21,810 results match your criteria Experimental Cell Research [Journal]


miR-29c regulates resistance to paclitaxel in nasopharyngeal cancer by targeting ITGB1.

Exp Cell Res 2019 Feb 16. Epub 2019 Feb 16.

Department of Oncology, Guizhou Provincial People's Hospital, Guiyang, Guizhou, China 2. Electronic address:

Paclitaxel (Taxol) is an anticancer taxane drug commonly used in the treatment of nasopharyngeal carcinoma (NPC). However, resistance to paclitaxel is a major difficulty in developing an effective therapy against NPC. MicroRNAs (miRNAs) are known to regulate genes that are involved in drug resistance. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.02.012DOI Listing
February 2019

Molecular Functions of Brain Expressed X-Linked 2 (BEX2) in Malignancies.

Authors:
Ali Naderi

Exp Cell Res 2019 Feb 16. Epub 2019 Feb 16.

University of Portsmouth, School of Pharmacy and Biomedical Sciences, White Swan Road, St. Michael's Building PO1 2DT, Portsmouth, United Kingdom; University of Hawaii Cancer Center, Cancer Biology Program, 701 Ilalo street, Honolulu, Hawaii 96813, USA. Electronic address:

Over the last decade there has been growing evidence that Brain Expressed X-Linked 2 (BEX2) has a significant role in the process of carcinogenesis. Collectively, available studies suggest a pro-oncogenic function for this gene in multiple malignancies, including breast, colorectal and hepatocellular cancers in addition to brain tumors. The identification of BEX2 in breast cancer resulted from gene expression microarray studies. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.02.014DOI Listing
February 2019

TRPC6 modulates adhesion of neutrophils to airway epithelial cells via NF-κB activation and ICAM-1 expression with ozone exposure.

Exp Cell Res 2019 Feb 16. Epub 2019 Feb 16.

Key Laboratory of Protein Modification and Degradation in School of Basic Medical Sciences, Affiliated Cancer Hospital & Institute, Guangzhou Medical University, Guangzhou, China. Electronic address:

Ozone (O) is a major component of air pollution, which has been associated with airway inflammation characterized by the influx of neutrophils in asthmatic subjects. Canonical transient receptor potential 6 (TRPC6) channel is recently identified as a target of oxidative stress which is involved in airway inflammation. However, the regulatory role of TRPC6 in airway epithelial cells and neutrophils has not yet been illuminated in detail. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.02.013DOI Listing
February 2019

Involvement of Stat3 phosphorylation in mild heat shock-induced thermotolerance.

Exp Cell Res 2019 Feb 15. Epub 2019 Feb 15.

Department of Biochemistry & Molecular Biology, Kyoto Pharmaceutical University, Kyoto 607-8414, Japan. Electronic address:

Thermotolerance is a phenomenon in which cells become resistant to stress by prior exposure to heat shock, and its development is associated with the induction of heat shock proteins (Hsps), including Hsp70. We previously showed that the expression of Hsp70 is regulated by the cytokine signaling transcription factor Stat3, but the role of Stat3 in thermotolerance is not known. In this study, we examined the possible involvement of Stat3 in the acquisition of thermotolerance. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.02.011DOI Listing
February 2019

α-Synuclein induced mitochondrial dysfunction via cytochrome c oxidase subunit 2 in SH-SY5Y cells.

Exp Cell Res 2019 Feb 15. Epub 2019 Feb 15.

Department of Neurology, The Sun Yat-sen Memorial Hospital of Sun Yat-sen University, 107 Yanjiang West Road, Guangzhou 510080, China; Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China; Guangdong Province Key Laboratory of Brain Function and Disease, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, China. Electronic address:

The transfer of misfolded α-Synuclein (α-Syn) from cell to cell as a prion protein is important in α-Synucleinopathies. Extraneous α-Syn induces apoptosis of dopaminergic neurons by causing mitochondrial dysfunction. However, the mechanism by which α-Syn disrupts the mitochondrial function is still unclear. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.02.006DOI Listing
February 2019

Examining the feasibility of a "top-down" approach to enhancing the keratinocyte-implant adhesion.

Exp Cell Res 2019 Feb 14. Epub 2019 Feb 14.

Department of Chemistry, Drexel University, Philadelphia, Pennsylvania 19104, United States. Electronic address:

The adhesion of human epidermal keratinocytes to the implant surface is one of the most critical steps during the patient's recovery from implantation of transcutaneous prosthesis. To improve the success rate of transcutaneous prosthetic implants, we explored a new "top-down" approach to promoting this dynamic adhering process through modulation of upstream cell signaling pathways. To examine the feasibility of this novel approach, we first established an in vitro platform that is capable of providing a non-invasive, real-time, quantitative characterization of the keratinocyte-implant interaction. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.01.024DOI Listing
February 2019

A SUV39H1-low chromatin state characterises and promotes migratory properties of cervical cancer cells.

Exp Cell Res 2019 Feb 14. Epub 2019 Feb 14.

National Centre for Biological Sciences, Tata Institute of Fundamental Research, GKVK-UAS, Bangalore, India. Electronic address:

Metastatic progression is a major cause of mortality in cervical cancers, but factors regulating migratory and pre-metastatic cell populations remain poorly understood. Here, we sought to assess whether a SUV39H1-low chromatin state promotes migratory cell populations in cervical cancers, using meta-analysis of data from The Cancer Genome Atlas (TCGA), immunohistochemistry, genomics and functional assays. Cervical cancer cells sorted based on migratory ability in vitro have low levels of SUV39H1 protein, and SUV39H1 knockdown in vitro enhanced cervical cancer cell migration. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.02.010DOI Listing
February 2019

Cytoophidia respond to nutrient stress in Drosophila.

Exp Cell Res 2019 Feb 12. Epub 2019 Feb 12.

School of Life Science and Technology, ShanghaiTech University, 230 Haike Road, 201210, Shanghai, China; Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3PT, United Kingdom. Electronic address:

CTP synthase (CTPsyn) is a metabolic enzyme essential for the de novo synthesis of CTP the nucleotide. CTPsyn can be compartmented into filamentous structures named cytoophidia. Cytoophidia are conserved in a wide range of species and are highly abundant in Drosophila ovaries. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.02.003DOI Listing
February 2019
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The role of nucleocytoplasmic transport in mechanotransduction.

Exp Cell Res 2019 Feb 12. Epub 2019 Feb 12.

Biozentrum and the Swiss Nanoscience Institute University of Basel, Klingelbergstrasse 70 CH-4056 Basel. Electronic address:

Cells integrate mechanical and biochemical signals via a process called mechanotransduction to generate essential gene expression patterns in space and time. This is vital for cell migration and proliferation as well as tissue morphogenesis and remodeling. While the force-sensing and force-transducing mechanisms are generally known, it remains unclear how mechanoresponsive transcription factors (TFs) are selectively translocated into the nucleus upon force-activation. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.02.009DOI Listing
February 2019

Homoharringtonine potentiates the antileukemic activity of arsenic trioxide against acute myeloid leukemia cells.

Exp Cell Res 2019 Feb 11. Epub 2019 Feb 11.

Central Laboratory, The Union Hospital of Fujian Medical University, Fuzhou, China. Electronic address:

Relapse of minimal residual disease (MRD) is a major problem after conventional chemotherapy in patients with acute myeloid leukemia (AML). The bone marrow stroma can protect AML cells from insults of chemotherapy, partly contributing to AML relapse. Arsenic trioxide (ATO) is the main component of arsenical traditional Chinese medicines and has been widely used for the treatment of hematologic malignancies particularly acute promyelocytic leukemia over the past three decades. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.02.008DOI Listing
February 2019
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Lipopolysaccharide mediates time-dependent macrophage M1/M2 polarization through the Tim-3/Galectin-9 signalling pathway.

Exp Cell Res 2019 Feb 11. Epub 2019 Feb 11.

Department of Critical Care Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, PR China. Electronic address:

Macrophages are dynamic cells whose phenotypes and functions are regulated by surrounding inflammatory mediators after pathogenic infection. Imbalanced polarization of classically activated (M1) and alternatively activated (M2) macrophages is closely associated with infection-related complications and their severity. The pathway of T-cell immunoglobulin mucin 3 (Tim-3)/galectin-9 (Gal-9) plays an important role in infection by regulating macrophage function. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.02.007DOI Listing
February 2019

Correlative light and electron microscopy is a powerful tool to study interactions of extracellular vesicles with recipient cells.

Exp Cell Res 2019 Feb 11. Epub 2019 Feb 11.

Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland. Electronic address:

Extracellular vesicles (EVs) and their interactions with recipient cells constitute a rapidly growing research area. However, due to the limitations in current methodologies, the mechanisms of these interactions are still unclear. Microscopic studies of EVs are challenging, because their typical diameter is near the resolution limit of light microscopy, and electron microscopy has restricted possibilities for protein labelling. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.02.004DOI Listing
February 2019

TRAF6-p38/JNK-ATF2 axis promotes microglial inflammatory activation.

Exp Cell Res 2019 Feb 11. Epub 2019 Feb 11.

Clinical Medicine Research Center, Affiliated Hospital 2 of Nantong University, Nantong 226001, People's Republic of China; Jiangsu Key Laboratory of Neurogeneration, Nantong University, Nantong 226001, People's Republic of China; Cancer Research Center of Nantong, Tumor Hospital Affiliated to Nantong University, Nantong 226361, People's Republic of China. Electronic address:

Activating transcription factor 2 (ATF2), a member of the alkaline-leucine zipper family, is widely expressed in various tissues, and reportedly involved in inflammatory responses to various irritates, but its role in the central nervous system (CNS) remains unclear. This study aimed to investigate the expression and biological function of ATF2 in CNS inflammation. Utilizing the LPS-induced neuroinflammation model on mice, we first found ATF2 up-regulation and its co-localization with microglia in inflamed mice brain. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.02.005DOI Listing
February 2019
3.246 Impact Factor

DA-Raf, a dominant-negative regulator of the Ras-ERK pathway, is essential for skeletal myocyte differentiation including myoblast fusion and apoptosis.

Exp Cell Res 2019 Feb 8. Epub 2019 Feb 8.

Department of Biology, Graduate School of Science, Chiba University, 1-33 Yayoicho, Inageku, Chiba 263-8522, Japan. Electronic address:

Ras-activated ERK pathway (Raf-MEK-ERK phosphorylation cascade) regulates a variety of cellular responses including cell proliferation, differentiation, survival, and apoptosis. DA-Raf1 (DA-Raf) is a splicing variant of A-Raf and contains the Ras-binding domain but lacks the kinase domain. Accordingly, DA-Raf antagonizes the Ras-ERK pathway in a dominant-negative manner. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.02.002DOI Listing
February 2019
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Role of Paip1 on angiogenesis and invasion in pancreatic cancer.

Exp Cell Res 2019 Feb 4. Epub 2019 Feb 4.

Department of Pathology & Cancer Research Center, Yanbian University Medical College, Yanji 133002, China; Key laboratory of the Science and Technology Department of Jilin Province, Yanji 133002, China. Electronic address:

Poly(A)-binding protein-interacting protein 1 (Paip1) was discovered as a modulator of translation initiation as a result of binding to PABP. Increasing evidence suggested that Paip1 has been implicated in several pathogenic roles. However, its clinical significance and underlying molecular mechanisms in Pancreatic cancer (PC) remain uncertain. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.01.017DOI Listing
February 2019

High expression of TAZ serves as a novel prognostic biomarker and drives cancer progression in renal cancer.

Exp Cell Res 2019 Feb 4. Epub 2019 Feb 4.

Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Institute of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. Electronic address:

Renal cell carcinomas are a group of most common renal malignancies whose clinical intervention is complicated by the lack of early diagnosis and reliable prognosis biomarkers, insensitive radiotherapy and chemotherapy and expensive molecular-targeted drugs. Transcriptional coactivator TAZ has been implicated in the formation and development of various malignancies. However, the biological characteristics and function of TAZ in renal cell carcinoma are still unclear. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.02.001DOI Listing
February 2019
1 Read
3.246 Impact Factor

Activation mechanisms and multifaceted effects of mast cells in ischemia reperfusion injury.

Exp Cell Res 2019 Feb 1. Epub 2019 Feb 1.

Department of General Surgery, Shanghai Tenth People's Hospital School of Medicine, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, PR China. Electronic address:

Mast cells (MCs) are tissue resident effector cells that form an important part of the immune system's first-line of defence against various pathogenic challenges. They are well known for their roles in anaphylaxis and allergy; however, increasing evidence implicates MCs in a wide range of pathologies. Ischemia/reperfusion (I/R) injury elicits an inflammatory response and triggers the program of tissue damage and restoration, as well as immune regulation. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.01.022DOI Listing
February 2019
2 Reads

GXYLT2 accelerates cell growth and migration by regulating the Notch pathway in human cancer cells.

Exp Cell Res 2019 Mar 1;376(1):1-10. Epub 2019 Feb 1.

Department of Medical Genetics, Peking University School of Basic Medical Sciences, Beijing 100191, China; Peking University Center for Human Disease Genomics, Beijing 100191, China. Electronic address:

Glucoside xylosyltransferase2 (GXYLT2), a member of the human α-1,3-D-xylosyltransferases, functions to modify the first xylose to the O-Glucose residue on epidermal growth factor (EGF) repeats of Notch receptors. It is well-established that the Notch signaling pathway plays a critical role in proper development and homeostasis. However, the regulatory role of EGF xylosylation in Notch signaling and different cell activities in human cells remains unknown. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.01.023DOI Listing
March 2019
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Differential contributions of nonmuscle myosin IIA and IIB to cytokinesis in human immortalized fibroblasts.

Exp Cell Res 2019 Mar 31;376(1):67-76. Epub 2019 Jan 31.

Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo 060-8628, Japan; Department of Chemistry, Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan. Electronic address:

Nonmuscle myosin II (NMII) plays an important role in cytokinesis by constricting a contractile ring. However, it is poorly understood how NMII isoforms contribute to cytokinesis in mammalian cells. Here, we investigated the roles of the two major NMII isoforms, NMIIA and NMIIB, in cytokinesis using a WI-38 VA13 cell line (human immortalized fibroblast). Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.01.020DOI Listing

IL-10-producing B cells in differentiated thyroid cancer suppressed the effector function of T cells but improved their survival upon activation.

Exp Cell Res 2019 Jan 31. Epub 2019 Jan 31.

Department of Nuclear Medicine, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, China.

Despite having favorable prognosis, patients with differentiated thyroid carcinoma (DTC) still suffer from reduced lifespan due to recurrences, metastasis, and dedifferentiation. Regulatory B (Breg) cells are essential to the maintenance of peripheral tolerance, but upregulated Breg response may impede the clearance of pathogens and tumor cells. Here, we found that in PBMCs, the frequency of IL-10 circulating B cells ex vivo and following stimulation was similar between DTC patients and healthy controls. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.01.021DOI Listing
January 2019
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CSN5 attenuates Ang II-induced cardiac hypertrophy through stabilizing LKB1.

Exp Cell Res 2019 Mar 30;376(1):11-17. Epub 2019 Jan 30.

Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China. Electronic address:

CSN5 is a critical subunit of the COP9 signalosome (CSN) and has been involved in various cellular processes, but little is known about the role of CSN5 in cardiac disease. In the present study, we found that the expression of CSN5 was increased in Angiotensin II (Ang II)-induced cardiac hypertrophic mice hearts and Ang II-treated cardiomyocytes. We also observed that overexpression of CSN5 significantly inhibited Ang II-induced cardiac hypertrophy, whereas CSN5 silence exhibited the opposite phenotypes. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144827183114
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http://dx.doi.org/10.1016/j.yexcr.2019.01.019DOI Listing
March 2019
1 Read

Matrix metalloproteinase 9 inhibits the motility of highly aggressive HSC-3 oral squamous cell carcinoma cells.

Exp Cell Res 2019 Mar 30;376(1):18-26. Epub 2019 Jan 30.

Cancer and Translational Medicine Research Unit, Faculty of Medicine, University of Oulu, Oulu, Finland; Medical Research Center Oulu, Oulu University Hospital, University of Oulu, Oulu, Finland; Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland; HUSLAB, Department of Pathology, Helsinki University Central Hospital, University of Helsinki, Helsinki, Finland. Electronic address:

Pro-tumorigenic activities of matrix metalloproteinase (MMP) 9 have been linked to many cancers, but recently the tumour-suppressing role of MMP9 has also been elucidated. The multifaceted evidence on this subject prompted us to examine the role of MMP9 in the behaviour of oral tongue squamous cell carcinoma (OTSCC) cells. We used gelatinase-specific inhibitor, CTT2, and short hairpin (sh) RNA gene silencing to study the effects of MMP9 on proliferation, motility and invasion of an aggressive OTSCC cell line, HSC-3. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.01.018DOI Listing

Growth suppression of human oral cancer cells by candidate agents for cetuximab-side effects.

Exp Cell Res 2019 Jan 25. Epub 2019 Jan 25.

Department of Oral Science, Graduate School of Medicine, Chiba University, 1-8- 1 Inohana, Chuo-ku, Chiba 260-8670, Japan; Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, 1-8-1 Inohana, Chuo-ku, Chiba 260-8677, Japan.

Cetuximab, an inhibitor of the epidermal growth factor receptor that is used widely to treat human cancers including oral squamous cell carcinoma (OSCC), has characteristic side effects of skin rash and hypomagnesemia. However, the mechanisms of and therapeutic agents for skin rashes and hypomagnesemia are still poorly understood. Our gene expression profiling analyses showed that cetuximab activates the p38 MAPK pathways in human skin cells (human keratinocyte cell line [HaCaT]) and inhibits c-Fos-related signals in human embryonic kidney cells (HEK293). Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.01.016DOI Listing
January 2019

Notch1 inhibition enhances DNA damage induced by cisplatin in cervical cancer.

Exp Cell Res 2019 Mar 26;376(1):27-38. Epub 2019 Jan 26.

The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Changchun 130021, China. Electronic address:

The expression of Notch1 plays an important role in the occurrence and development of various tumors. Previous studies have shown that Notch1 plays a negative regulatory role in response to radiation-induced DNA damage responses. It also has been reported that Notch1 was highly expressed in cervical cancer. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.01.014DOI Listing

CTGF regulates cyclic stretch-induced vascular smooth muscle cell proliferation via microRNA-19b-3p.

Exp Cell Res 2019 Mar 25;376(1):77-85. Epub 2019 Jan 25.

Institute of Mechanobiology & Medical Engineering, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, P.O.Box 888, 800 Dongchuan Road, Minhang, Shanghai 200240, China. Electronic address:

Cyclic stretch regulates proliferation of vascular smooth muscle cells (VSMCs) during hypertension-induced vascular remodeling, but the underlying mechanisms remain to be studied. Connective tissue growth factor (CTGF) has been reported associated with several cellular function such as proliferation,migration and adhesion. Herein, the role of CTGF in VSMCs was investigated in response to mechanical cyclic stretch. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.01.015DOI Listing

p53 mediates hydroxyurea resistance in aneuploid cells of colon cancer.

Exp Cell Res 2019 Mar 23;376(1):39-48. Epub 2019 Jan 23.

Jiangsu Key Laboratory of Experimental & Translational Non-coding RNA Research, Yangzhou University, Yangzhou 225001, China; Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou 225001, China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou, China. Electronic address:

Aneuploidy refers to aberrancies in cellular chromosome count, which is prevalent in most human cancers. Chemotherapy is an effective cancer treatment; however, the development of drug resistance is a major concern of conventional chemotherapy. The chemotherapy agent hydroxyurea (HU) targets proliferating cells and has long been applied to treat various human cancers. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.01.013DOI Listing
March 2019
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Migration versus proliferation as contributor to in vitro wound healing of vascular endothelial and smooth muscle cells.

Exp Cell Res 2019 Mar 17;376(1):58-66. Epub 2019 Jan 17.

Department of Biomedical Engineering, College of Engineering, University of Arizona,1127 E James E Rogers Way, PO Box 210020, Tucson, AZ 85721, USA; Department of Medicine, Sarver Heart Center, University of Arizona, 1501 N Campbell Ave, PO Box 245035, Tucson, AZ 85724, USA. Electronic address:

Wound closure, as a result of collective cell growth, is an essential biological response to injury. In the field of vascular biology, the response of vascular smooth muscle cells (SMCs) and endothelial cells (ECs) to injury and substrate surface is important in therapeutic clinical treatment interventions such as angioplasty and atherectomy. Specifically, the mechanism by which cells close wounds (i. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144827183109
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http://dx.doi.org/10.1016/j.yexcr.2019.01.011DOI Listing
March 2019
6 Reads
3.246 Impact Factor

Cell-matrix tension contributes to hypoxia in astrocyte-seeded viscoelastic hydrogels composed of collagen and hyaluronan.

Exp Cell Res 2019 Mar 15;376(1):49-57. Epub 2019 Jan 15.

Department of Biomedical Engineering, Rowan University, Glassboro, NJ 08028, United States.

Astrocyte activation is crucial for wound contraction and glial scar formation following central nervous system injury, but the mechanism by which activation leads to astrocyte contractility and matrix reorganization in the central nervous system (CNS) is unknown. Current means to measure cell traction forces within three-dimensional scaffolds are limited to analyzing individual or small groups of cells, within extracellular matrices, whereas gap junctions and other cell-cell adhesions connect astrocytes to form a functional syncytium within the glial scar. Here, we measure the viscoelastic properties of cell-seeded hydrogels to yield insight into the collective contractility of astrocytes as they exert tension on the surrounding matrix and change its bulk mechanical properties. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144827193001
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http://dx.doi.org/10.1016/j.yexcr.2019.01.012DOI Listing
March 2019
7 Reads

Proteomic profile of TGF-β1 treated lung fibroblasts identifies novel markers of activated fibroblasts in the silica exposed rat lung.

Exp Cell Res 2019 Feb 11;375(2):1-9. Epub 2019 Jan 11.

Basic Medical College, North China University of Science and Technology, Tangshan, China; School of public health, North China University of Science and Technology, Tangshan, China; The Hebei key laboratory for organ fibrosis research, North China University of Science and Technology, Tangshan, China. Electronic address:

We performed liquid chromatography-tandem mass spectrometry (LC-MS/MS) on control and TGF-β1-exposed rat lung fibroblasts to identify proteins differentially expressed between cell populations. A total of 196 proteins were found to be differentially expressed in response to TGF-β1 treatment. Guided by these results, we next determined whether similar changes in protein expression were detectable in the rat lung after chronic exposure to silica dust. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.01.010DOI Listing
February 2019
5 Reads

Inhibition of miRNA-21 promotes retinal ganglion cell survival and visual function by modulating Müller cell gliosis after optic nerve crush.

Exp Cell Res 2019 Feb 9;375(2):10-19. Epub 2019 Jan 9.

Department of Neurosurgery, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai 201999, China; Institute of Traumatic Medicine, Shanghai JiaoTong University School of Medicine, Shanghai 201999, China. Electronic address:

Background: Müller cell gliosis not only plays an important physiological role by maintaining retinal neuronal homeostasis but is also associated with multiple pathological events in the retina, including optic nerve crush (ONC) injury. Modulating Müller cell gliosis contributes to the creation of a permissive environment for neuronal survival. However, the underlying mechanism of Müller cell gliosis has remained elusive. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144827193001
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http://dx.doi.org/10.1016/j.yexcr.2019.01.009DOI Listing
February 2019
5 Reads

Sox7 is involved in antibody-dependent endothelial cell activation and renal allograft injury via the Jagged1-Notch1 pathway.

Exp Cell Res 2019 Feb 9;375(2):20-27. Epub 2019 Jan 9.

Department of Kidney Transplantation & Urology, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200080, China. Electronic address:

Background: Antibody-mediated rejection (AMR) can cause graft loss and reduces long-term graft survival after kidney transplantation. Human leukocyte antigen (HLA) and/or non-HLA antibodies play a key role in the pathogenesis of AMR by targeting the allograft epithelium via complement activation and complement-independent mechanisms. However, the precise mechanisms of AMR remain unclear and treatment is still limited. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.01.008DOI Listing
February 2019
2 Reads

Cadherin mechanotransduction in leader-follower cell specification during collective migration.

Exp Cell Res 2019 Mar 8;376(1):86-91. Epub 2019 Jan 8.

Dept. Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, Stratenum 3.231, Universiteitsweg 100, 3584 CG, Utrecht, the Netherlands. Electronic address:

Collective invasion drives the spread of multicellular cancer groups, into the normal tissue surrounding several epithelial tumors. Collective invasion recapitulates various aspects of the multicellular organization and collective migration that take place during normal development and repair. Collective migration starts with the specification of leader cells in which a polarized, migratory phenotype is established. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144827183111
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http://dx.doi.org/10.1016/j.yexcr.2019.01.006DOI Listing
March 2019
4 Reads

Translocating transcription factors in fluid shear stress-mediated vascular remodeling and disease.

Exp Cell Res 2019 Mar 8;376(1):92-97. Epub 2019 Jan 8.

Department of Cell Biology, Yale School of Medicine, United States; Yale Cardiovascular Research Center, United States; Department of Medicine (Cardiology), United States; Department of Biomedical Engineering, United States. Electronic address:

Endothelial cells are exposed to fluid shear stress profiles that vary in magnitude, pulsatility, and directionality due to regional variations in blood vessel structure. Laminar flow at physiological levels is atheroprotective; multidirectional or reversing low (disturbed) flow promotes inflammation and disease; and high or low laminar flow promote outward or inward remodeling, respectively. However, our understanding of how endothelial cells discern these different flow profiles and regulate gene expression accordingly is limited. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144827183099
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http://dx.doi.org/10.1016/j.yexcr.2019.01.005DOI Listing
March 2019
8 Reads

The "nuclear physics" behind epigenetic control of cell fate.

Exp Cell Res 2019 Jan 8. Epub 2019 Jan 8.

King's College London, School of Basic & Medical Biosciences, Centre for Stem Cells & Regenerative Medicine, Guy's Hospital, Great Maze Pond, Tower Wing, 28th Floor, London SE1 9RT, UK. Electronic address:

It is well understood that replicative and transcriptional responses in the nucleus occur under the influence of specific extracellular biochemical signals (e.g. growth factors and cytokines). Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.01.007DOI Listing
January 2019
1 Read

Corrigendum to "Deficiency of CKIP-1 aggravates high-fat diet-induced fatty liver in mice" [Exp. Cell Res. 355(1) (2017) 40-46].

Exp Cell Res 2019 Feb 7;375(1):97. Epub 2019 Jan 7.

Department of Gastroenterology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.

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https://linkinghub.elsevier.com/retrieve/pii/S00144827183118
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http://dx.doi.org/10.1016/j.yexcr.2018.12.026DOI Listing
February 2019
11 Reads
3.246 Impact Factor

The catalytic subunit of DNA polymerase δ is a nucleocytoplasmic shuttling protein.

Exp Cell Res 2019 Feb 6;375(2):36-40. Epub 2019 Jan 6.

HKUST Shenzhen Research Institute and Shenzhen Key Laboratory of Edible & Medicinal Bioresources, Shenzhen, China; Division of Life Science and State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China. Electronic address:

The DNA polymerase δ catalytic subunit (PolD1) is a highly conserved protein with established functions in both the nucleus and the cytoplasm: whereas PolD1 participates in the replication and repair of nuclear DNA, it plays a role in the control of cytoplasmic microtubule growth by directly acting on microtubule-nucleator γ-tubulin ring complexes. Here, we show that PolD1 shuttles between the nucleus and the cytoplasm. PolD1 harbors two nuclear localization signals that mediate the active transport of PolD1 to the nucleus; conversely, PolD1 is exported from the nucleus by the exportin CRM1-dependent mechanism, a major nuclear-export pathway that mediates the export of various cargos. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144827183103
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http://dx.doi.org/10.1016/j.yexcr.2019.01.003DOI Listing
February 2019
8 Reads

Endocrine and exocrine pancreas pathologies crosstalk: Insulin regulates the unfolded protein response in pancreatic exocrine acinar cells.

Exp Cell Res 2019 Feb 6;375(2):28-35. Epub 2019 Jan 6.

Department of Nutrition, Faculty of Health Sciences, Ariel University, 40700, Israel. Electronic address:

Exocrine pancreas insufficiency is common in diabetic mellitus (DM) patients. Cellular stress is a prerequisite in the development of pancreatic pathologies such as acute pancreatitis (AP). The molecular mechanisms underlying exocrine pancreatic ER-stress in DM are largely unknown. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144827193000
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http://dx.doi.org/10.1016/j.yexcr.2019.01.004DOI Listing
February 2019
7 Reads

Autophagy is involved in the differentiation of epicardial progenitor cells into vascular smooth muscle cells in mice.

Exp Cell Res 2019 Feb 3;375(2):60-71. Epub 2019 Jan 3.

Department of Cardiology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China. Electronic address:

Most coronary smooth muscle cells(CoSMCs) are differentiated from epicardial progenitor cells(EPCs), but the specific mechanism is not fully investigated. Previous studies have shown that autophagy plays an important role for smooth muscle cells(SMCs) differentiation, yet whether autophagy is involved in the differentiation of EPCs into CoSMCs remains unclear. In the present study, We first isolated and cultured EPCs and continuously cultured them for 72 h. Read More

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http://dx.doi.org/10.1016/j.yexcr.2018.12.025DOI Listing
February 2019
1 Read

Dual role of twist1 in cancer-associated fibroblasts and tumor cells promoted epithelial-mesenchymal transition of esophageal cancer.

Exp Cell Res 2019 Feb 3;375(2):41-50. Epub 2019 Jan 3.

Department of Thoracic Surgery, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450000, Henan, China; Zhengzhou University School of Medicine, Zhengzhou 450000, Henan, China. Electronic address:

Cancer-associated fibroblasts (CAFs) play critical roles in tumor progression. However, the role and mechanism underlying CAFs in esophageal cancer (EC) remain unclear. In this study, primary CAFs and normal esophageal fibroblasts (NOFs) were isolated and characterized by immunofluorescence, qRT-PCR and western blot. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144827193000
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http://dx.doi.org/10.1016/j.yexcr.2019.01.002DOI Listing
February 2019
3 Reads
3.246 Impact Factor

Implications of scar structure and mechanics for post-infarction cardiac repair and regeneration.

Exp Cell Res 2019 Mar 2;376(1):98-103. Epub 2019 Jan 2.

Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA; Department of Medicine, University of Virginia, Charlottesville, VA, USA; Robert M. Berne Cardiovascular Research Center, University of Virginia, Charlottesville, VA, USA. Electronic address:

Regenerating cardiac muscle lost during a heart attack is a topic of broad interest and enormous potential impact. One promising approach is to regenerate or re-engineer new myocardium in situ, at the site of damage, by injecting cells, growth factors, and other materials, or by reprogramming aspects of the normal wound healing process. A wide variety of strategies have been explored, from promoting angiogenesis to injection of a variety of different progenitor cell types, to re-engineering resident cells to produce key growth factors or even transdifferentiate into myocytes. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.01.001DOI Listing
March 2019
1 Read

Toll-like receptor 9 signaling promotes autophagy and apoptosis via divergent functions of the p38/JNK pathway in human salivary gland cells.

Exp Cell Res 2019 Feb 3;375(2):51-59. Epub 2019 Jan 3.

Department of Oral Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, China; National Clinical Research Center of Oral Disease, China; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, China. Electronic address:

Abnormal signaling transduction in salivary gland cells is associated with the pathogenesis of Sjögren's syndrome (SS). Previously, we identified aberrant expression of toll-like receptor 9 (TLR9) in gland cells of SS patients and mouse models. In this study, we investigated the role of TLR9 and its downstream p38/mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) signaling in mediating apoptosis and autophagy in human salivary gland (HSG) cells. Read More

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http://dx.doi.org/10.1016/j.yexcr.2018.12.027DOI Listing
February 2019
3 Reads

uPA affects CRSsNP nasal mucosa epithelium apoptosis by regulating wif1.

Exp Cell Res 2018 Dec 31. Epub 2018 Dec 31.

Department of Otolaryngology, Shanghai General Hospital, Shanghai Jiaotong University. No 100, Haining Road, Shanghai, China. Electronic address:

Chronic rhinosinusitis without nasal polyps (CRSsNP) is the main type of chronic rhinosinusitis (CRS) and is a common otorhinolaryngologic disease worldwide. However, the mechanisms of CRSsNP remain poorly understood. In this study, C57BL/6J wild-type and urokinase-type plasminogen activator (uPA) gene knockout (uPA) mice were used to establish a CRSsNP model. Read More

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http://dx.doi.org/10.1016/j.yexcr.2018.12.024DOI Listing
December 2018
2 Reads

Low-level shear stress induces differentiation of liver cancer stem cells via the Wnt/β-catenin signalling pathway.

Exp Cell Res 2019 Feb 29;375(1):90-96. Epub 2018 Dec 29.

Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, People's Republic of China. Electronic address:

Shear stress-activated cellular signalling is involved in cellular migration, proliferation and differentiation. However, it remains unclear about the effects of shear stress on the differentiation of liver cancer stem cells (LCSCs). In this study, using a parallel plated flow chamber system, we found that low-shear stress loading (2 dyne/cm) for 48 h could significantly inhibit the sphere-forming ability, increase the chemotherapeutic drug sensitivity, downregulate cancer stem cell marker expression and suppress the in vivo tumorigenicity potential of LCSCs. Read More

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http://dx.doi.org/10.1016/j.yexcr.2018.12.023DOI Listing
February 2019
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Retraction notice to "The Piezo1 Protein Ion Channel functions in Human Nucleus Pulposus Cell Apoptosis by regulating Mitochondrial Dysfunction and the Endoplasmic Reticulum Stress Signal Pathway"[Experimental Cell Research 358 (2017) 377-389].

Exp Cell Res 2019 01;374(2):374

Department of Joint Surgery, The Affiliated Hospital of Qingdao University, 59 Haier Road, Shandong 266000, People's Republic of China.

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http://dx.doi.org/10.1016/j.yexcr.2018.12.019DOI Listing
January 2019
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Promoting neural transdifferentiation of BMSCs via applying synergetic multiple factors for nerve regeneration.

Exp Cell Res 2019 Feb 29;375(2):80-91. Epub 2018 Dec 29.

School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, United States.

The strategy of using multipotential stem cells like bone mesenchymal stromal cells (BMSCs) for nerve tissue engineering is proven feasible. The promotion effects on neural transdifferentiation of BMSCs from factors including nerve growth factor (NGF), laminin and electrical stimulation (ES) have been reported, while it is not known if these factors can achieve a strong synergetic impact when the cells are cultured on conductive substrates. In this study, it was identified that any single factor (NGF, laminin, or conductive substrate) combined with ES demonstrated the capacity to induce BMSCs transdifferentiating into neural cells, while the efficiency was found in the order of NGF > laminin > conductive substrate. Read More

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http://dx.doi.org/10.1016/j.yexcr.2018.12.021DOI Listing
February 2019
2 Reads

Wild-type and mutant (G2019S) leucine-rich repeat kinase 2 (LRRK2) associate with subunits of the translocase of outer mitochondrial membrane (TOM) complex.

Exp Cell Res 2019 Feb 28;375(2):72-79. Epub 2018 Dec 28.

Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa. Electronic address:

Leucine-rich repeat kinase 2 (LRRK2) is important in various cellular processes including mitochondrial homeostasis and mutations in this gene lead to Parkinson's disease (PD). However, the full spectrum of LRRK2's functions remain to be elucidated. The translocase of outer mitochondrial membrane (TOM) complex is essential for the import of almost all nuclear-encoded mitochondrial proteins and is fundamental for cellular survival. Read More

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http://dx.doi.org/10.1016/j.yexcr.2018.12.022DOI Listing
February 2019
3 Reads

MeCP2 promotes endothelial-to-mesenchymal transition in human endothelial cells by downregulating BMP7 expression.

Exp Cell Res 2019 Feb 28;375(1):82-89. Epub 2018 Dec 28.

Department of Geriatrics, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, 1665 Kong Jiang Road, Shanghai, China. Electronic address:

Endothelial-to-mesenchymal transition (EndMT) plays a pivotal role in the development of organ fibrosis and can be induced by TGF-β. It is characterized by the loss of endothelial cell markers and the acquisition of mesenchymal markers. In this study, we found that methyl-CpG binding protein 2 (MeCP2) was increased in TGF-β-induced EndMT, and silencing of MeCP2 inhibited EndMT induction. Read More

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http://dx.doi.org/10.1016/j.yexcr.2018.12.020DOI Listing
February 2019
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Silencing of Gal-7 inhibits TGF-β-induced apoptosis of human airway epithelial cells through JNK signaling pathway.

Exp Cell Res 2019 Feb 27;375(2):100-105. Epub 2018 Dec 27.

Department of Hematopathology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, PR China. Electronic address:

Apoptosis of epithelial cells is regarded as the initial pathological process of many lung diseases, including asthma. Previous studies have identified that galectin-7 (Gal-7), a regulator of apoptosis, was overexpressed in bronchial epithelial cells in asthma. However, the effect and mechanism of Gal-7 in the progression of asthma is still unclear. Read More

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http://dx.doi.org/10.1016/j.yexcr.2018.12.017DOI Listing
February 2019
2 Reads

MiR-7-5p suppresses stemness and enhances temozolomide sensitivity of drug-resistant glioblastoma cells by targeting Yin Yang 1.

Exp Cell Res 2019 Feb 23;375(1):73-81. Epub 2018 Dec 23.

Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. Electronic address:

Glioblastoma multiforme (GBM) is the most malignant tumor of the central nervous system, and chemoresistance blunts the effect of temozolomide (TMZ) in the treatment of GBM. Clarifying the underlying mechanism of chemoresistance might yield novel strategies to improve the patients' response to chemotherapeutics. Mounting evidence indicates that microRNAs (miRNAs) are involved in chemoresistance and tumorigenesis. Read More

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http://dx.doi.org/10.1016/j.yexcr.2018.12.016DOI Listing
February 2019
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3.246 Impact Factor

Comprehensive characterization of RB1 mutant and MYCN amplified retinoblastoma cell lines.

Exp Cell Res 2019 Feb 22;375(2):92-99. Epub 2018 Dec 22.

Institute of Human Genetics, University Hospital Essen, University Duisburg-Essen, Hufelandstr.55, 45147 Essen, Germany. Electronic address:

In retinoblastoma research tumor-derived cell lines remain an important model to investigate tumorigenesis and new therapy options, due to limited tumor material and lack of adequate animal models. A panel of 10 retinoblastoma cell lines was characterized with respect to mutation, methylation and expression of RB1 and MYCN. These established retinoblastoma cell lines represent the most frequent types of RB1 inactivation and together with the MYCN amplification status, three classes can be distinguished: RB1/MYCN, RB1/MYCN and RB1/MYCN. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144827183066
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http://dx.doi.org/10.1016/j.yexcr.2018.12.018DOI Listing
February 2019
4 Reads