21,885 results match your criteria Experimental Cell Research [Journal]


Overexpression of microRNA-204-5p alleviates renal ischemia-reperfusion injury in mice through blockage of Fas/FasL pathway.

Exp Cell Res 2019 Apr 19. Epub 2019 Apr 19.

Department of Kidney Medicine, Linyi City People's Hospital, No. 27, Eastern Section of Jiefang Road, Linyi, 276000, PR China. Electronic address:

The multiple roles of microRNA-204-5p (miR-204-5p) in numerous types of cancer have been reported, but its function in renal ischemia-reperfusion injury (RIRI) remains unclear. In this study, we aim to explore whether miR-204-5p was implicated in the RIRI in mice via regulating the Fas/Fas ligand (FasL) pathway. Firstly, the Gene Expression Omnibus (GEO) database was used to screen RIRI-related differentially expressed genes (DEGs). Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.04.023DOI Listing

LncRNA FOXD2-AS1 plays an oncogenic role in hepatocellular carcinoma through epigenetically silencing CDKN1B(p27) via EZH2.

Exp Cell Res 2019 Apr 17. Epub 2019 Apr 17.

Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery First Affiliated Hospital, School of Medicine, Zhejiang University, PR China; NHFPC Key Laboratory of Combined Multi-organ Transplantation, PR China; Key Laboratory of the Diagnosis and Treatment of Organ Transplantation, Chinese Academy of Medical Sciences, PR China; Key Laboratory of Organ Transplantation, Zhejiang Province, Hangzhou, PR China; Collaborative Innovation Center for Diagnosis Treatment of Infectious Diseases, Hangzhou, Zhejiang, 310003, PR China. Electronic address:

Accumulating reports suggest that long noncoding RNAs (lncRNAs) play critical roles in the progression of many tumors. In this study, we explored the expression level of lncRNA FOXD2-AS1 in the tumorigenesis of hepatocellular carcinoma (HCC). The data indicated that FOXD2-AS1 expression was increased in HCC specimens and cell lines. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.04.016DOI Listing
April 2019
3.246 Impact Factor

Differing calcification processes in cultured vascular smooth muscle cells and osteoblasts.

Exp Cell Res 2019 Apr 17. Epub 2019 Apr 17.

Department of Comparative Biomedical Sciences, Royal Veterinary College, London, UK. Electronic address:

Arterial medial calcification (AMC) is the deposition of calcium phosphate mineral, often as hydroxyapatite, in the medial layer of the arteries. AMC shares some similarities to skeletal mineralisation and has been associated with the transdifferentiation of vascular smooth muscle cells (VSMCs) towards an osteoblast-like phenotype. This study used primary mouse VSMCs and calvarial osteoblasts to directly compare the established and widely used in vitro models of AMC and bone formation. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144827193019
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http://dx.doi.org/10.1016/j.yexcr.2019.04.020DOI Listing
April 2019
4 Reads

Hypoxia alters splicing of the cancer associated Fas gene.

Exp Cell Res 2019 Apr 17. Epub 2019 Apr 17.

Department of Immunology and Cell Biology, Vilnius University, Institute of Biotechnology, Vilnius, Lithuania. Electronic address:

The removal of introns from mRNA precursors (pre-mRNAs) is an essential step in eukaryotic gene expression. The splicing machinery heavily contributes to biological complexity and especially to the ability of cells to adapt to altered cellular conditions. Hypoxia also plays a key role in the pathophysiology of many disease states. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.04.015DOI Listing

CSTP1 inhibits IL-6 expression through targeting Akt/FoxO3a signaling pathway in bladder cancer cells.

Exp Cell Res 2019 Apr 16. Epub 2019 Apr 16.

Department of Gastrointestinal Surgery, Qingyuan People's Hospital, The Six Affiliated Hosptial of Guangzhou Medical University, Qingyuan City, 511518, China. Electronic address:

CSTP1, a recently identified protein phosphotase, is frequently repressed in bladder cancers. Previous results showed that CSTP1 over-expression inhibited cell cycle progression and promoted apoptosis through dephosphorylating Akt kinase at Ser473 site in bladder cancer cells, but the mechanisms how CSTP1 exerted tumor suppressive activity remains unclear. In this study, we analyzed the gene expression profile changes that affected by CSTP1 overexpression by microarray, and reported that CSTP1 decreased IL-6 expression/secretion in bladder cancer cells and re-expression of IL-6 abrogated CSTP1's tumor suppressive activity. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.04.019DOI Listing
April 2019
2 Reads

Human adipocytes and CD34 cells from the stromal vascular fraction of the same adipose tissue differ in their energy metabolic enzyme configuration.

Exp Cell Res 2019 Apr 16. Epub 2019 Apr 16.

Department of Cell Biology, University Medicine Rostock, Schillingallee 69, 18057 Rostock, Germany. Electronic address:

Adipose tissue plays a role in energy storage and metabolic balance and is composed of different cell types. The metabolic activity of the tissue itself has been a matter of research for a long time, but comparative data about the energy metabolism of different cell types of human subcutaneous adipose tissue are sparse. Therefore, we compared the activity of major energy metabolic pathways of adipocytes and CD34 cells from the stromal vascular fraction (SVF) separated from the same tissue. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.04.018DOI Listing
April 2019
2 Reads

Epigallocatechin-3-gallate stimulates autophagy and reduces apoptosis levels in retinal Müller cells under high-glucose conditions.

Exp Cell Res 2019 Apr 15. Epub 2019 Apr 15.

Department of Ophthalmology, Lixiang Eye Hospital of Soochow University, Suzhou, 215021, China. Electronic address:

Retinal neurodegeneration is an early feature in the pathogenesis of diabetic retinopathy (DR). Autophagy is an intracellular catabolic process involved in protein and organelle degradation that has been linked in DR. Epigallocatechin gallate (EGCG) is a major polyphenol in green tea that has beneficial effects in diabetic. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.04.014DOI Listing
April 2019
1 Read

Lipotoxicity reduces β cell survival through islet stellate cell activation regulated by lipid metabolism-related molecules.

Exp Cell Res 2019 Apr 15. Epub 2019 Apr 15.

Department of Endocrinology, Zhongda Hospital, Institute of Diabetes, School of Medicine, Southeast University, Nanjing, China. Electronic address:

Background: Islet stellate cells (ISCs) activation is mainly associated with islet fibrosis, which contributes to the progression of type 2 diabetes. However, the molecular mechanism underlying this process is not fully understood.

Methods: In order to investigate this process the current study examined ectopic fat accumulation in rats with high-fat diet (HFD) induced obesity. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.04.012DOI Listing
April 2019
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Restoring retinal neurovascular health via substance P.

Exp Cell Res 2019 Apr 14. Epub 2019 Apr 14.

Academic Unit of Ophthalmology, Bristol Medical School, University of Bristol, Bristol, UK; National Institute for Health Research (NIHR) Biomedical Research Centre at Moorfields Eye Hospital and University College London Institute of Ophthalmology, London, UK. Electronic address:

Regulation of vascular permeability plays a major role in the pathophysiology of visually threatening conditions such as retinal vein occlusion and diabetic retinopathy. Principally, several factors such as vascular endothelial growth factor (VEGF), are up-regulated or induced in response to hypoxia thus adversely affecting the blood-retinal barrier (BRB), resulting in retinal edema and neovascularisation. Furthermore, current evidence supports a dysregulation of the inner retinal neural-vascular integrity as a critical factor driving retinal ganglion cell (RGC) death and visual loss. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144827193015
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http://dx.doi.org/10.1016/j.yexcr.2019.04.008DOI Listing
April 2019
1 Read

Corrigendum to "p85 beta-PIX is required for cell motility through phosphorylations of focal adhesion kinase and p38 MAP kinase"[Exp. Cell Res. 307(2) (2005) 315-328].

Exp Cell Res 2019 Jun 13;379(2):245-247. Epub 2019 Apr 13.

Department of Pharmacology, College of Medicine, Konyang University, Nonsan, 320-711, Republic of Korea. Electronic address:

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http://dx.doi.org/10.1016/j.yexcr.2019.03.030DOI Listing

Screening of pure synthetic coating substrates for induced pluripotent stem cells and iPSC-derived neuroepithelial progenitors with short peptide based integrin array.

Exp Cell Res 2019 Apr 11. Epub 2019 Apr 11.

Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, Henan, 450052, China; Department of Chemical and Life Science Engineering, Virginia Commonwealth University, Richmond, VA, 23220, USA. Electronic address:

Simple and pure synthetic coating substrates are needed to overcome the disadvantages of traditional coating products like animal derived Matrigel in stem cell research. Since integrins are of great importance in cell adhesion and cell-ECM communication, in this study, a commercially available integrin array established by synthetic integrin binding peptides is used to screen coating substrates for iPSCs and NEPs. The results showed that binding peptides of integrin α5β1, αVβ1, αMβ2 and αIIbβ3 supported cell adhesion of iPSCs, while α5β1, αVβ1 and αIIbβ3 binding peptides supported NEPs adhesion. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144827193018
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http://dx.doi.org/10.1016/j.yexcr.2019.04.013DOI Listing
April 2019
2 Reads

Programming of ES cells and reprogramming of fibroblasts into renal lineage-like cells.

Exp Cell Res 2019 Jun 11;379(2):225-234. Epub 2019 Apr 11.

Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran. Electronic address:

This study aims to prepare intermediate mesoderm-like cells from mouse embryonic fibroblasts (MEFs). In the first step, intermediate mesoderm-like cells (IMLCs) and renal epithelial-like cells (RELCs) were extracted from mouse embryonic stem cells (mESCs) in a specified media that contained two small molecules, CHIR99021 and TTNPB, along with growth factors, FGF9and BMP7. Then, MEFs were directly converted into IM by genes for the pluripotency factors, which encode the transcription factors; Oct4, Sox2, Klf4, and c-Myc (OSKM). Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.04.011DOI Listing

A role for Rab11 in the homeostasis of the endosome-lysosomal pathway.

Exp Cell Res 2019 Apr 11. Epub 2019 Apr 11.

The Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Victoria, 3010, Australia. Electronic address:

The small GTPases Rab11a and 11b are key regulators of membrane transport, localised to the recycling endosomes and also early endosomes. The function of Rab11 within the recycling pathway has been well defined, however, the role of Rab11 at the early endosomes remains poorly characterised. Here, we have generated HeLa cell lines devoid of either Rab11a or Rab11b using CRISPR/Cas9 to functionally dissect the roles of these two Rab11 family members in recycling and in the endosomal-lysosomal system. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.04.010DOI Listing

In vivo characteristics of human and mouse breast tumor cell lines.

Exp Cell Res 2019 Apr 10. Epub 2019 Apr 10.

Department of Respiratory Medicine, Laboratory of Translational Immunology, University Medical Center Utrecht, the Netherlands. Electronic address:

Although two- and three-dimensional in vitro studies of breast tumor cell lines have increased our knowledge on tumor growth and metastasis formation, the complex in vivo microenvironment is not taken into consideration. The goal of our study was to illustrate the in vivo morphology and motility of widely used breast tumor cell lines. Intravital microscopy allows real-time visualization of individual cells inside tissues of living animals. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.04.009DOI Listing

Exosomal miR-499a-5p promotes cell proliferation, migration and EMT via mTOR signaling pathway in lung adenocarcinoma.

Exp Cell Res 2019 Jun 10;379(2):203-213. Epub 2019 Apr 10.

Shanghai Lung Cancer Center, Shanghai Chest Hospital, Shanghai Jiao Tong University, West Huaihai Road 241, Shanghai, 200030, China. Electronic address:

Tumor-derived exosomes contain informative microRNAs involved in carcinogenesis, cell migration, invasion and epithelial-mesenchymal transition (EMT), eventually contributing to metastasis of cancers. This study aims to clarify which and how exosomal miRNA affects tumor carcinogenesis and metastasis. Among them, miR-499a-5p was upregulated in both highly metastatic lung cancer cell line and their exosomes. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.035DOI Listing

KDM5B promotes breast cancer cell proliferation and migration via AMPK-mediated lipid metabolism reprogramming.

Exp Cell Res 2019 Jun 10;379(2):182-190. Epub 2019 Apr 10.

College of Life Science & Bioengineering, Beijing University of Technology, Pingleyuan 100(#), District of Chaoyang, Beijing, 100124, China.

Lysine demethylase 5B (KDM5B) is up-regulated in many cancers, including breast cancer. However, the underlying metabolic mechanisms of KDM5B on breast cancer progression are poorly understood. Here, we showed that KDM5B expression positively correlates with metastasis in breast cancer. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144827193015
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http://dx.doi.org/10.1016/j.yexcr.2019.04.006DOI Listing
June 2019
10 Reads

Hypoxia-induced disruption of neural vascular barrier is mediated by the intracellular induction of Fe(II) ion.

Exp Cell Res 2019 Jun 7;379(2):166-171. Epub 2019 Apr 7.

Department of Pathology, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi, 755-8505, Japan. Electronic address:

Neural vascular barrier maintains the optimal tissue microenvironment of central nervous system in which neural cells can function normally. In various neural diseases, the decrease in oxygen concentration, hypoxia, of affected tissues is known to accelerate the disease progression through disruption of neural vascular barrier. Therefore, the clarification of mechanisms underlying hypoxia-induced disruption of neural vascular barrier would definitely lead to the establishment of new effective therapies for intractable neural diseases. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.04.003DOI Listing

Dynamin inhibitors impair platelet-derived growth factor β-receptor dimerization and signaling.

Exp Cell Res 2019 Apr 7. Epub 2019 Apr 7.

Department of Pharmaceutical Biosciences, Uppsala University, Uppsala, Sweden.

The role of plasma membrane composition and dynamics in the activation process of receptor tyrosine kinases (RTKs) is still poorly understood. In this study we have investigated how signaling via the RTK, platelet-derived growth factor β-receptor (PDGFR-β) is affected by Dynasore or Dyngo-4a, which are commonly used dynamin inhibitors. PDGFR-β preferentially internalizes via clathrin-coated pits and in this pathway, Dynamin II has a major role in the formation and release of vesicles from the plasma membrane by performing the membrane scission. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.04.004DOI Listing

PACS2 is required for ox-LDL-induced endothelial cell apoptosis by regulating mitochondria-associated ER membrane formation and mitochondrial Ca elevation.

Exp Cell Res 2019 Jun 7;379(2):191-202. Epub 2019 Apr 7.

Institute of Cardiovascular Diseases, Xinqiao Hospital, Army Medical University (Third Military Medical University), Chongqing, China. Electronic address:

Oxidized low-density lipoprotein (ox-LDL)-induced endothelial cell (EC) apoptosis is the initial step of atherogenesis and associated with Ca overload. Mitochondria-associated endoplasmic reticulum (ER) membrane (MAM), regulated by tethering proteins such as phosphofurin acidic cluster sorting protein 2 (PACS2), is essential for mitochondrial Ca overload by mediating ER-mitochondria Ca transfer. In our study, we aimed to investigate the role of PACS2 in ox-LDL-induced apoptosis in human umbilical vein endothelial cells (HUVECs) and the underlying mechanisms. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.04.002DOI Listing

miR-629-5p promotes growth and metastasis of hepatocellular carcinoma by activating β-catenin.

Exp Cell Res 2019 Apr 4. Epub 2019 Apr 4.

Department of Infectious Disease, Heping Hospital Attached to Changzhi Medical College, Changzhi, 046000, China. Electronic address:

Aberrant miR-629-5p expression in several cancer types has been reported. Nonetheless, its potential effect and mechanism of action on tumor growth and metastasis in hepatocellular carcinoma (HCC) have rarely been analyzed. In this study, we found that miR-629-5p was upregulated in HCC tissue samples as compared to matched adjacent-tissue samples. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.042DOI Listing
April 2019
3 Reads

Neuron-specific knockdown of Drosophila HADHB induces a shortened lifespan, deficient locomotive ability, abnormal motor neuron terminal morphology and learning disability.

Exp Cell Res 2019 Jun 3;379(2):150-158. Epub 2019 Apr 3.

Department of Applied Biology, Advanced Insect Research Promotion Center, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan. Electronic address:

Mutations in the HADHB gene induce dysfunctions in the beta-oxidation of fatty acids and result in a MTP deficiency, which is characterized by clinical heterogeneity, such as cardiomyopathy and recurrent Leigh-like encephalopathy. In contrast, milder forms of HADHB mutations cause the later onset of progressive axonal peripheral neuropathy (approximately 50-80%) and myopathy with or without episodic myoglobinuria. The mechanisms linking neuronal defects in these diseases to the loss of HADHB function currently remain unclear. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.040DOI Listing
June 2019
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The cyanobacterial neurotoxin β-N-methylamino-l-alanine prevents addition of heparan sulfate to glypican-1 and increases processing of amyloid precursor protein in dividing neuronal cells.

Exp Cell Res 2019 Jun 3;379(2):172-181. Epub 2019 Apr 3.

Department of Experimental Medical Science, Division of Neuroscience, Glycobiology Group, Lund University, Biomedical Center A13, SE-221 84, Lund, Sweden. Electronic address:

The neurotoxin β-N-methylamino-l-alanine replaces l-serine in proteins and produces Alzheimer-like pathology. In proteoglycans, e.g. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.041DOI Listing
June 2019
2 Reads

IL-10 in vitro could enhance IFNγ expression and suppress PD-1 expression in CD8 T cells from esophageal cancer patients.

Authors:
Chong Li Wenwei Zuo

Exp Cell Res 2019 Jun 2;379(2):159-165. Epub 2019 Apr 2.

Department of Oncology and Southwest Cancer Center, Southwest Hospital, The Third Military Medical University (Army Medical University), Chongqing, China. Electronic address:

IL-10 is commonly regarded as an immunoregulatory cytokine, but accumulating evidence suggests that IL-10 may promote CD8 T cell expansion and proliferation. In this study, tumor infiltrating (TI) and peripheral blood (PB) CD8 T cells were collected from esophageal cancer patients. Interestingly, IL-10 concentration in the tumor microenvironment increased with advancing tumor stage, while TI CD8 T cell-mediated IL-10 production decreased with advancing tumor stage. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.038DOI Listing

Changes in the NF-κB signaling pathway in juvenile and adult patients with Kashin-Beck disease.

Exp Cell Res 2019 Jun 2;379(2):140-149. Epub 2019 Apr 2.

School of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an, Shaanxi 710061, PR China. Electronic address:

To investigate the pathogenesis of Kashin-Beck disease (KBD), we compared the common signaling pathways in peripheral blood mononuclear cells (PBMCs) obtained from healthy juvenile and adults and KBD patients, and also from osteoarthritis (OA) patients. The PBMCs from 12 KBD and 12 healthy juvenile, and those from 20 adult KBD patients and 12 healthy donors were separately collected among the people living in the KBD endemic area. The patients were distinguished according to the national diagnosis criteria. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.04.001DOI Listing
June 2019
2 Reads
3.246 Impact Factor

Distinctive role of dysregulated miRNAs in chordoma cancer stem-like cell maintenance.

Exp Cell Res 2019 Apr 2. Epub 2019 Apr 2.

Department of Medical Genetics, Yeditepe University Medical School, 34755, Istanbul, Turkey. Electronic address:

Chordoma is a rare, slow-growing tumor thought to arise from remnants of embryonic notochord associated with an aggressive outcome. Cancer stem-like cells (CSCs) are related to tumorigenesis, recurrence, and resistance in cancers. Therefore, chordoma CSCs are possible targets for chordoma treatment. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.039DOI Listing
April 2019
3.246 Impact Factor

The inner life of integrin adhesion sites: From single molecules to functional macromolecular complexes.

Exp Cell Res 2019 Jun 31;379(2):235-244. Epub 2019 Mar 31.

Univ. Bordeaux, Interdisciplinary Institute for Neuroscience, UMR5297, 33000, Bordeaux, France; CNRS, Interdisciplinary Institute for Neuroscience, UMR5297, 33000, Bordeaux, France. Electronic address:

Cells are mechanical living machines that remodel their microenvironment by adhering and generating forces on the extracellular matrix (ECM) using integrin-dependent adhesion sites (IAS). In return, the biochemical and physical nature of the ECM determines cellular behavior and morphology during proliferation, differentiation and migration. IAS come in different shapes and forms. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.036DOI Listing
June 2019
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Deciphering the galectin-12 protein interactome reveals a major impact of galectin-12 on glutamine anaplerosis in colon cancer cells.

Exp Cell Res 2019 Jun 29;379(2):129-139. Epub 2019 Mar 29.

Department of Applied Tumor Biology, Institute of Pathology, University Hospital Heidelberg, Germany; Clinical Cooperation Unit Applied Tumor Biology, German Cancer Research Center (DKFZ), Heidelberg, Germany. Electronic address:

Galectins are β-galactoside binding proteins which possess a variety of functions including modulation of apoptosis, growth and differentiation. Hence, alterations in the expression profile have been associated with loss of cellular homeostasis contributing to tumor growth and progression. Though galectin-12 is significantly downregulated in several tumor entities, including colon cancer, its impact on cellular homeostasis as well as galectin-12 specific binding partners have not been identified so far. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.032DOI Listing
June 2019
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Identification of stemness in primary retinoblastoma cells by analysis of stem-cell phenotypes and tumorigenicity with culture and xenograft models.

Exp Cell Res 2019 Jun 30;379(1):110-118. Epub 2019 Mar 30.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, China. Electronic address:

Retinoblastoma (RB) is a primary intraocular malignancy in childhood, and may develop relapse and metastatic disease. This study was to identify the stem-cell properties of primary retinoblastoma cells critical to tumorigenesis and metastasis. Primary cells were isolated from fresh human RB tissues after enucleation, and cultured in serum-free or serum-enriched conditions, with two RB cell-lines Weri-RB1 and Y79 for comparison. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144827193013
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http://dx.doi.org/10.1016/j.yexcr.2019.03.034DOI Listing
June 2019
10 Reads

MicroRNA-451 inhibits vascular smooth muscle cell migration and intimal hyperplasia after vascular injury via Ywhaz/p38 MAPK pathway.

Exp Cell Res 2019 Jun 29;379(2):214-224. Epub 2019 Mar 29.

Department of Interventional Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China.

Increasing evidence has indicated that intimal hyperplasia is a common event in the pathophysiology of many vascular diseases including atherosclerosis (AS). Recently, deregulated microRNAs (miRNAs) have been reported to be associated with the pathophysiology of AS. However, the biological function and regulatory mechanisms of miRNAs in intimal hyperplasia in AS remain largely unclear. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.033DOI Listing
June 2019
1 Read

Metabolic switching in pluripotent stem cells reorganizes energy metabolism and subcellular organelles.

Exp Cell Res 2019 Jun 25;379(1):55-64. Epub 2019 Mar 25.

Department of Hematology, St. Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis, TN, 38105, USA. Electronic address:

Metabolic studies of human pluripotent stem cells (hPSCs) have focused on how the cells produce energy through the catabolic pathway. The less-studied anabolic pathway, by which hPSCs expend energy in the form of adenosine triphosphate (ATP), is not yet fully understood. Compared to fully differentiated somatic cells, hPSCs undergo significant changes not only in their gene expression but also in their production and/or expenditure of ATP. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.028DOI Listing
June 2019
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A tumorsphere model of glioblastoma multiforme with intratumoral heterogeneity for quantitative analysis of cellular migration and drug response.

Exp Cell Res 2019 Jun 25;379(1):73-82. Epub 2019 Mar 25.

Laboratory of Immunology and Cancer Biology, Aalborg University, Aalborg, Denmark. Electronic address:

Glioblastoma multiforme (GBM) is the most common and malignant type of primary brain tumor and is characterized by its sudden onset and invasive growth into the brain parenchyma. The invasive tumor cells evade conventional treatments and are thought to be responsible for the ubiquitous tumor regrowth. Understanding the behavior of these invasive tumor cells and their response to therapeutic agents could help improve patient outcome. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.031DOI Listing

Long noncoding RNA CCAT2 promotes hepatocellular carcinoma proliferation and metastasis through up-regulation of NDRG1.

Exp Cell Res 2019 Jun 25;379(1):19-29. Epub 2019 Mar 25.

Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, Department of Infectious Diseases, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China. Electronic address:

Background: Emerging studies demonstrate that long noncoding RNAs (lncRNAs) play crucial roles in hepatocarcinogenesis through various mechanisms. LncRNA CCAT2 was a newly discovered lncRNA and amplified in several cancers. However, the mechanisms involved in function of CCAT2 in hepatocellular carcinoma (HCC) remain to be explored. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.029DOI Listing

Mechanical regulation of myofibroblast phenoconversion and collagen contraction.

Exp Cell Res 2019 Jun 22;379(1):119-128. Epub 2019 Mar 22.

Faculty of Dentistry, University of Toronto, Toronto, ON, M5G 1G6, Canada. Electronic address:

Activated fibroblasts promote physiological wound repair following tissue injury. However, dysregulation of fibroblast activation contributes to the development of fibrosis by enhanced production and contraction of collagen-rich extracellular matrix. At the peak of their activities, fibroblasts undergo phenotypic conversion into highly contractile myofibroblasts by developing muscle-like features, including formation of contractile actin-myosin bundles. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.027DOI Listing

USP28 regulates deubiquitination of histone H2A and cell proliferation.

Exp Cell Res 2019 Jun 22;379(1):11-18. Epub 2019 Mar 22.

Beijing Key Laboratory of Protein Posttranslational Modifications and Cell Function, Peking University Health Science Center, 38 Xueyuan Road, Beijing, 100191, PR China. Electronic address:

Post-translational modifications of the histone H2A represent an important mechanism by which cells modulate the structure and function of chromatin. Ubiquitination at K119 of histone H2A is associated transcriptional repression, which is shown to be regulated by deubiquitinases (DUBs). Here, we performed a screen to identify novel DUBs for histone H2A. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.026DOI Listing
June 2019
3.246 Impact Factor

miR3609 sensitizes breast cancer cells to adriamycin by blocking the programmed death-ligand 1 immune checkpoint.

Exp Cell Res 2019 Mar 20. Epub 2019 Mar 20.

Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Sarcoma Biology Laboratory, UCLA Orthopaedic Surgery, Los Angeles, CA, 90095, USA. Electronic address:

Chemoresistance is the leading cause of breast cancer therapy failure, and studies of the mechanisms underlying chemoresistance and the treatment of drug-resistant tumors remain challenging. In the present study, we discovered a novel microRNA, miR3609, that influences the malignancy of breast cancer. Our results showed miR-3609 expression was lower in resistant breast cancer cells than in sensitive breast cancer cells (MCF-7), while PD-L1 expression was higher in resistant breast cancer cells than in sensitive breast cancer cells. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.025DOI Listing

SPAG5 contributes to the progression of gastric cancer by upregulation of Survivin depend on activating the wnt/β-catenin pathway.

Exp Cell Res 2019 Jun 21;379(1):83-91. Epub 2019 Mar 21.

Department of General Surgery, Suqian First Hospital, Suqian, China. Electronic address:

The sperm-associated antigen 5 (SPAG5) plays a key role in controlling cellular processes, including cell cycle progression and proliferation. However, the role of SPAG5 in gastric cancer (GC) remains unclear. Herein, our study showed that upregulation of SPAG5 was detected frequently in GC tissues, and was associated with significantly worse survival in patients with GC. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.024DOI Listing
June 2019
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Stretching cells - An approach for early cancer diagnosis.

Exp Cell Res 2019 May 19;378(2):191-197. Epub 2019 Mar 19.

Queensland Micro- and Nanotechnology Centre, Nathan Campus, Griffith University, 170 Kessels Road, Brisbane, QLD, 4111, Australia. Electronic address:

Cells express multiple biophysical cues during migration, differentiation, and transformation. Probing and quantifying these biophysical cues could serve as a diagnostic tool for differentiating healthy with neoplastic cells. These biophysical cues may be utilized for diagnostic screening in cancer, as the tumor cells interact with the surrounding extracellular matrix (ECM). Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.01.029DOI Listing

The coordination between ZNF217 and LSD1 contributes to hepatocellular carcinoma progress and is negatively regulated by miR-101.

Exp Cell Res 2019 Jun 18;379(1):1-10. Epub 2019 Mar 18.

Department of Laboratory Medicine, Department of Biochemistry and Molecular Biology, Peking University Third Hospital, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.

Zinc-figure protein 217 (ZNF217) is a C2H2 zinc finger transcription factor that works as a pivotal effector stimulating embryonic immortalization as well as oncogenicity during multiple cancer processes. Nevertheless, the expression of ZNF217 in hepatocellular carcinoma (HCC) and the underlying specific molecular mechanisms remains elusive. In the present study, we aimed to explore the potential role of ZNF217 in HCC cell proliferation and invasion, as well as the underlying mechanism. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.017DOI Listing
June 2019
4 Reads
3.246 Impact Factor

Lysophosphatidic acid guides the homing of transplanted olfactory ensheathing cells to the lesion site after spinal cord injury in rats.

Exp Cell Res 2019 Jun 18;379(1):65-72. Epub 2019 Mar 18.

Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou 215006, China. Electronic address:

Olfactory ensheathing cells (OECs) are ideal candidates for cell-based therapies aimed at repairing spinal cord injury (SCI). Accurate targeting of OECs to the lesion site is critical to reconstructing the impaired nervous system. However, the key factors guiding the homing of transplanted OECs to the damaged area after SCI are still unclear. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.023DOI Listing
June 2019
4 Reads

ITGA6 and RPSA synergistically promote pancreatic cancer invasion and metastasis via PI3K and MAPK signaling pathways.

Exp Cell Res 2019 Jun 17;379(1):30-47. Epub 2019 Mar 17.

Department of General Surgery, Shengjing Hospital, China Medical University, Shenyang, Liaoning, PR China.

Pancreatic cancer is one of the most malignant tumors. Invasion and metastasis can occur in the early stage of pancreatic cancer, contributing to the poor prognosis. Accordingly, in this study, we evaluated the molecular mechanisms underlying invasion and metastasis. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.022DOI Listing
June 2019
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Human umbilical cord-derived mesenchymal stem cells enhanced HK-2 cell autophagy through MicroRNA-145 by inhibiting the PI3K/AKT/mTOR signaling pathway.

Exp Cell Res 2019 May 14;378(2):198-205. Epub 2019 Mar 14.

Department of Nephrology, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong Province, 510282, China. Electronic address:

Recent studies have shown that autophagy exhibits a protective role in acute kidney injury (AKI), and the accumulation of advanced oxidation protein products (AOPP) participates in the progression of kidney diseases. Our previous study indicated that AOPP induced injury in renal tubular epithelial cells (RTECs) through autophagy inhibition. Besides, we found that human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) enhanced autophagy in AOPP-treated RTECs, but the underlying mechanism remains unclear. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.019DOI Listing

DEPDC1B knockdown inhibits the development of malignant melanoma through suppressing cell proliferation and inducing cell apoptosis.

Exp Cell Res 2019 Jun 14;379(1):48-54. Epub 2019 Mar 14.

Department of Musculoskeletal Oncology, Fudan University Shanghai Cancer Center, Fudan University, Shanghai, China. Electronic address:

Malignant melanoma (MM) remains the leading cause of skin cancer related death, which has very poor prognosis because of locoregional recurrence and distant metastasis. DEPDC1B (DEP domain-containing protein 1B), has been proved to be associated with some types of malignant tumors. However, the role of DEPDC1B in MM is still unknown. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.021DOI Listing

Fusion and hemagglutinin proteins of canine distemper virus promote osteoclast formation through NF-κB dependent and independent mechanisms.

Exp Cell Res 2019 May 14;378(2):171-181. Epub 2019 Mar 14.

School of Public Health, Shandong University, Jinan, China. Electronic address:

Paget's disease (PD) features abnormal osteoclasts (OC) which sharply increase in number and size and then intensely induce bone resorption. The purpose of this study was to determine the direct effects of canine distemper virus (CDV) and its fusion protein and hemagglutinin protein (F + H) on receptor activator of nuclear factor kappa-B ligand (RANKL) induced OC formation in vitro. Immunofluorescence assay, OC morphological and functional detection, intracellular signaling pathway detection, Real-time PCR analysis and ELISA were applied in this study. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.020DOI Listing
May 2019
1 Read

MMP-12 regulates proliferation of mouse macrophages via the ERK/P38 MAPK pathways during inflammation.

Exp Cell Res 2019 May 14;378(2):182-190. Epub 2019 Mar 14.

Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, 56th Lingyuanxi Road, Guangzhou, Guangdong, 510055, China; Guangdong Province Key Laboratory of Stomatology, No. 74, 2nd Zhongshan Road, Guangzhou, 510080, Guangdong, China. Electronic address:

MMP-12 is a metalloproteinase (MMP) mainly secreted by macrophages and regulating the degradation of the extracellular matrix. MMP-12 is related to several diseases such as emphysema, myocardial infarction and liver fibrosis. However, the functions associated with inflammation of MMP-12 in macrophages have not yet been fully investigated. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.018DOI Listing
May 2019
4 Reads

From cell shape to cell fate via the cytoskeleton - Insights from the epidermis.

Exp Cell Res 2019 May 12;378(2):232-237. Epub 2019 Mar 12.

Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, Israel. Electronic address:

Animal cells exhibit a wide range of shapes that reflect their diverse functions. Cell shape is determined by a balance between internal and external forces and therefore involves the cytoskeleton and its associated adhesion structures. Cell shape dynamics during development and homeostasis are tightly regulated and closely coordinated with cell fate determination. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.016DOI Listing

Desmocollin 3 has a tumor suppressive activity through inhibition of AKT pathway in colorectal cancer.

Exp Cell Res 2019 May 8;378(2):124-130. Epub 2019 Mar 8.

Section Pathology of the Institute of Forensic Medicine, University Hospital Jena, Friedrich Schiller University Jena, Am Klinikum 1, 07747 Jena, Germany. Electronic address:

Desmocollin 3 (DSC3) is a transmembrane adhesion protein of desmosomes and involved in carcinogenesis in various cancer types. Downregulation of DSC3 has been reported in colorectal cancer (CRC). However, the function of DSC3 in CRC has not yet been elucidated. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144827193010
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http://dx.doi.org/10.1016/j.yexcr.2019.03.015DOI Listing
May 2019
8 Reads

Optimization of cell culture conditions for exosome isolation using mini-size exclusion chromatography (mini-SEC).

Exp Cell Res 2019 May 8;378(2):149-157. Epub 2019 Mar 8.

Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA; UPMC Hillman Cancer Center, Pittsburgh, PA 15213, USA; Departments of Immunology and Otolaryngology, Pittsburgh, PA 15213, USA. Electronic address:

Extracellular vesicles (EVs) are emerging as a major intercellular communication system engaged in a variety of physiological and pathophysiological processes. Tumor-derived exosomes (TEX) are a subset of EVs of special interest as potential cancer biomarkers. Supernatants of tumor cell lines are widely used as the source of pure TEX for molecular/genetic studies. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144827193010
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http://dx.doi.org/10.1016/j.yexcr.2019.03.014DOI Listing
May 2019
1 Read

CXCL12/CXCR4 pathway orchestrates CSC-like properties by CAF recruited tumor associated macrophage in OSCC.

Exp Cell Res 2019 May 8;378(2):131-138. Epub 2019 Mar 8.

School and Hospital of Stomatology, China Medical University, Shenyang 110002, PR China. Electronic address:

Tumor-associated macrophage (TAM), a crucial component of immune cell infiltrated in tumor microenvironment, is associated with progression of oral squamous cell carcinoma (OSCC). However, it is still unclear how TAM is induced/accumulated and activated around/in OSCC. In the study herein, we tried to understand how TAM accumulates and activates in the OSCC and how TAM promotes OSCC to convert cancer stem cell (CSC). Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.013DOI Listing
May 2019
2 Reads

MiR-1a-3p mitigates isoproterenol-induced heart failure by enhancing the expression of mitochondrial ND1 and COX1.

Exp Cell Res 2019 May 7;378(1):87-97. Epub 2019 Mar 7.

Department of Cardiology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China. Electronic address:

MicroRNAs (miRNAs) have become potential targets for the treatment of heart failure (HF). It has been shown that miR-1 can reverse cardiac hypertrophy during the compensatory phase of HF development, but it is unknown whether miR-1 can still reverse cardiac dysfunction and improve cardiac remodeling after HF progresses to the decompensation stage. We established a mouse model of isoproterenol-induced HF and then injected miR-1a-3p agomir (agomir-1) into the tail vein. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.012DOI Listing

Integrin intracellular machinery in action.

Exp Cell Res 2019 May 7;378(2):226-231. Epub 2019 Mar 7.

Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 3DY, United Kingdom. Electronic address:

Integrin-mediated adhesion to the extracellular matrix involves a surprisingly large number of intracellular proteins, the integrin-associated proteins (IAPs), which are a fraction of the total integrin adhesome. In this review we discuss how genetic approaches have improved our understanding of how each IAP contributes to integrin function, especially in the context of building a functional organism during development. We then begin the process of assembling IAP roles together into an integrated mechanism. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.03.011DOI Listing
May 2019
1 Read