the lungs via the proteomic profiling of the lung tissue in the experimental model of tumor metastasis in a murine 4 T1 mammary adenocarcinoma.

Materials And Methods: The studies were performed on 7-8-week-old BALB/c female mice. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.02.001DOI Listing
February 2019

High levels of RAI3 expression is linked to shortened survival in esophageal cancer patients.

Exp Mol Pathol 2019 Jan 29;107:51-56. Epub 2019 Jan 29.

General, Visceral and Thoracic Surgery Department and Clinic, University Medical Center Hamburg-Eppendorf, Germany. Electronic address:

Expression of the retinoic acid-induced protein 3 (RAI3) has been suggested to predict clinical outcome in a variety of malignancies. However, its role in esophageal cancers remains unclear. Immunohistochemical RAI3 staining was analyzed on tissue microarrays containing 359 esophageal adenocarcinomas (EAC) and 254 esophageal squamous cell carcinomas (ESCC). Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.01.013DOI Listing
January 2019

LncRNA KCNQ1OT1 promoted BMP2 expression to regulate osteogenic differentiation by sponging miRNA-214.

Exp Mol Pathol 2019 Jan 28;107:77-84. Epub 2019 Jan 28.

Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha 410008, PR China. Electronic address:

Background: Osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is of much significance for bone formation, the imbalance of it would result in osteoporosis and other pathological bone defects. Increasing evidences showed that long non-coding RNAs (lncRNAs) and miRNAs played vital roles in the regulation of osteogenic differentiation. LncRNA KCNQ1OT1 was often regarded as an imprinted lncRNA and was related to tumor progression, while its function in osteogenic differentiation remained unclear. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144800183047
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http://dx.doi.org/10.1016/j.yexmp.2019.01.012DOI Listing
January 2019
3 Reads

Propofol suppresses proliferation and migration of papillary thyroid cancer cells by down-regulation of lncRNA ANRIL.

Exp Mol Pathol 2019 Jan 28;107:68-76. Epub 2019 Jan 28.

Department of Anesthesiology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China. Electronic address:

Background: Propofol is a popular anesthetic agent, with potent anti-tumor activity against many cancers. The objective of this study was to explore the potential effect of propofol on papillary thyroid cancer (PTC) in vitro.

Methods: Human PTC cell lines TPC-1 and IHH-4 were treated by propofol. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144800183032
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http://dx.doi.org/10.1016/j.yexmp.2019.01.011DOI Listing
January 2019
2 Reads

Silence of lncRNA CHRF protects H9c2 cells against lipopolysaccharide-induced injury via up-regulating microRNA-221.

Exp Mol Pathol 2019 Jan 26;107:43-50. Epub 2019 Jan 26.

Department of Pediatrics, Jining No.1 People's Hospital, Jining 272011, China. Electronic address:

Background: Cardiac hypertrophy related factor (CHRF), a newly recognized long non-coding RNA (lncRNA), is a central regulator in cardiac hypertrophy responses. This study attempted to show the potential role of lncRNA CHRF in bacterial infection caused myocarditis.

Methods: H9c2 cells were transfected with small interfering RNAs (siRNAs) specific for lncRNA CHRF alone or in combination with miR-221 inhibitor, and then subjected to lipopolysaccharide (LPS). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144800183042
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http://dx.doi.org/10.1016/j.yexmp.2019.01.010DOI Listing
January 2019
5 Reads
2.706 Impact Factor

Triptolide inhibits the growth and migration of colon carcinoma cells by down-regulation of miR-191.

Authors:
Yuxi Qi Jinliang Li

Exp Mol Pathol 2019 Jan 23;107:23-31. Epub 2019 Jan 23.

Department of Anorectal Surgery, Jining No.1 People's Hospital, Jining 272011, China. Electronic address:

Background: Triptolide (TPL) is the active component of Tripterygium wifordii Hook F, which has been reported to exert anti-tumor efficacies. Herein, we aimed to examine the efficacy of TPL in colorectal cancer (CRC) cells and to reveal its underlying mechanisms.

Methods: Human CRC cell lines HT-29 and SW480 were treated by TPL for 24 h in the presence or absence of epithelial-to-mesenchymal transition (EMT) inducer TGF-β1. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144800183048
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http://dx.doi.org/10.1016/j.yexmp.2019.01.008DOI Listing
January 2019
3 Reads

Silencing of the Na/H exchanger 1(NHE-1) prevents cardiac structural and functional remodeling induced by angiotensin II.

Exp Mol Pathol 2019 Jan 18;107:1-9. Epub 2019 Jan 18.

Centro de investigaciones Cardiovasculares "Dr. Horacio E, Cingolani", Facultad de Ciencias Médicas UNLP-CONICET, Argentina. Electronic address:

Chronic activation of the renin angiotensin system (RAS) favors several cardiac diseases, among which myocardial hypertrophy occupies an outstanding place. In this context, the hyperactivity of the cardiac Na/H (NHE-1) exchanger plays a key role. The pathologic remodeling of the myocardium constitutes an independent risk factor for morbidity and mortality with continuously increasing healthcare cost. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.01.007DOI Listing
January 2019
1 Read

The anti-malarial drug artesunate causes cell cycle arrest and apoptosis of triple-negative MDA-MB-468 and HER2-enriched SK-BR-3 breast cancer cells.

Exp Mol Pathol 2019 Jan 17;107:10-22. Epub 2019 Jan 17.

Department of Pathology, Dalhousie University, Halifax, Canada; Department of Microbiology and Immunology, Dalhousie University, Halifax, Canada; Department of Surgery, Dalhousie University, Halifax, Canada. Electronic address:

Breast cancer is the most prevalent cancer diagnosis in women, with triple-negative and human epidermal growth factor 2 (HER2)-enriched advanced breast cancers having the poorest prognoses. The morbidity and mortality associated with advanced disease, as well as the emergence of multi-drug resistant variants, highlights the urgency to develop novel therapeutic agents. Artesunate (ART) is a semi-synthetic derivative of artemisinin from the Chinese herb sweet wormwood. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.01.006DOI Listing
January 2019
2 Reads

Aluminum disrupts the prenatal development of the male and female gerbil prostate (Meriones unguiculatus).

Exp Mol Pathol 2019 Jan 17;107:32-42. Epub 2019 Jan 17.

Department of Histology, Embryology and Cell Biology, Laboratory of Microscopy Applied to Reproduction, Institute of Biological Sciences, Federal University of Goiás, Goiânia, Goiás 74001970, Brazil. Electronic address:

Normal prostate development is highly dependent of an equilibrated hormonal regulation, so that sensible interferences during this period may predispose the gland to lesions during aging. Industrial activities have increased the exposure of this gland to active elements found in environment, such as aluminum (Al). Al presents toxic effect for living beings, having the potential to disrupt the development and growth of several organs and systems. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.01.005DOI Listing
January 2019
1 Read

Effects of GST null genotypes on individual susceptibility to leukemia: A meta-analysis.

Exp Mol Pathol 2019 Jan 16. Epub 2019 Jan 16.

Department of Hematology, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215000, China; Institute of Blood and Marrow Transplantation, Collaborative Innovation Center of Hematology, Soochow University, Suzhou 215000, China; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Soochow University, Suzhou 215000, China. Electronic address:

Background: Whether glutathione S-transferases (GST) null genotypes influence individual susceptibility to leukemia remains controversial. Thus, we performed this meta-analysis to better analyze potential influences of GST null genotypes on individual susceptibility to leukemia.

Methods: Literature retrieve was conducted in PubMed, Web of Science and Embase. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.01.004DOI Listing
January 2019
2 Reads

Ginsenoside Rg3 inhibits cell growth, migration and invasion in Caco-2 cells by downregulation of lncRNA CCAT1.

Authors:
Jinliang Li Yuxi Qi

Exp Mol Pathol 2019 02 8;106:131-138. Epub 2019 Jan 8.

Department of Anorectal Surgery, Jining No.1 People's Hospital, Jining 272011, China. Electronic address:

Background: Colorectal cancer (CRC) is a troublesome disease with high morbidity and mortality. Ginsenoside Rg3 possesses anti-cancer properties. Colon Cancer Associated Transcript 1 (CCAT1) participates in the genesis, development, invasion and metastasis of colorectal cancer. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.01.003DOI Listing
February 2019

Baicalin protects human retinal pigment epithelial cell lines against high glucose-induced cell injury by up-regulation of microRNA-145.

Exp Mol Pathol 2019 02 6;106:123-130. Epub 2019 Jan 6.

Department of Ophthalmology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China. Electronic address:

Background: Diabetic retinopathy (DR) is a common complication of diabetes mellitus, which is a major reason of blindness. Baicalin (BAI) is a flavonoid extracted from Scutellaria baicalensis, whose pharmacological characterizes have been widely reported in various diseases. However, it remains unclear the effect of BAI on DR. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144800183034
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http://dx.doi.org/10.1016/j.yexmp.2019.01.002DOI Listing
February 2019
3 Reads

ZEB1 mediates doxorubicin (Dox) resistance and mesenchymal characteristics of hepatocarcinoma cells.

Exp Mol Pathol 2019 02 4;106:116-122. Epub 2019 Jan 4.

Interventional Vascular Surgery, Hunan Provincial People's Hospital, No. 61 Jiefangxi Road, Changsha 410005, China. Electronic address:

The acquired chemoresistance during long term chemotherapy is one of the most important factors to limit the application of Doxorubicin (Dox) on clinical treatment of hepatocellular carcinoma (HCC) patients. Our present study found that Dox resistant HCC (HCC/Dox) cells had greater capability of in vitro migration and invasion compared to their parental cells. HCC/Dox cells exhibited mesenchymal characteristics, which was evidenced by the up regulation of fibronectin, vimentin while down regulation of E-Cadherin. Read More

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http://dx.doi.org/10.1016/j.yexmp.2019.01.001DOI Listing
February 2019

Brazilein induces apoptosis and G1/G0 phase cell cycle arrest by up-regulation of miR-133a in human vestibular schwannoma cells.

Exp Mol Pathol 2019 Jan 2;107:95-101. Epub 2019 Jan 2.

Department of Chinese Medicine, Yantai Hospital of Traditional Chinese Medicine, Yantai 264000, China. Electronic address:

Background: Acoustic neuroma is a benign and usually slow growing tumor. Brazilein is a natural compound extracted from hematoxylin. However, there has been no study of the mechanism of brazilein in acoustic neuroma cells. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144800183039
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http://dx.doi.org/10.1016/j.yexmp.2018.12.010DOI Listing
January 2019
1 Read

Analytical performance evaluation of the HPV OncoCheck assay for detection of high-risk HPV infection in liquid-based cervical samples.

Exp Mol Pathol 2019 02 31;106:149-156. Epub 2018 Dec 31.

Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Gangwon, South Korea.

Recent studies have demonstrated low specificity (false positive) of human papillomavirus (HPV) DNA testing for the screening and diagnosis of cervical samples. Therefore, we evaluated the performance of the HPV OncoCheck assay, which is an HPV E6/E7 mRNA-based assay, for the detection of 16 high-risk (HR)-HPVs including HPV 16 and HPV 18 genotypes in cervical samples using multiplex reverse transcriptase-quantitative PCR. In the present study, the analytical performance of the assay was evaluated using 16 HPV single strand DNAs. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.12.008DOI Listing
February 2019

HIVAN associated tubular pathology with reference to ER stress, mitochondrial changes, and autophagy.

Exp Mol Pathol 2019 02 31;106:139-148. Epub 2018 Dec 31.

Department of Neurology, University of Maryland, Baltimore, MD 21201, United States; VA Medical Center, Baltimore, MD 21201, United States. Electronic address:

Human immunodeficiency virus associated nephropathy (HIVAN) is a unique form of a renal parenchymal disorder. This disease and its characteristics can be accredited to incorporation of DNA and mRNA of human immunodeficiency virus type 1 into the renal parenchymal cells. A proper understanding of the intricacies of HIVAN and the underlying mechanisms associated with renal function and disorders is vital for the potential development of a reliable treatment for HIVAN. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.12.009DOI Listing
February 2019
4 Reads

Lycium barbarum polysaccharides alleviate hydrogen peroxide-induced injury by up-regulation of miR-4295 in human trabecular meshwork cells.

Authors:
Yuxia Liu Yan Zhang

Exp Mol Pathol 2019 02 28;106:109-115. Epub 2018 Dec 28.

Department of Pathology, Huaihe Hospital of Henan University, Kaifeng, Henan 475000, China.

Glaucoma is a chronic neurodegenerative disease which produces damage to the optic nerve and causes sightlessness. Current remains lack of effective method for glaucoma. Lycium barbarum polysaccharides (LBPs) have pleiotropic effects on various diseases. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.12.007DOI Listing
February 2019

Suppression of Tescalcin inhibits growth and metastasis in renal cell carcinoma via downregulating NHE1 and NF-kB signaling.

Exp Mol Pathol 2018 Dec 27. Epub 2018 Dec 27.

The Third Xiangya Hospital of Central South University, Key Laboratory of Medical Information Research (Central South University), College of Hunan Province, Changsha 410013, PR China; Department of Urology, the Third Xiangya Hospital of Central South University, Changsha 410013, PR China. Electronic address:

Background: Renal cell carcinoma (RCC) is the most common form of kidney cancer. Recent studies reported that Tescalcin was overexpressed in various tumor types. However, the status of Tescalcin protein expression in RCC and its biological function is uncertain. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.12.004DOI Listing
December 2018
1 Read

Prognostic significance of circulating tumor cells (CTCs) in Egyptian non-metastatic colorectal cancer patients: A comparative study for four different techniques of detection (Flowcytometry, CellSearch, Quantitative Real-time PCR and Cytomorphology).

Exp Mol Pathol 2019 02 19;106:90-101. Epub 2018 Dec 19.

Medical Oncology Department, National Cancer Institute, Cairo University, Cairo 11976, Egypt.

Purpose: We assessed CTCs counts in NMCRC patients using four different techniques.

Methods: CTCs were detected in 63 NMCRC patients, 40 benign bowel diseases (BBD) and 40 normal controls (NC) using, flow-cytometry (FCM), CellSearch (CS), cytomorphology and quantitative real time (qPCR) for CK19, MUC1, CD44, CD133, ALDH1 expression. Results were correlated to progression free (PFS) and overall (OS). Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.12.006DOI Listing
February 2019

Ligustrazine promoted hypoxia-treated cell growth by upregulation of miR-135b in human umbilical vein endothelial cells.

Exp Mol Pathol 2019 02 18;106:102-108. Epub 2018 Dec 18.

Operating Room, Jining No.1 People's Hospital, Jining 272011, China; Affiliated Jining No.1 People's Hospital of Jining Medical University, Jining Medical University, Jining 272067, China. Electronic address:

Background: Pressure ulcers are a kind of troublesome disease which caused by long-term pressure and subsequently lead to tissue festering necrosis because of sustained ischemia, hypoxia and malnutrition. In our study, we used hypoxia to stimulate human umbilical vein endothelial cells (HUVECs) to mimic pressure ulcers and investigated the effects of Ligustrazine (Lig) with multi-activities on HUVECs.

Methods: HUVECs were treated by hypoxia to induce cell injury. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144800183041
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http://dx.doi.org/10.1016/j.yexmp.2018.12.005DOI Listing
February 2019
6 Reads

miR-122 enhances sensitivity of hepatocellular carcinoma to oxaliplatin via inhibiting MDR1 by targeting Wnt/β-catenin pathway.

Authors:
Fei Cao Li-Xue Yin

Exp Mol Pathol 2019 02 26;106:34-43. Epub 2018 Oct 26.

School of Medicine, University of Electronic Science and Technology of China, Chengdu 610054, P.R. China; Key Laboratory of Ultrasonic Cardiac Electrophysiology and Biomechanics of Sichuan Province, Department of Cardiovascular Ultrasound and Non-invasive Cardiology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu 610072, P.R. China. Electronic address:

Background: Hepatocellular carcinoma (HCC) is one of the primary causes of cancer-related death and resistance to cytotoxic chemotherapy is the major cause of mortality in HCC patients. miR-122 is a liver specific miRNA and is found to be reduced in HCC, however, the function of miR-122 in HCC chemosensitivity remains elusive.

Methods: We used qRT-PCR to measure expressions of miR-122, β-catenin and MDR1 in four HCC cell lines. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.10.009DOI Listing
February 2019

LncRNA-MEG3 alleviates high glucose induced inflammation and apoptosis of retina epithelial cells via regulating miR-34a/SIRT1 axis.

Exp Mol Pathol 2018 Dec 7. Epub 2018 Dec 7.

Department of Ophthalmology, The Third Xiangya Hospital of Central South University, Changsha 410013, PR China. Electronic address:

Background: Diabetic retinopathy (DR) is the serious complication of diabetes, which could lead to blindness. Inflammation and apoptosis are hallmark of DR, but mechanism of their regulation is little known. LncRNA-MEG3 is associated with multiple biological processes including proliferation, apoptosis and inflammation response, and is dramatically decreased in DR. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.12.003DOI Listing
December 2018

Singe nucleotide polymorphisms in osteosarcoma: Pathogenic effect and prognostic significance.

Exp Mol Pathol 2019 02 7;106:63-77. Epub 2018 Dec 7.

Thalassemia and Hemoglobinopathy Research Center, Research Institute of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Electronic address:

Purpose: Osteosarcoma (OS) is a common malignant bone tumor in children and adolescents. Pathogenesis and prognosis of OS can be associated with several environmental and genetic factors. Single nucleotide polymorphisms (SNPs) are crucial genetic changes that can be involved in clinical and therapeutic outcomes of OS. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144800183023
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http://dx.doi.org/10.1016/j.yexmp.2018.12.002DOI Listing
February 2019
8 Reads

Anti-inflammatory and antioxidant effect of melatonin on recovery from muscular trauma induced in rats.

Exp Mol Pathol 2019 02 4;106:52-59. Epub 2018 Dec 4.

Universidade Luterana do Brasil - ULBRA - Graduate Program in Genetics and Applied Toxicology, Canoas, Rio Grande do Sul, Brazil; Universidade Luterana do Brasil - ULBRA - Graduate Program in Cellular and Molecular Biology Applied to Health, Canoas, Rio Grande do Sul, Brazil; Universidade Federal do Rio Grande do Sul - UFRGS - Graduate Program in Medicine: Medical Sciences, Porto Alegre, Rio Grande do Sul, Brazil; Universidade Luterana do Brasil - Laboratory of Oxidative Stress and Antioxidants, Canoas, Rio Grande do Sul, Brazil; Hospital de Clínicas de Porto Alegre - HCPA - Laboratory of Experimental Hepatology and Gastroenterology, Porto Alegre, Rio Grande do Sul, Brazil. Electronic address:

In recent decades, the number of people who practice sports has grown exponentially, increasing the number of muscular injuries. Trauma injury occurs when the muscle is exposed to a sudden compression force. Melatonin (MLT) has often been cited in the literature as a potent antioxidant and anti-inflammatory agent. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144800183039
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http://dx.doi.org/10.1016/j.yexmp.2018.12.001DOI Listing
February 2019
10 Reads

Clonal analysis revealed functional heterogeneity in cancer stem-like cell phenotypes in uterine endometrioid adenocarcinoma.

Exp Mol Pathol 2019 02 30;106:78-88. Epub 2018 Nov 30.

Departments of Pathology, Sapporo Medical University School of Medicine, South-1 West-17, Chuo-Ku, Sapporo 060-8556, Japan; CREST, Japan Science and Technology Agency, Tokyo 102-0076, Japan. Electronic address:

Uterine endometrial carcinoma is one of the common cancers in females. Cancer stem-like cells (CSCs)/cancer-initiating cells (CICs) are a small subpopulation of cancer cells that are tumorigenic and are resistant to treatments, thus they are focused as treatment targets. However, the heterogeneity of CSCs/CICs is still elusive, and we therefore analyzed CSCs/CICs at the clonal level. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.11.013DOI Listing
February 2019

An unexpected diagnosis of histiocytic sarcoma.

Exp Mol Pathol 2019 02 28;106:60-62. Epub 2018 Nov 28.

Harbor-UCLA Medical Center, 1000 W. Carson St., Torrance, CA 90509, United States. Electronic address:

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http://dx.doi.org/10.1016/j.yexmp.2018.11.012DOI Listing
February 2019

MiR-141-3p suppresses gastric cancer induced transition of normal fibroblast and BMSC to cancer-associated fibroblasts via targeting STAT4.

Exp Mol Pathol 2018 Nov 28;107:85-94. Epub 2018 Nov 28.

Department of Gastrointestinal Surgery, Fujian Cancer Hospital & Fujian Medical University Cancer Hospital, Fuzhou 350001, Fujian, China. Electronic address:

Background: Cancer associated fibroblasts (CAFs) are known to be crucial constituents of cancer microenvironment (CME) and play an important role in initiation, progression and metastasis of various types of cancer, such as oral cancer, pancreatic cancer, and gastric cancer. CAFs are usually derived from normal fibroblasts (NFs), but the mechanism of the transition in gastric cancer has not yet been fully elucidated.

Methods: qRT-PCR and western blot were employed to investigate differences of miR-141 and STAT4 expression respectively. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.11.014DOI Listing
November 2018

Abnormal response of costal chondrocytes to acidosis in patients with chest wall deformity.

Exp Mol Pathol 2019 02 25;106:27-33. Epub 2018 Nov 25.

Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA. Electronic address:

Costal cartilage is much understudied compared to the load bearing cartilages. Abnormally grown costal cartilages are associated with the inherited chest wall deformities pectus excavatum and pectus carinatum resulting in sunken or pigeon chest respectively. A lack of understanding of the ultrastructural and molecular biology properties of costal cartilage is a major confounder in predicting causes and outcomes of these disorders. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.11.011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368461PMC
February 2019
1 Read

Long non-coding RNAs and cervical cancer.

Exp Mol Pathol 2019 02 22;106:7-16. Epub 2018 Nov 22.

Department of Molecular Genetics, Ahar Branch, Islamic Azad University, Ahar, Iran. Electronic address:

Cervical cancer is determined as the second highest number of deaths factor in female cancers. Here is a need to find new biomarkers for detection and preliminary prognosis, metastasis. To find new treatment to enhance the survival of cervical cancer patients, pivotal actions are necessitated to be implemented. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.11.010DOI Listing
February 2019
77 Reads
2.706 Impact Factor

Molecular and cellular genetics of non-Hodgkin lymphoma: Diagnostic and prognostic implications.

Exp Mol Pathol 2019 02 19;106:44-51. Epub 2018 Nov 19.

University of Oklahoma Health Sciences Center (OUHSC), Department of Pathology, 940 Stanton L. Young Blvd, BMSB 451, Oklahoma City, OK 73104, USA. Electronic address:

Non-Hodgkin lymphoma (NHL) is a diverse collection of malignant neoplasms with lymphoid-cell origin which includes all the malignant lymphomas that are not classified as Hodgkin lymphoma. NHL is one of the most common types of cancer diagnosed in men and women in the developed world. In the United States of America, the past few decades have seen a significant rise in the incidence of NHL and it accounts for about 4% of all cancers now. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.11.008DOI Listing
February 2019
21 Reads

Knockdown on aPKC-ι inhibits epithelial-mesenchymal transition, migration and invasion of colorectal cancer cells through Rac1-JNK pathway.

Exp Mol Pathol 2018 Nov 19;107:57-67. Epub 2018 Nov 19.

Department of General Surgery, Xinqiao Hospital, Army Medical University, Chongqing 400037, PR China. Electronic address:

Background: Atypical protein kinase C-ι (aPKC-ι) is an oncogenic factor, and required for the epithelial-mesenchymal transition (EMT) of different types of cancer. Our study aimed to investigate the role of aPKC-ι in the EMT, migration and invasion of colorectal cancer (CRC) cells.

Methods: Expression of aPKC-ι was evaluated in CRC cell lines treated with TGF-β1 using qPCR and western blot. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.11.007DOI Listing
November 2018
13 Reads

TKS5-positive invadopodia-like structures in human tumor surgical specimens.

Exp Mol Pathol 2019 02 12;106:17-26. Epub 2018 Nov 12.

Division on Medical Oncology Hematology, Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, CA, USA; David Geffen School of Medicine at UCLA, Los Angeles, CA, USA; Jonsson Comprehensive Cancer Center, UCLA, Los Angeles, CA, USA. Electronic address:

Invadopodia, cancer cell protrusions with proteolytic activity, are functionally associated with active remodeling of the extracellular matrix. Here, we show that the invadopodia-related protein TKS5 is expressed in human pancreatic adenocarcinoma lines, and demonstrate that pancreatic cancer cells depend on TKS5 for invadopodia formation and function. Immunofluorescence staining of human pancreatic cancer cells reveals that TKS5 is a marker of mature and immature invadopodia. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368464PMC
February 2019
7 Reads

Genome-wide DNA methylation assessment of 'BRCA1-like' early-onset breast cancer: Data from the Australian Breast Cancer Family Registry.

Exp Mol Pathol 2018 12 10;105(3):404-410. Epub 2018 Nov 10.

Genetic Epidemiology Laboratory, Department of Clinical Pathology, The University of Melbourne, VIC 3010, Australia; Precision Medicine, School of Clinical Sciences at Monash Health, Monash University, Clayton 3168, Australia; Cancer Epidemiology and Intelligence Division, Cancer Council Victoria, VIC 3004, Australia. Electronic address:

Breast cancers arising in women carrying a germline mutation in BRCA1 are typically high-grade, early-onset and have distinct morphological features (BRCA1-like). However, the majority of early-onset breast cancers of this morphological type are not associated with germline BRCA1 mutations or constitutional BRCA1 promoter methylation. We aimed to assess DNA methylation across the genome for associations with the "BRCA1-like" morphology. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.11.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6289279PMC
December 2018
6 Reads
2.710 Impact Factor

Molecular biomarkers of Graves' ophthalmopathy.

Exp Mol Pathol 2019 02 8;106:1-6. Epub 2018 Nov 8.

Department of Regenerative & Cancer Cell Biology, Albany Medical College, Albany, New York 12208, United States. Electronic address:

Graves' ophthalmopathy (GO), a complication of Graves' disease (GD), is typified by orbital inflammation, ocular tissue expansion and remodeling and, ultimately, fibrosis. Orbital fibroblasts are key effectors of GO pathogenesis exhibiting exaggerated inflammatory and fibroproliferative responses to cytokines released by infiltrating immune cells. Activated orbital fibroblasts also produce inflammatory mediators that contribute to disease progression, facilitate the orbital trafficking of monocytes and macrophages, promote differentiation of matrix-producing myofibroblasts and stimulate accumulation of a hyaluronan-rich stroma, which leads to orbital tissue edema and fibrosis. Read More

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February 2019
13 Reads

Molecular evaluation of BRAF gene mutation in thyroid tumors: Significant association with papillary tumors and extra thyroidal extension indicating its role as a biomarker of aggressive disease.

Exp Mol Pathol 2018 12 8;105(3):380-386. Epub 2018 Nov 8.

Research and Development Division, SRL Ltd, Plot No.1, Prime Square Building, S.V.Road, Goregaon (W), Mumbai, India. Electronic address:

Background: Somatic mutation of the BRAF gene is one to be the most commonly known genetic change in thyroid tumors especially papillary thyroid cancers. The T1799A activating point mutation is detected in >98% of the thyroid tumors, and result in substitution of amino acid valine at position 600 to glutamic acid.

Method: In this study, we evaluated BRAF mutation in 95 Indian thyroid tumors by pyrosequencing assay. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.11.002DOI Listing
December 2018
9 Reads

MicroRNA-sequencing data analyzing melanoma development and progression.

Exp Mol Pathol 2018 12 8;105(3):371-379. Epub 2018 Nov 8.

Institute of Biochemistry, Emil-Fischer-Center, Friedrich-Alexander-University Erlangen-Nürnberg, 91054 Erlangen, Germany. Electronic address:

MicroRNAs (miRNAs) deregulated in melanoma are of growing importance in cancer research. We aimed to define the miRNAome of melanoma cell lines and primary melanocytes by RNA-Seq using identical cell lines as in a published miRNA expression study based on cDNA arrays. We identified 79 miRNAs, which are significantly deregulated during melanoma development. Read More

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December 2018
10 Reads

Smooth muscle cell differentiation from rabbit amniotic cells.

Exp Mol Pathol 2018 12 8;105(3):395-403. Epub 2018 Nov 8.

Department of Medical Genetics, Yeditepe University Medical School, 34755 Istanbul, Turkey. Electronic address:

Amniotic fluid (AF) is the liquid layer that provides mechanical support and allows movement of the fetus during embryogenesis. Mesenchymal stem cells (MSCs), which have differentiation capacity, are also found in AF-derived cells at a low ratio. Smooth muscle cells (SMCs) play an important role in organ function and are frequently used in tissue engineering. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.11.003DOI Listing
December 2018
7 Reads

A-kinase anchor protein 12 (AKAP12) inhibits cell migration in breast cancer.

Exp Mol Pathol 2018 12 30;105(3):364-370. Epub 2018 Oct 30.

Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, 4 Medical Drive, MD10, 117594 Singapore, Singapore. Electronic address:

A-kinase anchor protein 12 (AKAP12) also known as Gravin and SSeCKS, is a novel potent scaffold protein for many key signaling factors, such as protein kinase C (PKC), PKA, cyclins as well as F-actin. AKAP12 expression is known to be suppressed in several human malignancies including breast, prostate, gastric and colon cancers. In this study, we evaluated the role of AKAP12 in the migration of breast cancer cells, an important cellular process in cancer progression. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.10.010DOI Listing
December 2018
10 Reads

Corrigendum to "AQP4 and HIVAN" Experimental and Molecular Pathology, 105(2018) 71-75.

Exp Mol Pathol 2018 12 22;105(3):417. Epub 2018 Oct 22.

Department of Neurology, University of Maryland, Baltimore, MD, United States; VA Medical Center, Baltimore, MD, United States. Electronic address:

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December 2018

Hybrid oncocytic/chromophobe renal cell tumor: An integrated genetic and epigenetic characterization of a case.

Exp Mol Pathol 2018 12 18;105(3):352-356. Epub 2018 Oct 18.

Department of Pathology, Portuguese Oncology Institute of Porto, Portugal; Cancer Biology & Epigenetics Group, Research Center, Portuguese Oncology Institute of Porto, Portugal; Department of Pathology and Molecular Immunology, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Portugal. Electronic address:

Introduction: Hybrid oncocytic/chromophobe tumor (HOCT) is a renal cell neoplasm displaying overlapping cellular and architectural features of both renal oncocytoma (RO) and chromophobe renal cell carcinoma (chRCC). It has been described in the context of oncocytosis, Birt-Hogg-Dubé syndrome, and also sporadically. Thus far, HOCT immunohischemical profile and cytogenetic alterations have been reported, but not epigenetic alterations. Read More

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December 2018
6 Reads

Analysis of point mutations and copy number variation in Grade II and III meningioma.

Exp Mol Pathol 2018 12 11;105(3):328-333. Epub 2018 Oct 11.

Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA. Electronic address:

Meningiomas are among the most common tumors of the adult central nervous system (CNS). They are classified by the World Health Organization into three pathologic grades with increasing severity: grade I are benign with favorable treatment outcomes and low recurrence rates while grade III display malignant behavior and poor progression-free survival. Previous studies have shown that inactivation of NF-2 is the most common genetic event in high-grade meningioma; however, there is dearth of molecular data to distinguish grade II (AM-II) from the even more aggressive grade III (AM-III). Read More

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December 2018
11 Reads

Basidiomycetes-X, an edible mushroom, alleviates the development of atopic dermatitis in NC/Nga mouse model.

Exp Mol Pathol 2018 12 9;105(3):322-327. Epub 2018 Oct 9.

Department of Clinical Pharmacology, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences, 265-1, Higashijima, Akiha ku, Niigata 956-8603, Japan; National Institute of Pharmaceutical Education and Research, Chuilal Bhawan, 168 Manicktala Main Road, Kolkata 700054, West Bengal, India. Electronic address:

Basidiomycetes-X (BDM-X) is a novel edible mushroom recently identified as a new fungi species and is effective against oxidative stress and anti-inflammation associated with immune response. However the effect of BDM-X on atopic dermatitis (AD) has not been elucidated. In this study, we have investigated the effect of BDM-X on AD skin lesions in NC/Nga mouse model. Read More

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December 2018
9 Reads

MiR-10 targets NgR to modulate the proliferation of microglial cells and the secretion of inflammatory cytokines.

Exp Mol Pathol 2018 12 9;105(3):357-363. Epub 2018 Oct 9.

Department of Orthopedics, The Third Xiangya Hospital of Central South University, China.. Electronic address:

The present study is aimed to investigate the molecular mechanism of miR-10 targeting NgR to regulate the proliferation and inflammatory cytokine secretion in microglia cells (MCs). The expression levels of NgR, IL-1β, and TNF-α in rat MCs were detected by QPCR and Immunoblotting assays. The proliferation of MC was detected by MTT and Soft agar clone formation assays. Read More

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December 2018
1 Read

Cardioprotection of benzolamide in a regional ischemia model: Role of eNOS/NO.

Exp Mol Pathol 2018 12 9;105(3):345-351. Epub 2018 Oct 9.

"Dr Horacio E. Cingolani", Universidad Nacional de La Plata, La Plata, Argentina. Electronic address:

Background: Recent studies from our laboratory show the cardioprotective action of benzolamide (BZ, carbonic anhydrase inhibitor) against ischemia-reperfusion injury. However, the mechanisms involved have not been fully elucidated.

Objective: To examine the participation of the endothelial nitric oxide synthase (eNOS)/nitric oxide (NO) in the effects of BZ in a model of regional ischemia. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.10.003DOI Listing
December 2018
11 Reads

Insights into hepatic and renal FXR/DDAH-1/eNOS pathway and its role in the potential benefit of rosuvastatin and silymarin in hepatic nephropathy.

Exp Mol Pathol 2018 12 9;105(3):293-310. Epub 2018 Oct 9.

Department of Histology and Cell Biology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.

Objectives: The repression of renal Farnesoid X Receptor (FXR) had been shown to result from lack of bile acid production from cirrhotic liver. We hypothesized that silymarin and rosuvastatin (Rvs) could have a hepatorenal therapeutic effects in hepatic nephropathy through induction of FXR.

Methods: Forty two male Wistar rats were used; naïve (n = 12); six of them were sacrificed after 4 weeks and six continued till the end of the experiment. Read More

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December 2018
7 Reads

Upregulation of miR-132 contributes to the pathophysiology of COPD via targeting SOCS5.

Exp Mol Pathol 2018 12 4;105(3):285-292. Epub 2018 Oct 4.

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Xi'an Medical University, Xi'an City, Shaanxi Province, China.

The role of microRNAs has been recently identified in chronic obstructive pulmonary disease (COPD). This study aimed to examine the role of miR-132 in the pathophysiology of COPD and to explore the underlying molecular mechanisms of miR-132 in COPD. MiR-132 and suppressor of cytokine signaling 5 (SOCS5) mRNA expression were detected by qRT-PCR. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.10.002DOI Listing
December 2018
10 Reads

Association of STAT4, TGFβ1, SH2B3 and PTPN22 polymorphisms with autoimmune hepatitis.

Exp Mol Pathol 2018 12 3;105(3):279-284. Epub 2018 Oct 3.

Department of Immunology, Military Hospital of Tunis, Montfleury 1008, Tunis, Tunisia.

The physiopathology of autoimmune hepatitis (AIH) is complex and still not fully elucidated. The genes localized outside the histocompatibility complex involved in regulation and signal transduction of the immune system SH2B3, TGFβ1, STAT4 and PTPN22 could be associated to the susceptibility and hepatocyte lysis mechanism of this lethal autoimmune disorder.

Patients And Methods: We investigated four polymorphic sites in SH2B3 (rs3184504), TGFβ1 (rs1800471), STAT4 (rs7574865) and PTPN22 (rs2476601) in 45 AIH patients and 150 healthy controls from Tunisia using real-time PCR. Read More

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http://dx.doi.org/10.1016/j.yexmp.2018.10.001DOI Listing
December 2018
1 Read