3,658 results match your criteria Experimental Dermatology[Journal]


Naringenin as an opener of mitochondrial potassium channels in dermal fibroblasts.

Exp Dermatol 2019 Feb 18. Epub 2019 Feb 18.

Department of Biophysics, Warsaw, University of Life Sciences - SGGW, Warsaw, Poland.

Flavonoids belong to a large group of polyphenolic compounds that are widely present in plants. Certain flavonoids, including naringenin, have cytoprotective properties. Although the antioxidant effect has long been thought to be a crucial factor accounting for the cellular effects of flavonoids, mitochondrial channels have emerged recently as targets for cytoprotective strategies. Read More

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http://dx.doi.org/10.1111/exd.13903DOI Listing
February 2019

Imaging of pH and pO gives insight in molecular processes of irradiated cells.

Exp Dermatol 2019 Feb 18. Epub 2019 Feb 18.

Department of Otorhinolaryngology, Ludwig-Maximilians-University Munich, 81377, Munich, Germany.

One of the major challenges in radiation therapy is the interference with tissue repair processes due to hypoxic characteristics and pH dysregulation. In this study, we present dual imaging of pH and oxygenation in vitro based on luminescent biocompatible sensor foils that allow studying the effects of irradiation on different cell types in culture. Different sensitivities of fibroblast and oral squamous carcinoma cells were observed by complementing oxygen and pH differences with proliferation assays. Read More

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http://dx.doi.org/10.1111/exd.13905DOI Listing
February 2019

Do human dermal adipocytes switch from lipogenesis in anagen to lipophagy and lipolysis during catagen in the human hair cycle?

Exp Dermatol 2019 Feb 18. Epub 2019 Feb 18.

Centre for Dermatology Research, The University of Manchester, Manchester, UK.

In murine skin dermal white adipose tissue (DWAT) undergoes fluctuations in size across the hair cycle, whereas changes in size, function and metabolism of dermal adipocytes (DAs) during the human scalp hair cycle remain unexplored. Transmission electron microscopy results suggest that, during anagen-catagen transition, human DAs co-opt the autophagy machinery to undergo lipophagy within their lipid droplets. Wholemount staining of hair follicles (HFs) and surrounding DWAT for the autophagy marker LC3B confirms the increased presence of LC3B+ lipid droplets adjacent to catagen HFs; moreover, DWAT around catagen HFs engages in greater glycerol release compared to DWAT surrounding anagen HFs. Read More

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http://dx.doi.org/10.1111/exd.13904DOI Listing
February 2019

Topical kinase inhibitors induce regression of cutaneous squamous cell carcinoma.

Exp Dermatol 2019 Feb 14. Epub 2019 Feb 14.

Department of Dermatology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, 19104.

Actinic keratoses (AKs) and squamous cell carcinoma in situ (SCCIS) are precursor lesions for cutaneous squamous cell carcinoma (cSCC), the second most common form of cancer. Current topical therapies for AKs and SCCIS promote skin inflammation to eradicate lesions and do not directly target the biological mechanisms driving growth. We hypothesized that topical small molecule inhibitors targeting kinases promoting keratinocyte growth in AKs and SCCIS could induce regression of these lesions with less inflammation. Read More

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http://doi.wiley.com/10.1111/exd.13902
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http://dx.doi.org/10.1111/exd.13902DOI Listing
February 2019
4 Reads

Dissection of Merkel cell formation in hairy and glabrous skin reveals a common requirement for FGFR2-mediated signaling.

Exp Dermatol 2019 Feb 13. Epub 2019 Feb 13.

Black Family Stem Cell Institute, The Tisch Cancer Institute, Department of Cell, Developmental, and Regenerative Biology, Icahn School of Medicine at Mount Sinai, 1 Gustave L. Levy Place, New York, NY, 10029, USA.

Merkel cells are mechanosensory cells involved in tactile discrimination. Merkel cells have been primarily studied in the murine back skin, where they are found in specialized structures called touch domes located around primary hair follicles. Yet, little is known about the morphogenesis of Merkel cells in areas of the skin devoid of hair, such as the glabrous paw skin. Read More

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http://dx.doi.org/10.1111/exd.13901DOI Listing
February 2019

A potential contribution of decreased galectin-7 expression in stratified epithelia to the development of cutaneous and esophageal manifestations in systemic sclerosis.

Exp Dermatol 2019 Feb 9. Epub 2019 Feb 9.

Department of Dermatology, University of Tokyo Graduate School of Medicine, Tokyo, 113-8655, Japan.

Backgrounds: Stratified epithelia have caught much attention as potential contributors to the development of dermal and esophageal fibrosis in systemic sclerosis (SSc). Galectin-7 is a marker of all types of stratified epithelia, which is involved in the maintenance of epidermal homeostasis. So far, the role of galectin-7 has not been studied in SSc. Read More

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http://dx.doi.org/10.1111/exd.13900DOI Listing
February 2019
1 Read

Comparative regenerative biology of spiny (Acomys cahirinus) and laboratory (Mus musculus) mouse skin.

Exp Dermatol 2019 Feb 8. Epub 2019 Feb 8.

Department of Pathology, School of Medicine, University of Southern California, Los Angles, California, USA.

Wound-induced hair follicle neogenesis (WIHN) has been demonstrated in laboratory mice (Mus musculus) after large (>1.5 x 1.5 cm ) full-thickness wounds. Read More

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http://dx.doi.org/10.1111/exd.13899DOI Listing
February 2019

Wanted, dead and alive; why a multidisciplinary approach is needed to unlock hair treatment potential.

Exp Dermatol 2019 Feb 1. Epub 2019 Feb 1.

Skin Research Institute Singapore, Singapore.

Human recorded history is littered with attempts to improve the perceived appearance of scalp hair. Throughout history treatments have included both biological and chemical interventions. Hair "quality" or "perceived appearance" is regulated by multiple biological intervention opportunities: adding more hairs by flipping follicles from Telogen to Anagen, or delaying Anagen follicles transiting into Catagen; altering hair "apparent amount" by modulating shaft diameter or shape; or, in principle, altering shaft physical properties changing its synthesis. Read More

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http://dx.doi.org/10.1111/exd.13898DOI Listing
February 2019
1 Read

IL-17A/F in Leishmania major-resistant C57BL/6 mice.

Exp Dermatol 2019 Jan 31. Epub 2019 Jan 31.

Department of Dermatology, University Hospital of the Johannes Gutenberg-University Mainz, Langenbeckstraße 1, 55131, Mainz, Germany.

Proinflammatory IL-17 plays an important role in various diseases and defense against extracellular microorganisms. Healing of leishmaniasis is promoted by Th1/Tc1 cells, whereas Th2/Treg are associated with worsened disease outcome. In addition, high expression of IL-17A in Leishmania-susceptible BALB/c and artificial overexpression of IL-17A in T cells in resistant C57BL/6 mice worsened disease outcome. Read More

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http://dx.doi.org/10.1111/exd.13896DOI Listing
January 2019
1 Read

Skin equivalent assay: an optimized method for testing for hair growth reconstitution capacity of epidermal and dermal cells.

Exp Dermatol 2019 Jan 31. Epub 2019 Jan 31.

Department of Dermatology, Seoul National University College of Medicine, Seoul, Republic of Korea.

Hair follicle reconstitution requires highly organized epithelial-mesenchymal interactions. Skin equivalents containing the epidermal and dermal cells with hair reconstitution capacity can reproduce these processes, but have not been established. This study was conducted to develop a hair follicle-producing three-dimensional (3D) skin equivalent assay using neonate mouse epidermal and dermal cells. Read More

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http://dx.doi.org/10.1111/exd.13897DOI Listing
January 2019
1 Read

Basic analysis of facial ageing: The relationship between the superficial musculoaponeurotic system and age.

Exp Dermatol 2019 Feb;28 Suppl 1:38-42

Department of Clinical Anatomy, Tokyo Medical and Dental University (TMDU), Tokyo, Japan.

Background: The superficial musculoaponeurotic system (SMAS) is an anatomical structure involved in the facial ageing process. We aimed to evaluate the SMAS laxity using multi-detector row computed tomography (MDCT) as a diagnostic tool, and to analyse the relationship between SMAS laxity and age.

Methods: Fifty-seven female subjects (aged 21-57 years) were enrolled in the study. Read More

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http://dx.doi.org/10.1111/exd.13827DOI Listing
February 2019

Mechanisms of repigmentation induced by photobiomodulation therapy in vitiligo.

Exp Dermatol 2019 Feb;28 Suppl 1:10-14

Department of Dermatology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.

Photobiomodulation (PBM) therapy is based on the exposure of biological tissues to low-level laser light (coherent light) or light-emitting diodes (LEDs; noncoherent light), leading to the modulation of cellular functions, such as proliferation and migration, which result in tissue regeneration. PBM therapy has important clinical applications in regenerative medicine. Vitiligo is an acquired depigmentary disorder resulting from disappearance of functional melanocytes in the involved skin. Read More

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http://dx.doi.org/10.1111/exd.13823DOI Listing
February 2019
6 Reads

Facial distribution of squamous cell carcinoma in Japanese.

Exp Dermatol 2019 Feb;28 Suppl 1:72-74

Department of Geriatric and Environmental Dermatology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.

Squamous cell carcinoma (SCC) is a malignant tumor of the skin. SCC is frequently distributed on highly exposed areas such as the face and dorsal surface of the hands because it is closely related to ultraviolet damage. We retrospectively analyzed 106 cases of SCC treated at Nagoya City University Hospital from April 2004 to October 2015 and examined regional features based on modified facial aesthetic units. Read More

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http://dx.doi.org/10.1111/exd.13830DOI Listing
February 2019
3 Reads

Novel nanocapsule of α-lipoic acid reveals pigmentation improvement: α-Lipoic acid stimulates the proliferation and differentiation of keratinocyte in murine skin by topical application.

Exp Dermatol 2019 Feb;28 Suppl 1:55-63

Department of Research and Development, Nanoegg Research Laboratories, Inc. Advanced Medicine and Healthcare Research Laboratories, Kawasaki-shi, Kanagawa, Japan.

α-Lipoic acid is amphipathic with low molecular sulphur-containing fatty acid and has strong antioxidant effects. It has been used at the purposes of anti-ageing, treatment of diabetic neuropathy, and supplement as antioxidant. Though α-lipoic acid is normally administered in oral or injection, it has not been used in a topical use via skin because of its bad penetration. Read More

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http://dx.doi.org/10.1111/exd.13828DOI Listing
February 2019
3 Reads

Preface.

Exp Dermatol 2019 Feb;28 Suppl 1

Department of Geriatric and Environmental Dermatology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.

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http://dx.doi.org/10.1111/exd.13817DOI Listing
February 2019

Placental extracts regulate melanin synthesis in normal human melanocytes with alterations of mitochondrial respiration.

Exp Dermatol 2019 Feb;28 Suppl 1:50-54

Department of Applied Chemistry and Biotechnology, Okayama University of Science, Okayama, Japan.

Placental extracts have been widely used as skin lightening agents in the Japanese cosmetic market. Here, we show that placental extracts contain factors that can decrease or increase melanin synthesis by normal human melanocytes in vitro in possible association with mitochondrial respiration. When normal human melanocytes were treated with a whole porcine placental extract, melanin synthesis was decreased. Read More

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http://dx.doi.org/10.1111/exd.13824DOI Listing
February 2019
4 Reads

3-O-Laurylglyceryl ascorbate improves the development of sensitive skin through the reduction of oxidative stress.

Exp Dermatol 2019 Feb;28 Suppl 1:64-68

School of Bioscience and Biotechnology, Tokyo University of Technology, Hachiouji, Tokyo, Japan.

Skin sensitivity is a serious problem for many people, and it can be induced by various factors such as UV irradiation, physical and mental stresses, air pollution, dry air and so on. Skin dryness triggered by UV and dry air is one of the most important causes inducing the development of sensitive skin, and it has been reported that oxidative stress contributes to skin dryness. In this study, we investigated whether treatment with 3-O-laurylglyceryl ascorbate (VC-3LG), which is an amphipathic ascorbic acid derivative, can suppress the development of sensitive skin. Read More

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http://dx.doi.org/10.1111/exd.13820DOI Listing
February 2019
1 Read

Facial site distribution of basal cell carcinoma in Japanese.

Exp Dermatol 2019 Feb;28 Suppl 1:69-71

Department of Geriatric and Environmental Dermatology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.

Basal cell carcinoma (BCC) occurs preferentially on the face. We retrospectively analyzed 200 cases of BCC treated at Nagoya City University Hospital from April 2004 to October 2015 and examined regional features based on modified facial aesthetic units. BCC occurred more frequently on the cheek, nasal and orbital areas. Read More

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http://dx.doi.org/10.1111/exd.13829DOI Listing
February 2019

Effects and interactions of increased environmental temperature and UV radiation on photoageing and photocarcinogenesis of the skin.

Authors:
Cheng-Che E Lan

Exp Dermatol 2019 Feb;28 Suppl 1:23-27

Department of Dermatology, Kaohsiung Medical University Hospital, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.

Solar radiation is one of fundamental elements sustaining and maintaining life on earth. Previous studies on health effects from the sun exposure mostly focused on ultraviolet (UV) radiation. Although exposure to the solar radiation likely occurs in an environment with elevated temperature, the effects and interactions of elevated environmental temperature with UV radiation on the skin, especially in the context of ageing and carcinogenesis, have not been carefully examined. Read More

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http://dx.doi.org/10.1111/exd.13818DOI Listing
February 2019
6 Reads

The beneficial role of functional food components in mitigating ultraviolet-induced skin damage.

Authors:
Masashi Morifuji

Exp Dermatol 2019 Feb;28 Suppl 1:28-31

Food Microbiology Research Labs, Meiji Co., Ltd., Meiji Innovation Center, Hachiouji, Tokyo, Japan.

Excessive exposure to ultraviolet (UV) radiation can chemically alter biological molecules and is one of the major environmental health risks with potential to damage the structure and function of the skin. Numerous dietary supplements are known to optimize the skin's defenses against radiation exposure. Several studies in which the beneficial roles of functional food components, that can protect against UV-induced skin damage, have been demonstrated. Read More

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http://dx.doi.org/10.1111/exd.13825DOI Listing
February 2019
2 Reads

The impact of carbonylated proteins on the skin and potential agents to block their effects.

Exp Dermatol 2019 Feb;28 Suppl 1:32-37

School of Bioscience and Biotechnology, Tokyo University of Technology, Hachioji, Tokyo, Japan.

Carbonylated proteins (CPs) are synthesized by reactions between amino groups in proteins and reactive aldehyde compounds (RAC) yielded from lipid peroxidation initiated by reactive oxygen species (ROS). In the skin, CPs are detected in a higher frequency at sun-exposed sites of the skin in elderly subjects. Since CPs in the stratum corneum (SC) have been reported to correlate with skin water content and transepidermal water loss, it is considered that the accumulation of CPs in the SC involves the loss of skin moisture functions. Read More

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http://dx.doi.org/10.1111/exd.13821DOI Listing
February 2019
1 Read

Nicotinamide for photoprotection and skin cancer chemoprevention: A review of efficacy and safety.

Exp Dermatol 2019 Feb;28 Suppl 1:15-22

Discipline of Dermatology, Bosch Institute, University of Sydney and Royal Prince Alfred Hospital Sydney, Sydney, NSW, Australia.

Nicotinamide is a water-soluble vitamin B3 derivative that has many roles in medicine. This review examines the role of nicotinamide in dermatology and its actions in preventing photoageing and skin cancers in humans. Nicotinamide prevents ultraviolet radiation (UV) from reducing ATP levels and inhibiting glycolysis, thus preventing the UV radiation-induced energy crisis. Read More

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http://dx.doi.org/10.1111/exd.13819DOI Listing
February 2019
5 Reads

How stiff is skin?

Exp Dermatol 2019 Feb;28 Suppl 1:4-9

Division of Cell Matrix Biology & Regenerative Medicine, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, Manchester, UK.

The measurement of the mechanical properties of skin (such as stiffness, extensibility and strength) is a key step in characterisation of both dermal ageing and disease mechanisms and in the assessment of tissue-engineered skin replacements. However, the biomechanical terminology and plethora of mathematical analysis approaches can be daunting to those outside the field. As a consequence, mechanical studies are often inaccessible to a significant proportion of the intended audience. Read More

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http://dx.doi.org/10.1111/exd.13826DOI Listing
February 2019
1 Read

Pyridoxine (VB ) restores the down-regulation of serine palmitoyltransferase mRNA expression in keratinocytes cultured in highly oxidative conditions through enhancement of the intracellular antioxidant system.

Exp Dermatol 2019 Feb;28 Suppl 1:43-49

School of Bioscience and Biotechnology, Tokyo University of Technology, Tokyo, Japan.

Background: Pyridoxine (VB ), which acts as a coenzyme in the biosynthesis of niacin, is formulated in pharmaceuticals to treat skin roughness. However, the mechanism of action of VB is not known precisely.

Objective: This study was conducted to clarify the influence of highly oxidative conditions on the expression of skin moisture-related mRNAs and to evaluate the preventive effects of VB focusing on antioxidant behaviour. Read More

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http://dx.doi.org/10.1111/exd.13822DOI Listing
February 2019
2 Reads

Clinical Snippets.

Authors:

Exp Dermatol 2019 Feb;28(2)

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http://dx.doi.org/10.1111/exd.13884DOI Listing
February 2019

Adipose-derived stem cells attenuate atopic dermatitis-like skin lesions in NC/Nga mice.

Exp Dermatol 2019 Jan 28. Epub 2019 Jan 28.

Department of Plastic and Reconstructive Surgery, Seoul National University Boramae Medical Center, Seoul, 07061, Republic of Korea.

There is an unmet need in novel therapeutics for atopic dermatitis (AD). We examined the effects of autologous adipose-derived stem cells (ADSCs) on AD-like skin lesions induced by the application of 2,4-dinitrochlorobenzene (DNCB) in NC/Nga mice. Autologous ADSCs and ADSC-conditioned medium (ADSC-CM) were injected intralesionally three times. Read More

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http://dx.doi.org/10.1111/exd.13895DOI Listing
January 2019

Self-organizing hair peg-like structures from dissociated skin progenitor cells: New insights for human hair follicle organoid engineering and Turing patterning in an asymmetric morphogenetic field.

Exp Dermatol 2019 Jan 25. Epub 2019 Jan 25.

Department of Pathology, Keck School of Medicine of the University of Southern California, Los Angeles, CA.

Human skin progenitor cells will form new hair follicles, although at a low efficiency, when injected into nude mouse skin. To better study and improve upon this regenerative process, we developed an in vitro system to analyze the morphogenetic cell behavior in detail and modulate physical-chemical parameters to more effectively generate hair primordia. In this three-dimensional culture, dissociated human neonatal foreskin keratinocytes self-assembled into a planar epidermal layer while fetal scalp dermal cells coalesced into stripes, then large clusters, and finally small clusters resembling dermal condensations. Read More

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http://dx.doi.org/10.1111/exd.13891DOI Listing
January 2019

Mesenchymal stem cells in psoriatic lesions affect the skin microenvironment through circular RNA.

Exp Dermatol 2019 Jan 21. Epub 2019 Jan 21.

Institute of Dermatology, Taiyuan City Centre Hospital, Shanxi Key Laboratory of Stem Cells for Immunological Dermatosis, No. 1 Dong San Dao Xiang, Taiyuan, Shanxi Province, 030009, China.

Psoriasis is an autoimmune skin disease. Our previous studies revealed abnormal immune regulation of skin mesenchymal stem cells (S-MSCs) in psoriatic lesions. Circular RNA (circRNA) molecules were recently discovered as a new class of non-coding regulatory RNAs. Read More

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http://doi.wiley.com/10.1111/exd.13890
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http://dx.doi.org/10.1111/exd.13890DOI Listing
January 2019
8 Reads

MiR-744-3p regulates keratinocyte proliferation and differentiation via targeting KLLN in psoriasis.

Exp Dermatol 2019 Jan 21. Epub 2019 Jan 21.

Department of Dermatology, Beijing Friendship Hospital, Capital Medical University, No. 95 Yongan Rd, Beijing100050, China.

Psoriasis is a chronic inflammatory disease and microRNAs have been reported to regulate the pathogenesis of psoriasis. Up-regulated miR-744-3p level was identified to associate with psoriasis while the precise functions of miR-744-3p in psoriasis were not well-elucidated. We first confirmed the up-regulation of miR-744-3p in psoriasis by measuring the its expression level in psoriatic samples. Read More

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http://dx.doi.org/10.1111/exd.13888DOI Listing
January 2019
2 Reads

Anagen hair follicle repair: timely regenerative attempts from plastic extra-bulge epithelial cells.

Exp Dermatol 2019 Jan 21. Epub 2019 Jan 21.

Department of Biomedical Engineering, National Taiwan University, Taipei, Taiwan.

Anagen hair follicle repair is the regenerative scheme activated to restore the structure and hair growth following injuries to anagen hair follicles. Compared with telogen-to-anagen regeneration and hair follicle neogenesis, anagen hair follicle repair is a clinically important, yet relatively unexplored regenerative feature of hair follicles. Due to their highly proliferative character, germinative cells and matrix cells within hair bulbs are highly susceptible to injuries, such as chemotherapy and radiotherapy. Read More

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http://dx.doi.org/10.1111/exd.13889DOI Listing
January 2019
1 Read

Are melanocortin peptides future therapeutics for cutaneous wound healing?

Exp Dermatol 2019 Jan 20. Epub 2019 Jan 20.

Department of Dermatology, Laboratory for Neuroendocrinology of the Skin and Interdisciplinary Endocrinology, University of Münster, Münster, Germany.

Cutaneous wound healing is a complex process divided into different phases, that is an inflammatory, proliferative and remodelling phase. During these phases, a variety of resident skin cell types but also cells of the immune system orchestrate the healing process. In the last year, it has been shown that the majority of cutaneous cell types express the melanocortin 1 receptor (MC1R) that binds α-melanocyte-stimulating hormone (α-MSH) with high affinity and elicits pleiotropic biological effects, for example modulation of inflammation and immune responses, cytoprotection, antioxidative defense and collagen turnover. Read More

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http://doi.wiley.com/10.1111/exd.13887
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http://dx.doi.org/10.1111/exd.13887DOI Listing
January 2019
9 Reads

Replicative senescence of human dermal fibroblasts affects structural and functional aspects of the golgi apparatus.

Exp Dermatol 2019 Jan 19. Epub 2019 Jan 19.

Laboratoire Glycobiologie et Matrice Extracellulaire Végétale, Normandie Université, UNIROUEN, Fédération de recherche Normandie-Végétal - FED 4277, GDR CNRS 3711 COSM'ACTIFS, Mont-Saint-Aignan, France.

It is well recognized that the world population is aging rapidly. Therefore, it is important to understand aging processes at the cellular and molecular levels to predict the onset of age-related diseases and prevent them. Recent research has focused on the identification of aging biomarkers, including those associated with the properties of the Golgi apparatus. Read More

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http://dx.doi.org/10.1111/exd.13886DOI Listing
January 2019
2 Reads

Bacterial biofilm is associated with higher levels of regulatory T-cells in unaffected hidradenitis suppurativa skin.

Exp Dermatol 2019 Jan 18. Epub 2019 Jan 18.

Departments of Dermatology University Hospital Zealand, Roskilde.

Background: The role of bacterial biofilm in hidradenitis suppurativa (HS) is highly debated. Less biofilm is found in clinically unaffected axillary perilesional skin of HS patients compared with healthy controls.

Objective: To study the correlation between biofilm and the phenotypical characterization of the pre-clinical inflammatory infiltrate. Read More

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http://dx.doi.org/10.1111/exd.13885DOI Listing
January 2019
1 Read

Tropisetron via α7 nicotinic acetylcholine receptor suppresses tumor necrosis factor-α-mediated cell responses of human keratinocytes.

Exp Dermatol 2019 Jan 17. Epub 2019 Jan 17.

Department of Dermatology, University of Münster, Münster, Germany.

Tropisetron is a serotonin receptor (5-HT-R)-modulating agent and approved as an antiemetic for patients undergoing chemotherapy. In the gut, it acts via specific serotonin receptors, 5-HT -R, to elicit its beneficial effects against nausea. We investigated whether tropisetron can affect inflammatory cell responses of human primary epidermal keratinocytes (NHK) which are key cells in the regulation of skin homoeostasis. Read More

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http://dx.doi.org/10.1111/exd.13883DOI Listing
January 2019
1 Read

Exploring the potential for rosacea therapeutics of siRNA dispersion in topical emulsions.

Exp Dermatol 2019 Jan 16. Epub 2019 Jan 16.

Umbed Pharmaceuticals, Frederiksberg, Denmark.

Rosacea is a prevalent skin condition dependent on the individual genetic profile. The current pharmacological management of this condition is mostly based on small molecule drugs predominately effective in ameliorating the inflammatory condition. Emerging molecular approaches could present an opportunity for managing rosacea conditions at transcriptomic level, and in the future allow personalized approaches. Read More

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http://dx.doi.org/10.1111/exd.13881DOI Listing
January 2019
1 Read

Targeting mTORC1/2 with OSI-027 inhibits proliferation and migration of keloid keratinocytes.

Exp Dermatol 2019 Jan 16. Epub 2019 Jan 16.

Department of Dermatology and Dermatologic Surgery, Shanghai Ninth People's Hospital, Affiliated to Shanghai Jiaotong University School of Medicine, Center for Specialty Strategy Research of Shanghai, JiaoTong University China Hospital Development Institute, Shanghai, China.

Keloid is a dermal proliferative disorder characterized by the excessive proliferation and migration of keratinocytes and fibroblasts. Over-activation of the serine/threonine protein kinase, mammalian target of rapamycin (mTOR), plays a pivotal role in the process. Here, we show that both mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2) were hyper-activated in keloid-derived primary keratinocytes. Read More

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http://dx.doi.org/10.1111/exd.13882DOI Listing
January 2019
6 Reads

Clinical Snippets.

Authors:

Exp Dermatol 2019 Jan;28(1)

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http://dx.doi.org/10.1111/exd.13866DOI Listing
January 2019
1 Read

Proteomic analysis of stratum corneum in Cutaneous T-Cell Lymphomas and psoriasis.

Exp Dermatol 2019 Jan 13. Epub 2019 Jan 13.

Galderma R&D, Les Templiers, Sophia-Antipolis, France.

Stratum corneum collected by tape stripping from 10 and 24 subjects with cutaneous T-cell lymphomas (CTCL) or psoriasis, respectively, were compared using quantitative label-free mass spectrometry analysis. A non-supervised statistical analysis (Posneg NMF) based on 352 differentially expressed proteins in both CTCL and psoriasis samples was able to separate the two disease groups and finally able to identify a set of 112 proteins that contributed most and significantly to the separation when compared to non-lesional samples. In addition, Luminex assay revealed that the increase in the amount of chemokines related to the inflammatory response, and immune cell infiltration and recruitment in lesional stratum corneum in CTCL, including CXCL8, CXCL9, CXCL10, CCL27, TNF and sICAM-1 was in agreement with published data on entire skin biopsies. Read More

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http://dx.doi.org/10.1111/exd.13880DOI Listing
January 2019
1 Read

Melanoma types by in vivo reflectance confocal microscopy correlated with protein and molecular genetic alterations: A pilot study.

Exp Dermatol 2019 Jan 12. Epub 2019 Jan 12.

Department of Surgery, Medicine, Dentistry and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Modena, Italy.

Cutaneous melanoma (CM) is one of the most prevalent skin cancers, which lacks both a prognostic marker and a specific and lasting treatment, due to the complexity of the disease and heterogeneity of patients. Reflectance confocal microscopy (RCM) in vivo analysis is a versatile approach offering immediate morphological information, enabling the identification of four primary cutaneous RCM CM types. Whether RCM CM types are associated with a specific protein and molecular genetic profiles at the tissue level remains unclear. Read More

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http://dx.doi.org/10.1111/exd.13877DOI Listing
January 2019
5 Reads

Vitiligo as a skin memory disease: The need for early intervention with immunomodulating agents and a maintenance therapy to target resident memory T cells.

Exp Dermatol 2019 Jan 12. Epub 2019 Jan 12.

INSERM U 1035, BMGIC, Immuno-Dermatology, ATIP-AVENIR, Bordeaux University, Bordeaux, France.

The understanding of the immune mechanisms of vitiligo has profoundly improved over the past years. The recent discovery of a new population of antigen-experienced memory T cells called resident memory T cells (T ) has changed the concept of immune surveillance in peripheral tissue as skin, and the presence of melanocyte-specific T is clearly demonstrated in vitiligo, a disease that could be now seen such as a memory skin disease. This review summarizes the recent knowledge on skin T and their role in vitiligo. Read More

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http://doi.wiley.com/10.1111/exd.13879
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http://dx.doi.org/10.1111/exd.13879DOI Listing
January 2019
7 Reads

1-(2-Hydroxyethyl)-2-imidazolidinone, a heparanase and matrix metalloproteinase inhibitor, improves epidermal basement membrane structure and epidermal barrier function.

Exp Dermatol 2019 Jan 12. Epub 2019 Jan 12.

Shiseido Global Innovation Center.

Daily exposure to sunlight is known to affect the structure and function of the epidermal basement membrane (BM), as well as epidermal differentiation and epidermal barrier function. The aim of this study is to clarify whether the inhibition of BM-degrading enzymes such as heparanase and matrix metalloproteinase 9 (MMP-9) can improve the epidermal barrier function of facial skin, which is exposed to the sun on a daily basis. 1-(2-hydroxyethyl)-2-imidazolidinone (HEI) was synthesized as an inhibitor of both heparanase and MMP-9. Read More

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http://dx.doi.org/10.1111/exd.13876DOI Listing
January 2019
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C-reactive protein (CRP) rs3093059C predicts poor mizolastine response in chronic spontaneous urticaria patients with elevated serum CRP level.

Exp Dermatol 2019 Jan 11. Epub 2019 Jan 11.

Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China.

Chronic spontaneous urticaria (CSU) is a frequent disorder with recurrent itchy wheals and/or angioedema, and nearly 35% patients respond poorly to non-sedating H1 antihistamine treatment. CRP gene encodes the C-reactive protein, which is involved in the pathogenesis of CSU. To investigate the impacts of CRP polymorphisms on the susceptibility and therapeutic efficacy in the South Han CSU patients, we enrolled 145 CSU patients in our study. Read More

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http://doi.wiley.com/10.1111/exd.13874
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http://dx.doi.org/10.1111/exd.13874DOI Listing
January 2019
6 Reads
3.762 Impact Factor

Ageing attenuates muscarinic-mediated sweating differently in men and women with no effect on nicotinic-mediated sweating.

Exp Dermatol 2019 Jan 10. Epub 2019 Jan 10.

Human and Environmental Physiology Research Unit, University of Ottawa, Ottawa, Ontario, Canada.

Ageing attenuates muscarinic-mediated sweating. However, whether ageing also impairs nicotinic-mediated sweating remains unclear. Further, despite the known sex-related differences in peripheral sweat gland function, it remains unclear whether age-related modifications of muscarinic and nicotinic-mediated sweating, if any, are similar between men and women. Read More

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http://dx.doi.org/10.1111/exd.13878DOI Listing
January 2019
1 Read

Regeneration of rete ridges in Lanyu pig (Sus scrofa): Insights for human skin wound healing.

Exp Dermatol 2019 Jan 10. Epub 2019 Jan 10.

International Center for Wound Repair and Regeneration, National Cheng Kung University, Tainan, TW.

Rete ridges are important to the mechanical function of skin in animals with minimal hair, including humans. As mice do not exhibit rete ridges, the need for a quality animal model is pertinent. Here, we develop a Lanyu pig (Sus scrofa) full-thickness wound model to explore tissue regeneration because the architecture and function is similar to humans and inbred genetic variants are available. Read More

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http://doi.wiley.com/10.1111/exd.13875
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http://dx.doi.org/10.1111/exd.13875DOI Listing
January 2019
5 Reads
3.762 Impact Factor

Vitamin D modulates the allergic phenotype of dendritic cells in children with atopic dermatitis.

Exp Dermatol 2019 Jan 9. Epub 2019 Jan 9.

Department of Pediatric Infectious Diseases and Immunology, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.

Vitamin D (VD) deficiency has been associated with increased incidence and severity of atopic dermatitis (AD), but the mechanisms through which VD may ameliorate AD are unclear. We compared the phenotypic characteristics of circulating myeloid and plasmacytoid dendritic cells (mDCs and pDCs, respectively) of children with AD vs healthy controls (HC) and evaluated if VD can modulate the allergic phenotype of circulating DCs in AD patients. Although there was no difference in frequency of circulating DCs between groups, among children with AD there was an inverse correlation between SCORAD and circulating total DCs and mDCs. Read More

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http://doi.wiley.com/10.1111/exd.13873
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http://dx.doi.org/10.1111/exd.13873DOI Listing
January 2019
7 Reads
3.762 Impact Factor

Epigenetic control in skin development, homeostasis and injury repair.

Exp Dermatol 2019 Jan 9. Epub 2019 Jan 9.

Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York.

Cell-type- and cell-state-specific patterns of covalent modifications on DNA and histone tails form global epigenetic profiles that enable spatiotemporal regulation of gene expression. These epigenetic profiles arise from coordinated activities of transcription factors and epigenetic modifiers, which result in cell-type-specific outputs in response to dynamic environmental conditions and signalling pathways. Recent mouse genetic and functional studies have highlighted the physiological significance of global DNA and histone epigenetic modifications in skin. Read More

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http://dx.doi.org/10.1111/exd.13872DOI Listing
January 2019
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IL-17A synergistically enhances TLR3-mediated IL-36γ production by keratinocytes: A potential role in injury-amplified psoriatic inflammation.

Exp Dermatol 2019 Jan 7. Epub 2019 Jan 7.

Department of Dermatology, Shanghai Skin Disease Hospital, Shanghai, China.

Skin injury can trigger formation of new lesions in psoriasis (Koebner phenomenon). The mechanisms through which injury exacerbates psoriasis are unclear. During wound repair, epidermal keratinocytes are activated and produce abundant IL-36γ, further promoting the skin inflammation. Read More

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http://doi.wiley.com/10.1111/exd.13871
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http://dx.doi.org/10.1111/exd.13871DOI Listing
January 2019
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Single cell analyses reveal specific distribution of anti-bacterial molecule Perforin-2 in human skin and its modulation by wounding and Staphylococcus aureus infection.

Exp Dermatol 2019 Jan 4. Epub 2019 Jan 4.

Department of Dermatology and Cutaneous Surgery, Wound Healing and Regenerative Medicine Research Program, University of Miami Miller School of Medicine, Miami, Florida.

Perforin-2 (P-2) is a recently described antimicrobial protein with unique properties to kill intracellular bacteria. We investigated P-2 expression pattern and cellular distribution in human skin and its importance in restoration of barrier function during wound healing process and infection with the common wound pathogen Staphylococcus aureus. We describe a novel approach for the measurement of P-2 mRNA within individual skin cells using an amplified fluorescence in situ hybridization (FISH) technique. Read More

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http://dx.doi.org/10.1111/exd.13870DOI Listing
January 2019
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Mechanistic insight into the activity of a sulfone compound dapsone on Propionibacterium (Newly Reclassified as Cutibacterium) Acnes-mediated cytokine production.

Exp Dermatol 2019 Feb;28(2):190-197

Allergan plc, Irvine, California.

Acne remains as the most common skin disease in the United States even despite multiple approved topical and systemic medications available. These treatments available over the counter and by prescription can be classified based on comedolytic, antibacterial and anti-inflammatory activities and are often used in combination. Therefore, understanding of the mechanism of action is critical to achieving the best clinical outcome and synergy. Read More

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http://doi.wiley.com/10.1111/exd.13869
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http://dx.doi.org/10.1111/exd.13869DOI Listing
February 2019
8 Reads