1,884 results match your criteria Experimental Lung Research[Journal]


Opposite alterations of endothelin-1 in lung and pulmonary artery mirror gene expression of bone morphogenetic protein receptor 2 in experimental pulmonary hypertension.

Exp Lung Res 2019 Apr 23:1-12. Epub 2019 Apr 23.

a Department of Pharmacology and Toxicology, Faculty of Pharmacy , Comenius University in Bratislava , Bratislava , Slovakia.

Aim Of The Study: Endothelin-1 (ET-1) overexpression was suggested to play a role in pulmonary hypertension (PH). However, the roles of ET-1 in early stages of PH remain unexplored. We examined the expression of ET-1 and relevant disease progression markers in the pulmonary artery and the lungs during the development of PH induced by monocrotaline (MCT). Read More

View Article

Download full-text PDF

Source
https://www.tandfonline.com/doi/full/10.1080/01902148.2019.1
Publisher Site
http://dx.doi.org/10.1080/01902148.2019.1605426DOI Listing
April 2019
1 Read

A comparative in vitro study of standard facemask jet nebulization and high-flow nebulization in bronchiolitis.

Exp Lung Res 2019 Apr 22:1-9. Epub 2019 Apr 22.

c Chemical Engineering Division, Department of Biotechnology and Food Science , Universidad de Burgos , Burgos , Spain.

Aim Of Study: The use of a nebulizer paired with high-flow nasal cannulas (HFNC) has been proposed for drug delivery in bronchiolitis. Particle size nebulized is a relevant factor determining the efficacy of the nebulization. We replicated in vitro the theoretical parameters most widely used in bronchiolitis and we compared the size of the droplet nebulized with a standard nebulizer and a nebulizer integrated into HFNC. Read More

View Article

Download full-text PDF

Source
https://www.tandfonline.com/doi/full/10.1080/01902148.2019.1
Publisher Site
http://dx.doi.org/10.1080/01902148.2019.1599084DOI Listing
April 2019
1 Read

The Hedgehog target Gli1 is not required for bleomycin-induced lung fibrosis.

Exp Lung Res 2019 Apr 13:1-8. Epub 2019 Apr 13.

a Division of Pulmonary, Critical Care and Sleep Medicine , New York School of Medicine and Langone Medical Center , New York , NY , USA.

Sonic Hedgehog (SHH) signaling, a developmental pathway promoting lung mesenchymal expansion and differentiation during embryogenesis, has been increasingly recognized as a profibrotic factor in mature lung, where it might contribute to the pathogenesis of lung fibrosis. Pathway inhibition at the level of the downstream Gli transcription factors Gli1 and Gli2 (by GANT61) ameliorates lung fibrosis in the bleomycin model, whereas inhibition proximally at the level of HH ligand (by anti Hh antibody 5E1) or Smo (by GDC-0449) of the canonical pathway does not, implicating Gli1 and/or Gli2 as a key target. The fact that both the Gli1-labelled cell lineage and Gli1 expressing cells expand during fibrosis formation and contribute significantly to the pool of myofibroblasts in the fibrosis scars suggests a fibrogenic role for Gli1. Read More

View Article

Download full-text PDF

Source
https://www.tandfonline.com/doi/full/10.1080/01902148.2019.1
Publisher Site
http://dx.doi.org/10.1080/01902148.2019.1601795DOI Listing
April 2019
2 Reads

Quantitative proteomics reveals the mechanisms of hydrogen-conferred protection against hyperoxia-induced injury in type II alveolar epithelial cells.

Exp Lung Res 2019 Apr 11:1-12. Epub 2019 Apr 11.

a Department of Pediatric Intensive Care Unit , Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders , Chongqing , China.

Purpose/Aim: Exposure to hyperoxia leads to lung injury both in vivo and in vitro, molecular hydrogen has been reported to protect against hyperoxia-induced lung injury; however, the underlying molecular mechanisms remain largely unknown. The objective of this study was to characterize differentially regulated proteins and biological processes in hydrogen-treated hyperoxic primary type II alveolar epithelial cells (AECIIs) to elucidate the protective mechanism of hydrogen using quantitative proteomics. Materials and Methods: AECIIs were divided into three groups that were cultured for 24 h in three different conditions: control (21% oxygen), hyperoxia (95% oxygen), and hyperoxia + hydrogen. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2019.1601296DOI Listing
April 2019
1.754 Impact Factor

Roles of citric acid in conjunction with saline nebulization in experimental tracheostomy in guinea pigs.

Exp Lung Res 2019 Apr 11:1-10. Epub 2019 Apr 11.

c Department of Neurosurgery , the First Affiliated Hospital of Soochow University , Suzhou , China.

Purpose: Tracheostomy usually accompanied by the impairment of cough reflex, which may affect the clearance of secretions and result in the occurrence and development of pulmonary inflammation. Previous research has demonstrated that citric acid could effectively evoke cough. However, there are limited data available on this topic specific to the cough stimulation method, and the roles of citric acid in tracheostomy still remain obscure. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1516832DOI Listing
April 2019
1 Read

Role of prostaglandin I in the bronchoconstriction-triggered cough response in guinea pigs.

Exp Lung Res 2019 Apr 1:1-9. Epub 2019 Apr 1.

d Hematology Medicine , Kanazawa University Hospital , Ishikawa , Japan.

Purpose/Aim of the study: Methacholine chloride (MCh) inhalation causes bronchoconstriction and cough. Following MCh-induced bronchoconstriction, metabolic products of prostaglandin I (PGI) increase in bronchoalveolar lavage fluid (BALF), suggesting that PGI plays a role in the cough response. Accordingly, we used an experimental guinea pig model to evaluate the role of PGI in the bronchoconstriction-triggered cough response. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2019.1590883DOI Listing
April 2019
1 Read

Biphasic changes in airway epithelial cell EGF receptor binding and phosphorylation induced by components of hogbarn dust.

Exp Lung Res 2019 Mar 12:1-12. Epub 2019 Mar 12.

a Department of Pharmacology and Experimental Neuroscience , University of Nebraska Medical Center , Omaha , NE , USA.

Purpose Of The Study: Workers in enclosed hogbarns experience an increased incidence of airway inflammation and obstructive lung disease, and an aqueous hogbarn dust extract (HDE) induces multiple inflammation-related responses in cultured airway epithelial cells. Epidermal growth factor receptor (EGFR) phosphorylation and activation has been identified as one important mediator of inflammatory cytokine release from these cells. The studies here investigated both early and late phase adaptive changes in EGFR binding properties and subcellular localization induced by exposure of cells to HDE. Read More

View Article

Download full-text PDF

Source
https://www.tandfonline.com/doi/full/10.1080/01902148.2019.1
Publisher Site
http://dx.doi.org/10.1080/01902148.2019.1575931DOI Listing
March 2019
3 Reads

Pulmonary toxicity following inhalation exposure to VX in anesthetized rats: Possible roles for compromised immunity and oxidative stress-induced lung injury.

Exp Lung Res 2018 Oct - Nov;44(8-9):379-396. Epub 2019 Feb 22.

a Biochemical and Physiology Branch, Medical Toxicology Research Division , United States Army Medical Research Institute of Chemical Defense , Aberdeen Proving Ground , Maryland , USA.

The nerve agent VX is one of the most deadly threat agents available in weapons stockpiles for intentional release. While mostly considered a percutaneous toxicant, it can be fatal when aerosolized. The objective of this study was to investigate toxic responses in the lung up to two weeks following a single 10-minute exposure to inhaled VX. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1519003DOI Listing
February 2019
3 Reads

Hyperoxia-induced lung injury increases CDKN1A levels in a newborn rat model of bronchopulmonary dysplasia.

Exp Lung Res 2018 Oct - Nov;44(8-9):424-432. Epub 2019 Feb 12.

a Department of Pediatrics , Shengjing Hospital affiliated to China Medical University , Shenyang , P.R. China.

Bronchopulmonary dysplasia (BPD) is a common, chronic lung disease of infants. Presently, high oxygen exposure and mechanical ventilation considerably influence the development of BPD. To clarify the pathological mechanisms of this disease, we developed a hyperoxia-induced BPD rat model and investigated the role of CDKN1A in the pathogenesis of BPD. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1479898DOI Listing
February 2019

Hyperbaric oxygen rescues lung cancer cells from chemical hypoxia-induced low differentiation and apoptosis resistance.

Exp Lung Res 2018 Oct - Nov;44(8-9):417-423. Epub 2019 Feb 10.

d Laboratory of Molecular Biology and Department of Biochemistry, Key Laboratory of Gene Research of Anhui Province , Anhui Medical University , Hefei , Anhui , China.

Hypoxia induces vigorous growth and a higher malignant phenotype in solid tumors. Hyperoxic treatment using hyperbaric oxygen (HBO) has previously been shown as a highly effective method to attenuate hypoxia. We aimed to investigate the effect of HBO on hypoxia-induced malignancy of lung cancer cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2019.1571124DOI Listing
February 2019
1 Read

Notch promotes DNMT-mediated hypermethylation of Klotho leads to COPD-related inflammation.

Exp Lung Res 2018 09 26;44(7):368-377. Epub 2019 Jan 26.

a Department of Respiratory and Critical Care Medicine , General Hospital of Ningxia Medical University , Yinchuan , China.

Aim: Klotho expression significantly declines in alveolar macrophages and airway epithelial cells in chronic obstructive pulmonary disease (COPD) patients, and cigarette smoke extract dramatically inhibits the expression and secretion of α-Klotho. This suggests that the silencing of Klotho is the major factor promoting COPD related inflammatory responses. This study aims to investigate the mechanism of Klotho downregulation and its effect on the inflammatory cytokines secretion and cell apoptosis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1556749DOI Listing
September 2018

Combination of TWEAK and TGF-β1 induces the production of TSLP, RANTES, and TARC in BEAS-2B human bronchial epithelial cells during epithelial-mesenchymal transition.

Exp Lung Res 2018 09 24;44(7):332-343. Epub 2019 Jan 24.

a Department of Respiratory Medicine, Faculty of Medicine , Graduate School of Medicine, Juntendo University , Tokyo , Japan.

Aim Of The Study: In patients with asthma, chronic inflammatory processes and the subsequent remodeling of the airways contribute to the symptoms and the pathophysiological changes. Epithelial-mesenchymal transition (EMT) is thought to play an important role in tissue remodeling. Previous reports show that tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) is a cytokine of the TNF superfamily, exerts pro-inflammatory effects, and enhances transforming growth factor (TGF)-β-induced EMT in bronchial epithelial cells. Read More

View Article

Download full-text PDF

Source
https://www.tandfonline.com/doi/full/10.1080/01902148.2018.1
Publisher Site
http://dx.doi.org/10.1080/01902148.2018.1522558DOI Listing
September 2018
6 Reads

Bronchial epithelial cells produce CXCL1 in response to LPS and TNFα: A potential role in the pathogenesis of COPD.

Exp Lung Res 2018 09 24;44(7):323-331. Epub 2019 Jan 24.

a Department of Respiratory Medicine , Kyorin University School of Medicine , Mitaka , Japan.

Rationale: Neutrophilic airway inflammation plays a central role in chronic obstructive pulmonary disease (COPD). CXC chemokine ligand (CXCL)1 is a neutrophil chemokine involved in the pathogenesis of COPD. However, its clinical significance in COPD patients is poorly understood. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1520936DOI Listing
September 2018
1 Read

CB2R agonist prevents nicotine induced lung fibrosis.

Exp Lung Res 2018 09 24;44(7):344-351. Epub 2019 Jan 24.

c Chair and Department of Pharmacology and Pharmacodynamics , Medical University in Lublin , Poland.

Introduction: Nicotine stimulates fibroblast proliferation while increasing inflammation and fibrosis of tissues. The cannabinoid receptor 1 (CB1R) is mainly located in the CNS, while cannabinoid receptor 2 (CB2R) is located in the immune cells within the body. CB2R regulates inflammatory processes and fibroblast function. Read More

View Article

Download full-text PDF

Source
https://www.tandfonline.com/doi/full/10.1080/01902148.2018.1
Publisher Site
http://dx.doi.org/10.1080/01902148.2018.1543368DOI Listing
September 2018
9 Reads

Delivery of nucleic acids to ex vivo porcine airways using electrospray.

Exp Lung Res 2018 Oct - Nov;44(8-9):405-416. Epub 2019 Jan 24.

b Avectas Ltd. , Maynooth , Ireland.

Aim Of The Study: Nucleic acid-based therapies have the potential to provide clinically meaningful benefit across a wide spectrum of lung disease. However, in vivo delivery remains a challenge. Here we examined the feasibility of using electrospray to deliver nucleic acids to both porcine tracheal tissue sections and whole lung ex vivo. Read More

View Article

Download full-text PDF

Source
https://www.tandfonline.com/doi/full/10.1080/01902148.2018.1
Publisher Site
http://dx.doi.org/10.1080/01902148.2018.1563924DOI Listing
January 2019
7 Reads

Hypothermia alleviated LPS-induced acute lung injury in Rat models through TLR2/MyD88 pathway.

Exp Lung Res 2018 Oct - Nov;44(8-9):397-404. Epub 2019 Jan 19.

a Department of Critical Care Medicine , the First Affiliated Hospital of Guangxi Medical University , Nanning , Guangxi Zhuang Autonomous Region, China.

Acute lung injury (ALI) is a common clinical syndrome in ICU departments with high mortality. The pathology of ALI is still not clear and there is no specific and efficient treatment against ALI. In this study, we established ALI rat model through lipopolysaccharide administration. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1557299DOI Listing
January 2019
14 Reads

Tuberculosis in the 21th century: Current status of diagnostic methods.

Exp Lung Res 2018 09 19;44(7):352-360. Epub 2019 Jan 19.

b Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro , Vila Real , Portugal.

Tuberculosis is an infectious bacterial disease with a high mortality rate worldwide constituting a serious public health problem. The diagnostic methods commonly used by health professionals are slow and expensive and the results may take about sixty days which will cause a delay in administrating the most proper treatment to the patient, as well as increase health care costs and infection transmission possibility. Patients infected simultaneously with human immunodeficiency virus and Mycobacterium tuberculosis are a constant and worrying challenge for the scientific community which will research and develop new methods of diagnosis, new drugs and new therapies. Read More

View Article

Download full-text PDF

Source
https://www.tandfonline.com/doi/full/10.1080/01902148.2018.1
Publisher Site
http://dx.doi.org/10.1080/01902148.2018.1545880DOI Listing
September 2018
4 Reads

miR-221 participates in the airway epithelial cells injury in asthma via targeting SIRT1.

Exp Lung Res 2018 08 17;44(6):272-279. Epub 2019 Jan 17.

a Asthma Control and Prevention Center, the Gansu Provincial People's Hospital , Lanzhou , China.

Aim Of The Study: To investigate the role of microRNA-221 (miR-221) in the airway epithelial cell injury in asthma and delineate the underlying mechanism that may involve with SIRT1.

Materials And Method: Bronchial epithelial cells from asthma patients and healthy controls were obtained by bronchoscopic brushing. The miR-221 and SIRT1 mRNA level in collected cells were detected by qRT-CPR. Read More

View Article

Download full-text PDF

Source
https://www.tandfonline.com/doi/full/10.1080/01902148.2018.1
Publisher Site
http://dx.doi.org/10.1080/01902148.2018.1533051DOI Listing
August 2018
3 Reads

Evidence for angiotensin mediation of the late histopathological effects of pulmonary fat embolism: Protection by losartan in a rat model.

Exp Lung Res 2018 09 13;44(7):361-367. Epub 2019 Jan 13.

e Pathology , University of Missouri Kansas City , Kansas City , MO, USA.

Purpose: In a model of fat embolism using triolein-treated rats, we have reported that the acute pulmonary histopathological changes at 48 hrs were ameliorated by the angiotensin AT1 receptor blocker losartan, the angiotensin converting enzyme inhibitor captopril, and the direct renin inhibitor aliskiren. Although much of the pathology had declined by 3 weeks, the changes persisted at 6 weeks. The purpose of the study was to extends the time course investigation to 10 weeks and to examines whether the fat embolism effects continue to be blocked by losartan when given at a late time period. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1552339DOI Listing
September 2018
3 Reads

HMGB1 signaling blocking protects against carbapenem-resistant klebsiella pneumoniae in a murine model of infection.

Exp Lung Res 2018 08 29;44(6):263-271. Epub 2018 Dec 29.

b Department of Scientific Research and Teaching , Heze municipal Hospital , Heze , China.

Purpose Of The Study: Pulmonary infection with Klebsiella pneumoniae (KP) and carbapenem-resistant Klebsiella pneumoniae (CRKP) significantly contribute to morbidity and mortality in pneumonia. Recent studies have indicated that High-Mobility Group Box 1 Protein (HMGB1) plays an important role in the prevention and treatment of pneumonia. However the role of HMGB1 in CRKP-induced pneumonia has not been addressed. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1505976DOI Listing
August 2018
2 Reads

Metabolic regulation protects mice against Klebsiella pneumoniae lung infection.

Exp Lung Res 2018 08 4;44(6):302-311. Epub 2018 Dec 4.

b Department of Infectious Diseases , Affiliated Union Hospital of Tongji Medical College, Huazhong University of Science and Technology , Wuhan , Hubei , China.

Purpose: Klebsiella pneumoniae-caused pneumonia is a risk factor for development of lung injury. However, the current clinical isolates of K. pneumoniae are mostly multidrug-resistance and thus must be addressed with new treatments. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1538396DOI Listing
August 2018
4 Reads
1.754 Impact Factor

The effect of microRNA-129 on the migration and invasion in NSCLC cells and its mechanism.

Exp Lung Res 2018 08 26;44(6):280-287. Epub 2018 Nov 26.

a Department of Thoracic-Cardio Surgery , The Affiliated Drum Tower Hospital of Nanjing University Medical School , Nanjing , China.

Purpose: To investigate the effect of microRNA-129 on the migration and invasion in (Non small cell lung cancer) NSCLC cells and involved mechanism.

Materials And Methods: A549 cells were cultured and transfected with microRNA-129 (miR-129) mimic. Wound-healing assay and transwell assay were used to analyses the migration and invasion of A549 cells, respectively. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1536174DOI Listing
August 2018
4 Reads

LPS-induced MMP-9 expression is mediated through the MAPKs-AP-1 dependent mechanism in BEAS-2B and U937 cells.

Exp Lung Res 2018 May - Jun;44(4-5):217-225. Epub 2018 Nov 23.

a Department of Respiratory and Critical Care Medicine , Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai , PR China.

Aim Of The Study: Matrix metalloproteinases (MMPs) play a critical role in chronic obstructive pulmonary disease (COPD). This study investigated the role of mitogen-activated protein kinases (MAPKs) in MMP-9 secretion of BEAS-2B cells, a human bronchial epithelial cell line and U937 cells, a human myeloid leukaemia cell line, which could differentiate into macrophage, after LPS stimulation, and some details of involved signaling.

Materials And Methods: MTT assay was used to measure cell viability. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1493551DOI Listing
February 2019
2 Reads

Isolating and culturing of sputum macrophages: A potential ex vivo/in vitro model.

Exp Lung Res 2018 08 22;44(6):312-322. Epub 2018 Nov 22.

c Department of Respiratory Medicine , Rikshospitalet, Oslo University Hospital AND University of Oslo , Oslo , Norway.

Purpose: This paper aimed to test whether induced sputum samples acquired from human volunteers could be used to isolate and culture airway macrophages for in vitro exposures. This was assessed in terms of the culturing success rate, culture purity, viability and responsiveness of cultured cells.

Materials And Methods: The isolation and culturing procedure was performed over three days. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1539788DOI Listing
August 2018
2 Reads

Effect of gastric fluid aspiration on the lung microbiota of laboratory rats.

Exp Lung Res 2018 May - Jun;44(4-5):201-210. Epub 2018 Nov 22.

a Department of Surgery , Duke University Medical Center , Durham , North Carolina , USA.

Aim Of The Study: The pulmonary microbiota is important for both normal homeostasis and the progression of disease, and may be affected by aspiration of gastric fluid. The aim of this study was to investigate changes in the lung microbiota induced by aspiration of gastric fluid in a laboratory rat model.

Material And Methods: Using the intratracheal application method, male rats received aspiration with 0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1482976DOI Listing
February 2019
14 Reads

NF-κB p65 Knock-down inhibits TF, PAI-1 and promotes activated protein C production in lipopolysaccharide-stimulated alveolar epithelial cells type II.

Exp Lung Res 2018 May - Jun;44(4-5):241-251. Epub 2018 Nov 19.

a Department of Critical Care Medicine , The Affiliated Hospital of Guizhou Medical University , Guiyang , China.

Purpose/aim: Activated coagulation and reduced fibrinolysis in alveolar compartment are an important characteristics in acute respiratory distress syndrome (ARDS). Alveolar epithelial cell type II (AECII) participates in regulating the intra-alveolar abnormalities of coagulation and fibrinolysis mainly through adjusting the productions of tissue factor (TF), plasminogen activator inhibitor (PAI)-1 and activated protein C (APC) in ARDS. NF-κB signal pathway may be involved in coagulation regulation in sepsis-induced ALI. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1505975DOI Listing
February 2019
24 Reads

TSLP promotes asthmatic airway remodeling via p38-STAT3 signaling pathway in human lung fibroblast.

Exp Lung Res 2018 08 14;44(6):288-301. Epub 2018 Nov 14.

a Department of Pulmonary Diseases , Qilu Hospital, Shandong University , Jinan , Shandong , People's Republic of China.

Purpose: Thymic stromal lymphopoietin (TSLP) acts as a critical cytokine involved in asthmatic airway remodeling. Our study aimed to characterize the crosstalk between airway epithelial cells and fibroblasts regulated by TSLP through the signaling pathways of Mitogen-activated protein kinase (MAPK) and signal transducer and activator of transcription 3 (STAT3).

Materials And Methods: Human biopsy specimens and lung tissues from mice were stained with hematoxylin and eosin (H&E) and immunohistochemistry. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1536175DOI Listing
August 2018
13 Reads
1.754 Impact Factor

Total emitted dose of salbutamol sulphate at different inhalation flows and inhalation volumes through different types of dry powder inhalers.

Exp Lung Res 2018 May - Jun;44(4-5):211-216. Epub 2018 Oct 22.

g Clinical Pharmacy Department, Faculty of Pharmacy , Ahram Canadian University , Giza , Egypt.

The aim of the present study was to compare the performance of two different dry powder inhalers (DPIs) at different inhalations volumes and inhalation flows. Ventolin Diskus contain blisters of 200µg salbutamol. To test the TED from Aerolizer, salbutamol in Diskus blister was emptied and placed in size 3 capsules suitable for use with Aerolizer. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1489015DOI Listing
February 2019
10 Reads

Repeated pulmonary exposures to zinc ions enhance inflammatory responses to subsequent metal exposures.

Exp Lung Res 2018 May - Jun;44(4-5):252-261. Epub 2018 Oct 8.

a Molecular and Integrative Physiological Sciences Program, Department of Environmental Health , Harvard T.H. Chan School of Public Health , Boston , Massachusetts , USA.

Aim Of Study: Metal contaminants contribute to adverse human health effects via acute and chronic exposures. Acute metal exposures followed by prolonged secondary metal exposures may elicit exaggerated inflammatory responses in certain individuals. The aim of this study is to determine whether repeated pulmonary exposures to zinc chloride (ZnCl) alter subsequent responses to zinc or cerium exposures. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1517837DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351161PMC
February 2019
1 Read

Lipopolysaccharide-induced chorioamnionitis and postnatal lung injury: The beneficial effects of L-citrulline in newborn rats.

Exp Lung Res 2018 May - Jun;44(4-5):226-240. Epub 2018 Sep 10.

a Neonatal Intensive Care Unit, Women's and Children's Health Department , University of Padova , Padova , Italy.

Aim Of The Study: The lung architecture of newborns appears to be affected by an inflammatory reaction to maternal choriodecidual layer infection. L-citrulline (L-Cit) was administered to pregnant rats exposed to intra-amniotic lipopolysaccharide (LPS)-induced chorioamnionitis to investigate its effect on neonatal lung injury.

Materials And Methods: The pups were assigned to four experimental groups: 1- pups exposed to intra-amniotic NaCl but not to postnatal L-Cit (Controls); 2 - pups exposed to intra-amniotic NaCl as well as to postnatal L-Cit treatment (L-Cit group); 3 - pups exposed to prenatal LPS but not to postnatal (LPS); 4- pups exposed to prenatal LPS as well as to postnatal L-Cit treatment (LPS + L-Cit). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1497730DOI Listing
February 2019
12 Reads

Changes in mucin 1 expression in a rat model of allergic airway inflammation.

Exp Lung Res 2018 04 30;44(3):137-142. Epub 2018 May 30.

b Xinjiang Medical University, Basic Medical College , Histology and Embryology , Urumqi , China.

Purpose: To explore the distribution and expressional changes of mucin 1 (Muc1) in airway of rats with allergic airway inflammation.

Materials And Methods: Ovalbumin (OVA) was used to induce acute allergic inflammation in male Wistar rats. The distributions and expressions of Muc1 in lungs of normal and model rats were assessed by immunohistochemical staining and western blotting, respectively. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1439127DOI Listing
April 2018
2 Reads

The effect of captopril on lipopolysaccharide-induced lung inflammation.

Exp Lung Res 2018 May - Jun;44(4-5):191-200. Epub 2018 May 30.

a Neurogenic Inflammation Research Centre, Mashhad University of Medical Sciences , Mashhad , Iran.

Purpose: As an angiotensin converting enzyme (ACE) inhibitor, the effects of captopril on inflammation has been previously examined. Captopril has been shown to have anti-inflammatory and antioxidant effects. Imbalance in the oxidant/antioxidant system is one of the major causes of inflammation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1473530DOI Listing
February 2019
6 Reads

Sialylation pattern in lung epithelial cell line and Siglecs expression in monocytic THP-1 cells as cellular indicators of cigarette smoke - induced pathology in vitro.

Exp Lung Res 2018 04 21;44(3):167-177. Epub 2018 May 21.

a Department of Clinical Pharmacology , Medical University of Bialystok , Bialystok , Poland.

Purpose: Cellular response to cigarette smoke (CS) involves activation of recognition receptors resulting in changes in immune status, oxidative stress and cell turnover. We investigated the effects of CS on sialic acid-binding immunoglobulin type lectins (Siglecs) expression and their sialylated ligands in human immune and non-immune cells.

Methods: Human monocytes (THP-1) and epithelial cells (A549) were cultured in CS-conditioned medium (CSM). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1461959DOI Listing
April 2018
23 Reads

Rux largely restores lungs in Iraq PM-exposed mice, Up-regulating regulatory T-cells (Tregs).

Exp Lung Res 2018 04 8;44(3):153-166. Epub 2018 May 8.

k Department of Occupational and Environmental Medicine , Preventive Medicine, and Epidemiology (Population Health), Donald and Barbara Zucker School of Medicine at Hofstra/Northwell , Hempstead , NY.

Background Military personnel post-deployment to Iraq and Afghanistan have noted new-onset respiratory illness. This study's primary objective was to further develop an animal model of Iraq Afghanistan War Lung Injury (IAW-LI) and to test a novel class of anti-injury drug called RuX. Methods Particulate Matter (PM) samples were obtained in Iraq then characterized by spectromicroscopy. Read More

View Article

Download full-text PDF

Source
https://www.tandfonline.com/doi/full/10.1080/01902148.2018.1
Publisher Site
http://dx.doi.org/10.1080/01902148.2018.1459957DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6462226PMC
April 2018
37 Reads

MicroRNAs in idiopathic pulmonary fibrosis, new research progress and their pathophysiological implication.

Exp Lung Res 2018 04 23;44(3):178-190. Epub 2018 Apr 23.

c Fourth Affiliated Hospital, Anhui Medical University , Hefei , China.

Many studies have shown that microRNAs (miRNAs) play important roles in the development of idiopathic pulmonary fibrosis (IPF). The purpose of this review is to systematically summarize the recent advance of miRNAs in the pathology of IPF, highlighting the new research progress and their pathophysiological implication. Recent studies have shown that miRNAs differentially expressed in blood and lung tissue from IPF patients are closely related to the occurrence of IPF disease, which may be IPF diagnostic markers and prognostic indicators. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1455927DOI Listing
April 2018
2 Reads

Bronchiolitis obliterans murine model induced by nitric acid aerosol inhalation: An economical and reproducible model.

Exp Lung Res 2018 04 23;44(3):143-152. Epub 2018 Apr 23.

a Department of Pediatrics , The First Hospital of Jilin University , Changchun , jilin , China.

Purpose: Bronchiolitis obliterans (BO) is a highly debilitative and fatal syndrome associated with a series of severe lower airway disorders. The pathogenesis of BO is complicated and not entirely understood. An appropriate animal model of BO may aid research into its pathogenesis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1455926DOI Listing
April 2018
7 Reads

Improved protocol for simultaneous analysis of leukocyte subsets and epithelial cells from murine and human lung.

Exp Lung Res 2018 04 20;44(3):127-136. Epub 2018 Apr 20.

a Department of Pediatrics, Allergology, and Neonatology , Hannover Medical School , Hannover , Germany.

Purpose: To study and isolate lung cells by flow cytometry, enzymatic digestion and generation of single cell suspensions is required. This significantly influences expression of cellular epitopes and protocols need to be adapted for the best isolation and subsequent analysis of specific cellular subsets.

Materials And Methods: We optimized protocols for the simultaneous isolation and characterization of specific human and murine lung cell types. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1432721DOI Listing
April 2018
44 Reads

Regulation of eosinophil recruitment and allergic airway inflammation by heparan sulfate proteoglycan (HSPG) modifying enzymes.

Exp Lung Res 2018 03 5;44(2):98-112. Epub 2018 Apr 5.

a Departments of Veterinary and Biomedical Sciences.

Background: HSPGs are glycoproteins containing covalently attached heparan sulfate (HS) chains which bind to growth factors, chemokines, etc., and regulate various aspects of inflammation including cell recruitment. We previously showed that deletion of endothelial N-acetylglucosamine N-deacetylase-N-sulfotransferase-1 (Ndst1), an enzyme responsible for N-sulfation during HS biosynthesis, reduces allergic airway inflammation (AAI). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1451574DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6166647PMC
March 2018
6 Reads

Tuberculosis and cigarette smoke exposure: An update of in vitro and in vivo studies.

Exp Lung Res 2018 03 22;44(2):113-126. Epub 2018 Mar 22.

c Departamento de Patologia, Unidad de Patologia Experimental , Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran , Mexico.

Tuberculosis (TB) has been declared the first cause of death by an infectious agent. Annually, 10.4 million people suffer active TB. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1444824DOI Listing
March 2018
3 Reads

MicroRNA-21 aggravates chronic obstructive pulmonary disease by promoting autophagy.

Exp Lung Res 2018 03 15;44(2):89-97. Epub 2018 Mar 15.

a Center for Experimental Medical Research , the Third Xiangya Hospital of Central South University , Changsha , Hunan , P.R. China.

MicroRNAs and autophagy play important roles in chronic obstructive pulmonary disease (COPD). This study was designed to explore the role of microRNA-21 (miR-21) induced autophagy in COPD. Using the C57BL/6, miR-21-/- mice and human bronchial epithelial (16HBE) cell line, we found that in the lung tissues of mice, the level of autophagy in the COPD model group was significantly higher than that in the control group. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2018.1439548DOI Listing
March 2018
4 Reads

Emphysema induced by elastase alters the mRNA relative levels from DNA repair genes in acute lung injury in response to sepsis induced by lipopolysaccharide administration in Wistar rats.

Exp Lung Res 2018 03 8;44(2):79-88. Epub 2018 Feb 8.

a Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes , Universidade do Estado do Rio de Janeiro , Vila Isabel , Rio de Janeiro , Brazil.

Purpose/Aim of the study: Patients suffering from chronic obstructive pulmonary disease (COPD) in association with acute respiratory distress syndrome (ARDS) present oxidative stress in lung cells, with production of free radicals and DNA lesions in pulmonary and adjacent cells. Once the DNA molecule is damaged, a set of enzymatic mechanisms are trigged to preserve genetic code integrity and cellular homeostasis. These enzymatic mechanisms include the base and the nucleotide excision repair pathways, as well as telomere regulation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2017.1422158DOI Listing
March 2018
4 Reads

Kaempferol attenuates acute lung injury in caecal ligation and puncture model of sepsis in mice.

Exp Lung Res 2018 03 2;44(2):63-78. Epub 2018 Feb 2.

a Division of Pharmacology and Toxicology , ICAR-Indian Veterinary Research Institute , Bareilly , Uttar Pradesh , India.

Aim Of The Study: Kaempferol is a flavonoid and important part of the diet. Kaempferol has shown antioxidant, antiinflammatory and antidiabetic activities in various studies. However, protective potential of kaempferol in acute lung injury induced by sepsis and its mechanism remains unclear. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2017.1420271DOI Listing
March 2018
9 Reads
1.750 Impact Factor

Low dose monocrotaline causes a selective pulmonary vascular lesion in male and female pneumonectomized rats.

Exp Lung Res 2018 02 30;44(1):51-61. Epub 2018 Jan 30.

a Aab Cardiovascular Research Institute, University of Rochester Medical Center , Rochester , New York , USA.

Purpose/Aim: Low doses (30-80 mg/kg) of monocrotaline are commonly used to create experimental models of pulmonary hypertension in rats. At these doses, monocrotaline causes pulmonary endothelial apoptosis and acute lung injury which ultimately results in pulmonary vascular disease. Higher doses of monocrotaline (300 mg/kg) are known to create severe liver injury, but previous investigations with lower doses have not reported histology in other organs to determine whether the vascular injury with monocrotaline is pulmonary-selective or generalized. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2017.1422157DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240164PMC
February 2018
21 Reads
1.754 Impact Factor

Erratum.

Authors:
Yun Zeng

Exp Lung Res 2018 02 26;44(1):62. Epub 2018 Jan 26.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2017.1422369DOI Listing
February 2018
5 Reads

Granulomatous lung inflammation is nanoparticle type-dependent.

Exp Lung Res 2018 02 11;44(1):25-39. Epub 2018 Jan 11.

a Inflammation and Immunity of the Respiratory Epithelium 1 - EA7426, Faculté de médecine Lyon Sud , UCBL1, Pierre Benite , France.

Background: Nanoparticles are increasingly suspected as a strong etiologic factor of granuloma formation.

Aim Of The Study: The aim of our study was to compare lung inflammatory response and histology changes following exposure of mice to two widely used nanoparticles: carbon nanotubes (MWCNT) and cadmium-based nanoparticles (QDOT705) in an attempt to better our understanding of granulomatous inflammation.

Materials And Methods: Various groups of mice were included: control mice and mice that were intranasally instilled with QDOT or MWCNT. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2017.1412541DOI Listing
February 2018
11 Reads

Four-day pulse of sodium cromoglycate modulates pulmonary vessel wall remodeling during 21-day hypoxia in rats.

Exp Lung Res 2018 02 11;44(1):1-12. Epub 2018 Jan 11.

a Department of Histology and Embryology, Second Faculty of Medicine , Charles University in Prague , Plzeňska , Prague , Czech Republic.

Aim Of The Study: Remodeling of pulmonary resistance arteries in rats due to 4-day hypoxia could be successfully suppressed by sodium cromoglycate. In this study, we tested the difference in the suppression between two distinct time patterns of cromoglycate administration during 21-day hypoxia. In the experiment, we focused on some details in both smooth muscle cells and extracellular matrix of pulmonary arterial walls. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2017.1393708DOI Listing
February 2018
7 Reads

The expression profile of Claudin family members in the developing mouse lung and expression alterations resulting from exposure to secondhand smoke (SHS).

Exp Lung Res 2018 02 11;44(1):13-24. Epub 2018 Jan 11.

a Department of Physiology and Developmental Biology, Lung and Placenta Research Laboratory , Brigham Young University , Provo , Utah , USA.

Claudins are tight junctional proteins implicated in cell polarity and epithelial barrier maintenance. Claudin misregulation adversely impacts developmental aspects of cell differentiation and proliferation. The current research evaluated transcriptional expression for Claudins 1-11 and 18 in the developing murine lung at embryonic days (E) 14. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2017.1409846DOI Listing
February 2018
8 Reads

Anti-IL-8 antibody potentiates the effect of exogenous surfactant in respiratory failure caused by meconium aspiration.

Exp Lung Res 2018 02 11;44(1):40-50. Epub 2018 Jan 11.

a Biomedical Center Martin and Department of Physiology , Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava , Martin , Slovakia.

Aim: Meconium aspiration syndrome (MAS) is life-threatening respiratory failure of newborns which can be treated by exogenous surfactant. In response to meconium, increased levels of chemokine IL-8 (CXCL8) stimulate massive neutrophil infiltration of the lungs. Local accumulation and activation of neutrophils, on-going inflammation, lung edema, and oxidative damage contribute to inactivation of endogenous and therapeutically given surfactants. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2017.1420272DOI Listing
February 2018
7 Reads

Murine model of oropharyngeal gastric fluid aspiration-A new assessment method for intrapulmonary liquid distribution using digital pixel calculation.

Exp Lung Res 2017 Nov - Dec;43(9-10):434-438

a Department of Surgery , Duke University Medical Center , Durham , North Carolina , USA.

Aim Of The Study: The aim of this study was to investigate a new method for visualization and quantification of intrapulmonary liquid distribution after oropharyngeal gastric fluid aspiration in mice.

Material And Methods: Eleven mice received oropharyngeal aspiration with a gastric fluid, India ink, and saline solution. Digital imaging and pixel calculation were used to analyze intrapulmonary fluid distribution selectively. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2017.1397822DOI Listing
August 2018
18 Reads

Budesonide instillation immediately after reperfusion ameliorates ischemia/reperfusion-induced injury in the transplanted lung of rat.

Exp Lung Res 2017 Nov - Dec;43(9-10):439-446. Epub 2017 Dec 13.

a Department of Anesthesiology , The Second Affiliated Hospital of the Harbin Medical University , Harbin , Heilongjiang Province , China.

Purpose: Lung ischemia-reperfusion injury (LIRI) after lung transplantation can lead to primary graft dysfunction. Budesonide can improve endothelial function to reduce lung injury. This study was aimed to examine the effects of budesonide on LIRI and potential mechanisms. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01902148.2017.1405103DOI Listing
August 2018
6 Reads